Publications

[antigenic activity of external membrane proteins in yersinia pestis ev].	the study of immunocomplexes obtained from antisera to the preparations of y. pestis outer membranes and membrane proteins has revealed that outer membrane proteins are involved in the formation of the immunocomplex and belong to previously unknown y. pestis ev antigens.	1983	6405562
[detection and characterization of the plasmids of the plague microbe which determine the synthesis of pesticin i, fraction i antigen and "mouse" toxin exotoxin].	plasmid dna was isolated from yersinia pestis strains containing pesticin i or fraction i antigen and "mouse" toxin determinants. specificity of dna preparations was studied by using them for transformation of plague agent strains carrying no plasmids. ppsti plasmid (molecular weight 7,0-7,8 md) encoded pesticin i, fibrinolysin and plasmacoagulase synthesis. fraction i antigen and "mouse" toxin production determinants were borne on pfrai/tox plasmid (molecular weight about 50 md). the observatio ...	1983	6194044
[methods of preparing and purifying the capsular antigen and endotoxin of the causative agent of plague].		1983	6201660
[quantitative characteristics of the survival phenomenon in experimental plague].	the method for the quantitative evaluation of the effect of the survival phenomenon is proposed, which makes it possible to carry out the rapid single-stage determination of the immunogenicity of pasteurella pestis avirulent strains. the low specificity of the survival phenomenon in plague has been shown. the accumulation of immunogenic avirulent cells in the population of virulent p. pestis has been found to decrease their pathogenic action to a greater extent than the accumulation of nonimmuno ...	1983	6362297
use of uv-irradiated bacteriophage t6 to kill extracellular bacteria in tissue culture infectivity assays.	we have utilized 'lysis from without' mediated by uv-inactivated bacteriophage t6 to eliminate extracellular bacteria in experiments measuring the internalization, intracellular survival and replication of yersinia pestis within mouse peritoneal macrophages and of shigella flexneri within a human intestinal epithelial cell line. the technique we describe has the following characteristics: (a) bacterial killing is complete within 15 min at 37 degrees c, with a greater than 10(3)-fold reduction in ...	1983	6338113
constitutive uptake and degradation of fatty acids by yersinia pestis.	yersinia pestis was found to utilize palmitic acid as a primary carbon and energy source. no inhibition of growth by palmitic acid was observed. comparison of palmitic acid uptake by cells pregrown either with or without palmitic acid demonstrated that fatty acid uptake was constitutive. high basal levels of two enzymes of beta-oxidation, beta-hydroxyacyl-coenzyme a dehydrogenase and thiolase, and the two enzymes of the glyoxylate shunt, isocitrate lyase and malate synthase, were found in cells ...	1983	6129238
[concentration and purification of d'erell's phage].		1983	6086049
[construction of a suspension diagnosticum to test for yersinia pestis endotoxin and homologous antibodies].		1983	6195409
the role of the association in education for the profession.		1983	264831
photoinduced crosslinking of membrane proteins by fluorescein isothiocyanate.		1983	217376
left ventricular stiffness.	left ventricular stiffness is defined by the relationship between pressure and volume and can be altered by a variety of disorders. in disorders with high vlved, such as mitral regurgitation, protection of pulmonary capillaries from high pressure is provided by a pv shift. in contrast, increased stiffness is produced by ventricular hypertrophy or ischemic heart disease, which puts the patient at increased risk for pulmonary capillary hypertension. changing the ventricular pv relationship by ther ...	1983	348043
leukocyte function after aortic valve replacement.	leukocyte function was studied in patients with prosthetic heart valves by oxygen consumption measurements during phagocytosis of polystyrene latex particles. the consumption reflects the phagocytotic capacity of the cells. in 38 patients with starr-edwards aortic ball valves the mean oxygen consumption was 3.95 nanoatoms per minute per 10(6) leukocytes, as compared to 4.15 in 50 healthy subjects, the difference not being statistically significant. the number of leukocytes per ml. of blood and t ...	1984	263395
a simple method of eminoplasty for correction of recurrent dislocation of the mandible.	a simple surgical method of eminoplasty has been described that is rapid and has few complications. its value in the treatment of recurrent dislocation of the mandible is now well established. whether the procedure works by providing greater freedom of motion or by limitation is a moot point as the operation is highly successful.	1984	264267
[detection of a specific plague microbe antigen (fraction i) by the method of radioimmunological analysis].		1984	6200692
genetic analysis of the low calcium response in yersinia pestis mu d1(ap lac) insertion mutants.	yersinia pestis strain kim requires plasmid pcd1 for expression of the low calcium response, plague virulence antigen v, and virulence. we constructed mu d1(ap lac) insertion mutants of this plasmid which were unable to express the low calcium response. the insertions mapped to a 17-kilobase region of the plasmid. by determining the orientation of the insertions and examining beta-galactosidase production from the mu d1 lac genes, we determined that this region contains three units of transcript ...	1984	6094509
[comparison of specific recognition sites of adenine and cytosine dna-methylase of yersinia pestis ev 76 c dam and dcm by escherichia coli methylases].	using enzymatic modelling of in vitro methylation of chromosome dnas from yersinia pestis ev 76, e. coli 834 and e. coli c600 rii by dna methylases of eco rii and eco dam as well as of dna hydrolysis of plasmid pbr 322 from the cells of y. pestis ev 76, e. coli c600 and e. coli 834 by restrictases of eco rii and cfu i, it was found that cytosine dna methylase from plague bacteria does not correspond to the type of rii methylases of e. coli. adenyl dna methylase is related to e. coli methylases t ...	1984	6097301
[a possible way of realizing a signal of increased temperature for plague microbe cultivation].		1984	6101075
cross reactions in pha test for plague antibodies.		1984	6535790
role of the virulence-associated plasmids of yersinia enterocolitica on its immunogenicity against y. pestis.	forty-two strains selected from various serovars of yersinia enterocolitica were studied for their ability to induce cross-protection against y. pestis. only primo-infected mice (i.v.) with inocula prepared from strains of y. enterocolitica belonging to serovars 0:3, 0:9 and 0:5, 27 harbouring a 47-mdal virulence-associated plasmid were resistant to y. pestis. the spontaneously cured (plasmid-less) derivatives of these strains had lost the capacity to induce this cross-immunity.	1984	6532281
[use of monoclonal antibodies in immunoenzyme analysis for demonstrating antigenic monovalency].	to prove the monovalence of the antigen a method has been developed consisting of elisa with the use of monoclonal antibodies in combination with the antibody neutralization test. yersinia pestis capsular antigen was disintegrated by heating at 100 degrees c for a short time and subsequently passed through a column packed with sephadex g-50. the portions of the eluate, showing high activity in the antibody neutralization test and low activity in elisa (the double antibody sandwich scheme), conta ...	1984	6395594
[choice of the optimal technic for immunofluorescence staining using microfluorometry].		1984	6210412
[isolation, purification and physicochemical properties of pesticin i from cells of yersinia pestis strain ev].	pesticin i has been isolated and purified from y. pestis strain ev. the homogeneous preparation of pesticin has been shown to be monomer protein with a molecular weight of 65000 daltons, having three immunologically identical alpha-, beta- and gamma-forms with different isoelectric points. the amino acid composition of pesticin i is presented. rabbit anti-serum to the beta-form of the preparation of pesticin has been obtained.	1984	6431718
growth in mouse peritoneal macrophages of yersinia pestis lacking established virulence determinants.	cultured mouse resident peritoneal macrophages were challenged with strains of yersinia pestis differing only with respect to the absence of one or more of the virulence determinants established for the species. guanine-auxotrophic (pur-) yersiniae were unable to survive within the macrophages; exogenous hypoxanthine and guanosine permitted intracellular growth. this finding supports the idea that pur- yersiniae are avirulent due to inability to obtain sufficient free purines in host tissues for ...	1984	6469351
yersinia pestis grows within phagolysosomes in mouse peritoneal macrophages.	transmission electron microscopy was used to determine the intracellular localization of yersinia pestis growing within cultured resident peritoneal macrophages of mice. monolayers fixed immediately, or as long as 4.5 h, after infection contained yersiniae closely surrounded by a membrane. the vesicles containing the yersiniae were determined to be phagolysosomes by labeling secondary lysosomes of macrophages with electron-dense particles of thorium dioxide. the presence of the label within vesi ...	1984	6469352
effect of chlorination on antibiotic resistance profiles of sewage-related bacteria.	a total of 1,900 lactose-fermenting bacteria were isolated from raw sewage influent and chlorinated sewage effluent from a sewage treatment plant, as well as from chlorinated and neutralized dilute sewage, before and after a 24-h regrowth period in the laboratory. of these isolates, 84% were resistant to one or more antibiotics. chlorination of influent resulted in an increase in the proportion of bacteria resistant to ampicillin and cephalothin, the increase being most marked after regrowth occ ...	1984	6476832
[method of disinfecting plague-infected fleas in preparation for electron microscopic study].	during fixation of fleas infected with plague agent (with preliminary cut heads, limbs and posterior part of the abdomen) in 2.5% glutaric dialdehyde or 2% osmium tetroxide their disinfection was obtained in two days. after double fixation with 2-hour exposure in each of these fixators disinfection of the material was acheived only after additional 2-day maintenance in 70% ethyl alcohol. fleas, which had been placed into fixators or in 70% ethyl alcohol after double fixation without preliminary ...	1984	6483471
a comparison of serological techniques for plague surveillance.	sera collected from 691 dogs and 231 rodents in south africa were tested in parallel by the passive haemagglutination (pha) and enzyme-linked immunosorbent assay (elisa) for antibodies to the fraction 1 antigen of the plague bacillus (yersinia pseudotuberculosis subsp. pestis). there was a high degree of correlation between the tests, both in over-all numbers positive and in serum titres. 42 dog sera were positive by pha and 41 by elisa, 40 sera being positive by both tests. however, using elisa ...	1984	6533848
characterization of common virulence plasmids in yersinia species and their role in the expression of outer membrane proteins.	the virulence plasmids pyv019, pyv8081, and pib1 from yersinia pestis, yersinia enterocolitica, and yersinia pseudotuberculosis, respectively, were characterized by restriction endonuclease analysis. the three plasmids exhibited a region of common dna previously shown to encode determinants which confer ca2+ dependence. the plasmids from y. pestis and y. pseudotuberculosis were similar throughout their genomes. in contrast, a region of the plasmid from y. enterocolitica which contained an origin ...	1984	6317562
evidence for the extinction of plague in hawaii.	plague (infection by yersinia pestis) was present in hawaii for the period 1899-1957 and caused at least 370 fatalities. the first infections came from immigrant commensal rats, probably rattus rattus and rattus norvegicus, on ships from the orient. both species were already established in hawaii and became the widespread local carriers of plague, supplemented by rattus exulans which had colonized the islands in ancient polynesian times. the flea xenopsylla vexabilis arrived with r. exulans, and ...	1984	6364800
in vivo comparison of avirulent vwa- and pgm- or pstr phenotypes of yersiniae.	the abilities of yersinia pestis to undergo restriction in ca2+-deficient medium with concomitant production of v and w antigens (vwa+) and to absorb exogenous pigments (pgm+) are established virulence factors. mutation of y. pestis to pgm- is known to promote resistance to pesticin (pstr) and reduced lethality by peripheral routes of injection. vwa+ pgm- isolates of y. pestis were shown in this study to retain virulence in mice when injected intravenously. although pgm- in appearance, wild-type ...	1984	6365786
human bubonic plague transmitted by a domestic cat scratch.	bubonic plague was transmitted to a 10-year-old girl in oregon by a scratch wound inflicted by a domestic cat. the cat probably was infected by contact with infected wild rodents or their fleas. yersinia pestis was identified in diamanus montanus fleas collected from an abandoned burrow near the patient's home. domestic cats may infect humans with y pestis by inoculation from a scratch.	1984	6694293
[results of experiments simulating the preservation of the causative agent of plague under natural conditions].		1984	6727783
[effectiveness of revaccinating hamadryas baboons with niss live dried plague vaccine and fraction i of the plague microbe].	the effectiveness of some vaccine preparations for the revaccination of hamadryas baboons after their primary immunization with live plague vaccine " niis " administered in the form of aerosol was studied. the study was carried out under the conditions of the aerosol challenge of the animals with y. pestis. the subcutaneous injection of plague vaccine " niis " was found to have advantages over its aerosol administration. revaccination with y. pestis fraction i, absorbed, was found to be 8 times ...	1984	6741359
potential for rat plague from nonencapsulated variants of the plague bacillus (yersinia pestis).	potentials for oral and flea-borne transmission of nonencapsulated y. pestis were demonstrated when 45% of rats that consumed infected meat died of plague and 22% of the rats that died of plague had bacteremia.	1984	6745406
roles of v antigen in promoting virulence and immunity in yersiniae.	it is established that yersiniae harboring an approximately 45-megadalton vwa-plasmid can produce v and w antigens (vwa+), and that sera containing anti-v provides passive protection to mice against yersinia pestis. this observation was extended by the use of monospecific anti-v prepared by injecting rabbits with partially purified v, absorption of antisera with a vwa- extract, and then separation of gamma-globulin by traditional processes of fractionation or by affinity chromatography. these pr ...	1984	6381597
primary plague pneumonia contracted from a domestic cat at south lake tahoe, calif.	primary plague pneumonia occurred in a 47-year-old south lake tahoe woman shortly after face-to-face exposure to her plague pneumonia-infected cat. both died. field investigation revealed a recent plague epizootic in squirrels and chipmunks around the patient's home. control measures included active surveillance and chemoprophylaxis of 197 contacts to the victim, a community alert on methods of self- and pet protection, and application of insecticide to reduce rodent flea populations. no seconda ...	1984	6694294
plague and the peripheral smear.		1984	6694298
a projection from the deep layers of the entorhinal area to the hippocampal formation in the rat brain.	the methods of retrograde fluorescent tracing and anterograde transport of the lectin phaseolus vulgaris leucoagglutinin (pha-l) were used to demonstrate the existence of projections from layers iv and vi of the entorhinal area to the hippocampal formation in the rat brain. these two layers of the medial and lateral entorhinal area innervate the molecular layer of ammon's horn and the area dentata. in the area dentata the projection from layer iv follows that of the perforant path, while that fr ...	1985	2409482
a study on the pharmacokinetics in mouse of adenine-9-beta-d-arabinofuranoside 5-monophosphate conjugated with lactosaminated albumin.	in plasma of mice injected with adenine-9-beta-d-arabinofuranoside monophosphate (ara-amp) coupled to human lactosaminated serum albumin (l-hsa) some of the ara-amp molecules are enzymatically released, whereas others remain linked to l-hsa. evidence has been obtained that ara-amp is not deaminated when it is conjugated to l-hsa, in contrast to the free drug which is rapidly metabolized to its hypoxanthine derivative.	1985	2412878
monoclonal antibodies reactive with epitopes restricted to glial fibrillary acidic proteins of several species.	the highly reproducible histochemical localization of glial fibrillary acidic protein (gfap)+ qualifies it as an important marker of astrocytes in both research and clinical applications. the primary objective of this study was to produce monoclonal antibodies having the advantage of invariant specificity, affinity, and titer to gfap-specific epitopes of wide species distribution. we report here the characterization of four monoclonal antibodies that recognize the same or spatially close epitope ...	1985	2413405
[vital staining of the conjunctiva for prevention of epithelial implantation cysts].	selective vital staining with 1% toluidine blue makes short-term intraoperative differentiation of the conjunctiva from tenon's capsule possible. it is used with advantage in operations where the anatomical situation is unclear to avoid displacement of conjunctival epithelium into deeper tissues and subsequent growth of cysts. examples of its application are reoperations with excessive scars after squint or detachment surgery, wound dehiscence after enucleations, lacerated wounds after accidents ...	1985	2415747
[requirements and standardization of the conditions for carrying out the microfluorometric test].		1985	2415765
[use of hemin as a stimulator of the growth of the plague microbe].		1985	2582200
[local anesthetic effects of morphine and naloxone].	the effects of both morphine (mo) and naloxone (nal), topically applied to the desheathed saphenous nerve, were studied in sprague-dawley rats during 22 min of superfusion at various concentrations (mo: 0.086, 0.28, 0.86, 2.8, 8.6 mm/l; nal: 0.1, 0.33, 1.0 mm/l) by recording single c-fibres. c-fibres were electrically stimulated and the latencies of action potentials were measured. both drugs impaired nerve conduction in a dose-dependent, fully reversible manner. at high concentrations, conducti ...	1985	4091249
effect of subchronic cholestasis on microsomal mixed-function oxidases and the glutathione-conjugating enzyme system in rat liver.	bile duct ligation in male rats for two weeks led to a marked increase in both serum sorbitol dehydrogenase activity and serum bile acid concentration indicating cholestatic liver injury. furthermore, a rise in the hepatic hydroxyproline level indicating collagen accumulation was observed. as a consequence of these alterations, the hepatic microsomal mixed-function oxidase system was impaired as evidenced by a decrease in cytochrome p-450 content and in the activities of nadph-cytochrome c reduc ...	1985	4059319
muscle cross-bridge kinetics in rigor and in the presence of atp analogues.	recently we reported preliminary mechanical experiments on freshly skinned rabbit psoas fibers that suggested that while almost all of the cross-bridges are attached to actin in the presence of 4 mm adenyl-5'-yl-imidodiphosphate (amp-pnp) (ionic strength, 0.13 m), there is an equilibrium between the attached and detached states, so that, in the presence of 4 mm amp-pnp, fibers should not be able to maintain tension (schoenberg, et al., 1984, in contractile mechanisms in muscle, pollack and sugi, ...	1985	4092069
evidence for the exchangeability of acidic ribosomal proteins on cytoplasmic ribosomes in regenerating rat liver.	we purified acidic ribosomal proteins (p1 and p2) in good yield from rat liver ribosomes by precipitation of ribosomes with mgcl2 prior to ethanol extraction and chromatography of the extract on a column of cm-cellulose at ph 4.8. the newly-synthesized acidic ribosomal proteins in regenerating rat liver, labeled in vivo with [3h]leucine, were rapidly incorporated into cytoplasmic ribosomes without any detectable time lag and, after reaching a maximum at 30 min, they gradually disappeared from th ...	1985	4093437
local cerebral glucose utilization in normal female rats: variations during the estrous cycle and comparison with males.	the quantitative 2-[14c]deoxyglucose autoradiographic method was used to study the fluctuations of energy metabolism in discrete brain regions of female rats during the estrous cycle. a consistent though statistically nonsignificant cyclic variation in average glucose utilization of the brain as a whole was observed. highest levels of glucose utilization occurred during proestrus and metestrus, whereas lower rates were found during estrus and diestrus. statistically significant fluctuations were ...	1985	4040921
radiofrequency dielectric properties of animal tissues as a function of time following death.	the dielectric properties of three bovine tissues, liver, kidney and spleen, as a function of time following death, were measured in the frequency range from 20 khz to 100 mhz using an automatic network analyser and an end-of-the-line sensor. the dielectric constant of kidney and spleen decreases as a function of time following death, particularly at frequencies below 1 mhz. however, all tissues measured show a characteristic increase in the frequency-independent ionic conductivity. this is beli ...	1985	4070369
anti-major histocompatibility complex immunity detected prior to intentional alloimmunization. ii. monoclonal h-2-specific antibodies obtained from an unimmunized c57bl/kalwrij (h-2b) mouse.	a monoclonal 'natural' anti-h-2 igm antibody produced by a hybridoma cell line ol-3.17 (h-2 m. 209) is described. the ol-3.17 monoclonal antibody was obtained by hybridization of spleen b cells from an unimmunized c57bl/ka (h-2b) mouse in the serum of which simultaneously an igm kappa paraprotein of high concentration and a natural h-2-specific antibody of high titer was detected. the monoclonal antibody ol-3.17 reacted strongly with h-2d and h-2s and weakly with h-2k,q,r lymphocytes, thereby de ...	1985	4047072
size and density characterization of human committed and multipotent hematopoietic progenitors.	physical characterization of human blood and bone marrow (bm) hematopoietic precursors is necessary for the design of adequate techniques to isolate these cells for experimental and possible clinical use. elutriation and continuous percoll gradient were used to define the size and density of committed (cfu-gm, bfu-e, and cfu-e) and multipotent (cfu-mix) progenitors on paired bm and blood samples from normal donors. cells were initially prepared over ficoll-hypaque (specific gravity 1.077 g/cm3). ...	1985	4029293
importance of frequency and pulse with in field stimulation of the mouse vas deferens: different behaviour of twitch-inhibiting drugs.	the effect of six twitch-inhibiting drugs on the stimulus-response relationship in the field-stimulated mouse vas deferens was compared by means of stimulus response curves which were obtained in two ways, that is, variation of frequency at constant pulse width and variation of pulse width at constant frequency. the twitch-inhibiting potency (in the range of maximal twitch responses) differed with the type of stimulation in a way permitting two groups of substances to be defined: group a (tetrod ...	1985	4069233
the interaction of representative members from two classes of antimycotics--the azoles and the allylamines--with cytochromes p-450 in steroidogenic tissues and liver.	spectrophotometric studies with ketoconazole, clotrimazole and miconazole show strong type-ii interactions with several cytochromes p-450, particularly (ks greater than 10(7)m-1; ph7.4; 25 degrees c) with the 11 beta-hydroxylase of adrenal mitochondria, with the 17 alpha/20 lyase of testis microsomes and with some forms of cytochromes p-450 of liver. a tight binding of the azoles also occurs to the reduced cytochromes, giving rise to an impeded co binding to the haem iron. the binding of the azo ...	1985	4036174
importance of frequency and pulse with in field stimulation of the mouse vas deferens: different behaviour of twitch-inhibiting drugs.	the effect of six twitch-inhibiting drugs on the stimulus-response relationship in the field-stimulated mouse vas deferens was compared by means of stimulus response curves which were obtained in two ways, that is, variation of frequency at constant pulse width and variation of pulse width at constant frequency. the twitch-inhibiting potency (in the range of maximal twitch responses) differed with the type of stimulation in a way permitting two groups of substances to be defined: group a (tetrod ...	1985	4069233
[properties of strains of the causative agent of plague resistant to the diagnostic phage l-413 "c"].	the data obtained in the comparative study of the lytic activity of two plague diagnostic phages, pokrovskaya's phage and phage l-413 "c", with respect to 655 y. pestis strains are presented. the study revealed the advantage of pokrovskaya's phage which lyzed all the strains under test over phage l-413 "c", found to be inactive with respect to 27 y. pestis strains. the properties of the strains resistant to phage l-413 "c" were typical for the causative agent of plague in the corresponding natur ...	1985	4060951
hypoxic pulmonary vasoconstriction is not potentiated by repeated intermittent hypoxia in closed chest dogs.	hypoxic pulmonary vasoconstrictor (hpv) responses were measured with repeated intermittent hypoxic challenges in eight non-traumatized closed chest dogs anesthetized with pentobarbital. the right lung was ventilated continuously with 100% o2 while the left lung was either ventilated with 100% o2 (control) or ventilated with a gas mixture containing 3-4% o2 (hypoxia). mean per cent left lung blood flow for all four normoxic periods was 43.1 +/- 1.5% (mean +/- se) of the total blood flow by the sf ...	1985	4061916
arterial subsegments of the human kidney.		1985	4062476
[immunological study of anti-cancer effects of human alpha interferon for advanced renal cell carcinoma].		1985	4079199
[inhibition of cardiac activity by the stellate ganglion].	mechanisms of cardiac activity inhibition in response to the excitation of the stellate ganglion were investigated in acute and chronic experiments involving guinea pigs (43) and rabbits (35). the inhibitory effect was shown to proceed from the excitation of vagal cholinergic fibres that anastomosed with branches of the stellate ganglion, while alpha-adrenoreceptors had no apparent effect on cardiac activity. neither the hypothesis of preganglionic sympathetic fibres switching to intracardiac ch ...	1985	4068467
fibronectin and f-actin redistribution in cultured endothelial cells exposed to shear stress.	cultured endothelial cells exposed to shear stresses in vitro undergo a reorganization of their f-actin-containing cytoskeletons which culminates in realignment with flow direction. since a close transmembrane association exists between actin microfilaments and extracellular fibronectin, this study was undertaken to examine whether the actin reorganization induced by shear stress is accompanied by perturbations in the underlying fibronectin matrix. in a closed circulatory loop, bovine endothelia ...	1985	4068668
lipopolysaccharides from yersinia pestis. studies on lipid a of lipopolysaccharides i and ii.	the chemical structure of the lipid a of lipopolysaccharide i and ii from yersinia pestis, strain ev 40, was studied. it consists of a (1 ---- 6), beta-linked d-glucosamine disaccharide which carries two phosphate groups; one phosphate is linked glycosidically with a glucosamine unit, the other one is linked to the non-reducing glucosamine. various degradation methods combined with 31p nuclear magnetic resonance spectroscopy showed that the ester-bound phosphate group is linked to a 4-aminoarabi ...	1985	4029140
experimental plague in rock squirrels, spermophilus variegatus (erxleben).	experimental infections with yersinia pestis were followed in groups of rock squirrels. development of coagulopathy and pneumonia were observed in 2-4% and 11-12% of the test animals, respectively. susceptibility to experimental infection was heterogeneous with some animals surviving inoculation with large numbers of organisms and others succumbing after inoculation with small numbers. production and longevity of serum antibody titers, as measured by passive hemagglutination tests, were variable ...	1985	4032620
interdependence of regional expiratory flow.	flows from different lung regions interact at the junctions of the bronchial tree, and flow from each region depends on the driving pressures for other regions. at each junction, flow from the region with the higher driving pressure is favored. as a result there is a limit on the difference in alveolar pressures that can develop during expiratory flow from a lung with regional differences in lung compliance and airway resistance. the limiting pressure difference is smaller for lower flow. a nonu ...	1985	4077799
factors modifying cytotoxicity induced by 5-fu and hydroxyurea.	pretreatment of exponentially growing 9l cells for 24 hours with a nontoxic (0.77 microm) or moderately toxic (7.7 microm) concentration of 5-fu (5-fura) enhanced cell kill induced by a subsequent 6-hour treatment with 1.3 mm hydroxyurea (hu) by a magnitude of 1 log. in confluent 9l cultures or 9l cells treated in serum-free medium that decreased the cycling population, however, the 5-fura-hu combination did not enhance the cell kill. no recovery from potentially lethal damage was observed for u ...	1985	4028040
[study of the aggregation of membrane proteins of erythrocyte ghosts using sds-paag electrophoresis].	using the method of electrophoresis in sds-paag the authors showed a diminution of proteins of bands i + ii (spectrins) and iii (major integral protein) after irradiation of erythrocyte ghosts with doses of 50 to 1000 gy. we failed to ascertain that radiation-induced lipid peroxidation is involved into membrane protein aggregation. among the radiolysis products, oh-radicals were shown to contribute markedly to the radiation effect observed.	1985	4070566
regulation of low density lipoprotein receptor synthesis in cultured luteinized human granulosa cells by human chorionic gonadotropin and 8-bromo-cyclic amp.	we investigated the regulation of synthesis of low density lipoprotein (ldl) receptor in cultured luteinized human granulosa cells using a monoclonal antibody recognizing the human ldl receptor (igg-c7). cells cultured under serum-free conditions were treated with human chorionic gonadotropin (hcg) or 8-bromo-camp alone or in combination with aminoglutethimide (to block conversion of cholesterol to steroid hormones) and 5-cholesten-3 beta, 25-diol (25-hydroxycholesterol, a potent suppressor of l ...	1985	4055779
immunopathology and histopathology of experimental chlamydial salpingitis.	experimental infection of fallopian tubes was produced in four sexually mature pig-tailed monkeys (macaca nemestrina) by intratubal inoculation with serovar e or f chlamydia trachomatis. infection was confirmed by reisolation of the organism from both the endosalpinx and the endocervix. an antibody response to the infecting strain of c. trachomatis was demonstrated in monkey sera and in cervical secretions by the microimmunofluorescence test. a cellular immune response to c. trachomatis was show ...	1985	4070908
is salmonellosis still a serious problem in veterinary practice?	salmonella infections of food animals are of concern to both the food industry and to the public health authorities. salmonellosis is one of the major zoonotic diseases and its impact on veterinary practice may be judged by a number of criteria. some of these are examined to determine the extent of the problem.	1985	4013036
subunits of bovine lutropin. hormonal and immunological activity after separation with reversed-phase high-performance liquid chromatography.	by means of reversed-phase high-performance liquid chromatography, we have fractionated bovine lutropin (lh) standard preparations. the highly purified niamdd-blh-4 was fractionated into two components, while the less pure nih-lh-b9 revealed three distinct peaks. the eluted material was further characterized by in vitro bioassay and by homologous radioimmunoassay for bovine lh, ovine lh-alpha and ovine lh-beta subunits. the material with the shortest retention time possessed almost no lh-activit ...	1985	3980634
some problems associated with adenylate cyclase bioassays for parathyroid hormone.	a renal adenylate cyclase system was assessed for its suitability in the performance of parathyroid hormone (pth) bioassays. membrane preparations from 1 week old chicks were found to be more sensitive to pth than material from humans, dogs or rats. because of the presence of non-specific inhibitors and stimulators of adenylate cyclase in human serum, each serum sample was assayed in the presence and in the absence of a specific pth inhibitor and the difference was used to calculate pth activity ...	1985	3971661
effects of neuronal activity on inositol phospholipid metabolism in the rat autonomic nervous system.	the effect of nerve stimulation on inositol phospholipid hydrolysis in autonomic tissue was assessed by direct measurement of [3h]inositol phosphate production in ganglia that had been preincubated with [3h]inositol. within minutes, stimulation of the preganglionic nerve increased the [3h]inositol phosphate content of the superior cervical sympathetic ganglion indicating increased hydrolysis of inositol phospholipids. this effect was blocked in a low ca2+, high mg2+ medium. it was also greatly r ...	1985	3973589
metastatic nontraumatic clostridium septicum osteomyelitis.	nontraumatic clostridial infections are rare, but need to be diagnosed and treated early or high morbidity and mortality rates result. we believe that this is the first reported case of metastatic nontraumatic clostridium septicum osteomyelitis. early treatment with surgical debridement and parenteral antibiotics without hyperbaric oxygen was used. an associated occult rectal malignancy was discovered and treated.	1985	3980945
[chromatin activation and various problems of genetic activity regulation in eukaryotic cells].	in this paper the results of the investigation of activated chromatin are considered in terms of modern ideas of molecular mechanisms of genetic activity regulation. particular attention is given to the results obtained in the laboratory for functional morphology of chromosomes at the institute of molecular biology in 1973-1984.	1985	3982409
survival and growth of yersinia pestis within macrophages and an effect of the loss of the 47-megadalton plasmid on growth in macrophages.	the survival and growth of yersinia pestis cells within mouse peritoneal cavities and within mouse peritoneal macrophages maintained in vitro was examined. two strains were used which differed only in that one (kim) contained the 47-megadalton plasmid associated with virulence and the second (kim1) lacked this plasmid. the kim cells, but not the kim1 cells, acquired some resistance to phagocytosis during growth at 37 degrees c which was not evident when cells were grown at 26 degrees c. whether ...	1985	3965398
immune response to plasmid- and chromosome-encoded yersinia antigens.	the immune response of humans and mice to temperature-specific, plasmid- or chromosome-encoded proteins of yersinia pestis and yersinia enterocolitica was investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blotting. extracts from y. pestis and y. enterocolitica strains with and without the virulence plasmids pyv019 and pyv8081, respectively, were resolved by denaturing electrophoresis, and the major antigens were visualized with sera from convalescing plague pa ...	1985	3997240
repression and inhibition of cephalosporin synthetases in streptomyces clavuligerus by inorganic phosphate.	cephalosporin production by growing cells of streptomyces clavuligerus was reduced by 100 mm inorganic phosphate. resting cell production was repressed by prior growth in high phosphate and inhibited by phosphate. the cell-free activity of desacetoxycephalosporin c synthetase (ring expansion activity) was repressed by prior growth in high phosphate and inhibited by phosphate. isopenicillin n synthetase (cyclase) was inhibited but not repressed. penicillin epimerase was neither inhibited nor repr ...	1985	3985747
complement and hla. further definition of high-risk haplotypes in insulin-dependent diabetes.	the families of 41 probands with type i (insulin-dependent) diabetes mellitus (iddm) were typed for hla-a, hla-b, and hla-dr antigens in addition to the complement polymorphisms c2, c4a, c4b, and bf. all of these loci are encoded on the short arm of human chromosome 6 in a narrow region. alleles at hla-b (8, 15, 18, and 40), hla-dr (3 and 4), and bf (f1) have been associated with increased relative risk (rr) for iddm, while hla-b7 and hla-dr2 have been associated with decreased rr for iddm. this ...	1985	3987976
problems concerning the usefulness of adjustment of urinary cadmium for creatinine and specific gravity.	a previous study concluded that there are some doubts as to the validity of creatinine as a parameter for adjusting the values of biological indicators determined on spot samples of urine, since it is subject to marked inter- and intraindividual variations. furthermore, since there was only a moderate correlation between creatinine levels and specific density, it can be assumed that these two parameters cannot be used indifferently for adjustment. nevertheless, it seemed advisable to verify whet ...	1985	3988354
anesthetics influence myocardial infarct size.	this study compares the effect of three anesthetics on infarct size and regional myocardial blood flow. the anesthetics--fentanyl, na-pentobarbital, and halothane--differ in their effects on such hemodynamic parameters as blood pressure and heart rate. the mean blood pressure during ligation was 144/91 mm hg with fentanyl, 141/104 mm hg with na-pentobarbital, and 113/82 mm hg with halothane. the heart rate was 98, 146, and 135 beats/min, respectively. the most significant finding of our study fo ...	1985	3992052
[persistence of m. arthritidis and the rauscher leukemia virus in a mixed mycoplasma-viral infection in mice].		1985	3993191
the temporal evolution of hypoglycemic brain damage. i. light- and electron-microscopic findings in the rat cerebral cortex.	in the course of a study on the pathogenesis of neuronal necrosis in severe hypoglycemia, the morphological characteristics reflecting reversible and irreversible neuronal lesions were examined as a function of time following normalization of blood glucose. to that end, closely spaced time intervals were studied in the rat cerebral cortex before, during, and up to 1 year after standardized pure hypoglycemic insults of 30 and 60 min of cerebral isoelectricity. both the superficial and deep layers ...	1985	4024866
[characteristics of the multiplication of a virulent strain of the plague microbe in xenopsylla cheopis fleas infected parenterally].	xenopsylla cheopis fleas infected parenterally with the virulent strain of plague microbe of gerbil variant preserved the agent to the end of their lives. in the body cavity the microbes retained their ability for reproduction which was, however, limited. during the first seven days after the infection the number of microbes slightly increased and later became stabilized. its mean indices (mean g) varied within the limits of 500 to 2000 microbe cells per 1 individual, maximum index rarely exceed ...	1985	4047718
effect of severe injury and critical illness on high-energy phosphates in human liver and muscle.	changes in hepatic and muscle high-energy phosphates in varying degrees of resting hypermetabolism were studied. fourteen severely injured and five critically ill patients with normal blood pressure were investigated: the results were compared with 14 normal controls. atp and adp levels in the muscle were significantly changed in acutely severe injury: lactate and pyruvate levels in the liver and muscle increased; glycogen level in the liver decreased. meanwhile, high-energy phosphates in both l ...	1985	4009768
middle cerebral artery reconstruction.	the authors have conducted an experimental study of the microanatomic features of the middle cerebral artery in unfixed human brains to assess the feasibility of reconstructive procedures within the sylvian fissure. it was found that reconstructive procedures within the sylvian fissure such as end-to-end, end-to-side, side-to-side, and grafting procedures were possible according to the anatomic data provided by our study. possible surgical solutions to theoretical pathologic situations involving ...	1985	4012560
complement activation and clearance in acute illness and injury: evidence for c5a as a cell-directed mediator of the adult respiratory distress syndrome in man.	the appearance of the adult respiratory distress syndrome (ards) during the course of acute illness is believed to result, in part, from intrapulmonary neutrophil sequestration and degranulation induced by circulating inflammatory mediators. to evaluate the role of complement-neutrophil interactions in the pathogenesis of ards in man, 34 patients suffering from intra-abdominal sepsis (seven), multisystem trauma (15), or acute pancreatitis (12) were serially studied with regard to neutrophil migr ...	1985	4002115
an indication for human tetanus immunoglobulin in unimmunized patients.		1985	4015799
antimicrotubule effects of estramustine, an antiprostatic tumor drug.	estramustine [17 beta-estradiol 3 n bis(2-chloroethyl)carbamate; em] is a stable conjugate of estradiol and nor-nitrogen mustard that is used for the treatment of human prostatic carcinoma. we have studied the cytotoxic effects of em on the cytoskeletal organization of squirrelfish pigment cells (erythrophores) and human prostatic tumor cells (du 145) in culture. light and whole-mount electron microscopy studies reveal that, at microm levels (60 to 120 microm), em has a dose-dependent disruptive ...	1985	4016756
[changes in the cathepsin activity of reticuloendothelial cells under the effect of biologically active substances].	the level of cathepsin d, an enzyme of the lysosomal origin was used as a criterion of the increase in the digestive capacity of macrophages under the action of biologically active substances. it was shown that the use of prodigiosan changed the enzyme activity in the cells of the peritoneal exudate and spleen of the albino mice. increased activity of cathepsin d was considered as a state of increased readiness for phagocytosis.	1985	4004183
31p-saturation-transfer nuclear-magnetic-resonance measurements of phosphocreatine turnover in guinea-pig brain slices.	the technique of 31p saturation-transfer n.m.r. was used to determine the forward and the reverse rate constants of creatine phosphotransferase in superfused guinea-pig cerebral tissues in vitro. the calculated forward rate constant of 0.22 +/- 0.03s-1 compared well with a previously reported value for rat brain in vivo [shoubridge, briggs & radda (1982) febs lett. 140, 288-292]. the reverse rate constant was found to be 0.55 +/- 0.10s-1. 3. by using concentrations of atp and phosphocreatine est ...	1985	4004799
role of the anterior hypothalamus-preoptic area in the regulation of courtship behavior in the male canadian red-sided garter snake (thamnophis sirtalis parietalis): intracranial implantation experiments.	testosterone (t) was administered intracranially to intact adult male canadian red-sided garter snakes (thamnophis sirtalis parietalis) in the fall and in the summer. implants in the anterior hypothalamus-preoptic area (ah-poa) as well as other regions of the brain, including the medial and basal hypothalamus, thalamus, medial cortex, and third ventricle, failed to elicit courtship behavior. the spermatogenic stage was more advanced, and the circulating level of androgens was significantly highe ...	1985	4007796
[studies of mechanisms of diarrhea induced by fusarenon-x, a trichothecene mycotoxin from fusarium species; the effects of fusarenon-x and various cathartics on the digestion and the absorption of the mouse intestine].		1985	4009421
a profile of the intestinal mucosal corticosteroid receptors in the domestic duck.	the corticosteroid receptor profile of the intestinal tract of the domestic duck (maintained on either a low-sodium (ls) or a high-sodium (hs) diet) was investigated. using tritiated triamcinolone acetonide (ta), corticosterone, or aldosterone as ligands, cytoplasmic mineralocorticoid receptors (mr, type i) and glucocorticoid receptors (gr, type ii) were found in the mucosal cytosol of the jejunum and colon with the following binding parameters: ls jejunum gr-kd, 3.4 nm; nmax, 245 fmol/mg protei ...	1985	4018554
ultrastructural studies of adherence of staphylococcus aureus in experimental acute hematogenous osteomyelitis.	a model of acute hematogenous osteomyelitis initiated by injection of staphylococcus aureus into 29-day-old chickens was used. transmission electron microscopy showed that the bacteria adhered to exposed cartilage matrix in the metaphyseal region of long bones but not to adjacent vascular linings or to erythrocytes. it is proposed that the combination of exposure of growth plate cartilage during normal bone growth and the ability of s. aureus to adhere to this cartilage is the mechanism for init ...	1985	4018875
the role of carbohydrate in erythropoietin action.	the carbohydrate composition of human erythropoietin (epo) was determined by micro-glc. enzymic removal of most of the sugars results in aggregation of glycosidase-treated epo, loss of biological activity when assayed in mice, and retention of activity when assayed in marrow cell cultures or by ria. endoglycosidase f causes the removal of most of the carbohydrates indicating that the oligosaccharides are asparagine linked. the lack of o-linked sugar is confirmed by the absence of n-acetylgalacto ...	1985	3996313
assessment of protein quality methodology for infant formulas.	rat bioassay was used to assess the protein quality of powdered infant formulas and to evaluate the feasibility of using modified casein diets (containing the same source and level of fat and carbohydrate contributed by the infant formulas) as reference standards. modification of the casein diet to match the milk-based formulas caused a significant reduction in weekly protein efficiency ratios (per) and net protein ratios (npr) at the third and fourth weeks. modification of the casein diet to st ...	1985	4030638
species differences in the handling of lysosomotropic metals and triton wr 1339 by rat and chinese hamster liver.	the study was undertaken in order to understand the reasons for the distinct differences in the elimination rate of lanthanides and transuranium elements from the liver of different mammalian species. the binding of monomeric 239pu in livers of rats and chinese hamsters was analyzed by density gradient centrifugation and electrophoresis. it was concluded that this nuclide is initially bound to lysosomes in liver of rats and chinese hamsters. the influence of triton wr 1339 (twr) on the density o ...	1985	4029172
schistosoma mansoni: an ultrastructural examination of skin migration in the hamster cheek pouch.	the hamster cheek pouch has been used to investigate the skin migration of schistosomula at the ultrastructural level. parasites have been observed up to 72 h post-infection. despite rapidly reaching the epidermal basement membrane schistosomula remain in an epidermal location for at least 40 h post-infection, by which time the acetabular glands and their ducts have been lost and the transformation from cercaria to schistosomulum completed. entry into the dermis and exit from the skin via the bl ...	1985	4034241
arterial thrombosis as the cause of lameness in a foal.	a 3-day-old foal was examined because of forelimb lameness. brachial artery occlusion was diagnosed. the foal was euthanatized. necropsy revealed brachial artery thrombosis and an atrial septal defect.	1985	4030453
trypanosoma brucei: the extent of conversion in antigen genes may be related to the dna coding specificity.	the boundaries of gene conversion in variant-specific antigen genes have been determined in six clones of trypanosoma brucei. in each clone, antigenic switching involved interaction between two telomeric members of the antat 1.1 multigene family, which share extensive homology throughout their coding regions. all conversion events occurred by substitution of faithful copies of donor sequences. conversion endpoints were nonrandomly distributed. in four clones, the 5' conversion limit was near the ...	1985	4053185
day to day variations in morphology and duration of fragmented ventricular potentials during the late post-myocardial infarction phase in conscious dogs.	the relationship between the inducibility of ventricular tachyarrhythmias and the presence of fractionated epicardial ventricular electrograms ('late potentials') was studied daily between days 3 and 8 after experimental myocardial infarction in 15 conscious dogs. before each programmed stimulation, epicardial infarct zone electrograms were recorded from implanted 'composite' electrodes during sinus rhythm. there was considerable daily variation in the morphology and duration of delayed ventricu ...	1985	4054137