Publications

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in vitro activity of cefixime, a new oral cephalosporin.the in vitro activities of cefixime and seven comparative oral antimicrobials were studied. mic90s of cefixime were 0.015-1 micrograms/ml for haemophilus influenzae, branhamella catarrhalis, escherichia coli, klebsiella, citrobacter diversus, proteus, providencia, aeromonas hydrophila and streptococcus. mic90s for other enterobacteriaceae were greater than or equal to 2 micrograms/ml; 44% of those strains were inhibited by less than or equal 1 microgram/ml. staphylococcus, psuedomonas aeruginosa ...19883219746
seasonal distribution of aeromonas hydrophila in river water and isolation from river fish.the seasonal distribution of aeromonas hydrophila in water and recovery rate from live river fish was investigated. the highest isolation rates of a. hydrophila occurred in water during the late winter followed by a progressive decline in density during the summer and monsoon seasons. the organism was recovered from fish throughout the period from which it was concluded that they form a reservoir which is unrelated to their density in water. the enterotoxigenicity of some environmental strains w ...19883220801
clinical safety and efficacy of ofloxacin.in this study we report about the efficacy and tolerability of ofloxacin in the treatment of 15 patients with severe and moderately severe infections including osteomyelitis (5), soft tissue infections (5), salmonellosis in aids patients (2), acute or chronic pulmonary infections (2) and mediastinitis (1). the following organisms were isolated in culture specimens: staphylococcus aureus (4), pseudomonas aeruginosa (4), staphylococcus epidermidis (3), serratia marcescens (1), escherichia coli (1) ...19883224401
aeromonas infection following a snake bite: a case report.a twelve year-old thai boy with fulminating aeromonas hydrophila infection of the right arm following a snake bite was reported. he recovered uneventfully after appropriate antimicrobial therapy and surgical intervention.19883227407
correlation of the suicide phenomenon in aeromonas species with virulence and enteropathogenicity.certain strains of mesophilic aeromonads (aeromonas hydrophila, a. sorbria, and a. caviae), when grown in broth containing 0.5% glucose, undergo growth inhibition concomitant with acetate accumulation. because these strains are nonviable after 24 h, this phenomenon is termed suicide. we investigated suicidal strains of aeromonas species as a means of understanding animal virulence and enteropathogenicity. to assess virulence, batches of five white mice were inoculated intraperitoneally with 10(7 ...19883230136
preparation of hemolysin from aeromonas. 19883231101
electrophoretic data in designing strategies for purification and identification of a highly polymorphic bacterial esterase.we have purified a bacterial enzyme, designated esterase m, by tailoring an efficient and rapid strategy with information derived from titration curves of proteins in crude extract. the ph-dependent stability of the enzyme activity observed by titration pattern allowed an acidic ph treatment of extract and a cationic exchange chromatography at ph 4.1. these two steps were followed by an anionic exchange chromatography and a preparative electrophoresis. thus, the enzyme was purified about 2000-fo ...19883235767
[septicemia caused by aeromonas/plesiomonas in adults]. 19883244913
[serological cross reactions of lps and lipid a isolated from aeromonas sobria and non 01 vibrio cholerae]. 19883246916
[characteristics of immunoglobulins specific for lps and lipid a of aeromonas sobria and non 01 vibrio cholerae]. 19883246917
[an epidemic of food poisoning by aeromonas hydrophila]. 19883248456
three-dimensional structure of the crystalline surface layer from aeromonas hydrophila.the three-dimensional (3d) structure of the crystalline surface layer of aeromonas hydrophila has been determined to a resolution of 1.3 nm by crystallographic electron microscopy. the s-layer has tetragonal symmetry, with a unit cell dimension of 12.0 nm and a thickness of 5.6 nm. the 3d reconstruction reveals a distinct domain structure, with one main protein mass at one of the fourfold symmetry axes and a minor part at the other.19883249041
aeromonas hydrophila septicaemia and muscle abscesses associated with immunosuppression.we report a case of septicaemia and muscle abscesses due to aeromonas hydrophila. the patient was immunosuppressed due to hodgkin's disease and treatment with cytotoxic chemotherapy. the abscesses resolved after prolonged antibiotic therapy. organisms that were once considered rarely pathogenic are emerging as increasingly important in causing potentially lethal infections in immunosuppressed patients.19883251226
aeromonas hydrophila cellulitis and wound infections caused by waterborne organisms. 19883257483
beta-lactam resistance in aeromonas spp. caused by inducible beta-lactamases active against penicillins, cephalosporins, and carbapenems.use of cefoperazone in a patient with aeromonas caviae in the respiratory tract selected a mutant that constitutively produced beta-lactamase. this mutant, in contrast to its parental strain with an inducible beta-lactamase, showed enhanced resistance to newer cephalosporins and aztreonam. this observation suggested that species of aeromonas, like those of other genera with inducible beta-lactamases, may pose therapeutic problems associated with the rapid development of multiple beta-lactam resi ...19883264129
effect of recent antibacterial agents against bacteria causing diarrhoea.the susceptibility of 424 bacterial isolates causing diarrhoea were tested by agar dilution technique on mueller-hinton agar against amoxicillin, ampicillin, ceftriaxone, chloramphenicol, co-trimoxazole, ciprofloxacin, doxycycline, norfloxacin and ofloxacin. the bacterial species included were aeromonas hydrophila, edwardsiella tarda, pleisomonas shigelloides, salmonella spp., shigella spp., vibrio cholerae and vibrio parahaemolyticus. the most active compounds were the fluorinated 4-quinolones ...19883266900
gene probes for the detection of aerolysin in aeromonas spp. 19883269364
proceedings of the second aeromonas/plesiomonas workshop. miami beach, florida, usa, 7 may 1988. 19883269365
characterization of an aeromonas hydrophila strain isolated on a septicemic out-break in a fish-farm of spain.an aeromonas hydrophila strain (ah-3) was isolated from a septicemic out-break on a gold-fish farm near barcelona (spain). on the bases of their virulence and surface characteristics was classified as moderate to weakly virulent.19883271064
aeromonas hydrophila infections in children. 19883272526
the growth of aeromonas hydrophila k144 in ground pork at 5 degrees c.the influence of nacl, ph, atmosphere, and background microflora on the growth and/or survival of aeromonas hydrophila k144 was studied in ground pork held at 5 degrees c. in ground pork, a hydrophila was sensitive to ph values below 6.0 in the form of either a low starting ph in the pork itself or induced by lactic acid bacteria action on added glucose. growth of the organism is inhibited by nacl levels of 3% (w/w) (approx. 4% brine content). a hydrophila grew in vacuum-packaged ground pork; it ...19883275309
surface protein composition of aeromonas hydrophila strains virulent for fish: identification of a surface array protein.the surface protein composition of members of a serogroup of aeromonas hydrophila which exhibit high virulence for fish was examined. treatment of whole cells of representative strain a. hydrophila tf7 with 0.2 m glycine buffer (ph 4.0) resulted in the release of sheets of a tetragonal surface protein array. sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis analysis showed that this sheet material was composed primarily of a protein of apparent molecular weight 52,000 (52k protein) ...19883276660
tigemonam, an oral monobactam.tigemonam is an orally administered monobactam. at less than or equal to 1 microgram/ml it inhibited the majority of strains of escherichia coli, klebsiella spp., enterobacter aerogenes, citrobacter diversus, proteus spp., providencia spp., aeromonas hydrophila, salmonella spp., shigella spp., serratia marcescens, and yersinia enterocolitica. at less than or equal to 0.25 microgram/ml it inhibited haemophilus spp., neisseria spp., and branhamella catarrhalis. it did not inhibit pseudomonas spp. ...19883279906
molecular cloning of a phospholipid-cholesterol acyltransferase from aeromonas hydrophila. sequence homologies with lecithin-cholesterol acyltransferase and other lipases.we have determined the nucleotide sequence of a gene encoding aeromonas hydrophila phospholipid-cholesterol acyltransferase, an enzyme which shares many properties with mammalian lecithin:cholesterol acyltransferase. the derived amino acid sequence of the protein contains two regions which are homologous to the proposed active sites and binding sites of the plasma acyltransferase and to similar sequences in other interfacially acting lipolytic enzymes. the amino terminus is preceded by a typical ...19883280033
aeromonas hydrophila gas gangrene: a case report of management with surgery and hyperbaric oxygenation. 19883282228
pneumonia due to aeromonas hydrophila-complex: epidemiologic, clinical, and microbiologic features.review of published data examining the various types of infections produced by strains of the aeromonas hydrophila-complex demonstrates a paucity of information on lower respiratory tract infections due to this organism. although it is rarely cited as a cause of pneumonitis, we have been able to collect epidemiologic, clinical, and microbiologic data on eight patients who have had evidence of aeromonas pneumonia. interestingly, seven of the eight patients were male, and the average age for the g ...19883282317
specific inhibition of aspartase by s-2,3-dicarboxyaziridine.aspartase of escherichia coli was inhibited in a competitive manner by s-2,3-dicarboxyazirdine (dcaz), an antibacterial substance against aeromonas salmonesida. the inhibition constant (ki) was 55 microm, which was as low as less than one tenth that of the km value for the substrate, l-aspartate. in view of the fact that both aspartase and fumarase (j. greenhut et al. (1985) j. biol. chem. 260, 6684-6686) were inhibited by dcaz in competitive manners, common features of the reaction mechanism of ...19883289541
partial purification and characterization of an extracellular proteinase from aeromonas hydrophila strain a4.an extracellular metalloproteinase from aeromonas hydrophila strain a4, isolated from milk, was purified by a factor of 300 by chromatography on deae-cellulose and sephadex g-150. the enzyme had a mol. wt of 43,000 and contained 2 g atom ca/mol. it was active over a ph range 4.8-9.5 and had optimum activity on casein at ph 7.0 with km = 0.17 mm. it was strongly inactivated by metal chelators and the apoenzyme was fully reactivated with ca2+, mn2+ or co2+. heavy metal ions such as ag+, hg2+, fe2+ ...19883290294
a comparative study of the formation of extracellular proteins by aeromonas salmonicida at two different temperatures.aeromonas salmonicida was grown in a supplemented 3% (w/v) tryptone soya broth medium at 10 degrees c, a temperature at the lower end of the range over which furunculosis has been observed to occur in the field, and 25 degrees c, the optimum temperature for growth. similar bacterial densities in the range 2.35 +/- 0.05 mg dry wt/ml were achieved in the two cultures at the beginning of the stationary phase of the growth cycle, after 125 h at 10 degrees c and 18 h at 25 degrees c. at this point, a ...19873298187
in vitro activity and beta-lactamase stability of a new monobactam, b0-1165.b0-1165 is a 1-carboxy-1-cyclopropoxyamino,4-fluoromethyl monobactam. it inhibited the majority of escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, citrobacter diversus, aeromonas hydrophila, proteus mirabilis, proteus vulgaris, providencia rettgeri, providencia stuartii, yersinia enterocolitica, haemophilus influenzae, neisseria gonorrhoeae, and salmonella and shigella species at less than or equal to 0.125 microgram/ml. overall, its in vitro activity was similar to that of aztreona ...19873300528
[study of the sensitivity of enteropathogenic bacteria to nifurzide].nifurzide, a synthetic antimicrobial agent of the nitrofuran group, was tested on 320 enteropathogenic bacteria. minimal inhibition concentrations (mic) (expressed in mg/l) were determined using the standard agar dilution technique. the mics were excellent for campylobacter spp. (97 strains), mic 50:0.5, mic 90:0.5; slightly higher for shigella spp. (50 strains) and aeromonas spp. (22 strains), mic 50:2, mic 90:4; and highest for salmonella spp. (100 strains) and yersinia enterocolitica (49 stra ...19873302859
marker exchange mutagenesis of the aerolysin determinant in aeromonas hydrophila demonstrates the role of aerolysin in a. hydrophila-associated systemic infections.we report here on the isolation of isogenic strains of aeromonas hydrophila ab3 deleted for a segment of the aerolysin gene. all aer mutants obtained lacked the 49-kilodalton aerolysin gene product and were neither hemolytic for blood erythrocytes nor cytotoxic for chinese hamster ovary tissue culture cells. one such mutant, ab3-5, was used in a mouse toxicity model to evaluate the role of aerolysin in the pathogenesis of a. hydrophila infections. the strain had a 50% lethal dose (ld50) of great ...19873305370
marine bacteria which produce tetrodotoxin.a number of type strains of marine bacteria, including members of the family vibrionaceae, were cultured and examined for tetrodotoxin productivity by high-performance liquid chromatography and gas chromatography-mass spectrometry. most of the vibrionaceae strains produced tetrodotoxin, anhydrotetrodotoxin, or both.19873310884
natural microbiologic hazards of the aquatic environment. 19873311341
[tte-ras (titertek-enterobac-ras), a new micromethod for the semi-automatic identification of enterobacteriaceae within 5 hours].titertek-enterobac-ras (tte-ras), a new semi-automated system for the identification of the enterobacteriaceae-within five hours, has been evaluated and compared with conventional methods. this note presents the results upon 655 strains, mainly enterobacteriaceae tte-ras provided correct identification for about 92 p. cent and 97 p. cent of enterobacteriaceae respectively before and after carrying out supplementary tests according to manufacturer's instruction. tte-ras gave 97 p. cent specific r ...19873314607
[aeromonas hydrophila as the causative agent of ileocolitis]. 19873315547
[aeromonas spp. as pathogens in human infections: diagnosis and clinical significance].aeromonads have been described as causative agents in a wide variety of infections in immunosuppressed as well as in immunocompetent patients. it is still controversial whether or not some or all three currently recognized species have to be considered as enteropathogenic. clinical and epidemiological data are incomplete and contradictory although laboratory diagnosis does not cause real problems.19873315971
does aeromonas hydrophila preferentially colonize the bowels of patients with hematologic malignancies?weekly cultures of stools from neutropenic patients and bone marrow transplant recipients yielded aeromonas hydrophila from 8% of 88 patients over a 2-yr period. during this time stools in the routine enteric laboratory yielded a. hydrophila in 0.24% of 1632 patients. although the patient groups and culture methods were not directly comparable, this significant difference in isolation rate (p less than 0.001) may reflect a higher colonization rate in the immunocompromised patient.19873319373
inhibition of the aeromonas salmonicida extracellular protease by alpha 2-macroglobulin in the serum of rainbow trout.one of the major pathogenic factors of aeromonas salmonicida, the bacterium causing furunculosis in fish, is considered to be a protease secreted in the extracellular products. this protease can be inhibited by normal trout and rabbit serum but the factor responsible has not hitherto been identified. the present results demonstrate that the a. salmonicida protease is inhibited by the alpha 2-macroglobulin of rainbow trout serum. this inhibitor accounts for about 9% of the total trypsin inhibitin ...19872461505
sodium-channel inhibitors produced by enteropathogenic vibrio cholerae and aeromonas hydrophila. 19872436014
research on aeromonas and plesiomonas. i. taxonomy, ecology, isolation and identification. 19872436939
[accelerated method for differentiating bacteria of the genera vibrio and aeromonas]. 19872437386
serotyping of aeromonas species using passive haemagglutination.antisera were raised with heat-killed vaccines prepared from 25 aeromonas hydrophila strains, 4 a. sobria strains and one a. caviae strain. twenty-seven of these 30 antisera gave high titers when tested in the microtiter tray agglutination (mta) test with their homologous antigen heated at 100 degrees c for 30 min. three o antisera gave low titers in the mta test but reacted to high titers in the haemagglutination (ha) test with conserved sheep red blood cells coated with alkali-treated heat ext ...19872445126
homology of saccharomyces cerevisiae adh4 to an iron-activated alcohol dehydrogenase from zymomonas mobilis.insertion of the transposable element ty at the adh4 locus results in increased levels of a new alcohol dehydrogenase (adh) activity in saccharomyces cerevisiae. the dna sequence of this locus has been determined. it contains a long open reading frame which is not homologous to the other adh isozymes that have been characterized in s. cerevisiae nor does it show obvious homology to drosophila adh. the hypothetical adh does, however, show strong homology to the sequence of an iron-activated adh f ...19872823079
effects of polymyxin b nonapeptide on aeromonas salmonicida.in contrast to polymyxin b-susceptible gram-negative bacteria of human origin, the fish pathogen aeromonas salmonicida was resistant to sensitization by polymyxin b nonapeptide (pmbn) to hydrophobic antibiotics. similarly, sensitization of a. salmonicida strains by pmbn to the bactericidal action of brook trout (salvelinus fontinalis) serum complement was less pronounced than the similar effect of pmbn against other gram-negative bacteria in certain mammalian sera. the surface array protein (a l ...19872823698
molecular analysis of bacterial cytolysins.results of molecular and pathogenic studies of three different bacterial hemolysins (cytolysins) are presented. these exoproteins derive from the two gram-negative bacteria escherichia coli and aeromonas hydrophila and from the gram-positive pathogen listeria monocytogenes. the hemolysin of e. coli is determined by an 8-kilobase (kb) region that includes four clustered genes (hlyc, hlya, hlyb, and hlyd). this hemolysin determinant is part either of large transmissible plasmids or of the chromoso ...19872825323
the effect of different brands of macconkey and xylose lysine deoxycholate agar on the isolation of aeromonas hydrophila.aeromonas hydrophila has been reported to be involved in the opportunistic infection of human beings, therefore, its rapid diagnosis from the clinical specimens was considered to be extremely important for the prognosis. the effect of different brands of macconkey (mac) and xylose lysine deoxycholate (xld) agars on the plating efficiency and colony size of a. hydrophila was evaluated by comparing with blood agar. results indicate that (i) except eiken's mac have low plating efficiency, all the m ...19873428016
enteric pathogens in tropical aquaria.a total of 100 tropical aquaria from six pet shops were sampled for the presence of salmonella, campylobacter and aeromonas species. eight fish tanks contained unusual serotypes of salmonella. none had been used to house terrapins. one hundred and three isolates of aeromonas species were obtained from 98 tanks. one tank contained plesiomonas shigelloides. no campylobacter species were isolated. it is suggested that a tropical aquarium may constitute an infection hazard.19873428370
lymphadenopathy syndrome and hiv infection in multitransfused beta-thalassemia child.lymphoadenopathic syndrome (las), diagnosed also with histologycal studies, is described in a polytransfused 11-year old boy affected by beta-thalassemia major. after three days of the admission he suffered a serious acute diarrhea by aeromonas hydrophila, an opportunistic enteric bacteria demonstrated, up to date, in immunocompromised patients and never in aids patients. it is important to remark that beta-thalassemic patients are at high risk from hiv infections, either for the chronic need of ...19873435653
[spontaneous bacterial peritonitis caused by aeromonas hydrophila. presentation of 2 cases]. 19873438545
two-hour miniaturized system for detection of enteropathogenic bacteria in stool cultures.a miniaturized 2-h system for detection of lactose-negative potentially enteropathogenic bacteria on the basis of nine enzymatic tests was evaluated. the system was challenged with 210 strains of lactose-negative and lactose-positive species grown on macconkey agar. specific and constant patterns were found for salmonella, shigella a-c, yersinia enterocolitica, aeromonas spp. and vibrio cholerae. shigella sonnei and hafnia alvei had an identical pattern, likewise plesiomonas shigelloides, haloph ...19873440458
[geographic variations of the isolation of aeromonas hydrophila in infants with acute infantile diarrhea and a healthy population]. 19873444950
bacterial flora in bottled uncarbonated mineral drinking water.a quantitative study of bacterial populations in mineral water was carried out. samples were stored at 6 and 20 degrees c, and the colony counts were determined on tryptone agar plates incubated at 22 and 37 degrees c. samples were collected from the spring source in sterile glass flasks and from the bottling factory in conventional plastic and glass containers. in both cases, the initial population (10(1)-10(2) cfu/ml water) increased to 10(5)-10(6) cfu/ml after 3 days storage as determined fro ...19873446349
[pulmonary abscess: aeromonas hydrophila caused by a complication of drowning in fresh water]. 19873450216
binding of clavulanic acid to the penicillin-binding proteins of aeromonas hydrophila. 19873509501
in vitro activity of ci-934 compared with ciprofloxacin, enoxacin, norfloxacin, and vancomycin.the in vitro activity of the quinolone ci-934 was compared with ciprofloxacin, norfloxacin, enoxacin, and vancomycin against 607 gram-positive and -negative isolates. ci-934 inhibited 90% of the enterobacteriaceae, aeromonas hydrophila, and acinetobacter spp. at 1 microgram/ml. decreased activity was observed against pseudomonas aeruginosa. pseudomonas maltophilia, and other pseudomonas spp. with ci-934 mic90 greater than or equal to 8 micrograms/ml. ci-934 activity against gram-positive organis ...19873472704
cutaneous infections of the coast. 19873480441
in vitro activity and beta-lactamase stability of a new penem, cgp 31608.the in vitro activity of cgp 31608, a new penem, against aerobic and anaerobic organisms was evaluated and compared with those of other beta-lactams. cgp 31608 inhibited escherichia coli, klebsiella pneumoniae, k. oxytoca, proteus mirabilis, citrobacter diversus, and salmonella, shigella, aeromonas, and yersinia spp. with mics for 50% of the strains (mic50s) of 2 to 4 micrograms/ml and mic90s of 4 micrograms/ml, compared with cefotaxime, ceftazidime, aztreonam, and imipenem mics of less than 0.2 ...19873496845
intestinal infections with aeromonas. 19873544999
identification of major common extracellular proteins secreted by aeromonas salmonicida strains isolated from diseased fish.ten different strains of aeromonas salmonicida that were isolated from diseased fish were grown under identical conditions (24 h at 25 degree c) in 3% (wt/vol) tryptone soya broth medium supplemented with vitamins and inorganic ions. in each case the extracellular proteins that were formed were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and it was found that there were two significant common components, one with a molecular weight of 70,000 and the other with a weight ...19873555336
epidemiological complexity of hospital aeromonas infections revealed by electrophoretic typing of esterases.an epidemiology analysis of a series of 12 aeromonas hydrophila infections, including six of septicaemia, which occurred on several wards of one hospital during the summer of 1982 is presented. the hypothesis that the hospital water could be the source of these infections was supported by the isolation of 1-10 motile aeromonads per ml in most of the water samples collected from various points on the hospital water system. electrophoretic esterase typing was used as an epidemiological screening m ...19873556437
aeromonas cytotonic enterotoxin cross reactive with cholera toxin.isolation by affinity chromatography from crude culture filtrate of aeromonas sobria of protein that cross reacted with cholera toxin (ct) revealed a toxin that produced fluid accumulation in rat ileal loops and in infant mice and caused rounding of y1 adrenal cells. all these activities were neutralised by antiserum to ct. there was no haemolytic or cytotoxic activity associated with this ct-cross reactive cytotonic enterotoxin. ct-cross reactive material detected in enzyme linked immunosorbent ...19873560188
enumeration and characterization of aeromonas hydrophila and aeromonas caviae isolated from grocery store produce.starch-ampicillin agar was used to quantitatively isolate aeromonas sp. from retail grocery store produce. all produce sampled, including parsley, spinach, celery, alfalfa sprouts, broccoli, and lettuce, contained aeromonas sp. in most instances, the count of aeromonas sp. increased 10- to 1,000-fold during 2 weeks of storage at 5 degrees c. eleven (92%) of 12 kinds of produce yielded cytotoxic aeromonas sp. identification as aeromonas hydrophila was the strongest indicator of cytotoxicity, and ...19873566266
[properties of aeromonas hydrophila enterotoxins from a food hygiene viewpoint]. 19873566431
growth of mixed microbial populations in ground water containing highly chlorinated organic wastes. 19873567405
[septicemia caused by aeromonas hydrophila]. 19873567701
isolation frequency of aeromonas species in relation to patient age.over a period of 18 months aeromonas spp. were isolated from stool specimens of 116 patients (total isolation frequency 1.6%). isolation rates in children up to ten years of age were significantly higher only for aeromonas caviae as compared to other age groups. additional data are needed to assess the clinical significance to these findings.19873569253
research on aeromonas and plesiomonas. ii. non-gastrointestinal diseases. 19873569483
research on aeromonas and plesiomonas. iii. enteric infections. 19873569484
catabolism of arginine, citrulline and ornithine by pseudomonas and related bacteria.the distribution of the arginine succinyltransferase pathway was examined in representative strains of pseudomonas and related bacteria able to use arginine as the sole carbon and nitrogen source for growth. the arginine succinyltransferase pathway was induced in arginine-grown cells. the accumulation of succinylornithine following in vivo inhibition of succinylornithine transaminase activity by aminooxyacetic acid showed that this pathway is responsible for the dissimilation of the carbon skele ...19873129535
two-year survey of etiologic agents of diarrheal disease at san lazaro hospital, manila, republic of the philippines.the prevalence of bacterial pathogens and rotavirus in 2,908 patients with diarrhea who were admitted to san lazaro hospital in manila in 1983 and 1984 was determined. one or more enteric pathogens were isolated or detected in samples from 1,698 (58.4%) patients. isolation rates for the various enteropathogens were as follows: rotavirus, 30.6%; shigella spp., 11.6%; salmonella spp., 9.2%; enterotoxigenic escherichia coli (1983 only), 7.8%; vibrio cholerae biotype eltor, 3.8%; non-o1 v. cholerae, ...19873038946
cloning of the gene for the surface array protein of aeromonas salmonicida and evidence linking loss of expression with genetic deletion.a gene bank of dna from the fish pathogenic bacterium aeromonas salmonicida was constructed in the bacteriophage lambda gt11. phage lambda gt11/10g, a recombinant carrying a 4.0-kilobase fragment of a. salmonicida dna, was found to express the surface array protein (a protein) in escherichia coli. sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the protein expressed from the cloned gene had a subunit molecular weight of 49,000, which was identical to that of subunits in the ...19873040676
[fatal aeromonas septicemia in two patients with acute myelogenous leukemia: clinical significance in neutropenic states and review of the literature]. 19873323564
evaluation of a small "conventional" identification system for fermentative gramnegative rods using a computerized data base.a small "conventional" identification system consisting of six tubed media totalling eleven reactions was used to identify 467 strains of enterobacteriaceae, 15 strains of aeromonas sp., and 3 strains of plesiomonas shigelloides. the system identified 371 strains (76.5%) correctly to maximum identification (species or genus) level and an additional 13 strains (2.7%) to genus level where species level identification would have been possible. ninety strains (18.6%) were not identified. only 11 str ...19873326369
phenotypic differentiation of members of the family vibrionaceae using miniaturized biochemical tests.enzymatic hydrolysis of 27 different chromogenic substrates and the assimilation of 44 carbon sources by 144 strains of vibrio species of clinical importance, aeromonas hydrophila and plesiomonas shigelloides were studied by standardized micromethods. some classical biochemical tests were also performed using the test kit tte-as (flow laboratories gmbh, meckenheim, frg). reading of results was done automatically by a photometer and test data were recorded and stored by a microcomputer. all speci ...19873326370
[a case of aeromonas hydrophila sepsis with generalized gas-formation and intravascular hemolysis]. 19873334425
[hospital infections in relation to the use of leeches]. 19872954135
[aeromonas hydrophila infection of the scalp. peculiarities]. 19872955380
construction of a transposon containing a gene for polygalacturonate trans-eliminase from klebsiella oxytoca.a dna fragment containing a klebsiella oxytoca gene for polygalacturonate trans-eliminase was cloned into the kanamycin resistance transposon tn5. this new transposon, designated tn5-pga+, had a transposition frequency of 1 x 10(-6). the broad host range plasmid pr751::tn5-pga+ was conjugally transferred to a variety of genetic backgrounds. the ability to degrade polygalacturonate was expressed in aeromonas hydrophila, alcaligenes eutrophus, azotomonas insolita, escherichia coli, pseudomonas put ...19873034186
purification of cytotoxic enterotoxin of aeromonas sobria by use of monoclonal antibodies.cytotoxic enterotoxin of aeromonas sobria was purified by affinity chromatography with monoclonal antibodies. the purified enterotoxin gave a single protein band in polyacrylamide gradient gel electrophoresis and its mol. wt estimated by this technique was 63,000; it had a pi of 6.2. the purified enterotoxin caused fluid accumulation in rat ileal loops and in infant mice, was cytotoxic to cultured cells, was haemolytic to human erythrocytes, and was lethal to mice after intravenous injection. th ...19873104592
medical leeches as sources of wound infection. 19873107664
in vitro activity of ro 23-6240 (am-833): a new fluoroquinolone.the in vitro activities of ro 23-6240 (am-833) and four comparative fluoroquinolones were studied. minimal inhibitory concentrations (mics) of ro 23-6240, which inhibited at least 90% of strains, were less than or equal to 0.03-1 microgram/ml for staphylococcus aureus, staphylococcus epidermidis, enterobacteriaceae, acinetobacter anitratus aeromonas hydrophila, branhamella catarrhalis, and haemophilus influenzae, 2-8 micrograms/ml for staphylococcus saprophyticus, streptococci, diphtheroids, and ...19873107872
enumeration of pathogenic bacteria from sewage sludge in kuwait.domestic sewage in kuwait is mainly treated by an activated sludge process. sewage sludges at different treatment stages and the digested sludge were studied for their content of pathogenic bacteria. two gram-positive cocci and eight gram-negative bacilli were isolated and enumerated in the sewage sludges and in raw sewage. during march, aeromonas hydrophila was the dominant bacterium isolated. serratia liquefaciens in raw sewage and sludge-2, and salmonella species in digested sludge were domin ...19873121985
are medicinal leeches an infection hazard? 19873153606
post-transplant peritonitis in patients undergoing continuous ambulatory peritoneal dialysis.two patients previously managed by continuous ambulatory peritoneal dialysis for end stage renal failure received cadaveric renal transplants. the peritoneal catheter was capped off and left in situ postoperatively. both patients developed bacterial peritonitis shortly after transplantation. it was felt that the infections were associated with the presence of the indwelling peritoneal catheter as there was no clinical evidence of peritonitis at the time of transplantation.19872886532
[effect of silver preparations on the microflora regenerated from a condensate of atmospheric water vapor in a hermetically-sealed chamber].the effect of different silver compounds (silver sulphate, silver nitrate, electrolytic ionic silver) on microorganisms in water reclaimed from the atmospheric condensate in an enclosed environment was investigated. the following microorganisms were examined: alcaligenes faecalis, citrobacter freundii. aeromonas hydrophila, staphylococcus epidermidis, etc. the ionic silver concentrations in solution varied from 0.1 to 10 mg/l. the efficiency of the silver compounds was found to depend on the mic ...19872886695
the recent evolutionary origin of the phenylalanine-sensitive isozyme of 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase in the enteric lineage of bacteria.evolutionary events that generated the three regulatory isozymes of 3-deoxy-d-arabino-heptulosonate 7-phosphate (dahp) synthase present in contemporary strains of escherichia coli have been proposed recently [ahmad et al. (1986) j bacteriol 165:146-154]. the phylogenetic subdivision of gram-negative prokaryotes studied (superfamily b) includes enteric bacteria, an oceanospirillum cluster, pseudomonad group i (e.g., pseudomonas aeruginosa), pseudomonad group v (e.g., xanthomonas), and the acineto ...19872888901
seasonal prevalence of nosocomial aeromonas hydrophila infection related to aeromonas in hospital water.a seasonal variation in nosocomial aeromonas hydrophila infection was correlated with the number of aeromonas in the hospital water supply. the high summer prevalence of a. hydrophila infection coincided with periods when water counts from storage tanks were highest. the waterborne origin of these infections highlights the importance of maintaining clean water supplies, especially where storage tanks are used. monitoring a. hydrophila in hospital water, particularly during the summer months, may ...19872889768
virulence, cytotoxic and inflammatory activities of vibrio anguillarum and aeromonas salmonicida isolated from cultivated salmonid fish in sweden. 19873687646
comparison of selective media for primary isolation of aeromonas species from human and animal feces.five selective media were evaluated for their effectiveness in the primary isolation of aeromonas spp. from human patients with acute diarrhea and from healthy domestic animals. sheep blood agar with 30 mg of ampicillin per liter (asba30) yielded a significantly higher percentage of positive specimens as compared with the four other media. the effective combination of two selective media with which 98% of all isolates were detected and with which all of the three human-associated aeromonas spp. ...19873693536
clinical significance of aeromonas species isolated from patients with diarrhea.a total of 248 strains of aeromonas spp. were isolated from 3,334 human fecal specimens submitted to a state public health laboratory over a 2-year period to be cultured for enteric pathogens. cary-blair transport medium, blood ampicillin agar, and alkaline peptone water enrichment provided optimal recovery of aeromonas spp. a questionnaire requesting clinical and epidemiological information was sent to physicians, who submitted stool samples for testing, with each laboratory report for 107 cons ...19873693537
evaluation of quantum ii microbiology system for identification of gram-negative bacteria of veterinary origin.the ability of a rapid, semiautomated bacterial identification system, the quantum ii microbiology system (abbott laboratories, irving, tex.), to accurately identify gram-negative bacteria from veterinary sources was evaluated. a total of 378 isolates were tested, including 298 organisms in the family enterobacteriaceae and strains representing acinetobacter sp., aeromonas sp., flavobacterium sp., pasteurella multocida, plesiomonas sp., and pseudomonas spp. of these isolates, 333 (88.1%) were co ...19873693539
isolation of aeromonas sp from faecal specimens. 19873693573
aeromonas hydrophila wound infection associated with myonecrosis and gas gangrene. 19873693881
aeromonas in the gut. 19873693922
adhesion of aeromonas salmonicida strains associated with net electrostatic charges of host tissue cells.the adhesion of aeromonas salmonicida, the pathogenic bacterium of fish furunculosis in salmon and trout, to the surface of host tissue cells was investigated with two fish tissue culture cell lines (rtg-2 cells from rainbow trout, salmo gairdneri, and chse-214 cells from chinook salmon, oncorhynchus tshawytscha) and four a. salmonicida strains. bacterial cells of pathogenic strains were highly adhesive to rtg-2 and chse-214 cells and were negatively charged in the net electrostatic charges, as ...19873818093
interaction of thromboxane a2 and tissue pathology during graded bacteremia.the purpose of this study was to determine whether or not thromboxane a2 (txa2) was necessary or sufficient for the development of end-organ pathology during graded bacteremia. pulmonary artery catheters were placed in 21 adult male pigs under pentobarbital anesthesia and breathing room air. after a control period, animals were studied in four groups: group 1, anesthesia only; group 2, infusion of 1 x 10(9) ml aeromonas hydrophila which was gradually increased from 0.2 ml/kg/hr to 4.0 ml/kg/hr o ...19873820352
aeromonas-associated diarrhea in the netherlands. 19873826975
a modification of bile salts brilliant green agar for isolation of motile aeromonas from foods and environmental specimens.aeromonas has recently been recognized as an important enteropathogen. in faecal specimens the organism is readily identified and differentiated from coliforms by the positive oxidase reaction. however, few media have proved useful for foods and environmental specimens. bile salts brilliant green starch agar (bbgs) was studied for this purpose by testing artificially contaminated foods as well as natural potential sources. the results indicate the suitability of the medium for the isolation of a ...19873595749
activities of aeromonas hydrophila hemolysins and their interaction with erythrocyte membranes.the activity of hemolysin produced by aeromonas hydrophila ca-11, isolated from an environmental source, was more sensitive to temperature than that of hemolysin produced by strain ah-1, isolated from a diarrheal case. ca-11 hemolysin failed to elicit hemolysis below 10 degrees c. immunoblotting analyses showed that both hemolysins formed into oligomers in rabbit erythrocyte membrane even when no hemolysis occurred. suggesting that the binding and the subsequent oligomerization are temperature i ...19873596803
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