Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
rapid microbiochemical method for presumptive identification of gastroenteritis-associated members of the family enterobacteriaceae.a method for rapid screening of isolates of pathogenic members of the family enterobacteriaceae is described. flow charts are used in conjunction with triple sugar iron agar, o-nitrophenyl-beta-d-galactopyranoside-phenylalanine-motility sulfate screening media, oxidase test, and six rapid biochemical tests, namely, lysine decarboxylase, urease, indole, esculin hydrolysis, malonate, and xylose. this scheme is used to provide an inexpensive but rapid presumptive identification of salmonella, shige ...19854008622
comparison of the cathra repliscan ii, the automicrobic system gram-negative general susceptibility-plus card, and the micro-media system fox panel for dilution susceptibility testing of gram-negative bacilli.a comparative evaluation was done to test the accuracy of the cathra repliscan ii agar dilution system (diagnostic equipment, inc., st. paul, minn.), the automicrobic system with gram-negative general susceptibility-plus card (vitek systems, inc., hazelwood, mo.), and the micro-media fox panel micro broth dilution system (micro-media systems, inc., san jose, calif.) in determining mics of 12 antibiotics for 200 gram-negative bacilli. of the 200 strains tested, 12 isolates did not grow in one of ...19854008625
[gram-negative nonfermenting bacteria in respiratory tract infections]. 19854010380
isolation of aeromonas species from animals. 19854018073
invasiveness of aeromonas spp. in relation to biotype, virulence factors, and clinical features.of 69 fecal isolates of aeromonas spp., 18 had the ability to invade hep-2 cells. invasiveness correlated with biotype; of the 18 invasive strains, 16 were a. sobria and 2 were a. hydrophila. no invasive strains were found among the a. caviae. of the 18 invasive strains, 13 were enterotoxigenic. of the enterotoxigenic and invasive strains, 12 were a. sobria, but enterotoxicity was also more common among noninvasive strains of a. sobria. fucose-resistant hemagglutination was also more common in a ...19854019741
the antagonism of tetracycline and ferric iron in vivo.to test the hypothesis that the in-vivo antibiotic action of tetracycline might be affected by ferric iron and the enhancement of infection by ferric iron by tetracycline, the actions of intraperitoneal antibiotic and local ferric ammonium citrate, given separately and together, were measured in the dorsal skin of guinea-pigs bearing lesions due to staphylococci, streptococci, a proteus sp., an erysipelothrix sp., clostridium perfringens, pseudomonas aeruginosa, aeromonas hydrophila and klebsiel ...19854020849
bacteremia caused by aeromonas species in hospitalized cancer patients.bacteremia caused by aeromonas species occurred in 24 hospitalized patients in a cancer institute during a 13-year period. all but one of these patients had a malignancy (88% had leukemia), and most were receiving chemotherapy for cancer. there was a striking numerical predominance of male patients (82%). unlike some previously described patients with infections due to this organism, none of these 24 patients had recently been exposed to fresh or salt water or to fish. the source of the infectin ...19854023526
a transition-state-analog inhibitor influences zinc-binding by aeromonas aminopeptidase.the transition-state-analog inhibitor, 1-butaneboronic acid, markedly enhances the uptake of one g-atom of zn2+ ions from a metal ion buffer system by zn-depleted aeromonas aminopeptidase. in contrast, a substrate-analog inhibitor, n-valeramide, does not perturb the equilibrium between zn2+ ions and the enzyme in a metal ion buffer system. these results establish a role for metal ions in the binding of 1-butaneboronic acid to aeromonas amino-peptidase and strongly imply that a bound zn2+ ion int ...19854026862
salmonellae and pollution indicator bacteria in municipal and food processing effluents and the cornwallis river.a study was conducted to determine the incidence of fecal coliforms, fecal streptococci, aeromonas hydrophilia, and salmonella spp. in the waste discharges of seven sewage treatment plants, four fruit and vegetable canneries, a meat packing plant, a poultry processing plant, and a potato processing plant located along the cornwallis river in nova scotia, canada. surface water samples were also collected from 13 locations in the river to assess the impact of these waste discharges on the receivin ...19854027779
bacteria in bivalve shellfish with special reference to the oyster.the bacterial flora of the pacific oyster crassostrea gigas, the sea mussel perna viridis and the arkshell clam scapharca cornea differed considerably from that of seawater in both numbers and generic composition. the numbers of heterotrophic bacteria in the bivalve shellfish, including the anaerobes and spore-forming bacteria, were greater than that in the surrounding water. pseudomonas spp. were the dominant organisms, comprising over one third of the 321 strains characterized after isolation ...19854030530
[amino acid composition of white poultry meat contaminated with psychrophilic microorganisms].studied was the effect of psychrophilic organisms of the acinetobacter, aeromonas, alcaligenes, and flavobacter genera on the amino acid composition of white poultry meat stored at 0 degrees to 4 degrees c in the course of 48 hours, and at 18 degrees c in the course of 6 to 12 months. the quantitative determination of individual amino acids was carried out by means of an automatic amino acid analyzer, hitachi type, after the method of speckman, stein, and moore. no changes were established in th ...19854035999
in vitro susceptibilities of aeromonas hydrophila, aeromonas sobria, and aeromonas caviae to 22 antimicrobial agents.mics of 22 antimicrobial agents for 60 strains of three aeromonas species were determined by a microdilution method. the newer cephalosporins such as moxalactam, cefotaxime, and cefoperazone, the aminoglycosides, and chloramphenicol, tetracycline, nitrofurantoin, and trimethoprim-sulfamethoxazole inhibited most of the strains studied. within the genus, a. hydrophila was more resistant than either a. caviae or a. sobria to the antibiotics tested.19854037775
immunity to aeromonas salmonicida in coho salmon (oncorhynchus kisutch) induced by modified freund's complete adjuvant: its non-specific nature and the probable role of macrophages in the phenomenon.juvenile coho salmon (oncorhynchus kisutch), vaccinated with one intraperitoneal injection of formalin-killed virulent aeromonas salmonicida cells suspended in saline, showed increased protection against approximately one ld60 of homologous challenge administered at 30 days post-vaccination. under similar conditions, coho vaccinated with a modified complete freund's adjuvant (mfca) alone were also equally protected. when measured against a more severe a. salmonicida challenge of approximately on ...19854043481
demonstration of cholera toxin-related factor in cultures of aeromonas species by enzyme-linked immunosorbent assay.the production of toxins by aeromonas species was examined by the suckling mouse test, the hemolysin test, and the enzyme-linked immunosorbent assay with anticholera enterotoxin. a factor that was immunologically related to cholera enterotoxin was produced by 5 of 14 strains of aeromonas hydrophila and 4 of 15 strains of aeromonas sobria. analysis by these assays and by a test for heat stability suggested that the factor differed from hemolysin and from toxin that was active in the suckling mous ...19854044041
lack of correlation between known virulence properties of aeromonas hydrophila and enteropathogenicity for humans.five strains of aeromonas hydrophila were selected for use in volunteer challenge trials. all five strains produced cytotoxin, hemolysin enterotoxin, lysine decarboxylase, acetylmethylcarbinol, and dnase. two strains hydrolyzed esculin. all strains produced purulent hemorrhagic fluid accumulation in rabbit ileal loops, but failed to induce keratoconjunctivitis in guinea pigs. none of the strains produced mannose-resistant hemagglutinins. in challenge studies, diarrhea was demonstrated in only 2 ...19854044042
the purification and some properties of h-lysin from aeromonas salmonicida.h-lysin from aeromonas salmonicida has been purified 1770-fold by freeze fractionation, ammonium sulphate precipitation, ion exchange chromatography and gel filtration chromatography. the purified material was predominantly h-lysin, devoid of detectable t-lysin, caseinase or gelatinase activity, although glycerophospholipid: cholesterol acyltransferase (gcat) activity was present. the results suggested that h-lysin and gcat activities were due to different extracellular products. studies of the ...19854045422
clinical predictors of acute bacterial diarrhea in young children.this prospective study assessed the value of presenting history, physical examination, and screening laboratory tests in predicting whether diarrhea in a young child is associated with a stool culture positive for a bacterial pathogen. acutely ill children less than 4 years old were studied in a hospital outpatient setting. two hundred patients were seen in a 9 1/2-month period, which encompassed the seasons of summer, fall, and winter. one hundred ninety-five patients had cultures completed and ...19854047797
[metabolic traits of plesiomonas shigelloides strains isolated from children with acute enteritis]. 19854048753
unfamiliar bacteria isolated from cats. 19854049692
comparison of api 20e, api rapid e, and api rapid nft for identification of members of the family vibrionaceae.sixty isolates, from nine species of the family vibrionaceae, were tested by the api 20e, api rapid e, and api rapid nft systems. results were compared with those obtained with standard biochemicals. included were 7 aeromonas caviae isolates, 27 aeromonas hydrophilia isolates, 10 aeromonas sobria isolates, 3 plesiomonas shigelloides isolates, 3 vibrio alginolyticus isolates, 3 vibrio cholerae isolates, 1 vibrio fluvialis isolate, 5 vibrio parahaemolyticus isolates, and 1 vibrio vulnificus isolat ...19854056003
cephalothin susceptibility as a potential marker for the aeromonas sobria group.fifty-four motile aeromonas strains, composing the three currently recognizable species, were tested for susceptibility to cephalothin by broth dilution and disk agar diffusion assays. cephalothin susceptibility was significantly associated with aeromonas sobria (p less than 0.001) and may be an additional phenotypic marker useful in the identification of this species.19854056009
media for the isolation of aeromonas hydrophila.isolation rates of aeromonas hydrophila from stool samples of symptomatic and asymptomatic individuals were examined for several common enteric media. sheep blood agar with 10 micrograms of ampicillin per ml, preceded by overnight enrichment in alkaline peptone water, yielded 2.6 times the number of isolates as the other media examined and is recommended for the isolation of a. hydrophila from humans.19854056015
recurrent aeromonas hydrophila septicemia, a case report. 19854056647
[aeromonas hydrophila bacteremia. clinical and bacteriological characteristics. apropos of 16 cases]. 19854059610
antibacterial activity of amifloxacin (win 49, 375), a new quinolone agent.the in vitro activity of amifloxacin, a quinolone antimicrobial agent was compared with those of ciprofloxacin, enoxacin, ofloxacin and norfloxacin against gram-positive and gram-negative bacteria. ninety percent of escherichia coli, klebsiella species, aeromonas, salmonella, shigella, citrobacter, enterobacter species, proteus mirabilis, serratia marcescens, and morganella morganii were inhibited by less than or equal to 0.5 microgram/ml. amifloxacin inhibited branhamella, haemophilus, and neis ...19854064609
aeromonas caviae: an enteric pathogen?of the aeromonas spp. isolated routinely in our laboratory from human feces, about two thirds represent aeromonas caviae. in contrast to aeromonas hydrophila and aeromonas sobria, this species has been considered to be of little enteropathogenic significance due to the absence of known virulence factors. the clinical data presented here suggest a clinical significance of a. caviae, at least in some cases. as yet nothing is known about the pathogenic mechanisms involved.19854066046
peritrichous flagella in mesophilic strains of aeromonas.a total of 186 mesophilic strains of aeromonas, comprising 151 a. hydrophila and 35 a. caviae, were tested for peritrichous flagella by leifson's staining method. when incubated on solid medium for 18 hr at 22 c, peritrichous flagella were demonstrated in 28 (18.5%) of the 151 strains of a. hydrophila and in 6 (17.1%) of the 35 strains of a. caviae. the peritrichous flagella in these 34 mesophilic strains of aeromonas were also observed by electron microscopy.19854068347
ticarcillin plus clavulanic acid versus moxalactam therapy of osteomyelitis, septic arthritis, and skin and soft tissue infections.a controlled, randomized study to compare the efficacy and safety of ticarcillin plus clavulanic acid with moxalactam was carried out in 25 evaluable patients with bone, joint, and skin or skin structure infections. of the 13 patients in the ticarcillin plus clavulanic acid-treated group, nine had osteomyelitis, two had septic arthritis, one had cellulitis, and one had a wound infection. four of the 12 moxalactam-treated patients had osteomyelitis, one had septic arthritis, and the other seven h ...19854073082
aeromonas-related diarrhea in adults.we have reviewed the incidence of aeromonas in patients with enteric disease at our hospital and found it to be the highest of any potential enteric pathogen. eighty adult patients with diarrhea had aeromonas isolated from feces, and in 73 aeromonas was the only potential bacterial or parasitic pathogen detected. the spectrum of illness in patients with aeromonas-related diarrhea ranged from acute, self-limited diarrhea to a chronic, indolent diarrheal illness. sixteen percent (13/80) of the pat ...19854074035
prevalence and pathogenicity of aeromonas hydrophila.a field survey to determine the prevalence of aeromonas hydrophila revealed a recovery rate of 8% in 141 specimens derived from a range of live, companion, and exotic avian species. the prevalence rate was similar in 240 sequential postmortem submissions during 1984. studies on the pathogenicity of a. hydrophila showed that 2-to-4-day-old chicks and turkey poults were highly susceptible to exposure via the subcutaneous, yolk-sac, and intracerebral routes, and mortality of 80-100% occurred within ...19854074237
spectral and kinetic studies of metal-substituted aeromonas aminopeptidase: nonidentical, interacting metal-binding sites.apoenzyme prepared by removal of the 2 mol of zn2+/mol from aeromonas aminopeptidase is inactive. addition of zn2+ reactivates it completely, and reconstitution with co2+, ni2+, or cu2+ results in a 5.0-, 9.8-, and 10-fold more active enzyme than native aminopeptidase, respectively. equilibrium dialysis and spectral titration experiments with co2+ confirm the stoichiometry of 2 mol of metal/mol. the addition of only 1 mol of metal/mol completely restores activity characteristic of the particular ...19854074699
antigen localization in the lymphoid organs of carp (cyprinus carpio).a brief morphological description is given of the spleen, head kidney and trunk kidney, the three peripheral lymphoid organs of carp. an antigen-localization study was carried out using aeromonas hydrophila (a cellular bacterial antigen) and an indirect immunofluorescence test. examination of the lymphoid organs at various times after injection (up to 12 months) showed that antigen was at first present in splenic ellipsoids and in solitary phagocytic cells in the spleen, head and trunk kidney. l ...19854075373
an unusual epizootic of anuran aeromoniasis. 19854077645
growth of aeromonas spp. on cefsulodin-irgasan-novobiocin agar selective for yersinia enterocolitica.twenty-eight strains of aeromonas spp. were analyzed for their ability to grow on two different kinds of cefsulodin-irgasan (triclosan; ciba-geigy ag, basel, switzerland)-novobiocin (cin) agar containing 15 or 4 mg of cefsulodin per ml and on inositol-bile salts-brilliant green (ibb) agar. relative to blood agar, 68% of the strains were inhibited by more than 2 logs (i.e., less than 1% growth) at 37 degrees c (39% at 25 degrees c) on cin i (high cefsulodin concentration), 7% were inhibited at ei ...19854077959
holding effects on coliform enumeration in drinking water samples.standard procedures for analyzing drinking water stress the need to adhere to the time and temperature conditions recommended for holding samples collected for microbiological testing. the national drinking water laboratory certification program requires compliance with these holding limits, but some investigators have reported difficulties in meeting them. research was conducted by standard analytical methods to determine if changes occurred when samples were held at 5 and 22 degrees c and anal ...19854083877
[isolation of aeromonas hydrophila in diarrhea. characterization of enterotoxinogenic strains and clinical relations].aeromonas hydrophila is isolated from diarrhoea specimens with increasing frequency. the interest in this organism at the present time is related to the fact that it can produce a number of toxins, in particular alpha and beta cytotoxic haemolysins, an enterotoxin and various enzymes. the authors determined the frequency of isolation of this organism and tested the haemolytic, cytotoxic and enterotoxic effects of culture filtrates in all of the stool specimens received in their laboratory over a ...19854091318
aeromonas hydrophila in chlorinated water supplies.methods for the isolation and enumeration of aeromonas hydrophila in water supplies are described. examination of 286 chlorinated samples collected between july and october showed that 19% of otherwise uncontaminated waters contained aerom. hydrophila, rising to 71% of those from which escherichia coli was isolated. the isolation rate during january and february was only 7% for all samples. on the basis of these results, tap water is a likely source of the aeromonads found in human intestinal fl ...19852859326
polymicrobial aetiology of travellers' diarrhoea.of 35 us peace corps volunteers in thailand, 20 (57%) had a total of 30 episodes of diarrhoea during their first 6 weeks in the country. enteric pathogens were associated with 90% of the episodes. a single pathogen was identified in 17 (57%) episodes, 2-4 pathogens were identified in 10 (33%) episodes, and there were 15 symptomless infections. enterotoxigenic escherichia coli (etec) was identified in 37% of these episodes, and various salmonella serotypes were isolated in 33%. infections with 9 ...19852857430
effect of an activated sludge wastewater treatment plant on ambient air densities of aerosols containing bacteria and viruses.bacteria- and virus-containing aerosols were studied during the late summer and fall seasons in a midwestern suburb of the united states before and during the start-up and operation of an unenclosed activated sludge wastewater treatment plant. the study showed that the air in this suburban area contained low-level densities of indicator microorganisms. after the plant began operating, the densities of total aerobic bacteria-containing particles, standard plate count bacteria, total coliforms, fe ...19852988442
synthesis, export, and assembly of aeromonas salmonicida a-layer analyzed by transposon mutagenesis.suicide plasmid pjb4ji, containing transposon tn5 and phage mu, was introduced into aeromonas salmonicida 449 which produces a surface protein array known as the a-layer. kanamycin-resistant exconjugants of 449 with altered ability to produce the a-layer were selected by virtue of their altered colonial morphology and color on medium containing the dye congo red. analysis of culture supernatants, periplasmic shock fluid, outer membranes, and whole-cell lysates by sodium dodecyl sulfate-polyacryl ...19852993248
aeromonas hydrophila infection in the hand. 19852857697
[epidemiology, etiology and laboratory diagnosis of infectious diarrhea diseases in the tropics].diarrhoeal diseases belong to the leading causes of morbidity and mortality in tropical countries, especially in infants and small children. about one billion episodes are estimated for this group of age with 4.6 million fatalities. many causes are discussed to explain the high incidence: bottle feeding of infants, protein malnutrition, unsafe drinking water and unsafe disposal of excrements and sewage, unsufficient consciousness of personal and domestic hygiene, lack of knowledge on the origin ...19853000920
the slow, tight binding of bestatin and amastatin to aminopeptidases.bestatin reversibly inhibits aeromonas aminopeptidase (ec 3.4.11.10) in a process that is remarkable for its unusual degree of time dependence. the binding of bestatin by both aeromonas aminopeptidase and cytosolic leucine aminopeptidase (ec 3.4.11.1) is slow and tight, with ki values (determined from rate constants) of 1.8 x 10(-8) and 5.8 x 10(-10) m, respectively. in contrast, microsomal aminopeptidase (ec 3.4.11.2) binds bestatin in a rapidly reversible process with a ki value of 1.4 x 10(-6 ...19852865258
the comparative in vitro activity of twelve 4-quinolone antimicrobials against enteric pathogens.the minimal inhibitory concentrations (mics) of twelve 4-quinolone antimicrobials were determined for salmonella typhi (25), salmonella spp. (50), shigella spp. (50), campylobacter jejuni (100), vibrio cholerae (10), vibrio parahaemolyticus (10), yersinia enterocolitica (25), aeromonas hydrophila (25) and plesiomonas shigelloides (10). mics were determined using an agar dilution technique in mueller-hinton agar (oxoid, england) supplemented with 10% lysed horse blood. antibiotic containing plate ...19852941257
nuclease s1 analysis of eubacterial 5s rrna secondary structure.single-strand-specific nuclease s1 was employed as a structural probe to confirm locations of unpaired nucleotide bases in 5s rrnas purified from prokaryotic species of rrna superfamily i. limited nuclease s1 digests of 3'- and 5'-end-labeled [32p]5s rrnas were electrophoresed in parallel with reference endoribonuclease digests on thin sequencing gels. nuclease s1 primary hydrolysis patterns were comparable for 5s rrnas prepared from all 11 species examined in this study. the locations of base-p ...19853001324
molecular cloning and expression of a xylanase gene of alkalophilic aeromonas sp. no. 212 in escherichia coli.a gene coding for a xylanase activity of alkalophilic aeromonas sp. no. 212 (atcc 31085) was cloned in escherichia coli hb101 with pbr322. plasmid pax1 was isolated from transformants producing xylanase, and the xylanase gene was located in a 6.0 kb hind iii fragment. the pax1-encoded xylanase activity in e. coli hb101 was about 80 times higher than that of xylanase l in alkalophilic aeromonas sp. no. 212. about 40% of the enzyme activity was observed in the periplasmic space of e. coli hb101. t ...19852999298
[aquatic contamination of wounds by aeromonas sobria after bathing in the river]. 19853160041
eosinophilic granular cells (egc) and histamine responses to aeromonas salmonicida toxins in rainbow trout.the function of eosinophilic granular cells (egcs) in salmonids is unknown. in a previous study of the pathogenesis of a. salmonicida, injection of crude exotoxins into rainbow trout were shown to reproduce the lesions associated with furunculosis and an accompanying lesion, the dispersion and degranulation of egcs in the intestinal wall was reported. the present study investigated this phenomenon in relationship to the histamine content of the gut. in fish injected i/p with a. salmonicida exoto ...19852410306
electrophoretic and immunochemical analyses of the lipopolysaccharides from various strains of aeromonas hydrophila.sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to analyze the lipopolysaccharides isolated from strains of aeromonas hydrophila which exhibit virulence for fish and which autoaggregate during growth in static broth culture. the lipopolysaccharides contained o-polysaccharide chains of homogeneous chain length. two of the strains produced a surface protein array, and immunofluorescence and phage-binding studies revealed that a number of these o-polysaccharide chains of homogene ...19852413008
use of non-carbonated soft drinks to provide safe drinking water.non-carbonated, low-calorie soft drink concentrates (cordials), when diluted according to manufacturers' instructions, had significant antibacterial effects in vitro. bacteria affected include vibrio cholerae, aeromonas hydrophila, shigella sonnei, salmonella typhimurium and escherichia coli. with vibrios, bacterial counts were reduced from 10(6)/ml to undetectable numbers in less than 10 min. escherichia coli in an initial concentration of 10(6)/ml became undetectable after incubation for 1 h w ...19852409896
aeromonas hydrophila gastroenteritis in tuscany children. 19846739332
[aeromonas hydrophila septicemia. epidemiologic aspects. 15 cases].fifteen cases of aeromonas hydrophila septicaemia, characterized by their frequent pulmonary lesions and the severity of their course, are reported. the delay observed between admission to hospital and first symptoms, together with the presence of anatomical lesions or physiological disturbances suggesting bacterial invasion by the intestinal route, have led the authors to postulate a nosocomial digestive contamination, probably from water, as suggested by the particular ecology of this micro-or ...19846232567
[pathogen distribution in waste water sprinkler irrigation].the spray irrigation with pretreated wastewater was investigated on the sewage farm of the braunschweig sewage utilization association. the emission of airborne bacteria was measured by means of andersen sampler (as), reuter centrifugal sampler (rcs), and sedimentation plates (sp). there was a good correlation of results obtained by parallel measurings of as and rcs. the rcs was more effective by the factor 11.5 than the as sampling airborne microorganisms. however, the as gaves the distribution ...19846377753
immunodominant protein epitopes. i. induction of suppression to hen egg white lysozyme is obliterated by removal of the first three n-terminal amino acids.the lack of response to hen egg white lysozyme (hel) by c57bl (h-2b) mice has been demonstrated previously to be related to the induction of suppressor t (ts) cells which recognize the amino terminal region of hel. in this report, the nature of the protein determinant required for ts cell induction is more precisely detailed using des-1,2,3-hel (ap-hel) prepared with an aminopeptidase purified from aeromonas proteolytica . remarkably, the removal of just these three amino acids obliterates the a ...19846202528
purification and properties of amino acid racemase from aeromonas punctata subsp. caviae.an amino acid racemase, which occurs in the cytoplasmic fraction of aeromonas punctata subsp. caviae, has been purified to homogeneity by the criteria of electrophoresis and ultracentrifugation. the enzyme has a molecular weight of about 80,000 and consists of two subunits identical in molecular weight (about 40,000). the enzyme contains 2 mol of pyridoxal 5'-phosphate per mol of enzyme, and exhibits absorption maxima at 280 nm and 420 nm. the holoenzyme is resolved by dialysis against hydroxyla ...19846427786
vaccination of fish: a practical view.commercial vaccines giving protection against three important bacterial fish diseases (enteric redmouth, furunculosis and vibriosis) became available in the united kingdom in 1983. the degree of protection obtained and the cost effectiveness vary according to the method of presentation (injection, dipping or spraying and oral dosing). these methods and the optimum time for vaccination are described.19846431685
aeromonas spp in travellers' diarrhoea. 19846434027
comparative in vitro activities of apalcillin and piperacillin against gram-negative bacilli.the susceptibilities of 317 gram-negative bacilli to apalcillin and piperacillin were determined by standardized microdilution and disk diffusion tests. the respective percentages of strains susceptible to less than or equal to 64 micrograms of apalcillin and piperacillin per ml were as follows: members of the family enterobacteriaceae, 90 and 88%; randomly selected pseudomonas aeruginosa, 100 and 100%; multi-drug-resistant p. aeruginosa, 100 and 75%; and other nonfermenters and aeromonas hydrop ...19846440476
[spoilage microorganisms encountered in ultra-high temperature processed milk].40 strains of aerobic or facultative anaerobic microorganisms were isolated from a total of 37 spoiled ultra high temperature processed milk. 13 of them were identified as the genus bacillus. they were 6 b. cereus, 5 b. licheniformis, 1 b. brevis and 1 b. pumilus. the other 27 strains were nonsporeforming microorganisms, which included 5 yeasts, 2 pseudomonas sp., 3 streptococcus sp., 12 lactobacillus sp., 1 shigella sp., 1 aeromonas sp. and 3 micrococcus sp. results indicate that the spoilage o ...19846468029
[distribution of enterobacteria in soil following irrigation with waste water].the filtration of wastewater after irrigation by sandy soil in the area of the braunschweig sewage utilization association was investigated. for that purpose, the concentrations of gram-negative aerobe rods were determined in soil samples of 15 cm, 30 cm, 45 cm, 60 cm, 75 cm, 90 cm, 105 cm, 120 cm, 135 cm, and 150 cm depth immediately after an irrigation of about 50 mm wastewater during the vegetation (3 series), the vegetationless period (6 series), after one week (3 series) and after two weeks ...19846475379
[aeromonas hydrophila in ivory coast epidemiologic and ecologic aspects and its enteropathogenic role].a research based on the analysis of 479 coprocultures , allow the isolation of 22 strains of aeromonas hydrophila. the epidemiological aspects, the contamination processes through water, food... and the enteropathogenic role of these aeromonas are discussed. these bacteria seen responsible of digestive disturbances from simple gastric discomfort (nausea, light abdominal pain...) to the actual choleriform syndrome. the isolation of 7 strains from the environment (water from lagune , wells; lagune ...19846327100
nucleotide base sequence of vibrionaceae 5 s rrna.nucleotide base sequences of 5 s rrnas isolated from vibrio vulnificus, vibrio anguillarum, and aeromonas hydrophila were determined. comparisons among these and sequences of 5 s rrnas from other species of vibrionaceae provide information useful in the evaluation of the evolution of bacterial species.19846479333
purification and some properties of aeromonas hydrophila hemolysin.a hemolysin produced by a strain of aeromonas hydrophila isolated from a patient with diarrhea was purified by acid precipitation and quarternary aminoethyl-sephadex chromatography. the molecular weight of the hemolysin was estimated at 50,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and at 48,000 by sephadex g-100 gel filtration. in polyacrylamide gel electrophoresis at ph 4.0 and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the hemolysin migrated as a single b ...19846480102
[an aeromonas hydrophila choleriform syndrome. apropos of a case from the ivory coast].an observation of a case of choleriform syndrome of the aeromonas hydrophila subspecies anaerogenes biotype ii is reported. in a country dealing with a severe comma bacillus under endemo -sporadic from, the case report here could be wrongly diagnosed as cholera.19846327101
biosynthesis of bacterial glycogen: characterization of adenosine diphosphate glucose synthetases from enterobacter hafniae and aeromonas hydrophila.enterobacter hafniae and aeromonas hydrophila adpglucose synthetases were purified approximately 39- and 61-fold, respectively, over the crude extract. both enzymes were heat stable at 60 degrees c in the presence of inorganic phosphate. the molecular weights of both enzymes were approximately 200,000 which are similar to other enteric adpglucose synthetases studied. based on kinetic results obtained from the partially purified enzymes, the e. hafniae enzyme is activated twofold by phospho-enolp ...19846331331
resistance of aeromonas hydrophila to beta-lactam antibiotics.the activity of some beta-lactam antibiotics upon 20 strains of aeromonas hydrophila and some properties of their beta-lactamases have been studied. high degree of resistance to benzylpenicillin and lower resistance to ampicillin and cephaloridine was observed. clavulanic acid showed the highest activity. when clavulanic acid was assayed at subinhibitory concentrations in association with ampicillin. mics of the latter decreased to two- to eight-fold. all strains produced inducible beta-lactamas ...19846335147
isolation of enterotoxigenic, hemolytic, and antibiotic-resistant aeromonas hydrophila strains from infected fish in bangladesh.strains of aeromonas hydrophila isolated from skin infections of common freshwater fish in bangladesh were tested for enterotoxin production, hemolysin production, and any correlation between these two activities. we also tested the resistance patterns of a. hydrophila to different drugs, especially in relation to ampicillin. the a. hydrophila strains produced an enterotoxin that was related to their beta-hemolytic activities. production of beta-hemolysin may thus be an indicator of enterotoxici ...19846508295
plasmid coding for transferable drug resistance in bacteria isolated from cultured rainbow trout.the occurrence of drug resistance and plasmid-mediated transferability was investigated in 170 strains belonging to eight bacterial groups isolated from cultured rainbow trout. it was found that 87.6% of the strains were resistant to at least one drug, with the highest percentages of resistance being detected for ampicillin (54.7%), sulfadiazine (46.5%), nitrofurantoin (38.2%), and chloramphenicol (37.0%). six enterobacteria, two vibrio, and one aeromonas isolate transferred resistance factors t ...19846508296
aeromonas septicemia with shock in children with thalassemias. 19846512449
value of blood agar for primary plating and clinical implication of simultaneous isolation of aeromonas hydrophila and aeromonas caviae from a patient with gastroenteritis.the simultaneous recovery of aeromonas hydrophila and aeromonas caviae from the stool of a 49-year-old woman with watery diarrhea was facilitated through the use of a blood agar medium which detected the hemolytic capability of a. hydrophila. in vitro phenotypic tests support the conclusion that only the a. hydrophila isolate was clinically significant.19846520231
surface layer virulence a-proteins from aeromonas salmonicida strains.superficial surface layer proteins (a-proteins) were present on diverse isolates of aeromonas salmonicida which differed both physiologically and in pathogenesis. three of these proteins were purified directly from the surface of whole cells or from outer membrane preparations. these a-proteins were unusually hydrophobic (45-47%) and of similar but not identical molecular mass (49, 50, and 51 kdaltons). they were nearly identical in amino acid composition and were highly conserved, but not ident ...19846525563
phylogenetic relationships among vibrio anguillarum plasmids.results of restriction endonuclease analysis and southern blot hybridization suggest that the r-plasmids from vibrio anguillarum strains isolated in japan can be divided into at least four groups of homology depending on the time of their isolation and geographical source. molecular cloning experiments allowed identification of specific restriction endonuclease fragments carrying the genes for either cmr or tcr as the common sequences between some of these groups of r-plasmids. the cmr region fr ...19846528000
aeromonas hydrophilia tenosynovitis in an immunocompromised host. 19846536592
[partial purification of the extracellular proteolytic system of aeromonas hydrophila lp50: comparative chromatographic and electrophoretic study].a complex extracellular proteolytic system was produced by aeromonas hydrophila lp50 on glucose- polypeptone medium. partial purification of this system was accomplished by ammonium sulphate precipitation, acetone precipitation, gel filtration on sephacryl- s200 and chromatography on deae-sephacel. every stage was controlled by electrophoresis. this proteolytic system was constituted of three aminopeptidase and two endopeptidase components.19846372579
[aeromonas hydrophila: epidemiological study in the area around rome]. 19846536924
neuraminidase production by vibrio cholerae o1 and other diarrheagenic bacteria.vibrio cholerae o1 strains belonging to both biotypes (classical and el tor) and both serotypes (ogawa and inaba) produced neuraminidase which was released rather than cell bound. classical strains made more neuraminidase than did el tor strains. about one-third of v. cholerae non-o1 strains and one-fourth of aeromonas hydrophila strains were neuraminidase positive. strains of enterotoxigenic escherichia coli, vibrio parahaemolyticus, and shigella spp. did not produce detectable neuraminidase.19846373618
effects of short-term cold storage on recovery of proteases from extracellular products of aeromonas hydrophila.three protease-containing fractions were recovered by gel filtration from concentrated crude extracellular products produced by aeromonas hydrophila grown in a defined medium. the recovery of a heat-stable protease was differentially prevented when the crude preparation was stored for 48 h at -20 degrees c but was unaffected by storage of the crude preparation at either 4 or -70 degrees c. once fractionated, the heat-stable protease appeared to be unaffected by subsequent storage at 4, -20, or - ...19846385854
the impact of terrestrial and estuarial factors on the density of environmental bacterial (vibrionaceae) and faecal coliforms in coastal water.a study of the densities of faecal coliforms, vibrio anguillarum, vibrio alginolyticus and aeromonas hydrophila in danish bathing areas was carried out over the period may-october, 1981 with special reference to specific terrestrial and estuarial determinants. significant correlations were found between v. anguillarum and v. alginolyticus (r = 0.57) and a. hydrophila and faecal coliforms (r = 0.43). the densities of the vibrios were highly influenced by temperature and distance from pollution so ...19846395571
furunculosis in rainbow trout (salmo gairdneri) raised in sea water.ulcerative skin lesions were encountered in rainbow trout raised in sea water by a commercial concern in the western cape, south africa. grossly, the lesions resembled furunculosis but, histopathologically, they differed from typical furunculosis in that bacterial colonies were rarely found in the organs, and also the kidneys and spleens were minimally involved. the causative organism was identified as an achromogenic aeromonas salmonicida that shared characteristics with all 3 subspecies, salmo ...19846493730
aeromonas: biology of the organism and diseases in children. 19846374630
bacteria isolated from lymph nodes of california slaughter swine.lymph nodes from 280 slaughter swine from 9 california ranches were examined for the presence of aerobic microflora. genera of interest which were isolated (and percentage of animals from which they were isolated) included salmonella (4.3%), beta-hemolytic streptococci (8.2%), mycobacterium avium-complex (6.4%), corynebacterium pyogenes (0.4%), and aeromonas hydrophila (5.7%). patterns of bacterial isolations from swine herds may be of assistance in predicting herd health problems.19846383150
isolation of aeromonas hydrophila from a metropolitan water supply: seasonal correlation with clinical isolates.the occurrence of aeromonas spp. in the metropolitan water supply of perth, western australia, australia, was monitored at several sampling points during a period of 1 year. water within the distribution system conformed to international standards for drinking water but contained aeromonas spp. in numbers comparable to those in raw surface water, although this water was free of escherichia coli. coliforms and e. coli were found in raw surface waters, and aeromonas spp. were found in raw water fr ...19846385848
aeromonas. 19846389407
aeromonas hydrophila and plesiomonas shigelloides as causes of intestinal infections.infections due to aeromonas hydrophila and plesiomonas shigelloides in immuno-compromised hosts have been well documented, but the role of these organisms in causing gastrointestinal disease in healthy persons is controversial. despite difficulties in characterizing the exotoxins produced by aeromonas species, there is accumulating evidence that these bacteria are capable of causing usually mild, self-limited diarrheal disease in previously healthy adults. some pediatric patients may have protra ...19846390638
aeromonas spp in diarrhoea. 19846437556
an unusual source of hospital wound infection. 19846440623
difference in the extracellular products of two strains of aeromonas hydrophila virulent and weakly virulent for fish.in this study we first compared the toxigenic profile of aeromonas hydrophila strains virulent and weakly virulent for rainbow trout. the separation of the toxic factor for fish is also described. both strains produced hemolytic, enterotoxic, and dermonecrotic activities; the weakly virulent strain produced 20 times more hemolysin. only the cell-free supernatant of the virulent strain produced a toxic factor for fish. after passage on sephacryl s-200, the toxic factor for fish was separated from ...19846478313
aeromonas infection in an infant with aplastic anemia. 19846494020
cloning of enterotoxin gene from aeromonas hydrophila provides conclusive evidence of production of a cytotonic enterotoxin.culture filtrates of two aeromonas hydrophila strains which were isolated from patients with diarrhea and assumed to be causative agents of the infections were shown to contain enterotoxic, cytotoxic, and hemolytic activities. modest heat treatment of the filtrates inactivated the cytotoxic and cytolytic activities, but not the enterotoxic activity. the construction of cosmid gene banks in escherichia coli of dna from both a. hydrophila strains demonstrated that the determinants of the three act ...19846500697
production of cholera-like enterotoxin by aeromonas hydrophila.a total of 249 strains of mesophilic aeromonas including 179 a. hydrophila and 70 a. caviae were tested for production of cholera-like enterotoxin by reversed passive latex agglutination (rpla) assay. a cholera-like enterotoxin neutralized with cholera antitoxin was demonstrated in the culture filtrates from eight (4.5%) of the 179 a. hydrophila strains, while none of a. caviae strains revealed the enterotoxin production in the test. production of the cholera-like enterotoxin in the eight strain ...19846503026
isolation of aeromonas spp. from an unchlorinated domestic water supply.the recovery of aeromonas spp. from the unchlorinated water supply for a western australian city of 21,000 people was monitored at several sampling points during a period of 1 year. membrane filtration techniques were used to count colonies of aeromonas spp., coliforms, and escherichia coli in water sampled before entry to service reservoirs, during storage in service reservoirs, and in distribution systems. aeromonas spp. were identified by subculture on blood agar with ampicillin, oxidase test ...19846486783
the occurrence of aeromonas hydrophila in avian diagnostic submissions.twenty isolations of aeromonas hydrophila were made from 15 species of free-living, commercial, and companion birds submitted for routine diagnostic postmortem examination. seventy percent of the isolations were made from november through march during the 25-month survey period.19846487200
structure of the lipopolysaccharide core isolated from a human strain of aeromonas hydrophila.lipopolysaccharide was isolated from the cell-walls of a human strain of aeromonas hydrophila by the aqueous phenol method in 0.58% yield (based on dry weight of bacteria). the lipopolysaccharide consisted of sr-polysaccharide, core-oligosaccharide and lipid a; there was no o-specific polysaccharide. the core had the composition d-galactose, d-glucose, d-glycero-d-manno-heptose, l-glycero-d-manno-heptose and d-glucosamine in a molar ratio of 1:1:2:4:1. glucosamine was linked to an l-glycero-d-ma ...19846489347
action of a microbial glycerophospholipid:cholesterol acyltransferase on plasma from normal and lcat-deficient subjects.the action of a bacterial acyltransferase similar in overall reaction mechanism to the plasma enzyme lecithin:cholesterol acyltransferase (lcat) has been studied using normal plasma and lipoproteins and plasma from lcat-deficient patients. the microbial enzyme (gcat) catalyzed acyl transfer using phosphatidylcholine and cholesterol in all of the lipoprotein fractions, presumably because it has no apolipoprotein cofactor. in addition, the enzyme was capable of hydrolyzing cholesteryl ester in lip ...19846491538
aeromonas toxins. 19846494019
hemagglutination patterns of aeromonas spp. in relation to biotype and source.aeromonas spp. show patterns of hemagglutination with human group o cells in the presence of fucose, galactose, and mannose. these patterns are related to biotype as well as to the source of isolates. there was good correlation between hemagglutination pattern and the presence of diarrhea among strains isolated in western australia, which was the only source with adequate data for classification of children with an without diarrhea. most of the environmental and other nonfecal isolates produced ...19846690466
biotyping of aeromonas isolates as a correlate to delineating a species-associated disease spectrum.a group of 147 aeromonas isolates from diverse clinical and environmental sources was subjected to the biotyping scheme of popoff and veron. of the 147 isolates biotyped, 137 (93%) could be identified, with aeromonas hydrophila predominating (48%) and equal percentages (25 to 27%) of the other two species (aeromonas sobria and aeromonas caviae). a number of additional biochemical properties were found to be significantly associated with one or more of these three species. these included lysine d ...19846690467
[aeromonas hydrophila septicemia: 3 cases, 1 with mediastinitis].three cases of aeromonas hydrophila septicaemia are reported. the first case concerned a 51 years old patient suffering from drowning who had previously been in good health and who died after a few days of refractory hypoxaemia. the second case was an 8 years old child with trisomy 21 operated for a congenital cardiac defect. the portal of entry was a catheter and the septicaemia was complicated by a. hydrophila mediastinitis, a complication which has not been described previously. the third cas ...19846476665
aeromonas hydrophila colitis in a child.a 5-year-old girl presented with chronic bloody diarrhea. evaluation including sigmoidoscopy, rectal biopsy, and barium enema was consistent with the diagnosis of ulcerative colitis. culture of the stool grew aeromonas hydrophila. a. hydrophila colitis may be more common than presently realized.19846502383
observations of bacterial diseases of captive snakes in finland.eighteen captive, nonpoisonous snakes which suffered from bacterial infections were studied. the most usual bacteria found were gram-negative rods; especially pseudomonas aeruginosa, aeromonas hydrophila and proteus spp. the most common disorders caused by them were stomatitis, septicaemia, bronchopneumonia and abscesses. the sensitivities of ten isolated bacteria to antimicrobial drugs were tested. neomycin and gentamicin seemed to be effective, especially against p. aeruginosa.19846728671
Displaying items 4501 - 4600 of 5519