Publications

incidence and virulence of aeromonas species in feces of children with diarrhea.	aeromonas spp. occurring in feces of children with diarrhea were studied. forty-eight strains were isolated from 2,025 specimens during a one year period. only 11 of 44 strains tested yielded virulence factors (cytotoxin, hemolysin and hemagglutinin). six strains were identified as aeromonas sobria and five as aeromonas hydrophila. the other strains isolated were identified as aeromonas caviae. the biochemical characteristics associated with virulence factors were a positive voges-proskauer reac ...	1986	3743554
[isolation and identification of aeromonas hydrophila from diarrhea patients and its pathogenicity].		1986	3719695
bacteria in enteric lesions of horses.	thirty-three species of bacteria were isolated from the gastrointestinal mucosa of 23 adult horses and two foals. the bacteria isolated could be related to gross and microscopical lesions in some cases. clostridium perfringens type a, actinobacillus equuli, salmonella typhimurium and campylobacter coli biotype 1 could all be associated with gastrointestinal lesions. c jejuni biotype 1 and aeromonas hydrophila were both recovered in this study and have been identified as causes of enteritis in ho ...	1986	3716108
growth of aeromonas species on enteric agars.	the efficacy of eight routine enteric agars for supporting the growth of 32 strains of aeromonas spp. (17 a. hydrophila strains, 8 a. sobria strains, and 7 a. caviae strains) was investigated. the plating efficiency of aeromonas spp. on these media varied greatly (range, 0 to 100%), as did their colony size when compared with that on noninhibitory medium (5% sheep blood agar). plating efficiency on seven of these eight media appeared to be strain- and not species dependent. overall, eosin-methyl ...	1986	3711297
[treatment of neurosurgical bacterial meningitis using the combination of ceftriaxone-fosfomycin].	16 patients with bacterial meningitis following a neurosurgical procedure were given a combination of ceftriaxone and fosfomycin. 8 microorganism were isolated: 2 staphylococcus epidermidis, 1 staphylococcus aureus, 1 neisseria meningitidis, 1 streptococcus pneumoniae, 1 haemophilus influenzae, 1 serratia marcescens and 1 aeromonas hydrophila. no pathogen was identified in the remaining cases. all of the isolated strains were susceptible to both antibiotics. in vitro, neither synergy nor antagon ...	1986	3534726
diarrhoea associated with aeromonas hydrophila.		1986	3570383
microbial filtrates activate granulocytes without complement or prostaglandins.	cardiorespiratory dysfunction in sepsis may be mediated by circulating complement, activated leukocytes, prostaglandins, or by a direct effect of endotoxin. the purposes of this study were to determine if pathogenic microbes produce these substances and to evaluate the direct effects of substances released by micro-organisms on granulocyte aggregation (ga). escherichia coli, (e. coli), aeromonas hydrophila (aeromonas h.), staphylococcus aureus (s. aureus), and candida albicans, (candida a.) were ...	1986	3524892
aeromonas hydrophila: its isolation from acute diarrhoeal illness in rural bangladesh.		1986	3566684
aeromonas salmonicida from salmonids and cyprinids--serological and cultural identification.		1986	3551409
relationship of toxin production to species in the genus aeromonas.	ninety-five strains of aeromonas were divided into three species--a. sobria, a. hydrophila and a. caviae--on the basis of results in 13 biochemical tests. the minimum number of tests necessary to distinguish these species was determined. culture filtrate of the strains were tested for cytotoxin and cytotonin, haemolysin and protease. one filtrate with high-titre cytotoxin, haemolysin and protease activities was subjected to gel filtration on sephadex g75 and isoelectric focussing. of the five ce ...	1986	3540304
effect of beta-lactam prodrugs on human intestinal microflora.	the ampicillin prodrugs bacampicillin, pivampicillin, and talampicillin, the mecillinam prodrug pivmecillinam and the sulbactam prodrug sulbactam pivoxil all have a greatly improved oral availability compared to the parent drug. they show no antibacterial activity themselves until transformed into active drugs after absorption. this double advantage makes them less likely to influence the intestinal microbial ecosystem. ampicillin has been reported to cause marked changes in the colon microflora ...	1986	3547627
antimicrobial activity of imipenem against 1386 clinical isolates.	1386 isolates from clinical specimens were tested against imipenem by disc agar diffusion. the bacteria used in this study consisted of escherichia coli, enterobacter aerogenes, e. agglomerans, e. cloacae, klebsiella pneumoniae, k. oxytoca, k. ozanae, proteus mirabilis, p. vulgaris, providencia stuartii, p. rettgeri, acinetobacter calcoaceticus, citrobacter diversus, c. freundii, morganella morganii, serratia liquefaciens, s. marcescens, hafnia alvei, aeromonas hydrophila, pseudomonas aeruginosa ...	1986	3468319
stress factors that can affect studies of drug metabolism in fish.	the effects of environmental stress conditions on the defense response of rainbow trout following a four week exposure to subacute levels of un-ionized ammonia or temperatures 5 c above and 5 c below the temperature optimum (15 c) were investigated. these experimental studies can serve as a model to evaluate the metabolic response of fish to external agents (e.g., drugs, vaccines) under environmental conditions seen in the culture of fish. blood and tissue immune parameters measured include hema ...	1986	3509652
comparison of the quantum ii bacterial identification system and the automicrobic system for the identification of gram-negative bacilli.	the quantum ii bacterial identification system (bid; abbott laboratories) is a microprocessor-based spectrophotometric system for identification within 4 to 5 h of both enteric and nonenteric gram-negative bacilli. we compared the bid with the automicrobic system (ams; vitek systems, inc.), using the most recent gram-negative identification card and software (ams-gni), for the identification of 501 clinical isolates of gram-negative bacilli, including 382 belonging to the enterobacteriaceae and ...	1986	3517034
cloning, expression, and mapping of the aeromonas hydrophila aerolysin gene determinant in escherichia coli k-12.	dna sequences corresponding to the aerolysin gene (aer) of aeromonas hydrophila ah2 dna were identified by screening a cosmid gene library for hemolytic and cytotoxic activities. a plasmid containing a 5.8-kilobase ecori fragment of a. hydrophila dna was required for full expression of the hemolytic and cytotoxic phenotype in escherichia coli k-12. deletion analysis and transposon mutagenesis allowed us to localize the gene product to 1.4 kilobases of aeromonas dna and define flanking dna region ...	1986	3522552
multiple low-level antibiotic resistance in aeromonas salmonicida.	mutants with multiple low-level antibiotic resistance were isolated from virulent wild-type aeromonas salmonicida strains exposed to a low concentration of any one of several low-molecular-mass (approximately 635 daltons or less) antibiotics. multiple resistance was toward beta-lactam compounds (penicillin g, ampicillin, cloxacillin), quinolones (flumequine, oxolinic acid, nalidixic acid), tetracyclines, chloramphenicol, and novobiocin. susceptibilities of the mutants toward several higher-molec ...	1986	3524440
aeromonas hydrophila peritonitis in a renal transplant recipient.		1986	3540131
synergism and antagonism in double beta-lactam antibiotic combinations.	combinations of two beta-lactam antibiotics may be advantageous in certain clinical situations, providing a synergistic activity against specific organisms or a broad spectrum of antibiotic coverage. depending on the combination and the bacteria, synergism, indifference, or antagonism can be observed. synergism may occur when two beta-lactam antibiotics, acting on different penicillin-binding proteins, are combined or when a penicillinase-susceptible beta-lactam antibiotic is protected by anothe ...	1986	3487247
thromboxane a2 mediates hemodynamic and respiratory dysfunction in graded bacteremia.	thromboxane a2 has been implicated as a mediator of cardiorespiratory dysfunction in sepsis. this study evaluated whether or not thromboxane a2 was necessary or sufficient for these adverse effects to occur during bacteremia. fourteen adult swine under barbiturate anesthesia and breathing room air were monitored with arterial and pulmonary artery catheters. animals were studied for 4 hours in three groups: group i, graded infusion of 10(9)/ml aeromonas hydrophila; group ii, aeromonas hydrophila ...	1986	3526603
gram-negative septicemia in american alligators (alligator mississippiensis).	six species of bacteria (family enterobacteriaceae) not commonly reported as associated with disease in american alligators (alligator mississippiensis) were documented, suggesting that aeromonas is not the only bacterium responsible for septicemia in crocodilians. these included citrobacter freundii, enterobacter agglomerans, proteus sp., morganella morganii, serratia marcescens, and klebsiella oxytoca. clinical signs of disease included intensive basking, anorexia, lethargy, flaccid limb paral ...	1986	3503133
characteristics of aeromonas species and their association with human diarrhoeal disease.		1986	3553304
same day identification of gram-negative rods with the quantum ii microbiology system.	the quantum ii microbiology system was challenged with 479 strains of enterobacteriaceae and 155 strains of nonfermenters (contained in the data base) and aeromonas/plesiomonas. the percentages of strains correctly diagnosed within 5h (i.e., with out additional tests) at greater than or equal to 80% probability were 78% and at greater than or equal to 50% probability, 84.7%.	1986	3512962
the in-vitro activity of ci-934 compared with that of other new 4-quinolones and nalidixic acid.	the in-vitro activity of ci-934, a new 4-quinolone compound, was compared with that of the other new 4-quinolones, enoxacin and ciprofloxacin, and also with that of nalidixic acid. ci-934 was more active than any of the other 4-quinolones tested against gram-positive aerobic organisms including staphylococcus aureus (mics 0.06-0.25 mg/l), beta-haemolytic streptococci (mics 0.12-0.5 mg/l), streptococcus pneumoniae (mics 0.25-0.5 ml/l), viridans streptococci (mics 0.06-0.5 mg/l) and most enterococ ...	1986	3463557
bacteremia due to aeromonas hydrophila: a report of 40 episodes.		1986	3461126
aeromonas infection following facial injury.		1986	3459840
the comparative in-vitro activity of eight newer quinolones and nalidixic acid.	the in-vitro antibacterial activity of nalidixic acid and the 4-quinolones, ciprofloxacin, norfloxacin, enoxacin, ofloxacin, pefloxacin, a-56619, a-56620 and ci-934 was assessed by determination of mics. the 4-quinolones were all highly active against most isolates of enterobacteriaceae, including nalidixic acid-resistant strains. ciprofloxacin (mics 0.002-2 mg/l) was the most active and a-56619 (mics 0.008-32 mg/l) was the least active. a-56619, a-56620, ofloxacin, ciprofloxacin and ci-934 were ...	1986	3468100
beta-lactamases with high activity against imipenem and sch 34343 from aeromonas hydrophila.	two groups of inducible beta-lactamases were found among nine isolates of aeromonas hydrophila. five isolates produced enzymes with high activity against the penem sch 34343 and the carbapenem imipenem: the enzymes from some of these isolates also had activity against penicillin, ampicillin, carbenicillin, cephaloridine and cephalexin but one isolate produced an enzyme with no detectable activity against cephalosporins. the other four isolates produced typical 'cephalosporinases' with activity a ...	1986	3485091
a collaborative evaluation of a rapid, semi-automated identification system for gram-negative bacilli: the quantum ii bid.	the recently-introduced semi-automated bacterial identification system, the quantum ii bid, is designed to identify fermentative and non-fermentative gram-negative bacilli in 4-5 h. the system was evaluated independently by the two participating laboratories. inter-laboratory reproducibility was determined by testing 181 strains in each laboratory and found to be 97.8%. a further 893 organisms, comprising 417 fresh clinical isolates and 476 stock cultures, were then tested by the system in the t ...	1986	3512840
molecular cloning and expression in escherichia coli of the structural gene for the hemolytic toxin aerolysin from aeromonas hydrophila.	the structural gene for the hemolytic toxin aerolysin has been cloned into the plasmid vectors pbr322 and pembl8+. the gene was localized on the hybrid plasmids by analysis of plasmids generated by transposon mutagenesis. the sequence of the first 683 bases of an insert in pembl8+ was determined and shown to encode the amino terminus of the protein as well as a typical signal sequence of 23 amino acids. aerolysin is produced by e. coli cells containing the cloned aerolysin gene and it is process ...	1986	3020368
[diarrhea caused by aeromonas in ivory coast].	the authors drawn epidemiological, clinical conclusions, and the geographical data concerning the diarrheas with aeromonas in ivory-coast. the results relate to a study realized from january 1982 to july 1985. out of the 1,594 excrements 627 germes have been isolated and among which 153 strains of aeromonas if for instance 24.4% of the whole germs. aeromonas caviae is the most frequent species (50%) compared to 27.3% for aeromonas sobria, and 22.7% for aeromonas hydrophila.	1986	3026671
the characterization of a conjugative r-plasmid isolated from aeromonas salmonicida.	we have analyzed two related r-plasmids isolated from aeromonas salmonicida: par32 and pja8102-1, which encode resistance to chloramphenicol, streptomycin, and sulfonamides (su). the genetic map of pja8102-1 indicated that this plasmid consists of at least three functionally different regions. one is the region conferring ability to transfer (tra), one is the region coding for drug resistance genes (r-det), and one is the region encoding the replication functions (rep). both plasmids contain rep ...	1986	3027727
aeromonas in hospital.		1986	2871072
fatal infection with aeromonas sobria and plesiomonas shigelloides.		1986	3081156
[transmission of vibrio cholerae. possible role of the camel].	choleraic vibrio uses the moving means that man discovers to his own use. camels (passive and active vehicles of large quantity of crude water) were experimentally infected with a vibrio (tox. +) and an aeromonas (tox. +). suckling young camels eliminate them for 6 days and grown-up camels for 25 days. this experience, associated with the discovery of vibrio non 01 and shigella in the caravan camel dungs, lead them to become a potential vector of spreading from one oasis to another.	1986	3096512
applicability of coagglutination test for detecting aeromonas salmonicida antigens from extracts of bacterial cultures and experimentally infected tissues of the common carp (cyprinus carpio l.).		1986	2953200
in-vitro activity of newer quinolones against aerobic bacteria.	nalidixic and five newer 4-quinolones, ciprofloxacin, enoxacin, norfloxacin, ofloxacin and pefloxacin were tested against 576 recent clinical aerobic bacterial isolates. the 4-quinolones were regularly active (mic90 less than 4 mg/l) against the following bacteria: staphylococcus aureus, s. epidermidis, s. saprophyticus, different enterobacteriaceae, haemophilus influenzae, campylobacter jejuni, pseudomonas aeruginosa, agrobacter spp., aeromonas spp., plesiomonas spp., neisseria meningitidis. ot ...	1986	2940214
infections of the gastrointestinal tract in the immunocompromised patient.	immunocompromised patients are susceptible to a variety of serious gastrointestinal infections. among the most common or most severe are mycobacterium avium-intracellulare, herpes simplex, candidiasis, cryptosporidiosis, and salmonellosis. some of these infections are difficult or impossible to treat successfully. the correct diagnosis often can only be established if it is first suspected and the appropriate studies are performed.	1986	3010805
pathogens that cause travelers' diarrhea in latin america and africa.	with the advent of rapid and convenient means of transportation, millions of persons travel each year from industrialized to developing countries in the tropics and subtropics. these travelers are at risk for a variety of infectious diseases that are endemic in these areas; the most frequently occurring of these is diarrhea. studies of groups of travelers to latin america and africa have found that approximately one-half develop diarrhea during their stay abroad. etiologic investigations of thes ...	1986	3014633
virulence characteristics of aeromonas spp. in relation to source and biotype.	the significance of aeromonas spp. as potential water-borne enteric pathogens in tasmania, australia, an area with a mild climate and comparatively low year-round water temperatures, was investigated in view of the reported marked peak of aeromonas-associated gastroenteritis in the summer and the apparent influence of temperature on levels of potentially pathogenic species in water supplies. biochemical characteristics and virulence-associated properties--exotoxin production (hemolysin, enteroto ...	1986	2877008
a study of some factors associated with wound infection.	five hundred and four surgical patients were studied at princess basma hospital, north jordan, during the period april 1984-january 1985. the overall postoperative wound infection rate was 3.6%. in clean surgery 1.1% of wounds were infected and in potentially contaminated and contaminated surgery the infection rate was 3.3% and 18.5% respectively. major organisms isolated from infected wounds were staphylococcus aureus (55.5%), escherichia coli (16.6%), proteus vulgaris (11.1%), pseudomonas aeru ...	1986	2878036
aeromonas in hospital--methods of isolation.		1986	2878040
multiplication of bacterial pathogens in intravenous fluids.	the growth of 10 bacterial species in 10% dextran solution, 0.9% saline solution, rheomacrodex solution, totamine solution and extracorporeal circulation fluid was quantified at room temperature. depending on the bacterial species, doubling time ranged from 2 to 18 h. these differences can be explained by microbial metabolism, and the ph and osmotic pressure of the solutions tested. these results emphasize once more the necessity for aseptic preparation and careful storage of intravenous fluids.	1986	2873170
[enteropathogens in travellers returning from the tropics].	diarrhea is not only the most common health hazard during travel in the tropics but also the most frequent condition which prompts returning travellers to see a physician. the prevalence of bacterial and parasitic enteropathogens in people attending our outpatient department after returning from the tropics has been studied and the laboratory results of stool examinations have been compared with clinical symptoms. of the 173 persons enrolled, 19 (11%) harboured bacterial pathogens and pathogenic ...	1986	2875520
ribosomal rna phylogenies for the vibrio-enteric group of eubacteria.	comparisons between the ribonucleotide sequences of 5s rrnas of the gram-negative eubacteria indicate that several families, namely enterobacteriaceae, vibrionaceae and aeromonadaceae possess remarkably similar evolutionary histories. a study of the phylogenetic relationships among these groups, through cluster analysis and construction of evolutionary trees, suggests the existence of dissimilar rates of evolution along the several lineages. these dissimilarities are most evident in comparisons ...	1986	2484672
differential partition of virulent aeromonas salmonicida and attenuated derivatives possessing specific cell surface alterations in polymer aqueous-phase systems.	two-polymer aqueous-phase systems were used to compare via partitioning the surface properties of strains of the fish pathogen aeromonas salmonicida which differed in their ability to produce the surface protein array known as the a layer and in their ability to produce smooth lipopolysaccharide. in these two-phase systems, biological particles are known to partition between the phases in a manner related to a variety of surface properties, including hydrophobicity, charge, and lipid composition ...	1986	2425736
effects of direct immersion in antigen on immunological memory in young carp, cyprinus carpio.	the study asks whether, in fish, antigens encountered early in life can prime the immune system to yield memory responses on subsequent challenge with the same antigen and, if so, whether positive immunity or immunological tolerance is induced. the direct immersion method of vaccination was used to prime 4 week old carp, cyprinus carpio, and was compared with priming by injection. three different forms of antigen were used: the thymus dependent antigen, human gamma globulin (hgg) in soluble and ...	1986	2429432
the ontogeny of humoral immunity in rainbow trout, salmo gairdneri.	the ontogeny of the humoral immune response to a 'thymus dependent' and a 'thymus independent' antigen, human gamma globulin (hgg) and aeromonas salmonicida (as) respectively, was investigated in the fry of rainbow trout, salmo gairdneri, by direct immersion vaccination in the antigens (dose 5 mg/l hgg; 10(8) cells/ml as; 30 minutes) at known ages/weights from 7 days post hatch, and at 1, 2, 3, and 4 months post hatch. half the fry in each group were tested for antibodies 4 weeks after vaccinati ...	1986	2429433
protection of rats against cholera toxin and cholera-like enterotoxins by immunization with enteric-coated cholera toxin.	pure cholera toxin (ct) given as a booster in enteric-coated tablets to rats produced a humoral and intestinal immune response similar to the result of instilling the boosting dose of ct directly into the duodenum. this method protects the antigen against gastric acid and allows delivery of the immunogen to intestinal mucosa, an essential step in producing intestinal secretory iga. immunization gave protection against pure ct during intestinal perfusion but also significantly protected against t ...	1986	2430511
evaluation of different assay systems for identification of environmental aeromonas strains.	[this corrects the article on p. 655 in vol. 51.].	1986	16347094
electrostatic mechanism of survival of virulent aeromonas salmonicida strains in river water.	[this corrects the article on p. 1343 in vol. 51.].	1986	16347195
evaluation of different assay systems for identification of environmental aeromonas strains.	important biochemical reactions in conventional tests were compared with counterpart reactions in two multiple test systems, api-20e (analytab products, plainview, n.y.) and aeromonas hydrophila medium, to evaluate their accuracy for the identification of motile aeromonas spp. isolated from fish. in a total of 49 aeromonas spp. isolates and 10 a. hydrophila reference strains, false-negative or -positive reactions were detected in the voges-proskauer test, indole production, gelatinase activity, ...	1986	16347025
temporal occurrence of vibrio species and aeromonas hydrophila in estuarine sediments.	marine sediments were assayed for their concentration of vibrio spp. and aeromonas hydrophila over 1 year. a temporal variation was observed in which a. hydrophila, and to a lesser degree v. fluvialis, were found in the winter months, v. parahaemolyticus and v. vulnificus predominated in the spring and summer, with non-o-1 v. cholerae and v. alginolyticus detected in the late summer and fall. these organisms were found in greatest numbers in the top 5 cm of sediment, but were detectable down to ...	1985	16346948
significance of extracellular protease for growth of a heterotrophic bacterium, aeromonas salmonicida.	the role of protease produced by a heterotrophic bacterium during growth was investigated with aeromonas salmonicida, the pathogen of fish furunculosis, strain a-7301 and its protease-deficient mutant ntg-1 induced by mutagenesis. strain a-7301 produced extracellular protease in a mixed amino acid medium (composed of gly, ala, val, ile, leu, thr, ser, cys, met, phe, tyr, lys, arg, pro, his, try, asp, asn, glu, and gln at equal concentrations of 0.1 g/liter). its multiplication rate was limited b ...	1985	16346900
starch-ampicillin agar for the quantitative detection of aeromonas hydrophila.	interest in aeromonas hydrophila as a food-borne and human pathogen is increasing. isolation media from the clinical laboratory were evaluated for food use and either did not give quantitative recovery of a. hydrophila or did not permit ready differentiation of a. hydrophila from the background microflora. a new medium was developed which permitted quantitative recovery of a. hydrophila from foods. the medium consisted of phenol red agar base (difco laboratories), soluble starch (10 g/liter), an ...	1985	16346899
eosinophilic granular cells (egc) and histamine responses to aeromonas salmonicida toxins in rainbow trout.	the function of eosinophilic granular cells (egcs) in salmonids is unknown. in a previous study of the pathogenesis of a. salmonicida, injection of crude exotoxins into rainbow trout were shown to reproduce the lesions associated with furunculosis and an accompanying lesion, the dispersion and degranulation of egcs in the intestinal wall was reported. the present study investigated this phenomenon in relationship to the histamine content of the gut. in fish injected i/p with a. salmonicida exoto ...	1985	2410306
electrophoretic and immunochemical analyses of the lipopolysaccharides from various strains of aeromonas hydrophila.	sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to analyze the lipopolysaccharides isolated from strains of aeromonas hydrophila which exhibit virulence for fish and which autoaggregate during growth in static broth culture. the lipopolysaccharides contained o-polysaccharide chains of homogeneous chain length. two of the strains produced a surface protein array, and immunofluorescence and phage-binding studies revealed that a number of these o-polysaccharide chains of homogene ...	1985	2413008
polymicrobial aetiology of travellers' diarrhoea.	of 35 us peace corps volunteers in thailand, 20 (57%) had a total of 30 episodes of diarrhoea during their first 6 weeks in the country. enteric pathogens were associated with 90% of the episodes. a single pathogen was identified in 17 (57%) episodes, 2-4 pathogens were identified in 10 (33%) episodes, and there were 15 symptomless infections. enterotoxigenic escherichia coli (etec) was identified in 37% of these episodes, and various salmonella serotypes were isolated in 33%. infections with 9 ...	1985	2857430
the slow, tight binding of bestatin and amastatin to aminopeptidases.	bestatin reversibly inhibits aeromonas aminopeptidase (ec 3.4.11.10) in a process that is remarkable for its unusual degree of time dependence. the binding of bestatin by both aeromonas aminopeptidase and cytosolic leucine aminopeptidase (ec 3.4.11.1) is slow and tight, with ki values (determined from rate constants) of 1.8 x 10(-8) and 5.8 x 10(-10) m, respectively. in contrast, microsomal aminopeptidase (ec 3.4.11.2) binds bestatin in a rapidly reversible process with a ki value of 1.4 x 10(-6 ...	1985	2865258
aeromonas hydrophila in chlorinated water supplies.	methods for the isolation and enumeration of aeromonas hydrophila in water supplies are described. examination of 286 chlorinated samples collected between july and october showed that 19% of otherwise uncontaminated waters contained aerom. hydrophila, rising to 71% of those from which escherichia coli was isolated. the isolation rate during january and february was only 7% for all samples. on the basis of these results, tap water is a likely source of the aeromonads found in human intestinal fl ...	1985	2859326
aeromonas hydrophila infection in the hand.		1985	2857697
[epidemiology, etiology and laboratory diagnosis of infectious diarrhea diseases in the tropics].	diarrhoeal diseases belong to the leading causes of morbidity and mortality in tropical countries, especially in infants and small children. about one billion episodes are estimated for this group of age with 4.6 million fatalities. many causes are discussed to explain the high incidence: bottle feeding of infants, protein malnutrition, unsafe drinking water and unsafe disposal of excrements and sewage, unsufficient consciousness of personal and domestic hygiene, lack of knowledge on the origin ...	1985	3000920
synthesis, export, and assembly of aeromonas salmonicida a-layer analyzed by transposon mutagenesis.	suicide plasmid pjb4ji, containing transposon tn5 and phage mu, was introduced into aeromonas salmonicida 449 which produces a surface protein array known as the a-layer. kanamycin-resistant exconjugants of 449 with altered ability to produce the a-layer were selected by virtue of their altered colonial morphology and color on medium containing the dye congo red. analysis of culture supernatants, periplasmic shock fluid, outer membranes, and whole-cell lysates by sodium dodecyl sulfate-polyacryl ...	1985	2993248
the comparative in vitro activity of twelve 4-quinolone antimicrobials against enteric pathogens.	the minimal inhibitory concentrations (mics) of twelve 4-quinolone antimicrobials were determined for salmonella typhi (25), salmonella spp. (50), shigella spp. (50), campylobacter jejuni (100), vibrio cholerae (10), vibrio parahaemolyticus (10), yersinia enterocolitica (25), aeromonas hydrophila (25) and plesiomonas shigelloides (10). mics were determined using an agar dilution technique in mueller-hinton agar (oxoid, england) supplemented with 10% lysed horse blood. antibiotic containing plate ...	1985	2941257
humoral response and memory formation in carp after injection of aeromonas hydrophila bacterin.	the humoral immune response of carp (cyprinus carpio) upon a bacterial fish pathogen aeromonas hydrophila was studied in relation to memory formation. after a single intramuscular (i.m.) injection of formalin killed a. hydrophila cells (f-ah), maximum serum ab (ab) titers were observed at day 20. distinct titers were still seen at day 360 in the groups injected with a medium or high antigen (ag) dose (10(7) respectively 10(9) f-ah). the effect of a second immunization with a high ag dose was stu ...	1985	3996709
[spontaneous aeromonas hydrophila peritonitis in the cirrhotic. apropos of 2 cases].		1985	4001643
resistance patterns of nonfermentative gram-negative rods and aeromonads to beta-lactam antibiotics: a diagnostic aid.	consistent resistance to certain antimicrobial agents has in the past been of help in the diagnosis of nonfermentative gram-negative rods. in order to establish patterns of consistent resistance to 15 mostly newer betalactam antibiotics, 561 strains of such organisms plus aeromonas and plesiomonas were tested with the kirby-bauer method and also against the selective agent c-390 in three concentrations in mueller-hinton agar. no species-specific patterns emerged but the resistance data can be us ...	1985	3925660
the in vitro activity and beta-lactamase stability of cefpirome (hr 810), a pyridine cephalosporin agent active against staphylococci, enterobacteriaceae and pseudomonas aeruginosa.	the in vitro activity of cefpirome, a new cyclopyridinium cephalosporin, was evaluated against 947 aerobic and anaerobic bacteria. cefpirome inhibited 90% of escherichia coli, klebsiella spp., citrobacter diversus, morganella morganii, proteus vulgaris, proteus mirabilis, aeromonas spp., salmonella spp., shigella spp. and haemophilus and neisseria species at less than or equal to 0.4 mg/l. it had activity comparable to that of cefotaxime, ceftizoxime, ceftazidime, aztreonam, and moxalactam again ...	1985	3928497
aeromonas hydrophila--an accepted enterotoxigenic "neoenteropathogen"?		1985	3989329
phospholipids and lipopolysaccharide of aeromonas hydrophila.	the phospholipids and lipopolysaccharide of aeromonas hydrophila were characterized. phosphatidylethanolamine and phosphatidylglycerol were the major phospholipid components. the outer membrane contained more phosphatidylethanolamine and less phosphatidylglycerol than the inner membrane, and the phospholipids of the outer membrane contained a higher proportion of saturated fatty acids. only four fatty acids (c14:0, c16:0, c16:1, and c18:1) were found in the phospholipids. the lipopolysaccharide ...	1985	3968030
immunological and structural relatedness of catabolic ornithine carbamoyltransferases and the anabolic enzymes of enterobacteria.	purified catabolic ornithine carbamoyltransferase of pseudomonas putida and anabolic ornithine carbamoyltransferase (argf product) of escherichia coli k-12 were used to prepare antisera. the two specific antisera gave heterologous cross-reactions of various intensities with bacterial catabolic ornithine carbamoyltransferases formed by pseudomonas and representative organisms of other bacterial genera. the immunological cross-reactivity observed only between the catabolic ornithine carbamoyltrans ...	1985	3968036
identification of hydroxy fatty acids in aeromonas hydrophila, aeromonas sobria, and aeromonas caviae.	gas-liquid chromatographic analysis of fatty acid methyl esters obtained from clinical isolates of aeromonas hydrophila, a. sobria, and a. caviae revealed the presence of two hydroxy fatty acid species, 3-oh 12:0 and 3-oh 14:0.	1985	3934208
in vitro activities of the quinolone antimicrobial agents a-56619 and a-56620.	the in vitro activities of two new quinolone antimicrobial agents, a-56619 and a-56620, were compared with those of norfloxacin, ciprofloxacin, and other antimicrobial agents. the activity of a-56620 was comparable to that of ciprofloxacin against escherichia coli, enterobacter cloacae, and aeromonas hydrophila (mics for 90% of the strains were less than or equal to 0.06 micrograms/ml); acinetobacter anitratus and staphylococcus aureus (mic for 90% of the strains was 0.5 micrograms/ml); and most ...	1985	3935046
[studies of aeromonas spp. isolated from patients with sporadic diarrhea].		1985	3936889
[the "water vibrio" of puntoni in the waters of castellammare di stabia].		1985	3915350
trat gene sequences, serum resistance and pathogenicity-related factors in clinical isolates of escherichia coli and other gram-negative bacteria.	the r6-5 plasmid-specified outer membrane protein, trat protein, has previously been shown to mediate resistance to bacterial killing by serum. colony hybridization with a 700 bp dna fragment carrying most of the trat gene was used to examine the prevalence of trat in gram-negative bacteria, particularly strains of escherichia coli, isolated from clinical specimens. trat was found in isolates of e. coli, salmonella, shigella and klebsiella, but not in pseudomonas, aeromonas or plesiomonas, nor i ...	1985	3900279
[multicenter study of the in vitro effect of imipenem (n-formimidoyl-thienamycin) on hospital bacteria].	minimal inhibitory concentrations (mics) of imipenem were evaluated by agar dilution for 2 895 bacterial strains isolated in 9 hospitals. imipenem proved highly active against enterobacteriaceae, with an mic less than or equal to 0.25 for 63% of the 1 556 tested strains, less than or equal to 1 for 89.6% and less than or equal to 4 for 99%. the different groups of enterobacteriaceae exhibited similar mode mics (0.12 to 0.25), with the exception of serratia (0.25-0.5), p. mirabilis (0.5), indole- ...	1985	3911142
sch 34343: in-vitro antibacterial activity and susceptibility to beta-lactamases.	the in-vitro activity of the penem sch 34343 was assessed in comparison with third-generation cephalosporins, aztreonam and imipenem. sch 34343 was active, with mics generally 2 mg/1 or less, against a broad spectrum of organisms amongst which were staphylococci (including methicillin-resistant strains), streptococci (though enterococci were less susceptible with sch 34343 mics mostly 4-8 mg/1), anaerobes (including the bacteroides fragilis group), haemophilus influenzae and neisseria gonorrhoea ...	1985	3897169
identification of gram-negative bacilli using the autobac idx.	the autobac idx is a new system for the rapid identification of clinically significant members of the enterobacteriaceae and aeromonas, acinetobacter, alcaligenes, flavobacterium, moraxella, and pseudomonas species. the use of 18 differentially inhibitory compounds such as dyes and antibiotics along with a computerized algorithm based on a multivariate analysis provides the basis for the identification of 30 different groups of gram-negative bacilli. required preliminary tests include observatio ...	1985	3881212
etiology and epidemiology of diarrheal diseases in the united states.	accurate data on the frequency of acute diarrheal illness and the distribution of pathogens are not available for several reasons, including the facts that only a small fraction of cases come to the attention of physicians and that available diagnostic tests establish an etiology in only about half of these. in a survey of three groups of patients in a community (upper-middle class and lower class outpatients and hospitalized infants), a possible cause was found in fewer than 20 percent of outpa ...	1985	3893118
gastritis in lake tanganyika cichlids (tropheus duboisii).	necrotic and granulomatous gastritis is described in lake tanganyika cichlids. clostridium hastiforme and flagellated protozoa were both associated with the reaction but the significance of either is unknown. nevertheless, treatment of surviving fish with ampicillin was carried out and mortalities ceased. the possible involvement of an unsuitable diet as a predisposing factor is discussed.	1985	3895712
a comparison of various media in the detection of aeromonas spp. from stool samples.		1985	3896260
loss of virulence in a protease-deficient mutant of aeromonas salmonicida.	the importance of extracellular protease production by aeromonas salmonicida, the bacterial pathogen of fish furunculosis, was investigated with four virulent strains (which were autoagglutinative, hemagglutinative, resistant to fish serum, adhesive to fish tissue culture, protease positive, hemolysin positive, and leukocytolysin positive) and three avirulent strains (which were nonagglutinative, nonhemagglutinative, sensitive to serum, nonadhesive, protease positive, hemolysin positive, and leu ...	1985	3884507
biochemical and exoenzymatic properties of aeromonas species.	one hundred twenty-seven isolates of aeromonas comprising the three currently recognizable species (a. hydrophila, a. sobria, and a. caviae) were evaluated for biochemical and exoenzymatic properties. aeromonas species were generally (greater than 90%) characterized as gram-negative fermentative rods that were oxidase-, catalase-, and beta-galactosidase-positive, produced arginine dihydrolase, and failed to decarboxylate ornithine. more than 95% of all isolates tested failed to grow on 6.5% salt ...	1985	3888508
a comparative study of enterotoxigenic escherichia coli, shigella, aeromonas, and vibrio as etiologies of diarrhea in northeastern thailand.	the incidence of enterotoxigenic escherichia coli (etec), shigella, aeromonas, and vibrio was determined in patients with diarrhea seen at a hospital in northeastern thailand, and compared with the incidence of these bacteria in household contacts and their neighbors. etec was identified in 17%, shigella in 9%, aeromonas in 9%, v. parahaemolyticus in 5%, and non-01 v. cholerae in 2% of 299 patients with diarrhea. these five species of bacteria were isolated more often from patients with diarrhea ...	1985	3890578
yersinia enterocolitica infection in children.	the role of yersinia enterocolitica as a human pathogen has been documented in publications from over 30 countries, and y. enterocolitica has been recognized increasingly to cause gastrointestinal disease in children. in 1979, an australian survey yielded only three isolates of y. enterocolitica from 3298 faecal specimens obtained from adults. we screened all stool specimens received during a 22-month period for yersinia by means of a recently developed selective agar medium. y. enterocolitica w ...	1985	3877859
in-vitro activity of ofloxacin, a quinolone carboxylic acid compared to other quinolones and other antimicrobial agents.	ofloxacin is a new quinolone carboxylic acid compound. its activity against 900 bacterial isolates was determined. it inhibited 90% of escherichia coli, klebsiella sp., aeromonas hydrophila, salmonella spp., shigella spp., citrobacter spp., enterobacter spp., morganella morganii, proteus mirabilis, yersinia enterocolitica at less than or equal to 0.8 mg/l. branhamella catarrhalis, haemophilus sp., neisseria sp. were inhibited by less than or equal to 0.1 mg/l. pseudomonas aeruginosa and other ps ...	1985	3865923
comparative electrophoretic profiles of esterases, and of glutamate, lactate and malate dehydrogenases, from aeromonas hydrophila, a. caviae and a. sobria.	esterases, and glutamate, lactate and malate dehydrogenases of 64 aeromonas hydrophila, a. caviae and a. sobria strains, were analysed by polyacrylamide agarose gel electrophoresis and by thin layer isoelectrofocusing. on the basis of the isoelectric points of malate dehydrogenase from the three species and the mobility of lactate dehydrogenase from a. sobria, 8 species specific zymotypes were defined: three for a. hydrophila strains, three for a. caviae strains and two for a. sobria strains. th ...	1985	3831235
purpura fulminans produced by aeromonas hydrophila: a case report.	a case of purpura fulminans produced by aeromonas hydrophila in a twelve year-old thai boy was reported. the patient also had aplastic anemia which facilitated the severity of disease. he died despite appropriate antimicrobial therapy.	1985	3835694
[gram-negative bacteria resistant to antibiotics in foods].	from 154 food samples, including vegetables (lettuce), milk and meals served at school it was possible to isolate and identify 400 gram negative bacilli distributed among 339 enteric bacteria (escherichia, shigella, citrobacter, klebsiella, enterobacter, serratia and proteus) and other 61 non enteric bacilli (acinetobacter, flavobacterium, aeromonas and pseudomonas). submitting this cultures to the drugs sulfadiazine (su), streptomycin (sm), tetracycline (tc), chloramphenicol (cm), kanamycin (km ...	1985	3837834
molecular cloning and expression of a xylanase gene of alkalophilic aeromonas sp. no. 212 in escherichia coli.	a gene coding for a xylanase activity of alkalophilic aeromonas sp. no. 212 (atcc 31085) was cloned in escherichia coli hb101 with pbr322. plasmid pax1 was isolated from transformants producing xylanase, and the xylanase gene was located in a 6.0 kb hind iii fragment. the pax1-encoded xylanase activity in e. coli hb101 was about 80 times higher than that of xylanase l in alkalophilic aeromonas sp. no. 212. about 40% of the enzyme activity was observed in the periplasmic space of e. coli hb101. t ...	1985	2999298
[aquatic contamination of wounds by aeromonas sobria after bathing in the river].		1985	3160041
nuclease s1 analysis of eubacterial 5s rrna secondary structure.	single-strand-specific nuclease s1 was employed as a structural probe to confirm locations of unpaired nucleotide bases in 5s rrnas purified from prokaryotic species of rrna superfamily i. limited nuclease s1 digests of 3'- and 5'-end-labeled [32p]5s rrnas were electrophoresed in parallel with reference endoribonuclease digests on thin sequencing gels. nuclease s1 primary hydrolysis patterns were comparable for 5s rrnas prepared from all 11 species examined in this study. the locations of base-p ...	1985	3001324
effect of an activated sludge wastewater treatment plant on ambient air densities of aerosols containing bacteria and viruses.	bacteria- and virus-containing aerosols were studied during the late summer and fall seasons in a midwestern suburb of the united states before and during the start-up and operation of an unenclosed activated sludge wastewater treatment plant. the study showed that the air in this suburban area contained low-level densities of indicator microorganisms. after the plant began operating, the densities of total aerobic bacteria-containing particles, standard plate count bacteria, total coliforms, fe ...	1985	2988442
use of non-carbonated soft drinks to provide safe drinking water.	non-carbonated, low-calorie soft drink concentrates (cordials), when diluted according to manufacturers' instructions, had significant antibacterial effects in vitro. bacteria affected include vibrio cholerae, aeromonas hydrophila, shigella sonnei, salmonella typhimurium and escherichia coli. with vibrios, bacterial counts were reduced from 10(6)/ml to undetectable numbers in less than 10 min. escherichia coli in an initial concentration of 10(6)/ml became undetectable after incubation for 1 h w ...	1985	2409896
protein export by a gram-negative bacterium: production of aerolysin by aeromonas hydrophila.	the synthesis and export of aerolysin, an extracellular protein toxin released by the gram-negative bacterium aeromonas hydrophila, was studied by pulse-labeling with [35s]methionine. the toxin was synthesized as a higher-molecular-weight precursor. this was processed cotranslationally, resulting in the appearance within the cell of the mature protein, which was then exported to the supernatant. precursor aerolysin accumulated in cells incubated in the presence of carbonyl cyanide m-chlorophenyl ...	1985	3972770
pathogenicity of aeromonas.	the ability of 15 aeromonas sobria and 9 aeromonas hydrophila isolates to cause subcutaneous lesions was tested. an inoculum of 10(11) colony forming units/l was injected subcutaneously into mice. surviving animals developed a subcutaneous abscess and/or localised skin sloughing and loss of hair (alopecia). an abscess was induced by all nine a. hydrophila isolates and by three of the 15 a. sobria isolates. the induction of local epidermal sloughing and loss of hair followed challenge with either ...	1985	3981022
methods for the detection of haemagglutinins in aeromonas.	when grown in specified conditions and tested by a rocked-tile method, 40 of 41 isolates of two species of aeromonas formed simultaneously at least two haemagglutinins among which were: (i) a mannose-sensitive haemagglutinins with strongest activity for guinea-pig or fowl red cells, formed by all of 31 isolates of a. hydrophila and 9 of 10 isolates of a. punctata ss. caviae; (ii) a haemagglutinin, sensitive to l-fucose or d-mannose, that reacted with human red cells and which was formed by all 4 ...	1985	3981614
holding effects on coliform enumeration in drinking water samples.	standard procedures for analyzing drinking water stress the need to adhere to the time and temperature conditions recommended for holding samples collected for microbiological testing. the national drinking water laboratory certification program requires compliance with these holding limits, but some investigators have reported difficulties in meeting them. research was conducted by standard analytical methods to determine if changes occurred when samples were held at 5 and 22 degrees c and anal ...	1985	4083877
bacteremia caused by aeromonas species in hospitalized cancer patients.	bacteremia caused by aeromonas species occurred in 24 hospitalized patients in a cancer institute during a 13-year period. all but one of these patients had a malignancy (88% had leukemia), and most were receiving chemotherapy for cancer. there was a striking numerical predominance of male patients (82%). unlike some previously described patients with infections due to this organism, none of these 24 patients had recently been exposed to fresh or salt water or to fish. the source of the infectin ...	1985	4023526
clinical and microbiological features of aeromonas hydrophila-associated diarrhea.	the prevalence of aeromonas hydrophila in stool specimens from patients with diarrhea was studied during 18 months. a. hydrophila was found in 1.1% of patients with diarrhea and in none of 533 control patients (p less than 0.02). cases were detected 1.5 times more often during the summer months than the winter months, and most occurred in children less than 2 years of age. clinical features included fever greater than 38 degrees c (55%), abdominal cramps (35%), vomiting (25%), and duration of il ...	1985	4008621