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cloning of enterotoxin gene from aeromonas hydrophila provides conclusive evidence of production of a cytotonic enterotoxin.culture filtrates of two aeromonas hydrophila strains which were isolated from patients with diarrhea and assumed to be causative agents of the infections were shown to contain enterotoxic, cytotoxic, and hemolytic activities. modest heat treatment of the filtrates inactivated the cytotoxic and cytolytic activities, but not the enterotoxic activity. the construction of cosmid gene banks in escherichia coli of dna from both a. hydrophila strains demonstrated that the determinants of the three act ...19846500697
aeromonas hydrophila colitis in a child.a 5-year-old girl presented with chronic bloody diarrhea. evaluation including sigmoidoscopy, rectal biopsy, and barium enema was consistent with the diagnosis of ulcerative colitis. culture of the stool grew aeromonas hydrophila. a. hydrophila colitis may be more common than presently realized.19846502383
media for isolation of aeromonas spp. from faeces.five solid media were evaluated for isolation of aeromonas spp. from faeces: desoxycholate citrate agar (dca), macconkey's agar (mac), xylose-desoxycholate-citrate agar (xdca), rogol's medium (rog), which contained ampicillin 20 mg/l and p-nitrophenyl-glycerine 25 mg/l as inhibitors, and blood agar (ba) with ampicillin 10 mg/l. false negative oxidase tests limited the usefulness of dca and mac and, although the use of xdca avoided the problem of fermentation of lactose, some aeromonas spp. faile ...19846502678
production of cholera-like enterotoxin by aeromonas hydrophila.a total of 249 strains of mesophilic aeromonas including 179 a. hydrophila and 70 a. caviae were tested for production of cholera-like enterotoxin by reversed passive latex agglutination (rpla) assay. a cholera-like enterotoxin neutralized with cholera antitoxin was demonstrated in the culture filtrates from eight (4.5%) of the 179 a. hydrophila strains, while none of a. caviae strains revealed the enterotoxin production in the test. production of the cholera-like enterotoxin in the eight strain ...19846503026
isolation of enterotoxigenic, hemolytic, and antibiotic-resistant aeromonas hydrophila strains from infected fish in bangladesh.strains of aeromonas hydrophila isolated from skin infections of common freshwater fish in bangladesh were tested for enterotoxin production, hemolysin production, and any correlation between these two activities. we also tested the resistance patterns of a. hydrophila to different drugs, especially in relation to ampicillin. the a. hydrophila strains produced an enterotoxin that was related to their beta-hemolytic activities. production of beta-hemolysin may thus be an indicator of enterotoxici ...19846508295
plasmid coding for transferable drug resistance in bacteria isolated from cultured rainbow trout.the occurrence of drug resistance and plasmid-mediated transferability was investigated in 170 strains belonging to eight bacterial groups isolated from cultured rainbow trout. it was found that 87.6% of the strains were resistant to at least one drug, with the highest percentages of resistance being detected for ampicillin (54.7%), sulfadiazine (46.5%), nitrofurantoin (38.2%), and chloramphenicol (37.0%). six enterobacteria, two vibrio, and one aeromonas isolate transferred resistance factors t ...19846508296
aeromonas septicemia with shock in children with thalassemias. 19846512449
value of blood agar for primary plating and clinical implication of simultaneous isolation of aeromonas hydrophila and aeromonas caviae from a patient with gastroenteritis.the simultaneous recovery of aeromonas hydrophila and aeromonas caviae from the stool of a 49-year-old woman with watery diarrhea was facilitated through the use of a blood agar medium which detected the hemolytic capability of a. hydrophila. in vitro phenotypic tests support the conclusion that only the a. hydrophila isolate was clinically significant.19846520231
surface layer virulence a-proteins from aeromonas salmonicida strains.superficial surface layer proteins (a-proteins) were present on diverse isolates of aeromonas salmonicida which differed both physiologically and in pathogenesis. three of these proteins were purified directly from the surface of whole cells or from outer membrane preparations. these a-proteins were unusually hydrophobic (45-47%) and of similar but not identical molecular mass (49, 50, and 51 kdaltons). they were nearly identical in amino acid composition and were highly conserved, but not ident ...19846525563
phylogenetic relationships among vibrio anguillarum plasmids.results of restriction endonuclease analysis and southern blot hybridization suggest that the r-plasmids from vibrio anguillarum strains isolated in japan can be divided into at least four groups of homology depending on the time of their isolation and geographical source. molecular cloning experiments allowed identification of specific restriction endonuclease fragments carrying the genes for either cmr or tcr as the common sequences between some of these groups of r-plasmids. the cmr region fr ...19846528000
aeromonas hydrophilia tenosynovitis in an immunocompromised host. 19846536592
o-serogrouping scheme for mesophilic aeromonas strains.the o-antigens of 307 strains of mesophilic aeromonas including 227 a. hydrophila and 80 a. caviae were studied and 44 o-serogroups defined among them. the presence of heat-labile masked antigen, which inhibits o-agglutination, was observed in some strains. as all the o-antisera prepared with these mesophilic aeromonas strains contained some r-antibody, all diagnostic o-antisera must be absorbed with r-organisms before use. some of the o-antigens were found to be identical or closely related to ...19846536785
[aeromonas hydrophila: epidemiological study in the area around rome]. 19846536924
brief report: bacteriological sampling of telephones and other hospital staff hand-contact objects.in an acute-care general hospital, 114 telephones, intercoms, dictaphones, and bedpan flusher handles were sampled in patient-care areas for type of bacterial contamination. nine of these (7%) demonstrated potentially pathogenic bacteria including klebsiella, enterobacter, pseudomonas and aeromonas. inanimate, environmental, staff hand-contact objects were only lightly contaminated, did not represent a significant reservoir of gram-negative organisms, and therefore, would be unlikely to be a veh ...19846568216
aeromonas hydrophila: a treatable cause of diarrhea. 19846607454
hemagglutination patterns of aeromonas spp. in relation to biotype and source.aeromonas spp. show patterns of hemagglutination with human group o cells in the presence of fucose, galactose, and mannose. these patterns are related to biotype as well as to the source of isolates. there was good correlation between hemagglutination pattern and the presence of diarrhea among strains isolated in western australia, which was the only source with adequate data for classification of children with an without diarrhea. most of the environmental and other nonfecal isolates produced ...19846690466
biotyping of aeromonas isolates as a correlate to delineating a species-associated disease spectrum.a group of 147 aeromonas isolates from diverse clinical and environmental sources was subjected to the biotyping scheme of popoff and veron. of the 147 isolates biotyped, 137 (93%) could be identified, with aeromonas hydrophila predominating (48%) and equal percentages (25 to 27%) of the other two species (aeromonas sobria and aeromonas caviae). a number of additional biochemical properties were found to be significantly associated with one or more of these three species. these included lysine d ...19846690467
bacterial interference with coliform colony sheen production on membrane filters.the membrane filter (mf) method for detection and enumeration of coliform bacteria in drinking water requires that the coliforms both grow and produce a green metallic sheen when the filter is incubated on modified endo medium at 35 degrees c for 22 h. large numbers of noncoliform bacteria, which are enumerated by the standard plate count (spc) technique, can interfere with the detection of coliforms on mf. this paper presents quantitative evidence from laboratory experiments on the interference ...19846696423
enterotoxin production in relation to taxonomic grouping and source of isolation of aeromonas species.a total of 19 of 20 (95%) strains of aeromonas hydrophila biovar hydrophila and 16 of 17 (94%) strains of aeromonas sobria isolated from a variety of clinical and environmental sources were found to be enterotoxin positive. only 2 of 18 (11%) a. hydrophila biovar anaerogenes and 2 of 13 (15%) unidentified aeromonas strains from a similar variety of sources produced enterotoxin. no association was apparent between the source of isolation, in particular diarrheal stools, and enterotoxigenicity; 41 ...19846699147
[infections caused by aeromonas hydrophila]. 19846700763
observations of bacterial diseases of captive snakes in finland.eighteen captive, nonpoisonous snakes which suffered from bacterial infections were studied. the most usual bacteria found were gram-negative rods; especially pseudomonas aeruginosa, aeromonas hydrophila and proteus spp. the most common disorders caused by them were stomatitis, septicaemia, bronchopneumonia and abscesses. the sensitivities of ten isolated bacteria to antimicrobial drugs were tested. neomycin and gentamicin seemed to be effective, especially against p. aeruginosa.19846728671
aeromonas septicemia in infants and children.our experience at the ramathibodi hospital with 20 infants and children who had aeromonas septicemia is reviewed. their ages were from 1 day to 14 years. eighteen patients had underlying diseases: leukemia, 5; aplastic anemia, 4; cirrhosis, 2; thalassemia/hemoglobinopathy, 3; renal failure, 1; ileal perforation, 1; marasmus, 1; and cavernous hemangioma with thrombocytopenia, 1. blood cultures yielded aeromonas hydrophila in all patients, and four patients had polymicrobial bacteremia. fifteen ep ...19846728702
aeromonas hydrophila isolated from polyarthritis in a calf. 19846732673
aeromonas hydrophila in livestock: incidence, biochemical characteristics and antibiotic susceptibility.faecal samples from 110 horses, 115 pigs, 111 sheep and 123 cows were examined for the presence of aeromonas hydrophila, which was also sought in the available drinking water. the overall faecal rate was 11.8%, but significantly more bovine than other samples were found to be positive. there was significant association between the isolation of a. hydrophila from all animal faeces and its presence in drinking water, but this was not found when individual animal groups were analysed separately. an ...19846736644
septicemia due to aeromonas hydrophila. 19846736824
aeromonas hydrophila gastroenteritis in tuscany children. 19846739332
biotyping and virulence factors in clinical and environmental isolates of aeromonas species.biochemical characteristics and virulence factors were compared in 147 aeromonas spp. isolated from patients with diarrhea and in 94 strains isolated from metropolitan water supplies in the same area during the same period. fermentation of arabinose occurred with 58.5% of the environmental strains and 15% of the clinical isolates; 39.4% of the strains from water and 6.8% of the fecal isolates fermented salicin. the frequency of esculin hydrolysis was the same in both groups. ninety-one percent o ...19846742829
severe cellulitis due to aeromonas hydrophilia following immersion injury. 19846745473
purification and properties of amino acid racemase from aeromonas punctata subsp. caviae.an amino acid racemase, which occurs in the cytoplasmic fraction of aeromonas punctata subsp. caviae, has been purified to homogeneity by the criteria of electrophoresis and ultracentrifugation. the enzyme has a molecular weight of about 80,000 and consists of two subunits identical in molecular weight (about 40,000). the enzyme contains 2 mol of pyridoxal 5'-phosphate per mol of enzyme, and exhibits absorption maxima at 280 nm and 420 nm. the holoenzyme is resolved by dialysis against hydroxyla ...19846427786
vaccination of fish: a practical view.commercial vaccines giving protection against three important bacterial fish diseases (enteric redmouth, furunculosis and vibriosis) became available in the united kingdom in 1983. the degree of protection obtained and the cost effectiveness vary according to the method of presentation (injection, dipping or spraying and oral dosing). these methods and the optimum time for vaccination are described.19846431685
aeromonas spp in travellers' diarrhoea. 19846434027
aeromonas spp in diarrhoea. 19846437556
comparative in vitro activities of apalcillin and piperacillin against gram-negative bacilli.the susceptibilities of 317 gram-negative bacilli to apalcillin and piperacillin were determined by standardized microdilution and disk diffusion tests. the respective percentages of strains susceptible to less than or equal to 64 micrograms of apalcillin and piperacillin per ml were as follows: members of the family enterobacteriaceae, 90 and 88%; randomly selected pseudomonas aeruginosa, 100 and 100%; multi-drug-resistant p. aeruginosa, 100 and 75%; and other nonfermenters and aeromonas hydrop ...19846440476
an unusual source of hospital wound infection. 19846440623
characterization of plasmids in bacterial fish pathogen.plasmid profiles of representative fish pathogens, aeromonas salmonicida, aeromonas hydrophila, vibrio anguillarum, pasteurella piscicida, yersinia ruckeri, edwardsiella tarda, and renibacterium salmoninarum, were determined by agarose gel electrophoresis with four different plasmid detection methods. a combination of two methods was required to detect the plasmids present in these strains and to calculate precisely the molecular weights of the plasmids. of 38 strains, 28 harbored one or more pl ...19836822413
evaluation of differential and selective media for isolation of aeromonas and plesiomonas spp. from human feces.we studied nine solid and two liquid media for their suitability to select aeromonas and plesiomonas spp. from human stools, using artificially contaminated samples as well as 254 samples from outpatients with and without diarrhea. media with optimal sensitivity and specificity for aeromonas spp. were alkaline peptone-water, trypticase soy broth with ampicillin, inositol-brilliant green-bile salts agar, dextrin-fuchsin-sulfite agar, xylose-sodium desoxycholate-citrate agar, and pril-xylose-ampic ...19836826700
neonatal septicemia and meningitis due to plesiomonas shigelloides. 19836828345
bacteria in enteric lesions of cattle.thirty-nine species of bacteria were isolated from or demonstrated in the abomasal and small and large intestinal mucosa of 23 adult cattle and 41 calves and identified. the bacteria isolated were related to the gross and microscopical lesions. campylobacters, clostridium perfringens type a, c sordellii, actinobacillus lignieresii, fusobacterium necrophorum, mycobacterium paratuberculosis, aeromonas hydrophila and escherichia coli were all associated with specific lesions. the relationship of ot ...19836829137
the medicinal leech and its use in plastic surgery: a possible cause for infection.recent interest in the use of the medicinal leech (hirudo medicinalis) in microsurgical practice prompted us to undertake a bacteriological study. aeromonas hydrophila was consistently cultured from 9 leeches that we examined. this organism is pathogenic to man and we consider that there is a significant risk of infection, particularly in those procedures where the vascularity of the tissues is compromised. there are several pharmacological preparations which have similar properties to the secre ...19836831107
in vitro activity of new cephalosporins against multi-resistant gram-negative bacteria.the in vitro sensitivities of 47 multi-resistant gram-negative bacilli against six third generation cephalosporins were investigated. overall, moxalactam was the most active compound and cefoperazone the least active. ceftizoxime and ceftazidime were very active against enterobacteriaceae. ceftriaxone and ceftizoxime were active against escherichia coli and klebsiella spp., but were inactive against pseudomonas spp.; achromobacter sp. was resistant to all drugs, while citrobacter freundii was se ...19836832840
intracellular accumulation of extracellular proteins by pleiotropic export mutants of aeromonas hydrophila.pleiotropic export mutants of aeromonas hydrophila were obtained which are unable to release protease, hemolysin, and glycerophospholipid:cholesterol acyltransferase. the synthesis of the proteins was not impaired; they were accumulated in active forms inside the mutant cells. the hemolysin could be isolated from cell contents by immunoprecipitation in a form with the same apparent molecular weight as the wild-type extracellular product, as determined by sodium dodecyl sulfate-polyacrylamide gel ...19836833182
alligator bites and related infections. 19836833985
[allomonads--a new group of microorganisms of the family vibrionaceae. v. taxonomic position of the allomonads based on a study of their dna].the dna of allomonas, a new group of microorganisms, has been studied to determine the taxonomic status of this group. in the nucleotide composition of the dna of a typical representative of this group 57.4 mol % g + c have been revealed, which permits to sharply differentiate allomonas from vibrios and plesiomonas. a relatively low level of similarity between the dna of allomonas and that of other representatives of the family vibrionaceae has been determined by the method of molecular dna hybr ...19836837193
es complexes of aeromonas aminopeptidase: direct observation by stopped-flow fluorescence.intermediates of aeromonas aminopeptidase are monitored through fluorescence generated by radiationless energy transfer (ret) between enzyme tryptophans and the dansyl group of the bound substrate. upon binding of the substrate enzyme tryptophan fluorescence is quenched and substrate dansyl fluorescence enhanced. these processes are reversed upon hydrolysis of the leu-ala bond and release of ala-ded from the enzyme. stopped-flow ret kinetic analysis yields values of kcat = 36 sec-1 and km = 3.7 ...19836838596
aeromonas hydrophila typing scheme based on patterns of agglutination with erythrocytes and yeast cells.an agglutination typing scheme has been developed for strains of aeromonas hydrophila. primary agglutination typing is based on testing agar-grown a. hydrophila cells with human, horse, rat, and guinea pig erythrocytes and saccharomyces cerevisiae cells. further subdivision of primary groups is based firstly on whether yeast cell agglutination is inhibited by a d-mannose polymer, yeast mannan, and secondly on patterns of inhibition of hemagglutination by yeast mannan and the monomeric sugars l-f ...19836841579
diarrhoea associated with clostridium difficile in a hospital population.the incidence of disease associated with clostridium difficile was investigated in a general hospital population over a period of six months. in 26 (14.5%) of 179 patients studied, c. difficile was either isolated or faecal cytotoxin was detected. the incidence of other enteropathogenic bacteria, except aeromonas hydrophila, was low. faecal cytotoxin was not detected in nine patients (35%), and non-cytotoxigenic strains of c. difficile were isolated from these patients. in seven patients, a sele ...19836843465
enterotoxigenicity, hemagglutination and cell-surface hydrophobicity in aeromonas hydrophila, a. sobria and a. salmonicida.thirty-one aeromonas hydrophila, 13 a. sobria and two a. salmonicida strains of diverse sources were tested for enterotoxigenicity, hemagglutination and cell surface hydrophobicity. although 93% of the culture supernatant fluids of the aeromonas strains exhibited cytotoxic effects on y1 adrenal and chinese hamster ovary (cho) cells, typical rounding of y1 adrenal cells was reproducibly observed before cytotoxicity for 80% of the isolates within 1 h of exposure. twenty-eight strains were positive ...19836845633
microbiology problem. 19836846380
structural investigations on the core oligosaccharide of aeromonas hydrophila (chemotype ii) lipopolysaccharide. 19836850675
phenotypic markers associated with gastrointestinal aeromonas hydrophila isolates from symptomatic children.aeromonas hydrophila gastroenteritis was detected in 12 pediatric patients during a 5-month period. chief complaints included bloody diarrhea, fever, vomiting, and abdominal pain. severe symptoms in two patients necessitated hospitalization and supportive care. phenotypic characteristics associated with enterotoxigenicity of a. hydrophila strains demonstrated that all 12 isolates were cytotoxic to hela cells and most were lysine decarboxylase positive (75%). a correlation existed between the pre ...19836853687
effects of storage on microbial loads of two commercially important shellfish species, crassostrea virginica and mercenaria campechiensis.the effects of storage on the microbial load in two commercially important species of shellfish were examined. oysters (crassostrea virginica) were stored as shellstock, shucked meats, and fully processed meats at four temperatures for up to 21 days, and clams (mercenaria campechiensis) were stored only as shellstock. the concentrations of most microbiological groups of organisms increased with the duration and temperature of storage in both shellfish species, although the increases were signifi ...19836859844
hydroxamates and aliphatic boronic acids: marker inhibitors for aminopeptidase. 19836860652
[aeromonas hydrophila septicemia in a multiple myeloma patient]. 19836867400
purification and characterization of the cell surface virulent a protein from aeromonas salmonicida.the predominant cell surface protein (a protein) of aeromonas salmonicida has been purified in high yield from outer membranes by using a combination of detergent and chaotropic extraction methods as well as exclusion and ion-exchange chromatography. the a protein was primarily monomeric, mr 50 000, but readily formed oligomers at high protein or low salt concentrations. several isoelectric forms were distinguishable with purified protein as well as in situ on the cell surface. neither phosphate ...19836871174
numerical taxonomy of vibrio cholerae and related species isolated from areas that are endemic and nonendemic for cholera.a total of 165 strains of vibrios isolated from clinical and environmental sources in the united states, india, and bangladesh, 11 reference cultures, and 4 duplicated cultures were compared in a numerical taxonomic study using 83 unit characters. similarity between strains was computed by using the simple matching coefficient and the jaccard coefficient. strains were clustered by unweighted average linkage and single linkage algorithms. all methods gave similar cluster compositions. the estimat ...19836874901
minimal inhibitory concentrations of antimicrobial agents against aeromonas hydrophila determined with the autobac mts.minimal inhibitory concentrations of seven antimicrobial agents for 22 strains of aeromonas hydrophila were determined with the autobac mts procedure. the ranges of the minimal inhibitory concentrations were found to be similar to those obtained by a microdilution procedure. depending upon the interpretive criteria used, there were discrepancies with carbenicillin, cephalothin, kanamycin, and tetracycline. it is recommended that the beta-lactam antibiotics ampicillin, carbenicillin, and cephalot ...19836874911
faecal carriage rate of aeromonas hydrophila.over a four and a half month period, 1004 unselected routine faecal specimens from 815 patients were cultured for aeromonas hydrophila. forty-two specimens (4.2%) representing 38 patients were culture-positive. the study specimens also yielded salmonella on 116 occasions, shigella on seven, campylobacter species on six and other bacterial pathogens on 17 occasions, respectively. seven specimens had a hydrophila together with another bacterial pathogen. in only 19 of 38 patients (50%) was a hydro ...19836875018
aeromonas hydrophila wound infection of the hand initially presenting as clostridial myonecrosis.a traumatic hand laceration in a previously health 17-year-old boy resulted in a rapidly progressive palmar space infection, with gas formation, in the soft tissues caused by aeromonas hydrophila. typically associated with aquatic injuries, a. hydrophila was not initially suspected since the injury did not occur near water. physicians treated trauma-related soft tissue infections, particularly when the initial gram stain shows many polymorphonuclear leukocytes and gram-negative bacilli, must con ...19836875242
one hundred fold increased activity of aeromonas aminopeptidase by sequential substitutions with ni(ii) or cu(ii) followed by zinc.full substitution of cu(ii) or ni(ii) for the two g-atom zinc in aeromonas aminopeptidase hyperactivates the enzyme 6.5 and 25 fold respectively. even greater enhancements of activity can be achieved with mixed metal substitutions. thus, apoenzyme reactivated by first adding one g-atom zinc followed by one g-atom of either cu(ii) or ni(ii) is 15 and 22 times more active than the native enzyme. reversing the order, i.e. by first adding either one g-atom cu(ii) or ni(ii) followed by one g-atom zin ...19836882446
serological diagnosis of diarrhoea caused by some representatives of the genera vibrio and aeromonas.various serological methods were used to study 630 blood sera of patients with diarrhoea on whom microorganisms of the genera vibrio (vibrios of non-01 group, phosphorescent, parahaemolytic and alginolytic vibrios) and aeromonas were isolated, and also of patients with unconfirmed bacteriological diagnosis, carriers and healthy persons. it has been established that specific antibodies are isolated in 30-50% of cases, most frequently in the agglutination reaction, in phar with flagellar erythrocy ...19836886416
the microbiology of childhood gastroenteritis: aeromonas species and other infective agents.a prospective, 12-month study of 975 non-aboriginal children with diarrhea and age- and sex-matched children without diarrhea, in perth, western australia, was designed to investigate the significance of enterotoxigenic aeromonas species as a cause of diarrhea. enterotoxigenic aeromonas species were found in the fecal specimens of 10.8% of the patients with diarrhea but in only 0.7% of those without diarrhea. most aeromonas species were isolated during the summer. other important bacterial patho ...19836886488
microbial growth associated with granular activated carbon in a pilot water treatment facility.the microbial dynamics associated with granular activated carbon (gac) in a pilot water treatment plant were investigated over a period of 16 months. microbial populations were monitored in the influent and effluent waters and on the gac particles by means of total plate counts and atp assays. microbial populations between the influent and effluent waters of the gac columns generally increased, indicating microbial growth. the dominant genera of microorganisms isolated from interstitial waters a ...19836625567
aeromonas hydrophila myonecrosis and gas gangrene in a nonimmunocompromised host.aeromonas hydrophila myonecrosis with gas gangrene and bacteremia developed in an elderly patient after minor trauma from a fishbone. despite aggressive medical and surgical therapy, she experienced a rapidly fatal outcome. literature on aeromonas muscle infection is reviewed and implications for therapy are discussed.19836625789
the role of aeromonas in acute diarrhoeal diseases in nigeria. 19836627379
differential susceptibility of aeromonas hydrophila and enterobacteriaceae to nalidixic acid.the susceptibility of 49 strains of aeromonas hydrophila and 77 strains of enterobacteriaceae towards nalidixic acid was compared by tube dilution and agar diffusion methods. a higher susceptibility was exhibited by aerom. hydrophila and no overlap in mics was found for strains of the two groups of microorganisms. discs containing 0.25 microgram of nalidixic acid produced measurable zones of growth inhibition with aerom. hydrophila and no zones with the enterobacteriaceae. the use of a disc with ...19836630110
acquisition of iron by aeromonas salmonicida.the ability of six typical and three atypical strains of aeromonas salmonicida to sequester fe3+ from the high-affinity iron chelators ethylenediaminedihydroxy-phenylacetic acid, lactoferrin, and transferrin was determined. typical strains were readily able to sequester fe3+ and used two different mechanisms. one mechanism was inducible and appeared to involve production of a low-molecular-weight soluble siderophore(s). iron uptake by this mechanism was strongly inhibited by ferricyanide. one vi ...19836630151
[antibiotic sensitivity of bacteria of the family vibrionaceae isolated from the intestines of swine].bacteria of aeromonas hydrophila subsp., biotype 1 and aeromonas anaerogenes, biotype 2, as well as proteolitica of the vibrio pasahumoliticus biotype were isolated from the intestine of pigs with diarrhea. close antibiotic sensitivity of the isolates was shown. the differences in their sensitivity were not sufficient for defining the taxonomic features.19836638971
characterization of immunoglobulins from the brown bullhead (ictalurus nebulosus) produced against a naturally occurring bacterial pathogen, aeromonas hydrophila.the primary immune response in the brown bullhead (ictalurus nebulosus) to intramuscularly injected, chloroform-killed aeromonas hydrophila reached an agglutination titer of 128 in 3 wk, and the response to the bacteria plus adjuvant reached a titer of 512 in 5 wk. anti-aeromonas hydrophila antibodies from brown bullheads were excluded from sephadex g-200 and had an immunoelectrophoretic migration pattern toward the cathode. cleavage of the macromolecule by 2-me reduction and iodoacetamide alkyl ...19836642044
microbiological & clinical studies in diarrhoea due to plesiomonas shigelloides. 19836642576
panophthalmitis and otitis interna in fire-bellied toads.microbiologic and histologic studies were made of fire-bellied toads with signs of ocular and central nervous system disease. providencia alcalifaciens, citrobacter freundii, aeromonas hydrophila, and other gram-negative bacilli were isolated from the eyes and multiple tissues of ill toads. the histologic evaluations revealed severe panophthalmitis and otitis interna.19836643232
stereospecificity of amino acid hydroxamate inhibition of aminopeptidases.hydroxamates of amino acids and aliphatic acids are effective inhibitors of aeromonas proteolytica amino-peptidase (ec 3.4.11.10) and of both the cytosolic (ec 3.4.11.1) and microsomal (ec 3.4.11.2) aminopeptidases of swine kidney. cytosolic leucine aminopeptidase and the aeromonas enzyme were inhibited to a greater extent by d isomers than by the l enantiomorphs, manganese-activated kidney cytosolic leucine aminopeptidase being inhibited 10 times more effectively by d-leucine and d-valine hydro ...19836643439
correlation of enterotoxicity with biotype in aeromonas spp.enterotoxin production correlated with biotype in a study of 686 strains of aeromonas spp. from indonesia, thailand, the united states, and western australia. most strains were isolated from feces but nonfecal human isolates and environmental strains were also included. more than 80% of voges-proskauer (vp)-positive strains, classified as a. hydrophila, were enterotoxigenic in the suckling mouse assay as were 90% of vp-positive, arabinose-negative strains. an association between positive vp, ara ...19836643669
[taxonomic study of free nitrogen-fixing bacteria isolated from the endorhizosphere of rice].twenty strains of free-living n2-fixing bacteria, isolated from the endorhizosphere of rice in rice soils of senegal, were studied on the basis of 259 morphological, physiological, biochemical and nutritional characters. half of them were gram-negative small rods with polar flagella and showing a strictly respiratory metabolism; they were characteristic of the genus pseudomonas. a first group of 6 strains was related to the p. cepacia-p. marginata group characterized by lophotrichous flagella; t ...19836651126
comparative in vitro activities of ten antimicrobial agents against bacterial enteropathogens.the in vitro susceptibilities of 50 strains of salmonella spp., 80 strains of shigella spp., and 50 enterotoxigenic escherichia coli, 14 yersinia enterocolitica, 6 aeromonas hydrophila, 4 plesiomonas shigelloides, 9 vibrio parahaemolyticus, and 30 campylobacter jejuni strains that were recently isolated from worldwide sources were determined for 10 antimicrobial agents. the antimicrobial agents tested included ampicillin, bicozamycin, doxycycline, enoxacin (ci-919), erythromycin, furazolidone, a ...19836651278
presence of a new cytochrome b - like pigment with a peak at 567 nm in various aerobic bacteria.several physiological groups of bacteria were examined for the presence of a cytochrome b - like pigment which is demonstrable in dithionite-reduced minus substrate-reduced difference spectra. this pigment is characterized by an unusually high alpha band at 567 nm, a low concentration relative to conventional cytochromes, and an inability to be fully reduced by endogenous substrates or nadh. previous studies with one denitrifying and nondenitrifying species of the genus pseudomonas, in paracoccu ...19836652580
a numerical taxonomic study of species of vibrio isolated from the aquatic environment and birds in kent, england.a numerical taxonomic study has been carried out to confirm the identity of strains of the family vibrionaceae isolated during an ecological study. a total of 237 strains were studied including 148 from the aquatic environment, 6 from estuarine birds, 1 from sheep faeces, and 61 control cultures. duplicates of 21 of the strains were randomly selected and included to estimate test and operator error. taxonomic resemblance was estimated on the basis of 148 characters using euclidean distance. the ...19836654766
[isolation of plesiomonas shigelloides in a case of gastroenteritis]. 19836658089
distribution of imvc biogroups of aeromonas hydrophila and aeromonas sobria isolated from human, fish and water.aeromonas sobria and a.hydrophila were isolated from infected fish in ratio of 3.5:1 during the outbreak of fish infections from december 1982 to february 1983, while isolates from human diarrheic stool was 1 :2. on the basis of imvc reactions 138 isolates of motile aeromonads could be divided into 11 biogroups, with biogroup 4 showing statistically significant association with infections. nine biogroups of aeromonads which were isolated from infected fish reflected that the outbreak was not cau ...19836658502
bacterial chemotaxis to effluent from a rum distillery in tropical near-shore coastal waters.pseudomonas aeruginosa and vibrio cholerae showed a strong positive chemotactic response towards rum distillery wastewaters (mostos) and a high oxygen uptake rate in the presence of this complex substrate. rum slops stimulated only motility in aeromonas hydrophila and escherichia coli. the a. hydrophila and e. coli isolates were unable to oxidize mostos significantly.19836660880
a lipopolysaccharide-specific bacteriophage for aeromonas salmonicida.cell wall lipopolysaccharide (lps) was identified as the receptor for the aeromonas salmonicida bacteriophage strain 55r-1. mutants of a. salmonicida resistant to phage 55r-1 were unable to adsorb phage 55r-1 and were shown to be defective in lps structure. purified a. salmonicida lps inactivated phage 55r-1, but the o-polysaccharide and the core oligosaccharide portions of the lps were ineffective. these results suggest that lipid a was required for receptor activity. antibodies directed agains ...19836661705
[aeromonas hydrophila var. anaerogenes as a probable etiologic factor in food poisoning]. 19836665204
[ecological studies on the microbial flora of raw oysters (crassostrea gigas) harvested in hiroshima and the tohoku district]. 19836668346
enhancement of bacterial adhesion by shear forces: characterization of the haemagglutination induced by aeromonas salmonicida strain 438.application of a viscometric assay to the haemagglutination induced by aeromonas salmonicida strain 438 showed that shear forces can enhance the strength of bacterial adhesion. the d-mannose/l-fucose-sensitive reaction proceeded in two phases, an initial phase in which the degree of aggregation remained constant during shearing and a second stage, induced by shear, in which agglutination was enhanced as shear was maintained. the results strongly paralleled those found in studies of concanavalin ...19836668467
aeromonas species in clinical microbiology: significance, epidemiology, and speciation.over a one-year period, 32 strains (31 clinical, 1 environmental) of aeromonas sp. were recovered. chief sources of isolation were the gastrointestinal tract (48%), wounds (19%), and blood (13%). gastrointestinal isolates were most often recovered from young (less than 5 yrs) children with diarrhea; wound or blood isolates were recovered more often from an older (avg. 56 yrs) population with one of several underlying disorders. regardless of body site of isolation, most strains of aeromonas appe ...19836673896
[evaluation of a new system for the simultaneous identification and antibiotic sensitivity testing of gram-negative bacilli by automation].abac- identibiogramma ii is a new system allowing the carry out of antimicrobial susceptibility testing on various bacterial groups and also the fully automatic simultaneous susceptibility testing and identification of gram-negative bacilli (urinary and not). particularly most species of enterobacteriaceae and aeromonas hydrophila, acinetobacter sp., pseudomonas sp. can be identified. this system has two important characteristics, due to a computerized program: that is the possibility of using s ...19836675045
enterotoxigenic aeromonas hydrophila and diarrhoea in adults.aeromonas hydrophila was isolated from the faeces of 32 patients during a nine month period in three hospitals in western australia. all 32 isolates produced enterotoxin which was detected by the suckling-mouse test, and all patients except one had diarrhoea. treatment should be considered in patients with chronic diarrhoea and in those with malignant disease or with hepatobiliary disease who are at risk of developing aeromonas septicaemia.19836571519
aeromonas hydrophila gastroenteritis. 19836580875
aeromonas hydrophila and plesiomonas shigelloides endophthalmitis. 19836604458
enterobacteriaceae and aeromonas hydrophila in minnesota frogs and tadpoles (rana pipiens).in 222 rana pipiens frogs and 34 tadpoles captured in and near minnesota, aeromonas hydrophila and 29 species of enterobacteriaceae, including yersinia enterocolitica and salmonella arizonae, were isolated from intestines. the prevalence of members of the family enterobacteriaceae was lowest in frogs captured in early spring and highest in frogs captured in late summer.19836607034
[study of aeromonas (motile) in the sewage from the city of buenos aires and water from the río de la plata].the incidence of motile aeromonas in the sewage of buenos aires city and in the water of the río de la plata was investigated. eight morphological and biochemical properties were established for the classification of bacteria in the genus aeromonas. these properties adapted from popoff and veron (19) permitted us to classify the isolated strains into two species: a. hydrophila and a. sobria. in the water of the río de la plata, of 27 isolated strains 24 were classified as a. hydrophila and 1 as ...19836400759
microbiological analysis of rock cod (sebastes spp.) stored under elevated carbon dioxide atmospheres.the numbers and types of microorganisms on fresh rock cod fillets and fillets stored in air or in a modified atmosphere (ma; 80% co(2), 20% air) at 4 degrees c were compared. samples were analyzed after 0, 7, 14, and 21 days of storage. the isolation plates were incubated aerobically, anaerobically, or under ma at 4, 20, or 35 degrees c. after 7 days of storage in air, the fillets were obviously spoiled and had a 3- to 4-log cycle increase in microbial counts. plate counts increased more slowly ...19836405691
investigation of aeromonas isolated from water; a serological study using ouchterlony and immunoelectrophoresis techniques.we have shown that immunoelectrophoresis and the ouchterlony double diffusion technique are valuable assays for the identification of aeromonas species. serological correlations have been found between the antigenic extracts originated from a collection reference strains of aeromonas hydrophila subsp. hydrophila and those originated from wild type water isolated.19836409663
a fishy tale: trout borne aeromonas hydrophila septicaemia. 19836414621
the serological properties of the cell surface proteins of vibrio cholerae.the serological properties of cell surface proteins of vibrio cholerae belonging to both the biotypes (classical and el tor) and the serotypes (ogawa and inaba) were investigated. proteins were isolated by extracting v. cholerae with edta in the presence of sodium chloride. the surface localization of these proteins was confirmed with (a) radioiodinated protein a as an immunoprobe and (b) antiserum absorption studies with whole bacteria. there were similarities among the polypeptides of cell sur ...19836415227
posttraumatic aeromonas hydrophila osteomyelitis.aeromonas hydrophila is a gram-negative organism that is the causative agent in several clinical infections. although it has been reported to cause osteomyelitis in immunocompromised patients, it has not been reported to cause this in the normal host. we describe two patients in whom acute osteomyelitis developed following trauma in freshwater lakes. cultures yielded a hydrophila, and both patients responded to a two-week course of parenteral antibiotics followed by oral tetracycline hydrochlori ...19836416197
enterotoxic activity of aeromonas hydrophila and aeromonas sobria. 19836421948
a longitudinal study of the prevalence of bacterial enteric pathogens among adults with diarrhea in bangkok, thailand.six hundred sixty adults with diarrhea treated at bamrasnaradura hospital, bangkok, thailand were investigated to determine the prevalence, seasonality, and severity of diarrhea associated with bacterial enteric pathogens in 1980 and 1981. shigella were isolated from 27% and vibrio parahaemolyticus from 19% of the patients studied. enterotoxigenic escherichia coli (5%), salmonella (3%), non-01 vibrio cholerae (3%), campylobacter jejuni (1%), and group f vibrio (less than 1%) were isolated from a ...19836370564
use of the api 20e, oxi/ferm, and minitek systems to identify nonfermentative and oxidase-positive fermentative bacteria: seven years of experience.the api 20e (api) system, the oxi/ferm (oxf) system, and the minitek (min) system were used over a seven-year period to identify clinical isolates of nonfermentative bacteria (nfb) and oxidase-positive fermentative bacteria (opfb). a total of 742 nfb and opfb were tested with the api system, 988 with the oxf system, and 918 with the min system. the organisms represented 34 recognized species, nine alpha-numeric designates, and three species of unnamed nfb. results were compared to those obtained ...19836370567
detection of campylobacter jejuni and other potential pathogens in travellers' diarrhoea in bangladesh.to determine the distribution of pathogens associated with travellers' diarrhoea in bangladesh, we have studied 269 expatriates who presented with diarrhoea to our clinic, over a one-year period. patients were interviewed about their history of diarrhoea and a stool specimen examined for parasites and cultured for campylobacter jejuni, shigella sp., salmonella sp., vibrio and aeromonas hydrophila and plesiomonas shigelloides. c. jejuni and shigella sp. were each isolated from 45 patients (17%) a ...19836314488
purification and reconstitution in lipid bilayer membranes of an outer membrane, pore-forming protein of aeromonas salmonicida.we have purified a major outer membrane protein from aeromonas salmonicida. this 42-kilodalton protein shared several physical characteristics with enterobacterial porins in that it was noncovalently associated with the peptidoglycan, it was released from the peptidoglycan in the presence of 0.1 m nacl and sodium dodecyl sulfate, and its mobility on sodium dodecyl sulfate-polyacrylamide gels was dependent on the solubilization temperature before electrophoresis. when added to the aqueous solutio ...19836315674
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