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[the rheumatic complications of pseudotuberculosis in a newly arrived population in siberia]. 19911887407
[the comparative effectiveness of serological and microbiological studies in the etiological diagnosis of reactive arthritis].the information content of serological and microbiological studies was studied and compared in 72 patients suffering from enterogenous reactive arthritis. a high level of antibodies to intestinal yersinia was detected in 11.1% of the patients, positive coproculture in 8.3%. only one patient demonstrated at a time a high level of antibodies and isolation of intestinal yersinia coproculture. the causative agent could usually be isolated in patients with the symptoms of enterocolitis. it is assumed ...19911887408
[pathology of the gastrointestinal tract in patients with reactive arthritis and its possible role in the pathogenesis of the disease]. 19911887410
factors promoting acute and chronic diseases caused by yersiniae.the experimental system constructed with the medically significant yersiniae provides a powerful basic model for comparative study of factors required for expression of acute versus chronic disease. the system exploits the close genetic similarity between yersinia pestis, the etiological agent of bubonic plague, and enteropathogenic yersinia pseudotuberculosis and yersinia enterocolitica. y. pestis possesses three plasmids, of which one, shared by the enteropathogenic species, mediates a number ...19911889045
cytogenesis in the monkey retina.time of cell origin in the retina of the rhesus monkey (macaca mulatta) was studied by plotting the number of heavily radiolabeled nuclei in autoradiograms prepared from 2- to 6-month-old animals, each of which was exposed to a pulse of 3h-thymidine (3h-tdr) on a single embryonic (e) or postnatal (p) day. cell birth in the monkey retina begins just after e27, and approximately 96% of cells are generated by e120. the remaining cells are produced during the last (approximately 45) prenatal days an ...19911894769
identification and cloning of a fur regulatory gene in yersinia pestis.yersinia pestis is one of many microorganisms responding to environmental iron concentrations by regulating the synthesis of proteins and an iron transport system(s). in a number of bacteria, expression of iron uptake systems and other virulence determinants is controlled by the fur regulatory protein. dna hybridization analysis revealed that both pigmented and nonpigmented cells of y. pestis possess a dna locus homologous to the escherichia coli fur gene. introduction of a fur-regulated beta-ga ...19911898928
bacterial entry into eukaryotic cells. 19911905978
[study of the dependence of gene expression of plague pathogen plasmid 65md on the host bacterium genome].the conjugative cointegrate containing yersinia pestis 65 md plasmid coding for the production of traction i antigen and mouse toxin has been transferred into yersinia pseudotuberculosis, yersinia enterocolitica and escherichia coli cells. analysis of the transconjugants obtained has confirmed the connection of the genetical determinants for the mentioned bacterial products with the 65 md plasmid. the similar level of fra and tox-genes expression has been found in yersinia cells while fraction i ...19911906986
[antibacterial and therapeutic effectiveness of a pectin from sea grass zostera].the kinetics of the antibacterial activity of zosterin, a polysaccharide preparation of a sea grass belonging to zoster, was studied. by its chemical structure zosterin is a low ++methoxylated pectin. in vitro the preparation markedly inhibited the growth of ++gram-negative and ++gram-positive organisms: s. aureus, e. coli, y. pseudotuberculosis, s. typhimurium and ps. aeruginosa. on a model of experimental pseudotuberculosis++ infection caused by oral contamination of mice f1 (cba x c57b1) with ...19911909858
[study by nmr and circular dichroism of synthetic "zinc finger" peptide of viral gag protein from hiv-2].we report the synthesis in solid phase of an 18-amino acid peptide that contains the cysteine-rich region of the structural gag protein of hiv-2. the characterization of this fragment and of its interaction with zn2+ has been made by one- (1 d) and two-dimensional (2d) nmr techniques and by circular dichroism. our results suggest that in aqueous solution the complexation produces a significant perturbation in the conformation of this peptide.19911913257
muscarinic-receptor functioning in tracheas from normal and ovalbumin-sensitive guinea pigs.responses to bilateral vagal nerve stimulation, to field stimulation, and to exogenous methacholine and histamine were compared in tracheas isolated from (a) saline injected (i.p.) and saline-aerosol exposed guinea pigs (control), (b) ovalbumin-sensitized and saline-aerosol exposed (sensitized) guinea pigs, and (c) ovalbumin-sensitized and 2% ovalbumin-aerosol exposed (challenged) guinea pigs. tracheal pressor responses (cmh2o; 1 cmh2o = 98.1 pa) to nerve and field stimulation, and maximal respo ...19911913333
fusarium oxysporum: status in bioethanol production.fermentation of lignocellulosic materials to ethanol and other solvents provides an alternative way of treating wastes and producing chemical feedstocks and fuel additives. considerable efforts have been made in past 10 years to improve the process based on lignocellulosic biomass and hydrolysate that contains a complex mixture of sugars, decomposition products of sugars, and sometimes the inhibitory levels of soluble lignin. despite the relative abundance of d-xylose in crop and forest residues ...19911913845
[approach to evaluation of the indicators of yersinia infections in reactive and rheumatoid arthritis].a total of 140 patients with reactive arthritis due to yersinia infection were under observation for 2-10 years. a group of 104 patients with erosive rheumatoid arthritis served as control for analysis of the results of bacteriological and serological studies. the bacteriological analysis of the feces, urine, smears of the feces and blood were made repeatedly. as established the articular syndrome in reactive arthrosis has its specific features depending on the nature of the pathological process ...19911925260
yersinia pseudotuberculosis in a persian cat. 19911926722
wild animals as the source of infection with yersinia pseudotuberculosis in shimane prefecture, japan. 19911935073
the v antigen of yersiniae: an overview. 19911935079
renal insufficiency in mice experimentally infected with yersinia pseudotuberculosis. 19911935080
experimental infection of mice with yersinia strains bearing or not bearing the virulence-associated plasmid. 19911935082
adhesion of yersiniae to rabbit intestinal constituents: role of outer membrane protein yada and modulation by intestinal mucus. 19911935087
chromosomal marker for the 'high pathogenicity' phenotype in yersinia. 19911935090
molecular biology of yersinia pseudotuberculosis large plasmid pvm82. 19911935092
plasmid-mediated characteristics of yersinia pseudotuberculosis strains isolated in italy from humans, cattle and swine. 19911935093
preparation of monoclonal antibody to v antigen from yersinia pestis. 19911935094
cases of yersinia pseudotuberculosis infection having diagnostic criteria of kawasaki disease. 19911935106
several aspects of yersinia pseudotuberculosis infection in children. 19911935107
renal complications of yersinia pseudotuberculosis infection in children. 19911935108
yersinia pseudotuberculosis infection in the mountain area. 19911935109
yersinia pseudotuberculosis infection in children due to untreated drinking water. 19911935113
comparison of plasmid dna among different serogroups of yersinia pseudotuberculosis. 19911935117
intracellular targeting of the yersinia yope cytotoxin in mammalian cells induces actin microfilament disruption.pathogenic yersinia spp., including the etiological agent of plague, y. pestis, all carry a common plasmid that encodes a number of essential virulence determinants, the yop proteins. one of these, yope, has been shown to be involved in the obstruction of the primary host defense by a molecular mechanism leading to inhibition of phagocytosis (r. rosqvist, a. forsberg, m. rimpiläinen, t. bergman, and h. wolf-watz, mol. microbiol. 4:657-667, 1990). although the yop proteins are secreted into the c ...19911937815
image cytometry of progesterone receptor expression during the cell cycle in the mcf-7 cell line.progesterone receptors (pr) appear to be distributed in a heterogeneous way in mammary tumor cells. the study presented here was designed to examine if heterogeneity of pr expression is cell-cycle dependent. immunofluorescence techniques were used to label pr on the mcf-7 human breast cancer cell line and image cytometry was used to analyze the pr expression during g0 (ki-67 antigen-negative cells), g1, s, and g2/m cell-cycle phases. a second pr, brdu, and dna analysis was performed to study pr ...19911940323
[yersiniosis in a general hospital in the basque country (1984-1989). clinical and epidemiological aspects].the features of the diseases caused by yersinia have not been properly evaluated in the basque country. the present study was performed to assess the incidence and consequences of this infection.19911943291
purification and partial characterization of a soluble hemagglutinin from yersinia pseudotuberculosis.a soluble hemagglutinin (ha) produced by yersinia pseudotuberculosis strain inoue, serotype 5b, was purified by ammonium sulfate precipitation, gel filtration on sepharose cl-6b and high performance liquid chromatography on a deae-5pw anion-exchange column. the purified ha was a 14.5 kda protein with an isoelectric point of 4.5. amino acid analysis indicated that the ha consisted of 133 residues, corresponding to the molecular weight of 14,100. the amino acid sequence of n-terminal 38 amino acid ...19911943846
[postcoital uterine microbe colonization and endometritis in the mare].in the mare, natural breeding is associated with bacterial contamination of the reproductive tract. the purpose of this study was to examine postcoital bacterial contamination and the resulting inflammatory response of the uterus. uterine swabs for bacteriological and cytological examination were obtained from 80 mares. each mare was sampled once between 4 and 69 hours postbreeding. in mares which did not conceive, sampling was repeated at the following estrus. the findings were compared with th ...19911948985
a review of 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced changes in immunocompetence: 1991 update.2,3,7,8-tetrachlorodibenzo-p-dioxin, more popularly called dioxin or tcdd and referred to in this review as 2,3,7,8-tcdd, is considered the prototype of the polychlorinated dibenzo-p-dioxins (pcdd). the pcdd are true contaminants and are formed primarily as byproducts in the manufacture of materials requiring the use of chlorinated phenols and during the combustion of chlorinated chemical products. from an environmental perspective, the pcdd have been most closely associated with the use of a nu ...19911949050
[the immunoenzyme analysis of ceruloplasmin].a highly specific and sensitive enzyme immunoassay (eia) system, suitable for the qualitative analysis of ceruloplasmin, has been developed. the possibility of its use for the examination of children with mononucleosis and pseudotuberculosis has been studied. an increase in the concentration of ceruloplasmin has been more pronounced in infectious mononucleosis (0.506 +/- 0.026 g/l) and pseudotuberculosis (0.421 +/- 0.157 g/l). the results of eia coincided with the data obtained by radial immunod ...19911950269
[the detection of human pseudotuberculosis cases in volyn province from 1975 to 1989]. 19911950294
[cytological examination of urethral and endocervical smears in the diagnosis of infections].cytologic studies of smears from 130 women suffering from various diseases, including 61 pregnant patients with gestoses, have shown structural changes of the cells in the smears from the endocervix and urethra, that permitted the diagnosis of mycoplasma infection in 43.8% of patients, chlamydia infection, 52.3%, and herpes simplex in 72.3% of patients; in 63.9% of patients these infections were combined. cytologic studies may be useful in the tentative diagnosis of urogenital infections, includ ...19911951977
failure of beta-lactam antibiotics and marked efficacy of fluoroquinolones in treatment of murine yersinia pseudotuberculosis infection.the treatment of yersiniosis by beta-lactams is questionable considering the proven failure of newer beta-lactams for treating murine yersinia enterocolitica infection. another modality of experimental treatment was performed with a virulent strain of y. pseudotuberculosis (nonproducer of beta-lactamase) highly susceptible (in terms of mics) to amoxicillin, cefotaxime, ceftriaxone, imipenem, doxycycline, gentamicin, and ofloxacin. the in vivo comparative efficacy of these drugs was evaluated in ...19911952849
[bacterial ileocecitis, a "new" disease].786 patients with suspected acute appendicitis or appendiceal mass were examined by ultrasonography to distinguish appendicitis from bacterial enteritis. 533 of these patients were described before. in 91 (11.6%) ultrasonography revealed the characteristic picture associated with bacterial enteritis of the ileocaecal region-enlarged mesenteric lymph nodes and mural thickening of the terminal ileum and caecum--but no image of an inflamed appendix. in 64 of these a bacterial infection was confirme ...19911956444
[the biological action of yersinia pseudotuberculosis endotoxin].experimental studies on guinea pigs have shown that y. pseudotuberculosis lipopolysaccharide is capable of inducing endotoxinemia accompanied by the development of the thrombohemorrhagic syndrome. in cases of pseudotuberculosis the importance of the increased synthesis of prostaglandins and cyclic nucleotides with the prevalence of pgf2 alpha and cgmp in the genesis of toxico-allergic manifestations of pathologic processes has been established. the pathomorphological picture of pseudotuberculosi ...19901963723
[the protective properties of a porin from the outer membrane of yersinia pseudotuberculosis].the protective properties of the species-specific pore-forming polypeptide of y. pseudotuberculosis outer membrane (porin) were studied. the study showed that 80-90% of mice immunized with porin survived after challenge with 10-30 ld50 of y. pseudotuberculosis, serovars 1 and 3. the ld50 of the preparation exceeded its ed50 more than 100-fold. immunization made in two injections was more effective than immunization in one injection. the immunization of the animals with porin led to an increase i ...19901965874
[reactive arthritis following yersinia pseudotuberculosis infection].a patient developed reactive arthritis due to yersinia pseudotuberculosis. five months after the primary infection, the patient still suffered from severe oligoarthritis. the administration of steroids and sulphasalazine had very little effect upon the development of the disease.19901968686
a cloned chromosomal dna fragment which differentiates yersinia pestis from yersinia pseudotuberculosis and yersinia enterocolitica.chromosomal dna from reference yersinia strains was digested individually with 9 restriction endonucleases. dna fragments were separated and analyzed by electrophoresis through agarose gels. the clearest fragment patterns were obtained when ecori was employed. the y. pestis fragment pattern obtained after the use of this enzyme showed the presence of a unique dna fragment with molecular mass 1400 bp. this dna fragment was cloned, purified, labeled with 32p and then used to probe ecori digests of ...19901980253
application of the polymerase chain reaction and immunofluorescence techniques to the detection of bacteria in yersinia-triggered reactive arthritis.leukocytes in synovial fluid and peripheral blood samples from patients with yersinia-triggered reactive arthritis were analyzed after dna amplification using the polymerase chain reaction. the primers applied were specific for the virulence plasmid-coded 1cre genes of yersinia enterocolitica o:3 and yersinia pseudotuberculosis iii. no yersinia dna was observed within the synovial fluid cells or peripheral blood cells by polymerase chain reaction techniques. however, yersinia antigens were detec ...19911984781
combined use of released proteins and lipopolysaccharide in enzyme-linked immunosorbent assay for serologic screening of yersinia infections.an elisa for the screening of serum antibodies to yersinia species was developed using plasmid-encoded released proteins of yersinia enterocolitica o:8 and lipopolysaccharide of y. enterocolitica o:3 as a combined antigen. of 43 sera from patients infected with one of six different yersinia serotypes, 40 (93%) were positive in this assay. when tested using six serotype-specific elisas with the corresponding yersinia bacteria as antigens, 38 (88%) were positive. this screening elisa detects antib ...19911988526
[yersinia pseudotuberculosis in new world monkeys].it is reported on four cases of pseudotuberculosis in 2 saimiri sciureus, 1 callithrix jacchus/penicillata hybrid and 1 s. oedipus. the animals came from two different groups, one being infected with y. pseudotuberculosis serotype i, the other with y. pseudotuberculosis serotype ii. all cases showed intestinal infection of thermophile campylobacter. remarkable were severe haemorrhagic components and the distinct rhs-proliferation, especially in the mesenteric lymphatic nodes.19912015031
role of the yersinia outer membrane protein yada in adhesion to rabbit intestinal tissue and rabbit intestinal brush border membrane vesicles.the yersinia virulence plasmid confers on strains of yersinia pseudotuberculosis and y. enterocolitica an adhesive potential superior to the one encoded by the chromosome alone. we have evaluated the role of the plasmid-encoded outer membrane protein yada (formerly called yopl) in adhesion. insertional inactivation of the yada gene (formerly called yopa), which encodes yada, led to a reduction in the capacity of plasmid-carrying strains of y. pseudotuberculosis 0:iii and y. enterocolitica 0:9 to ...19912018636
control of pseudotuberculosis in zoos. 19912021043
risk of yersinia infection among butchers.the purpose of this study was to evaluate the risk of yersinia infection among butchers. serum samples were collected from 146 abattoir workers, stratified into 3 groups according to exposure to swine throats and intestines. 100 healthy blood donors were used as controls. antibodies against yersinia enterocolitica o:3 and o:9 and y. pseudotuberculosis i and iii were measured using elisa. symptoms associated with yersiniosis were recorded in a questionnaire. antibodies against y. enterocolitica o ...19912028228
pseudotuberculosis at the zoological society of london (1981 to 1987) 19912028568
acute tubulointerstitial nephritis associated with yersinia pseudotuberculosis infection.two siblings infected with yersinia pseudotuberculosis suffered from acute renal failure about 2 weeks after the onset of the disease. renal histology in both siblings showed acute tubulointerstitial nephritis. yersinia pseudotuberculosis type vb was isolated from feces of one of them, antibodies to yersinia pseudotuberculosis type vb in their sera were elevated. the results of the present study suggest that acute renal failure complicating infections with yersinia pseudotuberculosis is due to a ...19912032394
zoonoses and other findings in hedgehogs (erinaceus europaeus): a survey of mortality and review of the literature.a survey of mortality in hedgehogs (erinaceus europaeus) was carried out between july 1976 and november 1986. most were from norfolk. of the 74 examined, 35 (47.3 per cent) were road casualties, one of which yielded salmonella typhimurium phage type (pt) 104. of the remaining 39, 13 (33.3 per cent) had salmonellosis due to s enteritidis pt 11. this organism, which appears to be common and widespread in hedgehogs in england was found in 10 separate incidents. the only other zoonosis was ringworm ...19912035215
[yersinia and campylobacter food poisoning]. 19912038688
efficacy and safety of recombinant human erythropoietin to prevent the anaemias of prematurity. european randomized multicenter trial. 19912040194
meiotic drive of t haplotypes: chromosome segregation in mice with tertiary trisomy.the properties of the t haplotypes, specific mutant states of the proximal region of chromosomes 17 in the house mouse, are of continuing interest. one such property is increased transmission of the t haplotype by heterozygous t/+ males to offspring. using the reciprocal translocation t(16;17)43h we have constructed males with tertiary trisomy of chromosome 17 (+t43/+ +/rb7+) carrying the robertsonian translocation rb(16.17)7bnr. only the progeny of these males which had inherited either t43/+ o ...19912040454
yersinia species isolated from sheep with enterocolitis.in 40 submissions to the regional veterinary laboratory (rvl) wagga wagga from sheep in southern new south wales from 1981 to 1989, 53 isolates of yersinia sp were recovered from 45 sheep in 37 flocks. of 53 isolates, 26 were identified as y. pseudotuberculosis, 20 as y. enterocolitica, 5 as y. intermedia and 2 as y. frederiksenii. twelve isolates of y. pseudotuberculosis tested in the slide agglutination test all belonged to serotype iii. the 20 y. enterocolitica isolates were categorised bioch ...19912043083
sir frederick hobday memorial lecture. all wind and water: some progress in the study of equine gut motility. 19912044514
fumonisin mycotoxins and equine leukoencephalomalacia. 19912045319
intestinal carriage of yersinia pseudotuberculosis by wild birds and mammals in japan.fecal specimens were obtained from wild birds and mammals in the eastern part of shimane prefecture, japan, an area where serotype 1b, 2b, 3, and 4b strains of yersinia pseudotuberculosis were found to be prevalent in humans. each of 869 animals, including 259 wild birds and 610 wild mammals, was screened for yersiniae. a total of 37 strains of y. pseudotuberculosis were isolated from 34 (5.6%) mammals, including 23 raccoon dogs (nyctereutes procyonoides), eight deer (cervus nippon), two hares ( ...19912059038
antibodies against dengue viral proteins in primary and secondary dengue hemorrhagic fever.antibodies against dengue viral proteins were demonstrated in sera from dengue-infected patients by polyacrylamide gel electrophoresis, western blotting, and enzyme immunoassay. primary dengue cases showed low titers of igg class antibodies to envelope (e) proteins and two non-structural proteins, ns3 and ns5, in sera collected during the convalescent phase. secondary dengue- infected patients always demonstrated igg antibodies to e proteins in sera collected during the acute phase, and high tit ...19912063952
[the possible mechanisms of the adaptation of microbial populations to low positive temperatures].biologically low temperature was studied for its effect on the survival of populations and their biochemical and molecular-genetical state. the research was carried out on yersinia pseudotuberculosis and shigella sonnei models. it is established that the process of low-temperature adaptation of the populations may occur either at the level of the whole population with participation of inducible cold isoenzymes or at the level of a part of population, the temperature being a selection factor of t ...19912067418
[recurrent thromboembolism disclosing protein c deficiency. apropos of a case with familial investigation].every thromboembolic manifestation, especially in young subjects, calls for an aetiological study in which haemostasis is evaluated primarily with assays of physiological coagulation inhibitors: protein c, protein s and antithrombin iii. protein c deficiency is found in 6 to 7% of thromboembolic manifestations. we report the case of a 21-year old man who had phlebitis followed by pulmonary embolism without facilitating factors. protein c level was 50% of normal value (0.50 iu/ml). the patient re ...19902075365
[the entry portals and routes of yersinia pseudotuberculosis penetration into the warm-blooded organism].this work presents data indicating that y. pseudotuberculosis population, cultivated at lower temperature (6-8 degrees c), has a high potential of cellular and tissue invasiveness. pseudotuberculosis has been experimentally shown to start as generalized infection due to the rapid (during 10-15 minutes) penetration of the infective agent through the epithelium of the mouth cavity, the small intestine, the urinary bladder, conjunctiva, pulmonary alveoli and vascular walls into the blood and then i ...19902075758
treatment of yersinia infection with tetracyclines. 19902076082
[microbiology and epidemiology of yersinia infections].eleven species are actually recognized within the genus yersinia of which three--y. pestis, y. pseudotuberculosis, and certain serovars of y. enterocolitica--are important infectious organisms for humans and warm-blooded animals. the causative agents of yersiniosis, y. pseudotuberculosis and y. enterocolitica, occur world-wide in areas of moderate and subtropical climate. asymptomatically infected warm-blooded animals, causing environmental contamination, are the most important factors for the e ...19902076900
[enteropathogenic yersinias: pathogenicity factors and new diagnostic methods].a limited number of serotypes of yersinia enterocolitica and y. pseudotuberculosis is enteropathogenic for humans. during the last decade molecular biology contributed significantly to the understanding of the pathogenicity of these enteric agents. all human pathogenic yersiniae harbour a 70-kilobase plasmid which is essential for virulence expression. presently, thirteen plasmid-encoded polypeptides have been identified. two of them have been identified as true outer membrane proteins and at le ...19902076901
[infectious erythema of yersiniosis etiology].a study is presented of 14 patients (9 adults, 5 children) with infectious erythema interpreted as one of the clinical variants of generalized skin form of yersiniosis. along with typical signs of erythema, signs were found characteristic of yersiniosis, namely, mesadenitis, acute hepatitis, desquamative glossitis, changes in the ileocecal region, dyspeptic phenomena, tendency to a wave-like course. the clinical signs and symptoms of infectious erythema of yersiniosis etiology are illustrated by ...19902080559
[cloning of yersinia pseudotuberculosis calcium-dependence plasmid pyv6953 bamhi fragments and their analysis in escherichia coli mini-cells].calcium dependence plasmid pyv6953 (70.4 kb) in yersinia pseudotuberculosis cells codes for the major quantities synthesis of 150; 48.5; 19.4 kd outer membrane proteins and the 51, 38, 27 kd proteins secreted into the culturing medium. these outer membrane and secreted proteins are synthesized in considerable amounts in yersinia pseudotuberculosis strains 6953 and 9547 at 37 degrees c and in the absence of calcium ions in the culturing medium. bamhi fragments of the plasmid pyv6953 as components ...19902084542
[value of microbiologic studies for diagnosis of post-enteritis reactive arthritis].reactive arthritis may develop within a period of some days until upto 3 weeks after infections with yersinia enterocolitica, yersinia pseudotuberculosis, campylobacter jejuni/coli, shigella and salmonella. intestinal infections with klebsiella pneumoniae, clostridium perfringens, clostridium difficile, cryptosporidium, strongyloides stercoralis, taenia saginata and schistosoma mansoni are, in some cases, considered to be responsible for reactive arthritis. detection of pathogenic bacteria in fe ...19902085058
enterocolitis in pigs associated with yersinia pseudotuberculosis infection. 19902085301
[features of a protein component of the endotoxin from yersinia pseudotuberculosis].the protein moiety of endotoxin from yersinia pseudotuberculosis was found to consist of two polypeptides with apparent molecular masses 40 and 14.5 kda (4:1 w/w). the major protein (40 kda) was isolated from the endotoxin pretreated with sodium deoxy cholate by gel chromatography on the sephadex g-200 column. comparative study of this protein and oligomeric form of porin from the outer membrane of y. pseudotuberculosis using sds--page, velocity sedimentation, lipid bilayer experiments, chemical ...19902085323
effect of polyelectrolytes on entry of escherichia coli hb101 (pri203) into hela cells.the role of charged molecules in the entry mechanism of enteroinvasive bacteria was studied using escherichia coli hb101 harboring a plasmid (pri203) containing the yersinia pseudotuberculosis invasion region as an experimental model. we investigated the effect of several anionic and cationic polyelectrolytes on the initial steps of infection of hela s3 cells by e. coli hb101 (pri203). experiments in which the polyions were added to cell monolayers together with bacteria showed that invasion was ...19902087154
yersinia pseudotuberculosis infection of buffaloes (bubalus bubalis). 19902090274
enteritis in sheep, goats and pigs due to yersinia pseudotuberculosis infection.the features of naturally occurring yersinia pseudotuberculosis serotype iii infections in 16 sheep, one goat and 3 pigs, and y. pseudotuberculosis serotype i infections in 3 goats, are described. affected animals usually had diarrhoea and were in poor condition or emaciated. a number were moribund or dead when submitted for necropsy. thickening of the caecal and colonic mucosa was the only gross lesion attributable to y. pseudotuberculosis infection, with liver or other visceral abscesses not b ...19902098020
hybrid vaccines using escherichia coli as an antigen carrier. 19902101482
[an analysis of the mechanisms of interpopulation interaction of yersinia with tetrahymena pyriformis infusorians at the cellular and subcellular levels].the mechanisms of interaction between the populations of yersinia and t. pyriformis have been analyzed on the cellular and subcellular levels. as shown in this investigation, yersinia, when phagocytized by t. pyriformis, may undergo morphological changes, remain unchanged and also multiply, destroying the host cell in the process.19902109915
medium-chain-triglyceride lipid emulsion: metabolism and tissue distribution.the utilization and distribution of radioactively labeled lipid emulsions were evaluated in sprague-dawley rats. animals received one of three lipid emulsions. group 1 received [14c]medium-chain-triglyceride (mct) lipid emulsion, group 2 received a 75%:25% (vol:vol) admixture of [14c]mct: unlabeled long-chain-triglyceride (lct) lipid emulsion, and group 3 received only [14c]lct. the radioactive dose appearing in expired carbon dioxide and various body tissues was monitored over a 24-h period. re ...19902118303
differential susceptibility to dl-alpha-difluoromethylornithine in clinical isolates of trypanosoma brucei rhodesiense.dl-alpha-difluoromethylornithine is an enzyme-activated inhibitor of ornithine decarboxylase and an antagonist of polyamine metabolism that has been successful in clinical trials against west african sleeping sickness caused by trypanosoma brucei gambiense. its potential for use against the more virulent east african form of the disease, caused by t. brucei rhodesiense, is not certain. we examined 14 east african clinical isolates from the kenya trypanosomiasis research institute strain bank plu ...19902118325
phorbol myristate acetate inhibits hela 229 invasion by bordetella pertussis and other invasive bacterial pathogens.the microfilament inhibitors cytochalasins b and d have been traditionally used to indirectly evaluate the requirement for actin in the uptake of invasive bacterial pathogens by nonprofessional phagocytes. through their effects on microfilaments, both cytochalasins also impart profound alterations in cellular morphology and surface topology, which likely interfere with adherence. alterations affecting adherence would complicate interpretation of the effect of cytochalasins on entry alone. as an ...19902119340
analysis of factors which determine the existence of yersinia pseudotuberculosis in a saprophytic phase.data are presented on the effects of a variety of abiotic and biotic environmental factors on the existence and changes in the numbers of y. pseudotuberculosis. experiments with sterile soil showed that y. pseudotuberculosis populations were resistant over a wide range of major abiotic factors: temperature (0-30 degrees c), humidity (15-50%), ph (5.9-9.0). although exerting some effect on the duration of different growth phases, the above abiotic factors did not influence, within the tested rang ...19902125615
ultrastructural changes in some pathogenic bacteria after treatment with ethylendiamintetraacetic acid.the ultrastructure of pseudomonas pseudomallei, pseudomonas aeruginosa. yersinia pseudotuberculosis, salmonella typhimurium, listeria monocytogenes was studied after treatment with edta. the study established that the most sensitive species were: pseudomonas pseudomallei and pseudomonas aeruginosa. in the case of listeria monocytogenes changes were not observed. typical ultrastructural changes in the cell wall and the cytoplasmic membrane were established by means of ultrathin sections, as well ...19902129076
[the sensitivity of yersinia to bacteriophage mu].various representatives of the genus yersinia were found to differ in their sensitivity to the lytic action of bacteriophage mu cts62, which could serve as an auxiliary test for the differentiation of y. pestis and y. pseudotuberculosis. among the strains under study, the causative agents of plague (34 strains) were sensitive to phage mu cts62, while the causative agents of enteric yersiniosis (42 strains) and pseudotuberculosis (73 strains), except 3 strains with the properties of y. pestis, we ...19902146845
delay of puberty and impairment of growth in female rats given a non competitive antagonist of nmda receptors.reportedly, excitatory amino acids are involved in the control of gonadotropin secretion of rats and non-human primates. the aim of this study was to investigate the effect of chronic blockade of nmda (n-methyl-d-aspartic acid) receptors by the non competitive receptor antagonist mk-801 on gonadotropin secretion and the onset of puberty in female rats. moreover, since in humans alterations of the timing of puberty frequently coexist with disturbances of body growth, suggesting a common etiology ...19902147050
simultaneous primary infection with hiv and cmv leading to severe pancytopenia, hepatitis, nephritis, perimyocarditis, myositis, and alopecia totalis.simultaneous primary infection with hiv and cmv in an 18-year-old woman led to an acute cytotoxic reaction, manifesting as pancytopenia, hepatitis, nephritis, perimyocarditis, and myositis. within 14 days parameters indicating acute cell damage reverted to normal. two weeks later transient alopecia totalis developed. initially, hiv-antigen but no hiv antibodies were present. within 3 weeks hiv-igg antibodies appeared while hiv antigen disappeared. anti-cmv-igm was clearly and anti-cmv-igg questi ...19902156107
porin as a component of yersinia pseudotuberculosis endotoxin. 19902158213
mechanistic studies of the biosynthesis of 3,6-dideoxyhexoses in yersinia pseudotuberculosis. purification and characterization of cdp-6-deoxy-delta 3,4-glucoseen reductase based on its nadh:dichlorophenolindolphenol oxidoreductase activity.cdp-6-deoxy-delta 3,4-glucoseen reductase, the key enzyme catalyzing the biosynthetic formation of cdp-ascarylose (cdp-3,6-dideoxy-l-arabino-hexose), was purified from yersinia pseudotuberculosis by monitoring its nadh:dichlorophenolindolphenol oxidoreductase activity. a protocol consisting of deae-cellulose, phenyl-sepharose, and sephadex g-100 column chromatography yielded a mixture of two proteins. the low molecular weight protein contaminant was removed by limited tryptic digestion leaving a ...19902159466
the lcre gene is part of an operon in the lcr region of yersinia enterocolitica o:3.the low-calcium response (lcr) region of the virulence plasmid of yersinia pestis, yersinia pseudotuberculosis, and yersinia enterocolitica has been associated with calcium-dependent growth of bacteria. mutations in the previously identified lcre locus within the lcr region lack the repressor control of production of the lcr specific proteins, yersinia outer membrane proteins (yops) and v and w antigens. we sequenced a 3.3-kilobase-pair bamhi-clai fragment of the lcre locus of pyvo3, the virulen ...19902160939
[structural-functional characteristics of yersinia pseudotuberculosis plasmid pvm82].the restriction map of yersinia pseudotuberculosis plasmid pvm82 was established using the "chromosome walking" method. according to transpositional mutagenesis, the plasmid pvm82 appeared to be conjugative and was able to be transmitted from y. pseudotuberculosis to the e. coli k-12 cells.19902165015
establishment of a camptothecin analogue (cpt-11)-resistant cell line of human non-small cell lung cancer: characterization and mechanism of resistance.camptothecin-11 (cpt-11) is a new derivative of camptothecin, a plant alkaloid antitumor agent, and a good candidate for clinical trials because of higher antitumor activity, less toxicity, and high aqueous solubility. cpt-11 is known to be altered into an active form, sn-38, by esterase in in vivo. cpt-11-resistant cells (pc-7/cpt) established from a human non-small cell lung cancer cell (pc-7) by stepwise, continuous treatment with cpt-11 exhibit about a 10-fold increase in resistance to the d ...19902168285
structures of euglobal-g1, -g2, and -g3 from eucalyptus grandis, three new inhibitors of epstein-barr virus activation.three new euglobals with acylphloroglucinol-monoterpene structures, named euglobal -g1 (1), -g2 (2), and -g3 (3) were isolated from the chloroform extract of the juvenile leaves of eucalyptus grandis (myrtaceae). the structures of these new compounds were determined on the basis of their spectral data. the compounds strongly inhibited the epstein-barr virus activation.19902168298
sequence analysis of conserved rega and variable orf43.1 genes in t4-like bacteriophages.bacteriophage t4 rega protein is a translational repressor of several phage mrnas. in the t4-related phages examined, rega nucleotide sequences are highly conserved and the inferred amino acid sequences are identical. the exceptional phage, rb69, did not produce a rega protein reproducibly identifiable by western blots (immunoblots) nor did it produce mrna that hybridized to t4 rega primers. nucleotide sequences of either 223 or 250 base pairs were identified immediately 3' to rega in rb18 and r ...19902168375
calcitonin receptors on circulating normal human lymphocytes.circulating human lymphocytes possess specific and functional receptors for calcitriol and pth. we sought to determine if they also possessed receptors for calcitonin (ct), the third classical calciotropic hormone. we isolated blood mononuclear cells from healthy volunteers to separate monocytes, total lymphocytes, and t-lymphocytes; the purity of the three cell populations was more than 90%, 95%, and 85%, respectively. salmon ct (sct) was labeled by the chloramine-t method (sa, 254 muci/microgr ...19902168433
determination of bhv1 specific immune reactivity in naturally infected and vaccinated animals by lymphocyte proliferation assays.the in vitro bhv1-specific lymphocyte stimulation assay was used to investigate immune reactivity of cattle after natural infection or vaccination with bhv1. proliferative responses to live virus were shown in tests with peripheral blood lymphocytes of seropositive field virus-infected animals and of vaccinated animals. nineteen out of 36 seropositive field virus-infected animals did not show in vitro responses. nine out of 12 animals showed, at least transient, responsiveness after vaccination. ...19902168602
effect of particle size and temperature on the conformation and physiological behavior of apolipoprotein e bound to model lipoprotein particles.the effect of particle size and structural order/disorder of the lipid domain on the conformation and physiological behavior of lipid-associated apolipoprotein e (apoe) was evaluated. circular dichroic (cd) spectra of apoe bound to large (lme) and small (sme) microemulsion particles, composed of dimyristoylphosphatidylcholine (dmpc) and cholesteryl oleate (co), and to dmpc disks revealed that at 4 degrees c, where all of the lipid constituents were in an ordered state, apoe bound to lme displaye ...19902168737
alteration of the aminophospholipid translocase activity during in vivo and artificial aging of human erythrocytes.human erythrocytes were separated into three density groups representing different age groups. phospholipid outside-inside translocation rates and equilibrium distribution were determined in each group with spin-labeled phosphatidylserine (ps*), phosphatidylethanolamine (pe*), and phosphatidylcholine (pc*), at 37 degrees c and 4 degrees c. at both temperatures, the initial velocity of aminolipid translocation was reduced in the more dense (older) cells. the equilibrium distribution was not signi ...19902168752
a genetic locus of enteropathogenic escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells.the ability of enteropathogenic escherichia coli (epec) to form attaching and effacing intestinal lesions is a major characteristic of epec pathogenesis. using tnphoa mutagenesis we have identified a chromosomal gene (eae, for e. coli attaching and effacing) that is necessary for this activity. a dna probe derived from this gene hybridizes to 100% of e. coli of epec serogroups that demonstrate attaching and effacing activity on tissue culture cells as well as other pathogenic e. coli that produc ...19902172966
biphasic effects of polarizing current on adenosine-sensitive generation of cyclic amp in rat cerebral cortex.cyclic amp accumulation elicited by adenosine was investigated in cortical slices of rats following the application of a weak anodal direct current (anodal polarization) to the unilateral sensorimotor cortex. anodal polarization with 3.0 microa for 30 min caused an increase in the adenosine-elicited accumulation of cyclic amp in the polarized cortex, in which case the increase in the polarized cortical region was highlighted by repeated applications of the currents. polarization with 0.3 microa ...19902173816
[a cyclic 3',5'-adenosine monophosphate-binding protein in the causative agent of plague].camp-binding protein was isolated from the plaque agent and purified to the homogeneous state. purification process included filtration of the initial preparation through the membrane able to transmit particles with mol. weight to 300,000 da, chromatography on cellulose "de-52" and biogel htp. the protein homogeneity was confirmed by electrophoresis in polyacrylamide gel and precipitation with commercial plague agglutinating serum. the protein with mol. weight of 180,000 da consisted of two iden ...19902177132
yersinia pseudotuberculosis infection as a cause of reactive arthritis as seen in a genitourinary clinic: case report. 19902179118
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