Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
transcriptional changes in innate immunity genes in head kidneys from aeromonas salmonicida-challenged rainbow trout fed a mixture of polycyclic aromatic hydrocarbons.we previously observed that exposure to a complex mixture of high molecular weight polycyclic aromatic hydrocarbons (pahs) increased sensitivity of rainbow trout (oncorhynchus mykiss) to subsequent challenge with aeromonas salmonicida, the causative agent of furunculosis. in this study, we evaluate potential mechanisms associated with disease susceptibility from combined environmental factors of dietary pah exposure and pathogen challenge. rainbow trout were fed a mixture of ten high molecular w ...201728407501
the role for the small cryptic plasmids as moldable vectors for genetic innovation in aeromonas salmonicida subsp. salmonicida.in aeromonas salmonicida subsp. salmonicida, a bacterium that causes fish disease, there are two types of small plasmids (<15 kbp): plasmids without known function, called cryptic plasmids, and plasmids that bear beneficial genes for the bacterium. four among them are frequently detected in strains of a. salmonicida subsp. salmonicida: pasa1, pasa2, pasa3, and pasal1. the latter harbors a gene which codes for an effector of the type three secretion system, while the three others are cryptic. it ...201729326751
characterization of the cultivable gut microflora in wild-caught 
mediterranean fish species.microflora of the gastrointestinal tract plays important roles in food digestion, nutrient absorption and in host defense against ingested pathogens. several studies have focused on the microflora of farmed fishes, but the gut flora of wild fishes remains poorly characterized. the aim of this work was to provide an overview of the bacteria colonizing the gut of wild-caught fishes and to determine whether some bacterial species can be pathogenic.201728553195
in vitro assessment of the antimicrobial activity of silver and zinc oxide nanoparticles against fish pathogens.antibiotic resistance is a global issue that threatens public health. the excessive use of antibiotics contributes to this problem as the genes of antibiotic resistance can be transferred between the bacteria in humans, animals and aquatic organisms. metallic nanoparticles could serve as future substitutes for some conventional antibiotics because of their antimicrobial activity. the aim of this study was to evaluate the antimicrobial effects of silver and zinc oxide nanoparticles against major ...201728732514
phenotype of aeromonas salmonicida sp. salmonicida cyclic adenosine 3',5'-monophosphate receptor protein (crp) mutants and its virulence in rainbow trout (oncorhynchus mykiss).precise deletion of genes related to virulence can be used as a strategy to produce attenuated bacterial vaccines. here, we study the deletion of the cyclic-3',5'-adenosine monophosphate (camp) receptor protein (crp) in aeromonas salmonicida, the aetiologic agent of furunculosis in marine and freshwater fish. the crp protein is a conserved global regulator, controlling physiology processes, like sugar utilization. deletion of the crp gene has been utilized in live attenuated vaccines for mammals ...201728548689
studies on the antibody response and side effects after intramuscular and intraperitoneal injection of atlantic lumpfish (cyclopterus lumpus l.) with different oil-based vaccines.atlantic lumpfish (cyclopterus lumpus l.) is used as a biological delousing agent for sea lice (lepeophtheirus salmonis k.) infestations in norwegian aquaculture. here, we present a study on the antibody response and vaccine side effects after intramuscular and intraperitoneal injection of lumpfish with two vaccines. both vaccines contained bacterial antigens from atypical aeromonas salmonicida a-layer types v and vi, vibrio anguillarum serotype o1 and moritella viscosa sp., but one vaccine cont ...201728548686
characterization and diversity of phages infecting aeromonas salmonicida subsp. salmonicida.phages infecting aeromonas salmonicida subsp. salmonicida, the causative agent of the fish disease furunculosis, have been isolated for decades but very few of them have been characterized. here, the host range of 12 virulent phages, including three isolated in the present study, was evaluated against a panel of 65 a. salmonicida isolates, including representatives of the psychrophilic subspecies salmonicida, smithia, masoucida, and the mesophilic subspecies pectinolytica. this bacterial set als ...201728765570
chromosome-mediated mcr-3 variants in aeromonas veronii from chicken meat.two adjacent colistin resistance gene variants, termed mcr-3.2 and mcr-3-like, were identified in the chromosome of an aeromonas veronii isolated from retail chicken meat. the variants showed 95.20% and 84.19% nucleotide sequence identity, respectively, to mcr-3 from porcine escherichia coli functional cloning indicated that only mcr-3.2 conferred polymyxin resistance in both e. coli and aeromonas salmonicida the mcr-3.2-mcr-3-like segment was also observed in other aeromonas species, including ...201728848017
aeromonas salmonicida growth in response to atlantic salmon mucins differs between epithelial sites, is governed by sialylated and n-acetylhexosamine-containing o-glycans, and is affected by ca(2).aeromonas salmonicida causes furunculosis in salmonids and is a threat to atlantic salmon aquaculture. the epithelial surfaces that the pathogen colonizes are covered by a mucus layer predominantly comprised of secreted mucins. by using mass spectrometry to identify mucin glycan structures with and without enzymatic removal of glycan residues, coupled to measurements of bacterial growth, we show here that the complex atlantic salmon intestinal mucin glycans enhance a. salmonicida growth, whereas ...201728533470
in vitro effects of italian lavandula multifida l. leaf extracts on gilthead seabream (sparus aurata) leucocytes and saf-1 cells.lavandula multifida is very appreciated by pharmaceutical and cosmetic industries. in italy is only found in calabria and sicily and, at present, urge its valorization due to its high extinction and genetic erosion risks. possible applications of l. multifida extracts as immunostimulant in fish aquaculture were assayed by using gilthead seabream (sparus aurata) as a marine fish model, due to its importance in fish aquaculture. the in vitro effects of both aqueous and ethanolic leaf extracts obta ...201728522420
differential effects of exposure to parasites and bacteria on stress response in turbot scophthalmus maximus simultaneously stressed by low water depth.the stress response of turbot scophthalmus maximus was evaluated in fish maintained 8 days under different water depths, normal (nwd, 30 cm depth, total water volume 40 l) or low (lwd, 5 cm depth, total water volume 10 l), in the additional presence of infection-infestation of two pathogens of this species. this was caused by intraperitoneal injection of sublethal doses of the bacterium aeromonas salmonicida subsp. salmonicida or the parasite philasterides dicentrarchi (ciliophora:scuticociliati ...201728516502
the protective effect of humic-rich substances on atypical aeromonas salmonicida subsp. salmonicida infection in common carp (cyprinus carpio l.).when challenged with atypical aeromonas salmonicida subsp. salmonicida, exposure of the common carp (cyprinus carpio l.) to different humic-rich compounds resulted in a significant reduction in infection rates. specifically, in fish exposed to (i) humic-rich water and sludge from a recirculating system, (ii) a synthetic humic acid, and (iii) a leonardite-derived humic-rich extract, infection rates were reduced to 14.9%, 17.0% and 18.8%, respectively, as compared to a 46.8% infection rate in the ...201728493490
a historical review of the key bacterial and viral pathogens of scottish wild fish.thousands of scottish wild fish were screened for pathogens by marine scotland science. a systematic review of published and unpublished data on six key pathogens (renibacterium salmoninarum, aeromonas salmonicida, ipnv, isav, sav and vhsv) found in scottish wild and farmed fish was undertaken. despite many reported cases in farmed fish, there was a limited number of positive samples from scottish wild fish, however, there was evidence for interactions between wild and farmed fish. a slightly el ...201728718925
microplastics as a vector for the transport of the bacterial fish pathogen species aeromonas salmonicida.microplastics is widespread in the marine environment where it can cause numerous negative effects. it can provide space for the growth of organisms and serves as a vector for the long distance transfer of marine microorganisms. in this study, we examined the sea surface concentrations of microplastics in the north adriatic and characterized bacterial communities living on the microplastics. dna from microplastics particles was isolated by three different methods, followed by pcr amplification o ...201728889914
epidemiology of danish aeromonas salmonicida subsp. salmonicida in fish farms using whole genome sequencing.furunculosis, a serious infection caused by the bacterium aeromonas salmonicida subsp. salmonicida is common in sea-reared rainbow trout production in denmark. developing an effective control strategy requires knowledge of the epidemiology, as well as the genomic and virulent variability of the danish a. salmonicida subsp. salmonicida isolates. to obtain this, the genomes of 101 a. salmonicida subsp. salmonicida, including 99 danish isolates, one scottish strain and the type strain ncimb 1102, w ...201729259599
vaccination of atlantic lumpfish (cyclopterus lumpus l.) at a low temperature leads to a low antibody response against aeromonas salmonicida.we present a study on the effect of water temperature on immunization of atlantic lumpfish. in total, 360 fish were vaccinated with either 50 μl of an oil-based injection vaccine (vax), with aeromonas salmonicida and vibrio salmonicida antigens, or pbs. fish were vaccinated at three different water temperatures, 5°c, 10°c and 15°c, and sorted into six groups (n = 60). lumpfish were weighed every 3 weeks after vaccination, sampled at 3, 6, 9 and 18 weeks post-immunization (wpi) and evaluated by m ...201729226986
aldh7a1 is a protein that protects atlantic salmon against aeromonas salmonicida at the early stages of infection.aldehyde dehydrogenases (aldhs) belong to a super-family of detoxifying proteins and perform a significant role in developing epithelial homeostasis, protecting cells from toxic aldehydes and drug resistance. however, the activity and function of these detoxifying proteins remain unknown, especially in fish. in our research, we aimed to study functions of aldehyde dehydrogenase 7a1 (aldh7a1) in atlantic salmon infected by aeromonas salmonicida. recombinant aldh7a1 (raldh7a1) was verified by sds- ...201728867386
plasmid composition in aeromonas salmonicida subsp. salmonicida 01-b526 unravels unsuspected type three secretion system loss patterns.aeromonas salmonicida subsp. salmonicida is a ubiquitous psychrophilic waterborne bacterium and a fish pathogen. the numerous mobile elements, especially insertion sequences (is), in its genome promote rearrangements that impact its phenotype. one of the main virulence factors of this bacterium, its type three secretion system (ttss), is affected by these rearrangements. in aeromonas salmonicida subsp. salmonicida most of the ttss genes are encoded in a single locus on a large plasmid called pas ...201728701230
study of mesophilic aeromonas salmonicida a527 strain sheds light on the species' lifestyles and taxonomic dilemma.the gram-negative bacterium aeromonas salmonicida contains five subspecies: salmonicida, smithia, achromogenes, masoucida and pectinolytica. pectinolytica is a mesophilic subspecies with the ability to thrive at a wide range of temperatures, including 37°c, while the four other subspecies are psychrophilic, restricted to lower temperatures. the psychrophilic subspecies are known to infect a wide range of fishes. however, there is no evidence of pathogenicity for the mesophilic subspecies pectino ...201729126137
evaluation of antibody response to an adjuvanted hapten-protein vaccine as a potential inhibitor of sexual maturation for farmed atlantic salmon.an experimental contraceptive vaccine was evaluated in atlantic salmon (salmo salar). a peptide derived from the beta subunit of luteinizing hormone (lh) was conjugated to two different carrier proteins, bovine serum albumin (bsa) and keyhole limpet hemocyanin (klh), and formulated with one of four immunostimulants in a water-in-oil emulsion. specific antibody responses to the peptide and each carrier protein were evaluated. while the antibody response to klh was stronger than the response to bs ...201728866277
biodegradation of benzo[a]pyrene by bacterial consortium isolated from mangrove sediment.benzo[a]pyrene is a high-molecular-weight polycyclic aromatic hydrocarbon highly recalcitrant in nature and thus harms the ecosystem and/or human health. therefore, its removal from the marine environment is crucial. this research focuses on benzo[a]pyrene degradation by using enriched bacterial isolates in consortium under saline conditions. bacterial isolates capable of using benzo[a]pyrene as sole source of carbon and energy were isolated from enriched mangrove sediment. these isolates were i ...201728281885
involvement of luxs in aeromonas salmonicida metabolism, virulence and infection in atlantic salmon (salmo salar l).quorum sensing is a bacterial density dependent communication system, which regarded to regulate co-operative behaviors of community and mediated by extracellular signal molecules named autoinducers (ai). among various signals, autoinducer-2 (ai-2) is believed to be the messengers inter species and produced by luxs. for aeromonas salmonicida (a. salmonicida), an opportunistic pathogen to many cold-water teleost, little information has been known about the function of ai-2 and luxs. therefore, ou ...201728279794
draft genome sequence of pseudomonas fluorescens ml11a, an endogenous strain from brook charr with antagonistic properties against aeromonas salmonicida subsp. salmonicida.pseudomonas fluorescens ml11a, isolated from brook charr, showed a strong in vitro inhibitory effect against aeromonas salmonicida subsp. salmonicida, a bacterial fish pathogen. its genome harbors gene clusters for siderophore and bacteriocin biosynthesis and shares 99% whole-genome identity with p. fluorescens a506, a biological control strain used in agriculture.201728254983
evaluation of the spoilage potential of bacteria isolated from chilled chicken in vitro and in situ.microorganisms play an important role in the spoilage of chilled chicken. in this study, a total of 53 isolates, belonging to 7 species of 3 genera, were isolated using a selective medium based on the capacity to spoil chicken juice. four isolates, namely aeromonas salmonicida 35, pseudomonas fluorescens h5, pseudomonas fragi h8 and serratia liquefaciens 17, were further characterized to assess their proteolytic activities in vitro using meat protein extracts and to evaluate their spoilage poten ...201728040161
aeromonas salmonicida type iii secretion system-effectors-mediated immune suppression in rainbow trout (oncorhynchus mykiss).aeromonas salmonicida subsp. salmonicida, the etiologic agent of furunculosis, is a major pathogen in aquaculture. together with other pathogens, it is characterized by the presence of a type 3 secretion system (t3ss). the t3ss is the main virulence mechanism of a. salmonicida. it is used by the bacterium to secrete and translocate several toxins and effector proteins into the host cell. some of these factors have a detrimental impact on the integrity of the cell cytoskeleton, likely contributin ...201727923746
characterization and mechanism of anti-aeromonas salmonicida activity of a marine probiotic strain, bacillus velezensis v4.the bacterium aeromonas salmonicida is the causative agent of furunculosis, a systemic, ubiquitous disease of fish in the salmon family, characterized by high mortality and morbidity. probiotics are a promising approach for prevention of furunculosis in aquaculture. a bacterial strain with anti-a. salmonicida properties, bacillus velezensis v4, was isolated and the mechanisms underlying these properties were investigated. anti-a. salmonicida compounds present in cell-free supernatant of v4 were ...201728074223
use of common carp (cyprinus carpio) and aeromonas salmonicida for detection of immunomodulatory effects of chemicals on fish.to develop a test for assessing the immunomodulatory effects of chemical pollutants on fish, we evaluated the effects of dexamethasone on the natural host-pathogen interaction between common carp (cyprinus carpio) and aeromonas salmonicida. carp were exposed to 1mgl(-1) dexamethasone for the entire experimental period. one week after the exposure test started, the exposed fish, as well as unexposed fish, were bath-infected with a. salmonicida. one hundred percent mortality was observed in bacter ...201728063699
assessment of hemolytic activity, enzyme production and bacteriocin characterization of bacillus subtilis lr1 isolated from the gastrointestinal tract of fish.in the present investigation, probiotic potential (antagonistic activity, enzyme production, hemolytic activity, biosafety, antibiotic sensitivity and bile tolerance level) of bacillus subtilis lr1 was evaluated. bacteriocin produced by the bacterial strain b. subtilis lr1 isolated from the gastrointestinal tract of labeo rohita was purified and characterized. the molecular weight of the purified bacteriocin was ~50 kda in 12 % native page and showed inhibitory activity against four fish pathoge ...201727590016
bacteriophage remediation of bacterial pathogens in aquaculture: a review of the technology.bacteriophages have been proposed as an alternative to antibiotic usage and several studies on their application in aquaculture have been reported. this review highlights progress to date on phage therapies for the following fish and shellfish diseases and associated pathogens: hemorrhagic septicemia (aeromonas hydrophila) in loaches, furunculosis (aeromonas salmonicida) in trout and salmon, edwardsiellosis (edwardsiella tarda) in eel, columnaris disease (flavobacterium columnare) in catfish, ra ...201726713223
identification of the ferric-acinetobactin outer membrane receptor in aeromonas salmonicida subsp. salmonicida and structure-activity relationships of synthetic acinetobactin analogues.aeromonas salmonicida subsp. salmonicida, the causative agent of furunculosis in several fish species, produces acinetobactin and amonabactin as siderophores. in a previous study, we chemically characterized these siderophores and proposed a biosynthetic pathway based on genetic analysis. however, the internalization mechanisms of ferric-acinetobactin and ferric-amonabactin remain largely unknown. in the present study, we demonstrate that the outer membrane protein fstb is the ferric-acinetobact ...201727936588
probiotic potential of autochthonous bacteria isolated from the gastrointestinal tract of four freshwater teleosts.in this study, a total of 121 bacterial strains were isolated from the gastrointestinal tract of four teleostean species, namely striped snakehead (channa striatus), striped dwarf catfish (mystus vittatus), orangefin labeo (labeo calbasu) and mrigal carp (cirrhinus mrigala), among which 8 isolates showed promising antibacterial activity against four potential fish pathogens, aeromonas hydrophila, aeromonas salmonicida, aeromonas sobria and pseudomonas fluorescens and were non-hemolytic. the isol ...201727557836
nocardiosis in freshwater reared chinook salmon (oncorhynchus tshawytscha).an investigation was conducted to identify the cause of mortalities in freshwater reared chinook salmon (oncorhynchus tshawytscha). mortalities occurred in juvenile salmon, at a salmon rearing facility in the south island of new zealand. the affected fish were from a pen inside the facility with no surrounding pens or other year classes affected.201728372487
proteomic analysis in kidneys of atlantic salmon infected with aeromonas salmonicida by itraq.aeromonas salmonicida is a major etiologic agent which induces furunculosis and is globally harmful in salmonid and turbot cultures, especially in atlantic salmon (salmo salar) farming. in order to improve knowledge of its poorly understood pathogenesis, we utilized high-throughput proteomics to display differentially expressed proteins in the kidney of atlantic salmon challenged with high and low infection dose of a. salmonicida at 7 and 14 days. in quantitative proteomic assays, isobaric tags ...201728235584
subunit vaccine candidates against aeromonas salmonicida in rainbow trout oncorhynchus mykiss.aeromonas salmonicida subsp. salmonicida is the etiological agent of furunculosis and a major fish health problem in salmonid aquaculture worldwide. injection vaccination with commercial mineral oil-adjuvanted bacterin vaccines has been partly successful in preventing the disease but in danish rainbow trout (oncorhynchus mykiss, walbaum) aquaculture furunculosis outbreaks still occur. in this study we tested the efficacy of experimental subunit vaccines against a. salmonicida infection in rainbo ...201728182704
phylogenetic analysis of the fish pathogen aeromonas salmonicida underlines the dichotomy between european and canadian strains for the salmonicida subspecies. 201728105757
multiple gene and transcript variants encoding trout c-polysaccharide binding proteins are differentially but strongly induced after infection with aeromonas salmonicida.two 'trout c-polysaccharide-binding proteins,' tcbp1 and -2, with relevance to early inflammatory events have been discovered in the last century. the present study characterises the respective cdna sequences from rainbow trout (oncorhynchus mykiss), including multiple tcbp1 transcript variants. these variants are generated either by the use of alternative splice sites or the exclusion of exons. the longest mrna isoform, tcbp1-1, encodes a 245-aa protein with a large signal peptide and a complem ...201727836722
infection routes of aeromonas salmonicida in rainbow trout monitored in vivo by real-time bioluminescence imaging.recent development of imaging tools has facilitated studies of pathogen infections in vivo in real time. this trend can be exemplified by advances in bioluminescence imaging (bli), an approach that helps to visualize dissemination of pathogens within the same animal over several time points. here, we employ bacterial bli for examining routes of entry and spread of aeromonas salmonicida susbp. salmonicida in rainbow trout. a virulent danish a. salmonicida strain was tagged with pakgfplux1, a dual ...201727307098
detection and quantification of aeromonas salmonicida in fish tissue by real-time pcr.furunculosis, a septicaemic infection caused by the bacterium aeromonas salmonicida subsp. salmonicida, currently causes problems in danish seawater rainbow trout production. detection has mainly been achieved by bacterial culture, but more rapid and sensitive methods are needed. a previously developed real-time pcr assay targeting the plasmid encoded aopp gene of a. salmonicida was, in parallel with culturing, used for the examination of five organs of 40 fish from danish freshwater and seawate ...201727193829
the effect of reducing agents on challenge of rainbow trout with aeromonas salmonicida. 201727291986
cold-water vibriosis. the current status of knowledge.the current review for the first time summarizes the findings of the 30 years of research on cold-water vibriosis (cwv). the diseased caused by aliivibrio salmonicida (earlier known as vibrio salmonicida) was for the first time described in 1986 and became one of the most important bacterial diseases in salmon aquaculture. the lack of appropriate vaccine hampered development of atlantic salmon aquaculture until the late 1980s when a novel vaccine allowed dramatic increase in the atlantic salmon ...201727072873
protection and antibody reactivity following vaccination of lumpfish (cyclopterus lumpus l.) against atypical aeromonas salmonicida.atypical aeromonas salmonicida is frequently associated with disease and mortality in farmed lumpfish (cyclopterus lumpus l). challenge experiments using different modes of exposure identified both high and low pathogenic isolates. intraperitoneal vaccination induced production of high levels of specific antibodies particularly in fish given multiple injections. the immune sera contained antibodies cross reactive with both high and low pathogenic isolates. sds-page and lc/msms analyses showed th ...201728344167
antimicrobial effect of the biotronic® top3 supplement and efficacy in protecting rainbow trout (oncorhynchus mykiss) from infection by aeromonas salmonicida subsp. salmonicida.demand for more environmentally friendly practices have led to the adoption of several feed supplements by the fish farming industry. in the present study, we investigated a commercially available formula that includes a mixture of three compounds: organic acids, a phytochemical and biomin® permeabilizing complex. this mixture demonstrated antimicrobial properties in vitro and was able to inhibit growth of multiple species of aquatic bacterial pathogens, including aeromonas salmonicida. bacteria ...201728343146
phylogeny and expression analysis of c-reactive protein (crp) and serum amyloid-p (sap) like genes reveal two distinct groups in fish.the acute phase response (apr) is an early innate immune function that is initiated by inflammatory signals, leading to the release of acute phase proteins to the bloodstream to re-establish homeostasis following microbial infection. in this study we analysed the atlantic salmon (salmo salar) whole-genome database and identified five c-reactive protein (crp)/serum amyloid p component (sap) like molecules namely crp/sap-1a, crp/sap-1b, crp/sap-1c, crp/sap-2 and crp/sap-3. these crp/sap genes form ...201728336487
effect of dietary β-glucan on growth, survival and regulation of immune processes in rainbow trout (oncorhynchus mykiss) infected by aeromonas salmonicida.the present study evaluated the effects of dietary β-glucan (0, 0.05%, 0.1%, and 0.2%) on growth performance after 42 days of feeding. thereafter, rainbow trout (oncorhynchus mykiss) were infected with aeromonas salmonicida, and survival rates as well as the regulating processes of stress- and immune-related factors were analyzed. in general, higher dietary β-glucan levels obviously improved specific growth rate (sgr), weight gain (wg) and feed efficiency (fe) (p ≤ 0.05). survival rates in β-glu ...201728286256
pharmacokinetic model of florfenicol in turbot (scophthalmus maximus): establishment of optimal dosage and administration in medicated feed.the pharmacokinetics of florfenicol (ff) in turbot (scophthalmus maximus) was studied after single intravenous (10 mg kg(-1) ) and oral (100 mg kg(-1) ) administration. the plasma concentration-time data of florfenicol were described by an open one-compartment model. the elimination half-life (t1/2 ) was estimated to be 21.0 h, and the total body clearance, cl, was determined as 0.028 l kg h(-1) . the apparent volume distribution (vd ) was calculated to be 0.86 l kg(-1) and the mean residence ti ...201727502011
positive correlation between aeromonas salmonicida vaccine antigen concentration and protection in vaccinated rainbow trout oncorhynchus mykiss evaluated by a tail fin infection model.rainbow trout, oncorhynchus mykiss (walbaum), are able to raise a protective immune response against aeromonas salmonicida subsp. salmonicida (as) following injection vaccination with commercial vaccines containing formalin-killed bacteria, but the protection is often suboptimal under danish mariculture conditions. we elucidated whether protection can be improved by increasing the concentration of antigen (formalin-killed bacteria) in the vaccine. rainbow trout juveniles were vaccinated by intra ...201727593619
st2 from rainbow trout quenches tlr signalling, localises at the nuclear membrane and allows the nuclear translocation of myd88.the mammalian interleukin 1 receptor-like 1 receptor (il1rl1), commonly known as st2, is thought to downregulate tlr signalling by sequestering the signalling adapter myd88 (myeloid differentiation primary response protein 88). st2 sequences are known in several fish species, but none of them have functionally been examined. we characterised st2 from rainbow trout (oncorhynchus mykiss) and the structure of its encoding gene. the primary sequence of st2 is only weakly conserved from fish to human ...201727776995
inducers of salmon innate immunity: an in vitro and in vivo approach.maintaining fish health is one of the most important aims in aquaculture. prevention of fish diseases therefore is crucial and can be achieved by various different strategies, including most often a combination of different methods such as optimal feed and fish density, as well as strengthening the immune system. understanding the fish innate immune system and developing methods to activate it, in an effort to prevent infections in the first place, has been a goal in recent years. in this study ...201829108970
the complete and fully assembled genome sequence of aeromonas salmonicida subsp. pectinolytica and its comparative analysis with other aeromonas species: investigation of the mobilome in environmental and pathogenic strains.due to the predominant usage of short-read sequencing to date, most bacterial genome sequences reported in the last years remain at the draft level. this precludes certain types of analyses, such as the in-depth analysis of genome plasticity.201829304740
in vivo selection of a multidrug-resistant aeromonas salmonicida during medicinal leech therapy.we report the selection in a 15-year-old boy of a multidrug-resistant, extended-spectrum β-lactamase (esbl)-producing aeromonas salmonicida after medicinal leech therapy that required an antibiotic prophylaxis based on piperacillin/tazobactam and cotrimoxazole. whole genome sequencing of the strain indeed revealed 13 antibiotic resistance genes, including the esbl ctx-m-3 and the unusual β-lactamase sco-1.201829204282
first isolation of vibrio tapetis and an atypical strain of aeromonas salmonicida from skin ulcerations in common dab (limanda limanda) in the north sea.skin ulcerations rank amongst the most prevalent lesions affecting wild common dab (limanda limanda) with an increase in prevalence of up to 3.5% in the belgian part of the north sea. a complex aetiology of these ulcerations is suspected, and many questions remain on the exact factors contributing to these lesions. to construct the aetiological spectrum of skin ulcerations in flatfish, a one-day monitoring campaign was undertaken in the north sea. fifteen fish presented with one or more ulcerati ...201829148593
interplay between daily rhythmic serum-mediated bacterial killing activity and immune defence factors in rainbow trout (oncorhynchus mykiss).circadian rhythm is emerging as an important regulator of immune functions. however, there is a paucity of information on the influence of this biological phenomenon in the antimicrobial factors in teleost fish. this study investigated the dynamics and interplay of serum-mediated bacterial killing activity and immune defence factors throughout the light:dark (ld) cycle in rainbow trout (oncorhynchus mykiss). the juvenile fish came from two different emergence time fractions (i.e., late and early ...201829146445
identification of a moronecidin-like antimicrobial peptide in the venomous fish pterois volitans: functional and structural study of pteroicidin-α.the present study characterizes for the first time an antimicrobial peptide in lionfish (pterois volitans), a venomous fish. using a peptidomic approach, we identified a mature piscidin in lionfish and called it pteroicidin-α. we detected an amidated form (pteroicidin-α- conh2) and a non-amidated form (pteroicidin-α-cooh), and then performed their functional and structural study. interestingly, the two peptides displayed different antibacterial and hemolytic activity levels. pteroicidin-α-conh2 ...201829108968
genomic and phenotypic characterization of an atypical aeromonas salmonicida strain isolated from a lumpfish and producing unusual granular structures.aeromonas salmonicida strains are roughly classified into two categories, typical and atypical strains. the latter mainly regroup isolates that present unusual phenotypes or hosts, comparatively to the typical strains that belong to the salmonicida subspecies. this study focuses on an uncharacterized atypical strain, m18076-11, isolated from lumpfish (cyclopterus lumpus) and not part of the four recognized aeromonas salmonicida subspecies. this isolate presents an unreported phenotype in the a.  ...201829315617
characterization and functional assessment of the nlrc3-like molecule of the goldfish (carassius auratus l.).the nlrc3-like (nlrc3l) molecule from the goldfish transcriptome database was identified and characterized. quantitative gene expression analysis revealed the highest mrna levels of nlrc3l were in the spleen and intestine, with lower mrna levels observed in muscle and liver. goldfish nlrc3l was differentially expressed in goldfish immune cell populations with highest mrna levels measured in pbls and macrophages. we generated a recombinant form of the molecule (rgfnlrc3l) and an anti-ct-nlrc3l ig ...201828988993
Refresh
firstprev
...1112131415161718
nextlast
Displaying items 1701 - 1755 of 1755