Publications

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death of methadone users in the district of columbia. 20154790688
[simulation studies on the behavior of various automatic diagnostic procedures]. 20154617105
information processing and automatic diagnosis of circulatory system--introductory remarks from engineer's point of view. 20154610203
[hazards of electrical equipment]. 20154483657
the pensacola story. 20154551267
[comparative study of the incidence of a number of diseases in children, vaccinated with akds vaccines with varying antigenic and quantitative composition of the pertussis component]. 20154785836
two catechol siderophores, acinetobactin and amonabactin, are simultaneously produced by aeromonas salmonicida subsp. salmonicida sharing part of the biosynthetic pathway.the iron uptake mechanisms based on siderophore synthesis used by the fish pathogen aeromonas salmonicida subsp. salmonicida are still not completely understood, and the precise structure of the siderophore(s) is unknown. the analysis of genome sequences revealed that this bacterium possesses two gene clusters putatively involved in the synthesis of siderophores. one cluster is a candidate to encode the synthesis of acinetobactin, the siderophore of the human pathogen acinetobacter baumannii, wh ...201526463084
antimicrobial resistance and plasmid profile of bacterial strains isolated from the urbanized eltsovka-1 river (russia).antimicrobial resistance and plasmid profile of gram-positive and gram-negative bacterial strains isolated from the urbanized eltsovka-1 river (russia) were investigated. sequencing of the 16s rrna of of g+ strains showed 99-100% identity to that of bacillus aerophilus, bacillus altitudinis, bacillus amyloliquefaciens, bacillus anthrancis, bacillus barbaricus, bacillus cereus, bacillus flexus, bacillus indriensis, bacillus stratosphericus, bacillus subtilis subsp. subtilis, bacillus thuringiensi ...201525803299
transcription factor t-bet in atlantic salmon: characterization and gene expression in mucosal tissues during aeromonas salmonicida infection.the t-box transcription factor t-bet is expressed in a number of hematopoietic cell types in mammals and plays an essential role in the lineage determination of th1 t-helper cells and is considered as an essential feature for both innate and adaptive immune responses in higher vertebrates. in the present study, we have identified and characterized the full-length atlantic salmon t-bet cdna (3502 bp). the putative primary structure of the polypeptide deduced from the cdna sequence contained 612 a ...201526217339
screening for antibacterial activity of some turkish plants against fish pathogens: a possible alternative in the treatment of bacterial infections.the antibacterial activity of ethanolic and aqueous crude extracts from 36 plants in turkey, including seven endemic species, against fish pathogens was studied using the disc diffusion assay. the extract that was most active against all microbial strains, except aeromonas salmonicida, was that of dorycnium pentaphyllum. some of the extracts also showed a very broad spectrum of potent antimicrobial activity. the extract of anemone nemorosa showed the highest antimicrobial activity against vibrio ...201526019642
living in an extremely polluted environment: clues from the genome of melanin-producing aeromonas salmonicida subsp. pectinolytica 34melt.aeromonas salmonicida subsp. pectinolytica 34mel(t) can be considered an extremophile due to the characteristics of the heavily polluted river from which it was isolated. while four subspecies of a. salmonicida are known fish pathogens, 34mel(t) belongs to the only subspecies isolated solely from the environment. genome analysis revealed a high metabolic versatility, the capability to cope with diverse stress agents, and the lack of several virulence factors found in pathogenic aeromonas. the mo ...201526025898
comparative evaluation of the antimicrobial activity of different antimicrobial peptides against a range of pathogenic bacteria.the rapid emergence of resistance to classical antibiotics has increased the interest in novel antimicrobial compounds. antimicrobial peptides (amps) represent an attractive alternative to classical antibiotics and a number of different studies have reported antimicrobial activity data of various amps, but there is only limited comparative data available. the mode of action for many amps is largely unknown even though several models have suggested that the lipopolysaccharides (lps) play a crucia ...201526656394
detection and diversity of aeromonads from treated wastewater and fish inhabiting effluent and downstream waters.a two-season investigation of the wastewater treatment plant (wwtp) effluent, of related waters, sludge and fish across a wide area and 11 stations, with emphasis on aeromonas spp. was conducted. aeromonas veronii was the prevailing aeromonad isolated by maldi tof ms in the summer period. a rise of aeromonas hydrophila was observed in summer in raw sewage, treated wastewater and effluent-carrying canal. the ratio of aeromonad species retrieved from fish tissues did not correspond with the water ...201526092555
pro-inflammatory caspase-1 activation during the immune response in cells from rainbow trout oncorhynchus mykiss (walbaum 1792) challenged with pathogen-associated molecular patterns.in response to pathogens, the higher vertebrate innate immune system activates pro-inflammatory caspase-1 which is responsible for the processing and secretion of several important cytokines involved in the host's defence against infection. to date, caspase-1 has been described in few teleost fish, and its activity has been demonstrated through substrate cleavage and inhibition by pharmacological agents. in this study, the detection of the active form of caspase-1 during the immune response in s ...201525477241
[reactivity of the vasomotor system in children with hereditary disposition to hypertension]. 20154655240
[diagnostic problems in neoplastic metastases to the choroid]. 20154690015
predicting optometry grades from the ocat and preoptometry grades. 20154525062
the effect of azide on phototaxis in chlamydomonas reinhardi.phototaxis in chlamydomonas reinhardi was specifically inhibited by azide. the effect of azide was rapid and reversible, and did not depend upon the intensity of actinic light. under conditions of completely inhibited phototaxis, azide had no effect on the number of motile cells in the population or on the rate of motility. the effect was not related to changes in oxygen uptake or cellular atp concentration. apparently, a cellular component or process specifically involved in phototaxis is inact ...20154525466
mbo goes to work in the public sector. 20154492157
[spinal infarction due to nucleus pulposus emboli (author's transl)]. 20154451034
dermatitis caused by the bat mite, chiroptonyssus robustipes (ewing), in california. 20154472534
[surgical pleural biopsy. its diagnostic value]. 20165042121
utilization of general practitioners for drug evaluation. 20164984271
[development of dermatology in the bialystok province during the 25 years of existence of the polish people's republic]. 20164938586
microcirculation of the perioral regions in the macaca rhesus. i. 20164985601
[position of doctor o'meara at saint helena]. 20164905449
letter: particulate contamination--let's solve the problem. 20164810163
antimicrobial activities of bacteria associated with the brown alga padina pavonica.macroalgae belonging to the genus padina are known to produce antibacterial compounds that may inhibit growth of human- and animal pathogens. hitherto, it was unclear whether this antibacterial activity is produced by the macroalga itself or by secondary metabolite producing epiphytic bacteria. here we report antibacterial activities of epiphytic bacteria isolated from padina pavonica (peacocks tail) located on northern coast of tunisia. eighteen isolates were obtained in pure culture and tested ...201627462308
evaluation of different conditions and culture media for the recovery of aeromonas spp. from water and shellfish samples.to perform a comparative study for determining the optimum culture method (direct plating or enrichment) and medium (ampicillin dextrin agar (ada), starch ampicillin agar (saa), bile salts irgasan brilliant green modified (bibg-m)) for recovering aeromonas species from water and shellfish samples.201627333573
the flgt protein is involved in aeromonas hydrophila polar flagella stability and not affects anchorage of lateral flagella.aeromonas hydrophila sodium-driven polar flagellum has a complex stator-motor. consist of two sets of redundant and non-exchangeable proteins (poma/pomb and poma2/pomb2), which are homologs to other sodium-conducting polar flagellum stator motors; and also two essential proteins (motx and moty), that they interact with one of those two redundant pairs of proteins and form the t-ring. in this work, we described an essential protein for polar flagellum stability and rotation which is orthologs to ...201627507965
toll-like receptors in maraena whitefish: evolutionary relationship among salmonid fishes and patterns of response to aeromonas salmonicida.toll-like receptors (tlrs) interact directly with particular pathogenic structures and are thus highly important to innate immunity. the present manuscript characterises a suite of 14 tlrs in maraena whitefish (coregonus maraena), a salmonid species with increasing importance for aquaculture. whitefish tlrs were structurally and evolutionary analysed. the results revealed a close relationship with tlrs from salmonid fish species rainbow trout and atlantic salmon. profiling the baseline expressio ...201627131902
antimicrobial activities of secondary metabolites and phylogenetic study of sponge endosymbiotic bacteria, bacillus sp. at agatti island, lakshadweep archipelago.twenty-one species of sponges were recorded under the class of demospongiae and calcareous sponges of which 19 species were new to agatti reef. a total of 113 sponge endosymbiotic bacterial strains were isolated from twenty-one species of sponges and screened for antimicrobial activity. five bacterial strains of sponge endosymbiotic bacteria (seb) namely seb32, seb33, seb36, seb43 and seb51 showed antimicrobial activity against virulent marine fish pathogens such as vibrio alginolyticus, vibrio ...201628352539
the membership of the omaha dental assistants' society--1970. 20164916379
[hans bulach von rottweil]. 20164906056
varicella pneumonia. treatment with tracheostomy and mechanical endotracheal positive-pressure ventilation. 20164860512
[la fontaine and the apothecaries]. 20164929537
blood, urine, and dietary pantothenic acid levels of pregnant teenagers. 20165021930
comment on the method of determining the trapped volume of plasma after centrifugation, based on the correlation between the trapped volume and the electrical conductivity. 20164964894
[liability declaration for compensation in injuries]. 20165023823
[thermal control of the luminescence emitted by xanthine-oxidase]. 20164995043
[some problems of childhood enuresis. its relation to urinary tract diseases and abnormalities]. 20165088629
vasectomy. 20165067588
[early postoperative rehabilitation after maxillary resection]. 20164951118
[clinical examination of patients with multiple injuries]. 20165078491
blending the best of both worlds--nursing in ghana. 20164845085
aeromonas salmonicida infection levels in pre- and post-stocked cleaner fish assessed by culture and an amended qpcr assay.due to increasing resistance to chemical therapeutants, the use of 'cleaner fish' (primarily wrasse, labridae, species) has become popular in european salmon farming for biocontrol of the salmon louse, lepeophtheirus salmonis (krøyer). while being efficient de-licers, cleaner fish mortality levels in salmon cages are commonly high, and systemic bacterial infections constitute a major problem. atypical furunculosis, caused by aeromonas salmonicida a-layer types v and vi, is among the most common ...201626514414
high concentration of red clay as an alternative for antibiotics in aquaculture.the use of antibiotics in aquaculture raises environmental and food safety concerns because chronic exposure of an aquatic ecosystem to antibiotics can result in the spread of antibiotic resistance, bioaccumulation of antibiotics in the organisms, and transfer of antibiotics to humans. in an attempt to overcome these problems, high-concentration red clay was applied as an alternative antibiotic against the following common fish pathogens: aeromonas salmonicida, vibrio alginolyticus, and streptoc ...201626528535
antigens of aeromonas salmonicida subsp. salmonicida specifically induced in vivo in oncorhynchus mykiss. 201626661507
vapa (a-layer) typing differentiates aeromonas salmonicida subspecies and identifies a number of previously undescribed subtypes.sequence variation in a region of the virulence array protein gene (vapa; a-layer) was assessed in 333 ('typical' and 'atypical') isolates of the fish pathogenic bacterium aeromonas salmonicida. resulting similarity dendrograms revealed extensive heterogeneity, with nearly all isolates belonging to either of 14 distinct clusters or a-layer types. all acknowledged a. salmonicida subspecies (except ssp. pectinolytica, from which no vapa sequence could be obtained) were clearly separated, and notab ...201625846742
development of similar broth microdilution methods to determine the antimicrobial susceptibility of flavobacterium columnare and f. psychrophilum.flavobacterium columnare and f. psychrophilum are major fish pathogens that cause diseases that may require antimicrobial therapy. choice of appropriate treatment is dependent upon determining the antimicrobial susceptibility of isolates. therefore we optimized methods for broth microdilution testing of f. columnare and f. psychrophilum to facilitate standardizing an antimicrobial susceptibility test. we developed adaptations to make reproducible broth inoculums and confirmed the proper incubati ...201626949840
the aeromonas salmonicida lipopolysaccharide core from different subspecies: the unusual subsp. pectinolytica.initial hydridization tests using aeromonas salmonicida typical and atypical strains showed the possibility of different lipopolysaccharide (lps) outer cores among these strains. by chemical structural analysis, lps-core sds-page gel migration, and functional and comparative genomics we demonstrated that typical a. salmonicida (subsp. salmonicida) strains and atypical subsp. masoucida and probably smithia strains showed the same lps outer core. a. salmonicida subsp. achromogenes strains show a s ...201626904002
investigations on the role of the salmon louse, lepeophtheirus salmonis (caligidae), as a vector in the transmission of aeromonas salmonicida subsp. salmonicida.a bacteria-parasite challenge model was used to study the role of sea lice, lepeophtheirus salmonis (copepoda), as a vector of aeromonas salmonicida subsp. salmonicida. three hypotheses were tested: (i) l. salmonis can acquire a. salmonicida subsp. salmonicida via water bath exposure; (ii) l. salmonis can acquire the bacteria via parasitizing infected atlantic salmon, salmo salar; and (iii) l. salmonis can transmit the bacteria to naïve atlantic salmon via parasitism. adult l. salmonis exposed t ...201626851068
challenge models for atypical aeromonas salmonicida and vibrio anguillarum in farmed ballan wrasse (labrus bergylta) and preliminary testing of a trial vaccine against atypical aeromonas salmonicida. 201626849912
increasing genomic diversity and evidence of constrained lifestyle evolution due to insertion sequences in aeromonas salmonicida.aeromonads make up a group of gram-negative bacteria that includes human and fish pathogens. the aeromonas salmonicida species has the peculiarity of including five known subspecies. however, few studies of the genomes of a. salmonicida subspecies have been reported to date.201626753691
metabolic profiling in kidneys of atlantic salmon infected with aeromonas salmonicida based on (1)h nmr.aeromonas salmonicida, an important pathogenic bacterium which induces furunculosis, is globally causing increased risks in atlantic salmon (salmo salar) farming. although the kidney is the main target organ of a. salmonicida, the metabolic profiling of kidney in response to a. salmonicida in vivo remains unknown. here, we used (1)h nuclear magnetic resonance (nmr) to comprehensively analyze the metabolic changes in the kidney of atlantic salmon. through the noesypr1d spectrum combined with mult ...201627577538
diversity of antibiotic-resistance genes in canadian isolates of aeromonas salmonicida subsp. salmonicida: dominance of psn254b and discovery of pasa8.the bacterium aeromonas salmonicida subsp. salmonicida is a common pathogen in fish farms worldwide. since the antibiotic resistance of this bacterial species is on the increase, it is important to have a broader view on this issue. in the present study, we tested the presence of known plasmids conferring multi-drug resistance as well as antibiotic resistance genes by a pcr approach in 100 canadian a. salmonicida subsp. salmonicida isolates. our study highlighted the dominance of the conjugative ...201627752114
cross talk between growth and immunity: coupling of the igf axis to conserved cytokine pathways in rainbow trout.although disease and infection is associated with attenuated growth, the molecular pathways involved are poorly characterized. we postulated that the igf axis, a central governor of vertebrate growth, is repressed during infection to promote resource reallocation towards immunity. this hypothesis was tested in rainbow trout (oncorhynchus mykiss) challenged by aeromonas salmonicida (as), a gram-negative bacterial pathogen, or viral hemorrhagic septicemia virus (vhsv) at hatch, first feeding, and ...201627035654
antibiotic resistance due to an unusual cole1-type replicon plasmid in aeromonas salmonicida.aeromonas salmonicida subsp. salmonicida is a fish pathogen known to have a rich plasmidome. in the present study, we discovered an isolate of this bacterium bearing an additional unidentified small plasmid. after having sequenced the dna of that isolate by next-generation sequencing, it appeared that the new small plasmid is a cole1-type replicon plasmid, named here pasa7. this plasmid bears a functional chloramphenicol-acetyltransferase-encoding gene (cat-pasa7) previously unknown in a. salmon ...201627028891
facile recoding of selenocysteine in nature.selenocysteine (sec or u) is encoded by uga, a stop codon reassigned by a sec-specific elongation factor and a distinctive rna structure. to discover possible code variations in extant organisms we analyzed 6.4 trillion base pairs of metagenomic sequences and 24 903 microbial genomes for trna(sec) species. as expected, uga is the predominant sec codon in use. we also found trna(sec) species that recognize the stop codons uag and uaa, and ten sense codons. selenoprotein synthesis programmed by ua ...201626991476
development of a sybr green i real-time pcr assay for specific identification of the fish pathogen aeromonas salmonicida subspecies salmonicida.a sybr green i real-time polymerase chain reaction protocol for specific detection of the fish pathogen aeromonas salmonicida subsp. salmonicida was developed and validated for rapid diagnosis of typical furunculosis. the sequence of the aopo gene of a. salmonicida subsp. salmonicida, which encodes for a serine/threonine protein kinase linked to virulence, was chosen for primer design. the selected primers amplified a 119-bp internal fragment of the aopo gene. the specificity test proved that 10 ...201627838837
aeromonas salmonicida: updates on an old acquaintance.aeromonas salmonicida is the oldest known infectious agent to be linked to fish disease and constitutes a major bacterial pathogen of fish, in particular of salmonids. this bacterium can be found almost worldwide in both marine and freshwater environments and has been divided into several sub-species. in this review, we present the most recent developments concerning our understanding of this pathogen, including how the characterization of new isolates from non-salmonid hosts suggests a more nua ...201627304870
expression and antimicrobial activity of c-type lysozyme in taimen (hucho taimen, pallas).lysozymes are important defense proteins of the innate immune system and possess high antibacterial activities. in the present study, a full-length c-type lysozyme cdna (htlysc) was cloned and characterized from taimen (hucho taimen, pallas). the cdna contains an open reading frame (orf) of 432 bp encoding 143 amino acid (aa), with 97% identity to lysc of rainbow trout (oncorhynchus mykiss). the amino acid sequence possessed a lyz1 domain (16-140 aa) which contained two conserved residues (glu 5 ...201627267655
preliminary study on the in vitro and in vivo effects of asparagopsis taxiformis bioactive phycoderivates on teleosts.several compounds from marine organisms have been studied for their potential use in aquaculture. among the red algae, asparagopsis taxiformis is considered one of the most promising species for the production of bioactive metabolites with numerous proposed applications. here, the in vitro antibacterial activity, the easy handling and the absence of adverse effects on marine fish species are reported. depending on the seasonal period of sampling, ethanol extracts of a. taxiformis exhibited signi ...201627826246
the mosaic architecture of aeromonas salmonicida subsp. salmonicida pasa4 plasmid and its consequences on antibiotic resistance.aeromonas salmonicida subsp. salmonicida, the causative agent of furunculosis in salmonids, is an issue especially because many isolates of this bacterium display antibiotic resistances, which limit treatments against the disease. recent results suggested the possible existence of alternative forms of pasa4, a large plasmid found in a. salmonicida subsp. salmonicida and bearing multiple antibiotic resistance genes. the present study reveals the existence of two newly detected pasa4 variants, pas ...201627812409
pathogen-dependent role of turbot (scophthalmus maximus) interferon-gamma.interferon-gamma has been typically described as a pro-inflammatory cytokine playing an important role in the resolution of both viral and bacterial diseases. nevertheless, some anti-inflammatory functions are also attributed to this molecule. in this work we have characterized for the first time the turbot (scophthalmus maximus) interferon-gamma gene (ifng) and its expression pattern under basal conditions, after type i ifns administration, and viral and bacterial infection. the intramuscular i ...201627742586
a comparison of the response of diploid and triploid atlantic salmon (salmo salar) siblings to a commercial furunculosis vaccine and subsequent experimental infection with aeromonas salmonicida.sterile triploid fish represent a solution to the problems associated with sexual maturation and escapees in aquaculture. however, as disease outbreaks continue to cause significant economic losses to the industry, it is essential that the response of triploids to disease and disease treatments be characterised. the aim of this study was to compare the response of triploid atlantic salmon to a commercial furunculosis vaccine with that of diploid fish, and to assess the vaccine efficacy in the tw ...201627569980
structural evidence of intramolecular propeptide inhibition of the aspzincin metalloendopeptidase asap1.the gram-negative bacterium aeromonas salmonicida is a fish pathogen for various fish species worldwide. aeromonas salmonicida subsp. achromogenes produces the extracellular, toxic zinc endopeptidase asap1. crystal structure analyses at 2.0 å resolution of two proteolytically inactive asap1 variants show the polypeptide folding of the protease domain and the propeptide domain. these first crystal structure analyses of a precursor of a deuterolysin-like aspzincin protease provide insights into pr ...201627528449
aeromonas salmonicida subsp. salmonicida strains isolated from chinese freshwater fish contain a novel genomic island and possible regional-specific mobile genetic elements profiles.two strains of aeromonas salmonicida, yk and bg, were isolated from largemouth bronze gudgeon and northern whitefish in china, and identified as a. salmonicida subsp. salmonicida based on phylogenetic analysis of vapa and 16s rrna gene sequences. yk and bg originated from freshwater fish, one of which belonged to the cyprinid family, and the strains showed a difference in virulence. subsequently, we performed whole genome sequencing of the strains, and comparison of their genomic sequences to th ...201627493011
alterations of the gut microbiome of largemouth bronze gudgeon (coreius guichenoti) suffering from furunculosis.high-throughput sequencing was applied to compare the intestinal microbiota in largemouth bronze gudgeon either healthy or affected by furunculosis. proteobacteria, actinobacteria, tenericutes, firmicutes and bacteroidetes were detected as the predominant bacterial phyla in the gut of both diseased and healthy fish. the abundance of proteobacteria differed significantly between the two groups of fish, mainly due to the overwhelming prevalence of aeromonas in the diseased fish (81% ± 17%), while ...201627465687
immunohistochemical study of inducible nitric oxide synthase and tumour necrosis factor alpha response in turbot (scophthalmus maximus) experimentally infected with aeromonas salmonicida subsp. salmonicida.aeromonas salmonicida subsp. salmonicida represents one of the major threats in aquaculture, especially in salmonid fish and turbot farming. in order to fight bacterial infections, fish have an immune system composed by innate and specific cellular and humoral elements analogous to those present in mammals. however, innate immunity plays a primordial role against bacterial infections in teleost fish. among these non-specific mechanisms, the production of nitric oxide (no) by inducible nitric oxi ...201627431586
the effects of increasing dietary levels of soy protein concentrate (spc) on the immune responses and disease resistance (furunculosis) of vaccinated and non-vaccinated atlantic salmon (salmo salar l.) parr.juvenile salmon, with an initial weight of 9 g, were fed three experimental diets, formulated to replace 35 (spc35), 58 (spc58) and 80 (spc80) of high quality fishmeal (fm) with soy protein concentrate (spc) in quadruplicate tanks. higher dietary spc inclusion was combined with increased supplementation of methionine, lysine, threonine and phosphorus. the experiment was carried out for 177 days. on day 92 salmon in each tank were bulk weighed. post weighing eighty salmon from each tank were redi ...201627742588
[studies of prevalence rate of furunculosis caused by infection by aeromonas salmonicida in salmonids of the southern part of sakhalin island].ichthyological studies of spawners of salmonids in the south of sakhalin island were studied. cases of furunculosis disease were revealed. the agent of the disease aeromonas salmonicida was isolated. its morphological, physiological-biochemical, and antagonistic properties were studied, and the virulence of the isolated strains was determined. for supporting the species status of the studied strains of a. salmonicida, a molecular-genetic analysis was performed.201623136736
external rotation palsy of shoulder. 20165024524
[functional and organic tricuspid insufficiency. (apropos of 60 cases studied by catheterization and intracavitary phonocardiography)]. 20164970129
the role of interoreceptors in the mechanism of action of the hormones. 20164959480
subacute sclerosing encephalitis. 20164862504
mineralization of the bone in an insured population: correlation with reported fractures and other measures of osteoporosis. 20164776018
are you receiving me? 20165014404
psychiatric in-patient unit for young adolescents. 20164884563
rejection of the generic name dendrocystis rees, 1962, and its replacement by arborocystis nov.nom. protozoa: gregarinomorpha. 20164960245
[renal transplantation: problems of organ donors]. 20164948828
[application of isotopes to heart-pulmonary circulation dynamics]. 20164871482
we broke the communication barrier. 20165076704
hospital indicators. 20164820306
degradation products and the role of coagulation in "persistent" glomerulonephritis. 20164931741
effects of anticoagulants and containers (glass and plastic) on the blood count. 20164999452
the case of the greasy spoon. 20165031569
are all those x-rays and tests really necessary? 20165010913
[diagnosis and first aid in drug intoxications in small children]. 20165041338
[a little tip on the problem of temporary dentures]. 20164944957
the pragmatic utilization of the p-k phenomenon. 20165039530
in vitro assessment of the antimicrobial activity of silver and zinc oxide nanoparticles against fish pathogens.antibiotic resistance is a global issue that threatens public health. the excessive use of antibiotics contributes to this problem as the genes of antibiotic resistance can be transferred between the bacteria in humans, animals and aquatic organisms. metallic nanoparticles could serve as future substitutes for some conventional antibiotics because of their antimicrobial activity. the aim of this study was to evaluate the antimicrobial effects of silver and zinc oxide nanoparticles against major ...201728732514
analysis of the genome and mobilome of a dissimilatory arsenate reducing aeromonas sp. o23a reveals multiple mechanisms for heavy metal resistance and metabolism.aeromonas spp. are among the most ubiquitous microorganisms, as they have been isolated from different environmental niches including waters, soil, as well as wounds and digestive tracts of poikilothermic animals and humans. although much attention has been paid to the pathogenicity of aeromonads, the role of these bacteria in environmentally important processes, such as transformation of heavy metals, remains to be discovered. therefore, the aim of this study was a detailed genomic characteriza ...201728611742
characterization of the cultivable gut microflora in wild-caught 
mediterranean fish species.microflora of the gastrointestinal tract plays important roles in food digestion, nutrient absorption and in host defense against ingested pathogens. several studies have focused on the microflora of farmed fishes, but the gut flora of wild fishes remains poorly characterized. the aim of this work was to provide an overview of the bacteria colonizing the gut of wild-caught fishes and to determine whether some bacterial species can be pathogenic.201728553195
chromosome-mediated mcr-3 variants in aeromonas veronii from chicken meat.two adjacent colistin resistance gene variants, termed mcr-3.2 and mcr-3-like, were identified in the chromosome of an aeromonas veronii isolated from retail chicken meat. the variants showed 95.20% and 84.19% nucleotide sequence identity, respectively, to mcr-3 from porcine escherichia coli functional cloning indicated that only mcr-3.2 conferred polymyxin resistance in both e. coli and aeromonas salmonicida the mcr-3.2-mcr-3-like segment was also observed in other aeromonas species, including ...201728848017
characterization and diversity of phages infecting aeromonas salmonicida subsp. salmonicida.phages infecting aeromonas salmonicida subsp. salmonicida, the causative agent of the fish disease furunculosis, have been isolated for decades but very few of them have been characterized. here, the host range of 12 virulent phages, including three isolated in the present study, was evaluated against a panel of 65 a. salmonicida isolates, including representatives of the psychrophilic subspecies salmonicida, smithia, masoucida, and the mesophilic subspecies pectinolytica. this bacterial set als ...201728765570
a historical review of the key bacterial and viral pathogens of scottish wild fish.thousands of scottish wild fish were screened for pathogens by marine scotland science. a systematic review of published and unpublished data on six key pathogens (renibacterium salmoninarum, aeromonas salmonicida, ipnv, isav, sav and vhsv) found in scottish wild and farmed fish was undertaken. despite many reported cases in farmed fish, there was a limited number of positive samples from scottish wild fish, however, there was evidence for interactions between wild and farmed fish. a slightly el ...201728718925
plasmid composition in aeromonas salmonicida subsp. salmonicida 01-b526 unravels unsuspected type three secretion system loss patterns.aeromonas salmonicida subsp. salmonicida is a ubiquitous psychrophilic waterborne bacterium and a fish pathogen. the numerous mobile elements, especially insertion sequences (is), in its genome promote rearrangements that impact its phenotype. one of the main virulence factors of this bacterium, its type three secretion system (ttss), is affected by these rearrangements. in aeromonas salmonicida subsp. salmonicida most of the ttss genes are encoded in a single locus on a large plasmid called pas ...201728701230
novel plasmid-mediated colistin resistance gene mcr-3 in escherichia coli.the mobile colistin resistance gene mcr-1 has attracted global attention, as it heralds the breach of polymyxins, one of the last-resort antibiotics for the treatment of severe clinical infections caused by multidrug-resistant gram-negative bacteria. to date, six slightly different variants of mcr-1, and a second mobile colistin resistance gene, mcr-2, have been reported or annotated in the genbank database. here, we characterized a third mobile colistin resistance gene, mcr-3 the gene coexisted ...201728655818
the protective effect of humic-rich substances on atypical aeromonas salmonicida subsp. salmonicida infection in common carp (cyprinus carpio l.).when challenged with atypical aeromonas salmonicida subsp. salmonicida, exposure of the common carp (cyprinus carpio l.) to different humic-rich compounds resulted in a significant reduction in infection rates. specifically, in fish exposed to (i) humic-rich water and sludge from a recirculating system, (ii) a synthetic humic acid, and (iii) a leonardite-derived humic-rich extract, infection rates were reduced to 14.9%, 17.0% and 18.8%, respectively, as compared to a 46.8% infection rate in the ...201728493490
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