Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| identification of nif genes in n2-fixing bacterial strains isolated from rice fields along the yangtze river plain. | the aim of this research was to identify nifh and nifhdkye ' genes in twenty strains of n2-fixing heterotrophic bacteria isolated from rice fields in the yangtze river plain. southern hybridization of the total dna from each strain was performed with the klebsiella pneumoniae nifhdkye ' gene probe (6.2 kb eco ri fragment from psa30) and the azospirillum brasilense nifh gene probe (0.6 kb eco ri-hin diii fragment from phu8). we found that eco ri fragments of total dna from aeromonas hydrophila hy ... | 2006 | 16463319 |
| lack of association between presentation of diarrhoeal symptoms and faecal isolation of aeromonas spp. amongst outpatients in hong kong. | 2006 | 16476802 | |
| cloning and expression analysis of interferon-gamma-inducible-lysosomal thiol reductase gene in large yellow croaker (pseudosciaena crocea). | in mammals, interferon-gamma-inducible-lysosomal thiol reductase (gilt) has been demonstrated to play a key role in the processing and presentation of mhc class ii-restricted antigen (ag) by catalyzing disulfide bond reduction, thus unfolding native protein ag and facilitating subsequent cleavage by proteases. here, we reported the cloning of a gilt gene homologue from the spleen of large yellow croaker, a marine fish (lycgilt). the full-length cdna of lycgilt gene is 1033 nucleotides (nt) encod ... | 2006 | 16478632 |
| sequential binding of cobalt(ii) to metallo-beta-lactamase ccra. | in an effort to probe co(ii) binding to metallo-beta-lactamase ccra, epr, exafs, and (1)h nmr studies were conducted on ccra containing 1 equiv (1-co(ii)-ccra) and 2 equiv (co(ii)co(ii)-ccra) of co(ii). the epr spectra of 1-co(ii)-ccra and co(ii)co(ii)-ccra are distinct and indicate 5/6-coordinate co(ii) ions. the epr spectra also reveal the absence of significant spin-exchange coupling between the co(ii) ions in co(ii)co(ii)-ccra. exafs spectra of 1-co(ii)-ccra suggest 5/6-coordinate co(ii) wit ... | 2006 | 16430228 |
| cell surface display of chi92 on escherichia coli using ice nucleation protein for improved catalytic and antifungal activity. | the gene encoding chitinase 92 (chi92) from aeromonas hydrophila jp10 has been displayed on the cell surface of escherichia coli using the n-terminal region of ice nucleation proteins (inpn) as an anchoring motif. immunofluorescence microscopy confirmed that chi92 was anchored on the cell surface. western blot analysis further identified the synthesis of inp derivatives containing the n-terminal domain inpn-chi92 fusion protein of the expected size (112 kda). whole cell enzyme assay indicated th ... | 2006 | 16487328 |
| cleavage specificity of the serine protease of aeromonas sobria, a member of the kexin family of subtilases. | subtilisin-like proteases have been grouped into six families based on a sequence of the catalytic domain. one of the six is the kexin family, of which furin is a representative protease. all members of the kexin family, except one, are from eukaryotes. the one prokaryotic protease is a serine protease of aeromonas sorbria (asp). here, we examined the substrate specificity of asp based on the cleavage of short peptides. the results showed that asp preferentially cleaves the peptide bond followin ... | 2006 | 16487335 |
| evidence of enhanced bacterial invasion during diplostomum spathaceum infection in european grayling, thymallus thymallus (l.). | farmed grayling, thymallus thymallus (l.), are susceptible to atypical aeromonas salmonicida (aas) infections. interactions between bacteria and parasites were studied using grayling subjected to concomitant exposure to aas bacteria and the digenean parasite diplostomum spathaceum. atypical as was detected from fish by a combination of bacterial cultivation and polymerase chain reaction techniques. a detection level of 17 aas cells per 100 mg intestine tissue sample was obtained. concomitant bac ... | 2006 | 16436118 |
| dietary beta-1,3 glucan potentiates innate immunity and disease resistance of asian catfish, clarias batrachus (l.). | this study investigated the effects of short and prolonged administration of a yeast beta-glucan on non-specific immune parameters, growth rate and the disease resistance of asian catfish, clarias batrachus. fish fed with a basal diet (control) and test diet (basal diet supplemented with 0.1% glucan) for 1, 2 and 3 weeks were assayed for superoxide production, serum myeloperoxidase (mpo) content, natural haemagglutinin level, complement and lysozyme activities. fish were weighed at weekly interv ... | 2006 | 16436120 |
| antibacterial activity of plant extracts from azuki beans (vigna angularis) in vitro. | this study was undertaken to examine the antimicrobial property of azuki beans (vigna angularis). the water extracts of green, black and red colored azuki beans showed antibacterial effects against staphylococcus aureus, aeromonas hydrophila and vibrio parahaemolyticus. in contrast, the extract of white azuki beans showed no inhibition towards any of the microorganisms examined. the extracts of colored azuki beans contained larger amounts of polyphenols including proanthocyanidins than the extra ... | 2006 | 16444673 |
| dna adenine methyltransferase influences the virulence of aeromonas hydrophila. | among the various virulence factors produced by aeromonas hydrophila, a type ii secretion system (t2ss)-secreted cytotoxic enterotoxin (act) and the t3ss are crucial in the pathogenesis of aeromonas-associated infections. our laboratory molecularly characterized both act and the t3ss from a diarrheal isolate, ssu of a. hydrophila, and defined the role of some regulatory genes in modulating the biological effects of act. in this study, we cloned, sequenced, and expressed the dna adenine methyltra ... | 2006 | 16368997 |
| evaluation of the phoenix 100 id/ast system and nid panel for identification of enterobacteriaceae, vibrionaceae, and commonly isolated nonenteric gram-negative bacilli. | the phoenix 100 id/ast system (becton dickinson co., sparks, md.) is an automated system for the identification and antimicrobial susceptibility testing of bacterial isolates. this system with its negative identification (nid) panel was evaluated for its accuracy in the identification of 507 isolates of the family enterobacteriaceae, 57 other nonenteric gram-negative isolates that are commonly isolated in clinical microbiology laboratories, and 138 isolates of the family vibrionaceae. all of the ... | 2006 | 16517878 |
| the udp n-acetylgalactosamine 4-epimerase gene is essential for mesophilic aeromonas hydrophila serotype o34 virulence. | mesophilic aeromonas hydrophila strains of serotype o34 typically express smooth lipopolysaccharide (lps) on their surface. a single mutation in the gene that codes for udp n-acetylgalactosamine 4-epimerase (gne) confers the o(-) phenotype (lps without o-antigen molecules) on a strain in serotypes o18 and o34, but not in serotypes o1 and o2. the gne gene is present in all the mesophilic aeromonas strains tested. no changes were observed for the lps core in a gne mutant from a. hydrophila strain ... | 2006 | 16369010 |
| respiratory burst activity of atlantic cod (gadus morhua l.) blood phagocytes differs markedly from that of rainbow trout. | in the present study we investigated the respiratory burst (rb) activity of atlantic cod (gadus morhua l.) blood phagocytes and we evaluated how the rb activity of cod blood cells differ from that of trout. the rb activities were measured directly from highly diluted whole blood as luminol-amplified chemiluminescence (cl) under various conditions. studies regarding the blood dilutions for cod whole blood chemiluminescence measurements (wbcl) revealed that at a final blood dilution of 1.5 microl ... | 2006 | 16406251 |
| monitoring the zinc affinity of the metallo-beta-lactamase cpha by automated nanoesi-ms. | metallo-beta-lactamases are zinc containing enzymes that are able to hydrolyze and inactivate beta-lactam antibiotics. the subclass b2 enzyme cpha of aeromonas hydrophila is a unique metallo-beta-lactamase because it degrades only carbapenems efficiently and is only active when it has one zinc ion bound. a zinc titration experiment was used to study the zinc affinity of the wild-type and of several mutant cpha enzymes. it shows that a second zn(2+) is also bound at high ion concentrations. all s ... | 2006 | 16406807 |
| immunostimulation of larvae and juveniles of cod, gadus morhua l. | cod larval culture is currently hampered by high mortalities in the first 2-3 weeks after hatching, often due to infectious diseases. the immune system of cod is not fully competent until 2-3 months after hatching. conventional vaccination is, therefore, not of value before this time, and the larvae are wholly reliant on non-specific parameters for their defence against infection. a range of substances, generally derived from bacterial, fungal or plant origin, can activate these non-specific par ... | 2006 | 16533300 |
| dietary administration of bovine lactoferrin influences the immune ability of the giant freshwater prawn macrobrachium rosenbergii (de man) and its resistance against aeromonas hydrophila infection and nitrite stress. | haemocyte count, phenoloxidase activity, agglutinin levels, total protein content, bacterial clearance efficiency, resistance to the pathogen aeromonas hydrophila and nitrite stress were measured in the giant freshwater inter-moult sub-adult prawn macrobrachium rosenbergii (15-20 g) which had been fed diets containing bovine lactoferrin (lf) at 50, 100, 200mg kg(-1) feed for 7 or 14 days. m. rosenbergii fed a diet containing 100mg lf kg(-1) diet for 7 days showed significant (p<0.05) increase in ... | 2006 | 16413208 |
| carriage by the german cockroach (blattella germanica) of multiple-antibiotic-resistant bacteria that are potentially pathogenic to humans, in hospitals and households in tripoli, libya. | using standard bacteriological procedures, 403 cockroaches (blattella germanica) collected in tripoli, from hospitals or the households surrounding the hospitals, were examined for bacteria that are potentially pathogenic to humans. almost all of the cockroaches (96.1% of the 253 from hospitals and 98.7% of the 150 from households) were found to be carrying potentially pathogenic bacteria, with similar mean burdens of 3.2 x 10(5) colony-forming units (cfu) (range=0-1.4 x 10(7)) for each hospital ... | 2006 | 16417714 |
| necrotizing fasciitis caused by aeromonas hydrophilia in an immunocompetent child. | necrotizing fasciitis is a rapidly progressive and potentially fatal infection. it is characterized by extensive subcutaneous and muscle necrosis. aeromonus hydrophilia is a gram-negative faculatively anaerobic bacillus that is part of the normal flora of nonfecal sewage and is found in most water systems, soil, and fresh brackish water. necrotizing fasciitis caused by a. hydrophilia have occurred in children who had underlying systemic diseases or immune dysfunction. we report a 14-year-old boy ... | 2006 | 16418613 |
| spontaneous bilateral bacterial empyema in a patient with nephrotic syndrome. | spontaneous bacterial empyema (sbem), a rare infectious complication among liver cirrhosis patients, is characterized by infection in the presence of pleural effusion without evidence of pre-existing pneumonia. the prevalence of sbem in cirrhotic patients with hydrothorax is about 13%. however, it has previously not been reported in medical literature in patients with nephrotic syndrome. the most common microorganism identified is escherichia coli, followed by streptococcus species, enterococcus ... | 2006 | 16457891 |
| [beta-lactamases of gram negative bacteria: never-ending clockwork!]. | the acquired resistance against the wide-spectrum and highly stable beta-lactams including third-generation cephalosporins (3gc) and carbapenems is constinuously increasing and widespead with the discovery of various plasmid-encoded, or genes cassette or integrons coding for a novel beta-lactamase, always a major mechanism of resistance. to explain resistance against 3gc, with the continuing story with tem and shv mutated enzymes, several types of esbl (class a) emerge the ctx-m type, at least c ... | 2006 | 16420989 |
| identification and characterization of an aeromonas salmonicida (syn haemophilus piscium) strain that reduces selenite to elemental red selenium. | a bacterium that reduces toxic and mobile selenite to insoluble elemental selenium (se0) was isolated from a laboratory scale permeable reactive biobarrier. biochemical tests and 16s rrna gene sequence alignment identified the isolate as aeromonas salmonicida. two colony types were isolated, one more resistant to selenite than the other. both grew on agar plates containing 16 mm: selenite, although the colony diameter was reduced to 8% of controls with the small colony type and to 18% with the l ... | 2006 | 16550462 |
| potential involvement of galectin-3 and snap23 in aeromonas hydrophila cytotoxic enterotoxin-induced host cell apoptosis. | we investigated the potential of the cytotoxic enterotoxin (act) of aeromonas hydrophila to bind to 1869 human and 4319 yeast proteins, using protein microarray technology. act was capable of binding nine different human proteins, including the snare complex scaffolding protein synaptosomal-associated protein 23 (snap23), galectin-3, and guanylate kinase 1 (guk-1). act was also able to bind to four of the yeast proteins examined, which included the vesicle tethering protein vsp52. we verified in ... | 2006 | 16426811 |
| detection of the frequency, antimicrobial susceptibility, and genotypic discrimination of aeromonas strains isolated from municipally treated tap water samples by cultivation and ap-pcr. | the frequency, antibiotic susceptibility, and genotypic discrimination of aeromonas strains isolated from municipally treated drinking tap water distribution systems were investigated in this study. we have analyzed 148 tap water samples collected from 8 different locations by bacterial cultivation and arbitrarily primed polymerase chain reaction (ap-pcr). gram negative, hemolytic, oxidase (+) and catalase (+) bacterial colonies were applied to the study. identification of bacterial colonies was ... | 2006 | 16427154 |
| analysis of the lateral flagellar gene system of aeromonas hydrophila ah-3. | mesophilic aeromonas strains express a polar flagellum in all culture conditions, and certain strains produce lateral flagella on semisolid media or on surfaces. although aeromonas lateral flagella have been described as a colonization factor, little is known about their organization and expression. here we characterized the complete lateral flagellar gene cluster of aeromonas hydrophila ah-3 containing 38 genes, 9 of which (lafa-u) have been reported previously. among the flgll and lafa structu ... | 2006 | 16428388 |
| metabolic engineering of aeromonas hydrophila for the enhanced production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate). | wild-type aeromonas hydrophila 4ak4 produced 35-45 wt.% poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (phbhhx) consisting of 10-15 mol% 3-hydroxyhexanoate (3hhx). to enhance phbhhx production, vgb gene encoding vitreoscilla haemoglobin or fadd gene encoding escherichia coli acyl-coa synthase was co-expressed with polyhydroxyalkanoates (pha) synthesis-related genes including phbab from wautersia eutropha and phapcj from a. hydrophila. expression of vgb increased phbhhx content from 46 to 53 wt.% ... | 2006 | 15983806 |
| effect of achyranthes aspera on the immunity and survival of labeo rohita infected with aeromonas hydrophila. | achyranthes aspera seed was incorporated in the diets (at 0.01%, 0.1% and 0.5%) of labeo rohita, rohu fingerlings (3.0+/-0.4 g). after 2 weeks, the fish were immunized with heat-killed aeromonas hydrophila, and after a further 2 weeks the rohu were experimentally infected with aeromonas hydrophila (atcc 49140). after 7 days blood and serum were sampled to determine superoxide anion production, bactericidal activity, lysozyme, serum protein, albumin, globulin, serum glutamate oxaloacetate transam ... | 2006 | 15961319 |
| plasma proteomic analysis of the acute phase response of rainbow trout (oncorhynchus mykiss) to intraperitoneal inflammation and lps injection. | few acute phase proteins are known in fish and better knowledge of them would provide a basis for more reliable methods to objectively assess fish health and welfare. an acute phase response was induced in rainbow trout (oncorhynchus mykiss, walbaum) by inflammation triggered by intraperitoneal administration of purified aeromonas salmonicida lipopolysaccharide emulsified in freund's incomplete adjuvant (lps/fia) or a commercial oil-based multivalent vaccine. acute phase proteins were characteri ... | 2006 | 16139357 |
| identification of a transcript encoding a soluble form of toll-like receptor 5 (tlr5) in atlantic salmon during aeromonas salmonicida infection. | toll-like receptors (tlrs) are involved in the innate immune response against microbial pathogens in vertebrates and insects. the extracellular region of a tlr recognizes pathogen-associated molecules, while the intracellular region initiates the signaling pathway leading to immune response. membrane-bound tlrs have been found in most vertebrates, but few soluble forms have been reported. a novel transcript corresponding to a portion of a soluble tlr was identified in liver of infected atlantic ... | 2006 | 16112748 |
| passive transfer of maternal antibodies and their existence in eggs, larvae and fry of indian major carp, labeo rohita (ham.). | lack of immune competence in the early stages of life leads to severe mortality in larval stages of different fish species including indian major carp (imc). investigation through indirect enzyme linked immunosorbent assay (elisa) and agglutination test revealed a significant increase in specific serum antibody response in the brood fish of indian major carp, labeo rohita (ham.) following immunisation with a virulent aeromonas hydrophila bacterin 1 month prior to breeding, which was transferred ... | 2006 | 16157486 |
| purification and characterization of alpha2-macroglobulin from grass carp ctenopharyngodon idellus: cloning a segment of the corresponding gene. | the plasma protein alpha2-macroglobulin (alpha2m) was purified by gel filtration and anion-exchange chromatography from grass carp plasma. the alpha2m consists of two different subunits of molecular weight 95 kda and 80 kda, respectively. the characteristics of grass carp alpha2m are similar to mammalian alpha2m, in that grass carp alpha2m exists in two forms: a fast-form and a slow-form. the former is complexed with protease. the sequence of grass carp alpha2m-conserved region and a region cont ... | 2006 | 16139520 |
| study on peptide hydrolysis by aminopeptidases from streptomyces griseus, streptomyces septatus and aeromonas proteolytica. | we developed a spectrophotometric assay for peptide hydrolysis by aminopeptidases (aps). the assay enables the measurement of free amino acids liberated by ap-catalyzed peptide hydrolysis using 4-aminoantipyrine, phenol, peroxidase, and l-amino acid oxidase. we investigated the specificity of bacterial aps [enzymes from streptomyces griseus (sgap), streptomyces septatus (ssap), and aeromonas proteolytica (aap)] toward peptide substrates using this assay method. although these enzymes most effici ... | 2006 | 16080009 |
| effect of multiple injections of beta-glucan on non-specific immune response and disease resistance in labeo rohita fingerlings. | the purpose of this study was to determine if multiple injections of different dosages of beta-glucan derived from barley would enhance the immune response and disease resistance against infections due to opportunistic pathogens aeromonas hydrophila and edwardsiella tarda in labeo rohita fingerlings. hence, four different dosages of beta-glucan suspension in phosphate-buffered saline at the rate of 0, 5, 10, 15 mg kg(-1) body weight of fish were injected intraperitoneally to the fingerlings of l ... | 2006 | 16039142 |
| the development of a matrix-assisted laser desorption/ionization mass spectrometry-based method for the protein fingerprinting and identification of aeromonas species using whole cells. | this report describes the development of a method to detect the waterborne pathogen aeromonas using matrix-assisted laser desorption/ionization mass spectrometry (maldi-ms). the genus aeromonas is one of several medically significant genera that have gained prominence due to their evolving taxonomy and controversial role in human diseases. in this study, maldi-ms was applied to the characterization of seventeen species of aeromonas. these seventeen species were represented by thirty-two strains, ... | 2006 | 16176841 |
| aeromonas septicaemia: an uncommon complication following placement of transhepatic biliary drainage devices in europe. | 2006 | 16099547 | |
| the contribution of aeromonas salmonicida extracellular products to the induction of inflammation in atlantic salmon (salmo salar l.) following vaccination with oil-based vaccines. | intraperitoneal injection of atlantic salmon with oil-based vaccines often results in severe side effects. aeromonas salmonicida subspecies salmonicida, a primary antigen in the vaccines, produces extracellular products (ecps) that are included in the formulation but the role of ecps in inducing side effects is not well understood. in the present study, we evaluated the contribution of ecps to early inflammatory reactions since early events determine the outcome of inflammation. five groups of a ... | 2006 | 16018934 |
| experimental handling stress as infection-facilitating factor for the goldfish ulcerative disease. | experimental handling stress (ehs) was applied to clinically asymptomatic farmed goldfish (carassius auratus l.). ehs affected the gills and skin integrity of the fish and was accompanied by increased levels of plasma glucose, cortisol and interleukin-10 (il-10). ehs application was followed by highly significant enhancement of the rate of infection with a virulent aeromonas salmonicida isolate. cumulative ulceration at the initial phase of the ensuing goldfish ulcerative disease (gud) evidenced ... | 2006 | 16174536 |
| comparison of elisa and pcr vis-à-vis cultural methods for detecting aeromonas spp. in foods of animal origin. | the present study was conducted to assess the best method of the most commonly used methods for detection of aeromonads in foods of animal origin. with this objective an omp based indirect plate elisa and a duplex-pcr using primers targeting aerolysin gene and 16s rrna gene and yielding amplicons of 252 bp and 599 bp, respectively, were standardized. the standardized protocols and the conventional cultural method were then compared for their respective sensitivities and specificities for detecti ... | 2006 | 16216375 |
| influence of high-m alginate on the growth and survival of atlantic cod (gadus morhua l.) and spotted wolffish (anarhichas minor olafsen) fry. | atlantic cod and spotted wolffish fry were fed high-m alginate containing feed for 59 and 55 days, respectively. during this period the fry showed a higher specific growth rate compared to controls. uptake and distribution of alginate was studied by inclusion of the (125)i-labelled molecule in the feed. the stomach and intestine contained the highest amount while the kidney, liver and spleen contained some, indicating that the alginate was taken up by the gut and transported to internal organs. ... | 2006 | 16219475 |
| a comparative immunopathological study of injection site reactions in salmonids following intraperitoneal injection with oil-adjuvanted vaccines. | an adequate understanding of the inflammatory mechanisms following intraperitoneally-injected oil-adjuvanted vaccines in fish is required for the improvement of the quality of vaccines. we report the findings of a study in which the importance of neutrophils, macrophages, lymphocytes and mast cells (mcs) in early inflammatory reactions of atlantic salmon were examined in comparison with those of rainbow trout and grayling. the inflamed areas in the pylorus/pancreas area (injection site) were seq ... | 2006 | 16221512 |
| structural studies of the core region of aeromonas salmonicida subsp. salmonicida lipopolysaccharide. | the core oligosaccharide structure of the in vivo derived rough phenotype of aeromonas salmonicida subsp. salmonicida was investigated by a combination of compositional, methylation, ce-ms and one- and two-dimensional nmr analyses and established as the following: [carbohydrate: see text] where r=alpha-d-galp-(1-->4)-beta-d-galpnac-(1--> or alpha-d-galp-(1--> (approx. ratio 4:3). comparative ce-ms analysis of a. salmonicida subsp. salmonicida core oligosaccharides from strains a449, 80204-1 and ... | 2006 | 16297894 |
| transcriptome response following administration of a live bacterial vaccine in atlantic salmon (salmo salar). | antibacterial responses have been studied in atlantic salmon following an acute intra peritoneal injection of a genetically attenuated (aroa(-)) strain of aeromonas salmonicida known to elicit protective immunity. three tissues were studied for transcriptional changes, the liver, head kidney and the gill. rna was collected from fish 6, 12, 24 and 48 h following infection or at the same time points from fish injected with pbs as non-infected control. pcr-select cdna subtraction libraries were con ... | 2006 | 16313960 |
| immunostimulatory nature of beta-glucans and baker's yeast in gnotobiotic artemia challenge tests. | the use of new preventive approaches such as immunostimulants to reduce stress and mortalities, to maintain good health of cultured organisms and to stimulate the non-specific defence mechanism, is becoming increasingly important in aquaculture. yet detailed analysis reveals that in most experiments the validity of some conclusions with respect to the benefit of immunostimulation is still doubtful, especially in invertebrates. the use of standardized trials under controlled rearing conditions, c ... | 2006 | 16257233 |
| synthesis and evaluation of new quinazolone derivatives of nalidixic acid as potential antibacterial and antifungal agents. | in continuation of our work on synthesis of biheterocycles carrying the biodynamic heterocyclic systems at position 3, a series of new nalidixic acid derivatives having quinazolones moiety were synthesised to achieve enhanced biological activity and wide spectrum of activity. nalidixic acid was first converted into its acid chloride using thionyl chloride as an acylating agent at laboratory temperature. later it was converted to methyl ester. nalidixoyl chloride formed vigorously reacts with met ... | 2006 | 16260068 |
| modulation of juvenile brook trout (salvelinus fontinalis) cellular immune system after aeromonas salmonicida challenge. | in fish, the first line of defence against infectious microorganisms is based on non-specific cellular immune mechanisms (innate immunity). in this study, we measured the non-specific immune parameters (natural cytotoxic cells (ncc) activity, lymphoproliferation, percentage of phagocytosis and phagocytic activity) in brook trout (salvelinus fontinalis) infected by a virulent strain of aeromonas salmonicida. eight days post-infection, the mortality of infected fish reached 70%. a transient immuno ... | 2006 | 16263179 |
| immunoreactive antigens of the outer membrane protein of aeromonas hydrophila, isolated from goldfish, carassius auratus (linn.). | aeromonas hydrophila causes disease under stress conditions or in concert with infection by other pathogens in goldfish. sero-diagnostic and/or immunoprophylactic tools against aeromonas infection in goldfish are not available so far. the present study was undertaken to fractionate and characterise the outer membrane proteins (omp) of a. hydrophila and to identify suitable immunoreactive components. a total of 10 fractions were generated from crude omp antigens upon gel permeation and subsequent ... | 2006 | 16243540 |
| microbiological evaluation of bottled non-carbonated ("still") water from domestic brands in greece. | the microbiological quality of 1,527 samples of bottled non-carbonated ('still') mineral water, purchased from retail outlets and derived from 10 manufacturing companies in greece, was investigated during the period 1995-2003. applying the membrane filter technique, the aliquots of water samples (250 ml) were analyzed for the presence and enumeration of total coliforms, escherichia coli, enterococcus spp. and pseudomonas aeruginosa. also, aerobic bacteria were counted as heterotrophic plate coun ... | 2006 | 16271413 |
| effect of sex ratio on the immune system of oreochromis mossambicus (peters). | the social environment of fish has a crucial role to play on the immune system and hence on the overall health status. stressors of social origin such as dominance, subordination, and fight for mate have a major impact on the immune system of fish. the present study was designed with the objective of finding the effect of sex ratio of the population on the immune system of oreochromis mossambicus. groups of fish were maintained for 28 days in three different sex ratios i.e., (i) all-male (ii) al ... | 2006 | 16256305 |
| the immunomodulatory effects of tuftsin on the non-specific immune system of indian major carp, labeo rohita. | the purpose of this study was to determine if injections of different dosages of tuftsin would enhance the immune response and disease resistance against the infections due to the opportunistic pathogens aeromonas hydrophila and edwardsiella tarda in labeo rohita fingerlings. hence, four different dosages of tuftsin in pbs suspension at the rate of 0, 5, 10, 15 mg kg(-1) body weight of fish were injected intraperitoneally to the fingerlings of l. rohita at 2-week intervals for four times. after ... | 2006 | 16293422 |
| adjuvant effect of propolis on immunisation by inactivated aeromonas hydrophila in carp (carassius auratus gibelio). | 2006 | 16325428 | |
| macrolide inactivation gene cluster mpha-mrx-mphr adjacent to a class 1 integron in aeromonas hydrophila isolated from a diarrhoeic pig in oklahoma. | to characterize a multidrug-resistant aeromonas hydrophila isolate (cvm861) that possesses a high-level macrolide inactivation gene cluster (mpha-mrx-mphr), previously only reported in escherichia coli. | 2006 | 16339607 |
| effects of linear cationic alpha-helical antimicrobial peptides on immune-relevant genes in trout macrophages. | there are increasing evidence of the potential role of antimicrobial peptides in the regulation of immune responses in mammalian species. however, the effects of these peptides in fish have yet to be investigated. in this study, we examined the transcriptional expression profile of representative immune-relevant genes in a trout macrophage cell line, rts11, in response to three linear cationic alpha-helical antimicrobial peptides (insect cecropin b, fish pleurocidin and a cecropin analogue cf17) ... | 2006 | 16352337 |
| polar flagellum biogenesis in aeromonas hydrophila. | mesophilic aeromonas spp. constitutively express a single polar flagellum that helps the bacteria move to more favorable environments and is an important virulence and colonization factor. certain strains can also produce multiple lateral flagella in semisolid media or over surfaces. we have previously reported 16 genes (flgn to flgl) that constitute region 1 of the aeromonas hydrophila ah-3 polar flagellum biogenesis gene clusters. we identified 39 new polar flagellum genes distributed in four ... | 2006 | 16385045 |
| interactions between peptidoglycan and the exeab complex during assembly of the type ii secretin of aeromonas hydrophila. | aeromonas hydrophila transports extracellular protein toxins via the type ii secretion system, an export mechanism comprised of numerous proteins that spans both the inner and outer membranes. two components of this secretion system, exea and exeb, form a complex in the inner membrane that functions to locate and/or assemble the exed secretin in the outer membrane. in the studies reported here, two-codon insertion mutagenesis of exea revealed that an insertion at amino acid 495 in the c-terminal ... | 2006 | 16420372 |
| catalytic mechanism of class b2 metallo-beta-lactamase. | the initial nucleophilic substitution step of biapenem hydrolysis catalyzed by a subclass b2 metallo-beta-lactamase (cpha from aeromonas hydrophila) is investigated using hybrid quantum mechanical/molecular mechanical methods and density functional theory. we focused on a recently proposed catalytic mechanism that involves a non-metal-binding water nucleophile in the active site of the monozinc cpha. both theoretical models identified a single transition state featuring nearly concomitant nucleo ... | 2006 | 16423823 |
| aeromonas veronii biovar sobria bacteraemia with septic arthritis confirmed by 16s rdna pcr in an immunocompetent adult. | an 81-year-old man developed septic arthritis and bacteraemia with aeromonas veronii biovar sobria. cultures of the joint wash-out fluid were negative; however, dna matching that of aeromonas veronii was identified by 16s rdna pcr. the patient was successfully treated with a 4 week course of ciprofloxacin. no recognized risk factors were found. | 2006 | 16434719 |
| [cloacal and nasal bacterial flora of lepidochelys olivacea (testudines: cheloniidae) from the north pacific coast of costa rica]. | cloacal and nasal bacterial flora of lepidochelys olivacea (testudines: cheloniidae) from the north pacific coast of costa rica. the aerobic cloacal and nasal bacterial flora of 45 apparently healthy female olive ridley sea turtles (lepidochelys olivacea) was studied at nancite nesting beach, in santa rosa national park (costa rican north pacific) during july and august 2002. bacterial samples were obtained by inserting sterile swabs directly into the cloaca and the nasal cavities of the turtles ... | 2006 | 18457173 |
| enteropathogenic bacteria and enterotoxin-producing staphylococcus aureus isolated from ready-to-eat foods in khon kaen, thailand. | the objective of this study was to investigate the microbiological quality of ready-to-eat food in the municipality of khon kaen, thailand. four categories of 186 food samples were collected: (1) high heat food; (2) low heat food; (3) no heat food; and, 4) on-site prepared fruit juices and beverages. of the food samples, 145 (78%) failed to meet acceptable microbiological standards, including fruit juice and beverages (100%), no heat food (91.7%), low heat food (81.7%) and high heat food (57.9%) ... | 2006 | 17333744 |
| detection of conjugation related type four secretion machinery in aeromonas culicicola. | background: aeromonas sp. can now be considered relatively common enteropathogens due to the increase of diseases in humans. aeromonas culicicola is a gram negative rod-shaped bacterium isolated for the first time from the mosquito mid-gut, but subsequently detected in other insects and waters also. our previous study discovered that a. culicicola harbors three plasmids, which we designated as pac3249a, pac3249b and pac3249c. we investigated and report here the existence and genetic organization ... | 2006 | 17205119 |
| genetic relationships of aeromonas strains inferred from 16s rrna, gyrb and rpob gene sequences. | genetic relationships among bacterial strains belonging to the genus aeromonas were inferred from 16s rrna, gyrb and rpob gene sequences. twenty-eight type or collection strains of the recognized species or subspecies and 33 aeromonas strains isolated from human and animal specimens as well as from environmental samples were included in the study. as reported previously, the 16s rrna gene sequence is highly conserved within the genus aeromonas, having only limited resolution for this very tight ... | 2006 | 17158971 |
| [properties of a triphenylmethane dyes decolorization enzyme (tpmd) from aeromonas hydrophila strain dn322]. | a novel bacterial enzyme for decolorization of triphenylmethane dyes from aeromonas hydrophila strain dn322 was purified and named tpmd. the basic properties of this enzyme including molecular weight, isoelectric point km as well as the optimum temperature and ph were determined and the enzyme was identified as an nadh/nadph-dependent oxygenase in previous research. based on previous results, the effect of different inhibitor including vc, metyrapone, rotenone, antimycin a and nan3 as well as th ... | 2006 | 17172037 |
| induction of vascular leakage and blood pressure lowering through kinin release by a serine proteinase from aeromonas sobria. | aeromonas sobria causes septic shock, a condition associated with high mortality. to study the mechanism of septic shock by a. sobria infection, we examined the vascular leakage (vl) activity of a. sobria serine proteinase (asp), a serine proteinase secreted by this pathogen. proteolytically active asp induced vl mainly in a bradykinin (bk) b(2) receptor-, and partially in a histamine-h(1) receptor-dependent manner in guinea pig skin. the asp vl activity peaked at 10 min to 1.8-fold of the initi ... | 2006 | 17142774 |
| x-ray absorption spectroscopy of the zinc-binding sites in the class b2 metallo-beta-lactamase imis from aeromonas veronii bv. sobria. | x-ray absorption spectroscopy was used to investigate the metal-binding sites of imis from aeromonas veronii bv. sobria in catalytically active (1-zn), product-inhibited (1-zn plus imipenem), and inactive (2-zn) forms. the first equivalent of zinc(ii) was found to bind to the consensus zn(2) site. the reaction of 1-zn imis with imipenem leads to a product-bound species, coordinated to zn via a carboxylate group. the inhibitory binding site of imis was examined by a comparison of wild-type imis w ... | 2006 | 17087519 |
| cloning of atp-dependent protease clpxp genes in aeromonas veronii. | 2006 | 17169116 | |
| influence of different concentrations of nitrogen and phosphorous on aeromonas spp. growth. | this paper evaluates the influence of nitrogen and phosphorous on the growth of aeromonas previously isolated from irrigation water. three strains respectively of three species of aeromonas (a. hydrophila, a. caviae, a. sobria) (nine strains) were individually inoculated in a microcosm added with ammonium, nitrate and phosphate using different combinations (nh4-n + no3-n and nh4-n + no3-n + po4-p) and concentrations (nh4-n: from 20 to 2500 microg l-1; no3-n: from 200 to 22000 microg l-1; po4-p: ... | 2006 | 17256017 |
| two unrelated cases of septicaemia due to vibrio cholerae non-o1, non-o139 in poland, july and august 2006. | 2006 | 17213560 | |
| secondary aeromonas peritonitis is associated with polymicrobial ascites culture and absence of liver cirrhosis compared to primary aeromonas peritonitis. | aeromonas peritonitis remains a rare condition. in this study we describe the clinical features of primary and secondary aeromonas peritonitis, and compare the differences between these two diseases entities. patients with aeromonas peritonitis were identified from microbiological and medical records during the period between march 1994 and march 2003. clinical characteristics, laboratory data, microbiological results, treatment and outcome of patients were obtained by retrospective chart review ... | 2006 | 17078857 |
| aeromonas agar is a useful selective medium for isolating aeromonads from faecal samples. | 2006 | 17030925 | |
| comparison of total and faecal coliforms as faecal indicator in eutrophicated surface water. | the aim of this study was to evaluate the use of total coliforms (tc) and faecal coliforms (fc) using a membrane filtration method for precise monitoring of faecal pollution in korean surface water. the samples were collected in korea from both main rivers and their tributaries. presumptive tc * fc were enumerated. the ratios of presumptive fc to tc were not constant, but varied widely, and tc were difficult to enumerate because of overgrowth by background colonies. for fc this was not the case. ... | 2006 | 17037151 |
| epidemiologic cutoff values for antimicrobial agents against aeromonas salmonicida isolates determined by frequency distributions of minimal inhibitory concentration and diameter of zone of inhibition data. | to develop epidemiologic cutoff values by use of frequency distributions for susceptibility to 4 antimicrobial agents when tested against a representative population of a major aquaculture pathogen, aeromonas salmonicida. | 2006 | 17078743 |
| viral and bacterial diseases of atlantic cod gadus morhua, their prophylaxis and treatment: a review. | this review summarises the state of knowledge of both viral and bacterial diseases of atlantic cod gadus morhua, and their diagnosis, prophylaxis and treatment. the most important losses have been at the larval and juvenile stages, and vibriosis has long been the most important bacterial disease in cod, with listonella (vibrio) anguillarum dominant among pathogenic isolates. vaccination of cod against pathogens such as l. anguillarum and aeromonas salmonicida clearly demonstrates that the cod im ... | 2006 | 17058605 |
| mutations within the catalytic motif of dna adenine methyltransferase (dam) of aeromonas hydrophila cause the virulence of the dam-overproducing strain to revert to that of the wild-type phenotype. | in this study, we demonstrated that the methyltransferase activity associated with dam was essential for attenuation of aeromonas hydrophila virulence. we mutated aspartic acid and tyrosine residues to alanine within the conserved dppy catalytic motif of dam and transformed the pbad/damd/a, pbad/damy/a, and pbad/damahssu (with the native dam gene) recombinant plasmids into the escherichia coli gm33 (dam-deficient) strain. genomic dna (gdna) isolated from either of the e. coli gm33 strains harbor ... | 2006 | 16988254 |
| updated phylogeny of the genus aeromonas. | recent phylogenetic studies of the genus aeromonas based on gyrb and rpod gene sequences have improved the phylogeny based on 16s rrna gene sequences first published in 1992, particularly in the ability to split closely related species. these studies did not include the recently described species aeromonas simiae and aeromonas molluscorum and only a single strain of aeromonas culicicola was available for analysis at that time. in the present work, these aeromonas species and newly isolated strai ... | 2006 | 17012583 |
| occurrence and diversity of integrons and beta-lactamase genes among ampicillin-resistant isolates from estuarine waters. | the aim of the present study was to assess the occurrence and molecular diversity of beta-lactamase genes and integrons among gram-negative ampicillin-resistant bacteria from ria de aveiro. ampicillin-resistant isolates were selected and subjected to genotyping using rep-pcr. representatives from each rep pattern were affiliated with the following taxa by sequencing the 16s rrna gene: aeromonas caviae, a. hydrophila, a. media, a. molluscorum, a. veronii, a. salmonicida, aeromonas sp., pseudomona ... | 2006 | 17125975 |
| bacterial aetiology of diarrhoea in young children: high prevalence of enteropathogenic escherichia coli (epec) not belonging to the classical epec serogroups. | diarrhoeal disease continues to be one of the most common causes of admittance in children hospital emergency. the aim of the present study was to investigate the relative contribution of enteropathogenic and enterohemorrhagic escherichia coli (epec and ehec, respectively) as a cause of infectious bacterial diarrhoea in children from the region of toulouse. we analysed 280 samples of stools from 280 children (<2 years) with diarrhoea admitted in the "hopital des enfants" from january to august 2 ... | 2006 | 17030460 |
| [prevalence of the bacteria causing diarrea in a rosario hospital, argentina]. | bacterial agents causing diarrea in patients attended in a hospital of rosario, argentina. the frequency of bacterial agents causing diarrhea can vary in patients of different areas, and through the time. in an epidemiological surveillance we studied 304 patients with diarrheal diseases looking for salmonella enterica, shigella spp, campylobacter spp, aeromonas spp, yersinia enterocolitica, and diarrheogenic escherichia coli. c. jejuni was isolated in 30 patients (9,9 %), salmonella in 18 (5,9 % ... | 2006 | 17639815 |
| aeromonas bacteremia in an elderly immunocompetent patient. | we report the case of an elderly immunocompetent patient with aeromonas hydrophila bacteremia without evidence of portal of entry. despite several risk factors for a poor outcome, such as impaired renal function, two positive blood cultures, and community-acquired infections, the patient survived. antimicrobial susceptibility was normal. unknown polycystic liver disease was discovered and misdiagnosed as a hepatic abscess at the time of the bacteremia which was confirmed by repeated ct scans. be ... | 2006 | 17063071 |
| [rapidly progressive myonecrosis by aeromonas veronii biotype sobria]. | myonecrosis due to aeromonas spp is exceptional. we report the case of a diabetic patient with liver cirrhosis who developed a rapidly progressive myonecrosis by aeromonas veronii biotype sobria. the portal of entry was an injury after falling down in an irrigation canal. the outcome was not favourable and surgical amputation of left leg was performed in spite of antibiotic treatment with cefotaxime and tobramicin. aeromonas spp can be very aggressive and this microorganism should be considered ... | 2006 | 17222071 |
| [classification and identification of vibrio cholerae and vibrio parahaemolyticus isolates based on gyrb gene phylogenetic analysis]. | in order to validate the usefulness of gyrb genotype for the classification and identification of vibrio cholerae and vibrio parahaemolyticus isolates, the phylogenetic analysis of 13 v. cholerae, 8 v. parahaemolyticus, 2 aeromonas hydrophila and 1 plesiomonas shigelloides strains was carried out using the partial coding sequence of gyrb, a gene that encodes the b subunit of dna gyrase (topoisomerase type ii ) in bacteria. these strains were separately clustered at species level and typed by the ... | 2006 | 17302148 |
| in vitro activity of telithromycin against gram-negative bacterial pathogens. | to investigate the in vitro activity of the ketolide anti-bacterial telithromycin against a range of commensal bacteria and common aerobic gram-negative respiratory and non-respiratory pathogens. | 2006 | 15996744 |
| effects of cyclophosphamide on the immune system and disease resistance of asian catfish clarias batrachus. | cyclophosphamide (cyp), a multifunctional alkylating agent is known as a potent immunosuppressor in endotherms. here, an experiment was conducted in an ectothermic asian catfish species, clarias batrachus to investigate its effect on non-specific and specific immunity as well as disease resistance against a common bacterial pathogen challenge. cyp was intraperitoneally injected for 3 consecutive days at a rate of 200 mgkg(-1) body weight. after 72 h post-injection, control and cyp-treated fish w ... | 2005 | 15863012 |
| a c1q-binding 40 kda porin from aeromonas salmonicida: cloning, sequencing, role in serum susceptibility and fish immunoprotection. | a 40 kda c1q-binding outer membrane protein from aeromonas salmonicida was identified by direct-binding assay with biotinylated c1q, a subcomponent of the complement classical pathway component c1. the 40 kda porin structural gene from the a450 a. salmonicida typical strain (a+:o+) was cloned in escherichia coli and sequenced. the amino acid sequence of the 40 kda a. salmonicida porin, its ability to bind c1q in an antibody independent process, and its immunological cross-reaction with the a. hy ... | 2005 | 15885977 |
| evaluation of an experimental aeromonas salmonicida epidemic in chinook salmon, oncorhynchus tshawytscha (walbaum). | to determine the dynamics of the transmission of aeromonas salmonicida ssp. salmonicida infection, chinook salmon, oncorhynchus tshawytscha, were exposed to bacteria by cohabitation. the latent period (time between exposure and infectivity) was determined by exposing a group of chinook salmonid fingerlings to a. salmonicida by bath, then, at daily intervals, by holding five exposed (donor) fish with approximately 50 naive fish for 24 h. the latent period was 3 days post-infection and the time pe ... | 2005 | 15892751 |
| development of a simple and rapid fluorogenic procedure for identification of vibrionaceae family members. | we describe a simple colony overlay procedure for peptidases (copp) for the rapid fluorogenic detection and quantification of vibrionaceae from seawater, shellfish, sewage, and clinical samples. the assay detects phosphoglucose isomerase with a lysyl aminopeptidase activity that is produced by vibrionaceae family members. overnight cultures are overlaid for 10 min with membranes containing a synthetic substrate, and the membranes are examined for fluorescent foci under uv illumination. fluoresce ... | 2005 | 16000757 |
| cloning and analysis of non-specific cytotoxic cell receptor (nccrp)-1 from common carp cyprinus carpio l. | nonspecific cytotoxic cell receptor protein (nccrp-1) provides an important function in target cell recognition and activation of cytotoxicity. nccrp-1 has been cloned from common carp cyprinus carpio l. from fish barbel by est analysis. the isolated gene is composed of 945 bp with a 79 bp 5' utr, 714 bp open reading frame and 152 bp 3' utr. the predicted nccrp-1 gene is composed of 237 amino acid residues and its predicted signal peptide is 19 amino acid residues in length. this gene has conser ... | 2005 | 15897012 |
| sepsis due to extended-spectrum beta-lactamase-producing aeromonas hydrophila in a pediatric patient with diarrhea and pneumonia. | 2005 | 16007554 | |
| [aeromonas caviae infection mimicking inflammatory bowel disease in a child]. | 2005 | 16008044 | |
| [aeromonas caviae infection mimicking inflammatory bowel disease in a child]. | 2005 | 16008045 | |
| aeromonas hydrophila-associated skin lesions and septicaemia in a nile crocodile (crocodylus niloticus). | aeromonas hydrophila is one of the most common bacteria associated with the aquatic environment. there are, however, limited data on a. hydrophila infection in crocodilians. the aim of this report is to describe a case of skin lesions and septicaemia associated with a. hydrophila in a nile crocodile (crocodylus niloticus). a captive male crocodile in the zoological park of antalya (turkey) was found dead without showing signs of any disease. gross examination showed brown or red-spotted skin les ... | 2005 | 15900900 |
| the impact of mutations in the quorum sensing systems of aeromonas hydrophila, vibrio anguillarum and vibrio harveyi on their virulence towards gnotobiotically cultured artemia franciscana. | disruption of quorum sensing, bacterial cell-to-cell communication by means of small signal molecules, has been suggested as a new anti-infective strategy for aquaculture. however, data about the impact of quorum sensing on the virulence of aquatic pathogens are scarce. in this study, a model system using gnotobiotically cultured artemia franciscana was developed in order to determine the impact of mutations in the quorum sensing systems of aeromonas hydrophila, vibrio anguillarum and v. harveyi ... | 2005 | 16011761 |
| polymorphism of aeromonas spp. trna intergenic spacers. | we investigated the length polymorphism of the intergenic spacers lying between trna genes of aeromonas spp. a total of 69 strains representing all known genomic species of aeromonas were used in the study. tdna-pcr patterns were examined by dice coefficient (s(d)) and unweighted pair group method of clustering (upgma). the strains were allocated into 15 groups at a similarity level of 70%. the strains belonging to seven genomic species: a. hydrophila (hg 1), a. caviae (hg 4), a. sobria (hg 7), ... | 2005 | 15900969 |
| molecular cloning, characterization and expression analysis of qm gene from grass carp (ctenopharyngodon idellus) homologous to wilms' tumor suppressor. | qm, a novel gene that was originally identified as a tumor suppressor, has been cloned from species encompassing members of higher vertebrate, plant and fungal kingdoms, but it is not well documented in fish. in present study, a gene homologous to qm was obtained from grass carp (ctenopharyngodon idellus) head kidney and spleen cdna library. the full-length grass carp qm (gcqm) cdna of 759 bp contains a short 5' utr of 22 bp, a 3' utr of 89 bp and an open reading frame of 648 nucleotides that tr ... | 2005 | 15936234 |
| antibacterial effect of cu2+-exchanged montmorillonite on aeromonas hydrophila and discussion on its mechanism. | montmorillonite (mmt) and its cu2+-exchanged montmorillonite (cu-mmt) were used to study the antibacterial activity on aeromonas hydrophila. the results indicated that mmt had no antibacterial activity. the minimum inhibitory concentration (mic) and bactericidal concentration (mbc) of cu-mmt on a. hydrophila are found to be 150 and 600 mg/l, respectively. the continuance of the antibacterial activity of cu-mmt was much longer than copper sulfate. in order to reveal the mechanism of the antibacte ... | 2005 | 15939555 |
| factors affecting biosurfactant production by oil degrading aeromonas spp. isolated from a tropical environment. | an aeromonas spp. was isolated from tropical estuarine water. the organism grew on crude oil and produced biosurfactant that could emulsify hydrocarbons. the peak growth and biosurfactant production was on the 8th day. the organism grew on a range of hydrocarbons that include crude oil and hexadecane while no growth was recorded on some hydrocarbons that include benzene. the biosurfactant produced by the organism emulsified a range of hydrocarbons with diesel (e24=65) as the best substrate and h ... | 2005 | 15878609 |
| quorum sensing signal molecules (acylated homoserine lactones) in gram-negative fish pathogenic bacteria. | the aim of the present study was to investigate the production of quorum sensing signals (specifically acylated homoserine lactones, ahls) among a selection of strains of gram-negative fish bacterial pathogens. these signals are involved in the regulation of virulence factors in some human and plant-pathogenic bacteria. a total of 59 strains, representing 9 different fish pathogenic species, were tested against 2 ahl monitor bacteria (agrobacterium tumefaciens nt1 [pzlr4] and chromobacterium vio ... | 2005 | 16042042 |
| clinical evaluation of an aerolysin-based screening test for paroxysmal nocturnal haemoglobinuria. | recently, a toxin produced by aeromonas hydrophila was demonstrated to bind directly to the glycosyl-phosphatidyl-inositol (gpi) anchor. after coupling it to a fluorescent dye and applying it in fluorescence-activated cell scanning (facs), this property was exploited to detect gpi-negative cells in the diagnosis of paroxysmal nocturnal haemoglobinuria (pnh). | 2005 | 15952213 |
| sex and low-level sampling stress modify the impacts of sewage effluent on the rainbow trout (oncorhynchus mykiss) immune system. | the objective of the present study was to investigate the influence of chronic exposure to municipal sewage treatment effluent at environmentally relevant concentrations on immune parameters in rainbow trout (oncorhynchus mykiss), including the assessment of potential differences in reactivity between sexually mature male and female fish. trout were exposed to 1.5 and 15% (v/v) secondary treated municipal sewage effluent for 32 weeks. fish were injected intra-peritoneally either with inactivated ... | 2005 | 15892994 |
| elucidation of the molecular structure of lipid a isolated from both a rough mutant and a wild strain of aeromonas salmonicida lipopolysaccharides using electrospray ionization quadrupole time-of-flight tandem mass spectrometry. | the chemical structure of lipid a, isolated by mild acid hydrolysis from a rough mutant and a wild strain of aeromonas salmonicida lipopolysaccharide, was investigated using electrospray ionization quadrupole time-of-flight (qqtof) hybrid tandem mass spectrometry and showed a great degree of microheterogeneity. the chemical structure of the main constituent of this heterogeneous mixture was identified as a beta-d-(1 --> 6) linked d-glucosamine disaccharide substituted by two phosphate groups, on ... | 2005 | 15912470 |
| adaptational changes in cellular fatty acid branching and unsaturation of aeromonas species as a response to growth temperature and salinity. | the effects of growth temperature and salinity on the cellular fatty acids were investigated on aeromonas caviae, aeromonas hydrophila and aeromonas sobria. under optimal growth conditions, fatty acids patterns were dominated by even-numbered chains c(16:0), c(16:1cis9), c(18:1cis11), c(12:0) and c(14:0). growth temperature modifications induced, in the three aeromonas species, important changes in fatty acid (i) unsaturation, (ii) branching and (iii) chain length. an important decrease in the c ... | 2005 | 15925007 |
| attenuated virulence of an aeromonas salmonicida subsp. salmonicida type iii secretion mutant in a rainbow trout model. | aeromonas salmonicida subsp. salmonicida is the causative agent of furunculosis, a severe systemic disease affecting salmonid fish. this bacterium contains a type iii protein secretion system that is responsible for the secretion and translocation of the adp-ribosylating toxin, aext, into the cytosol of fish cells. this study showed that inactivation of the type iii secretion system by marker-replacement mutagenesis of the gene ascv, which encodes an inner-membrane component of the type iii secr ... | 2005 | 15942017 |
| identification and characterization of putative virulence genes and gene clusters in aeromonas hydrophila ppd134/91. | aeromonas hydrophila is a gram-negative opportunistic pathogen of animals and humans. the pathogenesis of a. hydrophila is multifactorial. genomic subtraction and markers of genomic islands (gis) were used to identify putative virulence genes in a. hydrophila ppd134/91. two rounds of genomic subtraction led to the identification of 22 unique dna fragments encoding 19 putative virulence factors and seven new open reading frames, which are commonly present in the eight virulence strains examined. ... | 2005 | 16085838 |