| [a comparison of brila-mug and lauryl sulfate-mug bouillon as detection media for total and fecal coliform bacteria in bathing waters in conformity with the economic community guideline 76/160 ewg]. | the spectrum of gram-negative rods occurring in surface waters is wide and depending on the season, i.e. the water temperature. the concentration of bacteria is higher in summer than in winter. however, the seasonal difference of the concentration of enterobacteriaceae is less than that of the aeromonas spec. and of the non-fermenters. the comparison of yields of total and faecal coliforms determined in samples of surface water by using the brilliant green bile lactose 4-methylumbelliferyl-beta- ... | 1993 | 8117394 |
| cloning and nucleotide sequence of an extracellular alpha-amylase gene from aeromonas hydrophila mcc-1. | a gene encoding the extracellular alpha-amylase of aeromonas hydrophila mcc-1 was cloned and expressed using its own promoter on the recombinant plasmid pca101. subcellular fractionation of escherichia coli ja221 carrying pca101 revealed that approximately 60% of the amylase activity was localized in the periplasmic space. the extracellular amylase was purified to homogeneity, identified as an alpha-type and its amino-terminal sequence was determined. nucleotide sequence analysis predicted a 443 ... | 1993 | 8126440 |
| amoxicillin concentrations in the serum of atlantic salmon (salmo salar l) during furunculosis therapy. | the effectiveness of the delivery of amoxicillin to atlantic salmon, undergoing chemotherapy in natural outbreaks of furunculosis in sea-cages, was investigated by measuring the concentration of the drug in serum samples. five groups of 50 sera from three outbreaks were collected two hours after oral treatment with doses of 80 or 120 mg/kg bodyweight. amoxicillin was detected in 82, 82, 92, 100 and 90 per cent of the sera in the five groups (limit of detection 0.16 microgram/ml). many sera conta ... | 1993 | 8128552 |
| antigenicity of the aeromonas salmonicida maltose-inducible outer membrane porin (maltoporin) and a hybrid protein derived from it. | | 1993 | 8131922 |
| bacteriological investigation of the occurrence and antibiotic sensitivities of the gut-flora of the potential southern african medicinal leech, asiaticobdella buntonensis (hirudinidae). | the lack of availability of medicinal leeches is a major impediment to the widespread use of leech therapy for treatment of congested flaps and replants in southern africa. an investigation into the suitability of an alternative leech, the indigenous southern african leech, asiaticobdella buntonensis, was therefore started. the risk of hospital-acquired infection related to the use of leeches and the antibiotic sensitivities of bacteria isolated from the gastro-intestinal tract of wild-caught le ... | 1993 | 8099096 |
| pili and the interaction of aeromonas species with human peripheral blood polymorphonuclear cells. | the interaction of differentially piliated aeromonas strains expressing pili of two broadly different morphologic types (short, rigid (s/r) and/or long, wavy (l/w)) with human peripheral blood mononuclear leukocytes (pmn) was investigated to determine whether host defense cells might exert a selective pressure on pili expression in vivo accounting for the different pili phenotypes of clinical and environmental strains. a majority of aeromonas veronii biotype sobria strains from water (6/6) and f ... | 1993 | 8104626 |
| effects of in vitro nickel exposure on the macrophage-mediated immune functions of rainbow trout (oncorhynchus mykiss). | | 1994 | 8142706 |
| assessment of some selective media for the recovery of aeromonas hydrophila from surface waters. | a comparison was made of three different growing media already proposed by some authors for the recovery of aeromonas hydrophila in environmental water samples of various origins. the media tested were rippey and cabelli m aeromonas agar, rimler-shotts agar and the ryan aeromonas base medium with the addition of ampicillin. the efficiency of the three media was evaluated on the basis of the following reference criteria: accuracy, selectivity and specificity. the results obtained, analyzed by cal ... | 1994 | 8148018 |
| characterisation of haemolytic activity from aeromonas caviae. | aeromonas caviae, an enteropathogen associated with gastroenteritis, displays several virulence characteristics. studies on the kinetics of growth of a. caviae and expression of beta-haemolytic toxin revealed that a. caviae produced maximum haemolytic activity extracellularly during the stationary phase. preliminary studies on the properties of a. caviae haemolysin suggested that divalent cations (mg2+ and ca2+) and thiol compounds, dithiothreitol and mercaptoethanol enhanced the haemolytic acti ... | 1994 | 8150003 |
| mutagenesis of the paracrystalline surface protein array of aeromonas salmonicida by endogenous insertion elements. | the tetragonal paracrystalline surface protein array (a-layer) of the fish pathogenic bacterium aeromonas salmonicida is a virulence factor and bacteria which are unable to produce a-layer are attenuated in their ability to kill fish. ten independent mutants of aeromonas salmonicida which were unable to produce a-layer were isolated by growth at 30 degrees c. these mutants displayed either reduced synthesis of the a-layer subunit, synthesis of a truncated subunit, or complete loss of the ability ... | 1994 | 8151705 |
| enteropathogenic bacteria in river ganges in varanasi. | a bacteriological study of the river ganges in varanasi was carried out from december 1985 to november 1987. in all 407 water samples were collected, 335 from bathing ghats and 72 from sewage openings and were examined for the presence of enteropathogenic bacteria. vibrio cholerae 0-1 (1.72%), non 0-1 vibrio cholera (3.69%), vibrio fluvialis (0.74%), aeromonas sp. (0.49%), plesiomonas sp. (0.25%), salmonella sp. (0.98%) and shigella sp. (1.23%) were isolated from both ghats and sewage openings a ... | 1993 | 8157311 |
| multipoint identification of enterobacteriaceae: report of the british society for microbial technology collaborative study. | to evaluate the accuracy and reproducibility of multipoint identification schemes in a multicentre trial. | 1993 | 8157751 |
| adherence pattern of non-pilated aeromonas hydrophila strains to tissue cultures. | adherence of non-pilated aeromonas hydrophila strains to hep-2, hela, cho and vero tissue culture cells was studied. the strains were isolated from intestinal and extra-intestinal human infections and from the environment. the environmental isolates revealed a diffuse type of adherence to all types of cell lines while clinical isolates showed localized adherence (50%). strains revealing the diffuse type of adherence showed high levels of adhesion i.e. > 20 bacteria per cell. this activity correl ... | 1994 | 8159126 |
| transfer of plasmid rp4 from escherichia coli k-12 to indigenous bacteria of seawater. | transfer of plasmid rp4 from escherichia coli k-12 donor strain to bacteria isolated from seawater was shown to occur on filters and in sterile seawater incubated at 24 degrees c. ten of 12 seawater isolates tested were recipient active for rp4 when the plasmid transfers were assessed by filter matings. when matings were performed in sterile seawater, seven of the 12 isolates received rp4. in sterile seawater, the transfer of rp4 from e. coli to pseudomonads was more efficient than transfer betw ... | 1993 | 8111533 |
| gram-negative bacillary cellulitis in patients with hepatic cirrhosis. | eight episodes of gram-negative bacillary cellulitis in seven patients with hepatic cirrhosis are reported. the patients comprised five women and two men (mean age 59.6 years). the diagnosis was based on a positive culture of specimens obtained by needle aspiration from cutaneous lesions. all patients had grade c cirrhosis according to pugh's classification. cellulitis involved the lower extremities in all cases. five patients developed bullous lesions, three ulcers, two abscesses and two extens ... | 1994 | 8168554 |
| molecular characterization of an enterobacterial metallo beta-lactamase found in a clinical isolate of serratia marcescens that shows imipenem resistance. | a clinical isolate of serratia marcescens (tn9106) produced a metallo beta-lactamase (imp-1) which conferred resistance to imipenem and broad-spectrum beta-lactams. the blaimp gene providing imipenem resistance was cloned and expressed in escherichia coli hb101. the imp-1 was purified from e. coli hb101 that harbors psmbnu24 carrying blaimp, and its apparent molecular mass was calculated to be about 30 kda by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. kinetic studies of imp-1 aga ... | 1994 | 8141584 |
| nucleotide sequence of a novel arylesterase gene from vibro mimicus and characterization of the enzyme expressed in escherichia coli. | a gene coding for an arylesterase of vibrio mimicus was cloned. sequence determination reveals that the esterase gene has an open reading frame of 600 nucleotides which encodes a protein of m(r) 22,300. the deduced amino acid sequence contain a pentapeptide gdsls (residues 27-31), which was also found in the phospholipid-cholesterol acyltransferase from aeromonas hydrophila. substitution of ser-29 by alanine or cysteine in the cloned gene abolished the esterase activity in the tributyrin plate a ... | 1994 | 8141782 |
| antibiotic resistance in clinical and environmental isolates of aeromonas spp. | | 1994 | 8182029 |
| characterization of interfacial catalysis by aeromonas hydrophila lipase/acyltransferase in the highly processive scooting mode. | a glycerophospholipid:cholesterol acyltransferase (gcat) that also has lipase activity is secreted by the bacterium aeromonas hydrophila. hydrolysis of the sn-2-ester bond of 1,2-dimyristoyl-sn-glycero-3-phosphomethanol (dmpm) vesicles by this enzyme is shown to occur in a highly processive scooting mode in which the enzyme, substrate, and the products of hydrolysis remain bound to the vesicle interface. this conclusion is based on the following observations. (a) when there is an excess of vesic ... | 1994 | 8172876 |
| beta-lactam resistance of motile aeromonas isolates from clinical and environmental sources. | the mics of various beta-lactams for 182 isolates of aeromonas species, i.e., a. hydrophila (n = 101), a. sobria (n = 69), and a. caviae (n = 12), from clinical and environmental sources were determined by an agar dilution technique. all strains were resistant to ampicillin and susceptible to aztreonam. a. sobria and a. caviae demonstrated lower resistance rates than a. hydrophila. penicillin-hydrolyzing beta-lactamases were detected in all strains. | 1994 | 8192463 |
| studies on the hemagglutinant activity of some aeromonas strains. | 216 strains to aeromonas genus (158 a. hydrophila, 33 a. salmonicida, 23 a. sobria, 1 a. caviae and 1 a. veronii strains) of different sources of isolation were studied concerning their hemagglutinating behaviour to 5 different erythrocyte species (human, bovine, chicken, african green monkey and guinea pig) in the presence/absence of mannose/fucose. the study aimed to establish the spectrum of their hemagglutinating activity and any possible correlation between the source of isolation, biochemi ... | 1993 | 8173123 |
| [mobile species of the genus aeromonas: difficulties of identification and pathogenicity]. | sixty-two aeromonas strains (39 of clinical and 23 of environmental origin) were identified. the suicide phenomenon and autoagglutination were studied. identification is based on esculin hydrolysis; fermentation of arabinose salicin, sucrose and mannitol; gas production from glucose, indole and beta hemolysis; voges-proskauer and decarboxylation reactions; and finally resistance to cephalothin (30 micrograms) and colistin (4 micrograms/ml). thirty-four per cent of a hydrophila, 33% a caviae, 28% ... | 1993 | 8214815 |
| aeromonas hydrophila infection of a scalp laceration (with synergistic gas-gangrene). | | 1993 | 8216601 |
| distribution of tetracycline resistance determinants among gram-negative bacteria isolated from polluted and unpolluted marine sediments. | tetracycline-resistant gram-negative bacteria were isolated from four different marine sediments in scandinavia and analyzed with dna probes for the determinant classes a to e. colony hybridizations of 429 isolates revealed that class e is the dominating resistance determinant in these marine sediments. comparison of fecally polluted and unpolluted sediments showed few determinant classes in unpolluted sediment and a complex composition of several determinant classes in polluted sediment. total ... | 1994 | 8161183 |
| in vitro activity of ro 23-9424, a dual-acting cephalosporin-quinolone antimicrobial agent. | in vitro activity of new dual-acting antibacterial ro 23-9429 was tested against 1294 bacterial isolates from patients in a major tertiary care referral hospital in saudi arabia. its activity was compared with that of ciprofloxacin, fleroxacin, ampicillin, cephalothin, cefoxitin, cefotaxime, ceftazidime, piperacillin, oxacillin, gentamicin, amikacin, imipenem, and vancomycin. of the 621 members of enterobacteriaceae tested, every single isolate was inhibited by ro 23-9429 at minimum inhibitory c ... | 1993 | 8227462 |
| aeromonas hydrophila peritonitis in a cotton-top tamarin (saguinus oedipus), and retrospective study of infections in seven primate species. | | 1993 | 8231095 |
| an outbreak of enterotoxaemia in suckling camels. | an outbreak of enterotoxaemia was observed for the first time in suckling camels in saudi arabia. the animals were weak, diarrhoeic and succumbed quickly to exertion. the main pathological findings were those of acute catarrhal enteritis and acute myocardial degeneration. clostridium perfringens was isolated from the enteric lesions; aeromonas hydrophila was also identified. the properties of both isolates were studied. | 1993 | 8237187 |
| aeromonas enteropelogenes and aeromonas ichthiosmia are identical to aeromonas trota and aeromonas veronii, respectively, as revealed by small-subunit rrna sequence analysis. | the 16s rrna gene sequences of the type strains of aeromonas enteropelogenes and aeromonas ichthiosmia were determined by polymerase chain reaction direct sequencing in order to clarify their interrelationships with other aeromonad species. on the basis of 16s rrna gene sequence analysis, a. enteropelogenes and a. ichthiosmia were found to be identical to aeromonas trota and aeromonas veronii, respectively. | 1993 | 8240968 |
| biological markers of macrophage activation: applications for fish phagocytes. | the immune defence mechanisms of fish seem to be related and similarly competent to those of mammals. because of this, there is an increased interest in the immune responses of fish as models for higher vertebrates in immunological/immunotoxicological studies. macrophages (m phi), phagocytic cells of the mammalian and teleost immune system which reside in tissues, represent a quiescent population of cells. however, upon stimulation, alterations in the physiology of these resident m phi occur whi ... | 1993 | 8244466 |
| typing of aeromonas spp. by numerical analysis of immunoblotted sds-page gels. | one hundred and three isolates of aeromonas spp., collected from both environmental sources and patients, were examined by sds-page of whole cells followed by immunoblotting with polyclonal rabbit antiserum raised against whole cells of a. sobria. all isolates were typable, yielding 15-20 well separated bands. reproducibility of the technique was good and discrimination excellent, yielding 30 types amongst 103 isolates. immunoblot type was not related to biochemical phenotype. attempts to correl ... | 1993 | 8246248 |
| growth of listeria monocytogenes, aeromonas hydrophila and yersinia enterocolitica in pâté and a comparison with predictive models. | a reference or type strain and a food derived-strain of the cold-tolerant pathogens listeria monocytogenes, aeromonas hydrophila and yersinia enterocolitica were individually inoculated into samples of commercial pâté and incubated at 4 and 10 degrees c. the organisms were periodically estimated by presumptive counts, then values for the lag and generation times were calculated. both strains of l. monocytogenes grew at both temperatures. the food strain of a. hydrophila grew only at 10 degrees c ... | 1993 | 8251302 |
| [microbial enzymes and their inhibitors]. | several proteolytic enzymes and dehydrogenases of microbial origin were studied with special regard to structure-activity relationship. enzyme genes of zn-proteases, subtilisin and pyroglutamyl aminopeptidase from genus bacillus, prolyl endopeptidases from flavobacterium and aeromonas, and of protease ii from e. coli were cloned, sequenced and overproduced in e. coli, their active site structures being elucidated by chemical modification as well as by site-directed mutagenesis. homology analysis ... | 1993 | 8254479 |
| psychrotrophic bacteria in sausage. | a total of 50 samples of frozen fresh beef sausage were collected from grocery stores at beni suef city. these samples were examined for enumeration, isolation and identification of psychrotrophic bacteria. all samples contained psychrotrophic bacteria in variable numbers. the mean counts of psychrotrophic bacteria, psychrotrophic enterobacteriaceae, pseudomonas, aeromonas, brochothrix thermosphacta and lactic acid bacteria were 2 x 10(5) +/- 10(3), 6 x 10(3) +/- 4 x 10(2), 10(3) +/- 2 x 10(2), ... | 1993 | 8259192 |
| adherence of haemagglutinating and non-haemagglutinating clinical and environmental isolates of aeromonas. | twelve haemagglutinating and non-haemagglutinating isolates of aeromonas spp., comprising 6 each of clinical and environmental origin, were examined for their ability to adhere to rabbit intestinal epithelium, for inhibition of adhesion with sugars, and for delineation of the portion of intestine, jejunum, or ileum that is most susceptible to adhesion. although the environmental isolates of aeromonas haemagglutinated human erythrocytes that were inhibited by d-mannose and/or l-fucose, the majori ... | 1993 | 8263304 |
| characterization of mesophilic aeromonas from clinical specimens by computerized analysis of sds-page protein profiles and by enzymatic activity. | mesophilic aeromonas (aeromonas hydrophila, aeromonas sobria, aeromonas caviae) have recently been considered important aetiological agents of human diseases, mainly gastrointestinal infections. although several findings have pointed out the significance of this group of microorganisms as enteric pathogens and suggested the presence of virulence factors, epidemiological and clinical studies are limited by the difficulty of correctly identifying mesophilic aeromonas at the species level. sds-page ... | 1993 | 8264423 |
| oligonucleotide probe determination of tetracycline-resistant bacteria isolated from catfish ponds. | oligonucleotide probes for class a, b and c tetracycline resistance determinants were synthesized and tested individually and in combination for hybridization with gram-negative isolates previously characterized by restriction fragment dna probes. similar hybridization patterns were observed with either probe type. gram-negative catfish pond bacteria were characterized by these oligonucleotide probes. the most common bacteria were plesiomonas shigelloides (47.7%), aeromonas hydrophila (28.8%) an ... | 1993 | 8264667 |
| the spectrum of aeromonas-associated diarrhea in tropical queensland, australia. | during a twelve-month period (1 july 91-30 june 92), feces from 912 persons from the townsville region in the dry tropics of queensland, australia were evaluated by culture and 45 (4.9%) immunocompetent patients were found to be carrying aeromonas in their stools. all patients were index cases and no secondary cases from household or close contact of index cases were identified. eight patients (9.3%) were from palm island, an aboriginal community, and 37 (4.5%) were from the essentially caucasia ... | 1993 | 8266241 |
| distribution of mesophilic aeromonas species in raw and ready-to-eat fish and meat products in switzerland. | a total of 829 poultry, meat, shellfish and fish products commonly consumed in switzerland were qualitatively and quantitatively examined for the presence of mesophilic aeromonas spp. overall, aeromonads occurred in 24.1% of the samples. raw food products were frequently contaminated (e.g. 94.1% in minced meat), with colony counts up to 6.0 x 10(6)/g. some ready-to-eat products had a relatively high percentage of positive samples as well, such as cooked ham in slices (38.2%), mortadella (12.9%), ... | 1993 | 8268055 |
| short-term treatment of traveler's diarrhea with norfloxacin: a double-blind, placebo-controlled study during two seasons. | clinical efficacy of norfloxacin for treatment of traveler's diarrhea in 106 finnish tourists vacationing in morocco was evaluated during two different seasons. when the criteria for diagnosis of traveler's diarrhea were fulfilled, norfloxacin (400 mg) or a placebo was given orally, twice daily for 3 days. all symptoms and signs subsided sooner in the norfloxacin group. the clearest difference was observed in the duration of diarrhea: 1.2 days in the norfloxacin group vs. 3.3 days in the placebo ... | 1993 | 8268363 |
| long term survival of a patient with a regressed giant hepatocellular carcinoma after transcatheter hepatic artery embolization (tae) complicated with liver abscess. | a 62-year-old male patient with histologically proven hepatocellular carcinoma, received transcatheter hepatic artery embolization (tae) therapy. development of right pyothorax and liver abscess at the tumor region occurred 4 months later after tae. aeromonas hydrophila was isolated from the liver abscess. after repeated percutaneous drainage, the abscess cavity disappeared and the tumor became undetectable by ultrasonography. nineteen months after the initial presentation, a second tumor at the ... | 1994 | 8205501 |
| aeromonas hydrophila wound infection associated with water immersion: an unusual football injury. | | 1993 | 8288391 |
| a study of the aetiological agents of childhood diarrhoea in lagos, nigeria. | from december 1989 to may 1990, 315 faecal samples from children under 5 years old with diarrhoea (215) and without diarrhoea (100) seen at paediatric clinics were investigated for bacterial, viral and parasitic enteropathogens. standard and recently described methods were used for the investigations, which revealed that 74.9% of children with diarrhoea were infected with enteropathogens compared with 28% of controls. in the diarrhoeal group, 59.1% had a bacterial, 26.5% a viral and 2.3% a paras ... | 1994 | 8289209 |
| adhesion to and invasion of human colon carcinoma caco-2 cells by aeromonas strains. | the enteropathogenicity of aeromonas strains that showed mannose-resistant adhesion to int407 cells was evaluated by infecting caco-2 cells and observing them by light and electronmicroscopy. five of six strains adhered in large numbers to caco-2 cells in the presence of mannose and caused cytopathic effects. two strains of aeromonas spp. seemed to invade caco-2 cells, as membrane-bound bacteria were seen within the cytoplasm of these cells; however, staining by acridine orange-crystal violet ap ... | 1994 | 8289215 |
| influence of active site and tyrosine modification on the secretion and activity of the aeromonas hydrophila lipase/acyltransferase. | aeromonas sp. secrete a lipase/acyltransferase that shares several properties with the mammalian plasma enzyme lecithin:cholesterol acyltransferase. reaction of the enzyme with tetranitromethane led to modification of 2 tyrosines and a nearly 80% decline in enzyme activity. replacing tyr230 with phe altered the activity of the enzyme in the same way as did treatment with tetranitromethane. unlike the wild type enzyme, which preferentially hydrolyzes the 2-position acyl chain of phosphatidylcholi ... | 1994 | 8294469 |
| physical and chemical characterization of the oligomerization state of the aeromonas hydrophila lipase/acyltransferase. | aeromonas glycerophospholipid:cholesterol acyl transferase undergoes a conformational transition upon activation by treatment with trypsin. chemical cross-linking and sedimentation velocity analysis showed that the lipase dimerizes due to removal of a region near its c-terminus. the lipase monomer has a sedimentation coefficient s20.w = 2.83 s, whereas the dimer has s20.w = 3.65 +/- 0.22 s. hydrodynamic analysis using these sedimentation values and the masses determined by mass spectrometry indi ... | 1993 | 8224197 |
| [comparison of 2 culture media for the isolation of aeromonas spp. from feces]. | | 1993 | 8305566 |
| fate of the fish pathogen aeromonas salmonicida in the peritoneal cavity of rainbow trout. | a model was developed to study the fate of the fish pathogen aeromonas salmonicida in vivo, inside a specialized intraperitoneal chamber implanted in rainbow trout, oncorhynchus mykiss. although normally recalcitrant to lytic agents in vitro, owing to the presence of its regular surface array (s layer), a. salmonicida was rapidly killed in the peritoneal cavity by a host-derived, soluble lytic activity present in peritoneal fluid. peritoneal fluid was also found to kill other bacteria and lyse v ... | 1993 | 8306206 |
| [microbial colonization of the caviar of the sturgeon fishes]. | samples of black caviar in 47.7% of cases are contaminated by opportunistic bacteria aeromonas, proteus, vibrio, v. parahaemolyticus. standardization of these micro-organisms content in black caviar is recommended. | 1993 | 8307402 |
| cloning and characterization of three hemolysin genes from aeromonas salmonicida. | two hemolysin genes (ash3 and ash4) of aeromonas salmonicida strain 17-2 and one (ash1) of a. salmonicida atcc14174 were cloned into the cosmid vector charomid 9-36 in escherichia coli dh1. the overall amino acid sequence of the ash3 was similar to that of the aerolysins of aeromonas hydrophila and aeromonas sobria, and hemolysins ahh3, ahh4, and ahh5 of a. hydrophila, and hemolysin asa1 of a. sobria. the sequence of ash4 was similar to that of the ahh1 hemolysin of a. hydrophila. the ash4 hemol ... | 1993 | 8309354 |
| the role of a 40-megadalton plasmid in the adherence and hemolytic properties of aeromonas hydrophila. | a cured strain of aeromonas hydrophila, ms-2pc, was examined for phenotypic changes in antibiotic resistance, adherence, and hemolysis. parental strain ms-2 was resistant to ampicillin, novobiocin, and carbenicillin; ms-2pc, which lacked a 40-mda plasmid, was also resistant to ampicillin but was sensitive to novobiocin and carbenicillin. the adherence of these isolates to caco-2 and hela cells was examined. ms-2pc demonstrated greater attachment to both cell lines than did strain ms-2 (p < 0.05) ... | 1993 | 8309357 |
| assessment of the antimicrobial sensitivity of aeromonas salmonicida isolates from farmed atlantic salmon in scotland. | sixty-five isolates of aeromonas salmonicida were assessed for antimicrobial sensitivity by disc diffusion tests and the results compared with the minimum inhibitory concentration for each isolate. the results demonstrated that disc diffusion, using a standard technique, may be used to categorize isolates as sensitive or resistant provided that the corresponding minimum inhibitory concentrations are known. | 1993 | 8310605 |
| detection of aeromonas hydrophila serogroup 0:34 in faeces using an enzyme-linked immunosorbent assay. | a microtitration plate, antibody capture, enzyme-linked immunosorbent assay was developed for detection of aeromonas hydrophila serogroup 0:34. the assay uses a detector antibody which shows no cross-reactions with aeromonas strains not belonging to serogroup 0:34 or non-aeromonas competing organisms. the detector antibody is mixed with the sample and incubated for 1 h; it is then microcentrifuged and the supernatant (unabsorbed antibody) titered on a microtiter plate coated with a. hydrophila c ... | 1993 | 8315251 |
| campylobacter-associated diarrhoea in egyptian infants: epidemiology and clinical manifestations of disease and high frequency of concomitant infections. | a diarrhoeal disease survey in alexandria, egypt determined the prevalence, seasonality, and household risk factors for campylobacter-associated diarrhoea in young children. the study population was 880 children (mean age = 9.8 months) presenting with diarrhoea at one of two hospitals. a control group consisted of 1,079 healthy children (mean age = 8.8 months) attending two nearby vaccination clinics. the overall isolation frequencies for campylobacter spp. were 16.8% for cases and 6.4% for the ... | 1993 | 8315255 |
| intestinal flora of the medicinal leech hirudinaria manillensis. | medicinal leeches are widely used to treat venous congestion in microvascular surgery. aeromonas hydrophila infection, following application of the leech species hirudo medicinalis, is a recognized complication. administration of antibiotics directed at aeromonas has been successful in minimizing complications of infection from this organism. a different leech species, hirudinaria manillensis, has recently been introduced for microsurgical use. a study of the enteric content of 30 of these leech ... | 1994 | 8182569 |
| aeromonas sobria spontaneous bacterial peritonitis and bacteremia. | | 1994 | 8304329 |
| fatal septicaemia caused by aeromonas hydrophila in a patient with cirrhosis. | in this case report from italy we describe a fatal infection caused by a. hydrophila in a 39 yr old cirrhotic patient. this pathogen was isolated as a pure single culture from the patient's blood sample. the patient died on the second day of hospitalization from overwhelming sepsis. the a. hydrophila isolate was tested for different potential virulence properties, such as invasiveness, adherence, exotoxins production, presence of fimbriae and for the patterns of resistance to a variety of antimi ... | 1993 | 8281739 |
| [acute gastroenteritis from aeromonas sobria and pancreatitis: apropos of a case]. | | 1993 | 8332027 |
| the antimicrobial activity and beta-lactamase stability of cefpirome, a new fourth-generation cephalosporin in comparison with other agents. | the antimicrobial activity of cefpirome was compared with amoxycillin/clavulanic acid, ampicillin/sulbactam, cefuroxime, ceftazidime, gentamicin and amikacin against 743 non-duplicate clinical isolates. mic50 and mic90 showed that the antibiotic was active against both gram-negative and gram-positive organisms. cefpirome was highly active against most of the enterobacteriaceae, including indole-positive proteus spp., aeromonas spp. (mic < or = 1 mg/l) and salmonella spp. (mic < or = 0.5 mg/l). n ... | 1993 | 8335498 |
| the effect of iron limitation on the growth of aeromonas salmonicida. | the effects of the iron-chelating agents ethylenediamine dihydroxyphenylacetic acid (edda), 2,2'-dipyridyl (dipy) and 8-hydroxyquinoline (8hq) on the growth of aeromonas salmonicida were examined. in these studies, edda caused a small decrease in growth, whereas dipy and 8hq reduced cell growth by 50 and 90%, respectively. the extracellular products contained a greater proportion of the major protease (70 kd) when cells were grown in the presence of the chelating agents. analysis of the outer me ... | 1993 | 8336555 |
| cloning, expression, and sequence analysis of a cytolytic enterotoxin gene from aeromonas hydrophila. | the structural gene and regulatory element for a cytolytic enterotoxin of a diarrheal isolate, ssu, of aeromonas hydrophila was cloned and its dna sequence was determined. a complementary, mixed synthetic oligonucleotide based on the first 10 nh2-terminal amino acid residues of the aeromonas cytolytic enterotoxin was used as a probe to screen a genomic library constructed in bacteriophage embl3. cell lysates of escherichia coli (lambda ch4), containing the cytolytic enterotoxin gene, lysed rabbi ... | 1993 | 8330262 |
| characterisation of strains of aeromonas spp. by phenotype and whole-cell protein fingerprint. | sixty-eight isolates of aeromonas spp. were examined biochemically and their cell proteins were analysed by silver-stained sds-page. protein fingerprints did not correlate with phenotype. however, consideration of both phenotype and fingerprint showed clustering of epidemiologically related isolates. there was also evidence that similar strains could be found in infected people and water or other environmental samples. | 1993 | 8345505 |
| use of tellurite for the selection of verocytotoxigenic escherichia coli o157. | potassium tellurite was assessed for the selection of verocytotoxigenic (vt+) escherichia coli o157. mics were higher for vt+ e. coli o157 than for other strains of e. coli and for aeromonas spp. macconkey medium containing sorbitol, tellurite and cefixime (tc-smac) permitted the growth of vt+ e. coli o157 and shigella sonnei but partially or completely inhibited the growth of 67% of other strains of e. coli and all or most strains of other sorbitol-non-fermenting species tested. of 391 rectal s ... | 1993 | 8345511 |
| metallo-beta-lactamases--a new therapeutic challenge. | | 1993 | 8345513 |
| heat and acid sensitivity of motile aeromonas: a comparison with other food-poisoning bacteria. | the present study was undertaken to compare the heat and acid sensitivity of aeromonads with those of other food-poisoning bacteria. it became obvious that aeromonads were more sensitive to heat than escherichia coli o157:h7, staphylococcus aureus, and salmonella typhimurium. aeromonads were killed in peptone water within 2 min at 55 degrees c, while the other bacteria survived heating at 55 degrees c for more than 15 min. aeromonas cells were also less resistant to heat in hamburger steaks. the ... | 1993 | 8347426 |
| presence of listeria monocytogenes, motile aeromonads and yersinia enterocolitica in environmental samples taken from a supermarket delicatessen. | environmental swab samples collected on three occasions from a supermarket delicatessen were examined for the presence of listeria monocytogenes, motile aeromonads and yersinia enterocolitica. strains of y. enterocolitica belonging to a pathogenic biotype were not recovered, but isolates of l. monocytogenes and motile aeromonads were found. contaminated surfaces and items that came into direct contact with ready-to-eat food included a slicing machine, a knife and parsley that was to be used as a ... | 1993 | 8347431 |
| occurrence of aeromonas spp. in samples of ground meat and chicken. | aeromonas spp. was commonly isolated from ground meat and chicken samples at the retail level. the dominant species in ground meat were a. hydrophila and a. caviae. in chicken, a. sobria was common while a. caviae was isolated infrequently. although a. hydrophila was isolated from 75% of ground meat samples and 62% of chicken samples, dl-lactate-positive a. hydrophila (genospecies 1) was isolated from only 25 or 37% or respective samples. sorbitol-positive a. hydrophila (genospecies 3) was commo ... | 1993 | 8347432 |
| evidence from aeromonas for genetic crossing-over in ribosomal sequences. | | 1993 | 8347521 |
| inhibitory effect against pathogenic and spoilage bacteria of pseudomonas strains isolated from spoiled and fresh fish. | the antibacterial effects of 209 pseudomonas strains isolated from spoiled iced fish and newly caught fish were assessed by screening target organisms in agar diffusion assays. one-third (67 strains) inhibited the growth of one or several of six target organisms (escherichia coli, shewanella putrefaciens, aeromonas sobria, pseudomonas fluorescens, listeria monocytogenes, and staphylococcus aureus), of which s. aureus and a. sobria were the most sensitive. the inhibitory action was most pronounce ... | 1993 | 8357253 |
| host range and transfer efficiency of incompatibility group hi plasmids. | hi plasmids are distinguished by their thermosensitive mode of conjugation (transfer efficiency is optimal at 22-30 degrees c) and their capacity to encode multiple antibiotic resistance. these traits have implicated hi plasmids as potential vectors in the dissemination of antibiotic resistance among pathogenic and indigenous bacterial species in water and soil environments. we compared the transfer efficiency of hi plasmids with that of plasmids from 13 other incompatibility groups at 37, 24, a ... | 1993 | 8358670 |
| molecular cloning and characterization of 13 out genes from erwinia carotovora subspecies carotovora: genes encoding members of a general secretion pathway (gsp) widespread in gram-negative bacteria. | the chemical mutagen ethylmethanesulphonate (ems) has been used to generate mutants of erwinia carotovora subspecies carotovora which are defective in the secretion of pectinases (pel) and cellulases (cel) but unaltered for protease (prt) secretion. such mutants, called out-, still synthesize pel and cel but these enzymes accumulate within the periplasm. cosmid clones carrying wild-type e. carotovora ssp. carotovora dna, identified by their ability to restore the out+ phenotype when transferred ... | 1993 | 8326859 |
| effects of the acetylcholinesterase toxin of aeromonas hydrophila on the central nervous system of fish. | the purified acetylcholinesterase (acche) toxin, crude extracellular products (ecp) or viable virulent aeromonas hydrophila were injected intraperitoneally into rainbow trout in different sublethal and lethal doses. when fish showed signs of morbidity, brain tissue was excised and assayed for acetylcholinesterase activity. in all cases there was a large increase in acche activity (about 40-fold for purified acche-toxin). this was shown to be due to an accumulation of the fish's own acche and not ... | 1993 | 8366818 |
| high specificity of cpha-encoded metallo-beta-lactamase from aeromonas hydrophila ae036 for carbapenems and its contribution to beta-lactam resistance. | the aeromonas hydrophila ae036 chromosome contains a cpha gene encoding a metallo-beta-lactamase highly active against carbapenem antibiotics. this enzyme was induced in strain ae036 to the same extent by both benzylpenicillin and imipenem. when the cpha gene was inserted into plasmid pacyc184, used to transform escherichia coli dh5 alpha, the mics of imipenem, meropenem, and penem hre664 for recombinant clone dh5 alpha(paa20r), expressing the aeromonas metallo-beta-lactamase, were significantly ... | 1993 | 8328781 |
| aeromonas salmonicida grown in vivo. | the virulent fish pathogen aeromonas salmonicida was rapidly killed in vivo when restricted inside a diffusion chamber implanted intraperitoneally in rainbow trout. after a period of regrowth, the survivors had acquired resistance to host-mediated bacteriolysis, phagocytosis, and oxidative killing, properties which were subsequently lost by growth in vitro. resistance to bacteriolysis and phagocytosis was associated with a newly acquired capsular layer revealed by acidic polysaccharide staining ... | 1993 | 8359906 |
| prolyl endopeptidase from aeromonas hydrophila: cloning, sequencing, and expression of the enzyme gene, and characterization of the expressed enzyme. | a strain of aeromonas hydrophila was found to show prolyl endopeptidase activity. the enzyme gene was cloned and expressed in escherichia coli jm83. a 12 kbp ecori fragment containing the enzyme gene was subcloned at the hincii site of puc19 to construct plasmid papep-3 with a 3.5 kbp insert. e. coli jm83 transformed with this plasmid showed about 100-fold higher activity than the parent aeromonas. analysis of the nucleotide sequence of the insert revealed that the mature enzyme-encoding sequenc ... | 1993 | 8370677 |
| [relationship between water pollution and bacterial flora in river water]. | to clarify the relationship between water pollution and bacterial flora in rivers, 132 samples of river water were collected at eleven stations of the chikuma-sai river system from april 1985 to march 1986, and 29 biological-physicochemical examinations for indices of water pollution were done using these samples. species and genus of bacteria in 485 isolates from bacterial flora were identified. although variations of bacterial flora were seen among sampling stations and sampling seasons, most ... | 1993 | 8377255 |
| effect of lactic acid fermentation on bacterial pathogens and indicator organisms in broiler processing waste. | broiler processing offal (heads, viscera, and feet) was collected on 3 separate days from a commercial processing plant. each sample was separately ground, supplemented with sucrose (6% initial concentration), inoculated with actively growing lactic acid bacteria (10(6) cfu/g of offal) from a commercial silage culture, and fermented at 37 c. replicate samples were taken for standard microbiological analysis after 0, 48, and 120 h of fermentation. in fresh offal, heterotrophic plate count, total ... | 1993 | 8378224 |
| failure of the vitek automicrobic system to detect beta-lactam resistance in aeromonas species. | the ability of the vitek automicrobic system (ams; vitek, inc., hazelwood, mo) and disk-diffusion method to detect beta-lactam resistance was assessed with 25 strains from four species of aeromonas. a very major error was indicated when a strain was shown to be susceptible by the ams or disk-diffusion method but resistant by the agar dilution method. the rates for very major errors for disk diffusion and the ams were 0% and 43%, respectively. the beta-lactam agents and numbers of very major erro ... | 1993 | 8379542 |
| [nosocomial aeromonas hydrophila pneumonia complicating toxic coma]. | a 24-year-old male homosexual drug addict was admitted in coma and circulatory failure after a 10 g overdose of acebutolol. the usual resuscitative measures were undertaken, together with administration of adrenaline and gastric lavage. six hours of external cardiac massage and pacing, and high catecholamine doses (36 mg.h-1 of adrenaline and 60 micrograms.kg-1 x min-1 of dobutamine) were required before the circulatory system became again spontaneously efficient. after this acute episode, the p ... | 1993 | 8338270 |
| isolation and purification of aeromonas sobria cytotonic enterotoxin and beta-haemolysin. | aeromonas sp., grown in tryptone soya broth supplemented with yeast extract, 0.6%, ph 7.5, and incubated with agitation at 100 oscillations/min for 15 h at 37 degrees c produced optimal amounts of beta-haemolysin and cytotonic enterotoxin. more prolonged incubation resulted in the loss of enterotoxic activity and anion exchange chromatographic analysis indicated the presence of a moiety capable of breaking down the toxin. anion exchange fast protein liquid chromatography resulted in a single pea ... | 1993 | 8384263 |
| isolation of carbohydrate-reactive outer membrane proteins of aeromonas hydrophila. | outer membrane proteins of aeromonas hydrophila a6 were isolated by affinity chromatography on the basis of their reactivity with trisaccharide structures analogous to the terminal trisaccharide of the h antigen of the human abo(h) blood group system and were characterized by using antisera raised against the isolate. the outer membrane extract for affinity chromatography was prepared from pressure-disrupted outer membranes by differential centrifugation, followed by solubilization of outer memb ... | 1993 | 8380792 |
| purification, gene cloning, amino acid sequence analysis, and expression of an extracellular lipase from an aeromonas hydrophila human isolate. | a structural gene which codes for an extracellular lipase (ec 3.1.1.3) in aeromonas hydrophila h3, which was isolated from a female hospitalized patient, was cloned in escherichia coli by using pbr322 as a vector. lipase purified from both a. hydrophila culture supernatant and the periplasmic fluids of e. coli containing the lip determinant in the original clone (plasmid pla2) showed an m(r) of 67,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, which agrees with the m(r) determ ... | 1993 | 8368830 |
| effects of nutrients on exopolysaccharide production and surface properties of aeromonas salmonicida. | extracellular polysaccharide (eps) and capsular polysaccharide (cps) production by aeromonas salmonicida a450 and the influence of the capsule on cell surface properties were studied. a. salmonicida did not produce cps or eps when glucose, phosphate, magnesium chloride, or trace mineral components were absent from the medium. the addition of yeast extract improved capsule production. neither eps nor cps formation depended on the c/n ratio, although it appeared to be influenced by the level of ca ... | 1993 | 8368833 |
| [clinical and microbiologic characteristics of 282 pediatric patients with mesophilic aeromonas isolated from feces]. | the aim of the study was to assess the microbiological characteristics and the clinical aspects of pediatric patients (age < 14 years) with infectious diarrhea due to one of the species included among the mesophilic aeromonas group (ams) from january 1989 to december 1991. | 1993 | 8399474 |
| molecular cloning and nucleotide sequence analysis of the maltose-inducible porin gene of aeromonas salmonicida. | the gene for the aeromonas salmonicida maltose-inducible porin (maltoporin) was cloned into phagemid ptz18r in two restriction fragments, 0.6-kb psti/kpni and 1.7-kb sphi, of genomic dna and their nucleotide sequences were determined. open reading frames of 1329 and 1335 bp translated into sequences of 443 and 445 amino acids, with a 23 or 25 amino acid signal sequence and a 420 amino acid mature protein of molecular mass 46424 da. putative ribosome binding sites, agga and gggaa, occurred 9 bp u ... | 1993 | 8405961 |
| numerical taxonomy of vibrio & allied organisms. | forty four strains of vibrio, pasteurella, yersinia, aeromonas, escherichia, klebsiella and enterobacter were tested for 44 characters and the overall results scored by the procedure of numerical taxonomy. the strains were clustered by the weighted pair group method with the arithmatic averaging. the v. cholerae 01 and the non-01 v. cholerae being interlinked at a very high level (86% to 97.5%) within a single cluster were considered under the same species. v.cholerae having classic, e1 tor and ... | 1993 | 8406642 |
| novel antigens expressed by aeromonas salmonicida grown in vivo. | virulent and avirulent aeromonas salmonicida strains grown inside intraperitoneal implants in rainbow trout (oncorhynchus mykiss) were examined for unique antigen expression. western blots (immunoblots), performed with immune rabbit serum raised against in vivo-grown cells, revealed several unique antigens. with the exception of lipopolysaccharide (lps), these novel antigens were destroyed after proteinase k treatment. the majority of these antigens were not induced in vitro in response to eithe ... | 1993 | 8406855 |
| isolation and analysis of eight exe genes and their involvement in extracellular protein secretion and outer membrane assembly in aeromonas hydrophila. | the exee gene of aeromonas hydrophila has been shown to be required for the secretion of most if not all of the extracellular proteins produced by this bacterium. in addition, an exee::tn5-751 insertion mutant of a. hydrophila was found to be deficient in the amounts of a number of its major outer membrane proteins (b. jiang and s. p. howard, j. bacteriol. 173:1241-1249, 1991). the exee gene and the exef gene were previously isolated as part of a fragment which complemented this mutant. in this ... | 1993 | 8407845 |
| comparative activity of the new fluoroquinolone rufloxacin (mf 934) against clinical isolates of gram-negative and gram-positive bacteria. | the antibacterial activity of the new fluoroquinolone rufloxacin (mf 934) was evaluated against 1095 clinical isolates and compared with that of other quinolones and various commonly used antibiotics. rufloxacin was highly effective against members of the enterobacteriaceae, inhibiting 98% of the isolates at a concentration of 1 mg/l. ninety-two percent of aeromonas hydrophila and 65% acinetobacter strains tested were inhibited by 1 mg/l of rufloxacin, whereas 98% of methicillin-susceptible and ... | 1993 | 8394816 |
| microbial retention of mercury from waste streams in a laboratory column containing mera gene bacteria. | the microorganisms used for the mercury retention experiments were natural isolates and genetically engineered bacteria. all mercury-resistant strains contained the mera gene. column experiments with these strains were carried out by immobilizing them on different support materials. to obtain kinetic data of the reductase activity for whole cells and the crude extract, batch experiments were carried out under different conditions. | 1993 | 8395193 |
| phylogenetic relationships of marine bacteria, mainly members of the family vibrionaceae, determined on the basis of 16s rrna sequences. | the phylogenetic relationships of 50 reference strains, mostly marine bacteria which require na+ for growth, were determined on the basis of 600 16s rrna nucleotides by using reverse transcriptase sequencing. strains belonging to 10 genera were included (four genera of the family vibrionaceae, the genus aeromonas of the family aeromonadaceae, and the genera alteromonas, marinomonas, shewanella, pseudomonas, and deleya). the sequences were aligned, the similarity values and evolutionary distance ... | 1993 | 8427811 |
| activation of rainbow trout (oncorhynchus mykiss) phagocytes by muramyl dipeptide. | immunoenhancing activity of synthetic muramyl dipeptide (mdp) was investigated in relation to the activation of rainbow trout (oncorhynchus mykiss) phagocytes and to nonspecific protection against challenge with the fish pathogen aeromonas salmonicida. head kidney phagocytes collected from mdp-injected fish showed significant chemotactic activity against zymosan-activated normal rainbow trout serum, and phagocytic activities against both a. salmonicida and plastic beads. the mdp-activated phagoc ... | 1993 | 8388812 |
| a comparison of the amino acid sequence of the serine protease of the fish pathogen aeromonas salmonicida subsp. salmonicida with those of other subtilisin-type enzymes relative to their substrate-binding sites. | the amino acid sequence of the so-called 70 kda (actually 64 kda) serine protease secreted by the gram-negative fish pathogen aeromonas salmonicida has been determined. it shows a high degree of homology with the complete sequence of other bacterial serine proteases which, with molecular masses of approximately 30 kda, are less than half its size. this homology is particularly marked in regions adjacent to the catalytic triad asp32, his64 and ser221 of subtilisin bpn'. significant features of th ... | 1993 | 8436946 |
| gram-negative enteric bacillary meningitis: a twenty-one-year experience. | we reviewed our experience with gram-negative enteric bacillary meningitis in neonates and infants from 1969 through 1989. ninety-eight patients were identified. their ages were from 1 day to 2 years with a median of 10 days. in 25 patients (26%), predisposing factors were identified, the most common of which were neural tube defects and urinary tract anomalies. the causative agents were escherichia coli (53%), klebsiella-enterobacter species (16%), citrobacter diversus (9%), salmonella species ... | 1993 | 8419603 |
| haemagglutinating activity, serum sensitivity and enterotoxigenicity of aeromonas spp. | of 97 isolates of aeromonas spp. that were examined for haemagglutination (ha) and enterotoxigenicity, 35 were from clinical and 62 from environmental sources; 66 of them were also screened for sensitivity to normal human serum (nhs). ha was caused by 44 isolates (45%); it was unrelated to the source of the strain, but it was caused by a higher proportion of the isolates of a. hydrophila than of a. sobria or a. caviae. of the haemagglutinating strains, 82% were enterotoxigenic, whereas most of t ... | 1993 | 8418293 |
| evaluation of commercial presence-absence test kits for detection of total coliforms, escherichia coli, and other indicator bacteria. | evaluations of several commercial presence-absence (p-a) test kits were performed over a 6-month period in 1990 by using the ontario ministry of the environment (moe) p-a test for comparison. the general principles of the multiple-tube fermentation technique formed the basis for conducting the product evaluations. each week, a surface water sample was diluted and inoculated into 25 99-ml dilution blanks for each of three dilutions. the inoculated dilution blanks from each dilution series were ra ... | 1993 | 8434907 |
| why did infection with aeromonas hydrophila occur when water contains so many other microorganisms? | | 1993 | 8448301 |
| aeromonas hydrophila infections of skin and soft tissue: report of 11 cases and review. | we report the clinical and microbiological characteristics of 11 cases of aeromonas hydrophila infection of skin and soft tissue, and we review the english-language literature on such infections. of our 11 patients, seven (64%) presented to the hospital between the months of may and september (inclusive). three patients (27%) had an underlying systemic illness, and two (18%) had nosocomially acquired infection. the nine patients with community-acquired infection had all experienced antecedent tr ... | 1993 | 8448321 |
| a 70 kda aeromonas salmonicida serine protease - beta-galactosidase hybrid protein as an antigen and its protective effect on atlantic salmon (salmo salar l.) against a virulent a. salmonicida challenge. | | 1993 | 8449332 |
| [the pattern of the sensitivity of aeromonas spp. that produce extraintestinal infections]. | we studied the sensibility to several antimicrobics of aeromonas strains isolated in extraintestinal infections during a period of five years, in order to establish which could be the best empirical therapy in these processes. twenty-two strains of a. hydrophila were evaluated, was isolated (8 from hemocultures, 6 from wound exudate, 2 from abscess aspirate, 2 from peritoneal fluid and 1 from urine, pleural fluid, bile and catheter). the identification was made using the gni card system (vitek s ... | 1993 | 8452974 |
| [the amine-forming ability of aeromonas spp]. | motile aeromonas (a.) species are considered international more and more as potential food poisoning organisms. their ability to produce biogenic amines, products of metabolism, which in case can cause a disease, was only searched insufficiently till now. 50 strains of the species a.hydrophila, a.sobria, a.caviae and the non-motile species a.salmonicida were included in the tests for amine producing potency. qualitative investigations to the formation of histamine, tryptamine, and tyramine were ... | 1993 | 8456569 |