| international control of smoking and the us experience. | international data demonstrate convincingly that lung cancer death rates follow and parallel cigarette smoking prevalence rates in both men and women. effective control of lung cancer and the many other diseases associated with smoking can be achieved only by continued reductions in smoking prevalence in developed countries and the arrestment of smoking in developing countries. smoking control strategies can take the form of prevention programs, cessation programs, regulatory and legislative mea ... | 1986 | 3956298 |
| does the pattern of tissue mobilization dictate protein requirements? | | 1986 | 3957715 |
| bubonic plague in a cat. | bubonic plague was diagnosed in a cat. the diagnosis was made by isolation of bacteria from a ruptured bubo and by demonstration of an antibody titer to yersinia pestis. the cat was treated successfully with amoxicillin and streptomycin. the entire veterinary staff was treated prophylactically with tetracycline. | 1986 | 3957763 |
| nucleotide sequence of the enterotoxin b gene from staphylococcus aureus. | the complete nucleotide sequence of the enterotoxin b gene from staphylococcus aureus, as well as the 5' and 3' flanking regions, was determined. starting from an atg initiator codon, an open reading frame encoded the enterotoxin b precursor that consisted of 266 amino acids (mr, 31,400). the 5' terminal portion of the gene encodes a signal peptide 27 amino acids long. the deduced amino acid sequence matched, with a few exceptions, the published amino acid sequence of enterotoxin b. the structur ... | 1986 | 3957869 |
| retinal constraints on orientation specificity in cat visual cortex. | most retinal ganglion cells (levick and thibos, 1982) and cortical cells (leventhal, 1983; leventhal et al., 1984) subserving peripheral vision respond best to stimuli that are oriented radially, i.e., like the spokes of a wheel with the area centralis at the hub. we have extended this work by comparing directly the distributions of orientations represented in topographically corresponding regions of retina and visual cortex. both central and peripheral regions were studied. the relations betwee ... | 1986 | 3958796 |
| [the systematic evaluation of medical procedures. apropos of the effectiveness of parallel medicine]. | | 1986 | 3961360 |
| expression of antigens encoded by the virulence plasmid of yersinia enterocolitica under different growth conditions. | the expression of polypeptides of the virulence plasmid of yersinia enterocolitica serotype o:3 was studied with the immunoblotting technique and specifically absorbed antisera to y. enterocolitica o:3. at least 16 polypeptides were apparently specified by the virulence plasmid when plasmid-bearing bacterial were grown at 37 degrees c or intraperitoneally in semipermeable capsules. the different growth media used (also with added ca2+) had quantitatively or qualitatively only a little influence ... | 1985 | 3965395 |
| survival and growth of yersinia pestis within macrophages and an effect of the loss of the 47-megadalton plasmid on growth in macrophages. | the survival and growth of yersinia pestis cells within mouse peritoneal cavities and within mouse peritoneal macrophages maintained in vitro was examined. two strains were used which differed only in that one (kim) contained the 47-megadalton plasmid associated with virulence and the second (kim1) lacked this plasmid. the kim cells, but not the kim1 cells, acquired some resistance to phagocytosis during growth at 37 degrees c which was not evident when cells were grown at 26 degrees c. whether ... | 1985 | 3965398 |
| the effect of heat shock on the cell cycle regulation of tubulin expression in physarum polycephalum. | in the myxomycete physarum polycephalum, tubulin synthesis is subject to mitotic cycle control. virtually all tubulin synthesis is limited to a 2-h period immediately preceding mitosis, and the peak of tubulin protein synthesis is accompanied by a parallel increase in the level of tubulin mrna. the mechanism by which the accumulation of tubulin mrna is turned on and off is not clear. to probe the relationship between tubulin regulation and cell cycle controls, we have used heat shocks to delay m ... | 1985 | 3968184 |
| reversible and irreversible interactions between elastin and plasma lipoproteins. | the interactions between radiolabeled, human plasma lipoproteins and elastin derived from bovine ligamentum nuchae were investigated using a washout technique. the interaction was characterised by ki, a coefficient of irreversible binding, and kr, the reversible partition coefficient. for both low-density lipoproteins (ldl) and high-density lipoproteins (hdl) the ki values decreased as total lipoprotein concentration increased, suggesting that the binding is saturable, and were similar in magnit ... | 1985 | 3970975 |
| lesioning of the rat basal forebrain leads to memory impairments in passive and active avoidance tasks. | effects of the bilateral electrolytic lesioning of the basal forebrain (bf), including the ventral globus pallidus, on passive or active avoidance tasks, were studied in male wistar rats. a severe deficit in acquisition of passive avoidance response was produced by the lesioning in the posterior level of bf. the retention of the passive avoidance response was markedly disrupted with post-training lesioning. time-dependent but only slight recovery from the memory impairments was observed in the p ... | 1985 | 3971182 |
| some problems associated with adenylate cyclase bioassays for parathyroid hormone. | a renal adenylate cyclase system was assessed for its suitability in the performance of parathyroid hormone (pth) bioassays. membrane preparations from 1 week old chicks were found to be more sensitive to pth than material from humans, dogs or rats. because of the presence of non-specific inhibitors and stimulators of adenylate cyclase in human serum, each serum sample was assayed in the presence and in the absence of a specific pth inhibitor and the difference was used to calculate pth activity ... | 1985 | 3971661 |
| effects of neuronal activity on inositol phospholipid metabolism in the rat autonomic nervous system. | the effect of nerve stimulation on inositol phospholipid hydrolysis in autonomic tissue was assessed by direct measurement of [3h]inositol phosphate production in ganglia that had been preincubated with [3h]inositol. within minutes, stimulation of the preganglionic nerve increased the [3h]inositol phosphate content of the superior cervical sympathetic ganglion indicating increased hydrolysis of inositol phospholipids. this effect was blocked in a low ca2+, high mg2+ medium. it was also greatly r ... | 1985 | 3973589 |
| [current insights into an old problem: bvd (bovine virus diarrhea)]. | a number of recent papers on the epidemiology of bovine virus diarrhoea and the pathogenesis of mucosal disease resulted in new insights, but also gave rise to several questions. prior to dealing with these questions the new views are discussed, and the possible damaging effects of bvd infection are briefly reviewed. | 1985 | 3975890 |
| ultrastructural changes in the spleen of the natterjack, bufo calamita, after antigenic stimulation. | in the present study comparative aspects of the ultrastructure of the spleen were analyzed in non-immunized and t-dependent antigen-challenged natterjacks, bufo calamita. special attention is focused on the role of the non-lymphoid components in the splenic immunoreactivity. ten days after primary immunization with sheep erythrocytes, splenic lymphoid follicles increase considerably in number and size. by that time, lymphoblasts, medium and large lymphocytes abound in the periphery of the white ... | 1985 | 3978698 |
| subunits of bovine lutropin. hormonal and immunological activity after separation with reversed-phase high-performance liquid chromatography. | by means of reversed-phase high-performance liquid chromatography, we have fractionated bovine lutropin (lh) standard preparations. the highly purified niamdd-blh-4 was fractionated into two components, while the less pure nih-lh-b9 revealed three distinct peaks. the eluted material was further characterized by in vitro bioassay and by homologous radioimmunoassay for bovine lh, ovine lh-alpha and ovine lh-beta subunits. the material with the shortest retention time possessed almost no lh-activit ... | 1985 | 3980634 |
| evaluation of the micro-id system for the identification of yersinia pestis. | one hundred isolates of yersinia pestis identified by conventional means were tested by the micro-id system to assess its reliability for distinguishing y. pestis from other members of the family enterobacteriaceae. the micro-id system gave y. pestis as a choice for the identification of 89 of these cultures, although not always as the first choice. most nitrate-negative strains of y. pestis keyed out with yersinia pseudotuberculosis as first choice and y. pestis as second or fourth choice. | 1985 | 3980695 |
| metastatic nontraumatic clostridium septicum osteomyelitis. | nontraumatic clostridial infections are rare, but need to be diagnosed and treated early or high morbidity and mortality rates result. we believe that this is the first reported case of metastatic nontraumatic clostridium septicum osteomyelitis. early treatment with surgical debridement and parenteral antibiotics without hyperbaric oxygen was used. an associated occult rectal malignancy was discovered and treated. | 1985 | 3980945 |
| [chromatin activation and various problems of genetic activity regulation in eukaryotic cells]. | in this paper the results of the investigation of activated chromatin are considered in terms of modern ideas of molecular mechanisms of genetic activity regulation. particular attention is given to the results obtained in the laboratory for functional morphology of chromosomes at the institute of molecular biology in 1973-1984. | 1985 | 3982409 |
| repression and inhibition of cephalosporin synthetases in streptomyces clavuligerus by inorganic phosphate. | cephalosporin production by growing cells of streptomyces clavuligerus was reduced by 100 mm inorganic phosphate. resting cell production was repressed by prior growth in high phosphate and inhibited by phosphate. the cell-free activity of desacetoxycephalosporin c synthetase (ring expansion activity) was repressed by prior growth in high phosphate and inhibited by phosphate. isopenicillin n synthetase (cyclase) was inhibited but not repressed. penicillin epimerase was neither inhibited nor repr ... | 1985 | 3985747 |
| complement and hla. further definition of high-risk haplotypes in insulin-dependent diabetes. | the families of 41 probands with type i (insulin-dependent) diabetes mellitus (iddm) were typed for hla-a, hla-b, and hla-dr antigens in addition to the complement polymorphisms c2, c4a, c4b, and bf. all of these loci are encoded on the short arm of human chromosome 6 in a narrow region. alleles at hla-b (8, 15, 18, and 40), hla-dr (3 and 4), and bf (f1) have been associated with increased relative risk (rr) for iddm, while hla-b7 and hla-dr2 have been associated with decreased rr for iddm. this ... | 1985 | 3987976 |
| problems concerning the usefulness of adjustment of urinary cadmium for creatinine and specific gravity. | a previous study concluded that there are some doubts as to the validity of creatinine as a parameter for adjusting the values of biological indicators determined on spot samples of urine, since it is subject to marked inter- and intraindividual variations. furthermore, since there was only a moderate correlation between creatinine levels and specific density, it can be assumed that these two parameters cannot be used indifferently for adjustment. nevertheless, it seemed advisable to verify whet ... | 1985 | 3988354 |
| [use of a drug with juvenile hormone action against flea transmitters of the plague agent]. | | 1985 | 3990641 |
| anesthetics influence myocardial infarct size. | this study compares the effect of three anesthetics on infarct size and regional myocardial blood flow. the anesthetics--fentanyl, na-pentobarbital, and halothane--differ in their effects on such hemodynamic parameters as blood pressure and heart rate. the mean blood pressure during ligation was 144/91 mm hg with fentanyl, 141/104 mm hg with na-pentobarbital, and 113/82 mm hg with halothane. the heart rate was 98, 146, and 135 beats/min, respectively. the most significant finding of our study fo ... | 1985 | 3992052 |
| [persistence of m. arthritidis and the rauscher leukemia virus in a mixed mycoplasma-viral infection in mice]. | | 1985 | 3993191 |
| the role of carbohydrate in erythropoietin action. | the carbohydrate composition of human erythropoietin (epo) was determined by micro-glc. enzymic removal of most of the sugars results in aggregation of glycosidase-treated epo, loss of biological activity when assayed in mice, and retention of activity when assayed in marrow cell cultures or by ria. endoglycosidase f causes the removal of most of the carbohydrates indicating that the oligosaccharides are asparagine linked. the lack of o-linked sugar is confirmed by the absence of n-acetylgalacto ... | 1985 | 3996313 |
| immune response to plasmid- and chromosome-encoded yersinia antigens. | the immune response of humans and mice to temperature-specific, plasmid- or chromosome-encoded proteins of yersinia pestis and yersinia enterocolitica was investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blotting. extracts from y. pestis and y. enterocolitica strains with and without the virulence plasmids pyv019 and pyv8081, respectively, were resolved by denaturing electrophoresis, and the major antigens were visualized with sera from convalescing plague pa ... | 1985 | 3997240 |
| complement activation and clearance in acute illness and injury: evidence for c5a as a cell-directed mediator of the adult respiratory distress syndrome in man. | the appearance of the adult respiratory distress syndrome (ards) during the course of acute illness is believed to result, in part, from intrapulmonary neutrophil sequestration and degranulation induced by circulating inflammatory mediators. to evaluate the role of complement-neutrophil interactions in the pathogenesis of ards in man, 34 patients suffering from intra-abdominal sepsis (seven), multisystem trauma (15), or acute pancreatitis (12) were serially studied with regard to neutrophil migr ... | 1985 | 4002115 |
| [changes in the cathepsin activity of reticuloendothelial cells under the effect of biologically active substances]. | the level of cathepsin d, an enzyme of the lysosomal origin was used as a criterion of the increase in the digestive capacity of macrophages under the action of biologically active substances. it was shown that the use of prodigiosan changed the enzyme activity in the cells of the peritoneal exudate and spleen of the albino mice. increased activity of cathepsin d was considered as a state of increased readiness for phagocytosis. | 1985 | 4004183 |
| 31p-saturation-transfer nuclear-magnetic-resonance measurements of phosphocreatine turnover in guinea-pig brain slices. | the technique of 31p saturation-transfer n.m.r. was used to determine the forward and the reverse rate constants of creatine phosphotransferase in superfused guinea-pig cerebral tissues in vitro. the calculated forward rate constant of 0.22 +/- 0.03s-1 compared well with a previously reported value for rat brain in vivo [shoubridge, briggs & radda (1982) febs lett. 140, 288-292]. the reverse rate constant was found to be 0.55 +/- 0.10s-1. 3. by using concentrations of atp and phosphocreatine est ... | 1985 | 4004799 |
| role of the anterior hypothalamus-preoptic area in the regulation of courtship behavior in the male canadian red-sided garter snake (thamnophis sirtalis parietalis): intracranial implantation experiments. | testosterone (t) was administered intracranially to intact adult male canadian red-sided garter snakes (thamnophis sirtalis parietalis) in the fall and in the summer. implants in the anterior hypothalamus-preoptic area (ah-poa) as well as other regions of the brain, including the medial and basal hypothalamus, thalamus, medial cortex, and third ventricle, failed to elicit courtship behavior. the spermatogenic stage was more advanced, and the circulating level of androgens was significantly highe ... | 1985 | 4007796 |
| [studies of mechanisms of diarrhea induced by fusarenon-x, a trichothecene mycotoxin from fusarium species; the effects of fusarenon-x and various cathartics on the digestion and the absorption of the mouse intestine]. | | 1985 | 4009421 |
| effect of severe injury and critical illness on high-energy phosphates in human liver and muscle. | changes in hepatic and muscle high-energy phosphates in varying degrees of resting hypermetabolism were studied. fourteen severely injured and five critically ill patients with normal blood pressure were investigated: the results were compared with 14 normal controls. atp and adp levels in the muscle were significantly changed in acutely severe injury: lactate and pyruvate levels in the liver and muscle increased; glycogen level in the liver decreased. meanwhile, high-energy phosphates in both l ... | 1985 | 4009768 |
| middle cerebral artery reconstruction. | the authors have conducted an experimental study of the microanatomic features of the middle cerebral artery in unfixed human brains to assess the feasibility of reconstructive procedures within the sylvian fissure. it was found that reconstructive procedures within the sylvian fissure such as end-to-end, end-to-side, side-to-side, and grafting procedures were possible according to the anatomic data provided by our study. possible surgical solutions to theoretical pathologic situations involving ... | 1985 | 4012560 |
| is salmonellosis still a serious problem in veterinary practice? | salmonella infections of food animals are of concern to both the food industry and to the public health authorities. salmonellosis is one of the major zoonotic diseases and its impact on veterinary practice may be judged by a number of criteria. some of these are examined to determine the extent of the problem. | 1985 | 4013036 |
| an indication for human tetanus immunoglobulin in unimmunized patients. | | 1985 | 4015799 |
| antimicrotubule effects of estramustine, an antiprostatic tumor drug. | estramustine [17 beta-estradiol 3 n bis(2-chloroethyl)carbamate; em] is a stable conjugate of estradiol and nor-nitrogen mustard that is used for the treatment of human prostatic carcinoma. we have studied the cytotoxic effects of em on the cytoskeletal organization of squirrelfish pigment cells (erythrophores) and human prostatic tumor cells (du 145) in culture. light and whole-mount electron microscopy studies reveal that, at microm levels (60 to 120 microm), em has a dose-dependent disruptive ... | 1985 | 4016756 |
| a profile of the intestinal mucosal corticosteroid receptors in the domestic duck. | the corticosteroid receptor profile of the intestinal tract of the domestic duck (maintained on either a low-sodium (ls) or a high-sodium (hs) diet) was investigated. using tritiated triamcinolone acetonide (ta), corticosterone, or aldosterone as ligands, cytoplasmic mineralocorticoid receptors (mr, type i) and glucocorticoid receptors (gr, type ii) were found in the mucosal cytosol of the jejunum and colon with the following binding parameters: ls jejunum gr-kd, 3.4 nm; nmax, 245 fmol/mg protei ... | 1985 | 4018554 |
| ultrastructural studies of adherence of staphylococcus aureus in experimental acute hematogenous osteomyelitis. | a model of acute hematogenous osteomyelitis initiated by injection of staphylococcus aureus into 29-day-old chickens was used. transmission electron microscopy showed that the bacteria adhered to exposed cartilage matrix in the metaphyseal region of long bones but not to adjacent vascular linings or to erythrocytes. it is proposed that the combination of exposure of growth plate cartilage during normal bone growth and the ability of s. aureus to adhere to this cartilage is the mechanism for init ... | 1985 | 4018875 |
| the temporal evolution of hypoglycemic brain damage. i. light- and electron-microscopic findings in the rat cerebral cortex. | in the course of a study on the pathogenesis of neuronal necrosis in severe hypoglycemia, the morphological characteristics reflecting reversible and irreversible neuronal lesions were examined as a function of time following normalization of blood glucose. to that end, closely spaced time intervals were studied in the rat cerebral cortex before, during, and up to 1 year after standardized pure hypoglycemic insults of 30 and 60 min of cerebral isoelectricity. both the superficial and deep layers ... | 1985 | 4024866 |
| factors modifying cytotoxicity induced by 5-fu and hydroxyurea. | pretreatment of exponentially growing 9l cells for 24 hours with a nontoxic (0.77 microm) or moderately toxic (7.7 microm) concentration of 5-fu (5-fura) enhanced cell kill induced by a subsequent 6-hour treatment with 1.3 mm hydroxyurea (hu) by a magnitude of 1 log. in confluent 9l cultures or 9l cells treated in serum-free medium that decreased the cycling population, however, the 5-fura-hu combination did not enhance the cell kill. no recovery from potentially lethal damage was observed for u ... | 1985 | 4028040 |
| factors modifying cytotoxicity induced by 5-fu and hydroxyurea. | pretreatment of exponentially growing 9l cells for 24 hours with a nontoxic (0.77 microm) or moderately toxic (7.7 microm) concentration of 5-fu (5-fura) enhanced cell kill induced by a subsequent 6-hour treatment with 1.3 mm hydroxyurea (hu) by a magnitude of 1 log. in confluent 9l cultures or 9l cells treated in serum-free medium that decreased the cycling population, however, the 5-fura-hu combination did not enhance the cell kill. no recovery from potentially lethal damage was observed for u ... | 1985 | 4028040 |
| lipopolysaccharides from yersinia pestis. studies on lipid a of lipopolysaccharides i and ii. | the chemical structure of the lipid a of lipopolysaccharide i and ii from yersinia pestis, strain ev 40, was studied. it consists of a (1 ---- 6), beta-linked d-glucosamine disaccharide which carries two phosphate groups; one phosphate is linked glycosidically with a glucosamine unit, the other one is linked to the non-reducing glucosamine. various degradation methods combined with 31p nuclear magnetic resonance spectroscopy showed that the ester-bound phosphate group is linked to a 4-aminoarabi ... | 1985 | 4029140 |
| species differences in the handling of lysosomotropic metals and triton wr 1339 by rat and chinese hamster liver. | the study was undertaken in order to understand the reasons for the distinct differences in the elimination rate of lanthanides and transuranium elements from the liver of different mammalian species. the binding of monomeric 239pu in livers of rats and chinese hamsters was analyzed by density gradient centrifugation and electrophoresis. it was concluded that this nuclide is initially bound to lysosomes in liver of rats and chinese hamsters. the influence of triton wr 1339 (twr) on the density o ... | 1985 | 4029172 |
| size and density characterization of human committed and multipotent hematopoietic progenitors. | physical characterization of human blood and bone marrow (bm) hematopoietic precursors is necessary for the design of adequate techniques to isolate these cells for experimental and possible clinical use. elutriation and continuous percoll gradient were used to define the size and density of committed (cfu-gm, bfu-e, and cfu-e) and multipotent (cfu-mix) progenitors on paired bm and blood samples from normal donors. cells were initially prepared over ficoll-hypaque (specific gravity 1.077 g/cm3). ... | 1985 | 4029293 |
| arterial thrombosis as the cause of lameness in a foal. | a 3-day-old foal was examined because of forelimb lameness. brachial artery occlusion was diagnosed. the foal was euthanatized. necropsy revealed brachial artery thrombosis and an atrial septal defect. | 1985 | 4030453 |
| assessment of protein quality methodology for infant formulas. | rat bioassay was used to assess the protein quality of powdered infant formulas and to evaluate the feasibility of using modified casein diets (containing the same source and level of fat and carbohydrate contributed by the infant formulas) as reference standards. modification of the casein diet to match the milk-based formulas caused a significant reduction in weekly protein efficiency ratios (per) and net protein ratios (npr) at the third and fourth weeks. modification of the casein diet to st ... | 1985 | 4030638 |
| experimental plague in rock squirrels, spermophilus variegatus (erxleben). | experimental infections with yersinia pestis were followed in groups of rock squirrels. development of coagulopathy and pneumonia were observed in 2-4% and 11-12% of the test animals, respectively. susceptibility to experimental infection was heterogeneous with some animals surviving inoculation with large numbers of organisms and others succumbing after inoculation with small numbers. production and longevity of serum antibody titers, as measured by passive hemagglutination tests, were variable ... | 1985 | 4032620 |
| schistosoma mansoni: an ultrastructural examination of skin migration in the hamster cheek pouch. | the hamster cheek pouch has been used to investigate the skin migration of schistosomula at the ultrastructural level. parasites have been observed up to 72 h post-infection. despite rapidly reaching the epidermal basement membrane schistosomula remain in an epidermal location for at least 40 h post-infection, by which time the acetabular glands and their ducts have been lost and the transformation from cercaria to schistosomulum completed. entry into the dermis and exit from the skin via the bl ... | 1985 | 4034241 |
| the interaction of representative members from two classes of antimycotics--the azoles and the allylamines--with cytochromes p-450 in steroidogenic tissues and liver. | spectrophotometric studies with ketoconazole, clotrimazole and miconazole show strong type-ii interactions with several cytochromes p-450, particularly (ks greater than 10(7)m-1; ph7.4; 25 degrees c) with the 11 beta-hydroxylase of adrenal mitochondria, with the 17 alpha/20 lyase of testis microsomes and with some forms of cytochromes p-450 of liver. a tight binding of the azoles also occurs to the reduced cytochromes, giving rise to an impeded co binding to the haem iron. the binding of the azo ... | 1985 | 4036174 |
| local cerebral glucose utilization in normal female rats: variations during the estrous cycle and comparison with males. | the quantitative 2-[14c]deoxyglucose autoradiographic method was used to study the fluctuations of energy metabolism in discrete brain regions of female rats during the estrous cycle. a consistent though statistically nonsignificant cyclic variation in average glucose utilization of the brain as a whole was observed. highest levels of glucose utilization occurred during proestrus and metestrus, whereas lower rates were found during estrus and diestrus. statistically significant fluctuations were ... | 1985 | 4040921 |
| anti-major histocompatibility complex immunity detected prior to intentional alloimmunization. ii. monoclonal h-2-specific antibodies obtained from an unimmunized c57bl/kalwrij (h-2b) mouse. | a monoclonal 'natural' anti-h-2 igm antibody produced by a hybridoma cell line ol-3.17 (h-2 m. 209) is described. the ol-3.17 monoclonal antibody was obtained by hybridization of spleen b cells from an unimmunized c57bl/ka (h-2b) mouse in the serum of which simultaneously an igm kappa paraprotein of high concentration and a natural h-2-specific antibody of high titer was detected. the monoclonal antibody ol-3.17 reacted strongly with h-2d and h-2s and weakly with h-2k,q,r lymphocytes, thereby de ... | 1985 | 4047072 |
| [characteristics of the multiplication of a virulent strain of the plague microbe in xenopsylla cheopis fleas infected parenterally]. | xenopsylla cheopis fleas infected parenterally with the virulent strain of plague microbe of gerbil variant preserved the agent to the end of their lives. in the body cavity the microbes retained their ability for reproduction which was, however, limited. during the first seven days after the infection the number of microbes slightly increased and later became stabilized. its mean indices (mean g) varied within the limits of 500 to 2000 microbe cells per 1 individual, maximum index rarely exceed ... | 1985 | 4047718 |
| trypanosoma brucei: the extent of conversion in antigen genes may be related to the dna coding specificity. | the boundaries of gene conversion in variant-specific antigen genes have been determined in six clones of trypanosoma brucei. in each clone, antigenic switching involved interaction between two telomeric members of the antat 1.1 multigene family, which share extensive homology throughout their coding regions. all conversion events occurred by substitution of faithful copies of donor sequences. conversion endpoints were nonrandomly distributed. in four clones, the 5' conversion limit was near the ... | 1985 | 4053185 |
| day to day variations in morphology and duration of fragmented ventricular potentials during the late post-myocardial infarction phase in conscious dogs. | the relationship between the inducibility of ventricular tachyarrhythmias and the presence of fractionated epicardial ventricular electrograms ('late potentials') was studied daily between days 3 and 8 after experimental myocardial infarction in 15 conscious dogs. before each programmed stimulation, epicardial infarct zone electrograms were recorded from implanted 'composite' electrodes during sinus rhythm. there was considerable daily variation in the morphology and duration of delayed ventricu ... | 1985 | 4054137 |
| regulation of low density lipoprotein receptor synthesis in cultured luteinized human granulosa cells by human chorionic gonadotropin and 8-bromo-cyclic amp. | we investigated the regulation of synthesis of low density lipoprotein (ldl) receptor in cultured luteinized human granulosa cells using a monoclonal antibody recognizing the human ldl receptor (igg-c7). cells cultured under serum-free conditions were treated with human chorionic gonadotropin (hcg) or 8-bromo-camp alone or in combination with aminoglutethimide (to block conversion of cholesterol to steroid hormones) and 5-cholesten-3 beta, 25-diol (25-hydroxycholesterol, a potent suppressor of l ... | 1985 | 4055779 |
| effect of subchronic cholestasis on microsomal mixed-function oxidases and the glutathione-conjugating enzyme system in rat liver. | bile duct ligation in male rats for two weeks led to a marked increase in both serum sorbitol dehydrogenase activity and serum bile acid concentration indicating cholestatic liver injury. furthermore, a rise in the hepatic hydroxyproline level indicating collagen accumulation was observed. as a consequence of these alterations, the hepatic microsomal mixed-function oxidase system was impaired as evidenced by a decrease in cytochrome p-450 content and in the activities of nadph-cytochrome c reduc ... | 1985 | 4059319 |
| [properties of strains of the causative agent of plague resistant to the diagnostic phage l-413 "c"]. | the data obtained in the comparative study of the lytic activity of two plague diagnostic phages, pokrovskaya's phage and phage l-413 "c", with respect to 655 y. pestis strains are presented. the study revealed the advantage of pokrovskaya's phage which lyzed all the strains under test over phage l-413 "c", found to be inactive with respect to 27 y. pestis strains. the properties of the strains resistant to phage l-413 "c" were typical for the causative agent of plague in the corresponding natur ... | 1985 | 4060951 |
| hypoxic pulmonary vasoconstriction is not potentiated by repeated intermittent hypoxia in closed chest dogs. | hypoxic pulmonary vasoconstrictor (hpv) responses were measured with repeated intermittent hypoxic challenges in eight non-traumatized closed chest dogs anesthetized with pentobarbital. the right lung was ventilated continuously with 100% o2 while the left lung was either ventilated with 100% o2 (control) or ventilated with a gas mixture containing 3-4% o2 (hypoxia). mean per cent left lung blood flow for all four normoxic periods was 43.1 +/- 1.5% (mean +/- se) of the total blood flow by the sf ... | 1985 | 4061916 |
| arterial subsegments of the human kidney. | | 1985 | 4062476 |
| [inhibition of cardiac activity by the stellate ganglion]. | mechanisms of cardiac activity inhibition in response to the excitation of the stellate ganglion were investigated in acute and chronic experiments involving guinea pigs (43) and rabbits (35). the inhibitory effect was shown to proceed from the excitation of vagal cholinergic fibres that anastomosed with branches of the stellate ganglion, while alpha-adrenoreceptors had no apparent effect on cardiac activity. neither the hypothesis of preganglionic sympathetic fibres switching to intracardiac ch ... | 1985 | 4068467 |
| fibronectin and f-actin redistribution in cultured endothelial cells exposed to shear stress. | cultured endothelial cells exposed to shear stresses in vitro undergo a reorganization of their f-actin-containing cytoskeletons which culminates in realignment with flow direction. since a close transmembrane association exists between actin microfilaments and extracellular fibronectin, this study was undertaken to examine whether the actin reorganization induced by shear stress is accompanied by perturbations in the underlying fibronectin matrix. in a closed circulatory loop, bovine endothelia ... | 1985 | 4068668 |
| importance of frequency and pulse with in field stimulation of the mouse vas deferens: different behaviour of twitch-inhibiting drugs. | the effect of six twitch-inhibiting drugs on the stimulus-response relationship in the field-stimulated mouse vas deferens was compared by means of stimulus response curves which were obtained in two ways, that is, variation of frequency at constant pulse width and variation of pulse width at constant frequency. the twitch-inhibiting potency (in the range of maximal twitch responses) differed with the type of stimulation in a way permitting two groups of substances to be defined: group a (tetrod ... | 1985 | 4069233 |
| importance of frequency and pulse with in field stimulation of the mouse vas deferens: different behaviour of twitch-inhibiting drugs. | the effect of six twitch-inhibiting drugs on the stimulus-response relationship in the field-stimulated mouse vas deferens was compared by means of stimulus response curves which were obtained in two ways, that is, variation of frequency at constant pulse width and variation of pulse width at constant frequency. the twitch-inhibiting potency (in the range of maximal twitch responses) differed with the type of stimulation in a way permitting two groups of substances to be defined: group a (tetrod ... | 1985 | 4069233 |
| radiofrequency dielectric properties of animal tissues as a function of time following death. | the dielectric properties of three bovine tissues, liver, kidney and spleen, as a function of time following death, were measured in the frequency range from 20 khz to 100 mhz using an automatic network analyser and an end-of-the-line sensor. the dielectric constant of kidney and spleen decreases as a function of time following death, particularly at frequencies below 1 mhz. however, all tissues measured show a characteristic increase in the frequency-independent ionic conductivity. this is beli ... | 1985 | 4070369 |
| [study of the aggregation of membrane proteins of erythrocyte ghosts using sds-paag electrophoresis]. | using the method of electrophoresis in sds-paag the authors showed a diminution of proteins of bands i + ii (spectrins) and iii (major integral protein) after irradiation of erythrocyte ghosts with doses of 50 to 1000 gy. we failed to ascertain that radiation-induced lipid peroxidation is involved into membrane protein aggregation. among the radiolysis products, oh-radicals were shown to contribute markedly to the radiation effect observed. | 1985 | 4070566 |
| immunopathology and histopathology of experimental chlamydial salpingitis. | experimental infection of fallopian tubes was produced in four sexually mature pig-tailed monkeys (macaca nemestrina) by intratubal inoculation with serovar e or f chlamydia trachomatis. infection was confirmed by reisolation of the organism from both the endosalpinx and the endocervix. an antibody response to the infecting strain of c. trachomatis was demonstrated in monkey sera and in cervical secretions by the microimmunofluorescence test. a cellular immune response to c. trachomatis was show ... | 1985 | 4070908 |
| interdependence of regional expiratory flow. | flows from different lung regions interact at the junctions of the bronchial tree, and flow from each region depends on the driving pressures for other regions. at each junction, flow from the region with the higher driving pressure is favored. as a result there is a limit on the difference in alveolar pressures that can develop during expiratory flow from a lung with regional differences in lung compliance and airway resistance. the limiting pressure difference is smaller for lower flow. a nonu ... | 1985 | 4077799 |
| [immunological study of anti-cancer effects of human alpha interferon for advanced renal cell carcinoma]. | | 1985 | 4079199 |
| [local anesthetic effects of morphine and naloxone]. | the effects of both morphine (mo) and naloxone (nal), topically applied to the desheathed saphenous nerve, were studied in sprague-dawley rats during 22 min of superfusion at various concentrations (mo: 0.086, 0.28, 0.86, 2.8, 8.6 mm/l; nal: 0.1, 0.33, 1.0 mm/l) by recording single c-fibres. c-fibres were electrically stimulated and the latencies of action potentials were measured. both drugs impaired nerve conduction in a dose-dependent, fully reversible manner. at high concentrations, conducti ... | 1985 | 4091249 |
| muscle cross-bridge kinetics in rigor and in the presence of atp analogues. | recently we reported preliminary mechanical experiments on freshly skinned rabbit psoas fibers that suggested that while almost all of the cross-bridges are attached to actin in the presence of 4 mm adenyl-5'-yl-imidodiphosphate (amp-pnp) (ionic strength, 0.13 m), there is an equilibrium between the attached and detached states, so that, in the presence of 4 mm amp-pnp, fibers should not be able to maintain tension (schoenberg, et al., 1984, in contractile mechanisms in muscle, pollack and sugi, ... | 1985 | 4092069 |
| evidence for the exchangeability of acidic ribosomal proteins on cytoplasmic ribosomes in regenerating rat liver. | we purified acidic ribosomal proteins (p1 and p2) in good yield from rat liver ribosomes by precipitation of ribosomes with mgcl2 prior to ethanol extraction and chromatography of the extract on a column of cm-cellulose at ph 4.8. the newly-synthesized acidic ribosomal proteins in regenerating rat liver, labeled in vivo with [3h]leucine, were rapidly incorporated into cytoplasmic ribosomes without any detectable time lag and, after reaching a maximum at 30 min, they gradually disappeared from th ... | 1985 | 4093437 |
| working with human cell lines. | | 1970 | 4097614 |
| prenatal and early postnatal ontogenesis of the human motor cortex: a golgi study. ii. the basket-pyramidal system. | | 1970 | 4097698 |
| studies on equine immunoglobulins. i. the antigenic structure of horse igg, its fragments and subunits. | | 1970 | 4097705 |
| [development of polyribosomes during maturation of reticulocytes in vivo]. | | 1970 | 4098024 |
| escherichia coli serotypes and renal involvement in urinary-tract infection. | | 1971 | 4103393 |
| [immunochemistry of pasteurella pestis. i. determinants common to p. pestis ev 76 and enterobacteiaceae]. | | 1971 | 4105123 |
| effect of oral contraceptive steroids on jejunal pyruvate kinase and adenyl cyclase activities. | | 1971 | 4105307 |
| [immunochemistry of pasteurella pestis. ii. heterogeneity of antigen synthesis]. | | 1971 | 4108683 |
| perfusion of isolated human fetal liver: synthesis and release of -fetoprotein and albumin. | | 1971 | 4109106 |
| vestibulo-ocular responses in man during sleep. | | 1972 | 4109914 |
| histamine release by inorganic cations from mast cell granules isolated by different procedures. | | 1972 | 4113827 |
| the value of 2,4-bis(n,n'-di(carboxymethyl) aminomethyl) fluorescein as a bone marking dye. | | 1972 | 4117251 |
| [mechanism of accumulation and discharge of a capsular antigen by pasteurella pestis (role of the cell wall)]. | | 1972 | 4118969 |
| morphologica and structural changes during the yeast-to mold conversion of phialophora dermatitidis. | the details of the morphological and structural events occurring during yeast-to-mold conversion of the human pathogenic fungus phialophora dermatitidis as seen by phase-contrast microscopy and electron microscopy are described and illustrated. budding yeasts growing exponentially were observed to have thin walls and a cytoplasm exhibiting the characteristics of rapidly growing cells including numerous mitochondria, abundant ribosomes, few vacuoles, and little accumulation of storage material. i ... | 1973 | 4120203 |
| detection of the "pregnancy zone" protein by means of an immunodiffusion method. | | 1973 | 4120261 |
| detection of the "pregnancy zone" protein by means of an immunodiffusion method. | | 1973 | 4120261 |
| cathepsin d activity in normal and osteoarthritic human cartilage. | | 1973 | 4121833 |
| cellular reactions against burkitt lymphoma cells. ii. effector cells obtained by allogeneic stimulation in mixed leukocyte cultures. | | 1972 | 4122453 |
| [absolute control of the transcription of the adenovirus 2 genome in kb cells]. | | 1973 | 4122931 |
| [a micromethod for fractionation and identification of bacterial proteins]. | | 1972 | 4124512 |
| antigen modulation of the immune response. the decline of immunological memory in the absence of continuing antigenic stimulation. | | 1973 | 4125287 |
| cerebrospinal-fluid igm in patients with sleeping-sickness. | | 1973 | 4125294 |
| [alkaline phosphatase isoenzymes in human blood serum]. | | 1973 | 4125468 |
| characterization of bleomycin-resistant dna. | after reaction of dna with high concentrations of bleomycin, approximately 80% of the dna becomes trichloroacetic acid (tca) soluble. the remaining 20% of the dna remains tca insoluble. upon further treatment of this tca-insoluble material with high concentrations of the drug, no further drug action can be detected. drug action is defined as fragmentation of dna to smaller molecular size, release of free bases, and tca solubilization. this material which is not attacked by bleomycin has been ter ... | 1973 | 4128368 |
| [diagnostic value of serological reactions for detecting antibodies against the plague microbe]. | | 1973 | 4129113 |
| plague in vietnam. clinical observations and treatment with kanamycin. | | 1974 | 4130297 |
| [regulation of synthesis of the capsular antigen of pasteurella pestis by components of the nutrient medium]. | | 1973 | 4131408 |
| introductory immunologic studies on human gastrointestinal tract tumors. | | 1973 | 4131722 |