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travelers' diarrhea among american peace corps volunteers in rural thailand.diarrheal disease was studied prospectively in 35 peace corps volunteers during their first five weeks in rural thailand. twenty (57%) developed the syndrome of travelers' diarrhea. recognized bacterial enteric pathogens were isolated from stools during 47% of 39 episodes of travelers' diarrhea. enterotoxigenic escherichia coli was isolated during 26% and shigella during 13% of the episodes. of the 20 volunteers, 50% had bacteriologic and/or serologic evidence of infection with enterotoxigenic e ...19817019355
enterotoxigenic bacteria in food and water from an ethiopian community.food and water samples from an ethiopian community were screened for the presence of enterotoxin-producing bacteria. using the chinese hamster ovary cell assay, 40 of 213 isolates (18.8%) produced heat-labile (lt) enterotoxin. these lt-producing isolates comprised 33 of 177 (18.6%) strains from 24 of 68 food samples (35.3%) and 7 of 36 (19.4%) isolates of 4 of 17 water samples (23.5%). one lt-producing strain each of salmonella emek and of shigella dysenteriae was found. three pseudomonads, all ...19817016032
[direct immunofluorescence in the diagnosis of aeromonas salmonicida infections in fresh water fishes]. 19817018491
medium for the accumulation of extracellular hemolysin and protease by aeromonas hydrophila.a medium for the accumulation of extracellular hemolysin (300 to 1,600 hemolytic units per ml) and protease (2 to 3 proteolytic units per ml) was developed for an anaerogenic strain of aeromonas hydrophila. in this medium, growth yields were less but levels of accumulated toxin were greater or equivalent when compared with the same responses in brain heart infusion and nutrient broths. the medium was considered to be partially defined since the conditions for maximum observed hemolysin accumulat ...19817026444
[infections caused by aeromonas hydrophila. review of their clinical and bacteriological aspects]. 19817029675
toxin-producing bacteria in infants. lack of an association with sudden infant death syndrome.after finding enterotoxigenic (et) escherichia coli in two consecutive cases of sudden infant death syndrome (sids), we initiated a prospective search for et e coli in sids in manitoba; in addition, we looked for toxigenic clostridium botulinum and c difficile. in a 21-month period, small- and large-bowel contents were obtained in 33 cases of sids, from 32 control subjects, and in ten suspected cases of sids. neither c botulinum nor et e coli was isolated from any of these; c difficile was cultu ...19817032280
extracellular virulence factors of aeromonas hydrophila in fish infections.aeromonas hydrophila nrc 505 produced extracellular substances which were capable of causing pathological effects when injected into trout. proteolytic activity and haemolytic activity of the extracellular products, and the effect on fish, were lost on heating. the extracellular substances from strain g35, a protease-deficient mutant, were significantly more toxic to both rainbow trout (salmo gairdneri) and speckled trout (salvelinus fontinalis) than the analogous preparation from the parental s ...19817032678
taxonomy and description of vibrio fluvialis sp. nov. (synonym group f vibrios, group ef6). 19816971864
aeromonas hydrophila in wild-caught frogs and tadpoles (rana pipiens) in minnesota.frogs and tadpoles were captured at 14 sites in and near minnesota during 1978-79 and nearly all appeared healthy. aeromonas hydrophila was isolated from 94 of 294 (32%) juvenile and adult frogs and from 66 of 104 (63%) tadpoles. of the isolates from frogs and tadpoles respectively, 68% and 47% were from the intestine only, 12% and 32% were from the intestine and the other sites, and 20% and 21% were from extraintestinal sites only. isolations were more frequent from frogs collected in march-jun ...19816972468
aeromonas toxins. 19817048346
role of ph, lactate, and anaerobiosis in controlling the growth of some fermentative gram-negative bacteria on beef.at 5 degrees c four strains of fermentative, gram-negative bacteria (serratia liquefaciens, yersinia enterocolitica, enterobacter cloacae, and aeromonas hydrophila) grew aerobically and anaerobically on adipose tissue removed from beef muscle of low ph (5.4 to 5.6). all four strains also grew aerobically and anaerobically on muscle tissue of high ph (6.0 to 6.3). however, none of the four grew anaerobically on beef muscle of low ph, and the aeromonad strain also failed to grow aerobically on suc ...19817316511
cytotoxicity and suckling mouse reactivity of aeromonas hydrophila isolated from human sources.human isolates of aeromonas hydrophila and plesiomonas shigelloides were tested for their ability to produce cytotoxins and (or) enterotoxins. the incidence of cytotoxin production by a. hydrophila was 81% for isolates from stool and 44% for extraintestinal isolates. chinese hamster ovary (cho) cells were more sensitive to a. hydrophila cytotoxin than either vero or y-1 mouse adrenal tumor cells (y-1). there was no evidence of cytotonic enterotoxin production by any of the isolates tested. cytot ...19817317856
improved method for cholera diagnosis.the use of the oxidase test is proposed to discover sparse colonies of cholera vibrios on a plate of pril nutrient agar seeded directly with the suspected stool sample or after enrichment. this method also enables the detection of other oxidase-positive potential pathogens, such as non-agglutinating vibrios, aeromonas hydrophila, pseudomonas spp., and other nonfermentative organisms.19817229013
salmonella and other pathogenic bacteria.the bacterial origin of waterborne diseases was discovered at the turn of this century. introduction of slow sandfiltration, chlorination, and bacteriological control dates back to the same period. although greater concern is given to-day to chemical pollutants or to viruses, bacteria are still a menace to countries with advanced water treatment. within the last decade outbreaks were reported in europe and the us due to salmonella types, shigella, e. coli, and to vibrio cholerae, generally due t ...19817233156
plasma iron, copper, and zinc in lizard dipsosaurus dorsalis: effects of bacteria injection.the effects of inoculation of desert iguanas (dipsosaurus dorsalis) with heat-killed aeromonas hydrophila on the plasma concentrations of iron, copper, and zinc, and the total iron-binding capacity have been determined. plasma iron concentration and total iron-binding capacity fell significantly in inoculated lizards maintained at temperatures corresponding to those selected during fever. plasma copper concentration did not change in response to inoculation (as determined at 24 h postinjection). ...19817235044
adhesion of vibrios and aeromonads to isolated rabbit brush borders.isolated rabbit brush borders were used to investigate the adhesive properties of clinical and environmental isolates of non-o1 vibrio cholerae and clinical isolates of vibrio parahaemolyticus and aeromonas hydrophila. a minority of the isolates were found to adhere to brush borders. the adhesion of these isolates was affected by a number of factors, including the bacteria:brush border ratio, incubation time, culture medium and growth temperature. adhesion was inhibited by l-fucose but was indep ...19817334329
effect of temperature on growth and activity of aeromonas spp. and mixed bacterial populations in the anacostia river.during the winter months, total bacterial counts in the water column and in the sediment in the anacostia river were two- to eightfold higher than at other times of the year, whereas aeromonas spp. decreased in number of several orders of magnitude. this significant decrease in number in the anacostia river during the cold months of the year can be explained by the low metabolic activity of aeromonas at low temperatures.19817235703
unusual disease conditions in pet and aviary birds.ninety percent ot 100% mortality in budgerigar (melopsittacus undulatus) nestlings from 2 aviaries was attributed to giardiasis. treatment with dimetridazole in drinking water was effective in controlling mortality. aeromonas hydrophila infection incriminated in acute deaths of aviary canaries (serinus canarius) was successfully treated with chlortetracycline. aeromonas hydrophila also was isolated in pure culture from a toucan (ramphastos toco) with acute nephrosis and a cockatiel (nymphicus ho ...19817240000
role of ph in oxidase variability of aeromonas hydrophila.some strains of aeromonas hydrophila may be oxidase negative or only weakly oxidase positive by the kovacs method taken from the surface of a differential medium, such as macconkey agar. six strains of a. hydrophila, two oxidase variable, one oxidase constant, and three weakly oxidase positive on macconkey agar, were studied to determine the cause of oxidase variability. the bacteriostatic dyes in macconkey agar were considered possible inhibitors of the oxidase reaction. the concentration of th ...19817251826
nosocomial aeromonas hydrophila cellulitis and bacteremia in a nonimmunocompromised patient.an elderly man, without heat in his home for two days, presented with cold, painful, swollen feet. second-degree frostbite was present. after having moist packs applied to his feet for five days, the patient became febrile and hypotensive, and wet gangrene necessitated left below-knee and right above-knee amputations. aeromonas hydrophila was isolated from the patient's blood and cultures of the pedal wounds. an etiologic role of the moist pack therapy is postulated.19817256345
[on the ecology of plesiomonas shigelloides (author's transl)].studies on the generation time of p. shigelloides and its relation to incubation temperature have shown that this species in marked contrast to a. hydrophila does not multiply in temperature below 8 degrees c. these findings would explain the seasonal variations in the number of isolates from river-water observed in japan. colony counts of p. shigelloides organisms on ibg-agar from water of the river main sampled during the month of august (water temperature 20 degrees c) have shown numbers of 1 ...19817257657
serogrouping of motile aeromonas species isolated from healthy and moribund fish.a total of 195 strains of motile aeromonas isolated from fish were characterized as aeromonas hydrophila and aeromonas sobria. in view of the frequency of isolation and the importance of motile aeromonas species as fish pathogens, a serological classificaton of these organisms was attempted. antisera were prepared in rabbits against formalinized whole cell suspensions and against boiled cells of 12 different isolates. seventy-six strains could be grouped by tube agglutination with whole cells as ...19817259164
a toxigenic profile of aeromonas hydrophila and aeromonas sobria isolated from fish.forty strains of motile aeromonas were isolated from healthy and diseased fish. these strains were identified as a. hydrophila or a a. sobria. it was found that only strains of a. hydrophila produced a dermonecrotic factor and two zones of hemolysis on blood agar. all the strains of a. sobria tested and 72% of the a. hydrophila were enterotoxigenic. finally, we observed that only a. hydrophila strains could regularly produce hemolysin at 10 degrees c.19817237279
the possibility of differentiating vibrio and aeromonas on the basis of correlation of the nucleotide composition of dna and the peculiarities of the antigenic structure.the nucleotide composition of dna was correlated with the peculiarities of the antigenic structure of vibrios and aeromonades. the authors propose a supplementary test for the differentiation between these microorganisms by the detection of thermolabile "indicator" antigens, most characteristic of cholera vibrios and pathogenic nag vibrios, in immunoelectrophoresis with diagnostic serum to alcoholic preparation of nag-vibrio flagellin.19817252145
aeromonas hydrophila infection in xenopus laevis.aeromonas hydrophila caused severe disease in a group of xenopus laevis within 3 weeks of receipt. the primary clinical signs were marked pallor of the skin, petechiation, lethargy, anorexia, and edema. the duration of the outbreak was approximately 45 days during which time 21 frogs became sick and 18 died, despite tetracycline therapy. pale skin, subcutaneous edema and hemorrhages, ascites, and pale livers were seen at necropsy. aeromonas hydrophila was isolated from skin, and the same organis ...19817265911
r factors in coliform-fecal coliform sewage flora of the prairies and northwest territories of canada.coliform and fecal coliform populations found in the raw sewages and final sewage effluents of the prairie provinces and the northwest territories were examined for antibiotic resistance and the possession of r factors. it was determined that 8.91% of the total coliform and 10.80% of the fecal coliform populations carried r factors. the following numbers of combinations of r determinants were found: 39 in the escherichia coli population, 6 in the citrobacter population, 20 in the enterobacter po ...19817283425
pneumonia and bacteremia due to aeromonas hydrophila.aeromonas hydrophila is a gram-negative bacillus which has been rarely identified as a human pathogen except in immunologically compromised hosts. we have recently treated three patients for severe a hydrophila pneumonia and sepsis. two of these patients were healthy young men who aspirated the organism from contaminated water associated with near drowning. one patient survived severe ards and gram-negative sepsis. a third patient with chronic renal failure acquired a hydrophila pneumonia at hom ...19817273876
purification and disposition of a surface protein associated with virulence of aeromonas salmonicida.virulent strains of aeromonas salmonicida observed by electron microscopy were characterized by an outer layer exhibiting a tetragonal repeat pattern. attenuated strains had a 2.5 x 10(3)- to 5 x 10(3)-fold reduction in virulence and lost the outer layer, autoaggregating properties, and a 49-kilodalton protein (a protein) simultaneously. the a protein is the major protein component of outer membrane fractions of virulent strains. a variety of radiolabeling studies showed that this protein was su ...19817275932
pyrogens fail to produce fever in a cordylid lizard.we investigated the effects on body temperature of the lizard cordylus cataphractus of intracardiac injections of leucocyte pyrogen (lp) synthesized from rabbit blood and of killed aeromonas hydrophila, a gram-negative bacterium reputed to be pathogenic in lizards. lizards were placed in a photothermal gradient that allowed them to select a preferred body temperature following the injections. neither injection of 0.5 ml rabbit lp nor of 4 x 10(9) organisms of a. hydrophila in 0.2 ml sterile sali ...19817282964
[histological and bacteriological examination of uterus from the repeat breeder gilt and sow (author's transl)].the investigation comprised sexual organs from 25 gilts and 97 sows culled because of repeat breeding. among the gilts 24.0 per cent and among the sows 25.8 per cent had endometritis. the following germs were present in uterus from gilts and sows with signs of endometritis: staphylococcus aureus, corynebacterium pyogenes, alpha-hemolytiske streptococcer, escherichia coli, pasteurella sp., aeromonas sp., acinetobacter sp. and citrobacter sp. among gilts and sows with germs present in uterus 40.9 ...19817322875
[etiology of erythrodermatitis of the carp]. 19817324642
loss of virulence during culture of aeromonas salmonicida at high temperature.the effect of growth temperature on the loss of virulence of the fish pathogen aeromonas salmonicida was investigated. three virulent strains were grown in trypticase soy broth at temperatures ranging from 22 to 30 degrees c. growth at a higher-than-optimal temperature (26 to 27 degrees c for the three strains studied) resulted in the selection of spontaneous attenuated derivatives in the initial bacterial population. for example, virulent bacteria represented less than 10% of the population of ...19817287625
[septicemia caused by aeromonas hydrophila]. 19817302303
detection of enterotoxins of aeromonas hydrophila by a suckling-mouse test.the suckling-mouse assay was reliable for detecting enterotoxigenic strains of aeromonas hydrophila when standard conditions for growth and toxin testing were used. enterotoxins were produced by bacteria grown in tryptone soya broth supplemented with yeast extract and aerated by shaking in an environmental incubator or water bath. when culture supernates together with dye were administered intragastrically to mice less than 6 days old, the presence of enterotoxin was assessed on the basis of a s ...19817310844
isolation and characterization of 3-acetamido-3,6-dideoxy-l-glucose from the core oligosaccharides obtained from the aquatic gram-negative bacteria aeromonas hydrophila and vibrio anguillarum. 19817332867
membrane-damaging and cytotoxic effects on human fibroblasts of alpha- and beta-hemolysins from aeromonas hydrophila.the effects of two hemolysins (alpha and beta) from aeromonas hydrophila on human lung fibroblasts were investigated. the toxins differed distinctly in regard to the morphological changes they produced. the alpha-hemolysin caused rounding of the cells. the beta-hemolysin caused a striking vacuolization of the cytoplasm in cells which remained spread out on the growth surface. the toxins also differed as to relative size of the initial lesions they induced in the fibroblast membrane, scored by le ...19817333677
separation and characterization of enterotoxin and two haemolysins from aeromonas hydrophila.aeromonas hydrophila produces two haemolysins, now designated alpha- and beta-haemolysin (formerly aerolysin or cytotoxic protein), and one enterotoxin. these toxins were separated and purified by isoelectric focusing and gel chromatography. alpha- and beta-haemolysin differ with regard to prerequisites for their elaboration, such as selection of a. hydrophila strains and temperature and time for cultivation. the nature of their lytic effects on erythrocytes and toxic effects on tissue culture c ...19817336925
aeromonas hydrophila infection of the skin. 19817212750
microbial flora of the lower genital tract of women in labour in zaria, nigeria.nine genera of microbes isolated from the lower genital tract of 187 women in labour in zaria have been identified. the work was undertaken to establish the nature of microorganisms in the lower genital tract of women in labour as a basis for further study. the isolates in order of prevalence were: candida albicans (20.9%), klebsiella sp (15.0%), escherichia coli (9.1%), streptococcus faecalis (6.4%), haemolytic streptococci (other than streptococcus pyogenes (2.7%), streptococcus viridans (2.1% ...19817007447
evaluation of a test-kit (oxi/ferm system) for identification of vibrios.a total of 258 strains belonging to different species of vibrio isolated from various sources in different parts of the world were subjected to identification by oxi/ferm tube and conventional tests sometimes extending to 250 characters when the conventional tests failed to recognise them. nearly 88 per cent of them could be identified accurately in this system at the species level. the strains designated as mannitol negative vibrios and vibrio species were also identified properly as v. cholera ...19817036597
aeromonas hydrophila bacteraemia in haematological patients. 19817242421
molecular characterization of environmental and nontoxigenic strains of vibrio cholerae.environmental and nontoxigenic strains of vibrio cholerae 0-1 were examined for genes homologous to genes encoding escherichia coli heat-labile enterotoxin (lt). restriction fragments encoding lt a and b subunits were isolated from the recombinant plasmid ewd299 and labeled in vitro with 32p. these probes were then hybridized to deoxyribonucleic acid extracted from strains of v. cholerae and visualized by autoradiography. none of the nontoxigenic strains of v. cholerae 0-1 from louisiana, alabam ...19817251142
aeromonas hydrophila: ecology and toxigenicity of isolates from an estuary. 19817251516
mortality in captive atlantic cod, gadus morhua, associated with fin rot disease.a total of 320 of 621 (52%) captive atlantic cod, gadus morhua, died from fin rot over an 8-year period; mortality reached high proportions in 1979 when 110 of 168 (66%) succumbed. lesions were confined to the fins, skin and dermal musculature and were observed 3-10 days after the fish were placed in laboratory aquaria. erosion of the fins and caudal peduncle, accompanied by petechiae and ulceration also were apparent in the trunk region. most deaths occurred within 2 months after capture. infec ...19817338975
a bacterial disease of perch (perca fluviatilis l.) in an alpine lake: isolation and preliminary study of the causative organism.an epizootic among perch (perca fluviatilis l.) occurred during the summer and fall of 1979 in an alpine lake (lake annecy, haute-savoie) in france. hemorrhagic and ulcerative clinical signs were associated with the height of the mortality. a gram-negative, non-motile, slow-growing bacterium was isolated from skin lesions is diseased fish. aeromonas hydrophila often was present. since the nonmotile bacterium was suspected to be the etiological agent, its characteristics and pathogenicity were de ...19817338972
exotoxins of aeromonas hydrophila.eighty of 103 strains of aeromonas hydrophila cultured at 100 rev./min produced heat-labile enterotoxins detected using the suckling mouse assay. results in intestinal perfusion agreed with the suckling mouse test in all strains tested by both methods. enterotoxic activity correlated with haemolysin and cytotoxin production, but 4% of strains would have been wrongly classified using haemolysin assay in place of the suckling mouse test and 11% misclassified on the basis of cytotoxin assay. there ...19817340773
in vitro susceptibilities of aeromonas hydrophila to 32 antimicrobial agents.minimal inhibitory concentrations of 32 antimicrobial agents for 20 strains fo aeromonas hydrophila were determined by a microdilution method. moxalactam was the most active drug tested. all strains were also susceptible to clinically achievable concentrations of mecillinam, cefamandole, cefuroxime, cefotaxime, aminoglycosides (except streptomycin), tetracycline, chloramphenicol, and trimethoprim-sulfamethoxazole.19817347568
bacterial survival in a dilute environment.bacteria were isolated from lake water, and their ability to remain viable in a dilute, nutrient-deficient environment was tested by a method that permits suspension of test bacteria between two appressed microporous membranes in an aqueous environment. this approach permitted separation of the lake isolates into two categories. members of the tribe klebsielleae were shown to have a prolonged survival rate of 40% or better after 24 h, whereas nonsurvivors were not viable for much longer than 24 ...19816454390
elevation of adenosine 3' 5' cyclic monophosphate level by aeromonas hydrophila enterotoxin. 19816281180
association of aeromonas sobria with human infection.fifteen aeromonas isolates from various human infections and nine isolates from polluted water were identified as either aeromonas hydrophila or aeromonas sobria and examined for cytotoxigenicity, enterotoxigenicity, adherence to epithelial cells, and other virulence-associated factors, including proteases, lipases, elastases, and hemolysins. two groups of organisms (i and ii) were distinguishable based on differences in median lethal doses in mice and cytotoxicity for y-1 adrenal cells. group i ...19816112237
viruses and bacteria in pediatric diarrhea in thailand: a study of multiple antibiotic-resistant enteric pathogens.children with diarrhea admitted to a rehydration ward of a children's hospital in bangkok were investigated to determine the prevalence of enteric pathogens, the extent of children's previous antibiotic therapy, and the frequency of plasmid-mediated antibiotic resistance among infecting bacteria. rotavirus (36%), enterotoxigenic escherichia coli (18%), shigella (9%), salmonella (6%), campylobacter jejuni/coli (4%), and vibrio cholerae (2%) infections were among 105 children with diarrhea. antibi ...19816275727
aeromonas bacteraemia in a burn patient. 19816117752
clustering of aeromonas hydrophila septicaemia. 19816118660
nonselectivity of rimler-shotts medium for aeromonas hydrophila in estuarine environments.thirty presumptive aeromonas hydrophila isolates were collected from estuarine sources using rimler-shotts media. many of the isolates, especially the strains from a low-salinity site, were also identified as a. hydrophila using the api 20e system and two differential techniques devised for the identification of a. hydrophila. all of the isolates, however, had deoxyribonucleic acid base compositions with guanine-plus-cytosine ratios between 40 and 49 mol%, which excludes these strains from the g ...198116345850
effects of pharmaceutical wastes on microbial populations in surface waters at the puerto rico dump site in the atlantic ocean.a series of cruises during 1979 and 1980 to the pharmaceutical dump site located 64 km north of arecibo, puerto rico, in the atlantic ocean, was carried out to evaluate effects of wastes on the ecology of the microflora of surface waters of the dump site. in addition to bacteriological monitoring of the waste plume created by the release of wastes from the disposal barge, stations along a series of transects, extending north from coastal waters through and beyond the dump site, were sampled. lar ...198116345752
external microflora of a marine wood-boring isopod.bacteria associated with the marine wood-boring isopod limnoria lignorum were enumerated by acridine orange epifluorescence microscopy and by plate counts on several media; the plate-viable bacteria were isolated and identified. similar procedures were followed to enumerate and identify bacteria associated with the wood substrate from which the isopods were collected and with the surrounding water from the isopod habitat. approximately 1.4 x 10 bacterial cells were associated with each individua ...198116345871
growth of aeromonas hydrophila at low concentrations of substrates added to tap water.the ability of an aeromonas hydrophila isolate obtained from filtered river water to grow at low substrate concentrations was studied in batch experiments with tap water supplied with low concentrations of substrates. growth was assessed by colony count determinations. the isolate only multiplied in the used tap water (2 to 3 mg of dissolved organic carbon per liter) after the addition of a small amount of an assimilable carbon compound. d-glucose especially caused growth of the organism even at ...198016345582
distribution and characterization of kepone-resistant bacteria in the aquatic environment.effects of the chlorinated insecticide kepone on the ecology of chesapeake bay and james river bacteria were studied. kepone-resistant bacteria present in a given environment were found to reflect the degree of fecal and/or high organic pollution of the sampling sites, based on total numbers and generic composition of the populations of kepone-resistant bacteria. the presence of kepone-resistant bacteria was found to be correlated (alpha = 0.01) with total coliforms, fecal coliforms, and total a ...19806155825
hemagglutination properties and adherence ability of aeromonas hydrophila.human diarrheal isolates and enterotoxigenic strains of aeromonas hydrophila are strong hemagglutinators with human blood cells. sugar inhibition studies and yeast coagglutination tests with 11 selected strains revealed six different hemagglutination mechanisms for this species. these were characterized by inhibition by l-fucose, inhibition by d-galactose, inhibition by d-mannose, and two distinguishable mechanisms which were inhibited by either l-fucose or d-mannose, one being pilus mediated. i ...19806103874
a study on the possible association of copper pollution with vibriosis in eel.in this study, eel from a locality where the bacterium vibrio anguillarim was common were transferred to copper-contaminated freshwater and brackish water (10%) under laboratory conditions. in one experiment (experiment i) 9 out of 20 eel died and bacteria (in six cases v. anguillarum and in two cases aeromonas sp.) were isolated from the kidneys, and one was found to be sterile. in this experiment copper did not seem to be an initating factor in developing bacterial infection. in two other expe ...19807360621
ocular aeromonas hydrophila.aeromonas hydrophila was isolated from the eye swabs of four patients. two were thought to have definite clinical infection; one had chronic conjunctivitis complicating the wearing of contact lenses, and the other had acute conjunctivitis associated with ectropion and lacrimal duct stenosis. two patients had no clinical evidence of infection. both of them had swabs taken at the time of surgery. one had a cataract extraction and the other had a lensectomy and vitrectomy for a penetrating injury. ...19807369306
fatty acid patterns in the classification of some representatives of the families enterobacteriaceae and vibrionaceae.twenty-three strains representing the families enterobacteriaceae and vibrionaceae were analysed for fatty acid composition of whole cells by means of glass capillary column gas chromatography. among the several alternatives tested, cluster analysis based on data normalized to hexadecanoate and logarithmically transformed provided good separations of species, genera and families. strains from the genera salmonella, escherichia, proteus, enterobacter, klebsiella, vibrio and aeromonas were studied ...19807365455
plesiomonas (aeromonas) shigelloides septicemia and meningitis in a neonate.serious plesiomonas (aeromonas) shigelloides infections have rarely been reported, and have probably been missed because this organism is very similar to the enterobacteriaceae in associated clinical disease, and in properties investigated in the diagnostic laboratory. a case of overwhelming neonatal meningitis and sepsis is discussed, and the use of the simple indophenol oxidase test on laboratory isolates of gram-negative rods is urged to distinguish this organism and its close relatives from ...19807361158
[allomonas--a new group of microorganisms of the vibrionaceae family. i. research methods and preliminary results of differention from aeromonads and vibrios].the comparative study of 95 strains of aeromonas, plesiomonas and various vibrio species by 49 characteristics (disregarding those common to all representatives of the vibrionaceae family) allowed to distinguish a separate group of microorganisms differing from aeromonas by 17 characteristics, from plesiomonas by 13 characteristics and from vibrio by 7 characteristics. the new microogranism was provisionally named allomonas. the taxonomic study of the new microorganism continues.19807376760
optional production and utilization of polysaccharide by aeromonas hydrophila.glucans from the fish pathogen aeromonas hydrophila have been extracted and purified by a method utilizing phenol/water followed by sodium deoxycholate rather than the traditional sodium hydroxide extraction. presence of substantial amounts of these glucans was shown to be dependant on whether or not the substrate contained dextrose, a point which had import because of the low carbohydrate environment in which this species must survive and multiply. these glucans, produced in the log phase, were ...19807387332
metabolism of coho salmon ig. catabolic rate of coho salmon tetrameric ig in serum. 19807393235
biochemical characteristics and virulence of environmental group f bacteria isolated in the united states.bacteria phenotypically resembling aeromonas hydrophila, but requiring nacl for growth, have been isolated form the new york bight. the bacteria proved to be identical to group f organisms isolated from cases of human diarrhea in indonesia and bangladesh. anaerogenic strains initiated responses in y-1 tissue culture and rabbit ileal loop, consistent with those associated with cytotoxin- and enterotoxin-producing aeromonas spp. strains. separation on the basis of production of gas from glucose by ...19807425623
[genome characteristics of a new group of microorganisms in the vibrionaceae family].a new group of microorganisms isolated from river and sewage water was studied by the method of molecular dna-dna hybridization and included into the family vibrionaceae on account of their phenotypical properties. the genomes of the microorganisms belonging to this group proved to be highly similar (41-92% of homologies), which suggested that they were related on the genus level. the degree of similarity between the nucleotide sequences in the dna of the bacteria under study and the dna of othe ...19807415669
hand and forearm infections caused by aeromonas hydrophila.this is a report of two cases of hand and forearm infections caused by aeromonas hydrophila. this organism is resistant to penicillin and most cephalosporins and is found in most freshwater environments. it is recommended that broad-spectrum coverage such as a combination of cephalosporin and aminoglycoside antibiotic be used.19807430608
fever and antipyresis in the crayfish cambarus bartoni.1. normothermic cambarus bartoni crayfish thermoregulating behaviourally in an electronic shuttlebox prefer a mean temperature of 22.1 degrees c +/- 0.5 s.e. of mean. 2. injection of 1% saline, or addition of paracetamol to the ambient water, caused a statistically insignificant decrease in preferred temperature of 0.4 degrees c. 3. injection of killed aeromonas hydrophila bacteria into the abdomen caused a significant mean increase in preferred temperature of 1.8 degrees c (defined as a behavio ...19807431243
an eye infection in laboratory lizards associated with an aeromonas sp.laboratory-maintained lizards (family lacertidae) kept for studies on thermal biology developed a superficial eye infection associated with aeromonas liquefaciens. the origin of the infection could not be traced and attempts at therapy were generally unrewarding.19807431825
enterotoxigenicity of aeromonas strains in suckling mice.the enterotoxigenicity of 170 aeromonas strains isolated from different sources (food poisoning, random food sampling, water, faeces) was examined by the suckling mouse test. the strains were grown on syncaye culture medium covered with sterilized membrane for kiil-kidney. the culture supernatants were inoculated orally. ileal loop dilatation was compared to that produced by the international standard enterotoxic escherichia coli b7a (o148 : h28) and b2c (o6 : h16) strains. of the 87 aeromonas h ...19807415872
fulminant wound infection with aeromonas hydrophila.aeromonas hydrophila was isolated from a water-soaked traumatic wound of a previously healthy 16-year-old boy. a progressive necrotizing infection developed despite administration of antibiotics. aeromonas should be considered in wound infections which are water-related.19807444534
[differentiation of bacteria of the genus aeromonas from other representatives of the vibrionaceae family on the basis of their dna].by comparing the data on the nucleotide composition of dna, and the phenotypic characteristics, most of the representatives of the genus aeromonas were clearly differentiated from nag vibrios isolated in the process of sanitary control of the environment. at the same time some microorganisms with the aeromonas phenotype, but having a different dna structure were detected. the use of the method of molecular dna hybridization indicated that these bacteria were the taxons analogous to the genus aer ...19807445866
[allomonads, a new group of microorganisms of the vibrionaceae family. iii. taxonomic analysis of the similarities between allomonads and other genera of the family].24 strains of gram-negative bacilli with the polar location of flagellae, oxidase positive, releasing no gas in the process of glucose fermentation in aerobic and anaerobic conditions in the high-leifson test, belonging to the family vibrionaceae, were isolated from the feces of healthy persons, polluted water and sewage. analysis by the method of numerical taxonomy revealed that these strains were essentially different from all other members of this family, and 19 of them formed a sharply defin ...19807445867
false-negative oxidase reaction as a result of medium acidification.acidification of the culture medium may lead to a false-negative result of the oxidase reaction. hence, if the medium contained a carbon source or another component that is convertible to acid, a negative oxidase reaction should be considered inconclusive. in order to eliminate false-negative reactions, a reagent freshly adjusted to ph 5-7 might be used; this reagent should be checked on known oxidase-negative strains. however, we recommend that the oxidase test be performed with the normal reag ...19807447434
comparison of nine brands of membrane filter and the most-probable-number methods for total coliform enumeration in sewage-contaminated drinking water.nine different brands of membrane filter were compared in the membrane filtration (mf) method, and those with the highest yields were compared against the most-probable-number (mpn) multiple-tube method for total coliform enumeration in simulated sewage-contaminated tap water. the water was chlorinated for 30 min to subject the organisms to stresses similar to those encountered during treatment and distribution of drinking water. significant differences were observed among membranes in four of t ...19807469407
lipopolysaccharides of group f, a new group of vibrios. 19807470107
identification and characterization of outer membrane fragments released by aeromonas sp.aeromonas hydrophila and aeromonas salmonicida were shown to release "blebs" or fragments averaging 20-30 nm in diameter. the protein composition of the fragments was very similar to that of the corresponding outer membrane by sodium dodecyl sulphate--polyacrylamide gel electrophoresis and the fragments were shown to contain phospholipid and lipopolysaccharide. the results therefore indicate that the blebs are derived from the outer membrane of these organisms. fragments isolated directly by dif ...19807459670
[aeromonas hydrophila as a normal intestinal bacterium of the vampire bat (desmodus rotundus)]. 19807053178
an epidemic of extrinsic allergic alveolitis caused by tap water. 19806988107
[quantitative study of the faecal flora in adult and baby artifically fed from birth (author's transl)].the faecal flora of 33 adults and 14 babies (artificially fed from birth) were determined. the faecal flora were most different. the primary anaerobic flora (prevalent bacteroïdes and bifidobacterium) in adults was 99 p. cent from total flora; it was in babies (prevalent bifidobacterium and bacteroïdes) 50 at 60 p. cent from total flora. the primary aerobic flora (e. coli and enterococcus) was 10 to 20 times more numerous in babies than in adults. therefore the secondary flora was very distinct. ...19806988778
enterotoxicity of group f vibrios.recently a group of organisms resembling aeromonas hydrophila has been isolated from human diarrheal cases from marine and estuarine environment. these organisms are tentatively designated as group "f" vibrios. live cells of 23 strains isolated from different sources were tested for their enterotoxicity in the adult rabbit ileal loop model. all but two caused fluid accumulation comparable to that obtained with vibrio cholerae 569b. an inoculum of 10(4) viable bacteria could give rise to gut loop ...19807218576
cultural and biochemical characteristics of clinical isolates of aeromonas hydrophila. 19807269636
aeromonas hydrophila as the etiologic agent in severe gastroenteritis: report of a case.aeromonas hydrophila was isolated from stool of an 82-year-old female with severe gastroenteritis. the stool was foamy and varied in color from green to yellow. the isolated organism was susceptible to most aminoglycosides. the tetracycline therapy cleared the organism from the stool and the patient's condition improved. the biochemical characteristics of the organism were similar to coliform bacilli: the oxidase test was necessary to differentiate aeromonas sp. from coliforms. the patient did n ...19807361816
persistence of the virulence of aeromonas salmonicida strains kept in river sediments.the persistence of pathogenicity in two strains of aeromonas salmonicida maintained in river sediments was studied at different times. cultures were kept about nine months, and the virulence was compared to its initial value exhibited by the same strain, subcultivated in rainbow trout fingerlings. trials involved intramuscular injection of comparable doses of bacteria to batches of 30 fish held at 15 degrees c, and recording of subsequent mortalities. no difference was found during the first six ...19807337394
enumeration and characterization of standard plate count bacteria in chlorinated and raw water supplies.nearly 700 standard plate count (spc) bacteria were isolated from drinking water and untreated surface water and identified according to a scheme developed to permit the rapid, simple classification of microorganisms to genus, species, or group. actinomycetes and aeromonas species were the two most common groups of spc bacteria in chlorinated distribution water. aeromonas spp. and enterobacter agglomerans were the two most common groups of spc bacteria in raw water. identification of bacterial p ...19807447444
[biochemical properties and differentiation of vibrionaceae isolated from water and water-oil emulsion]. 19807311653
aeromonas hydrophila infection of a puncture wound.aeromonas hydrophila is a gram-negative organism generally considered a pathogen of low virulence, rarely reported as causing infection in man. recently it has been recognized as causing infection in wounds. this article reports the case of a patient who suffered a puncture wound while standing in a fresh water lake. he subsequently developed a rapidly progressing cellulitis requiring hospitalization. this organism was cultured from the wound and responded dramatically to appropriate antimicrobi ...19807425408
r-factor carriage in a group f vibrio isolated from bangladesh.two group f vibrio organisms have been identified among a collection of vibrio strains isolated from the aquatic environment in bangladesh. neither group f strain produced a cholera-like enterotoxin. one of the isolates, bv12, contained an r plasmid conferring resistance to streptomycin and chloramphenicol.19807425610
[enterobacteria of bats (chiroptera) (author's transl)].the aerobic gram-negative faecal flora of 38 bats consisting of 10 species and genera respectively, of microchiroptera, and of 4 species and genera respectively, of megachiroptera was studied (table 1 and 3). there were no specific differences between insectivora and frugivora: e. coli 15-24%, citrobacter 8-10%, enterobacter-klebsiella-group 40-43% and proteus-group 28-30% (table 2). the overwhelming majority of the isolated bacteria were lactose-positive (table 3), corresponding to the membersh ...19807424248
dysentery-like syndrome associated with aeromonas hydrophila. 19807427550
isolation of drug-resistant aeromonas hydrophila from aquatic environments.antibiotic-resistant strains of aeromonas hydrophila have been isolated from the natural environment in the chesapeake bay and areas surrounding dacca and the matlab region of bangladesh. the bangladesh strains carried resistance to chloramphenicol, streptomycin, and tetracycline, and 57% of them had a multiple streptomycin-tetracycline resistance phenotype correlated with the presence of a large plasmid. the chesapeake bay strains were resistant to polymyxin b ane tetracycline, but showed neith ...19807425607
evaluation of a test kit (oxi/ferm system) for identification of oxidase positive gram negative neoenteropathogenic bacteria.during recent years a number of oxidase positive gram negative bacteria have been implicated as causative agents of outbreaks of acute diarrhoeal disease and food-poisoning in many parts of the world. as taxonomic positions of many of them are not yet well-defined, their identification is often difficult and time consuming. this oxi/ferm tube system was evaluated in relation to the conventional battery of tests with 128 organisms isolated from different sources in various parts of the world. abo ...19807223128
[study of some strains of aeromonas hydrophila of human origin].a detailed description is given of the properties of 14 aeromonas hydrophila strains: morphological, growth in cultures, biochemical and sensitivity to antibiotics. three of the strains were agglutinated by shigella schmitzi antiserum. most of the strains were isolated from 8 cases of enteritis, one from a fatal case of meningitis, and three strains from the meconium of a neonate and, subsequently, from necropsy specimens. except for the strain isolated from the meninges, the others were obtaine ...19807423091
isolation, enumeration, and characterization of aeromonas from polluted waters encountered in diving operations.counts of total viable, aerobic, heterotrophic bacteria, indicator organisms, and aeromonas spp. were made at a diver training site on the anacostia river in washington, d.c. the numbers of aeromonas cells in anacostia river sediment and water increased during periods of elevated water temperature, to maxima of 4 x 10(5) cells per g of sediment and 300 cells per ml of water. correspondingly, aeromonas counts dropped 2 to 4 logs as the water temperature decreased to 0 to 0.5 degrees c. cultures t ...19807396482
relationships between selected strains of typical and atypical aeromonas salmonicida, aeromonas hydrophila, and haemophilus piscium.serological analyses of typical and atypical aeromonas salmonicida divided the representative strains into two distinguishable groups based on their antigenic composition. however, the serological groupings do not coincide with divisions constructed on the basis of physiological reactions. all of the a. salmonicida strains and the two a. hydrophila strains examined share at least one antigen and are sensitive to a. salmonicida bacteriophage 40 rr2.8t. these data reinforce the reasons for placing ...19807397604
aeromonas hydrophila: analysis of 11 cases.a retrospective analysis of 11 cases in which aeromonas hydrophila was isolated indicated that the organsim caused local infection in 7 cases and asymptomatic colonization in 4. there were no cases of septicemia and none of the patients were known to have a malignant disease or immunosuppression. there were no deaths, although three of the patients required amputation of limbs because of myonecrosis. chloramphenicol and aminoglycosides appeared to be appropriate therapeutic agents.19807388723
salmonella, arizona, shigella and aeromonas isolated from the snail achatina achatina in nigeria.the salmonella, arizona, shigella and aeromonas contents of 39 snails (achatina achatina) were investigated. serotyping of the salmonella isolates revealed the presence of s. manhattan, s. ndolo, s. reading, s. uppsala, and s. typhimurium. six of 18 shigella isolates were identified as sh. sonnei while all the aeromonas proved to be a. hydrophila. fifty eight percent of these a. hydrophila isolates were enterotoxigenic, and their toxin was shown to be heat labile. all the a. hydrophila strains w ...19807235689
aeromonas hydrophila in rainbow trout: relation between virulence and surface characteristics.motile aeromonas isolated from fish were studied for their virulence in fish in relation to some surface characteristics. the results showed that only the most virulent strains of a. hydrophila used in this study shared a common o antigen, did not agglutinate in acriflavine, settled down after boiling, and were resistant to the bactericidal action of fresh normal mammalian serum. the least virulent strains could not be grouped into this o antigenic group, they did not settle after boiling, and w ...19807237273
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