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rapid oxidase method for testing oxidase-variable aeromonas hydrophila strains.a rapid, same-day oxidase test procedure which obviates the problem of false-negative oxidase reactions of aeromonas hydrophila removed from the surface of differential media such as macconkey agar is described. this method allows oxidase testing to be performed within 3 h, rather than delaying the oxidase test for an additional 18 to 24 h. this procedure is applicable to any rapidly growing gram-negative rod.19817019239
travelers' diarrhea among american peace corps volunteers in rural thailand.diarrheal disease was studied prospectively in 35 peace corps volunteers during their first five weeks in rural thailand. twenty (57%) developed the syndrome of travelers' diarrhea. recognized bacterial enteric pathogens were isolated from stools during 47% of 39 episodes of travelers' diarrhea. enterotoxigenic escherichia coli was isolated during 26% and shigella during 13% of the episodes. of the 20 volunteers, 50% had bacteriologic and/or serologic evidence of infection with enterotoxigenic e ...19817019355
medium for the accumulation of extracellular hemolysin and protease by aeromonas hydrophila.a medium for the accumulation of extracellular hemolysin (300 to 1,600 hemolytic units per ml) and protease (2 to 3 proteolytic units per ml) was developed for an anaerogenic strain of aeromonas hydrophila. in this medium, growth yields were less but levels of accumulated toxin were greater or equivalent when compared with the same responses in brain heart infusion and nutrient broths. the medium was considered to be partially defined since the conditions for maximum observed hemolysin accumulat ...19817026444
[infections caused by aeromonas hydrophila. review of their clinical and bacteriological aspects]. 19817029675
toxin-producing bacteria in infants. lack of an association with sudden infant death syndrome.after finding enterotoxigenic (et) escherichia coli in two consecutive cases of sudden infant death syndrome (sids), we initiated a prospective search for et e coli in sids in manitoba; in addition, we looked for toxigenic clostridium botulinum and c difficile. in a 21-month period, small- and large-bowel contents were obtained in 33 cases of sids, from 32 control subjects, and in ten suspected cases of sids. neither c botulinum nor et e coli was isolated from any of these; c difficile was cultu ...19817032280
extracellular virulence factors of aeromonas hydrophila in fish infections.aeromonas hydrophila nrc 505 produced extracellular substances which were capable of causing pathological effects when injected into trout. proteolytic activity and haemolytic activity of the extracellular products, and the effect on fish, were lost on heating. the extracellular substances from strain g35, a protease-deficient mutant, were significantly more toxic to both rainbow trout (salmo gairdneri) and speckled trout (salvelinus fontinalis) than the analogous preparation from the parental s ...19817032678
evaluation of a test-kit (oxi/ferm system) for identification of vibrios.a total of 258 strains belonging to different species of vibrio isolated from various sources in different parts of the world were subjected to identification by oxi/ferm tube and conventional tests sometimes extending to 250 characters when the conventional tests failed to recognise them. nearly 88 per cent of them could be identified accurately in this system at the species level. the strains designated as mannitol negative vibrios and vibrio species were also identified properly as v. cholera ...19817036597
microbial flora of the lower genital tract of women in labour in zaria, nigeria.nine genera of microbes isolated from the lower genital tract of 187 women in labour in zaria have been identified. the work was undertaken to establish the nature of microorganisms in the lower genital tract of women in labour as a basis for further study. the isolates in order of prevalence were: candida albicans (20.9%), klebsiella sp (15.0%), escherichia coli (9.1%), streptococcus faecalis (6.4%), haemolytic streptococci (other than streptococcus pyogenes (2.7%), streptococcus viridans (2.1% ...19817007447
bacteria associated with false-positive most-probable-number coliform test results for shellfish and estuaries.aerobic and facultatively anaerobic bacteria isolated from false-positive, presumptive, total coliform, most-probable-number tests of chesapeake bay oyster, water, and sediment samples were characterized and then classified by numerical taxonomy. a total of 538 bacterial strains clustered into 17 phena, the predominant groups of which were enterobacteriaceae (including escherichia coli), aeromonas spp., and bacillus spp. bacillus spp. were recovered most frequently from sediment samples. gas-pro ...19817013700
enterotoxigenic bacteria in food and water from an ethiopian community.food and water samples from an ethiopian community were screened for the presence of enterotoxin-producing bacteria. using the chinese hamster ovary cell assay, 40 of 213 isolates (18.8%) produced heat-labile (lt) enterotoxin. these lt-producing isolates comprised 33 of 177 (18.6%) strains from 24 of 68 food samples (35.3%) and 7 of 36 (19.4%) isolates of 4 of 17 water samples (23.5%). one lt-producing strain each of salmonella emek and of shigella dysenteriae was found. three pseudomonads, all ...19817016032
increased resistance to aeromonas hydrophila in mice experimentally infected with trypanosoma cruzi. 19816790693
purification and some properties of the hemolytic toxin aerolysin.aerolysin, the hemolytic toxin produced by aeromonas hydrophila, has been purified by a combination of salt fractionation, gel filtration, and ion-exchange and hydroxyapatite chromatography. the resulting protein has a molecular weight of 51 500 and appears homogeneous by polyacrylamide gel electrophoresis in sodium dodecyl sulphate. it is free of detectable protease and phospholipase activities. the purified protein can be separated into two active components with pis of 5.39 and 5.46 by isoele ...19816794885
comparison of three microbial toxicity screening tests with the microtox test. 19816802203
taxonomy and description of vibrio fluvialis sp. nov. (synonym group f vibrios, group ef6). 19816971864
aeromonas hydrophila in wild-caught frogs and tadpoles (rana pipiens) in minnesota.frogs and tadpoles were captured at 14 sites in and near minnesota during 1978-79 and nearly all appeared healthy. aeromonas hydrophila was isolated from 94 of 294 (32%) juvenile and adult frogs and from 66 of 104 (63%) tadpoles. of the isolates from frogs and tadpoles respectively, 68% and 47% were from the intestine only, 12% and 32% were from the intestine and the other sites, and 20% and 21% were from extraintestinal sites only. isolations were more frequent from frogs collected in march-jun ...19816972468
bacterial survival in a dilute environment.bacteria were isolated from lake water, and their ability to remain viable in a dilute, nutrient-deficient environment was tested by a method that permits suspension of test bacteria between two appressed microporous membranes in an aqueous environment. this approach permitted separation of the lake isolates into two categories. members of the tribe klebsielleae were shown to have a prolonged survival rate of 40% or better after 24 h, whereas nonsurvivors were not viable for much longer than 24 ...19816454390
viruses and bacteria in pediatric diarrhea in thailand: a study of multiple antibiotic-resistant enteric pathogens.children with diarrhea admitted to a rehydration ward of a children's hospital in bangkok were investigated to determine the prevalence of enteric pathogens, the extent of children's previous antibiotic therapy, and the frequency of plasmid-mediated antibiotic resistance among infecting bacteria. rotavirus (36%), enterotoxigenic escherichia coli (18%), shigella (9%), salmonella (6%), campylobacter jejuni/coli (4%), and vibrio cholerae (2%) infections were among 105 children with diarrhea. antibi ...19816275727
elevation of adenosine 3' 5' cyclic monophosphate level by aeromonas hydrophila enterotoxin. 19816281180
association of aeromonas sobria with human infection.fifteen aeromonas isolates from various human infections and nine isolates from polluted water were identified as either aeromonas hydrophila or aeromonas sobria and examined for cytotoxigenicity, enterotoxigenicity, adherence to epithelial cells, and other virulence-associated factors, including proteases, lipases, elastases, and hemolysins. two groups of organisms (i and ii) were distinguishable based on differences in median lethal doses in mice and cytotoxicity for y-1 adrenal cells. group i ...19816112237
aeromonas bacteraemia in a burn patient. 19816117752
clustering of aeromonas hydrophila septicaemia. 19816118660
aeromonas hydrophila infection of the skin. 19817212750
effects of pharmaceutical wastes on microbial populations in surface waters at the puerto rico dump site in the atlantic ocean.a series of cruises during 1979 and 1980 to the pharmaceutical dump site located 64 km north of arecibo, puerto rico, in the atlantic ocean, was carried out to evaluate effects of wastes on the ecology of the microflora of surface waters of the dump site. in addition to bacteriological monitoring of the waste plume created by the release of wastes from the disposal barge, stations along a series of transects, extending north from coastal waters through and beyond the dump site, were sampled. lar ...198116345752
nonselectivity of rimler-shotts medium for aeromonas hydrophila in estuarine environments.thirty presumptive aeromonas hydrophila isolates were collected from estuarine sources using rimler-shotts media. many of the isolates, especially the strains from a low-salinity site, were also identified as a. hydrophila using the api 20e system and two differential techniques devised for the identification of a. hydrophila. all of the isolates, however, had deoxyribonucleic acid base compositions with guanine-plus-cytosine ratios between 40 and 49 mol%, which excludes these strains from the g ...198116345850
external microflora of a marine wood-boring isopod.bacteria associated with the marine wood-boring isopod limnoria lignorum were enumerated by acridine orange epifluorescence microscopy and by plate counts on several media; the plate-viable bacteria were isolated and identified. similar procedures were followed to enumerate and identify bacteria associated with the wood substrate from which the isopods were collected and with the surrounding water from the isopod habitat. approximately 1.4 x 10 bacterial cells were associated with each individua ...198116345871
diversity dynamics of marine bacteria studied by immunofluorescent staining on membrane filters.of 34 strains of marine bacteria isolated on a general seawater medium, 5 were selected for detailed studies of their population dynamics in the plankton. the isolates were characterized as aeromonas sp., chromobacterium cf. lividum, vibrio sp., and two pseudomonas spp. specific antibodies were produced by immunization of rabbits, and bacterial cells were stained on black uni-pore membrane filters by an indirect immunofluorescent staining procedure. the method proved to be very specific and prac ...198216345920
isolation and characterization of ultramicrobacteria from a gulf coast estuary.viable bacteria were recovered from estuarine waters passed through a 0.2-mum polycarbonate membrane filter. the recovery method included the use of a dilute nutrient broth for primary enrichment followed by conditioning of the organism to a dilute nutrient solid medium. these bacteria were gram-negative rods and coccobacilli having an nacl requirement and, upon initial culturing, low nutritional requirements. in response to increased nutrient preparations, these microorganisms underwent an incr ...198216345964
aeromonas species as enteric pathogens. 19826119581
aeromonas hydrophila liver abscess. 19826121985
the use of aerolysin toxin as an aid for visualization of low numbers of african trypanosomes in whole blood.a method was developed for detection of low numbers of african trypanosomes in whole blood. the method is based on selective lysis of erythrocytes and leukocytes by aerolysin, a toxin produced by the bacterium aeromonas hydrophila. african trypanosomes do not bind the toxin and their viability and motility are therefore unaffected by this treatment.19826122365
enteropathogenicity: recent developments.recent studies have added important new information to our understanding of the pathogenesis and ethiology of diarrheal disease. vibrio cholerae produces a heat-labile enterotoxin, affecting cyclic amp. a very similar heat-labile enterotoxin is produced also by certain strains of escherichia coli, as well as by citrobacter, klebsiella, and aeromonas. e. coli may also produce a heat-stable enterotoxin, stimulating guanylate cyclase activity. in order to produce the pathologic effects, e. coli fir ...19826126611
aeromonas-associated gastroenteritis.enterotoxigenic aeromonas spp. were isolated from 118 (10.2%) children with diarrhoea and 7 (0.6%) of those without diarrhoea in a prospective, year-long study of 1156 children with gastroenteritis and the same number of age and sex matched controls. in perth, western australia, aeromonas-associated diarrhoea is distinctly seasonal with a sharp summer peak. the disease most commonly presents in children under two years of age as watery diarrhoea of short duration and mild fever which require no ...19826128598
the effect of primary and secondary immunization on the lymphoid tissues of the carp, cyprinus carpio l.mirror carp immunized with human gamma globulin (hgg) in freund's complete adjuvant (fca) show a proliferative response involving cells whose cytoplasm stains deep red with methyl green-pyronin (pyroninophilic cells). this response occurs particularly in the haemopoietic parenchyma of the pronephros and mesonephros. it peaks at week 3, with the formation of clusters of pyroninophilic cells in the pronephros. immunization with aeromonas salmonicida elicited a less intense pyroninophilic response ...19826177828
in vitro activity of norfloxacin, a quinolinecarboxylic acid, compared with that of beta-lactams, aminoglycosides, and trimethoprim.norfloxacin is a quinolinecarboxylic acid compound. we examined the in vitro activity of this compound against gram-positive and -negative species, including anaerobic species. it inhibited 90% (mic90) of strains of escherichia coli at 0.05 microgram/ml, klebsiella sp. at 0.4 microgram/ml, salmonella and shigella spp. at 0.1 microgram/ml, citrobacter sp. at 0.4 microgram/ml, enterobacter cloacae at 0.2 microgram/ml, enterobacter aerogenes at 0.4 microgram/ml, and enterobacter agglomerans at 0.2 ...19826214995
[complex studies in the surveillance of the environmental vibrio flora in the city of gorki and gorki province].the analysis of the results of the comprehensive studies (phenotypic and genotypic properties, the precipitation test with the use of spontaneously agglutinating cultures, the determination of the antigenic structure by immunochemical analysis, etc.) carried out during the sanitary microbiological control of the environmental vibrioflora for the last 5 years are presented. only the combined use of the above-mentioned methods has allowed to ascertain the taxonomic position of atypical cultures.19826218723
[antibacterial activities of cefotiam against various pathogens isolated from clinical materials (author's transl)].bacteriological evaluation was made on cefotiam (ctm), a new broad-spectrum cephalosporin antibiotic, and the following results wer obtained. 1) ctm has shown very potent antibacterial activities against staphylococcus aureus, escherichia coli, edwardsiella tarda, citrobacter intermedius, salmonella, klebsiella, proteus mirabilis, proteus rettgeri, proteus inconstans, yersinia enterocolitica, aeromonas hydrophila, plesiomonas shigelloides and pseudomonas putrefaciens. 2) streptococcus faecalis, ...19826285018
[near-drowning in an adult: favorable course after a 20-minute submersion].cardiopulmonary resuscitation was successful in a healthy 29-year-old woman who had been submerged for 20 minutes in water at 10 degrees c. the evolution was characterized by the development of a multifactorial ards (secondary drowning) and sepsis caused by aeromonas hydrophila and acinetobacter anitratum. fibrosing alveolitis caused a restrictive syndrome with severe mechanical impairment and transitory therapy-resistant hypoxemia. it is suggested that prolonged submersion in cold water is also ...19826287569
[comparison of antimicrobial activity and stability to beta-lactamases of cefoperazone, cefotaxime, lamoxactam and ceftriaxon].ceftriaxon (ro 13-9904) is a new cephalosporin, whose structure resembles cefotaxime. this study compares the minimal inhibitory concentrations (mic-s) of ceftriaxon, cefoperazone, cefotaxime, lamoxactam and gentamicin against 622 isolates of various enterobacteriaceae, non-fermenters and aeromonas hydrophila. furthermore the minimal bactericidal concentrations (mbc-s) were determined for some organism; additionally destruction of beta-lactamantibiotics in the presence of beta-lactamases was stu ...19826289564
the effect of n-formimidoyl thienamycin, ceftazidime, cefotiam, ceftriaxone and cefotaxime on non-fermentative gram-negative rods, aeromonas, plesiomonas and enterobacter agglomerans.thirty-one species (185 strains) of non-fermentative gram-negative rods (excluding pseudomonas aeruginosa) as well as 45 strains of aeromonas spp., 15 strains of plesiomonas shigelloides and 68 strains of enterobacter agglomerans were tested in microdilution procedures against n-formimidoyl thienamycin, ceftazidime, cefotiam, ceftriaxone and cefotaxime. n-formimidoyl thienamycin was the most effective drug as far as the spectrum of these bacterial groups and potency is concerned; ceftazidime was ...19826293976
enzymes of arginine utilization and their formation in aeromonas formicans ncib 9232.in aeromonas formicans two inducible catabolic pathways of l-arginine have been characterized. the arginine decarboxylase is induced by arginine which also induces the three enzymes of the arginine deiminase pathway but only in stress conditions such as a shift from aerobic growth conditions to very low oxygen tension. addition of glucose to medium containing arginine leads to repression of the enzymes involved in the arginine deiminase pathway while exogenous camp prevents that repression of en ...19826303241
[antibiotic sensitivity and other properties of bacteria of the genus aeromonas].antibiotic sensitivity and other biological properties of aeromonas and nad-vibrios isolated from water of open waterbodies were studied comparatively. it was found that the organisms of these genera had a definite similarity with respect to antibiotic sensitivity, morphological, cultural and some antigenic properties. the differences in the antibiotic sensitivity of these organisms were not sufficient for definition of the taxonomic features.19826460468
[aeromonas hydrophila as a causative agent of diarrhea]. 19826982424
aeromonas hydrophila infection: ecthyma gangrenosum with aplastic anemia. 19826959002
biochemical characteristics of enterotoxigenic aeromonas spp.biotypes of aeromonas spp. correlated well with enterotoxin production in a study of 174 strains. using biochemical characteristics determined by conventional methods and multitest systems, we correctly classified 93% of the strains with regard to enterotoxin production. most of the enterotoxigenic strains were voges-proskauer (vp) positive and did not hydrolyze arabinose, but vp-positive strains which hydrolyzed arabinose were mainly non-enterotoxigenic. aeromonas punctata subsp. caviae, which ...19826764776
cytotonic enterotoxin from aeromonas hydrophila.aeromonas hydrophila produces two hemolysins and an enterotoxin during growth. enterotoxin, separated from the hemolysins, gave positive reactions in the rabbit intestinal loop test, the rabbit skin test and the adrenal y1 cell test. neutralization experiments in the rabbit loop, rabbit skin and y1 cell tests failed to demonstrate any immunological relationship between aeromonas enterotoxin and cholera toxin or escherichia coli heat-labile enterotoxin. prior incubation of aeromonas enterotoxin w ...19826753242
characterization of indicator bacteria in municipal raw water, drinking water, and new main water samples.municipal water samples were analyzed by membrane filter (mf) and presence-absence (p-a) tests for pollution indicator bacteria. in four years, 11 514 bacterial cultures were isolated from either raw water, drinking water, or new main water samples submitted to three environmental laboratories. the bacterial species occurring most often in all types of water samples were escherichia coli (11.6-39.7%), enterobacter aerogenes (18.1-26.3%), aeromonas hydrophila (8.8-17.0%), klebsiella pneumoniae (7 ...19826754044
thin layer iso-electro focusing of intracellular bacterial protein in classification of some representatives of the families enterobacteriaceae and vibrionaceae.intracellular bacterial protein from some representatives of the families enterobacteriaceae and vibrionaceae has been investigated using thin layer iso-electric focusing in polyacrylamide gel. a marked difference between the protein patterns was observed between species within the same family. carrying out the iso-electric focusing at ph range 3.5-9 gave a large number of protein bands. increased resolution of the protein bands was obtained using iso-electric focusing at the ph range 4-6.5. car ...19826756032
cholera-like illness due to aeromonas sobria.a thai woman from bangkok was admitted to a hospital in paris for a cholera-like illness. a culture of her "rice-water" diarrhea was negative for vibrio cholerae and enterotoxigenic escherichia coli but was positive for aeromonas sobria. this strain produced enterotoxin, cytolysin, proteolysin, hemolysin, and a cell-rounding factor. acute -and convalescent-phase sera showed an increase in neutralizing antibodies to enterotoxin, cytolysin, and hemolysin. the enterotoxin, which was labile at 100 c ...19827054327
cell surface of the fish pathogenic bacterium aeromonas salmonicida. i. relationship between autoagglutination and the presence of a major cell envelope protein.a comparison was made of membrane protein patterns of various aeromonas salmonicida strains, initially isolated from different habitats with respect to fish species affected, pathological entity, and geographic location of the outbreak of the disease. a major protein with a molecular weight of 54 000 was found in all autoagglutinating strains, whereas this protein is present in low amounts, or not at all, in non-autoagglutinating strains. evidence for a causal relationship between the presence o ...19827055565
cell surface of the fish pathogenic bacterium aeromonas salmonicida. ii. purification and characterization of a major cell envelope protein related to autoagglutination, adhesion and virulence.the purification of the major protein of the membrane fraction of an autoagglutinating strain of aeromonas salmonicida is described. this protein, designated as additional cell envelope protein, is water-insoluble, has a molecular weight of about 54 000 and its amino terminal sequence is h2n-asp-val-leu-leu. neither sulphur-containing amino acids nor sugar residues were detected. its amino acid composition, which shows that the additional cell envelope protein is hydrophobic in nature, is remark ...19827055566
enteropathogenicity of aeromonas hydrophila and plesiomonas shigelloides: prevalence among individuals with and without diarrhea in thailand.to evaluate the enteropathogenicity of aeromonas hydrophila and plesiomonas shigelloides, the rate of isolation of these organisms was compared among individuals with and without diarrhea in thailand. in two groups of american travelers, a. hydrophila, but not p. shigelloides, was associated with episodes of travelers diarrhea more often than when individuals did not have diarrhea (p less than 0.025). among three populations of thais, a. hydrophila and p. shigelloides were isolated with similar ...19827056580
photochemical inactivation of aeromonas aminopeptidase by 2,3-butanedione. 19827056743
purification and partial characterization of a bacterial phospholipid: cholesterol acyltransferase.a glycerophospholipid:cholesterol acyltransferase has been purified to near homogeneity from cell-free culture supernatants of aeromonas salmonicida. the characteristics of the enzyme distinguish it from bacterial phospholipases; however, it shares several properties with the lecithin:cholesterol acyltransferase of mammalian plasma. thus, the enzyme inhibits 2-positional specificity as an acyltransferase and it will act as a phospholipase a2 in the absence of cholesterol. furthermore, it has no ...19827061477
oxidation, photosensitized by certain diketones, of enzymes and protection against such oxidation by histidine derivatives.bovine milk lactoperoxidase, eel acetylcholinesterase, and aeromonas aminopeptidase were photooxidized and inactivated in broad-spectrum visible light in the presence of 2,3-butanedione and 1-phenyl-1,2-propanedione. methylglyoxal caused similar effects at 254 nm. 2-thiol-l-histidine and 3-methyl-l-histidine protected the enzymes against photoinactivation more effectively then n3-, even at a molar ratio of 2:1 (protector to enzyme). these compounds also delayed the photoinactivation of acetylcho ...19827066488
aeromonas hydrophilia: a rare and potentially life-threatening pathogen to humans.the authors discuss the diagnosis, symptoms, and treatment of infections resulting from aeromonas hydrophilia, and uncommon pathogen that is being reported with increasing frequency and may be serious to man. they present the case history of a 38-year-old male and emphasize the importance of prompt and proper treatment.19827069142
[aeromonads in food products]. 19827076001
two membrane damaging toxins from aeromonas hydrophila.the effects of two hemolysins (alpha and beta) from aeromonas hydrophila on human lung fibroblasts were investigated. the toxins differed with respect to relative size of lesions induced in the plasma membrane, morphological effects, reversibility of effects and adsorption to fibroblasts. the effect of alpha-hemolysin appears to be restricted to the plasma membrane level, whereas the beta-hemolysin is strongly cytotoxic and probably acts on some intracellular target in addition to the primary ta ...19827080029
aeromonas hydrophila toxins - intestinal fluid accumulation and mucosal injury in animal models.strains of aeromonas hydrophila were earlier shown to produce a heat-labile enterotoxin and two cytolytic toxins, alpha- and beta-haemolysin. these investigations have been extended. when separated from the cytolysins, the enterotoxin elicited fluid accumulation in rabbit intestinal loops. the electrolyte and albumin content of the fluid was similar to that of cholera toxin. the enterotoxin was non-injurious to the mucosa. purified hemolysins did not give a positive loop test. they produced hemo ...19827080051
membrane glycoprotein receptor and hole-forming properties of a cytolytic protein toxin.aerolysin, a cytolytic bacterial exotoxin, was radioiodinated by using the iodogen reagent. binding of the labeled toxin to rat erythrocytes was inhibited by the native protein and by anti-aerolysin antibody. toxin, once bound, was not removed by the addition of a large excess of free aerolysin. binding of the radioactive toxin to erythrocytes of different species paralleled the hemolytic specificity of the unlabeled toxin. pretreatment of the rat erythrocytes with trypsin, which removed a major ...19827082638
aeromonas food poisoning in nigeria: a case report. 19827083319
in-vitro susceptibility of aeromonas app. to antimicrobial agents. 19827085531
diketones as photosensitizing agents: application to alpha-amino acids and enzymes. 19827089079
role of surface components in serum resistance of virulent aeromonas salmonicida.the ability of virulent strains of aeromonas salmonicida to resist the bactericidal activity of serum was quantitated. the a. salmonicida strains tested included virulent strains, mutants lacking the major surface a-protein, and mutants lacking a-protein and having a modified lipopolysaccharide structure. the sera evaluated included normal human, rabbit, and trout sera, immune trout serum, and immune rabbit serum containing antibodies to a-protein and lipopolysaccharide. virulent strains of a. s ...19827095843
purification and characterization of a novel exo-beta-mannanase from aeromonas sp. f-25.a novel exo-beta-mannanase (1,4-beta-d-mannan mannobiohydrolase) was isolated from the culture fluid of strain no. f-25 of aeromonas hydrophila subspecies anaerogenes, and purified about 4,000-fold by ammonium sulfate precipitation and successive co.umn chromatographies. the final enzyme preparation appeared to be homogeneous on polyacrylamide gel electrophoresis. the enzyme hydrolyzed the beta-1,4-mannan link in polysaccharides of three or more beta-1,4-linked d-mannose units. the enzyme had a ...19827096283
hand carriage of aerobic gram-negative rods by health care personnel.a quantitative culture technique (hand washed in a glove containing broth for 30 s) was used to determine the frequency of hand carriage of aerobic gram-negative rods by various groups of health care workers and 104 control subjects. overall, 31% of health care workers carried aerobic gram-negative rods on their hands compared to 59% of control subjects (p < 0.001). enterobacter agglomerans accounted for 40% of the isolates, and other enterobacter spp. 7%. other organisms included acinetobacter ...19827097001
chromium trioxide oxidation of acetylated 3-amino-3,6-dideoxy-l-hexosides.glycosides of the fully acetylated 3-amino-3,6-dideoxy-l-hexosides in the gluco, galacto, manno, and talo configurations have been treated with chromium trioxide in acetic acid. the alpha-l-methyl glycosides, which exist in the more stable chair conformation 1c4, with an axially oriented aglycon, are resistant to this oxidation, whereas the beta-l-linked glycoside having the gluco configuration (1c4 conformation) and the alpha-l-methyl glycoside having the talo configuration (4c1 conformation), ...19827104829
numerical taxonomy of psychrotrophic pseudomonads.the taxonomy of 218 psychrotrophic pseudomonad strains (200 field strains from meat and 18 type and reference strains) was numerically studied by 174 biochemical and physiological tests. all strains were gram-negative rods, oxidative positive and motile by means of one or more polar flagella. the strains clustered into 15 groups, of which 9 were regarded as major clusters. the major clusters were designated as pseudomonas fragi (112 strains), p. fluorescens biotype iii (7 strains), p. fluorescen ...19827119735
quality control and interpretive criteria for the azlocillin disk diffusion susceptibility test.the standardized disk diffusion test was performed with 75-micrograms azlocillin disks to determine individual test, accuracy, and precision control values with escherichia coli atcc 25922, pseudomonas aeruginosa atcc 27853, and staphylococcus aureus atcc 25923. in addition, regression lines for correlating inhibitory zone diameters with the 75-micrograms azlocillin disk and azlocillin minimal inhibitory concentrations were calculated for gram-negative clinical isolates (including enterobacteria ...19827125631
diluent composition for use of api 20e in characterizing marine and estuarine bacteria.nine chemically defined inoculation diluents, with compositions ranging from 0.85% nacl to 35% marine salts, were used to evaluate the influence of diluent composition on the biochemical profiles of 30 marine and estuarine bacterial strains, including species of vibrio, aeromonas, allomonas, and photobacterium. results demonstrated that a 20% marine salts diluent enabled the characterization of halophilic strains normally nonreactive by the api 20e system. furthermore, the use of 20% marine salt ...19827125655
in vitro susceptibilities of aeromonas hydrophila against new antibiotics.the antibiotic susceptibilities of 16 clinical isolates of aeromonas hydrophila obtained from cancer patients with septicemia were studied. of the new beta-lactam antibiotics tested, azthreonam and moxalactam were the most active, followed by cefoperazone, cefotaxime, and ceftizoxime. excellent activity was demonstrated by chloroamphenicol, tetracycline, aminoglycosides, and trimethoprim-sulfamethoxazole. semisynthetic penicillins had no appreciable activity against this organism.19827137987
aeromonas hydrophila infection complicating an open tibial fracture. a case report.aeromonas hydrophila is a gram-negative bacterium that commonly inhabits soil and stagnant water. on extremely rare occasions, the organism can cause an aggressive, rapidly spreading, necrotizing infection in humans. the systemic signs of high fever, tachycardia, and elevation of the white blood cell count appear within 24 hours of wound contamination. if the wound is obscured by a cast, these signs erroneously may be attributed to a respiratory problem, e.g., atelectasis or fat metabolism. the ...19827140058
ecology and importance of bacterial' species of the family vibrionaceae.the bacterial species of the family vibrionaceae, particularly the species of the genus aeromonas (a. hydrophila and a. punctata and their subspecies were found to be relatively common in the running surface water in czechoslovakia (the occurrence of a. salmonicida species was not studied). the strains of the so-called nag-vibrios (vibrio cholerae unrelated to the cholera vibrio 0 group 1) were also relatively frequent, but the positive isolates of plesiomonas shigelloides were sporadic. the abo ...19827142689
[gas gangrene caused by aeromonas hydrophila. a therapeutic trap]. 19827145671
isolation of chromobacterium spp. from foods, soil, and water.chromobacterium violaceum, a soil and water inhabitant, has been implicated in human disease with a high mortality rate, particularly in the southeastern united states. the psychrotrophic chromobacterium lividum has been isolated from foods, water, and soil, but is not considered pathogenic. to determine the distribution of chromobacterium spp. in soil, water, and foods in the gainesville area, we evaluated bennett, ryalls and moss, and aeromonas membrane agars for their ability to recover these ...19827159087
substrate specificity of bacterial glycerophospholipid:cholesterol acyltransferase.the substrate specificity of a bacterial analogue of the plasma enzyme lecithin:cholesterol acyltransferase (lcat) has been examined with small unilamellar liposomes and triton mixed micelles. in contrast to lcat, the microbial enzyme is capable of using all of the naturally occurring phospholipids as acyl donors. in general reaction rate depends more on the length or degree of unsaturation of the acyl chains than on the nature of the phospholipid head group. among a series of disaturated phosph ...19827159555
molluscan immunobiology: isolation of an aeromonas formicans which escapes the internal defense system of helix pomatia.bacterial infections are surprisingly rare in wild populations of gastropod molluscs. a strain of aeromonas formicans, which has now been isolated, kills helix pomatia, a land snail, when injected at doses greater than 10(6) viable cells g-1 snail weight. koch's postulates were fulfilled with this organism. initial clearance of aeromonas from the circulation was followed within hours by a bacteremia; since neither culture supernatants, killed cells nor cell extracts killed helix, there is eviden ...19827160512
gas gangrene: an unusual manifestation of aeromonas infection. 19827161594
enumeration and identification of pathogenic bacteria from sewage in kuwait.mycobacterium spp. was isolated and enumerated in all sewage samples at different stages of the sewage treatment. aeromonas hydrophila, salmonella spp., shigella dysenteriae and others were isolated from both treated and untreated sewage. moreover, the serological identification of salmonella spp. was also done, using antisera specific to their antigenic structures. counts of bacteria in various sewage sludges and effluents tested showed the efficiency of the activated sludge process in reducing ...19827168261
occurrence of uronic acid in lipopolysaccharides of vibrionaceae.the occurrence of uronic acid as a sugar constituent of lipopolysaccharides (lps) in vibrionaceae was demonstrated for the first time. more than 100 strains were examined. of five genera constituting vibrionaceae, i.e., vibrio, aeromonas, plesiomonas, photobacterium, and lucibacterium, the latter three contained uronic acid in lps of all of their constituting members examined, while it was totally lacking in aeromonas lps so far tested. only the members of genus vibrio were found to be divided i ...19827169971
chemical modification of aeromonas aminopeptidase. evidence for the involvement of tyrosyl and carboxyl groups in the activity of the enzyme. 19827173208
plesiomonas (aeromonas) shigelloides meningitis in a neonate--a case report. 19827175386
cellulitis due to aeromonas hydrophilia. 19827175393
sugar composition of lipopolysaccharides of family vibrionaceae. absence of 2-keto-3-deoxyoctonate (kdo) except in vibrio parahaemolyticus o6. 19827176968
aeromonas hydrophila septicemia in a patient with chronic active hepatitis. 19827176998
toxic factors of aeromonas hydrophila and plesiomonas shigelloides.in detail, exoproducts of aeromonas hydrophila and plesiomonas shigelloides were studied on the pathogenesis of diarrhoea. enterotoxin, hemolysin and a cytotoxic factor were found in a fraction of culture filtrates of aeromonas hydrophila with a molecular weight of approximatley 60,000. apart there from a cytotoxic factor was also found in a fraction with molecular weight above 100,000. this fraction contained also endotoxin which was hemolytic, too. enterotoxic activity was expressed by an accu ...19827180235
antibacterial action of combinations of oxytetracycline, dimethyl sulfoxide, and edta-tromethamine on proteus, salmonella, and aeromonas.antibacterial effects against proteus mirabilis, salmonella typhimurium, and aeromonas hydrophila were obtained with subminimal inhibitory concentrations of oxytetracycline and edta-tromethamine. antibacterial effects were not observed with subminimal inhibitory concentrations of dimethyl sulfoxide plus oxytetracycline or with dimethyl sulfoxide plus edta-tromethamine. using a 2-dimensional microtiter checkerboard technique, inhibitory activities of the various combinations of solutions were stu ...19826807142
market fish hygiene in kenya.vibrio parahaemolyticus was isolated from 53 out of 584 samples (9.1%) of market fish. all strains were kanagawa negative and were distributed as follows: sea fish 5 out of 370 samples (1.4%), shellfish 48 out of 214 samles (22.4%). other fish spoilage microflora recovered were: alcaligenes faecalis, pseudomonas aeruginosa, proteus vulgaris, aeromonas spp. and vibrio alginolyticus. total aerobic counts and coliform counts per gram for the lake fish ranged from 2.6 x 10(2) to 6.6 x 10(7) and 10 t ...19826808058
[proteolytic microorganisms in liver products].liver products were studied for the content of proteolytic microorganisms during production and storage. their number was at the lowest level in products of thermal processing (10(5) per g on the average). storage at a refrigerator and room temperature increased this level to the average values of 10(6) to 8.10(6) per g. isolated colonies of proteolytic microorganisms were included in the following genera: pseudomonas, aeromonas, escherichia, alcaligenes, bacillus and staphylococcus. the content ...19826808746
bactericidal serum response of the channel catfish against gram-negative bacteria. 19826813155
enzymatic characterization of aeromonas hydrophila complex by the api zym system.enzymatic characterization of 48 aeromonas hydrophila complex isolates from various sources was determined with the api zym system (analytab products, plainview, n.y.). all isolates lacked valine and cystine aminopeptidases, chymotrypsin, alpha-mannosidase, alpha-fucosidase, alpha-galactosidase, and beta-glucuronidase but possessed caprylate esterase-lipase, leucine aminopeptidase, acid phosphatase, phosphoamidase, and n-acetyl-beta-glucosidase. variability was found in the presence of alkaline ...19826818246
[septicemia due to aeromonas species: clinical and bacteriological aspects]. 19826818304
[sensitivity of aerobia microbial strains responsible for overseas infective diarrheas to two chemical substances: trimethoprime sulfamethoxazole and 5-methyl-oxine]. 19826819896
sensitivity, specificity, and reproducibility of the automicrobic system (with the enterobacteriaceae-plus biochemical card) for identifying clinical isolates of gram- negative bacilli.two independent laboratories tested 1,743 clinical isolates by using the enterobacteriaceae-plus biochemical card in the automicrobic system (ams) and identical standard reference methods. included were 55 isolates representing 11 species that cannot be identified by the enterobacteriaceae-plus biochemical card computer program; 3 or these isolates were incorrectly identified as pseudomonas cepacia. with the other 1,688 isolates, 5% of the ams identifications were considered to be equivocal (pro ...19827040457
immunological cross-reactivity of enterotoxins of aeromonas hydrophila and cholera toxin.pre-incubation with anticholera toxin (act) significantly reduced intestinal secretion induced by cell-free broth preparations of heat-labile toxins (lt) of escherichia coli and aeromonas hydrophila in jejunal perfusion experiments in rats in vivo. pre-incubation with act also prevented cytotoxicity by e. coli lt in the y1 cell culture system. pre-incubation had no effect on cytotoxicity in y1 and l132 cell lines or on haemolytic activity with cell-free preparations of a. hydrophila. in another ...19827047024
microbial spoilage of whole sheep livers.when liver treated with antibiotics to inhibit microbial growth were held at 10 degrees c, the initial high ph (6.4) declined as lactic acid accumulated throughout the storage period of 10 days. the glycogen content also declined, but the glucose concentration in the tissues remained high. when livers were allowed to spoil at 10 degrees c, distinct but variable floras developed within the tissues, in the drip, and on the upper surface. deep-tissue floras were composed of anaerobic and facultativ ...19827201781
characterization of plasmids in bacterial fish pathogen.plasmid profiles of representative fish pathogens, aeromonas salmonicida, aeromonas hydrophila, vibrio anguillarum, pasteurella piscicida, yersinia ruckeri, edwardsiella tarda, and renibacterium salmoninarum, were determined by agarose gel electrophoresis with four different plasmid detection methods. a combination of two methods was required to detect the plasmids present in these strains and to calculate precisely the molecular weights of the plasmids. of 38 strains, 28 harbored one or more pl ...19836822413
evaluation of differential and selective media for isolation of aeromonas and plesiomonas spp. from human feces.we studied nine solid and two liquid media for their suitability to select aeromonas and plesiomonas spp. from human stools, using artificially contaminated samples as well as 254 samples from outpatients with and without diarrhea. media with optimal sensitivity and specificity for aeromonas spp. were alkaline peptone-water, trypticase soy broth with ampicillin, inositol-brilliant green-bile salts agar, dextrin-fuchsin-sulfite agar, xylose-sodium desoxycholate-citrate agar, and pril-xylose-ampic ...19836826700
neonatal septicemia and meningitis due to plesiomonas shigelloides. 19836828345
bacteria in enteric lesions of cattle.thirty-nine species of bacteria were isolated from or demonstrated in the abomasal and small and large intestinal mucosa of 23 adult cattle and 41 calves and identified. the bacteria isolated were related to the gross and microscopical lesions. campylobacters, clostridium perfringens type a, c sordellii, actinobacillus lignieresii, fusobacterium necrophorum, mycobacterium paratuberculosis, aeromonas hydrophila and escherichia coli were all associated with specific lesions. the relationship of ot ...19836829137
the medicinal leech and its use in plastic surgery: a possible cause for infection.recent interest in the use of the medicinal leech (hirudo medicinalis) in microsurgical practice prompted us to undertake a bacteriological study. aeromonas hydrophila was consistently cultured from 9 leeches that we examined. this organism is pathogenic to man and we consider that there is a significant risk of infection, particularly in those procedures where the vascularity of the tissues is compromised. there are several pharmacological preparations which have similar properties to the secre ...19836831107
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