| the effect of 1,25-dihydroxyvitamin d3 on the relationship between growth and differentiation in hl-60 cells. | cells of the human promyelocytic leukaemia line hl-60 may be induced to differentiate along the monocytic lineage by the seco-steroid hormone 1,25-dihydroxyvitamin d3. we used fluorescence-activated cell sorting to show that, in addition to inducing differentiation, the hormone caused hl-60 cells to accumulate in g1/g0 phases of the cell cycle. we also labelled differentiating cells with both a monocyte-specific antibody and a dna stain simultaneously. analysis of these cell populations showed t ... | 1987 | 3469486 |
| hepatitis b surface antigen gene expression is regulated by sex steroids and glucocorticoids in transgenic mice. | we have investigated the basis for liver-specific and sex-linked expression of hepatitis b surface antigen (hbsag) gene in transgenic mice by monitoring the level of liver hbsag mrna and serum hbsag at different stages of development and in response to sex-hormone regulation. transcription of the hbsag gene starts at day 15 of development, together with that of the albumin gene, and reaches a comparable level at birth. hbsag mrna level and hbsag production are parallel in males and females durin ... | 1987 | 3469661 |
| regional variations in oral submucous fibrosis in india. | regional variations in the characteristics of submucous fibrosis were studied in two districts in india. in pune district this condition involved soft palate, uvula and retromolar areas significantly more often than in ernakulam district. the tongue, floor of the mouth and the hard palate were not involved in pune. the age of the patients in pune district was lower than in ernakulam district. associated oral cancer, leukoplakia and petechiae were observed solely among patients in ernakulam. the ... | 1987 | 3476249 |
| retinal transplants can drive a pupillary reflex in host rat brains. | retinae taken from embryonic rats were transplanted over the midbrain of neonatal rats, from whom one eye had been removed. after 5 months, the optic nerve of the remaining eye was cut, and the transplant was exposed. illumination of the transplant caused pupilloconstriction of the host eye, a response abolished by damaging the transplant. thus neural transplants are capable of driving specific reflexes in response to natural stimuli. | 1987 | 3477821 |
| effects of dietary fish oil on renal insufficiency in rats with subtotal nephrectomy. | we studied the effects of fish oil on the progression of renal insufficiency in rats with subtotal nephrectomy. five weeks after a 1-2/3 nephrectomy, sixteen rats were fed two different diets which differed only in fat composition. lipid in the control diet was primarily beef tallow; that of the experimental diet, menhaden oil. fish oil-fed rats had significant increases in plasma creatinines, decreases in urinary pge2 and accelerated death rates. an additional twelve rats underwent 1-1/3 nephre ... | 1987 | 3480973 |
| gardnerella vaginitis--a guide to identification and management for the practitioner. | | 1986 | 3485708 |
| comparative antibacterial activity of an arylglycyl oral cephalosporin, ly164846. | ly164846 is a semisynthetic arylglycyl cephalosporin which can be absorbed orally. it had in vitro activity comparable to that of cefaclor against beta-hemolytic streptococcal species and was two- to fourfold more active than cephalexin. enterococci and listeria species were resistant, and its activity against staphylococci was similar to that of other oral cephalosporins. although some bacteroides species were inhibited, the mics for 25% were greater than or equal to 16 micrograms/ml. ly164846 ... | 1986 | 3486632 |
| possible role of protein phosphorylation in the mitogenic effect of high density lipoproteins on cultured vascular endothelial cells. | the implication of protein phosphorylation in the mitogenic action of high density lipoproteins (hdl) on bovine vascular endothelial cells was investigated by incubating endothelial cell cultures in the presence of 32p-labeled phosphoric acid. the incorporation of 32p into proteins was measured after fractionation by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and autoradiography of the gel. in endothelial cells seeded at low density and made quiescent by serum s ... | 1986 | 3486870 |
| effect of transcutaneous electrical nerve stimulation on pain perception threshold and pain tolerance level of human teeth subjected to electrical stimulation. | | 1986 | 3488206 |
| percutaneous intracardiac direct-current shocks in dogs: arrhythmogenic potential and pathological changes. | in 12 dogs, a total of 65 direct-current (dc) shocks of 100-300 j were delivered through a standard usci6f tripolar electrode catheter to selected sites in the heart. severe arrhythmias were more frequent after electric shocks of high energy to the ventricles and av-nodal or his-bundle region than after comparable shocks to the left or right atria. there was a direct relationship between the strength of the electric shocks, and the extent and severity of the injury. application of 300 j shocks l ... | 1986 | 3488215 |
| treatment of cutaneous sclerosis and aplastic anemia with antithymocyte globulin. | we administered antithymocyte globulin to a 52-year-old man with cutaneous sclerosis (scleroderma) and severe aplastic anemia for treatment of the aplastic lesion. the use of this therapy resulted not only in marrow recovery but also in resolution of the sclerosis. this observation indicates that t lymphocytes, their products, or both, are important factors in the pathogenesis of cutaneous sclerosis. furthermore, the success of this treatment could play a role in future investigations involving ... | 1987 | 3491559 |
| binding of [3h]skf 38393 to dopamine d-1 receptors in rat striatum in vitro; estimation of receptor molecular size by radiation inactivation. | [3h]skf 38393 (2,3,4,5-tetrahydro-7,8-dihydroxy-1-phenyl-1h-3-benzazepine) binds with high affinity to 3,4-dihydroxyphenylethylamine (dopamine) d-1 receptors in rat striatum in vitro (kd = 7 and 14 nm in nonfrozen and frozen striatum, respectively). the number of binding sites (bmax) was approximately 80.0 pmol/g of original tissue, a value similar to the bmax for the dopamine d-1 antagonist sch 23390. nondisplaceable [3h]skf 38393 binding was approximately 45% of total binding. irradiation (0-4 ... | 1987 | 3491877 |
| application of enzyme immunoassays for the confirmation of clinically suspect plague in namibia, 1982. | an outbreak of plague occurred in ovamboland, northern namibia, late in 1982. blood cultures, sera and blood clots were tested to obtain laboratory confirmations for clinically suspect cases of the disease. isolation of the bacillus (yersinia pestis) was attempted from blood cultures; sera were tested for antibody by passive haemagglutination (pha) and enzyme-linked immunosorbent assay (elisa). sera and clots also were tested by elisa for the specific f1 plague antigen. all the elisa procedures ... | 1986 | 3492308 |
| sharing of mhc haplotypes among apparently unrelated patients with congenital adrenal hyperplasia due to 21-hydroxylase deficiency. | from the study of hla, complement, and glyoxalase i alleles in 82 venezuelan individuals belonging to 19 families of mixed ethnic origin having 20 affected newborns with salt-wasting congenital adrenal hyperplasia due to 21-hydroxylase (21-oh) deficiency, a total of 38 disease haplotypes and 53 nondisease haplotypes were found. of the pathological haplotypes 47% were found to share the hla-b39 or -bw62 specificities, 55% of them in combination with the bfs, c2c, c4a4, c4b2 (sc42) complotype. the ... | 1987 | 3493216 |
| yersinia pestis endophthalmitis. | | 1987 | 3495184 |
| dexamethasone pre-treatment is antipyretic toward polyinosinic: polycytidylic acid, lipopolysaccharide and interleukin 1/endogenous pyrogen. | the effect of intravenously injected dexamethasone on the febrile response of rabbits to polyinosinic: polycytidylic acid (poly i:c), lipopolysaccharide (lps) and interleukin 1/endogenous pyrogen (il1/e.p.) was studied. dexamethasone (1 mg/kg) attenuated the febrile response to poly i:c (5 micrograms/kg) but only if administered between 0.5 to 2 h before poly i:c. if it was given after poly i:c this resulted in a potentiation of the fever. antagonism of the febrile response to poly i:c by dexame ... | 1987 | 3495738 |
| antigenic and amino acid sequence analysis of the variants of h1n1 influenza virus in 1986. | since their reintroduction to human populations in 1977, influenza a viruses of the h1n1 subtype have undergone antigenic drift. recently a distinct antigenic variant, a/singapore/6/86, has been almost exclusively isolated internationally, and the antigenic properties and amino acid sequence of its haemagglutinin have been determined and compared with those of the haemagglutinins of other h1n1 viruses, in particular a/chile/1/83. fourteen amino acid sequence differences are detected between the ... | 1987 | 3496983 |
| local cerebral metabolic effects of l-dopa therapy in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced parkinsonism in monkeys. | the quantitative 2-deoxy[14c]glucose autoradiographic method was used to map the distribution of alterations in local cerebral glucose utilization that accompanies clinically effective chronic l-dopa therapy of rhesus monkeys made parkinsonian by the administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (mptp). this pattern of changes was compared to the effects of a similar treatment regimen in normal monkeys. l-dopa (100 mg with 10 mg carbidopa) was administered orally to normal and p ... | 1987 | 3497401 |
| functional roles of human class ii antigens in t cell proliferative response. comparison of the dr and dq molecules using mouse monoclonal antibodies. | the monoclonal antibody (mab) nc1 recognizing a monomorphic dr determinant and the anti-dq mabs plm2, plm9, and plm12 recognizing polymorphic dq determinants were produced. nc1 reacted with the h1l1 (dr) and h1l2(drw52, drw53 and allelic products) complexes of a dr family, while the specificity of plm12 was dqw3 (previously called tb21). both plm2 and plm9 reacted with the allodeterminant of ta10 (a subtype of dqw3). these three mabs immunoprecipitated the same h2l3 complex of a dq family. with ... | 1986 | 3497988 |
| difference in monoamine oxidase b activity between c57 black and albino nmri mouse strains may explain differential effects of the neurotoxin mptp. | monoamine oxidase b (mao-b) is the key enzyme in the conversion of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(mptp) to n-methyl-4-phenyl-pyridinium ion (mpp+) which causes degeneration of dopaminergic nigral neurons. using a histochemical tetrazolium method for mao-b with tyramine as substrate and chlorgyline for the inhibition of mao-a, black c57 mice were found to have a higher brain mao-b activity than similar aged albino nmri mice. the difference, which was in general density rather than d ... | 1987 | 3498133 |
| salivary immunoreactive human epidermal growth factor (ir-hegf) in patients with peptic ulcer disease. | egf is reported to have a potent protective effect on peptic ulcer formation in rats. in this study, we measured the ir-hegf concentrations in the saliva of normal human subjects and patients with peptic ulcer disease or non-peptic ulcer gastroduodenal disease. in normal subjects, the level of salivary ir-hegf was highest in the early morning, and the values in individuals on different days showed small variations. there were no sex differences or age-related changes in the salivary ir-hegf leve ... | 1987 | 3499370 |
| vaccination against cutaneous leishmaniasis in a murine model. ii. immunologic properties of protective and nonprotective subfractions of soluble promastigote extract. | we have previously demonstrated that balb/c mice can be protected against a fatal infection with leishmania major by i.p. immunization with a soluble leishmanial antigen (sla) preparation in conjunction with the adjuvant, corynebacterium parvum (cp). in this study, sla was separated into nine distinct fractions by anion exchange liquid chromatography, and the fractions were analyzed for their ability to stimulate t cells obtained from immunized mice, to be recognized by vaccine-induced antibodie ... | 1987 | 3499465 |
| biphasic effects of epidermal growth factor on bone nodule formation by isolated rat calvaria cells in vitro. | osteoblast-like cells isolated from fetal rat calvaria (rc) form three-dimensional nodules in vitro having the morphological and immunohistochemical characteristics of bone. when administered continually over 21 days of culture, egf caused a dose-related inhibition of nodule formation, as well as a reduction in the proportion of mineralized nodules, at concentrations between 10(-12) to 10(-7) m. the same conditions caused an increase in both cell proliferation and saturation density in the cultu ... | 1987 | 3502681 |
| biphasic effects of epidermal growth factor on bone nodule formation by isolated rat calvaria cells in vitro. | osteoblast-like cells isolated from fetal rat calvaria (rc) form three-dimensional nodules in vitro having the morphological and immunohistochemical characteristics of bone. when administered continually over 21 days of culture, egf caused a dose-related inhibition of nodule formation, as well as a reduction in the proportion of mineralized nodules, at concentrations between 10(-12) to 10(-7) m. the same conditions caused an increase in both cell proliferation and saturation density in the cultu ... | 1987 | 3502681 |
| post-translational regulation of lcr plasmid-mediated peptides in pesticinogenic yersinia pestis. | the low calcium response of wild type yersinia pestis, the causative agent of bubonic plague, and of enteropathogenic yersinia pseudotuberculosis and yersinia enterocolitica is known to be mediated by a shared lcr plasmid of about 70 kb. at 37 degrees c in ca2+-deficient medium, this element promotes restriction of growth with concomitant production of virulence functions including the common v antigen and a set of yersiniae outer membrane peptides termed yops (lcr+). the latter are expressed by ... | 1987 | 3504547 |
| [a histopathological investigation of the effects of hypercupremy on bone healing]. | | 1986 | 3505435 |
| modulation of the low-calcium response in yersinia pestis via plasmid-plasmid interaction. | virulent cells of yersinia pestis, yersinia pseudotuberculosis, and yersinia enterocolitica are known to exhibit a low-calcium response in vitro characterized by restriction of growth and induction of v antigen at 37 degrees c in ca2+-deficient media (lcr+). a shared lcr plasmid mediates these properties and encodes yersiniae outer membrane peptides (yops) that are expressed in lcr+ y. pseudotuberculosis and y. enterocolitica but not y. pestis. we present direct evidence here verifying that synt ... | 1987 | 3507558 |
| [the utilization of iron of various substrates by yersinia pestis]. | | 1987 | 3508925 |
| the biochemical basis of niridazole toxicity. | | 1987 | 3509430 |
| induction and activity of several isoenzymes of cytochrome p-450 in primary cultures of rat hepatocytes, in comparison with in vivo data. | changes in cytochrome p-450 isoenzymes were studied in rat liver and in primary cultures of rat hepatocytes after treatment with compounds belonging to various classes of inducers, including phenobarbital (pb), beta-naphthoflavone (bnf), and clofibrate/clofibric acid (clof/clofa). the enzyme activity toward specific substrates was measured, and the presence of apoprotein of several p-450 isoenzymes was determined semiquantitatively by western blotting. in untreated cultures the p-450 content and ... | 1987 | 3509691 |
| prostate cancer--some aspects of epidemiology, risk factors, treatment and survival. | in western populations prostate cancer was probably rare in the past, yet in many populations the mortality rate has risen 3-5-fold since 1910. the disease now affects 1 man in 20. while the incidence in third world populations living traditionally remains low, it increases with urbanization and prosperity, as in south african blacks. while the disease is age-related, more common in married than in single men and family-orientated, knowledge of specific aetiological factors is meagre. abnormal h ... | 1986 | 3510459 |
| dog inactive renin: biochemical characterization and secretion into renal plasma and lymph. | it has been suggested that the dog is a useful model for studies of inactive renin (ir). however, the nature and origin of the trypsin-activated angiotensin-forming activity in dog plasma have not been fully defined. we characterized dog ir using renin-specific antibody, immunoaffinity, and affigel blue chromatography. activated ir resembles renal and plasma active renin in biochemical and immunological properties. ir was detected in renal lymph, arterial and venous plasma, as well as renal extr ... | 1986 | 3510560 |
| intestinal bacteria translocate into experimental intra-abdominal abscesses. | experimental intra-abdominal abscesses were initiated by surgical implantation of a fibrin clot contaminated with either bacteroides fragilis, bacteroides thetaiotaomicron, or b fragilis-escherichia coli. seven days after surgery the numbers of bacteroides (per gram) in b fragilis and b thetaiotaomicron abscesses were typically log10 8.4 +/- 0.5 (n = 6) and log10 6.4 +/- 0.6 (n = 4), respectively; b fragilis-e coli abscesses typically contained log10 8.9 +/- 0.5 b fragilis and log10 7.6 +/- 0.6 ... | 1986 | 3510604 |
| activation of the complement system by cryptococcus neoformans leads to binding of ic3b to the yeast. | the complement system plays a key role in resistance to cryptococcosis. in the present study, we examined several factors that influence the binding of c3 cleavage fragments to cryptococcus neoformans. binding of c3 was determined by using normal human serum supplemented with 125i-labeled c3. incubation of encapsulated cryptococci in 20% serum led to the binding of approximately 3.2 x 10(6) molecules of c3 to each cell. the binding of c3 was markedly inhibited by heating the serum at 56 degrees ... | 1986 | 3514450 |
| distribution of two urinary ribonuclease-like enzymes in human organs and body fluids. | in order to determine the distribution of two human urinary rnase (rnase us and rnase ul)-like enzymes in human tissues and body fluids, enzyme immunoassay systems were established using rabbit anti-rnase sera. the sensitivity of the assay systems was of similar order to that of radioimmunoassay systems previously reported. in the enzyme immunoassay, the cross reactivities of anti-rnase ul serum towards rnase us, bovine kidney rnase k2, bovine rnase a, and bovine seminal rnase vs were less than ... | 1986 | 3514589 |
| distribution of two urinary ribonuclease-like enzymes in human organs and body fluids. | in order to determine the distribution of two human urinary rnase (rnase us and rnase ul)-like enzymes in human tissues and body fluids, enzyme immunoassay systems were established using rabbit anti-rnase sera. the sensitivity of the assay systems was of similar order to that of radioimmunoassay systems previously reported. in the enzyme immunoassay, the cross reactivities of anti-rnase ul serum towards rnase us, bovine kidney rnase k2, bovine rnase a, and bovine seminal rnase vs were less than ... | 1986 | 3514589 |
| [kinetics of the immunologic agglutination reaction and express determination of bacteria using an automated laser photon-correlation spectrometer]. | | 1986 | 3516614 |
| growth-associated protein, gap-43, a polypeptide that is induced when neurons extend axons, is a component of growth cones and corresponds to pp46, a major polypeptide of a subcellular fraction enriched in growth cones. | growth-associated protein, gap-43, is a polypeptide that is induced in neurons when they grow axons. we show by means of subcellular fractionation and immunohistochemical localization that gap-43 is a component of neuronal growth cones as well as growing neurites; it is similar to a major phosphoprotein, pp46, of a growth cone-enriched subcellular fraction. these conclusions are consistent with the possibility that the induction of gap-43/pp46 is an important event in the establishment of a prod ... | 1986 | 3517863 |
| the distribution of vip-immunoreactive neurons in the visual cortex of adult rabbits and during postnatal development. | in the adult rabbit, the majority of the vip-immunoreactive neurons are distributed in layer ii-iii of the visual cortex. labeled cells are also seen in layers vi, iv, v and occasionally in layer i. during postnatal development, we can see labeled neurons on the 2nd day of life, and in layers ii-iii-iv-v-vi on the 4th day. on the 7th day, these neurons reach a distribution typical in the adult animal. from the 2nd to the 10th day the number of vip-neurons increases, and from the 10th day to matu ... | 1986 | 3518861 |
| divinylsulphone-activated agarose. formation of stable and non-leaking affinity matrices by immobilization of immunoglobulins and other proteins. | divinylsulphone-activated agarose is an attractive alternative to several of the activated supports usually used. unlike cnbr-activated gels, it does not leak the immobilized protein at high ph. it reacts readily with proteins at near-neutral ph (unlike the epoxy-activated supports). generally, divinylsulphone-activated agarose reacts with amino, hydroxyl, and sulphydryl groups, thus allowing immobilization of a wide spectrum of ligands. moreover, it is available in an aqueous suspension free of ... | 1986 | 3519637 |
| plasmids and pestilence--biological and clinical aspects of bubonic plague. | | 1986 | 3521095 |
| altered estrogen action in the senescent rat uterus: a model for steroid resistance during aging. | the estrogen stimulated rat uterus serves as a useful model to examine steroid resistance during aging. this system exhibits receptor loss, impaired stimulation of rna polymerase ii and defects in nuclear translocation (or enhanced association) of receptor-estradiol complexes. all of these defects appear to contribute in part to decreased estrogen responsiveness of the senescent rodent uterus. | 1986 | 3521224 |
| [mathematical modelling of the interaction of the plague capsular antigen with antibodies of varying affinity]. | the group of high affinity antibodies is advantageous in antigen binding because of different dissociation rates of the antigen-antibody complexes. an antigen interacts with such a group in accordance with the value of the parameters characterizing the group and the antigen. the unbound antigen interacts with the subsequent group by the affinity value and so on. this is the basis of the presented model of univalent antigen interaction with antibodies of different affinity. the model values of th ... | 1986 | 3521467 |
| formation of behaviorally active estrogen in the dove brain: induction of preoptic aromatase by intracranial testosterone. | the preoptic area (poa) of the male ring dove is a target for specific behavioral effects of estrogen that are separable from those of androgen. activity of the aromatase system in the poa, which converts testosterone to 17 beta-estradiol (e2), is increased by systemic androgen. using crystalline steroid implants positioned stereotaxically in the brain, we examined whether aromatase induction is a result of direct steroid action on the poa, which can occur independently of the behavioral effects ... | 1986 | 3526169 |
| formation of behaviorally active estrogen in the dove brain: induction of preoptic aromatase by intracranial testosterone. | the preoptic area (poa) of the male ring dove is a target for specific behavioral effects of estrogen that are separable from those of androgen. activity of the aromatase system in the poa, which converts testosterone to 17 beta-estradiol (e2), is increased by systemic androgen. using crystalline steroid implants positioned stereotaxically in the brain, we examined whether aromatase induction is a result of direct steroid action on the poa, which can occur independently of the behavioral effects ... | 1986 | 3526169 |
| evolution of a model of estrogen action. | | 1986 | 3526452 |
| an escherichia coli rna polymerase tight-binding site on t7 dna is a weak promoter subject to substrate inhibition. | a specific escherichia coli rna polymerase tight-binding (tb) site on bacteriophage t7 has been located at 32,988 base pairs from the left end of t7. this site is referred to as the t7 f promoter since it is fully active in vitro. kinetics of association and dissociation have been measured by use of the abortive initiation assay and runoff transcription. the association constant, ka approximately 9 x 10(5) m-1 s-1, is of the same magnitude as ka for the t7 minor promoters. in competitive titrati ... | 1986 | 3535875 |
| pharmacokinetics of insulin following intravenous and subcutaneous administration in canines. | studies were conducted to examine the absorption and disposition kinetics of insulin in dogs following intravenous (iv) and subcutaneous (sc) administration of commercial preparations. after iv and sc dosing, the plasma levels were described by models which considered basal insulin level contributions. intersubject variation in the disposition kinetics was small with half-lives of 0.52 +/- 0.05 h and total body clearances of 16.21 +/- 2.08 ml min-1 kg-1. calculated insulin plasma secretion rates ... | 1986 | 3535917 |
| chlamydial infections. | | 1986 | 3536193 |
| studies on the mechanics of the normal human middle ear. | the middle ear was studied in temporal bone preparations using a laser-doppler interferometer. for measurements at a sound level of 80 db spl this method proved to be very reliable, as was shown by good reproducibility of results in experiments over more than 6 hours. the vibrations of the tympanic membrane and stapes footplate were studied from 200 hz to 10 khz and the results demonstrate a piston-like movement of the stapes footplate up to 120 db spl. the damping effect of the normal ear is lo ... | 1986 | 3536194 |
| agglutination and mating activity of the mf alpha 2-encoded alpha-factor analog in saccharomyces cerevisiae. | the mf alpha 2-encoded asn-5,arg-7 alpha-factor-like peptide has been shown shown to have similar activity to gln-5,lys-7 alpha-factor in morphogenesis and growth arrest studies (s. raths, p. shenbagamurthi, f. naider, and j. m. becker, j. bacteriol. 168:1468-1471, 1986). we tested the asn-5,arg-7 peptide in agglutination and mating assays and found that its activity was similar to or slightly less than that of the gln-5,lys-7 alpha-factor. the asn-5,arg-7 alpha-factor-like peptide is thus the m ... | 1986 | 3536884 |
| agglutination and mating activity of the mf alpha 2-encoded alpha-factor analog in saccharomyces cerevisiae. | the mf alpha 2-encoded asn-5,arg-7 alpha-factor-like peptide has been shown shown to have similar activity to gln-5,lys-7 alpha-factor in morphogenesis and growth arrest studies (s. raths, p. shenbagamurthi, f. naider, and j. m. becker, j. bacteriol. 168:1468-1471, 1986). we tested the asn-5,arg-7 peptide in agglutination and mating assays and found that its activity was similar to or slightly less than that of the gln-5,lys-7 alpha-factor. the asn-5,arg-7 alpha-factor-like peptide is thus the m ... | 1986 | 3536884 |
| [plasmids of the pathogenicity of yersinia pestis]. | | 1986 | 3537763 |
| uromodulin, an immunosuppressive protein derived from pregnancy urine, is an inhibitor of interleukin 1. | uromodulin, an 85-kda glycoprotein isolated from pregnancy urine, has been shown to inhibit antigen-induced proliferation of human lymphocytes in vitro. the present investigation was undertaken to determine its mechanism of action. uromodulin was found to be a potent inhibitor of interleukin 1 (il-1)-induced thymocyte proliferation. uromodulin was compared to a previously described 30- to 35-kda il-1 inhibitor isolated from urine of febrile patients (febrile inhibitor). uromodulin and the febril ... | 1986 | 3538018 |
| uromodulin, an immunosuppressive protein derived from pregnancy urine, is an inhibitor of interleukin 1. | uromodulin, an 85-kda glycoprotein isolated from pregnancy urine, has been shown to inhibit antigen-induced proliferation of human lymphocytes in vitro. the present investigation was undertaken to determine its mechanism of action. uromodulin was found to be a potent inhibitor of interleukin 1 (il-1)-induced thymocyte proliferation. uromodulin was compared to a previously described 30- to 35-kda il-1 inhibitor isolated from urine of febrile patients (febrile inhibitor). uromodulin and the febril ... | 1986 | 3538018 |
| antibacterial activity of amniotic fluid in the early third trimester. its association with preterm labor and delivery. | the purpose of this study was to determine the bacterial growth inhibitory property of amniotic fluid (af) from patients in the early third trimester and to examine its relationship to preterm labor and delivery. a total of 38 af samples from patients between 27 and 35 weeks gestation with intact membranes were studied. antibacterial activity was tested against escherichia coli, staphylococcus aureus, and bacteroides fragilis. fifteen fluids were from patients in preterm labor who delivered near ... | 1987 | 3539135 |
| [effects of acute hypoxemia on fetal movement in the fetal lamb]. | chronically instrumented pregnant models were established using 5 dorset-rambouillet pregnant ewes with gestational age between 120 and 138 days. observation of fetal movements were started on the 3rd postoperative day or later when recovery from the surgery to the physiological condition was confirmed by maternal and fetal biophysical parameters. fetal movements were observed using real-time ultrasound equipment with linear-array transducer placed on the maternal abdomen. observation encompasse ... | 1986 | 3543167 |
| a cytosolic protein contains a cryptic mitochondrial targeting signal. | cytosolic dihydrofolate reductase from mouse contains a cryptic mitochondrial targeting sequence. if this sequence is attached to the amino terminus of 'passenger' proteins which by themselves cannot enter mitochondria, the resulting fusion proteins are transported into yeast mitochondria. | 1987 | 3543689 |
| [evaluation of vaccinal immunogenesis by the peroxidase method]. | the possibility of evaluating functional immunomorphogenesis in the course of the vaccinal process after the injection of conjugated and live brucellosis vaccines, as well as conjugated plague antigen and yersinia pestis strain ev, to guinea pigs has been shown by means of the direct and two-layer variants of the immunoperoxidase assay. the dynamics of the accumulation of globulin-producing cells in the immunocompetent organs and the time course of immunoglobulin titers in the peripheral blood a ... | 1986 | 3548171 |
| nocardia asteroides as a cause of corneal ulcer. case report. | | 1987 | 3551895 |
| elimination of 131i in meadow voles (microtus pennsylvanicus) measured by whole-body gamma spectrometry. | | 1987 | 3553074 |
| [pathogenic properties of yersinia and their role in yersiniosis pathology]. | | 1987 | 3554847 |
| rhodopsin, vitamin a, and interstitial retinol-binding protein in the rd chicken. | in order to determine whether blindness in the rd strain of rhode island red chickens is due to a defect in the vitamin a (visual) cycle, spectroscopy, high performance liquid chromatography, and immunochemical techniques were used to compare the amounts of rhodopsin, interstitial retinol-binding protein, and vitamin a compounds in the dark-adapted eyes of homozygous rd and heterozygous carriers. in both groups of chickens, (up to 6 weeks post-hatching) the distribution of stored vitamin a diffe ... | 1987 | 3557867 |
| phase dynamics at microtubule ends: the coexistence of microtubule length changes and treadmilling. | the length dynamics both of microtubule-associated protein (map)-rich and map-depleted bovine brain microtubules were examined at polymer mass steady state. in both preparations, the microtubules exhibited length redistributions shortly after polymer mass steady state was attained. with time, however, both populations relaxed to a state in which no further changes in length distributions could be detected. shearing the microtubules or diluting the microtubule suspensions transiently increased th ... | 1987 | 3558477 |
| cell length to diameter relation of rat fetal radial glia--does impaired k+ transport capacity of long thin cells cause their perinatal transformation into multipolar astrocytes? | in thick sections of golgi-impregnated late fetal rat brains, radial glial cells were measured for both length and diameter of their main (basal) processes. the process diameter was found to decrease proportionally to the square root of cell length; thus, the cytoplasm volume remained fairly constant for cells in the range of lengths studied (100-2500 microns). the measured data were used for calculation of the cell's space constant lambda in order to estimate their capability to carry spatial b ... | 1987 | 3561861 |
| prevention of adolescent chemical dependency. | chemical dependency is part of a significant pattern of serious health problems resulting from the breakdown of social control over impulsive, pleasure-driven adolescent behavior. the drug epidemic in north america, after two decades of relentless increases, shows encouraging signs that stopping it may be possible. chemical dependency prevention became a serious concern in the 1970s in response to the rising drug epidemic. initial efforts focused on drug-specific education in the mass media and ... | 1987 | 3562105 |
| co-dergocrine, cerebral glucose utilization and maze performance in middle-aged rats. | the objective of this study was to determine the effect of co-dergocrine in rats on local cerebral glucose utilization and performance in a complex t-maze. middle-aged (12-16 months) male fischer-344 rats were given injections of co-dergocrine (3 or 10 mg/kg, ip) 35 min before behavioral testing or the administration of 2-deoxy-d-[1-14c]glucose ([14c]dg), a radiotracer for local cerebral glucose utilization (lcgu). both doses stimulated lcgu in the locus ceruleus and median raphe nucleus and in ... | 1987 | 3562504 |
| effects of perfluorodecanoic acid on thyroid status in rats. | treatment of rats with toxic doses of perfluorodecanoic acid (pfda) results in reduction in feed intake, body weight, serum thyroxine (t4) and triiodothyronine (t3) concentrations, resting heart rates, and body temperatures. some of these effects resemble changes characteristic of hypothyroidism. therefore the effects of pfda on functional thyroid status were examined to relate changes in thyroid status with signs of pfda toxicity. in the present study, the dose-related effects of pfda on plasma ... | 1987 | 3564018 |
| effects of perfluorodecanoic acid on thyroid status in rats. | treatment of rats with toxic doses of perfluorodecanoic acid (pfda) results in reduction in feed intake, body weight, serum thyroxine (t4) and triiodothyronine (t3) concentrations, resting heart rates, and body temperatures. some of these effects resemble changes characteristic of hypothyroidism. therefore the effects of pfda on functional thyroid status were examined to relate changes in thyroid status with signs of pfda toxicity. in the present study, the dose-related effects of pfda on plasma ... | 1987 | 3564018 |
| effect of the virulence associated 47 megadalton plasmid of yersinia pestis on permeability to gentian violet and sensitivity to novobiocin. | the loss of the 47 megadalton (mdal) plasmid of yersinia pestis resulted in increased gentian violet uptake and increased sensitivity to novobiocin. the addition of calcium caused a decrease in gentian violet uptake and a decrease in novobiocin sensitivity independent of the plasmid. differentiation of cells bearing and lacking the plasmid was accomplished using novobiocin in the presence of calcium at 26 degrees c. the novobiocin resistance levels of cells lacking the 47 mdal plasmid and cells ... | 1986 | 3564897 |
| a permeability mutant of yersinia pestis with increased susceptibility to phagocytosis which retains potential for intraphagocytic growth and virulence. | gentian violet resistance was used to select mutants of yersinia pestis with altered cell envelope permeability. mutants in one class lacked the 6 and 61 megadalton (mdal) plasmids, but retained the 47 mdal plasmid associated with calcium dependence. mutants in a second class retained all three plasmids. one mutant in the latter class, ev76s7, lacked major outer membrane protein j and yielded reduced levels of minor outer membrane proteins a and c. protein j is known to interact differently with ... | 1986 | 3564898 |
| the calcium dependence region of the 47 megadalton plasmid of yersinia pestis is required for growth within macrophages. | yersinia pestis cells bearing the 6,47 and 61 megadalton (mdal) plasmids have been shown previously to be more resistant to novobiocin and to take up less gentian violet than cells lacking the 47 mdal plasmid. mutants with lesions in the calcium dependence region of the 47 mdal plasmid, which are known to have resistance to high levels of novobiocin, were found to have rates of gentian violet and deoxycholate sensitivities identical to cells with intact plasmids. cells bearing the 6, 47 and 61 m ... | 1986 | 3564899 |
| an examination of ciliary neuronotrophic factors from avian and rodent tissue extracts using a blot and culture technique. | we have previously reported a technique for determining the apparent molecular weight (mr) of ciliary neuronotrophic factors (cntfs) in crude extracts. this method involves sds-polyacrylamide gel electrophoresis of the extract. western blotting and culture of purified ciliary ganglion neurons on the paper containing the blotted lane. neurons will survive only if in direct contact with the trophic factor band and the surviving neurons, when stained with a vital dye, will outline the cntf band the ... | 1987 | 3567656 |
| an examination of ciliary neuronotrophic factors from avian and rodent tissue extracts using a blot and culture technique. | we have previously reported a technique for determining the apparent molecular weight (mr) of ciliary neuronotrophic factors (cntfs) in crude extracts. this method involves sds-polyacrylamide gel electrophoresis of the extract. western blotting and culture of purified ciliary ganglion neurons on the paper containing the blotted lane. neurons will survive only if in direct contact with the trophic factor band and the surviving neurons, when stained with a vital dye, will outline the cntf band the ... | 1987 | 3567656 |
| effects of d,l-propranolol on apomorphine induced stereotyped behavior in rats. | the effects of d,l propranolol on stereotyped behavior induced by apomorphine administration was studied quantitatively. d,l propranolol caused a 2.5 leftward displacement of the control dose-response curve constructed to apomorphine-induced stereotypy; the ed50 for apomorphine was reduced from 1.87 +/- 0.38 to 0.74 +/- 0.17. the potentiation was dependent on both d,l-propranolol and apomorphine doses. results are discussed in the light of a possible interference of the central noradrenergic sys ... | 1987 | 3569842 |
| [combined allergic effects of volatile complexes of epoxy and phenolformaldehyde compositions]. | | 1987 | 3570013 |
| [combined allergic effects of volatile complexes of epoxy and phenolformaldehyde compositions]. | | 1987 | 3570013 |
| involvement of gastrointestinal mechano- and intestinal chemoreceptors in vagal reflexes: an electrophysiological study. | the role of gastrointestinal mechanoreceptors and intestinal chemoreceptors in the genesis of vago-vagal reflexes was assessed by recording single vagal efferent fibre discharge in the urethane-anaesthetized ferret during procedures known to activate discrete populations of gastrointestinal afferent fibres. distension of the stomach, duodenum and jejunum was used to activate mechanoreceptors while perfusion of the intestinal loops with various chemical solutions was used to activate mucosal chem ... | 1987 | 3571829 |
| anticholinesterase effect and toxicity of bis(trichloromethyl) sulfone (n-1386 biocide) in rats. | the oral ld50 for bis(trichloromethyl) sulfone (n-1386 biocide) in male rats was 691 mg/kg. deaths occurred 1-5 days after treatment and signs of toxicity suggestive of an anticholinesterase effect were noted. however, neither plasma cholinesterase nor brain acetylcholinesterase was inhibited 2, 4 or 24 hours after a single, oral dose of 500 mg/kg. atropine (300 mg/kg, s.c.) or scopolamine (670 mg/kg, s.c.) pretreatments did not protect against the acute lethality of bis(trichloromethyl) sulfone ... | 1987 | 3573978 |
| [various physico-chemical properties of the yersinia pestis capsular protein]. | some properties of the structure of y. pestis capsular antigen macromolecules have been studied. the aminoacid composition of f1 protein, the aminoacid sequence of the n-terminal fragment of antigen polipeptide chain were determined. some peculiarities in the dissociation of capsular antigen macromolecules have been studied. the formation of the product resulting from unterminated thermodissociation of f1 protein oligomeric form, consisting of four subunits, has been registered. the aspects of f ... | 1987 | 3574316 |
| fear-potentiated startle using an auditory conditioned stimulus: effect of lesions of the amygdala. | the effect of lesions of the amygdala on fear-potentiated startle using an auditory conditioned stimulus (cs) was evaluated, after replicating and extending previous findings that a tone is an effective cs for fear-potentiated startle. rats received 10 tone-shock pairings on 2 successive days. at 24-48 hr following training, they received bilateral electrolytic lesions of the central nucleus of the amygdala or sham operations, and then were tested for fear-potentiated startle 4-5 days later. les ... | 1987 | 3575483 |
| [reflection of the effect of binaural release from masking in human long-latency auditory evoked potentials]. | | 1987 | 3582841 |
| [reflection of the effect of binaural release from masking in human long-latency auditory evoked potentials]. | | 1987 | 3582841 |
| lysosomal membrane stabilization by enfenamic acid. | | 1986 | 3583334 |
| an enzyme-linked immunosorbent assay to detect serum igg to pasteurella multocida in naturally and experimentally infected rabbits. | an enzyme-linked immunosorbent assay (elisa) was evaluated for efficacy in detecting serum igg against pasteurella multocida in both naturally and experimentally infected rabbits. blood samples and nasal cultures were taken concurrently from 58 rabbits from four conventional rabbitries. nine rabbits from a pasteurella-free colony served as negative controls. fifty-six rabbits were elisa positive. of these, 46 were p. multocida culture positive, 10 were culture negative. two rabbits were elisa ne ... | 1987 | 3586606 |
| an enzyme-linked immunosorbent assay to detect serum igg to pasteurella multocida in naturally and experimentally infected rabbits. | an enzyme-linked immunosorbent assay (elisa) was evaluated for efficacy in detecting serum igg against pasteurella multocida in both naturally and experimentally infected rabbits. blood samples and nasal cultures were taken concurrently from 58 rabbits from four conventional rabbitries. nine rabbits from a pasteurella-free colony served as negative controls. fifty-six rabbits were elisa positive. of these, 46 were p. multocida culture positive, 10 were culture negative. two rabbits were elisa ne ... | 1987 | 3586606 |
| the blocking of human antibody-dependent, eosinophil-mediated killing of schistosoma mansoni schistosomula by monoclonal antibodies which cross-react with a polysaccharide-containing egg antigen. | three igm monoclonal antibodies, m22g11p, m7b7 and m22p3g, which reacted with the surface of schistosoma mansoni schistosomula in an indirect fluorescent antibody assay, were found to recognize a polysaccharide-containing egg antigen previously designated k3. the monoclonal antibodies and a monospecific rabbit anti-k3 serum also recognized a cross-reacting antigen in a crude cercarial antigen preparation. in an eosinophil-mediated schistosomulum killing assay, all three monoclonal antibodies sig ... | 1987 | 3588013 |
| [role of iron in yersinia pestis multiplication in normal mammalian blood sera]. | y. pestis multiply in normal blood sera of susceptible and nonsusceptible animals at 28 degrees c, while at 37 degrees c bacteriostasis has been registered. inhibition of the growth of y. pestis at this temperature is due to the limited supply of iron to bacterial cells. | 1987 | 3591122 |
| cardiac-specific troponin-i radioimmunoassay in the diagnosis of acute myocardial infarction. | the cardiac isotype of the myofibrillar contractile protein, troponin-i, is located specifically in the mammalian heart. a sensitive radioimmunoassay has been developed to detect human and nonhuman primate cardiac troponin-i in serum down to 10 ng/ml. immunochemical cross reactivity with skeletal troponin-i was only 2% and was species nonspecific. normal patient levels of cardiac troponin-i are about 10 ng/ml. in patients with acute myocardial infarction (n = 32), serum cardiac troponin-i was el ... | 1987 | 3591601 |
| pulsatile release of antipyretic neuropeptide alpha-msh from septum of rabbit during fever. | the effects on fever of central administration of alpha-melanocyte-stimulating hormone (alpha-msh) and its antiserum, the specific increase in central concentration of alpha-msh during fever, and related results indicate that this neuropeptide is important to central nervous system (cns) regulation of the febrile response. the present experiments were designed to establish whether alpha-msh is released from septal tissue during fever and, if so, in what temporal course relative to the febrile re ... | 1987 | 3591986 |
| flow injection procedures for the determination of ethanol and alcohol dehydrogenase using co-immobilised bacterial luciferase and oxidoreductase. | | 1987 | 3592249 |
| effect of postmortem factors on muscarinic receptor subtypes in rat brain. | although prior studies have supported the validity of measuring total muscarinic receptor binding in postmortem brain, there has not been a study of postmortem effects on muscarinic receptor subtypes, m1 and m2, defined by high and low affinity for pirenzepine, respectively. we have examined in rat brain the effect of postmortem delay at room temperature, storage at 4 degrees c and -20 degrees c, and multiple freeze/thaw cycles on total muscarinic binding, measured with [3h]quinuclidinylbenzilat ... | 1987 | 3598588 |
| plasmid-mediated trimethoprim-resistance in staphylococcus aureus. characterization of the first gram-positive plasmid dihydrofolate reductase (type s1). | the trimethoprim-resistance gene located on plasmid psk1, originally identified in a multi-resistant staphylococcus aureus from australia, encodes the production of a dihydrofolate reductase (type s1), which confers a high degree of resistance to its host and is quite unlike any plasmid-encoded dihydrofolate reductase hitherto described. it has a low mr (19,700) and has a higher specific activity than the constitutive gram-negative plasmid dihydrofolate reductases. the type s1 enzyme is heat-sta ... | 1987 | 3606580 |
| effect of prolonged physical exercise on muscular phospholipase a2 activity in rats. | prolonged muscular exercise stimulates glucose uptake by the working muscles themselves. the mechanism of this phenomenon is at present unclear. it has been proposed that the kallikrein-kinin-prostaglandin system plays a role in the physiological regulation of muscular glucose metabolism during exercise. since bradykinin can stimulate phospholipase a2, a key enzymatic step in prostaglandin synthesis, phospholipase a2 activity was assayed in rats at rest and in rats compelled to swim for 60 minut ... | 1987 | 3609419 |
| [efficiency of neopsylla abagaitui fleas as plague vectors in transbaikalia]. | experiments have shown that the block of proventriculus develops in 1.1 to 1.5% of individuals of the flea neopsylla abagaitui infected with plague microbe. these insects transmit the agent during bloodsucking to different plague carriers (citellus dauricus, c. undulatus, meriones unguiculatus, microtus gregalis). the plague microbe is preserved in fleas for 65 days (the observation period). | 1987 | 3614985 |
| some newer imidazolones and their anti-inflammatory activity. | | 1987 | 3615561 |
| [acid-base equilibrium in calves with enteritis]. | studied were the indices of the acid-alkaline balance and the lactate level in a total of 19 black-and-white calves manifesting clinical forms of enteritis as well as in 10 clinically normal calves that belonged to the same breed. in most of the calves affected with enteritis the blood ph values proved essentially lower than those found in normal calves. differences were established between the normal and the diseased animals with regard to abe, hco-3, and pco2, the abe values with some of the a ... | 1987 | 3617465 |
| effects of local anesthetics on the passive permeability of sarcoplasmic reticulum vesicles to ca2+ and mg2+. | sarcoplasmic reticulum vesicles are used here as model membrane system to question the hypothesis of enhancement of permeability of cations by anesthetics, particularly that of ca2+ and of mg2+. the effects of dibucaine (up to 800 microm), tetracaine (up to 2 mm), lidocaine (up to 10 mm) and procaine (up to 10 mm) on the permeability of these membranes to ca2+ and mg2+ have been measured. we have used an experimental approach based on the light scattering method (kometani, t. and kasai, m. (1978 ... | 1987 | 3620467 |
| pattern-recognition program for analysis of colon myoelectric and pressure data. | a pattern-recognition program was developed which emulates visual scoring of colonic myoelectric and pressure recordings. it smoothes digitized data with a moving average filter, computes difference scores between successive groups of three data points, and uses the signs of these difference scores to detect the beginning and end of waves. adjacent waves are merged if their means are closer than 1.67 times the sum of their standard deviations, and amplitude and duration criteria are used to excl ... | 1987 | 3622189 |
| the contribution of afferent information on position and velocity to the control of slow and fast human forearm movements. | we applied vibration at various rates to the biceps tendon of a passive, restrained arm in normal human subjects and measured its effect on the perception of forearm position and the perception of forearm velocity. the disturbances of limb position perception and limb velocity perception depended on the vibration rate in distinctly different ways. we thereupon applied vibration at various rates to the biceps tendon during the performances of non-visually-guided slow and fast forearm movements. t ... | 1987 | 3622680 |