Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| a computer assisted instructional course in diagnosis and treatment of the rheumatic diseases. | 2014 | 4139957 | |
| transcriptome profiling reveals insight into distinct immune responses to aeromonas salmonicida in gill of two rainbow trout strains. | the fish gills represent a crucial organ for the communication with the aquatic environment. transcriptional changes in gills of two hatchery rainbow trout strains in response to injection with the potent pathogen aeromonas salmonicida were detected by global gene expression profiling using a 4×44k oligonucleotide microarray. emphasis was placed on "day 3 postinfection" representing a decisive time point for the resolution of inflammation. the comparison of features and pathways differentially r ... | 2014 | 24122123 |
| aeromonas salmonicida subsp. salmonicida in the light of its type-three secretion system. | aeromonas salmonicida subsp. salmonicida is an important pathogen in salmonid aquaculture and is responsible for the typical furunculosis. the type-three secretion system (t3ss) is a major virulence system. in this work, we review structure and function of this highly sophisticated nanosyringe in a. salmonicida. based on the literature as well as personal experimental observations, we document the genetic (re)organization, expression regulation, anatomy, putative functional origin and roles in t ... | 2014 | 24119189 |
| virulence, genomic features, and plasticity of aeromonas salmonicida subsp. salmonicida, the causative agent of fish furunculosis. | the bacterium aeromonas salmonicida subsp. salmonicida is the causative agent of furunculosis, a systemic disease of fish in the salmonid family. furunculosis is a ubiquitous disease that affects aquaculture operations worldwide and is characterized by high mortality and morbidity. a better understanding of the bacterium is required to find a cure. thereby, this review centers on a. salmonicida subsp. salmonicida, its major virulence factors, and its genome. the classification and characteristic ... | 2014 | 23890675 |
| the effects of benzo[a]pyrene on leucocyte distribution and antibody response in rainbow trout (oncorhynchus mykiss). | polycyclic aromatic hydrocarbons (pahs) are a group of compounds with immunotoxic and carcinogenic potential that may pose a threat to fish populations. this study aims to utilize a newly developed fish immunotoxicology model to determine the immune tissue/cell population level effects of pahs on rainbow trout, using benzo[a]pyrene (bap) as a representative immunotoxic pah. intraperitoneal injection of 25 or 100mg/kg bap resulted in sustained exposure as indicated by biliary fluorescence at bap ... | 2014 | 24398112 |
| optimization of a plasmid electroporation protocol for aeromonas salmonicida subsp. salmonicida. | aeromonas salmonicida subsp. salmonicida is a major fish pathogen. molecular tools are required to study the virulence and genomic stability of this bacterium. an efficient electroporation-mediated transformation protocol for a. salmonicida subsp. salmonicida would make genetic studies faster and easier. in the present study, we designed the 4.1-kb psdd1 plasmid as a tool for optimizing an electroporation protocol for a. salmonicida subsp. salmonicida. we systematically tested the electroporatio ... | 2014 | 24389038 |
| enhanced aeromonas salmonicida bacterin uptake and side effects caused by low frequency sonophoresis in rainbow trout (oncorhynchus mykiss). | low frequency sonophoresis (lfs) has been recognized as one of the most advanced technologies in transdermal delivery of substances, due to the modification of the stratum corneum lipid bilayer, in focal skin applications in mammals. based on these findings, lfs has been suggested as a potential technology to be used for enhancement in immersion fish vaccination. in contrast to mammals where lfs is applied to discrete regions of the skin, in fish the whole individual needs to be exposed for prac ... | 2014 | 24378683 |
| comparision of a serological potency assay for furunculosis vaccines (aeromonas salmonicida subsp. salmonicida) to intraperitoneal challenge in atlantic salmon (salmo salar l.). | batch potency testing of salmonid vaccines is mainly performed by in vivo challenge, which requires a lot of animals and causes severe pain. due to the animal welfare concerns associated with in vivo immunization challenge tests, methods which could refine, reduce or replace (3rs) these tests are needed. the aim of this study was to assess the use of serological assay (immunization & antibody estimation with an enzyme-linked immunosorbent assay (elisa) for batch potency testing of oil adjuvanted ... | 2014 | 24359994 |
| characterization of the interleukin 1 receptor-associated kinase 4 (irak4)-encoding gene in salmonid fish: the functional copy is rearranged in oncorhynchus mykiss and that factor can impair tlr signaling in mammalian cells. | the interleukin 1 receptor-associated kinase 4 (irak4) is an essential factor for tlr-mediated activation of the host's immune functions subsequent to pathogen contact. we have characterized the respective cdna and gene sequences from three salmonid species, salmon, rainbow trout and maraena whitefish. the gene from salmon is structured into eleven exons, as is the mammalian homologue, while exons have been fused in the genes from the two other salmonid species. rainbow trout expresses also a ps ... | 2014 | 24239597 |
| altered gene expression patterns of innate and adaptive immunity pathways in transgenic rainbow trout harboring cecropin p1 transgene. | we have recently developed several homozygous families of transgenic rainbow trout harbouring cecropin p1 transgene. these fish exhibit resistance characteristic to infection by aeromonas salmonicida and infectious hematopoietic necrosis virus (ihnv). in our earlier studies we have reported that treatment of a rainbow trout macrophage cell line (rts11) with a linear cationic α-helical antimicrobial peptide (e.g., cecropin b) resulted in elevated levels of expression of two pro-inflammatory relev ... | 2014 | 25306446 |
| aeromonas salmonicida binds differentially to mucins isolated from skin and intestinal regions of atlantic salmon in an n-acetylneuraminic acid-dependent manner. | aeromonas salmonicida subsp. salmonicida infection, also known as furunculosis disease, is associated with high morbidity and mortality in salmonid aquaculture. the first line of defense the pathogen encounters is the mucus layer, which is predominantly comprised of secreted mucins. here we isolated and characterized mucins from the skin and intestinal tract of healthy atlantic salmon and studied how a. salmonicida bound to them. the mucins from the skin, pyloric ceca, and proximal and distal in ... | 2014 | 25287918 |
| growth ability of gram negative bacteria in free-living amoebae. | when bacteria and free-living amoebae (flas) live both in natural waters and man-made aquatic systems, they constantly interact with each other. some bacteria can survive and grow within flas. therefore, it has recently been thought that flas play an important role in spreading pathogenic bacteria in aquatic systems. in this study we investigated the intracellular growing ability of 7 different gram-negative bacteria (pseudomonas fluorescens, pseudomonas putida, pasteurella pneumotropica, aeromo ... | 2014 | 24955522 |
| lipopolysaccharides isolated from aeromonas salmonicida and vibrio anguillarum show quantitative but not qualitative differences in inflammatory outcome in sparus aurata (gilthead seabream). | in fish, the defence system recognises pathogenic microorganisms via pathogen recognition receptors (prrs) that sense particular structures of the pathogens; the so-called pathogen associated molecular patterns (pamps) such as bacterial lipopolysaccharides (lpss). the result of the pamp-prr interactions leads to complex and orchestrated immune responses. in this study, sparus aurata (gilthead seabream) were intraperitoneally injected with purified lipopolysaccharide (lps) from aeromonas salmonic ... | 2014 | 24954838 |
| aeromonas phages encode trnas for their overused codons. | the gc-rich bacterial species, aeromonas salmonicida, is parasitised by both gc-rich phages (aeromonas phages - phias7 and vb_asam-56) and gc-poor phages (aeromonas phages - 25, 31, 44rr2.8t, 65, aes508, phias4 and phias5). both the gc-rich aeromonas phage phias7 and aeromonas phage vb_asam-56 have nearly identical codon usage bias as their host. while all the remaining seven gc-poor aeromonas phages differ dramatically in codon usage from their gc-rich host. here, we investigated whether trna e ... | 2014 | 24878728 |
| protein expression and transcription profiles of three strains of aeromonas salmonicida ssp. salmonicida under normal and iron-limited culture conditions. | aeromonas salmonicida is an important fish pathogen that produces a wide and varied array of virulence factors. here we used iron deprivation by addition of the chelator 2'2-dipyridyl to induce the expression of several such virulence factors in three isolates of aeromonas salmonicida (one avirulent and two virulent). by using sds-page followed by mass spectrometry, we identified proteins that appeared differentially expressed under these conditions. the differential transcription of the identif ... | 2014 | 24872729 |
| the warm temperature acclimation protein (wap65) has an important role in the inflammatory response of turbot (scophthalmus maximus). | wap65 is a molecule similar to the mammalian hemopexin that is a serum glycoprotein produced mainly by the liver with high affinity to heme. its primary role is participating in iron metabolism scavenging heme that is released into the plasma and transporting it to the liver. it has been reported an important role of hemopexin in the inflammation as an acute-phase protein and its production is up-regulated by pro-inflammatory cytokines. there are also some evidences suggesting this immune-induct ... | 2014 | 24794581 |
| the proximal promoter of a novel interleukin-8-encoding gene in rainbow trout (oncorhynchus mykiss) is strongly induced by cebpa, but not nf-κb p65. | interleukin-8 (il8) is an immediate-early chemokine that has been well characterized in several fish species. ten il8 gene variants have already been described in rainbow trout, but none of their promoters has structurally been defined or functionally characterized in teleost fish. to uncover key factors regulating il8 expression, we intended to functionally characterize an il8 promoter from rainbow trout. incidentally, we isolated a novel il8 gene variant (il8-g). it is structurally highly simi ... | 2014 | 24721762 |
| isolation of aeromonas salmonicida from human blood sample: a case report. | aeromonas salmonicida belonging to the genus aeromonas, is a common pathogen that causes furunculosis and septicaemia in variety of fishes. it infects cold blooded vertebrates living at low temperatures mainly salmonid fish hence named salmonicida. untill recently aeromanas salmonicida is considered to be a fish pathogen. a. salmonicida is considered to be non-pathogenic for humans as it cannot grow at 37ºc. "however, in our laboratory culture plates and broths were incubated twice at 37ºc and e ... | 2014 | 24701507 |
| the first characterization of two type i interferons in turbot (scophthalmus maximus) reveals their differential role, expression pattern and gene induction. | type i interferons (ifns) are considered the main cytokines directing the antiviral immune response in vertebrates. these molecules are able to induce the transcription of interferon-stimulated genes (isgs) which, using different blocking mechanisms, reduce the viral proliferation in the host. in addition, a contradictory role of these ifns in the protection against bacterial challenges using murine models has been observed, increasing the survival or having a detrimental effect depending on the ... | 2014 | 24680948 |
| histamine-producing bacteria in blue scad (decapterus maruadsi) and their abilities to produce histamine and other biogenic amines. | using decarboxylation medium and 16s rdna sequence analysis, histamine-producing bacteria (hpb) in blue scad (decapterus maruadsi) were isolated and identified, and the histamine-producing abilities of the isolated hpb were determined. nine mesophilic strains (h1-h9) isolated from the muscle of blue scad were identified as the genera of hpb, including arthrobacter bergeri (h1), pseudomonas sp. (h2, h5 and h6), psychrobacter sp. (h3), shewanella baltica (h4 and h7), and aeromonas salmonicida (h8 ... | 2014 | 24668182 |
| modulation of cellular innate immunity by lepeophtheirus salmonis secretory products. | lepeophtheirus salmonis produces pharmacologically active substances that have been shown to modify genetic expression of inflammatory mediators in shk-1 cells and head kidney macrophages of salmon. differences in genetic expression among genera of oncorhynchus and salmo reflect different susceptibilities to l. salmonis. this study was conducted to determine if the presence of l. salmonis secretory products (seps)(1) alters the cellular innate immune response (specifically macrophage function) a ... | 2014 | 24657318 |
| in vivo study of spoilage bacteria on polyphenoloxidase activity and melanosis of modified atmosphere packaged pacific white shrimp. | this study investigated the effect of the three spoilage bacteria (carnobacterium maltaromaticum, shewanella putrefaciens and aeromonas salmonicida) on the development of melanosis by inoculating the bacteria on modified atmosphere packaged shrimp. the three bacteria, which inoculated at about 5 log cfu/g, proliferated to a maximum level of 7.49, 6.86 and 6.89 log cfu/g, respectively at the end of storage. in regards to the effect of bacteria on melanosis, it was found that c. maltaromaticum did ... | 2014 | 24594164 |
| the impact of temperature stress and pesticide exposure on mortality and disease susceptibility of endangered pacific salmon. | anthropogenic stressors, including chemical contamination and temperature stress, may contribute to increased disease susceptibility in aquatic animals. specifically, the organophosphate pesticide malathion has been detected in surface waters inhabited by threatened and endangered salmon. in the presence of increasing water temperatures, malathion may increase susceptibility to disease and ultimately threaten salmon survival. this work examines the effect of acute and sublethal exposures to mala ... | 2014 | 24559935 |
| evaluation of the spoilage potential of bacteria isolated from spoiled cooked whole tropical shrimp (penaeus vannamei) stored under modified atmosphere packaging. | the spoilage potential of isolates belonging to five bacterial groups/species (shewanella baltica, carnobacterium maltaromaticum, aeromonas salmonicida, vibrio sp., "other gamma-proteobacteria" [containing one strain of pseudoalteromonas sp. and one strain of psychrobacter sp.]) isolated from spoiled cooked and whole tropical shrimp stored under modified atmosphere packaging (map) was evaluated by inoculation into ionized cooked and peeled tropical shrimp followed by storage for 32 days at 8 °c. ... | 2014 | 24549192 |
| infection of sea lamprey with an unusual strain of aeromonas salmonicida. | the invasion of the laurentian great lakes by the fish-parasitic sea lamprey has led to catastrophic consequences, including the potential introduction of fish pathogens. aeromonas salmonicida is a bacterial fish pathogen that causes devastating losses worldwide. currently, there are five accepted subspecies of aeromonas salmonicida: a. salmonicida subsp. salmonicida, masoucida, smithia, achromogenes, and pectinolytica. we discuss the discovery of an isolate of a. salmonicida that is pathogenic ... | 2014 | 24484501 |
| identification and functional characterization of the goldfish (carassius auratus l.) high mobility group box 1 (hmgb1) chromatin-binding protein. | we report on the identification and functional characterization of hmgb1 of the goldfish. quantitative analysis indicated the highest expression of goldfish hmgb1 in the brain, with lower mrna levels in spleen, intestine, kidney, gill and heart. hmgb1 was also differentially expressed in goldfish immune cell populations with highest mrna levels present in splenocytes and neutrophils. we generated and functionally characterized the recombinant hmgb1 (rghmgb1). the rghmgb1 primed the respiratory b ... | 2014 | 24406304 |
| construction of aeromonas salmonicida subsp. achromogenes asap1-toxoid strains and study of their ability to induce immunity in arctic char, salvelinus alpinus l. | the metalloendopeptidase asap1 is one of the major extracellular virulence factors of a. salmonicida subsp. achromogenes, expressed as a 37-kda pre-pro-peptide and processed to a 19-kda active peptide. the aim of this study was to construct mutant strains secreting an asap1-toxoid instead of asap1-wt, to study virulence of these strains and to test the potency of the asap1-toxoid bacterin and the recombinant asap1-toxoids to induce protective immunity in arctic char. two a. salmonicida mutants w ... | 2014 | 25271952 |
| detection of variants of the pras3, pab5s9, and psn254 plasmids in aeromonas salmonicida subsp. salmonicida: multidrug resistance, interspecies exchanges, and plasmid reshaping. | the ubiquitous water-borne gram-negative bacterium aeromonas salmonicida subsp. salmonicida is the causative agent of furunculosis, a worldwide disease in fish farms. plasmids carrying antibiotic resistance genes have already been described for this bacterium. the aim of the present study was to identify and characterize additional multidrug resistance plasmids in a. salmonicida subsp. salmonicida. we sequenced the plasmids present in two multiple antibiotic-resistant isolates using high-through ... | 2014 | 25267667 |
| [a case of methemoglobinemia m. preliminary note]. | 2014 | 4252102 | |
| malaria in africa. changes in continuity. international colloquium. 14-15 december 1984, antwerpen, belgium. | 2014 | 4073975 | |
| double-peaked contraction waves--a variant of normal. | 2014 | 4007432 | |
| british medical association attacks cigarette smoking. | 2014 | 3978493 | |
| the occurrence of nuclear masses in the pulmonary capillary bed in infants. | 2014 | 4790380 | |
| the antibiotic ya-56 complex: isolation, purification and physicochemical properties of the main components. | 2014 | 4131161 | |
| synovial plasma cells in rheumatoid arthritis. electron microscope and immunofluorescence studies. | 2014 | 4097692 | |
| [sperm motility, its regulation and methods of its recording]. | 2014 | 4040438 | |
| [3-year experience in the treatment with several arrhythmias with ritmos (ajmalin)]. | 2014 | 4184833 | |
| varicose veins: a comparison of surgery and injection-compression sclerotherapy. | 2014 | 4117605 | |
| [endoscopic diagnosis of biliary tract diseases]. | 2014 | 4269963 | |
| [treatment of some otorhinolaryngologic diseases with sea buckthorn oil]. | 2014 | 4272753 | |
| [treatment of some otorhinolaryngologic diseases with sea buckthorn oil]. | 2014 | 4272753 | |
| anti-rh globulin in massachusetts. | 2014 | 4178642 | |
| histochemical method. its evolution and applications. | 2014 | 4105311 | |
| [complication of ureterorenoscopy]. | we describe failures and complications of ureterorenoscopy based on our own experience and the literature. there is usually no consequence when perforating the ureter--which happens in 10 per cent of the cases--if a splint or a percutaneous nephrostomy is applied. ureteral rupture makes an open intervention necessary, but happens rarely. since complications of ureterorenoscopy are rare and the rate of success very high, open operations on the ureter should become less frequent. | 2014 | 4035829 |
| dentistry: a profession at the crossroad. | 2014 | 4232325 | |
| plasma testosterone in idiopathic hirsutism, and the changes produced by adrenal and ovarian stimulation and suppression. | 2014 | 4293667 | |
| [medico-legal considerations apropos of post-traumatic intraperitoneal rupture of a hydatid cyst of the liver]. | 2014 | 4249282 | |
| plasma testosterone in idiopathic hirsutism, and the changes produced by adrenal and ovarian stimulation and suppression. | 2014 | 4293667 | |
| perinatal tips. | 2015 | 4768215 | |
| dental specialties--past, present and future. | 2015 | 4444815 | |
| [impressions of a study trip to japan]. | 2015 | 4495220 | |
| [anatomical contributions of schaffhausen medical school of the 17th century]. | 2015 | 4569048 | |
| [dental caries and their complications]. | 2015 | 4481668 | |
| drug therapy: immunosuppressive agents (second of two parts). | 2015 | 4594161 | |
| the history of biomedical education in the netherlands. | 2015 | 4561822 | |
| what shall dentists do to save the form of man's dentition? | 2015 | 4522580 | |
| the pensacola story. | 2015 | 4551267 | |
| cortical dimensions of second metacarpal in four ethnic groups of south african children. | 2015 | 4693999 | |
| spatial configuration of single-stranded polynucleotides. calculations of average dimensions and nmr coupling constants. | 2015 | 4433699 | |
| [distribution of medications]. | 2015 | 4493468 | |
| cell-surface interactions: differential inhibition by proflavine of embryonic cell aggregation and production of specific cell-aggregating factor. | cell recognition and morphogenetic aggregation of embryonic cells into tissues are mediated by specific macromolecules in the cell surface (cell-aggregating factors). a factor specific for embryonic neural retina cells was demonstrated; its synthesis is required for histotypic reaggregation of retina cell suspensions. we show that proflavine (3,6-diaminoacridine) preferentially and reversibly suppresses production of the cell-aggregating factor and thereby inhibits normal cell reaggregation. if ... | 2015 | 4522293 |
| cadmium uptake and time dependent alterations in tissue levels in the white catfish ictalurus catus (pisces:ictaluridae). | 2015 | 4433805 | |
| [long term results of transtemporal neurectomy of the vestibular nerve in menière's disease (author's transl)]. | 2015 | 4420512 | |
| contraceptive use--efficacy study utilizing medroxyprogesterone acetate administered as an intramuscular injection once every 90 days. | 2015 | 4698589 | |
| on detecting words embedded in a stream of auditory letters. | 2015 | 4656821 | |
| recent developments in antimycotic chemotherapy. | 2015 | 4601865 | |
| [case of multiple molluscum contagiosum of palpebral and facial skin]. | 2015 | 4690011 | |
| [2 cases of rieger's syndrome]. | 2015 | 4690013 | |
| software printout. | 2015 | 4542580 | |
| functional characterization of receptor-interacting serine/threonine kinase 2 (rip2) of the goldfish (carassius auratus l.). | we report on the functional characterization of rip2 of the goldfish. quantitative expression analysis of goldfish rip2 revealed the greatest mrna levels in the spleen, monocytes and splenocytes. we generated a recombinant form of the molecule (rgrip2) and determined that anti-human rip2 polyclonal antibody specifically recognized recombinant goldfish rip2 (rgrip2). goldfish rip2 activity was inhibited by the p38 mapk pathway inhibitor sb203580. treatment of goldfish macrophages with lps, pgn, m ... | 2015 | 25242011 |
| adverse and long-term protective effects following oil-adjuvanted vaccination against aeromonas salmonicida in rainbow trout. | prophylactic measures against aeromonas salmonicida subsp. salmonicida, the causative agent of furunculosis, have been an active field of research for decades, with studies mainly focused on atlantic salmon (salmo salar). in the present study we have examined the protective and adverse effects of mineral oil-adjuvanted injection vaccines on rainbow trout (oncorhynchus mykiss). a commercial vaccine and an experimental auto vaccine, as well as their respective adjuvant formulations alone were used ... | 2015 | 25281580 |
| molecular and functional characterization of goldfish (carassius auratus l.) serum amyloid a. | quantitative expression analysis of goldfish saa revealed the highest mrna levels in the kidney, spleen and intestine with lower mrna levels in muscle and liver. goldfish saa was differentially expressed in goldfish immune cells with highest mrna levels observed in neutrophils. to functionally assess goldfish saa, recombinant protein (rgsaa) was generated by prokaryotic expression and functionally characterized. monocytes and macrophages treated with rgsaa exhibited differential gene expression ... | 2015 | 26523984 |
| biological control of aeromonas salmonicida subsp. salmonicida infection in rainbow trout (oncorhynchus mykiss) using aeromonas phage pas-1. | the potential control efficacy of aeromonas phage pas-1 was evaluated against aeromonas salmonicida subsp. salmonicida infection in rainbow trout (oncorhynchus mykiss) model in this study. the phage was co-cultured with the virulent a. salmonicida subsp. salmonicida strain as05 that possesses the type iii secretion system (ttss) ascv gene, and efficient bacteriolytic activity was observed against the bacteria. the administration of pas-1 in rainbow trout demonstrated that the phage was cleared f ... | 2015 | 23594036 |
| [multiplication of chloroplasts in acetabulaira under red and blue light (author's transl)]. | 2015 | 4445519 | |
| [simulation studies on the behavior of various automatic diagnostic procedures]. | 2015 | 4617105 | |
| predicting optometry grades from the ocat and preoptometry grades. | 2015 | 4525062 | |
| the effect of azide on phototaxis in chlamydomonas reinhardi. | phototaxis in chlamydomonas reinhardi was specifically inhibited by azide. the effect of azide was rapid and reversible, and did not depend upon the intensity of actinic light. under conditions of completely inhibited phototaxis, azide had no effect on the number of motile cells in the population or on the rate of motility. the effect was not related to changes in oxygen uptake or cellular atp concentration. apparently, a cellular component or process specifically involved in phototaxis is inact ... | 2015 | 4525466 |
| mbo goes to work in the public sector. | 2015 | 4492157 | |
| [spinal infarction due to nucleus pulposus emboli (author's transl)]. | 2015 | 4451034 | |
| dermatitis caused by the bat mite, chiroptonyssus robustipes (ewing), in california. | 2015 | 4472534 | |
| [comparative study of the incidence of a number of diseases in children, vaccinated with akds vaccines with varying antigenic and quantitative composition of the pertussis component]. | 2015 | 4785836 | |
| death of methadone users in the district of columbia. | 2015 | 4790688 | |
| [hazards of electrical equipment]. | 2015 | 4483657 | |
| information processing and automatic diagnosis of circulatory system--introductory remarks from engineer's point of view. | 2015 | 4610203 | |
| [case of goldenhar's syndrome (dysplasia oculo-auricularis)]. | 2015 | 4690016 | |
| effect of nanophyetus salmincola and bacterial co-infection on mortality of juvenile chinook salmon. | the freshwater trematode nanophyetus salmincola has been demonstrated to impair salmonid immune function and resistance to the marine pathogen vibrio anguillarum, potentially resulting in ocean mortality. we examined whether infection by the parasite n. salmincola similarly increases mortality of juvenile chinook salmon oncorhynchus tshawytscha when they are exposed to the freshwater pathogens flavobacterium columnare or aeromonas salmonicida, two bacteria that juvenile salmonids might encounter ... | 2015 | 26671546 |
| diversity and homogeneity among small plasmids of aeromonas salmonicida subsp. salmonicida linked with geographical origin. | furunculosis, which is caused by aeromonas salmonicida subsp. salmonicida, is a major salmonid disease in fish farms worldwide. several plasmids found in this bacterium confer phenotypes such drug resistance and virulence. small plasmids (pasa1, pasa2, pasa3, and pasal1) related to cole1- and cole2-type replicons are usually present in its normal plasmidome. in the present study, with the objective to investigate if these plasmids display particularities related to the origin of the isolates bea ... | 2015 | 26635745 |
| effects of dietary linseed oil on innate immune system of eurasian perch and disease resistance after exposure to aeromonas salmonicida achromogen. | this study was designated to investigate the effects of dietary fish oil (fo diet) replacement by linseed oil (lo diet) on regulation of immune response and disease resistance in eurasian perch (perca fluviatilis). a control diet containing fish oil (fo = cod liver oil) and characterized by high levels of n-3 high lc-pufa (6% epa, 7.5% of total fatty acids (fas)) was compared to linseed oil diet (lo diet) composed of low lc-pufa contents (1% epa, 2.3% dha of total fas) but high c18 fatty acids l ... | 2015 | 26497094 |
| disparate developmental patterns of immune responses to bacterial and viral infections in fish. | during early stages of development vertebrates rely on an immature immune system to fight pathogens, but in non mammalian species few studies have taken an in-depth analysis of the transition from reliance on innate immune mechanisms to the appearance of adaptive immunity. using rainbow trout as a model we characterized responses to two natural pathogens of this species, the gram negative bacterium aeromonas salmonicida and the virus vhsv, using microarray analysis at four early life history sta ... | 2015 | 26487553 |
| aeromonas salmonicida infection only moderately regulates expression of factors contributing to toll-like receptor signaling but massively activates the cellular and humoral branches of innate immunity in rainbow trout (oncorhynchus mykiss). | toll-like receptors (tlrs) are known to detect a defined spectrum of microbial structures. however, the knowledge about the specificity of teleost tlr factors for distinct pathogens is limited so far. we measured baseline expression profiles of 18 tlr genes and associated signaling factors in four immune-relevant tissues of rainbow trout oncorhynchus mykiss. intraperitoneal injection of a lethal dose of aeromonas salmonicida subsp. salmonicida induced highly increased levels of cytokine mrnas du ... | 2015 | 26266270 |
| histopathological findings in farmed rainbow trout (oncorhynchus mykiss) naturally infected with 3 different aeromonas species. | this study describes the macroscopic and microscopic lesions in farmed rainbow trout (oncorhynchus mykiss) naturally infected with genetically identified aeromonas salmonicida, a. hydrophila, and a. veronii species. the genus aeromonas includes bacteria that naturally inhabit both waterways and organisms. at least 27 aeromonas species have been identified to date, some of which can cause significant economic losses in aquaculture. as up to 68.8% of aeromonas isolates may be misidentified in rout ... | 2015 | 26130859 |
| effect of a phytogenic feed additive on the susceptibility of onchorhynchus mykiss to aeromonas salmonicida. | in recent years, feed additives have increasingly been adopted by the aquaculture industry. these supplements not only offer an alternative to antibiotics but have also been linked to enhanced growth performance. however, the literature is still limited and provides contradictory information on their effectiveness. this is mainly due to the wide variety of available products and their complex mechanisms of action. phytogenic feed additives have been shown to have antimicrobial effects and can im ... | 2015 | 26119300 |
| functional genomics of the aeromonas salmonicida lipopolysaccharide o-antigen and a-layer from typical and atypical strains. | the a. salmonicida a450 lps o-antigen, encoded by the wbsalmo gene cluster, is exported through an abc-2 transporter-dependent pathway. it represents the first example of an o-antigen lps polysaccharide with three different monosaccharides in their repeating unit assembled by this pathway. until now, only repeating units with one or two different monosaccharides have been described. functional genomic analysis of this wbsalmo region is mostly in agreement with the lps o-antigen structure of acet ... | 2015 | 26082990 |
| asagei2b: a new variant of a genomic island identified in the aeromonas salmonicida subsp. salmonicida jf3224 strain isolated from a wild fish in switzerland. | aeromonas salmonicida subsp. salmonicida is the causal agent of furunculosis in salmonids. we recently identified a group of genomic islands (asagei) in this bacterium. asagei2a, one of these genomic islands, has almost exclusively been identified in isolates from north america. to date, aeromonas salmonicida subsp. salmonicida jf3224, a strain isolated from a wild brown trout (salmo trutta) caught in switzerland, was the only european isolate that appeared to bear asagei2a. we analyzed the geno ... | 2015 | 26048417 |
| steelhead trout oncorhynchus mykiss metabolic rate is affected by dietary aloe vera inclusion but not by mounting an immune response against formalin-killed aeromonas salmonicida. | the oxygen consumption (mo2) of two groups of 10° c acclimated steelhead trout oncorhynchus mykiss was measured for 72 h after they were given a 100 µl kg(-1) intraperitoneal injection of formalin-killed aeromonas salmonicida (asal) or phosphate-buffered saline (pbs). in addition, plasma cortisol levels were measured in fish from both groups prior to, and 1 and 3 h after, they were given a 30 s net stress. the first group was fed an unaltered commercial diet for 4 weeks, whereas the second group ... | 2015 | 26010230 |
| growth inhibition of aeromonas salmonicida and yersinia ruckeri by disinfectants containing peracetic acid. | peracetic acid (paa) is a therapeutic agent used for disinfection in aquaculture, but it must be investigated thoroughly in order to mitigate diseases without harming the fish. successful disinfectants (like paa) should not leave dangerous residues in the environment in order to successfully contribute to sustainable aquaculture. the aim of our study was to compare the effectiveness of 6 commercial paa products with different molecular paa:h2o2 ratios to reduce bacterial growth of aeromonas salm ... | 2015 | 25850398 |
| transcriptomic and proteomic analyses of splenic immune mechanisms of rainbow trout (oncorhynchus mykiss) infected by aeromonas salmonicida subsp. salmonicida. | furunculosis caused by aeromonas salmonicida subsp. salmonicida is an epidemic disease among salmonids, including rainbow trout (oncorhynchus mykiss). however, the immune mechanisms that are elicited in rainbow trout against the invasion of a. salmonicida are not yet fully understood. in this study, we examined the spleen to investigate the immune response of rainbow trout at 3days post-infection by a. salmonicida at the transcriptome and proteome levels by using illumina-seq and itraq methods, ... | 2015 | 25849251 |
| molecular characterization of shewanella and aeromonas isolates associated with spoilage of common carp (cyprinus carpio). | storage in ice is a common way of preserving commercial fish species but some microorganisms can still contaminate and participate in the spoilage of the product; therefore, identification of potential harmful microbes is important. thirteen colonies were isolated from common carp (cyprinus carpio) that had been stored in ice, whose phenotypic identification revealed that they belonged to the genera aeromonas (n = 5) and shewanella (n = 8). molecular genotyping with eric-pcr showed clonality onl ... | 2015 | 25790506 |
| comparative evaluation of infection methods and environmental factors on challenge success: aeromonas salmonicida infection in vaccinated rainbow trout. | when testing vaccine-induced protection an effective and reliable challenge method is a basic requirement and we here present a comparative study on different challenge methods used for infection of rainbow trout oncorhynchus mykiss with aeromonas salmonicida, a bacterial pathogen eliciting furunculosis. fish were vaccinated with three different adjuvanted trivalent vaccines containing formalin killed a. salmonicida, vibrio anguillarum o1 and o2a. these were 1) the commercial vaccine alpha ject ... | 2015 | 25783001 |
| improving survival and storage stability of bacteria recalcitrant to freeze-drying: a coordinated study by european culture collections. | the objective of this study is to improve the viability after freeze-drying and during storage of delicate or recalcitrant strains safeguarded at biological resource centers. to achieve this objective, a joint experimental strategy was established among the different involved partner collections of the embarc project ( www.embarc.eu ). five bacterial strains considered as recalcitrant to freeze-drying were subjected to a standardized freeze-drying protocol and to seven agreed protocol variants. ... | 2015 | 25773973 |