Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| expression and functional characterization of vitronectin gene from japanese flounder (paralichthys olivaceus). | vitronectin (vtn) is a multifunctional protein that plays significant roles in cell adhesion, migration, spreading and survival, and in the regulation of membrane attack complex formation and the terminal pathway of complement activation in innate immune response. however, the expression and immune significance of vtn in fish remains largely unknown. in order to understand the involvement of vtn in fish innate immune response, here we cloned and characterized a full-length vtn ortholog cdna, ter ... | 2017 | 28377270 |
| a homolog of teleostean signal transducer and activator of transcription 3 (stat3) from rock bream, oplegnathus fasciatus: structural insights, transcriptional modulation, and subcellular localization. | signal transducer and activator of transcription 3 (stat3) is one of the crucial transcription factors in the janus kinase (jak)/stat signaling pathway, and it was previously considered as acute phase response factor. a number of interleukins (ils) such as il-5, il-6, il-9, il-10, il-12, and il-22 are known to be involved in activation of stat3. in addition, various growth factors and pathogenic or oxidative stresses mediate the activation of a wide range of functions via stat3. in this study, a ... | 2017 | 28413047 |
| corrigendum to "glucose enhances tilapia against edwardsiella tarda infection through metabolome reprogramming" [fish and shellfish immunol. 61 (2017) 34-43]. | 2017 | 28416091 | |
| molecular cloning, characterization and expression analysis of heat shock protein 90 in albino northern snakehead channa argus. | the great albino northern snakehead channa argus is habitual to only the sichuan jialing rivers system in china, making its introduction difficult to other riverine systems. here, we characterized heat shock protein 90 (acahsp90) and probed its molecular responses toward the environmental stressors that c. argus can face during its introduction and breeding in the other southern latitudes of china. to serve the purpose, cdna encoding of acahsp90 were cloned and characterized in albino c. argus. ... | 2017 | 28442397 |
| characterization of toll-like receptor 22 in turbot (scophthalmus maximus). | toll-like receptors (tlrs) are essential for activation of the innate immune system in response to invading pathogens. in this paper, expression profiles of the turbot (scophthalmus maximus) tlr22 gene (tbtlr22) were analyzed with rt-pcr and in situ hybridization. then its expression patterns simulated with ligands or pathogens were investigated. streptococcus iniae, edwardsiella tarda, hirame rhabdovirus virus (hirrv), polyinosinic: polycytidylic acid (poly i:c), peptidoglycan (pgn), or lipopol ... | 2017 | 28495564 |
| molecular cloning, transcriptional profiling, and subcellular localization of signal transducer and activator of transcription 2 (stat2) ortholog from rock bream, oplegnathus fasciatus. | signal transducer and activator of transcription 2 (stat2) is a key element that transduces signals from the cell membrane to the nucleus via the type i interferon-signaling pathway. although the structural and functional aspects of stat proteins are well studied in mammals, information on teleostean stats is very limited. in this study, a stat paralog, which is highly homologous to the stat2 members, was identified from a commercially important fish species called rock bream and designated as r ... | 2017 | 28499943 |
| proteome profiling reveals immune responses in japanese flounder (paralichthys olivaceus) infected with edwardsiella tarda by itraq analysis. | the liver is an important organ for bacterial pathogen attack in fish. the differential proteomic response of the japanese flounder liver to edwardsiella tarda infection was examined using isobaric tags for relative and absolute quantitation (itraq) labeling followed by liquid chromatography-tandem mass spectrometry (lc-ms/ms). a total of 3290 proteins were identified and classified into categories related to biological process (51.4%), molecular function (63.6%), and cellular component (57.7%). ... | 2017 | 28511951 |
| draft genome sequence of the fish strain edwardsiella tarda ncimb 2034. | edwardsiella tarda is an important pathogen for fish. the strain ncimb 2034, obtained from the national collection of industrial food and marine bacteria, was isolated from unknown diseased fish in the united states. the draft genome sequence has 3.79 mb with a g+c content of 57.1% and >3,340 protein-coding genes. | 2017 | 28522714 |
| molecular characterization of mudskipper (boleophthalmus pectinirostris) hypoxia-inducible factor-1α (hif-1α) and analysis of its function in monocytes/macrophages. | hypoxia-inducible factor-1α (hif-1α) plays a critical role in immune and inflammatory responses and is important in controlling a variety of processes in monocytes and macrophages. however, very little information is available about the functions of hif-1α in fish monocytes/macrophages (mo/mф). in this study, the cdna sequence of the mudskipper (boleophthalmus pectinirostris) hif-1α gene (bphif-1α) was determined. sequence comparison and phylogenetic tree analysis showed that bphif-1α is cluster ... | 2017 | 28542591 |
| a cxcl ortholog from hippocampus abdominalis: molecular features and functional delineation as a pro-inflammatory chemokine. | chemokines are a family of chemotactic cytokines that regulate leukocyte migration. they are classified into four groups namely, cxc, cc, c and cx3c, based on the formation of a disulfide bridge. among these, cxc chemokines have been identified as the largest group of chemokines in humans. in this study, we identified and functionally characterized a homolog of cxc chemokine from the big-belly seahorse, hippocampus abdominalis, and designated it as shcxcl. the cdna of shcxcl composed of a 342-bp ... | 2017 | 28546023 |
| tissue specific expression profile of some immune related genes in labeo rohita to edwardsiella tarda infection. | rohu (labeo rohita), an indian major carp (imc) is an economically important aquaculture species in india. inspite of the technological advances, infectious diseases caused by viruses, bacteria and parasites have been a major limiting factor in the development and profitability of fish farms. at present, information regarding the immune status of the indian major carps is limited. this lack of knowledge is a major impediment for establishment of effective preventive measures against broad spectr ... | 2017 | 28549942 |
| the complete structure of the core oligosaccharide from edwardsiella tarda eib 202 lipopolysaccharide. | the chemical structure and genomics of the lipopolysaccharide (lps) core oligosaccharide of pathogenic edwardsiella tarda strain eib 202 were studied for the first time. the complete gene assignment for all lps core biosynthesis gene functions was acquired. the complete structure of core oligosaccharide was investigated by ¹h and (13)c nuclear magnetic resonance (nmr) spectroscopy, electrospray ionization mass spectrometry ms(n), and matrix-assisted laser-desorption/ionization time-of-flight mas ... | 2017 | 28561753 |
| glycolysis-related proteins are broad spectrum vaccine candidates against aquacultural pathogens. | reverse vaccinology (rv) has become a popular method for developing vaccines. although edwardsiella tarda is deemed to be an important fish pathogen, so far, no reports have used a genome-based approach to screen vaccine candidates against e. tarda. in the current study, protective antigens of e. tarda were screened using rv. large-scale cloning, expression and purification of potential candidates were carried out, and their immunoprotective potential was evaluated. a candidate fructose-bisphosp ... | 2017 | 28587729 |
| effect of crowding stress on the immune response in turbot (scophthalmus maximus) vaccinated with attenuated edwardsiella tarda. | the aim of this study was to evaluate the immune responses in turbot, scophthalmus maximus, treated with 1 × 10(7) cfu/ml attenuated edwardsiella tarda (0.1 ml/fish) under low density (ld; ∼5.25-5.13 kg/m(2), initial to final density), medium density (md; ∼10.41-13.95 kg/m(2)), and high density (hd; ∼20.53-30.77 kg/m(2)) conditions for 8 weeks. the results showed that there was a peak value in the percentage of sig+ (surface immunoglobulin-positive) cells in blood leucocytes (bl), spleen leucocy ... | 2017 | 28602742 |
| effect of feeding lipopolysaccharide as an immunostimulant on immune response and immune gene expression of labeo bata. | this study investigates the effects of dietary lipopolysaccharide (lps) as an immunostimulant on hematology, innate immunity, immune gene expression and protection against edwardsiella tarda on labeo bata. a basal diet supplemented with 0, 50, 100 and 150mg lps kg(-1)diet was fed to the four different groups for 30days. the haematological (total erythrocyte count, total leukocyte count, total serum protein, albumin and globulin), innate immune parameters (respiratory burst, serum lysozyme, myelo ... | 2017 | 28615127 |
| the first report of cathepsin a gene in a teleost (rock bream, oplegnathus fasciatus): an investigation of immune functions upon infection with several pathogens. | we isolated and characterised a cdna encoding the lysosomal protective protein (serine protease) cathepsin a (ctsa) from rock bream (oplegnathus fasciatus). the full-length rock bream ctsa (rbctsa) cdna (1814 bp) contains an open reading frame of 1419 bp that encodes 472 amino acids. alignment of multiple ctsa protein sequences revealed that the active site serine and histidine residues were well-conserved among the other ctsa sequences. rbctsa is highly expressed in the peripheral blood leukocy ... | 2017 | 28624491 |
| characterization of japanese flounder (paralichthys olivaceus) caspase1 involved in extracellular atp-mediated immune signaling in fish. | caspase1 is a member of inflammatory caspases that play important roles in the innate immune system. although several teleost caspase1 genes have been identified, their partner proteins and implication in extracellular atp-mediated immune signaling in fish are still very limited. here we identified and characterized a caspase1 gene, named jfcaspase1, from japanese flounder paralichthys olivaceus. jfcaspase1 mrna was constitutively expressed in all examined normal tissues with high expression in ... | 2017 | 28630015 |
| regulation of type iii secretion of translocon and effector proteins by the esab/esal/esam complex in edwardsiella tarda. | the type iii secretion system (t3ss) plays a crucial role in the pathogenesis of many gram-negative bacteria, including edwardsiella tarda, an important fish pathogen. within the e. tarda t3ss, there are three proteins (esab/esal/esam) that are homologous to proteins present in many other bacteria, including spic/ssal/ssam in salmonella, sepd/sepl/cesl in enteropathogenic escherichia coli (epec) and enterohemorrhagic e. coli (ehec), and yscb/yopn/sycn in yersinia esal was found to interact with ... | 2017 | 28630070 |
| lamp-1-chimeric dna vaccines enhance the antibody response in japanese flounder, paralichthys olivaceus. | dna vaccination is one method to protect farmed fish from viral and bacterial diseases. chimeric antigens encoded by dna vaccines have been shown to increase the resistance to viral diseases. here, we sequenced the gene encoding lysosome-associated membrane protein-1 from japanese flounder, paralichthys olivaceus, (jflamp-1) and assessed its use in a chimeric dna vaccine fused with the major capsule protein (mcp) from red seabream iridovirus (rsiv). jflamp-1 cdna has a length of 1248 bp encoding ... | 2017 | 28634010 |
| sequencing-based network analysis provides a core set of gene resource for understanding kidney immune response against edwardsiella tarda infection in japanese flounder. | marine organisms are under a frequent threat from various pathogens. edwardsiella tarda is one of the major fish pathogens infecting both cultured and wild fish species. it can also infect a variety of other vertebrates, including amphibians, reptiles, and mammals, and bacteremia caused by e. tarda can be fatal in humans. the kidney is the largest lymphoid organ in fish, and generating kidney transcriptomic information under different stresses is crucial for understanding molecular mechanisms un ... | 2017 | 28651821 |
| the immune adjuvant effects of flounder (paralichthys olivaceus) interleukin-6 on e. tarda subunit vaccine ompv. | interleukin-6 (il-6) as a pleiotropic cytokine was widely used as an effective adjuvant for vaccines in mammals. in this study, the immune adjuvant effects of two forms of flounder (paralichthys olivaceus) il-6, including recombinant il-6 (ril-6) and pcdna3.1-il-6 (pcil-6), were evaluated and comparatively analyzed on e. tarda subunit vaccine recombinant outer membrane protein v (rompv). the results showed that the relative percent survivals of flounder vaccinated with rompv plus ril-6 or pcil-6 ... | 2017 | 28678171 |
| a lactococcus lactis bfe920 feed vaccine expressing a fusion protein composed of the ompa and flgd antigens from edwardsiella tarda was significantly better at protecting olive flounder (paralichthys olivaceus) from edwardsiellosis than single antigen vaccines. | edwardsiellosis is a major fish disease that causes a significant economic damage in the aquaculture industry. here, we assessed vaccine efficacy after feeding oral vaccines to olive flounder (paralichthys olivaceus), either l. lactis bfe920 expressing edwardsiella tarda outer membrane protein a (ompa), flagellar hook protein d (flgd), or a fusion antigen of the two. feed vaccination was done twice with a one-week interval. fish were fed regular feed adsorbed with the vaccines. feed vaccination ... | 2017 | 28687358 |
| in vitro assessment of the antimicrobial activity of silver and zinc oxide nanoparticles against fish pathogens. | antibiotic resistance is a global issue that threatens public health. the excessive use of antibiotics contributes to this problem as the genes of antibiotic resistance can be transferred between the bacteria in humans, animals and aquatic organisms. metallic nanoparticles could serve as future substitutes for some conventional antibiotics because of their antimicrobial activity. the aim of this study was to evaluate the antimicrobial effects of silver and zinc oxide nanoparticles against major ... | 2017 | 28732514 |
| molecular, structural, and functional comparison of n lobe and c lobe of the transferrin from rock bream, oplegnathus fasciatus, with respect to its immune response. | the iron-withholding strategy of innate immunity is an effective antimicrobial defense mechanism that combats microbial infection by depriving microorganisms of fe(3+), which is important for their growth and propagation. transferrins (tfs) are a group of iron-binding proteins that exert their antimicrobial function through fe(3+) sequestration. the current study describes both structural and functional characteristics of a transferrin ortholog from rock bream oplegnathus fasciatus (rbtf). the r ... | 2017 | 28732766 |
| protective effects of cpg-odn 2007 administration against edwardsiella tarda infection in olive flounder (paralichthys olivaceus). | in this study, we investigated the immunostimulatory and protective effects of cpg motif oligonucleotides (cpg-odns) against edwardsiella tarda infection in olive flounder (paralichthys olivaceus). groups of fish injected with cpg-odns (1585, 1668, and 2007) or pbs (control) showed varying mortality rates in response to challenge with e. tarda. the survival rates of fish treated with cpg-odn 1668 and 2007, which belonged to the same class type b, were 45% and 60%, respectively, with cpg-odn 2007 ... | 2017 | 28732767 |
| manganese-superoxide dismutase (mnsod), a role player in seahorse (hippocampus abdominalis) antioxidant defense system and adaptive immune system. | manganese superoxide dismutase (mnsod) is a metaloenzyme that catalyzes dismutation of the hazardous superoxide radicals into less hazardous h2o2 and h2o. here, we identified a homolog of mnsod from big belly seahorse (hippocampus abdominalis; hamnsod) and characterized its structural and functional features. hamnsod transcript possessed an open reading frame (orf) of 672 bp which codes for a peptide of 223 amino acids. pairwise alignment showed that hamnsod shared highest identity with rock bre ... | 2017 | 28743628 |
| antimicrobial property of lemongrass (cymbopogon citratus) oil against pathogenic bacteria isolated from pet turtles. | the usage of essential oils as antimicrobial agents is gaining attention. besides, pet turtles were known to harbor a range of pathogenic bacteria while the turtle keeping is a growing trend worldwide.the current study examined the antimicrobial activity of lemon grass oil (lgo) against seven species of gram negative bacteria namely; aeromonas hydrophila, a. caviae, citrobacter freundii, salmonella enterica, edwardsiella tarda, pseudomonas aeruginosa, and proteus mirabilis isolated from three po ... | 2017 | 28747972 |
| production of rnase iii-knockout, auxotrophic edwardsiella tarda mutant for delivery of long double-stranded rna and evaluation of its immunostimulatory potential. | the artificially synthesized polyinosinic-polycytidylic acid (poly ic) has been widely used to induce type i ifn responses in various vertebrates including fish. however, as poly ic is too expensive to use in aquaculture, the development of another economical long dsrna producing method is needed to practically use long dsrnas in aquaculture farms for the control of infectious diseases. in the present study, to produce long dsrnas economically, we developed a novel long dsrna production system b ... | 2017 | 28756288 |
| edwardsiella tarda-induced mirnas in a teleost host: global profile and role in bacterial infection as revealed by integrative mirna-mrna analysis. | 2017 | 28762875 | |
| thymosin β4 is involved in the antimicrobial immune response of golden pompano, trachinotus ovatus. | thymosin beta belongs to the thymosin family, which consists of a series of highly conserved peptides involved in various biological processes. in teleosts, understanding of the immunological functions of thymosin beta is limited, particularly in vivo, which is essentially unknown. in the current study, we cloned and identified thymosin beta 4 from the teleost fish golden pompano (trachinotus ovatus), which we have named trotβ4. we investigated the expression patterns and functions of trotβ4 in ... | 2017 | 28803959 |
| molecular insights of two stat1 variants from rock bream (oplegnathus fasciatus) and their transcriptional regulation in response to pathogenic stress, interleukin-10, and tissue injury. | signal transducers and activators of transcription 1 (stat1) is critically involved in mediating cytokine-driven signaling, and triggers the transcription of target genes to activate cellular functions. although the structural and functional aspects of stat members have been well described in mammals, only limited information is available for the stat genes in teleost fishes. in the present study, two variants of stat1 genes (rbstat1 and rbstat1l) were identified from rock bream and characterize ... | 2017 | 28818616 |
| proteomic analysis of macrophage in response to edwardsiella tarda-infection. | edwardsiella tarda is an important facultative intracellular pathogen infecting a wide range of host from fish to humans. this bacterium could survive and replicate in macrophages as an escape mechanism from the host defense. e. tarda -macrophage interaction is vital in determining the outcome of edwardsiellasis. to fully elucidate the pathogenesis of e. tarda, the differential proteomes of raw264.7 cells in response to e. tarda-infection, were analyzed at different time points with two-dimensio ... | 2017 | 28826764 |
| antibiotic and heavy metal resistance of aeromonas hydrophila and edwardsiella tarda isolated from red hybrid tilapia (oreochromis spp.) coinfected with motile aeromonas septicemia and edwardsiellosis. | the aim of this study is to identify antibiogram and heavy metal resistance pattern of aeromonas hydrophila and edwardsiella tarda isolated from red hybrid tilapia (oreochromis spp.) coinfected with motile aeromonas septicemia and edwardsiellosis in four commercial fish farms. | 2017 | 28831226 |
| identification and molecular profiling of dc-sign-like from big belly seahorse (hippocampus abdominalis) inferring its potential relevancy in host immunity. | dendritic-cell-specific icam-3-grabbing non-integrin (dc-sign) is a c-type lectin that functions as a pattern recognition receptor by recognizing pathogen-associated molecular patterns (pamps). it is also involved in various events of the dendritic cell (dc) life cycle, such as dc migration, antigen capture and presentation, and t cell priming. in this study, a dc-sign-like gene from the big belly seahorse hippocampus abdominalis (designated as shdcs-like) was identified and molecularly characte ... | 2017 | 28867209 |
| possible probiotic lactic acid bacteria isolated from oysters (crassostrea gigas). | we attempted to isolate lactic acid bacteria (lab) from the marine oyster (crassostrea gigas) and selected several environmental stress-resistant isolates for the development of a future probiotic adjuvant for marine aquaculture. twenty-six presumptive lab isolates were extracted from oysters and screened (by an agar diffusion assay) for antimicrobial activity toward various pathogens: vibrio parahaemolyticus, streptococcus iniae, and edwardsiella tarda. eight isolates had an antibacterial activ ... | 2017 | 28875385 |
| infections of edwardsiella tarda among brook trout in quebec. | acute bacterial septicemia is commonly diagnosed in brook trout salvelinus fontinalis of quebec, canada. the agents most commonly isolated include aeromonas salmonicida (furunculosis), aeromonas hydrophila (motile aeromonad septicemia), and pseudomonas species. septicemia in brook trout caused by the gram-negative bacterium edwardsiella tarda was diagnosed for the first time in the province of quebec from two different fish farms producing stock for fee fishing establishments. affected fish disp ... | 2000 | 28880779 |
| molecular cloning, biochemical characterization, and expression analysis of two glutathione s-transferase paralogs from the big-belly seahorse (hippocampus abdominalis). | glutathione s-transferases (gsts, ec 2.5.1.18) are important phase ii detoxifying enzymes that catalyze hydrophobic, electrophilic xenobiotic substance with the conjugation of reduced glutathione (gsh). in this study, gstμ and gstρ paralogs of gst in the big belly seahorse (hippocampus abdominalis; hagstρ, hagstμ) were biochemically, molecularly, functionally characterized to determine their detoxification range and protective capacities upon different pathogenic stresses. hagstρ and hagstμ are ... | 2017 | 28882453 |
| modification of the plasma complement protein profile by exogenous estrogens is indicative of a compromised immune competence in marine medaka (oryzias melastigma). | growing evidence suggests that the immune system of teleost is vulnerable to xenoestrogens, which are ubiquitous in the marine environment. this study detected and identified the major circulatory immune proteins deregulated by 17α-ethinylestradiol (ee2), which may be linked to fish susceptibility to pathogens in the marine medaka, oryzias melastigma. fish immune competence was determined using a host resistance assay to pathogenic bacteria edwardsiella tarda. females were consistently more susc ... | 2017 | 28882797 |
| transcriptome profiling based on protein-protein interaction networks provides a core set of genes for understanding blood immune response mechanisms against edwardsiella tarda infection in japanese flounder (paralichthys olivaceus). | marine organisms are commonly under threat from various pathogens. edwardsiella tarda is one of the fish pathogens that can infect both cultured and wild fish species. e. tarda can also infect other vertebrates, including amphibians, reptiles, and mammals. bacteremia caused by e. tarda can be a fatal disease in humans. blood acts as a pipeline for the fish immune system. generating blood transcriptomic resources from fish challenged by e. tarda is crucial for understanding molecular mechanisms u ... | 2018 | 28923591 |
| intracellular trafficking pathways of edwardsiella tarda: from clathrin- and caveolin-mediated endocytosis to endosome and lysosome. | edwardsiella tarda is a gram-negative bacterium that can infect a broad range of hosts including humans and fish. accumulating evidences have indicated that e. tarda is able to survive and replicate in host phagocytes. however, the pathways involved in the intracellular infection of e. tarda are unclear. in this study, we examined the entry and endocytic trafficking of e. tarda in the mouse macrophage cell line raw264.7. we found that e. tarda entered raw264.7 and multiplied intracellularly in a ... | 2017 | 28932708 |
| comparative phenotypic and genotypic analysis of edwardsiella isolates from different hosts and geographic origins, with emphasis on isolates formerly classified as e. tarda, and evaluation of diagnostic methods. | edwardsiella spp. are responsible for significant losses in important wild and cultured fish species worldwide. recent phylogenomic investigations have determined that bacteria historically classified as edwardsiella tarda actually represent three genetically distinct yet phenotypically ambiguous taxa with various degrees of pathogenicity in different hosts. previous recognition of these taxa was hampered by the lack of a distinguishing phenotypic character. commercial test panel configurations ... | 2017 | 28978684 |
| novel ifnγ homologue identified in nile tilapia (oreochromis niloticus) links with immune response in gills under different stimuli. | interferon gamma (ifn-γ) has important roles in both innate and adaptive immune responses. this cytokine plays a very important role in defining th1 immune response in all vertebrates. in the present study, we identified and isolated for the first time the gene coding for nile tilapia (oreochromis niloticus) ifnγ from spleen lymphocytes. the isolated tilapia ifnγ has between 24 and 62% of amino acid identity as compared to reported sequences for other teleost fishes. it has close phylogenetic re ... | 2017 | 29017941 |
| molecular cloning and functional characterization of peptidoglycan recognition protein ompgrp-l2 from the rainbow trout, oncorhynchus mykiss. | peptidoglycan recognition proteins (pgrps), a group of pattern recognition receptors (prrs), are innate immune molecules that are structurally conserved through evolution in both invertebrate and vertebrate animals. in teleost fish, several pgrps have been characterized recently. they have both amidase activity and bactericidal activity and are involved in indirectly killing bacteria and regulating multiple signaling pathways. however, the knowledge of functional similarity and divergence betwee ... | 2017 | 29042012 |
| maternal peripartum septic shock caused by intrauterine infection with edwardsiella tarda: a case report and review of the literature. | edwardsiella tarda (e. tarda) is a rare pathogen in humans, especially during the peripartum period. only a few cases of fatal neonatal infection with e. tarda have been reported. herein, we describe a case of maternal septicemia caused by e. tarda following peripartum chorioamnionitis. the mother developed septic shock, disseminated intravascular coagulation and a post-cesarean wound hematoma with abscess. her condition improved with multidisciplinary therapy including blood transfusion, antimi ... | 2017 | 29094482 |
| effect of microalgae on intestinal inflammation triggered by soybean meal and bacterial infection in zebrafish. | soybean meal has been used in many commercial diets for farm fish; despite this component inducing intestinal inflammation. on the other hand, microalgae have increasingly been used as dietary supplements in fish feed. nevertheless, the vast quantity of microalgae species means that many remain under- or unstudied, thus limiting wide scale commercial application. in this work, we evaluated the effects to zebrafish (danio rerio) of including tetraselmis sp (ts); phaeodactylum tricornutum (pt); ch ... | 2017 | 29117213 |
| identification and expression of complement component c8α, c8β and c8γ gene in half-smooth tongue sole (cynoglossus semilaevis) and c8α recombinant protein antibacterial activity analysis. | complement component c8, which mediates membrane attack complex formation and bacterial lysis, plays important roles in the complement system. the cdna sequences of the c8α, c8β and c8γ genes were cloned from half-smooth tongue sole (cynoglossus semilaevis). full-length cdna of csc8α (c8α of c. semilaevis), csc8β and csc8γ was 1990, 2219 and 886 bp, respectively, which contained open reading frames of 1797, 1749 and 666 bp, encoding 598, 582 and 221 amino acids, respectively. the deduced protein ... | 2018 | 29146450 |
| genetic studies to re-affiliate edwardsiella tarda fish isolates to edwardsiella piscicida and edwardsiella anguillarum species. | until 2012, the genus edwardsiella was composed by three species edwardsiella tarda, edwardsiella hoshinae and edwardsiella ictaluri. in 2013, edwardsiella piscicida, compiling fish pathogenic strains previously identified as e. tarda was described, and more recently a new species isolated from diseased eel was reported, namely edwardsiella anguillarum. the incorporation of these species into the genus makes necessary a revision of the taxonomic position of the isolates previously identified as ... | 2017 | 29150173 |
| mudskipper (boleophthalmus pectinirostris) hepcidin-1 and hepcidin-2 present different gene expression profile and antibacterial activity and possess distinct protective effect against edwardsiella tarda infection. | hepcidins are small cysteine-rich antimicrobial peptides that play an important role in fish immunity against pathogens. most fish species have two or more hepcidin homologs that have distinct functions. this study investigated the immune functions of mudskipper (boleophthalmus pectinirostris) hepcidin-1 (bphep-1) and hepcidin-2 (bphep-2) in vitro and in vivo. upon infection with edwardsiella tarda, the expression of bphep-1 and bphep-2 mrna in immune tissues was significantly upregulated, but t ... | 2017 | 29151250 |
| preparation, characterization, and antimicrobial properties of chitosan-silver nanocomposites films against fish pathogenic bacteria and fungi. | development of nanostructured films using natural polymers and metals has become a considerable interest in various biomedical applications. objective of the present study was to develop silver nano particles (agnps) embedded chitosan films with antimicrobial properties. based on the ag content, two types of chitosan silver nano films, named as cagnfs-12 (12 mm) and cagnfs-52 (52 mm) were prepared and characterized. field emission scanning electron microscope (fe-sem) images of two cagnfs showed ... | 2017 | 29151644 |
| insights into the multifunctional role of natural killer enhancing factor-a (nkef-a/prx1) in big-belly seahorse (hippocampus abdominalis): dna protection, insulin reduction, h2o2 scavenging, and immune modulation activity. | natural killer enhancing factor a (nkef-a), also known as peroxiredoxin 1 (prx1), is a well-known antioxidant involved in innate immunity. although nkef-a/prx1 has been studied in different fish species, the present study broadens the knowledge of nkef-a gene in terms of molecular structure, function, and immune responses in fish species. hippocampus abdominalis nkef-a (hankef-a) cdna encoded a putative protein of 198 amino acids containing a thioredoxin_2 domain, vcp motifs, and three conserved ... | 2018 | 29155258 |
| identification, expression analysis, and antibacterial activity of nk-lysin from common carp cyprinus carpio. | natural killer lysin (nk-lysin), produced by cytotoxic t lymphocytes and natural killer cells, is a cationic antimicrobial peptide that has a broad antimicrobial spectrum, including bacteria, viruses, and parasites. nevertheless, the implication of nk-lysin in the protection against bacterial infection is not aware in common carp. in this study, six different nk-lysin genes (nkl1, nkl2, nkl3, nkl4, nkl5 and nkl6) were identified in the common carp genome. each of the mature peptides of common ca ... | 2018 | 29162543 |
| transcriptome profiling provides gene resources for understanding gill immune responses in japanese flounder (paralichthys olivaceus) challenged with edwardsiella tarda. | marine organisms are commonly under threats from various pathogens. edwardsiella tarda is one of the fish pathogens that seriously infect cultured and wild fish species. bacteremia caused by e. tarda can be a fatal disease in humans. fish gill is a mucosa-associated lymphoid tissue that directly contacted with sea water. generating gill transcriptomic resources that challenged by e. tarda is crucial for understanding the molecular mechanisms underlying gill immune responses. in this study, we pe ... | 2018 | 29175442 |
| to17: a teleost antimicrobial peptide that induces degradation of bacterial nucleic acids and inhibits bacterial infection in red drum, sciaenops ocellatus. | tissue factor pathway inhibitor (tfpi)-1 is well known for its role as an inhibitor of blood coagulation. several studies have demonstrated that the c-terminal peptides of tfpi-1 are active against a broad spectrum of microorganisms. in a previous study, we found that to17 (with 17 amino acids), a tfpi-1 c-terminal peptide from red drum (sciaenops ocellatus), was active against edwardsiella tarda. in the present study, we investigated further the antimicrobial spectrum, action mode, as well as t ... | 2018 | 29183811 |
| edwardsiella tarda bacteremia with psoas and epidural abscess as a food-borne infection: a case report and literature review. | edwardsiella tarda is commonly isolated from aquatic environments and a variety of animals. we present the first case of e. tarda bacteremia with psoas and epidural abscess. the patient was a 65-year-old woman with recurrent gastric cancer who had frequently consumed raw fish and grilled eel. she was successfully treated with antimicrobials and surgery. we also review reports published in english regarding e. tarda bacteremia in japan and the experience at our hospital. on the basis of this revi ... | 2017 | 29225255 |
| functional characterization of a short peptidoglycan recognition protein from chinese giant salamander (andrias davidianus). | peptidoglycan (pgn) recognition proteins (pgrps) are important pattern recognition receptors (prrs) involved in immune defense against bacterial infections. in this study, a short pgrp (termed adpgrp-s1) was cloned and functionally characterized from chinese giant salamander (andrias davidianus), the largest extant urodela amphibian species. adpgrp-s1 was 184 aa in length and shared 38.7%-54.9% sequence identities with other vertebrates' short pgrps. it contained one typical pgrp domain at the c ... | 2017 | 29245904 |
| edwardsiella tarda tunes tricarboxylic acid cycle to evade complement-mediated killing. | evasion of complement-mediated killing is a common phenotype for many different types of pathogens, but the mechanism is still poorly understood. most of the clinic isolates of edwardsiella tarda, an important pathogen infecting both of human and fish, are commonly found serum-resistant. to explore the potential mechanisms, we applied gas chromatography-mass spectrometry (gc-ms)-based metabolomics approaches to profile the metabolomes of e. tarda eib202 in the presence or absence of serum stress ... | 2017 | 29270172 |
| construction, immune protection and innate immune response of shuffled polyvalent ompas vaccines. | our previous studies demonstrated that molecular breeding via dna shuffling directs the evolution of polyvalent vaccines with desired traits, which leads to generation of polyvalent ompa vaccines using vibrio alginolyticus va0764 primers. here, we replaced va0764 primers with edwardsiella tarda ompa primers to generate new polyvalent ompa vaccines by dna shuffling of the same five ompa genes from four species of bacteria e. tarda, v. parahaemolyticus, v. alginolyticus and escherichia coli. we id ... | 2017 | 29289655 |
| recombinant hsp33 and ompc protein can serve as promising divalent vaccine with protection against vibrio anguillarum and edwardsiella tarda in flounder (paralichthys olivaceus). | vibrio anguillarum and edwardsiella tarda are severe aquaculture pathogens shared similar epidemiological characteristics and susceptible to flounder (paralichthys olivaceus). in our previous studies, recombinant(r) protein heat shock protein 33 (rhsp33) from v. anguillarum and outer membrane protein c (rompc) from e. tarda were proved to have protection against v. anguillarum and e. tarda, respectively. in this paper, the cross protection of rhsp33 against e. tarda and rompc against v. anguilla ... | 2018 | 29309834 |