Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| evaluation of the spoilage potential of bacteria isolated from chilled chicken in vitro and in situ. | microorganisms play an important role in the spoilage of chilled chicken. in this study, a total of 53 isolates, belonging to 7 species of 3 genera, were isolated using a selective medium based on the capacity to spoil chicken juice. four isolates, namely aeromonas salmonicida 35, pseudomonas fluorescens h5, pseudomonas fragi h8 and serratia liquefaciens 17, were further characterized to assess their proteolytic activities in vitro using meat protein extracts and to evaluate their spoilage poten ... | 2017 | 28040161 |
| use of common carp (cyprinus carpio) and aeromonas salmonicida for detection of immunomodulatory effects of chemicals on fish. | to develop a test for assessing the immunomodulatory effects of chemical pollutants on fish, we evaluated the effects of dexamethasone on the natural host-pathogen interaction between common carp (cyprinus carpio) and aeromonas salmonicida. carp were exposed to 1mgl(-1) dexamethasone for the entire experimental period. one week after the exposure test started, the exposed fish, as well as unexposed fish, were bath-infected with a. salmonicida. one hundred percent mortality was observed in bacter ... | 2017 | 28063699 |
| characterization and mechanism of anti-aeromonas salmonicida activity of a marine probiotic strain, bacillus velezensis v4. | the bacterium aeromonas salmonicida is the causative agent of furunculosis, a systemic, ubiquitous disease of fish in the salmon family, characterized by high mortality and morbidity. probiotics are a promising approach for prevention of furunculosis in aquaculture. a bacterial strain with anti-a. salmonicida properties, bacillus velezensis v4, was isolated and the mechanisms underlying these properties were investigated. anti-a. salmonicida compounds present in cell-free supernatant of v4 were ... | 2017 | 28074223 |
| phylogenetic analysis of the fish pathogen aeromonas salmonicida underlines the dichotomy between european and canadian strains for the salmonicida subspecies. | 2017 | 28105757 | |
| subunit vaccine candidates against aeromonas salmonicida in rainbow trout oncorhynchus mykiss. | aeromonas salmonicida subsp. salmonicida is the etiological agent of furunculosis and a major fish health problem in salmonid aquaculture worldwide. injection vaccination with commercial mineral oil-adjuvanted bacterin vaccines has been partly successful in preventing the disease but in danish rainbow trout (oncorhynchus mykiss, walbaum) aquaculture furunculosis outbreaks still occur. in this study we tested the efficacy of experimental subunit vaccines against a. salmonicida infection in rainbo ... | 2017 | 28182704 |
| proteomic analysis in kidneys of atlantic salmon infected with aeromonas salmonicida by itraq. | aeromonas salmonicida is a major etiologic agent which induces furunculosis and is globally harmful in salmonid and turbot cultures, especially in atlantic salmon (salmo salar) farming. in order to improve knowledge of its poorly understood pathogenesis, we utilized high-throughput proteomics to display differentially expressed proteins in the kidney of atlantic salmon challenged with high and low infection dose of a. salmonicida at 7 and 14 days. in quantitative proteomic assays, isobaric tags ... | 2017 | 28235584 |
| draft genome sequence of pseudomonas fluorescens ml11a, an endogenous strain from brook charr with antagonistic properties against aeromonas salmonicida subsp. salmonicida. | pseudomonas fluorescens ml11a, isolated from brook charr, showed a strong in vitro inhibitory effect against aeromonas salmonicida subsp. salmonicida, a bacterial fish pathogen. its genome harbors gene clusters for siderophore and bacteriocin biosynthesis and shares 99% whole-genome identity with p. fluorescens a506, a biological control strain used in agriculture. | 2017 | 28254983 |
| involvement of luxs in aeromonas salmonicida metabolism, virulence and infection in atlantic salmon (salmo salar l). | quorum sensing is a bacterial density dependent communication system, which regarded to regulate co-operative behaviors of community and mediated by extracellular signal molecules named autoinducers (ai). among various signals, autoinducer-2 (ai-2) is believed to be the messengers inter species and produced by luxs. for aeromonas salmonicida (a. salmonicida), an opportunistic pathogen to many cold-water teleost, little information has been known about the function of ai-2 and luxs. therefore, ou ... | 2017 | 28279794 |
| biodegradation of benzo[a]pyrene by bacterial consortium isolated from mangrove sediment. | benzo[a]pyrene is a high-molecular-weight polycyclic aromatic hydrocarbon highly recalcitrant in nature and thus harms the ecosystem and/or human health. therefore, its removal from the marine environment is crucial. this research focuses on benzo[a]pyrene degradation by using enriched bacterial isolates in consortium under saline conditions. bacterial isolates capable of using benzo[a]pyrene as sole source of carbon and energy were isolated from enriched mangrove sediment. these isolates were i ... | 2017 | 28281885 |
| effect of dietary β-glucan on growth, survival and regulation of immune processes in rainbow trout (oncorhynchus mykiss) infected by aeromonas salmonicida. | the present study evaluated the effects of dietary β-glucan (0, 0.05%, 0.1%, and 0.2%) on growth performance after 42 days of feeding. thereafter, rainbow trout (oncorhynchus mykiss) were infected with aeromonas salmonicida, and survival rates as well as the regulating processes of stress- and immune-related factors were analyzed. in general, higher dietary β-glucan levels obviously improved specific growth rate (sgr), weight gain (wg) and feed efficiency (fe) (p ≤ 0.05). survival rates in β-glu ... | 2017 | 28286256 |
| phylogeny and expression analysis of c-reactive protein (crp) and serum amyloid-p (sap) like genes reveal two distinct groups in fish. | the acute phase response (apr) is an early innate immune function that is initiated by inflammatory signals, leading to the release of acute phase proteins to the bloodstream to re-establish homeostasis following microbial infection. in this study we analysed the atlantic salmon (salmo salar) whole-genome database and identified five c-reactive protein (crp)/serum amyloid p component (sap) like molecules namely crp/sap-1a, crp/sap-1b, crp/sap-1c, crp/sap-2 and crp/sap-3. these crp/sap genes form ... | 2017 | 28336487 |
| antimicrobial effect of the biotronic® top3 supplement and efficacy in protecting rainbow trout (oncorhynchus mykiss) from infection by aeromonas salmonicida subsp. salmonicida. | demand for more environmentally friendly practices have led to the adoption of several feed supplements by the fish farming industry. in the present study, we investigated a commercially available formula that includes a mixture of three compounds: organic acids, a phytochemical and biomin® permeabilizing complex. this mixture demonstrated antimicrobial properties in vitro and was able to inhibit growth of multiple species of aquatic bacterial pathogens, including aeromonas salmonicida. bacteria ... | 2017 | 28343146 |
| protection and antibody reactivity following vaccination of lumpfish (cyclopterus lumpus l.) against atypical aeromonas salmonicida. | atypical aeromonas salmonicida is frequently associated with disease and mortality in farmed lumpfish (cyclopterus lumpus l). challenge experiments using different modes of exposure identified both high and low pathogenic isolates. intraperitoneal vaccination induced production of high levels of specific antibodies particularly in fish given multiple injections. the immune sera contained antibodies cross reactive with both high and low pathogenic isolates. sds-page and lc/msms analyses showed th ... | 2017 | 28344167 |
| antimicrobial activities of secondary metabolites and phylogenetic study of sponge endosymbiotic bacteria, bacillus sp. at agatti island, lakshadweep archipelago. | twenty-one species of sponges were recorded under the class of demospongiae and calcareous sponges of which 19 species were new to agatti reef. a total of 113 sponge endosymbiotic bacterial strains were isolated from twenty-one species of sponges and screened for antimicrobial activity. five bacterial strains of sponge endosymbiotic bacteria (seb) namely seb32, seb33, seb36, seb43 and seb51 showed antimicrobial activity against virulent marine fish pathogens such as vibrio alginolyticus, vibrio ... | 2016 | 28352539 |
| echinoderm immunology: bacterial clearance by the sea urchin strongylocentrotus purpuratus. | characteristics of bacterial clearance were investigated in the purple sea urchin, strongylocentrotus purpuratus (echinodermata: echinoidea). primary clearance kinetics were determined for three bacteria, a marine gram negative motile rod, a marine gram positive non-motile rod, and a gram negative freshwater fish pathogen, aeromonas salmonicida. clearance kinetics differed for each of the three bacteria. secondary clearance rates were not significantly different from primary clearance rates for ... | 1983 | 28368229 |
| nocardiosis in freshwater reared chinook salmon (oncorhynchus tshawytscha). | an investigation was conducted to identify the cause of mortalities in freshwater reared chinook salmon (oncorhynchus tshawytscha). mortalities occurred in juvenile salmon, at a salmon rearing facility in the south island of new zealand. the affected fish were from a pen inside the facility with no surrounding pens or other year classes affected. | 2017 | 28372487 |
| transcriptional changes in innate immunity genes in head kidneys from aeromonas salmonicida-challenged rainbow trout fed a mixture of polycyclic aromatic hydrocarbons. | we previously observed that exposure to a complex mixture of high molecular weight polycyclic aromatic hydrocarbons (pahs) increased sensitivity of rainbow trout (oncorhynchus mykiss) to subsequent challenge with aeromonas salmonicida, the causative agent of furunculosis. in this study, we evaluate potential mechanisms associated with disease susceptibility from combined environmental factors of dietary pah exposure and pathogen challenge. rainbow trout were fed a mixture of ten high molecular w ... | 2017 | 28407501 |
| the biofilteration ability of oysters (crassostrea gigas) to reduce aeromonas salmonicida in salmon culture. | pathogen contamination in the environment is inevitable with the rapid development of intensive aquaculture. therefore, alternative ecofriendly biological strategies to control pathogenic bacteria are required. however, our aim was to investigate the ability of oysters (crassostrea gigas) to filter the important opportunistic pathogen, aeromonas salmonicida (strain c4), using a green fluorescent protein tag (gfp) in the atlantic salmon (salmo salar) farming wastewater. hence, a. salmonicida remo ... | 2017 | 28474104 |
| the protective effect of humic-rich substances on atypical aeromonas salmonicida subsp. salmonicida infection in common carp (cyprinus carpio l.). | when challenged with atypical aeromonas salmonicida subsp. salmonicida, exposure of the common carp (cyprinus carpio l.) to different humic-rich compounds resulted in a significant reduction in infection rates. specifically, in fish exposed to (i) humic-rich water and sludge from a recirculating system, (ii) a synthetic humic acid, and (iii) a leonardite-derived humic-rich extract, infection rates were reduced to 14.9%, 17.0% and 18.8%, respectively, as compared to a 46.8% infection rate in the ... | 2017 | 28493490 |
| differential effects of exposure to parasites and bacteria on stress response in turbot scophthalmus maximus simultaneously stressed by low water depth. | the stress response of turbot scophthalmus maximus was evaluated in fish maintained 8 days under different water depths, normal (nwd, 30 cm depth, total water volume 40 l) or low (lwd, 5 cm depth, total water volume 10 l), in the additional presence of infection-infestation of two pathogens of this species. this was caused by intraperitoneal injection of sublethal doses of the bacterium aeromonas salmonicida subsp. salmonicida or the parasite philasterides dicentrarchi (ciliophora:scuticociliati ... | 2017 | 28516502 |
| in vitro effects of italian lavandula multifida l. leaf extracts on gilthead seabream (sparus aurata) leucocytes and saf-1 cells. | lavandula multifida is very appreciated by pharmaceutical and cosmetic industries. in italy is only found in calabria and sicily and, at present, urge its valorization due to its high extinction and genetic erosion risks. possible applications of l. multifida extracts as immunostimulant in fish aquaculture were assayed by using gilthead seabream (sparus aurata) as a marine fish model, due to its importance in fish aquaculture. the in vitro effects of both aqueous and ethanolic leaf extracts obta ... | 2017 | 28522420 |
| aeromonas salmonicida growth in response to atlantic salmon mucins differs between epithelial sites, is governed by sialylated and n-acetylhexosamine-containing o-glycans, and is affected by ca(2). | aeromonas salmonicida causes furunculosis in salmonids and is a threat to atlantic salmon aquaculture. the epithelial surfaces that the pathogen colonizes are covered by a mucus layer predominantly comprised of secreted mucins. by using mass spectrometry to identify mucin glycan structures with and without enzymatic removal of glycan residues, coupled to measurements of bacterial growth, we show here that the complex atlantic salmon intestinal mucin glycans enhance a. salmonicida growth, whereas ... | 2017 | 28533470 |
| studies on the antibody response and side effects after intramuscular and intraperitoneal injection of atlantic lumpfish (cyclopterus lumpus l.) with different oil-based vaccines. | atlantic lumpfish (cyclopterus lumpus l.) is used as a biological delousing agent for sea lice (lepeophtheirus salmonis k.) infestations in norwegian aquaculture. here, we present a study on the antibody response and vaccine side effects after intramuscular and intraperitoneal injection of lumpfish with two vaccines. both vaccines contained bacterial antigens from atypical aeromonas salmonicida a-layer types v and vi, vibrio anguillarum serotype o1 and moritella viscosa sp., but one vaccine cont ... | 2017 | 28548686 |
| phenotype of aeromonas salmonicida sp. salmonicida cyclic adenosine 3',5'-monophosphate receptor protein (crp) mutants and its virulence in rainbow trout (oncorhynchus mykiss). | precise deletion of genes related to virulence can be used as a strategy to produce attenuated bacterial vaccines. here, we study the deletion of the cyclic-3',5'-adenosine monophosphate (camp) receptor protein (crp) in aeromonas salmonicida, the aetiologic agent of furunculosis in marine and freshwater fish. the crp protein is a conserved global regulator, controlling physiology processes, like sugar utilization. deletion of the crp gene has been utilized in live attenuated vaccines for mammals ... | 2017 | 28548689 |
| characterization of the cultivable gut microflora in wild-caught mediterranean fish species. | microflora of the gastrointestinal tract plays important roles in food digestion, nutrient absorption and in host defense against ingested pathogens. several studies have focused on the microflora of farmed fishes, but the gut flora of wild fishes remains poorly characterized. the aim of this work was to provide an overview of the bacteria colonizing the gut of wild-caught fishes and to determine whether some bacterial species can be pathogenic. | 2017 | 28553195 |
| aeromonas salmonicida bacteremia associated with chronic well water consumption in a patient with diabetes. | aeromonas salmonicida is associated with superficial skin infections in fish. its virulence factors allow colonization of water including surface water such as salt water, beaches, and fresh water wells. moreover, it is possible for immunocompromised patients to develop invasive disease after chronic exposure to aeromonas spp. through contaminated water. while there are reports of aeromonas spp. bacteremia following water ingestion, there have been no reports of a. salmonicida bacteremia from wa ... | 2017 | 28584462 |
| species distribution and prevalence of putative virulence factors in mesophilic aeromonas spp. isolated from fresh retail sushi. | aeromonas spp. are ubiquitous bacteria that have received increasing attention as human pathogens because of their widespread occurrence in food, especially seafood and vegetables. the aim of this work was to assess the species identity and phylogenetic relationship of 118 aeromonas strains isolated from fresh retail sushi from three producers, and to characterize the isolates with respect to genetic and phenotypic virulence factors. we also evaluate the potential hazard associated with their pr ... | 2017 | 28596762 |
| analysis of the genome and mobilome of a dissimilatory arsenate reducing aeromonas sp. o23a reveals multiple mechanisms for heavy metal resistance and metabolism. | aeromonas spp. are among the most ubiquitous microorganisms, as they have been isolated from different environmental niches including waters, soil, as well as wounds and digestive tracts of poikilothermic animals and humans. although much attention has been paid to the pathogenicity of aeromonads, the role of these bacteria in environmentally important processes, such as transformation of heavy metals, remains to be discovered. therefore, the aim of this study was a detailed genomic characteriza ... | 2017 | 28611742 |
| analysis of adipose tissue immune gene expression after vaccination of rainbow trout with adjuvanted bacterins reveals an association with side effects. | most existing fish vaccines are presented in the form of oil-based emulsions delivered by intraperitoneal injection. whilst very effective they are frequently associated with inflammatory responses that can result in clinically significant side-effects often involving the adipose tissue that is in direct contact with the vaccine. to explore the potential of immune gene expression changes in the adipose tissue of fish to be markers of vaccination efficacy or development of side-effects we have st ... | 2017 | 28622574 |
| novel plasmid-mediated colistin resistance gene mcr-3 in escherichia coli. | the mobile colistin resistance gene mcr-1 has attracted global attention, as it heralds the breach of polymyxins, one of the last-resort antibiotics for the treatment of severe clinical infections caused by multidrug-resistant gram-negative bacteria. to date, six slightly different variants of mcr-1, and a second mobile colistin resistance gene, mcr-2, have been reported or annotated in the genbank database. here, we characterized a third mobile colistin resistance gene, mcr-3 the gene coexisted ... | 2017 | 28655818 |
| plasmid composition in aeromonas salmonicida subsp. salmonicida 01-b526 unravels unsuspected type three secretion system loss patterns. | aeromonas salmonicida subsp. salmonicida is a ubiquitous psychrophilic waterborne bacterium and a fish pathogen. the numerous mobile elements, especially insertion sequences (is), in its genome promote rearrangements that impact its phenotype. one of the main virulence factors of this bacterium, its type three secretion system (ttss), is affected by these rearrangements. in aeromonas salmonicida subsp. salmonicida most of the ttss genes are encoded in a single locus on a large plasmid called pas ... | 2017 | 28701230 |
| a historical review of the key bacterial and viral pathogens of scottish wild fish. | thousands of scottish wild fish were screened for pathogens by marine scotland science. a systematic review of published and unpublished data on six key pathogens (renibacterium salmoninarum, aeromonas salmonicida, ipnv, isav, sav and vhsv) found in scottish wild and farmed fish was undertaken. despite many reported cases in farmed fish, there was a limited number of positive samples from scottish wild fish, however, there was evidence for interactions between wild and farmed fish. a slightly el ... | 2017 | 28718925 |
| alternatives to mineral oil adjuvants in vaccines against aeromonas salmonicida subsp. salmonicida in rainbow trout offer reductions in adverse effects. | in an effort to reduce the frequency and severity of adverse reactions seen from the use of mineral oil adjuvants in salmonid fish, the effects of two alternative adjuvants were assessed, focusing on the induction of adverse effects as well as protection. using rainbow trout (oncorhynchus mykiss) as recipients, injection vaccines based on formalin-inactivated aeromonas salmonicida subspecies salmonicida were formulated with cpg oligodeoxynucleotides, the liposomal cationic adjuvant formulation 0 ... | 2017 | 28724973 |
| comparative pathogenicity of vibrio spp., photobacterium damselae ssp. damselae and five isolates of aeromonas salmonicida ssp. achromogenes in juvenile atlantic halibut (hippoglossus hippoglossus). | juvenile atlantic halibut (~100 mg, hippoglossus hippoglossus) were exposed to vibrio proteolyticus, a vibrio spp. isolate, photobacterium damselae ssp. damselae and five different isolates of aeromonas salmonicida ssp. achromogenes via an hour-long bath immersion to ascertain their variation in pathogenicity to this fish species. results were analysed using kaplan-meier survival analysis. analysis of the data from challenges using a. salmonicida ssp. achromogenes revealed three survival values ... | 2017 | 28731211 |
| in vitro assessment of the antimicrobial activity of silver and zinc oxide nanoparticles against fish pathogens. | antibiotic resistance is a global issue that threatens public health. the excessive use of antibiotics contributes to this problem as the genes of antibiotic resistance can be transferred between the bacteria in humans, animals and aquatic organisms. metallic nanoparticles could serve as future substitutes for some conventional antibiotics because of their antimicrobial activity. the aim of this study was to evaluate the antimicrobial effects of silver and zinc oxide nanoparticles against major ... | 2017 | 28732514 |
| characterization and diversity of phages infecting aeromonas salmonicida subsp. salmonicida. | phages infecting aeromonas salmonicida subsp. salmonicida, the causative agent of the fish disease furunculosis, have been isolated for decades but very few of them have been characterized. here, the host range of 12 virulent phages, including three isolated in the present study, was evaluated against a panel of 65 a. salmonicida isolates, including representatives of the psychrophilic subspecies salmonicida, smithia, masoucida, and the mesophilic subspecies pectinolytica. this bacterial set als ... | 2017 | 28765570 |
| chromosome-mediated mcr-3 variants in aeromonas veronii from chicken meat. | two adjacent colistin resistance gene variants, termed mcr-3.2 and mcr-3-like, were identified in the chromosome of an aeromonas veronii isolated from retail chicken meat. the variants showed 95.20% and 84.19% nucleotide sequence identity, respectively, to mcr-3 from porcine escherichia coli functional cloning indicated that only mcr-3.2 conferred polymyxin resistance in both e. coli and aeromonas salmonicida the mcr-3.2-mcr-3-like segment was also observed in other aeromonas species, including ... | 2017 | 28848017 |
| evaluation of antibody response to an adjuvanted hapten-protein vaccine as a potential inhibitor of sexual maturation for farmed atlantic salmon. | an experimental contraceptive vaccine was evaluated in atlantic salmon (salmo salar). a peptide derived from the beta subunit of luteinizing hormone (lh) was conjugated to two different carrier proteins, bovine serum albumin (bsa) and keyhole limpet hemocyanin (klh), and formulated with one of four immunostimulants in a water-in-oil emulsion. specific antibody responses to the peptide and each carrier protein were evaluated. while the antibody response to klh was stronger than the response to bs ... | 2017 | 28866277 |
| aldh7a1 is a protein that protects atlantic salmon against aeromonas salmonicida at the early stages of infection. | aldehyde dehydrogenases (aldhs) belong to a super-family of detoxifying proteins and perform a significant role in developing epithelial homeostasis, protecting cells from toxic aldehydes and drug resistance. however, the activity and function of these detoxifying proteins remain unknown, especially in fish. in our research, we aimed to study functions of aldehyde dehydrogenase 7a1 (aldh7a1) in atlantic salmon infected by aeromonas salmonicida. recombinant aldh7a1 (raldh7a1) was verified by sds- ... | 2017 | 28867386 |
| infections of edwardsiella tarda among brook trout in quebec. | acute bacterial septicemia is commonly diagnosed in brook trout salvelinus fontinalis of quebec, canada. the agents most commonly isolated include aeromonas salmonicida (furunculosis), aeromonas hydrophila (motile aeromonad septicemia), and pseudomonas species. septicemia in brook trout caused by the gram-negative bacterium edwardsiella tarda was diagnosed for the first time in the province of quebec from two different fish farms producing stock for fee fishing establishments. affected fish disp ... | 2000 | 28880779 |
| vaccination against furunculosis in arctic char: efficacy of a commercial vaccine. | arctic char salvelinus alpinus are susceptible to furunculosis, a serious bacterial disease that affects the productivity of salmonid farms around the world. the objective of this laboratory study was to evaluate the efficacy of a commercially available vaccine against furunculosis in two strains of arctic char. labrador and nauyuk char were injected with aqua health furogen 2 or 0.9% nacl. at 87 and 108 d postvaccination, fish were challenged with aeromonas salmonicida by immersion. in both cha ... | 2002 | 28880796 |
| microplastics as a vector for the transport of the bacterial fish pathogen species aeromonas salmonicida. | microplastics is widespread in the marine environment where it can cause numerous negative effects. it can provide space for the growth of organisms and serves as a vector for the long distance transfer of marine microorganisms. in this study, we examined the sea surface concentrations of microplastics in the north adriatic and characterized bacterial communities living on the microplastics. dna from microplastics particles was isolated by three different methods, followed by pcr amplification o ... | 2017 | 28889914 |
| draft genome sequence of the type strain aeromonas salmonicida subsp. salmonicida atcc 33658. | here, we report the draft genome sequence of the type strain aeromonas salmonicida subsp. salmonicida atcc 33658 isolated from salmo salar the size of the genome is 4,728,143 bp with a g+c content of 58.5%. the a. salmonicida subsp. salmonicida atcc 33658 genome lacks essential virulence genes that were likely lost during genomic rearrangements. | 2017 | 28982999 |
| characterization and functional assessment of the nlrc3-like molecule of the goldfish (carassius auratus l.). | the nlrc3-like (nlrc3l) molecule from the goldfish transcriptome database was identified and characterized. quantitative gene expression analysis revealed the highest mrna levels of nlrc3l were in the spleen and intestine, with lower mrna levels observed in muscle and liver. goldfish nlrc3l was differentially expressed in goldfish immune cell populations with highest mrna levels measured in pbls and macrophages. we generated a recombinant form of the molecule (rgfnlrc3l) and an anti-ct-nlrc3l ig ... | 2018 | 28988993 |
| identification of a moronecidin-like antimicrobial peptide in the venomous fish pterois volitans: functional and structural study of pteroicidin-α. | the present study characterizes for the first time an antimicrobial peptide in lionfish (pterois volitans), a venomous fish. using a peptidomic approach, we identified a mature piscidin in lionfish and called it pteroicidin-α. we detected an amidated form (pteroicidin-α- conh2) and a non-amidated form (pteroicidin-α-cooh), and then performed their functional and structural study. interestingly, the two peptides displayed different antibacterial and hemolytic activity levels. pteroicidin-α-conh2 ... | 2018 | 29108968 |
| inducers of salmon innate immunity: an in vitro and in vivo approach. | maintaining fish health is one of the most important aims in aquaculture. prevention of fish diseases therefore is crucial and can be achieved by various different strategies, including most often a combination of different methods such as optimal feed and fish density, as well as strengthening the immune system. understanding the fish innate immune system and developing methods to activate it, in an effort to prevent infections in the first place, has been a goal in recent years. in this study ... | 2018 | 29108970 |
| study of mesophilic aeromonas salmonicida a527 strain sheds light on the species' lifestyles and taxonomic dilemma. | the gram-negative bacterium aeromonas salmonicida contains five subspecies: salmonicida, smithia, achromogenes, masoucida and pectinolytica. pectinolytica is a mesophilic subspecies with the ability to thrive at a wide range of temperatures, including 37°c, while the four other subspecies are psychrophilic, restricted to lower temperatures. the psychrophilic subspecies are known to infect a wide range of fishes. however, there is no evidence of pathogenicity for the mesophilic subspecies pectino ... | 2017 | 29126137 |
| interplay between daily rhythmic serum-mediated bacterial killing activity and immune defence factors in rainbow trout (oncorhynchus mykiss). | circadian rhythm is emerging as an important regulator of immune functions. however, there is a paucity of information on the influence of this biological phenomenon in the antimicrobial factors in teleost fish. this study investigated the dynamics and interplay of serum-mediated bacterial killing activity and immune defence factors throughout the light:dark (ld) cycle in rainbow trout (oncorhynchus mykiss). the juvenile fish came from two different emergence time fractions (i.e., late and early ... | 2018 | 29146445 |
| first isolation of vibrio tapetis and an atypical strain of aeromonas salmonicida from skin ulcerations in common dab (limanda limanda) in the north sea. | skin ulcerations rank amongst the most prevalent lesions affecting wild common dab (limanda limanda) with an increase in prevalence of up to 3.5% in the belgian part of the north sea. a complex aetiology of these ulcerations is suspected, and many questions remain on the exact factors contributing to these lesions. to construct the aetiological spectrum of skin ulcerations in flatfish, a one-day monitoring campaign was undertaken in the north sea. fifteen fish presented with one or more ulcerati ... | 2018 | 29148593 |
| in vivo selection of a multidrug-resistant aeromonas salmonicida during medicinal leech therapy. | we report the selection in a 15-year-old boy of a multidrug-resistant, extended-spectrum β-lactamase (esbl)-producing aeromonas salmonicida after medicinal leech therapy that required an antibiotic prophylaxis based on piperacillin/tazobactam and cotrimoxazole. whole genome sequencing of the strain indeed revealed 13 antibiotic resistance genes, including the esbl ctx-m-3 and the unusual β-lactamase sco-1. | 2018 | 29204282 |
| vaccination of atlantic lumpfish (cyclopterus lumpus l.) at a low temperature leads to a low antibody response against aeromonas salmonicida. | we present a study on the effect of water temperature on immunization of atlantic lumpfish. in total, 360 fish were vaccinated with either 50 μl of an oil-based injection vaccine (vax), with aeromonas salmonicida and vibrio salmonicida antigens, or pbs. fish were vaccinated at three different water temperatures, 5°c, 10°c and 15°c, and sorted into six groups (n = 60). lumpfish were weighed every 3 weeks after vaccination, sampled at 3, 6, 9 and 18 weeks post-immunization (wpi) and evaluated by m ... | 2017 | 29226986 |
| epidemiology of danish aeromonas salmonicida subsp. salmonicida in fish farms using whole genome sequencing. | furunculosis, a serious infection caused by the bacterium aeromonas salmonicida subsp. salmonicida is common in sea-reared rainbow trout production in denmark. developing an effective control strategy requires knowledge of the epidemiology, as well as the genomic and virulent variability of the danish a. salmonicida subsp. salmonicida isolates. to obtain this, the genomes of 101 a. salmonicida subsp. salmonicida, including 99 danish isolates, one scottish strain and the type strain ncimb 1102, w ... | 2017 | 29259599 |
| the complete and fully assembled genome sequence of aeromonas salmonicida subsp. pectinolytica and its comparative analysis with other aeromonas species: investigation of the mobilome in environmental and pathogenic strains. | due to the predominant usage of short-read sequencing to date, most bacterial genome sequences reported in the last years remain at the draft level. this precludes certain types of analyses, such as the in-depth analysis of genome plasticity. | 2018 | 29304740 |
| genomic and phenotypic characterization of an atypical aeromonas salmonicida strain isolated from a lumpfish and producing unusual granular structures. | aeromonas salmonicida strains are roughly classified into two categories, typical and atypical strains. the latter mainly regroup isolates that present unusual phenotypes or hosts, comparatively to the typical strains that belong to the salmonicida subspecies. this study focuses on an uncharacterized atypical strain, m18076-11, isolated from lumpfish (cyclopterus lumpus) and not part of the four recognized aeromonas salmonicida subspecies. this isolate presents an unreported phenotype in the a. ... | 2018 | 29315617 |
| the role for the small cryptic plasmids as moldable vectors for genetic innovation in aeromonas salmonicida subsp. salmonicida. | in aeromonas salmonicida subsp. salmonicida, a bacterium that causes fish disease, there are two types of small plasmids (<15 kbp): plasmids without known function, called cryptic plasmids, and plasmids that bear beneficial genes for the bacterium. four among them are frequently detected in strains of a. salmonicida subsp. salmonicida: pasa1, pasa2, pasa3, and pasal1. the latter harbors a gene which codes for an effector of the type three secretion system, while the three others are cryptic. it ... | 2017 | 29326751 |