Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| edwardsiella tarda-induced cytotoxicity depends on its type iii secretion system and flagellin. | many gram-negative bacteria utilize a type iii secretion system (t3ss) to translocate virulence proteins into host cells to cause diseases. in responding to infection, macrophages detect some of the translocated proteins to activate caspase-1-mediated cell death, called pyroptosis, and secretion of proinflammatory cytokines to control the infection. edwardsiella tarda is a gram-negative enteric pathogen that causes hemorrhagic septicemia in fish and both gastrointestinal and extraintestinal infe ... | 2014 | 24891103 |
| a multiplex pcr for the simultaneous detection of tenacibaculum maritimum and edwardsiella tarda in aquaculture. | a specific and sensitive multiplex pcr (mpcr) method was developed as a useful tool for the simultaneous detection of two important flatfish pathogens in marine aquaculture, tenacibaculum maritimum and edwardsiella tarda. in fish tissues, the average detection limit for these mpcr-amplified organisms was 2 × 10 ⁵ ± 0.2 cfu/g and 4 × 10 ⁵ ± 0.3 cfu/g, respectively. these values are similar or even lower than those previously obtained using the corresponding single pcr. moreover, mpcr did not prod ... | 2014 | 26418855 |
| lack of a contact requirement for direct antibacterial activity of lymphocyte subpopulations in ginbuna crucian carp. | cytotoxic t lymphocytes (ctl) recognize and kill cells infected with viruses, intracellular bacteria and tumors with mhc restriction and antigen specificity. in addition to these activities, recent studies in mammals have suggested that ctl can exhibit direct microbicidal activity. in our previous study we documented direct antibacterial activity of cd4(+) t cells and sigm(+) cells as well as cd8α(+) t cells from immunized fish. however, we also found weak non-specific killing activity of lympho ... | 2014 | 24859594 |
| expression and protective role of two novel nacht-containing proteins in pathogen infection. | lower vertebrates have been found to possess over 200 nacht-domain encoding genes; but, to date, very little is known about their functional activity. this article describes the sequences and expression analysis of two zebrafish nacht-containing proteins, namely nalpl1 and nalpl2. in addition, the functions of zebrafish nalpl1 and nalpl2, which are absent for both amino-terminal effector-binding domain (ebd) and carboxy-terminal ligand-recognition domain (lrd), were investigated for the first ti ... | 2014 | 24858030 |
| gc/ms-based metabolomics approach to identify biomarkers differentiating survivals from death in crucian carps infected by edwardsiella tarda. | microbial disease problems constitute the largest single cause of economic losses in aquaculture. an understanding of immune system in aquaculture animals how to function in defense against bacterial infections is especially important to control these diseases and improve food quality and safety. in the present study, we use a crucian carp model to explore which pathways and metabolites are crucial for the defense against infection caused by edwardsiella tarda eib202. we establish the metabolic ... | 2014 | 24837326 |
| molecular characterization and expression analysis of atp-gated p2x7 receptor involved in japanese flounder (paralichthys olivaceus) innate immune response. | atp-gated p2x7 receptor (p2rx7) channel is a key component for purinergic signaling and plays important roles in the innate immune response in mammals. however, the expression, molecular properties and immune significances of p2rx7 in lower vertebrates are still very limited. here we identified and characterized a novel bony fish p2rx7 homologue cdna, termed pop2rx7, in japanese flounder (paralichthys olivaceus). pop2rx7 protein shares about 60-88% sequence similarity and 45-78% sequence identit ... | 2014 | 24796752 |
| hu-induced polymorphous filamentation in fish pathogen edwardsiella tarda leading to reduced invasion and virulence in zebrafish. | edwardsiella tarda is a rod-shaped gram-negative pathogenic bacterium that causes hemorrhagic septicemia in fish. nucleoid-associated protein hu is a basic dna-binding protein with structural specificity in regulating genes expression. in wild-type e. tarda eib202, hu is composed of two subunits huα (hupa) and huβ (hupb), and exists in homodimer or heterodimer forms. different from the wild-type and δhupb mutant, δhupa mutant was found to be defective in cell growth, h2s production, acid adaptat ... | 2014 | 24793099 |
| multilocus variable number tandem repeat analysis of edwardsiella piscicida isolates pathogenic to fish. | this study describes a novel multilocus variable number tandem repeat analysis (mlva) based on six variable number of tandem repeat (vntr) loci for genotyping of 37 edwardsiella piscicida (previously edwardsiella tarda) isolates from multiple sources. the number of alleles identified for each of the six vntr loci ranged from 3 to 5 with vntr loci 1 (di = 0.632) and 3 (di = 0.644), displaying the highest degrees of polymorphism. mlva typing of the 37 e. piscicida isolates resulted in the identifi ... | 2014 | 24749655 |
| cell-penetrating peptides mediated protein cross-membrane delivery and its use in bacterial vector vaccine. | it is an attractive strategy to develop a recombinant bacterial vector vaccine by expressing exogenous protective antigen to induce the immune response, and the main concern is how to enhance the cellular internalization of antigen produced by bacterial vector. cell-penetrating peptides (cpps) are short cationic/amphipathic peptides which facilitate cellular uptake of various molecular cargoes and therefore have great potentials in vector vaccine design. in this work, eleven different cpps were ... | 2014 | 24746937 |
| identification and expression analysis of lipopolysaccharide-induced tnf-alpha factor gene in chinese mitten crab eriocheir sinensis. | lipopolysaccharide-induced tnf-alpha factor (litaf) is an important transcription factor in transcriptional regulation of tnf-alpha and other cytokines. here we identified a full-length litaf homolog cdna, termed eslitaf, that contains a 71 bp 5'-untranslated sequence, an open reading frame consisting of 381 bp, and a 208 bp 3'-untranslated sequence in chinese mitten crab (eriocheir sinensis), an economically important freshwater crustacean species in china. eslitaf protein possesses a conserved ... | 2014 | 24657317 |
| impact of co-deficiency of rpon and rpos on stress tolerance, virulence and gene regulation in edwardsiella tarda. | edwardsiella tarda the etiological agent for edwardsiellosis, a devastating fish disease prevailing in worldwide aquaculture industries was subjected to a molecular genetic study. to research into the influence when rpon (σ(54) ) and rpos (σ(38) ) were deleted simultaneously, the double deletion mutant of rpon (σ(54) ) and rpos (σ(38) ), namely rnrs, was constructed. firstly, rpon and rpos are both essential for h2 o2 , starvation, high osmotic pressure and acid resistance, which have synergisti ... | 2014 | 24633758 |
| a mutation in rcsb, a gene encoding the core component of the rcs cascade, enhances the virulence of edwardsiella tarda. | edwardsiella tarda, a gram-negative bacterium of the family enterobacteriaceae, is the causative agent of the systemic disease edwardsiellosis, which is a major problem in aquaculture industry worldwide. many virulence-related genes in e. tarda have been investigated, but the rcs phosphorelay, a two-component pathway, which regulates several cell-surface-associated structures related to invasion and survival in host cells, has not yet been thoroughly studied. in the present study, an rcsb in-fra ... | 2014 | 24631591 |
| eha, a transcriptional regulator of hemolytic activity of edwardsiella tarda. | hemolysis causes major symptoms such as the reddening skin and systemic hemorrhagic septicemia of diseased fish infected by edwardsiella tarda. cytolysin a (clya) is a pore-forming cytotoxic protein encoded by the clya gene in escherichia coli k-12. in this study, we observed that the heterologous expression of the eha gene from e. tarda could confer hemolytic activity upon a hemolytic-silent e. coli strain. the transcription of clya is positively controlled by the eha gene in e. tarda by rt-pcr ... | 2014 | 24628619 |
| cloning and expression analyses of interferon regulatory factor (irf) 3 and 7 genes in european eel, anguilla anguilla with the identification of genes involved in ifn production. | interferon regulatory factor (irf) 3 and irf7 have been identified as regulators of type i interferon (ifn) gene expression in mammals. in the present study, the two genes were cloned and characterized in the european eel, anguilla anguilla. the full-length cdna sequence of irf3 and irf7 in the european eel, named as aairf3 and aairf7 consists of 2879 and 2419 bp respectively. multiple alignments showed that the two irfs have a highly conserved dna binding domain (dbd) in the n terminus, with th ... | 2014 | 24565894 |
| effect of edwardsiella tarda immunization on systemic immune response, mucosal immune response and protection in catla (catla catla). | the effect of immunization on systemic and cutaneous mucosal immune responses of fish and their possible relation with protection has not been fully assessed. in this study, healthy catla (catla catla) were immunized against edwardsiella tarda using two antigenic preparations namely, whole cell bacterin (b) and bacterin mixed with freund's complete adjuvant in a 1:1 (v/v) ratio (b+a) followed by a booster dose after 3 weeks of first injection. different systemic and cutaneous mucosal immune resp ... | 2014 | 24477841 |
| molecular cloning and expression analysis of two lipopolysaccharide-induced tnf-α factors (litafs) from rock bream, oplegnathus fasciatus. | lipopolysaccharide (lps)-induced tumor necrosis factor (tnf)-α factor (litaf) plays an important role controlling the expression of tnf-α and the other cytokine genes in the presence of lps. however, two litaf homologues have not been characterized in fish. in this study, we cloned two distinct litaf (rblitaf1 and rblitaf2) cdnas from rock bream (oplegnathus fasciatus) and characterized their expression profiles after infection with edwardsiella tarda, streptococcus iniae or red seabream iridovi ... | 2014 | 24394623 |
| development of a multiplex pcr assay to detect edwardsiella tarda, streptococcus parauberis, and streptococcus iniae in olive flounder (paralichthys olivaceus). | a multiplex pcr protocol was established to simultaneously detect major bacterial pathogens in olive flounder (paralichthys olivaceus) including edwardsiella (e.) tarda, streptococcus (s.) parauberis, and s. iniae. the pcr assay was able to detect 0.01 ng of e. tarda, 0.1 ng of s. parauberis, and 1 ng of s. iniae genomic dna. furthermore, this technique was found to have high specificity when tested with related bacterial species. this method represents a cheaper, faster, and reliable alternativ ... | 2014 | 24378589 |
| identification and characterization of lipopolysaccharide-induced tnf-alpha factor gene from japanese flounder paralichthys olivaceus. | lipopolysaccharide-induced tnf-α factor (litaf) is an important transcription factor participating in innate immunity through regulating tnf-α and other inflammatory cytokines expression. however, the expression and biological relevance of litaf in fish is still very limited. in this study, a full-length litaf cdna, termed politaf, was identified from japanese flounder paralichthys olivaceus. politaf contains a 67 bp 5'-untranslated sequence, a 435 bp open reading frame, and a 647 bp 3'-untransl ... | 2014 | 24359872 |
| first isolation and characterization of edwardsiella tarda from diseased striped catfish, pangasianodon hypophthalmus (sauvage). | 2014 | 24344765 | |
| edwardsiella tarda superinfection in relapse of ulcerative colitis. | 2014 | 24326755 | |
| outer membrane protein assembly factor yaet (omp85) and groel proteins of edwardsiella tarda are immunogenic antigens for labeo rohita (hamilton). | 2014 | 24320972 | |
| role of cd4(+) and cd8α(+) t cells in protective immunity against edwardsiella tarda infection of ginbuna crucian carp, carassius auratus langsdorfii. | edwardsiella tarda is an intracellular pathogen that causes edwardsiellosis in fish. our previous study suggests that cell-mediated immunity (cmi) plays an essential role in protection against e. tarda infection. in the present study, we adoptively transferred t-cell subsets sensitized with e. tarda to isogenic naïve ginbuna crucian carp to determination the t-cell subsets involved in protecting fish from e. tarda infection. recipients of cd4(+) and cd8α(+) cells acquired significant resistance ... | 2014 | 24316500 |
| in situ total aortic arch replacement for infected distal aortic arch aneurysms with penetrating atherosclerotic ulcer. | we present a series of patients who underwent in situ total aortic arch replacement for infected distal aortic arch aneurysms. | 2014 | 24607377 |
| complete genome sequence of the edwardsiella ictaluri-specific bacteriophage pei21, isolated from river water in japan. | we present the complete genome sequence for a novel edwardsiella ictaluri-specific bacteriophage, pei21, isolated from river water in japan. an initial comparative genome analysis revealed that the phage was closely related to the previously reported edwardsiella tarda phage msw-3 isolated from a red sea bream farm in japan. | 2014 | 24699953 |
| phenotypic traits, virulence-associated gene profile and genetic relatedness of edwardsiella tarda isolates from japanese eel anguilla japonica in korea. | edwardsiella tarda is the predominant bacterium in farm-cultured eel in korea. here, we evaluated the heterogeneity of 37 e. tarda isolates derived from japanese eel with various origins (olive flounder, common carp and ornamental fish) between 2003 and 2010. regardless of origins, the biochemical characteristics of e. tarda isolates were homogenous except hydrogen sulfide production, citrate utilization and mannitol fermentation. based on the phylogenetic analysis of 16s rrna, e. tarda isolates ... | 2014 | 24117973 |
| endoscopic ultrasonography-guided transmural drainage of an infected hepatic cyst due to edwardsiella tarda: a case report. | infected hepatic cysts are very rare compared to simple liver cysts and abscesses. we treated a 77-year-old man with an infected hepatic cyst in the lateral segment caused by edwardsiella tarda, which has not been previously reported as a pathogenic organism associated with infected hepatic cysts. percutaneous drainage was temporarily effective, but infection recurred after the drainage tube was removed. we then inserted two drainage tubes into the cyst using an endoscopic ultrasonography (eus)- ... | 2014 | 26184023 |
| mutations of flagellar genes flic12, flia and flhdc of edwardsiella tarda attenuated bacterial motility, biofilm formation and virulence to fish. | the aim of this study was to investigate functions of flagellar genes flic2, flic12, flia and flhdc in a bacterial fish pathogen edwardsiella tarda. | 2014 | 24118854 |
| in vitro quenching of fish pathogen edwardsiella tarda ahl production using marine bacterium tenacibaculum sp. strain 20j cell extracts. | quorum quenching (qq) has become an interesting alternative for solving the problem of bacterial antibiotic resistance, especially in the aquaculture industry, since many species of fish-pathogenic bacteria control their virulence factors through quorum sensing (qs) systems mediated by n-acylhomoserine lactones (ahls). in a screening for bacterial strains with qq activity in different marine environments, tenacibaculum sp. strain 20j was identified and selected for its high degradation activity ... | 2014 | 24695235 |
| enterobacteria identification and detection of diarrheagenic escherichia coli in a port complex. | the port complex of maranhão (pcm) is the second largest port complex in brazil, receiving ships with large volumes of ballast water. to evaluate the microbiological quality of its waters, physicochemical parameters (ph and salinity), the number of coliforms (thermotolerants and totals), and the presence of enterobacterias and diarrheagenic escherichia coli strains were analyzed. in order to identify the presence of e. coli virulence genes target regions of the stx, elt, est, aggr, cvd432, ipah ... | 2014 | 25477930 |
| edwardsiella tarda mlic, a lysozyme inhibitor that participates in pathogenesis in a manner that parallels ivy. | edwardsiella tarda, a bacterial pathogen to farmed fish as well as humans, possesses the genes of two lysozyme inhibitors, ivy and mlic (ivy(et) and mlic(et)). we recently studied ivyet and found it to be implicated in e. tarda virulence. in the present study, we characterized mlic(et) in comparison with ivy(et) in a turbot model. mlic(et) contains the fwskg motif and two cysteines (c33 and c98) that are highly conserved in subgroup 1 mlics but are of unknown functional importance. to examine th ... | 2014 | 25404031 |
| edwardsiella tarda hfq: impact on host infection and global protein expression. | hfq is an rna-binding protein that plays an important role in many cellular processes. in this study, we examined the biological effect of the hfq of edwardsiella tarda, a severe fish pathogen with a broad host range that includes humans. to facilitate the study, a markerless hfq in-frame deletion wild type, txhfq, was constructed. compared to the wild type tx01, txhfq exhibited (i) retarded planktonic and biofilm growth, (ii) decreased resistance against oxidative stress, (iii) attenuated overa ... | 2014 | 24568370 |
| [thyrotoxic crisis]. | 2014 | 4193806 | |
| an unusual cause of neck swelling in an elderly female. | 2014 | 4083241 | |
| [the 58th annual meeting of the japanese orthopedic association. gifu, april 7-9, 1985. abstracts]. | 2014 | 4078426 | |
| introduction to panel: perspectives on the nature of psychic reality. | 2014 | 4056297 | |
| effects of hydralazine on pressure-volume and stress-volume relations in congestive heart failure secondary to idiopathic dilated cardiomyopathy. | the mechanism by which hydralazine improves cardiac function in patients with heart failure is not well characterized. hydralazine may improve left ventricular (lv) function by decreasing afterloading wall stress or by increasing myocardial contractility. the effect of intravenous hydralazine was assessed in 8 patients with severe idiopathic dilated cardiomyopathy. hydralazine increased stroke volume index (from 24 +/- 8 to 40 +/- 9 ml/m2, p less than 0.01) and decreased systemic vascular resist ... | 2014 | 4050708 |
| prison-psychiatry: medical or correctional discipline? | 2014 | 4010493 | |
| [a case of primary adenocarcinoma of the cervix of probable origin from an area of endometriosis]. | a case of primary adenocarcinoma of the cervix of probable endometrial origin is described in a 37 year-old woman. | 2014 | 3974935 |
| low doses of progestogen as an approach to fertility control. | 2014 | 4158852 | |
| what is visual training: a patient education handout. | 2014 | 3968385 | |
| receptive-field symmetry probed using converging gratings. | contrast-sensitivity functions (csf's) for converging and diverging gratings were obtained under voluntary fixation at several field widths and under retinal stabilization at one field width. in general, these types of gratings had similar csf's at all temporal frequencies tested, a result that can be explained by plausible spatiotemporal receptive-field models possessing spatial antisymmetry but not by models possessing pure (even) spatial symmetry. phase condition at the central line in conver ... | 2014 | 3973760 |
| [mechanisms of adaptation to physical exertion in patients with an expiratory obstructive syndrome]. | 2014 | 4096445 | |
| toxicokinetics of lithium intoxication treated by hemodialysis. | a 66-year old female on long-term lithium therapy, developed neurologic toxicity with coma and convulsions. the treatment consisted artificial ventilation, fluid and electrolyte infusions and a six hour hemodialysis (hd) on days 2, 3 and 4. the convulsions disappeared on day 4; the patient regained consciousness on day l2 and recovered with slight sequelae. toxicokinetic studies were conducted for a period of 12 days. the lithium (li) serum concentration fell from 4.4 mmol/l on admission to 2.16 ... | 2014 | 3938485 |
| measurement of color thresholds. | it is generally believed that some degree of defective color vision is frequently acquired along with certain ocular and systemic disorders. precise definition of the nature and extent of the color defects has not been possible because of the limitations inherent in the tests currently available for clinical use. we undertook to define the defects in terms of thresholds of discrimination to each color, plotted on a color circle similar to munsell's uniform chromaticity scale diagram. furthermore ... | 2014 | 3942184 |
| agglutination of an edta blood sample caused by an edta-dependent panagglutinin. | a novel example of an edta-dependent panagglutinin is described. a blood sample drawn into edta for a routine hematologic workup demonstrated strong agglutination due to edta-dependent panagglutinins. previous examples have been detected because of discrepant abo results. this is the first report of an edta-dependent panagglutinin that caused agglutination in the edta sample collection tube and a false positive direct antiglobulin test. | 2014 | 3920896 |
| [sequential angio-urography (sau) with photographic image subtraction]. | urography was performed in 250 patients: the technique of bolus injection of a large quantity of contrast medium was used in every case. by means of rapid seriography the early vascular phase was shown and reproduced, when necessary, with image subtraction. the abdominal aorta and the renal arteries were demonstrated in 97.6% of cases. altogether, in 34.4% of cases the existence of pathological conditions non demonstrable by conventional urography was ascertained; in 12.4% of cases extrarenal pa ... | 2014 | 3911305 |
| [medical education in the 18th century. on the 325th anniversary of the birth of friedrich hoffmann (1660-1742)]. | the 325th anniversary of friedrich hoffmann's birthday gave rise to remind of the early high period of the faculty of medicine of halle university. several significant scientific achievements are connected with hoffmann's name, but at the same time also the organisation of studies and examinations in halle. the educational practices of his time were demonstrated with the help of preserved documentations. | 2014 | 3904240 |
| [rare tumors of the iris and ciliary body. i]. | 2014 | 4187762 | |
| [metabolism of malignant melanomas. biochemical studies]. | 2014 | 4346243 | |
| cretinism with muscular hypertrophy (kocher-debré-sémélaigne syndrome). histochemical and ultrastructural study of skeletal muscle. | 2014 | 4097646 | |
| triple arthrodesis. a critical long-term review. | the results of triple arthrodesis performed in 80 feet and followed for an average of 13 years are reviewed. although the majority of patients were very pleased, the results of objective assessment were less favourable. there was a high incidence of degenerative joint changes in the ankle and midfoot and also of pseudarthrosis, avascular necrosis of the talus and residual deformity. pre-operative rigid equinovarus deformity produced the majority of the poor results. it is suggested that bony res ... | 2014 | 3958012 |
| relationship of eeg background rhythms to photic evoked responses. | 2014 | 4157834 | |
| [carpal tunnel syndrome in dialysis patients]. | 2014 | 4022204 | |
| [paget's disease of the nipple and mammary epithelioma. importance of mammography in diagnosis]. | 2014 | 4294240 | |
| [paget's disease of the nipple and mammary epithelioma. importance of mammography in diagnosis]. | 2014 | 4294240 | |
| selective surgical management of neural tube malformations. | the physical characteristics of 49 children with spina bifida cystica, survivors of a group subjected to selection for early surgery are compared with 39 children alive from an earlier unselected series, born in the 1960s, and reviewed retrospectively. sixteen children were also studied in whom the initial decision not to operate had been followed by survival and subsequent treatment. children selected for initial surgery have a significantly lower mortality than those not selected and their mob ... | 2014 | 4015145 |
| iron losses in sweat. | the losses of iron in whole body cell-free sweat were determined in eleven healthy men. a new experimental design was used with a very careful cleaning procedure of the skin and repeated consecutive sampling periods of sweat in a sauna. the purpose was to achieve a steady state of sweat iron losses with minimal influence from iron originating from desquamated cells and iron contaminating the skin. a steady state was reached in the third sauna period (second sweat sampling period). iron loss was ... | 2014 | 3953482 |
| amiphenazole sensitivity with oral ulceration. | 2014 | 4224132 | |
| [the acth-oxytocin association in the treatment of edematigenous cerebral diseases]. | 2014 | 4319047 | |
| [the acth-oxytocin association in the treatment of edematigenous cerebral diseases]. | 2014 | 4319047 | |
| electron microscopic and histochemical observations on the pulmonary alveolar surfactant in normal and irradiated mice. | 2014 | 4244539 | |
| [curare-like effect of african strychnos: strychnos usambarensis gilg du rwanda]. | 2014 | 4245543 | |
| [radiation injury and lege artis performed roentgenologic examinations of infants and children, together with a proposal for professional training]. | 2014 | 4234509 | |
| propoxyphene. two fatalities due to overdose. | 2014 | 4684062 | |
| [circadian rhythms of induced ethanolemia parameters in six healthy young adult males]. | 2014 | 4210897 | |
| preparative electrophoresis of serum proteins on plaster of paris. | 2014 | 4177769 | |
| physiological stress and performance changes in response to beginning level orienteering. | 2014 | 4068683 | |
| [use of adhesive cellophane tape in the diagnosis of demodicosis]. | 2014 | 4072051 | |
| [levels of immunoglobulins g,a,m,d and e in the current population of children]. | 2014 | 4217659 | |
| the language of dementia in the elderly: a pilot study. | 2014 | 4052318 | |
| british medical association attacks cigarette smoking. | 2014 | 3978493 | |
| evaluation and treatment of chronic simple dementias. | 2014 | 4256078 | |
| [growth of small murine rodents under laboratory conditions (the example of voles of the genus clethrionomys)]. | 2014 | 3911636 | |
| tissue immunity and organ transplantation. | 2014 | 4172388 | |
| prof. frits grewel, 1898-1973. | 2014 | 4601331 | |
| typology of male-to-female transsexualism. | this study tested a prediction derived from the hypothesis that asexual and bisexual transsexualism are actually subtypes of heterosexual transsexualism. two questionnaire scales measuring erotic attraction to males and females were administered to 163 male-to-female transsexuals. a cluster analysis of their scores divided the subjects into four groups: heterosexual, homosexual, bisexual, and asexual. fisher exact tests were used to compare the frequency with which subjects in the four clusters ... | 2014 | 4004548 |
| activities of the modified polyene n-d-ornithyl amphotericin methyl ester and the azoles ici 153066, bay n 7133, and bay l 9139 compared with those of amphotericin b and ketoconazole in the therapy of experimental blastomycosis. | we studied the efficacy of new experimental antifungal drugs, which represent molecular modifications of present active agents, in a murine model of blastomycosis. ketoconazole, previously the best azole drug studied and which is protective when administered orally, was superior to a new oral imidazole, bay l 9139, and a new oral triazole, bay n 7133. a new oral triazole, ici 153066, was markedly more effective than ketoconazole and is the only oral drug studied which came close to producing com ... | 2014 | 3994350 |
| automatic external defibrillation: bringing it home. | 2014 | 4063027 | |
| hypertrophy of heart with outflow tract obstruction and myocardial necrosis in an infant with multiple congenital malformations. | 2014 | 4252909 | |
| identification of the bicarbonate secretory cell of the turtle bladder. | the turtle bladder consists primarily of two mucosal cell types, mitochondrial rich (mr) and granular (g) cells. the mr cells secrete h+. in addition, the bladder secretes hco3-, an energy-requiring cl(-)-dependent transport system. these studies were designed to identify the cell type responsible for hco3- secretion. specific hco3- and h+ secretory inhibitors were added to alter o2 consumption of mr and g cells. cells were separated by ficoll density centrifugation. ouabain (10(-4) m) was used ... | 2014 | 4073269 |
| two nf-κb inhibitor-alpha (iκbα) genes from rock bream (oplegnathus fasciatus): molecular characterization, genomic organization and mrna expression analysis after immune stimulation. | ikba is a member of ikb family, which sequesters nf-kb in an inactivate form in the cytoplasm and blocks the translocation of nf-kb to nucleus. the ikba paralogs of rock bream (ofikba-a and ofikba-b) encoded ikba proteins with typical features including, highly conserved ikb degradation motif, six ankyrin repeats and a pest sequence. however, their amino acid identity and similarity were only 55.6 and 69.7%, respectively suggesting that these two genes could be the two different isoforms of ikba ... | 2014 | 25462458 |
| two carboxypeptidase counterparts from rock bream (oplegnathus fasciatus): molecular characterization, genomic arrangement and immune responses upon pathogenic stresses. | carboxypeptidases (cps) are proteases that hydrolyze c-terminal peptide bonds. they are involved in regulating the complement system of the immune system. here, we report the molecular characterization and immune response of two carboxypeptidases, named carboxypeptidase a (rb-cpa) and carboxypeptidase n1 (rb-cpn1), from rock bream. the genomic sequence of rb-cpa contains 12 exons interrupted by 11 introns, while the genomic sequence of rb-cpn1 has 9 exons and 8 introns. the cdna sequence of rb-c ... | 2014 | 25457548 |
| adhesive and invasive capacities of edwardsiella tarda isolated from south american sea lion. | edwarsiella tarda is a zoonotic bacterium that can be isolated from humans, animals and the environment. although e. tarda is primarily considered a fish pathogen, it is the only species of its genus considered to be pathogenic for humans as well. a survey of zoonotic intestinal bacteria in fresh feces from south american sea lions (sasl) otaria flavescens, reported e. tarda as the most frequently isolated species. in this study, we used hep-2 cells to establish in vitro the adherence and invasi ... | 2014 | 25477948 |
| a manganese superoxide dismutase with potent antioxidant activity identified from oplegnathus fasciatus: genomic structure and transcriptional characterization. | in this study, we describe the identification and characterization of manganese superoxide dismutase, an important antioxidant enzyme acting as the chief reactive oxygen species (ros) scavenger, from rock bream oplegnathus fasciatus (of-mmnsod) at genomic- and transcriptional-levels as well as the biological activity of recombinant protein. the of-mmnsod protein portrayed distinct mnsod family features including signature motifs, metal association sites and the typical active site topology. it w ... | 2013 | 23022055 |
| identification of novel interleukin 1 beta family genes in japanese flounder paralichthys olivaceus. | gene in the interleukin 1 family plays a central role in the regulation of immune and inflammatory responses. here, we describe two novel il-1-like gene in japanese flounder paralichthys olivaceus (jfil1-l1 and jfil1-l2). jfil1β-l1 was homologous to nile tilapia il-1β-like gene and arctic char il-1, and jfil1β-l2 showed homology to hypothetical protein loc100699119 of nile tilapia and rainbow trout oncorhynchus mykiss il-1 receptor agonist (ra). the deduced amino acids sequences of these il-1β-l ... | 2013 | 23069785 |
| genomic characterization and expression analysis of complement component 8α and 8β in rock bream (oplegnathus fasciatus). | the complement component 8α and 8β are glycoproteins that mediate formation of the membrane attack complex (mac) on the surface of target cells. full-length complement c8α (rb-c8α) and c8β (rb-c8β) sequences were identified from a cdna library of rock bream (oplegnathus fasciatus), and their genomic sequences were obtained by screening and sequencing of a bacterial artificial chromosome (bac) genomic dna library of rock bream. the rb-c8α gene contains 64bp of 5'-utr, open reading frame (orf) of ... | 2013 | 23059376 |
| functional characterization of edwardsiella tarda twin-arginine translocation system and its potential use as biological containment in live attenuated vaccine of marine fish. | bacterial twin-arginine translocation (tat) system contributes to translocate folded proteins to the periplasm and plays pleiotropic roles in physiological fitness. here, we showed that the fish pathogen edwardsiella tarda tat pathway was functional and was essential for h2s production and hemolytic activity. e. tarda tat mutant was more susceptible to diverse stresses such as high temperature, sds, ethanol, and high-salt conditions. however, e. tarda tat mutant displayed marginal in vivo virule ... | 2013 | 23053108 |
| effects of increased cell mass and altered gene dosage on the timing of initiation of macronuclear dna synthesis in paramecium tetraurelia. implications for cell cycle regulation. | in paramecium, cell mass and macronuclear dna content can vary substantially, and both variables affect the timing of initiation of macronuclear dna synthesis. cells normally begin macronuclear dna synthesis at 0.25 in the cell cycle when the mean cell mass is about 119% of the initial value. gene mutations were used to alter cell size by temporarily blocking cell cycle progression and to change dna content by altering the segregation pattern of macronuclear material to daughter nuclei at fissio ... | 2013 | 3709689 |
| the role of the clinical environment in the translation of research into practice. | 2013 | 3636224 | |
| [congress 86. there is nothing too small to be discussed]. | 2013 | 3638012 | |
| successful pregnancy in primary glomerular disease. | the course of 66 pregnancies was studied in 48 women with primary glomerular diseases. in all cases diagnoses were established by biopsy before pregnancy. they were: membranoproliferative glomerulonephritis in 16 patients, focal glomeruloesclerosis in 13, iga nephropathy in 10, membranous nephropathy in seven and focal glomerulonephritis in two women. the clinical status of the nephropathy before conception was that 43 had only mild renal dysfunction, five had moderate renal insufficiency, serum ... | 2013 | 3820941 |
| [cerebral hemorrhage in the premature neonate. report of 80 cases]. | 2013 | 3792879 | |
| association-dissociation equilibria of octopus hemocyanin. | the equilibria between the native (decameric) octopus hemocyanin and its subunits were studied by analytical sedimentation. equilibrium is obtained slowly, but the reaction is thermodynamically reversible. the mass action law for a monomer-decamer reaction is obeyed. the reassociated hemocyanin is virtually identical in its sedimentation behavior and oxygen binding with the native protein. the association-dissociation equilibria are mediated by cations; mg2+, ca2+, na+, and h+ are all effective ... | 2013 | 4063339 |
| variations in the preservation of bank blood due to processing. | 2013 | 4642160 | |
| variations in the preservation of bank blood due to processing. | 2013 | 4642160 | |
| the value of stimulus detection for long periods in the diagnosis of spasmophilia. | 2013 | 4188498 | |
| the value of stimulus detection for long periods in the diagnosis of spasmophilia. | 2013 | 4188498 | |
| the cutting diathermy and drain tracts. | 2013 | 3810377 | |
| the john scott memorial lecture 1985. space invaders. | 2013 | 3749489 | |
| refinement of the solution structure of the dna hexamer 5'd(gcatgc)2: combined use of nuclear magnetic resonance and restrained molecular dynamics. | the solution structure of the self-complementary dna hexamer 5'd(gcatgc)2 comprising the specific target site for the restriction endonuclease sph 1 is investigated by using nuclear magnetic resonance spectroscopy and restrained molecular dynamics. all the nonexchangeable proton resonances are assigned sequentially, and from time-dependent nuclear overhauser enhancement measurements a set of 158 approximate interproton distances are determined. these distances are used as the basis of a structur ... | 2013 | 3651407 |