| increased virulence of yersinia pseudotuberculosis by two independent mutations. | a chromosomally encoded protein, which mediates invasion into hela cells was recently identified in yersinia pseudotuberculosis. the role of this protein (invasin) in the virulence process was not, however, investigated. we show that mutation of the invasin gene in y. pseudotuberculosis abolishes the ability of the bacteria to invade hela cells. when mice were challenged by intraperitoneal injection both the mutant and the wild-type strain produced infections of similar virulence but mutant show ... | 1988 | 3043229 |
| [use of quantitative immunofluorescence for determining the adsorption capacity of magnetic immunosorbents]. | the adsorption capacity of microgranulated polyacrylamide magnetic immunosorbents has been studied by the method of quantitative immunofluorescence as applied to the causative agents of plague, cholera, and melioidosis. similar regularity in the dynamics of antigen adsorption on the granules of magnetic immunoadsorbents has been established. this regularity consists in the direct relationship between this process and the concentration of infective agents interacting with magnetic sorbents. | 1988 | 3046196 |
| in vitro expression of a 22-kilodalton yersinia pestis polypeptide immunologically related to the 25-kilodalton plasmid-encoded protein of the three pathogenic yersinia species. | antibodies raised against the 25-kilodalton (p25) plasmid-encoded polypeptide of yersinia enterocolitica recognized the homologous protein in the three yersinia species grown in vitro. this polypeptide was recovered from whole cells as well as from the fluid supernatant of bacteria grown at 37 degrees c in a ca2+-deficient medium. furthermore, a 22-kilodalton (p22) plasmid-encoded polypeptide immunologically related to p25 was found only in y. pestis during early growth. after 30 h of culture, t ... | 1988 | 3047061 |
| immunoreactivity of a surface wall fraction produced by spherules of coccidioides immitis. | the membranous spherule outer wall (sow) isolated from liquid cultures of coccidioides immitis has been shown to elicit reactivity with human anti-coccidioides antibody by immunofluorescence and the immunodiffusion-tube precipitin assay. the serologically reactive components were extracted from sow with the nonionic detergent n-octyl-beta-d-glucopyranoside (og). the og-soluble fraction of sow was shown to be reactive with immunoglobulin g in 25 serum samples from coccidioidomycosis patients by a ... | 1988 | 3047063 |
| immunoreactivity of a surface wall fraction produced by spherules of coccidioides immitis. | the membranous spherule outer wall (sow) isolated from liquid cultures of coccidioides immitis has been shown to elicit reactivity with human anti-coccidioides antibody by immunofluorescence and the immunodiffusion-tube precipitin assay. the serologically reactive components were extracted from sow with the nonionic detergent n-octyl-beta-d-glucopyranoside (og). the og-soluble fraction of sow was shown to be reactive with immunoglobulin g in 25 serum samples from coccidioidomycosis patients by a ... | 1988 | 3047063 |
| effects of estrogen on hypothalamic gonadotropin-releasing hormone release in castrated male rhesus macaques. | the evidence that hypothalamic gonadotropin-releasing hormone (gnrh) release increases during the estrogen-induced luteinizing hormone (lh) surge in castrated female macaques and that estrogen induces a similar lh surge in the male suggests that either sexual differentiation of gnrh secretion does not exist or that changes in brain gnrh are not critical for ovulation. we tested this hypothesis by using the push-pull perfusion (ppp) technique to monitor gnrh release in the mediobasal hypothalamus ... | 1988 | 3048539 |
| in vitro and ex vivo enhancement of nonspecific phagocytosis by cefodizime. | the in vitro and ex vivo effects of cefodizime on some functional activities of both human neutrophils and monocytes were studied. in vitro experiments were performed with antibiotic concentrations ranging from 1 to 200 micrograms/ml. for the ex vivo study, 7 adult healthy controls were treated intravenously with 4 g/day of cefodizime for 6 days. we found that the drug modulated phagocytosis frequency and index when nonopsonized zymosan and heat-killed candida albicans were used as phagocytic ch ... | 1988 | 3053065 |
| in vitro and ex vivo enhancement of nonspecific phagocytosis by cefodizime. | the in vitro and ex vivo effects of cefodizime on some functional activities of both human neutrophils and monocytes were studied. in vitro experiments were performed with antibiotic concentrations ranging from 1 to 200 micrograms/ml. for the ex vivo study, 7 adult healthy controls were treated intravenously with 4 g/day of cefodizime for 6 days. we found that the drug modulated phagocytosis frequency and index when nonopsonized zymosan and heat-killed candida albicans were used as phagocytic ch ... | 1988 | 3053065 |
| the plasmid-encoded outer-membrane proteins of yersinia pestis. | the yersinial plasmid-encoded outer-membrane proteins (yops) are encoded by the low-ca++ response virulence plasmid present in the yersiniae pathogenic for humans. it has been shown that in yersinia pestis kim there are 11 of these proteins, which are expressed maximally during growth at 37 degrees c in the absence of ca++. to recover these proteins in outer membranes, it was necessary to transfer the y. pestis low-ca++ response plasmid to yersinia pseudotuberculosis. however, two of these prote ... | 1988 | 3055200 |
| the bubonic plague. | in a.d. 1346 some 100 million people inhabited europe, northern africa and the near east. five years later 25 million were dead--victims of the black death. the plague kept reappearing, but the epidemics did not spread as widely: apparently a new and milder strain of yersinia pestis evolved that made at least some people immune to the virulent strain. | 1988 | 3055286 |
| immunomodulators. | a new era is emerging in the therapy of rheumatic disease involving the use of biologically based immunomodulators. cytokines, such as the interleukins and interferons, are proteins produced by activated lymphoid cells which can now be synthesised by genetic engineering techniques. these agents have already been used to correct "immune deficiencies" in patients with neoplastic and inflammatory disease e.g. gamma-interferon in rheumatoid arthritis (ra). monoclonal antibodies (mca) targeted agains ... | 1988 | 3055878 |
| d-arabinose metabolism in escherichia coli b: induction and cotransductional mapping of the l-fucose-d-arabinose pathway enzymes. | d-arabinose is degraded by escherichia coli b via some of the l-fucose pathway enzymes and a d-ribulokinase which is distinct from the l-fuculokinase of the l-fucose pathway. we found that l-fucose and d-arabinose acted as the apparent inducers of the enzymes needed for their degradation. these enzymes, including d-ribulokinase, appeared to be coordinately regulated, and mutants which constitutively synthesized the l-fucose enzymes also constitutively synthesized d-ribulokinase. in contrast to d ... | 1988 | 3056899 |
| [comparative study of ribosomal proteins from yersinia pestis and escherichia coli: amino acid composition and electrophoretic mobility]. | the techniques of electrophoresis in polyacrylamide gel and amino acid analysis permitted to find the slight difference in the composition of ribosomal proteins from yersinia pestis and escherichia coli cells. ribosomal proteins were mapped and classified on the basis of two-dimensional electrophoresis data. protein "x" was registered in the total ribosomal protein due to its separation in course of ribosomal subunits dissociation. | 1988 | 3057358 |
| infection as a complication of heart transplantation. | infection and cardiac rejection are the most significant causes of morbidity and mortality after heart transplantation. at some transplant centers, more than half of the early transplantation-related deaths are the result of infection. the infectious agents may be transmitted to the host by means of the allograft, through blood transfusion, by nosocomial or environmental routes, or they may represent endogenous microbial flora or reactivation of a prior infection. the frequency of infectious com ... | 1988 | 3058911 |
| identification and localization of a novel, cytoskeletal, centrosome-associated protein in ptk2 cells. | antisera raised against centrin (salisbury, j.l., a.t. baron, b. surek, and m. melkonian. 1984. j. cell biol. 99:962-970) have been used, here, to identify a centrosome-associated protein with an mr of 165,000. immunocytochemistry indicates that this protein is a component of pericentriolar satellites, basal feet, and pericentriolar matrix of interphase cells. these components of pericentriolar material are, in part, composed of 3-8-nm-diam filaments, which interconnect to form a three-dimension ... | 1988 | 3060471 |
| [characteristics of the process of transferrin iron utilization by yersinia pestis at various incubation temperatures]. | the study has revealed that for the utilization of iron contained in transferrin the direct contact of y. pestis with this metalloprotein is necessary. at 28 degrees c y. pestis utilizes iron contained in transferrin. at 37 degrees c y. pestis absorbs transferrin, but cannot utilize its iron, which is probably linked with disturbances in the system of the transfer of iron from the transferrin receptor complex into the bacterial cell. | 1988 | 3061259 |
| antipeptide monoclonal antibodies inhibit the binding of rabies virus glycoprotein and alpha-bungarotoxin to the nicotinic acetylcholine receptor. | it has been reported that binding to muscle nicotinic acetylcholine receptor at the post-synaptic membrane is an important event of the rabies virus neurotropism. the binding site can be located within the 190-203 region of the virus glycoprotein sharing a high degree of homology with the "toxic loop" of the curare-mimetic snake neurotoxins. we have synthesized a tetradecapeptide corresponding to this glycoprotein region and used it, following conjugation with an immunogenic carrier to raise mab ... | 1988 | 3062388 |
| [the results of using an immunoenzyme method in an epizootiologic survey of natural siberian foci of plague]. | the approbation of the enzyme immunoassay in the study of diverse field material (corpses of animals, ectoparasites, soil, feces of birds of prey, etc.) for the presence of yersinia pestis contamination has shown the advantages of this assay over the existing serological tests: the passive hemagglutination test and the antigen (or antibody) neutralization test. the use of the immunoperoxidase preparation obtained on the basis of staphylococcal protein a makes it possible to detect antibodies to ... | 1988 | 3064503 |
| genetic analysis of human malignancy using somatic cell hybrids and monochromosome transfer. | evidence is presented for the existence of genes which regulate the malignant behaviour of human cancer cells. observations obtained from somatic cell hybrid and monochromosome transfer studies suggest that recessive genes play an important part in the control of malignant expression. loss of restriction fragment length polymorphism alleles, consistent with loss of these putative tumour-suppressor genes, is associated with an increasing number of human cancers. | 1988 | 3066478 |
| quality analysis of dsa equipment. | in the framework of a quality analysis project for the improvement of digital subtraction angiography (dsa) equipment, an inventory was made of the image quality and radiation dose of dsa equipments in six hospitals in the netherlands. the image quality was investigated with a contrast detail (cd) phantom. the entrance dose of the radiation on this phantom and the skin dose at the level of the eye lenses and the thyroid gland were measured in these hospitals using a human phantom during a standa ... | 1988 | 3067118 |
| quality analysis of dsa equipment. | in the framework of a quality analysis project for the improvement of digital subtraction angiography (dsa) equipment, an inventory was made of the image quality and radiation dose of dsa equipments in six hospitals in the netherlands. the image quality was investigated with a contrast detail (cd) phantom. the entrance dose of the radiation on this phantom and the skin dose at the level of the eye lenses and the thyroid gland were measured in these hospitals using a human phantom during a standa ... | 1988 | 3067118 |
| inhibition of the intraerythrocytic development of plasmodium falciparum in glucose-6-phosphate dehydrogenase deficient erythrocytes is enhanced by oxidants and by crisis form factor. | in this study, we have examined p. falciparum development in untreated normal or g6pd deficient erythrocytes as well as in parasitized cells exposed to isouramil--a fava bean extract known to induce oxidant stress in g6pd deficient erythrocytes (a condition known as favism), to diamide--a thiol oxidizing agent and to crisis form factor (cff)--a non-immunoglobulin product found in the sera of man immune to malaria. we observed a significant retardation in intraerythrocytic development of parasite ... | 1988 | 3067318 |
| ureaplasma urealyticum. overview with emphasis on fetal and maternal infections. | | 1988 | 3067640 |
| preparation of yersinia pestis plague murine toxin. | | 1988 | 3068484 |
| histochemical, immunofluorescence, and ultrastructural differences in fetal cartilage among three genetically distinct chondrodystrophic mice. | the severe lethal chondrodystrophies in man result in a common clinical syndrome including shortening of the face, mandible, and limbs. studies of three lethal chondrodystrophic mutants in mice, viz., chondrodysplasia (cho), cartilage matrix deficiency (cmd), and disproportionate micromelia (dmm), which share this syndrome, were performed with the aim of identifying histochemical, immunofluorescence, or ultrastructural differences which might exist among these hereditary cartilage disorders. we ... | 1988 | 3070812 |
| histochemical, immunofluorescence, and ultrastructural differences in fetal cartilage among three genetically distinct chondrodystrophic mice. | the severe lethal chondrodystrophies in man result in a common clinical syndrome including shortening of the face, mandible, and limbs. studies of three lethal chondrodystrophic mutants in mice, viz., chondrodysplasia (cho), cartilage matrix deficiency (cmd), and disproportionate micromelia (dmm), which share this syndrome, were performed with the aim of identifying histochemical, immunofluorescence, or ultrastructural differences which might exist among these hereditary cartilage disorders. we ... | 1988 | 3070812 |
| occurrence of unusual manifestations and uncommon serotypes of salmonella in human infection. | | 1988 | 3071548 |
| occurrence of unusual manifestations and uncommon serotypes of salmonella in human infection. | | 1988 | 3071548 |
| [immunoenzyme analysis using paper disks]. | filter paper discs have been used in the enzyme immunoassay (eia) as solid phase instead of polystyrene plates. the use of paper discs has made it possible to achieve a multiple increase in the sensitivity of sandwich eia, thus permitting the detection of yersinia pestis capsular antigen at a concentration of 0.4 ng/ml. paper discs can be used not only for the sorption of antigen and antibodies, but also for the affinity purification of preparations. | 1988 | 3072810 |
| extracellular matrix of the human retinal pigment epithelial cells in vitro. | | 1988 | 3073234 |
| localization of the kras2 oncogene in the domestic rabbit (oryctolagus cuniculus). | the kras2 gene was localized in the rabbit by chromosomal in situ hybridization, using a 3h-labeled human cdna probe. there were 201 silver grains on 144 metaphase spreads; 12.9% of the grains resided on chromosome 16, and 54% of these grains were located close to the centromere at 16p11----q11. statistical analysis indicated that labeling at this region represents a significant deviation from a random distribution and thus provided evidence for the assignment of kras2 to 16p11----q11. in additi ... | 1988 | 3073912 |
| [effect of the cultivation temperature on the physicochemical properties of the dna-dependent rna-polymerase of yersinia pestis]. | | 1988 | 3074242 |
| food intake of infant rats after intraperitoneal administration of insulin. | the effects of intraperitoneal insulin on the food intake have been determined in infant rats up to weaning. it was found that intraperitoneal (ip) insulin reduced the milk intake of 13 and 17 day-old pups for three hours after treatment. in 5, 9 and 24 day-old pups the food intake was not significantly changed after ip insulin administration. only in 28 day-old rat pups ip insulin induced an increase of food intake. since subcutaneously (sc) administered insulin gave rise to short-term hyperpha ... | 1988 | 3075555 |
| food intake of infant rats after intraperitoneal administration of insulin. | the effects of intraperitoneal insulin on the food intake have been determined in infant rats up to weaning. it was found that intraperitoneal (ip) insulin reduced the milk intake of 13 and 17 day-old pups for three hours after treatment. in 5, 9 and 24 day-old pups the food intake was not significantly changed after ip insulin administration. only in 28 day-old rat pups ip insulin induced an increase of food intake. since subcutaneously (sc) administered insulin gave rise to short-term hyperpha ... | 1988 | 3075555 |
| estrogenic control of spermidine/spermine n1-acetyltransferase activity in rat uterus. | | 1988 | 3076330 |
| plasmid-mediated virulence in yersiniae. | | 1987 | 3078833 |
| an nmr blood test for cancer: a critical assessment. | an evaluation of the reproducibility and accuracy of the nmr human blood test for cancer described by fossel, e. t., carr, j. m. and mcdonagh, j., (new england journal of medicine 315, 1369-1376) in 1986 has been conducted jointly at the national cancer institute-frederick cancer research facility, frederick, md (nci-fcrf) and the national research council, ottawa, canada (nrc). the influences on the test of the following were studied: (a) subject fasting; (b) sample collection, storage and hand ... | 1988 | 3079310 |
| an nmr blood test for cancer: a critical assessment. | an evaluation of the reproducibility and accuracy of the nmr human blood test for cancer described by fossel, e. t., carr, j. m. and mcdonagh, j., (new england journal of medicine 315, 1369-1376) in 1986 has been conducted jointly at the national cancer institute-frederick cancer research facility, frederick, md (nci-fcrf) and the national research council, ottawa, canada (nrc). the influences on the test of the following were studied: (a) subject fasting; (b) sample collection, storage and hand ... | 1988 | 3079310 |
| comparative study of encainide and quinidine in the treatment of ventricular arrhythmias. | the antiarrhythmic efficacy and safety of oral encainide hydrochloride and quinidine sulfate were compared in a nine center double-blind crossover study in 187 outpatients with benign or potentially lethal ventricular arrhythmias. patients with at least 30 premature ventricular complexes/h were randomized to receive either encainide, 25 mg four times/day, or quinidine, 200 mg four times/day, for 2 weeks. these doses were continued for another 2 weeks if a 75% or greater reduction in premature ve ... | 1986 | 3079781 |
| effect of dietary ascorbic acid and vitamin e on metabolic changes in rats and guinea pigs exposed to pcb. | the effect of the addition of dietary ascorbic acid and/or vitamin e (all-rac-alpha-tocopheryl acetate) in rats and guinea pigs exposed to pcb (polychlorinated biphenyls) was studied. rats were fed diets containing one of three levels of vitamin e (30, 500 or 1000 mg/kg diet) with or without pcb (200 mg/kg diet). guinea pigs were fed diets containing pcb (40 mg/kg diet) with 200 or 1000 mg ascorbic acid/kg diet and/or 70 or 2000 mg vitamin e/kg diet. for rats fed pcb, ascorbic acid in urine was ... | 1986 | 3080560 |
| second-generation cephalosporins. | | 1986 | 3081544 |
| mechanisms of h1o accumulation in mouse neuroblastoma cells differ with different treatments. | the accumulation of histone h1o in mouse nie-115 neuroblastoma cells was measured during the course of three treatments that block cell division. over the course of 12 days, these treatments, 5 mm butyrate, 2% dimethyl sulfoxide, and serum withdrawal, all resulted in decreased levels of dna synthesis and increased levels of h1o (in absolute terms and relative to the other h1 histones, h1abc). however, the increase in h1o differed comparing butyrate treatment, where there was a 6-fold increase in ... | 1986 | 3082878 |
| mechanisms of h1o accumulation in mouse neuroblastoma cells differ with different treatments. | the accumulation of histone h1o in mouse nie-115 neuroblastoma cells was measured during the course of three treatments that block cell division. over the course of 12 days, these treatments, 5 mm butyrate, 2% dimethyl sulfoxide, and serum withdrawal, all resulted in decreased levels of dna synthesis and increased levels of h1o (in absolute terms and relative to the other h1 histones, h1abc). however, the increase in h1o differed comparing butyrate treatment, where there was a 6-fold increase in ... | 1986 | 3082878 |
| ultrastructure of altered rat enamel beneath fluoride-induced cysts. | the effect of a single injection of sodium fluoride (60 mg/kg) on the development of rat molar enamel beneath fluoride-induced subameloblastic cysts was studied by transmission electron microscopy using undecalcified sections. three bands of altered enamel were identified and defined as the cyst surface band, the hypoplastic band, and the hypercalcified band. the irregular cyst surface band, not previously described, was found to have two components: electron-dense enamel globules and organic sp ... | 1986 | 3084740 |
| comparison of three actin-coding sequences in the mouse; evolutionary relationships between the actin genes of warm-blooded vertebrates. | we have determined the sequences of three recombinant cdnas complementary to different mouse actin mrnas that contain more than 90% of the coding sequences and complete or partial 3' untranslated regions (3'utrs): pam 91, complementary to the actin mrna expressed in adult skeletal muscle (alpha sk actin); paf 81, complementary to an actin mrna that is accumulated in fetal skeletal muscle and is the major transcript in adult cardiac muscle (alpha c actin); and pal 41, identified as complementary ... | 1986 | 3084797 |
| primary structure of the paramecium tetraurelia small-subunit rrna coding region: phylogenetic relationships within the ciliophora. | we have sequenced the coding region for the small-subunit rrna gene from paramecium tetraurelia. similarity comparisons between small-subunit rrnas from representatives of the metazoa, the plantae, the fungi and four other members of the ciliophora were used to construct phylogenetic trees. in these phylogenies the ciliophora diverged from the eukaryotic line of descent as a loose phylogenetic grouping during a radiative period that gave rise to the fungi, the plantae and the metazoa. | 1986 | 3084799 |
| some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate. | lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ... | 1986 | 3085110 |
| some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate. | lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ... | 1986 | 3085110 |
| immunochemical evidences that hexachlorobenzene induces two forms of cytochrome p-450 in the rat liver microsomes. | induction by hexachlorobenzene (hcb) of the liver microsomal system of metabolism of xenobiotics has been studied in comparison with the inductions by phenobarbital (pb) and 3-methylcholanthrene (mc). it has been shown that hcb increases the content of cytochrome p-450 in the microsomes. like pb, hcb induces the activities of aminopyrine- and benzphetamine-n-demethylases. at the same time hcb increases also the activities of benzpyrenehydroxylase and 7-ethoxyresorufin-o-deethylase, which are cha ... | 1986 | 3085962 |
| endogenous luteinizing hormone surge in women during induction of multiple follicular development with pulsatile follicle stimulating hormone. | in this study nine consecutive normally cycling women undergoing in-vitro fertilization (ivf) were superovulated with clomiphene citrate followed by pulsatile 'pure' fsh injected s.c. via a pump (28 iu every 3 h). all women displayed an endogenous lh surge, which was markedly attenuated in most of the cases (peak value 44.5 +/- 5.9 u/l, duration 29.2 +/- 1.2 h, mean +/- sem) as compared to spontaneous cycles. an increase in serum progesterone levels before the onset of the lh surge was seen in o ... | 1986 | 3085995 |
| survey of aminoglycoside resistance patterns. | we studied the susceptibility of 545 gram-negative clinical isolates to six aminoglycosides: gentamicin, tobramycin, netilmicin, amikacin, 2'-n-ethylnetilmicin, and 6'-n-ethylnetilmicin. the resistant strains (359) have been classified into aminoglycoside resistance (patterns) phenotypes (agrps) corresponding to known resistance mechanisms. the results clearly demonstrated that most strains of proteus spp., klebsiella spp., escherichia coli and pseudomonas aeruginosa appeared to be able to adeny ... | 1986 | 3087636 |
| gastric form of alpha chain disease. | a case of alpha chain disease, involving the stomach only, is reported in an algerian man suffering from epigastric pains. upper digestive tract fibreoptic endoscopy showed two antral ulcers and an ulcerative gastritis pattern, which promptly disappeared with cimetidine treatment. antral biopsies at a distance from the ulcers, but not of the ulcer crater itself, disclosed a dense infiltration of antral lamina propria by mature or sometimes atypical plasma cells. on transmural surgical antral bio ... | 1986 | 3087826 |
| antibodies to la, jo-1, nrnp and sm detected by multi-track immunoblotting using a novel filter holder: a comparative study with counterimmunoelectrophoresis and immunodiffusion using sera from patients with systemic lupus erythematosus and sjögren's syndrome. | the use of western blotting or immunoblotting to detect autoantibodies in the serum of patients with autoimmune connective tissue diseases was investigated. an apparatus suitable for simultaneously screening 16 sera on immunoblots was used to show that a complex pattern of antibody binding polypeptides was present in whole hela cells. a simpler and readily interpreted pattern of binding was achieved using affinity-purified rabbit thymus antigens. seventy-seven patients with systemic lupus erythe ... | 1986 | 3088125 |
| molecular biology of homo sapiens. | | 1986 | 3088726 |
| characterization of cyanobacterial ferredoxin-nadp+ oxidoreductase molecular heterogeneity using chromatofocusing. | chromatofocusing has been used as an analytical tool to check preparations of the enzyme ferredoxin-nadp+ oxidoreductase (ec 1.18.1.2) purified in either the presence or absence of the serine protease inhibitor phenylmethylsulfonyl fluoride from the cyanobacterium anabaena sp. strain 7119. only one isoelectric species was found when the crude extract was processed in the presence of the protease inhibitor. nevertheless, when the inhibitor was omitted, four ionic forms of the enzyme--showing appa ... | 1986 | 3089056 |
| standardization and evaluation of the clinical usefulness of mycobacterial skin test antigens. | | 1986 | 3090420 |
| effects of the binding of myosin light chain kinase on the reactivities of calmodulin lysines. | the effects of the binding of smooth muscle myosin light chain (mlc) kinase on the microenvironments of different regions of calmodulin (cam) were investigated by comparing the acylation rate constants of the seven lysine amino groups of free cam with those of cam complexed with mlc kinase. equimolar amounts of cam and cam-mlc kinase complex were trace labeled with [3h]acetic anhydride in the presence of phenylalanine as a standard nucleophile. after completion of the reaction, equal amounts of ... | 1986 | 3091599 |
| galactosyltransferase purified from rat milk is distinct from the human and bovine enzyme. | n-acetylglucosaminide beta 1, 4-galactosyltransferase (ec 2.4.1.22) was purified from rat milk by affinity chromatography on n-acetylglucosamine-sepharose and alpha-lactalbumin-sepharose columns. the purified enzyme migrated as three polypeptides of relative molecular weight 59,000, 54,000, and 27,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. antiserum raised against the 54k rat protein immunoprecipitated all three polypeptides, suggesting that they share common antigenic sit ... | 1986 | 3091666 |
| calspectin (fodrin or nonerythroid spectrin)-actin interaction: a possible involvement of 4.1-related protein. | the calspectin/actin complex extracted from the bovine brain membrane crosslinks f-actin, resulting in the increasing viscosity of f-actin determined by low-shear viscometry. we demonstrated the presence of a protein factor in this complex, which regulated the calspectin-f-actin interaction in a ca2+- and calmodulin-dependent manner. erythrocyte protein 4.1, but not synapsin i, mimics the function of this brain factor using a reconstitution system including purified calspectin, calmodulin and f- ... | 1986 | 3096326 |
| comparative tests for detection of plague antigen and antibody in experimentally infected wild rodents. | the enzyme-linked immunosorbent assay (elisa) was compared with other standard tests for detection of plague (yersinia pestis) antibody and antigen in multimammate mice (mastomys coucha and m. natalensis) which were experimentally infected and then killed at daily intervals postinoculation. for detection of antibody in sera from m. natalensis, the immunoglobulin g (igg) elisa was equivalent in sensitivity to passive hemagglutination and more sensitive than the igm elisa and complement fixation. ... | 1986 | 3097065 |
| [prevalence of campylobacter jejuni complement fixing antibody in healthy people]. | | 1986 | 3097197 |
| [combined efficacy of polyamine antimetabolites and cis-diamminedichloroplatinum]. | the combined antitumor effects of the polyamine antimetabolites, alpha-difluoro methylornithine (dfmo) and methylglyoxal-bis-guanylhydrazone (mgbg), with cddp were studied using human gastric cancer cells xenotransplanted into nude mice. dfmo (1000 mg/kg in two divided doses) and mgbg (50 mg/kg) were given ip for six consecutive days from the time when the xenotransplanted tumor weighted about 100 mg, and cddp (3.0 mg/kg) was given ip every other day from the same time. animals treated with dfmo ... | 1986 | 3100839 |
| galactosyltransferase: multiple forms in serum of normal and hepatoma mc-29 bearing chickens and from liver and hepatoma microsomal and plasma membrane preparations. | the multiple forms of galactosyltransferase in chicken serum and in microsomal and plasma membrane preparations from liver and viral induced hepatoma mc-29 have been studied by isoelectric focussing. an elevation of the hepatoma plasma membrane enzyme activity was described and in the pattern of the multiple forms of the enzyme two forms were found (pi-5.34 and 8.22) which were similar to those described in the serum of hepatoma bearing chickens (pi-5.36 and 8.24). a conclusion is drawn that the ... | 1986 | 3102037 |
| [effect of bacterial toxins on microcirculation (experimental research)]. | histological and biomicroscopic methods were used to study the effects of toxic lemic and meningococcal microbial fractions on mesenteric blood microcirculation in rats. aggregation ability of red blood cells and thrombocytes was investigated. the sublethal dose of meningococcal toxins was shown to induce marked impairment in the blood microcirculation 30 minutes after intravenous administration. the lethal dose of lemic toxins manifests itself later (in 2-4 hours). meningococcal toxins are char ... | 1987 | 3105520 |
| [transport of basic amino acids into yersinia pestis cells]. | the transport of the basic amino-acids into the plague microbe cells has been shown to proceed by means of two transfer systems, the common transfer system and the system specific for lysine. besides that, arginine can be transported into the plague microbe cells by the histidine transport system. the vaccine strain ev niieg was used as a model to study the amino-acid transport systems. | 1987 | 3106795 |
| effects of neonatal estrogenization on rat bone development: a histomorphometric study. | the effects of a single dose of 500 micrograms of estradiol benzoate, administered on the first day of life, on rat bone development have been histomorphometrically studied at 15 days of age. estrogenized animals presented decreased total tibial length (16.55 +/- 0.50 vs. 17.84 +/- 0.73 mm, p less than 0.05) and increased thickness of the cartilage growth plate (528.92 +/- 13.30 vs. 382.77 +/- 37.85 micron, p less than 0.01). this increase was mostly due to the presence of a wider (p less than 0 ... | 1987 | 3107771 |
| reflex regulation of atrioventricular conduction. | we evaluated the time course of baroreflex modulation of atrioventricular (av) nodal conduction in anesthetized dogs (n = 28). beat-by-beat changes in heart rate (hr) and av interval (avi) evoked by transient alterations in arterial pressure (ap) were recorded in the intact state, after vagotomy, and following stellectomy. under each experimental condition, alterations in ap induced parallel changes in hr and avi with maximum hr and avi responses occurring simultaneously. in three animals, ap al ... | 1987 | 3109256 |
| effect of blood pressure on medial medulla-induced muscle atonia. | stimulation of the medial medullary reticular formation (mmrf) has long been reported to produce generalized inhibition of skeletal muscle activity. however, several studies have reported that in most cases mmrf stimulation produces only increases in muscle tone. in the present investigation we have found that blood pressure is a critical variable, determining whether mmrf stimulation will produce muscle excitation or inhibition. when mean arterial pressure (map) was greater than 80 mmhg but les ... | 1987 | 3109262 |
| differences between saccharomyces cerevisiae and bacillus subtilis in secretion of human lysozyme. | saccharomyces cerevisiae secreted human lysozyme in the medium as an active form when the signal peptides of chicken lysozyme and a chicken lysozyme-aspergillus awamori glucoamylase hybrid were used, whereas it did not synthesize any human lysozyme protein by using the signal peptide of a. awamori glucoamylase. the secreted lysozyme was easily purified and crystallized. on the other hand, bacillus subtilis secreted an inactive human lysozyme, which seemed to have incorrect disulfide bonds, with ... | 1987 | 3109419 |
| differences between saccharomyces cerevisiae and bacillus subtilis in secretion of human lysozyme. | saccharomyces cerevisiae secreted human lysozyme in the medium as an active form when the signal peptides of chicken lysozyme and a chicken lysozyme-aspergillus awamori glucoamylase hybrid were used, whereas it did not synthesize any human lysozyme protein by using the signal peptide of a. awamori glucoamylase. the secreted lysozyme was easily purified and crystallized. on the other hand, bacillus subtilis secreted an inactive human lysozyme, which seemed to have incorrect disulfide bonds, with ... | 1987 | 3109419 |
| the effect of sodium valproate on the subcellular particles of the rat liver. | sodium valproate, 200 mg/kg, i.p., for three weeks produced an increase in the mean sedimentation coefficient of liver mitochondria, while no changes were observed in lysosomes and peroxisomes. as the mean density of the mitochondria, the lysosomes and the peroxisomes of the control and treated animals were not different, it was concluded that the size of the mitochondria had increased in the liver of the treated rats. the mean sedimentation coefficient of the mitochondria returned to normal aft ... | 1987 | 3109433 |
| a potential mechanism of dl-beta-hydroxybutyrate-induced malformations in mouse embryos. | dl-beta-hydroxybutyrate (dl-bohb) is teratogenic to rodent embryos in culture, but the biochemical mechanism(s) responsible for the induction of malformations has not been elucidated. thus, to delineate a potential mechanism, the interaction of the ketone body with embryonic glucose metabolism was investigated. mouse embryos were exposed in whole embryo culture to dl-bohb or each isomer separately during the period of neurulation. the results demonstrate that dl-bohb inhibits glucose oxidation b ... | 1987 | 3111272 |
| implication of ornithine decarboxylase and polyamines in pancreatic growth of neonatal rats. | the current study examines the effects of alpha-difluoromethylornithine (dfmo), an irreversible ornithine decarboxylase inhibitor, on pancreatic growth and development of rat neonates. newborn rats were given daily subcutaneous injections of 300 or 500 mg kg-1 dfmo and killed after 7, 14, 21, and 28 days of treatment. pancreatic weights and dna, rna, protein, amylase and chymotrypsin total contents (per pancreas) and concentrations were evaluated at the end of each period. inhibition of body wei ... | 1987 | 3114741 |
| [the structure of the variable gene for the kappa chains of antibodies produced by hybridoma ptf-02]. | the genome of hybridoma ptf-02 has two genes for the kappa chains, and only one of these codes for the synthesis of the antibody light chains. the nucleotide sequences corresponding to the leader peptide and to the variable region of this gene were determined. an amino acid sequence corresponding to exons has been proposed on the basis of the nucleotide sequence. a nucleotide sequence adjacent to the gene at the 5'-end has also been determined, in particular, the precise localization of tata- an ... | 1987 | 3116399 |
| parenteral antischistosomal therapy: a potential risk factor for hepatitis b infection. | to study the association between hepatitis b and schistosomiasis, 1,234 egyptian males, ages 18 to 24, were interviewed, examined, and tested for schistosoma mansoni infection and hbsag. sera from 91 (7.4%) of the study subjects were positive for hbsag, and s. mansoni was found in the stools of 26.3%. there was no correlation between s. mansoni infection, with or without hepatosplenomegaly, or a history of schistosomiasis, and hbsag. an association was found between hbsag positivity and a previo ... | 1987 | 3119770 |
| doctors against nuclear war in turkey. | | 1987 | 3120914 |
| evaluation of the 11co2 positron emission tomographic method for measuring brain ph. i. ph changes measured in states of altered pco2. | the 11co2 method for measuring local brain ph with positron emission tomography (pet) has been experimentally evaluated, testing the adequacy of the kinetic model and the ability of the method to measure changes in brain ph. plasma and tissue time/activity curves measured during and following continuous inhalation of 11co2 were fit with a kinetic model that includes effects of tissue ph, blood flow, and fixation of co2 into compounds other than dissolved gas and bicarbonate ions. for each of ten ... | 1987 | 3121647 |
| [solid phase radioimmunoassay for the quantitative determination of antigen-specific igg-antibodies in the serum of patients with farmer's lung]. | in this study we have investigated a number of technical aspects of a sensitive solid-phase radioimmunoassay (microtitre-plate ria) for the quantitative determination of antigen-specific igg antibodies in the sera of patients suffering from farmer's lung. microtitre-plates were coated with antigens of aspergillus fumigatus, micropolyspora faeni, aureobasidium pullulans, alternaria tenuis, thermoactinomyces vulgaris and hay. optimal antigen concentration and serum dilution were determined, the te ... | 1987 | 3121779 |
| stereoselective fungal metabolism of 7,12-dimethylbenz[a]anthracene: identification and enantiomeric resolution of a k-region dihydrodiol. | syncephalastrum racemosum ut-70 and cunninghamella elegans atcc 36112 metabolized 7,12-dimethylbenz[a]anthracene (7,12-dmba) to hydroxymethyl metabolites as well as 7-hydroxymethyl-12-methylbenz[a]anthracene trans-3,4-, -5,6-, -8,9-, and -10,11-dihydrodiols. the 7,12-dmba metabolites were isolated by reversed-phase high-performance liquid chromatography and identified by their uv-visible absorption, mass, and nuclear magnetic resonance spectral characteristics. a comparison of the circular dichr ... | 1987 | 3122656 |
| rescue of menotrophin cycles prone to develop ovarian hyperstimulation. | in an attempt to prevent the loss of 'overstimulated cycles' associated with human menopausal gonadotrophin (hmg)-induced ovulation, oestradiol levels and ovarian follicular state were monitored in 12 women with 'overstimulated cycles' after withholding hmg for several days. human chorionic gonadotrophin (hcg) was administered when oestradiol levels were less than or equal to 1700 pg/ml and the leading follicles between 17 and 22 mm in diameter. during the withholding period follicular growth co ... | 1987 | 3122819 |
| release of opioid peptides from the spinal cord of rats subjected to chronic pain. | chronic localized pain increased the level of the opioid peptides, dynorphin (dyn), alpha-neoendorphin (aneo), met-enkephalin (met) and mets-enkephalin-arg6-gly7-leu8 (meagl), in the lumbar enlargement of the rat spinal cord. it was accompanied with a reduction of the spontaneous and k+-stimulated release of aneo and meagl from spinal cord slices in vitro and a decreased release of aneo from the spinal cord in vivo. the results indicate that the reduction in the activity of endogenous opioid pep ... | 1986 | 3123959 |
| ethical aspects of military physicians treating patients with hiv/part two: the duty to take initiative. | | 1988 | 3126439 |
| [studies on the antitumor effects of human interferon (2). effects of massive pulsatile administration of rifn-gamma on advanced renal cell carcinoma]. | | 1987 | 3127620 |
| human antibody responses against schistosomal antigens. i. antibodies from patients with ancylostoma, ascaris lumbricoides or schistosoma mansoni infections react with schistosome antigens. | sera of patients with either hookworm infections or ascariasis, who are from areas nonendemic for schistosoma mansoni, contain antibody reactivity against extracts of s. mansoni schistosomula. such patients with hookworm, but not those with ascaris lumbricoides, also express considerable antibody activity against intact schistosomula and schistosomular membrane preparations. sera from patients with schistosomiasis mansoni also contain these activities. because these three helminthic infections o ... | 1988 | 3128128 |
| the identification and antimicrobial susceptibility testing of neisseria gonorrhoeae, 1980-1987. results from the college of american pathologists microbiology surveys program. | the results of the college of american pathologists (skokie, iii) microbiology surveys from 1980 through 1987 indicate subscriber laboratories have continued to improve their ability to identify and test the antimicrobial susceptibility of neisseria gonorrhoeae. bacteriology and comprehensive surveys participants correctly identified this organism more frequently (95% to 96%) than did the basic surveys laboratories. neisseria gonorrhoeae samples in mixed cultures were slightly more difficult (3% ... | 1988 | 3128961 |
| evidence for thromboxane biosynthesis in established cell lines derived from human lung adenocarcinomas. | thromboxane b2 (txb2) is the stable nonenzymatic hydrolysis product of thromboxane a2, a substance implicated in the initiation of the facilitative role of thrombocytes in the metastatic process. txb2 was isolated from protein-free culture medium of cell lines calu-3, calu-6, a549, and a549/asc-1, derived from human lung adenocarcinomas. txb2 and other 20-carbon fatty acid cyclooxygenase products synthesized from exogenous and endogenous arachidonic acid were identified by their characteristic r ... | 1988 | 3129183 |
| n-0437: a selective d-2 dopamine receptor agonist in in vitro and in vivo models. | the selectivity of the potent dopamine d-2 agonist 2-(n-propyl-n-2-thienylethylamino)-5-hydroxytetralin (n-0437) was examined in a series of in vivo and in vitro pharmacological models. in radioligand binding assays, n-0437 showed high potency (ki = 0.69 nm) and selectivity for d-2 receptors as compared to its potency and selectivity at various other neuronal receptors (ki in nm): d-1 (678) dopamine, alpha 1-(534) and alpha 2-(195) adrenoceptor, s1-(6940) and s2-(5900) serotonin and muscarine (2 ... | 1988 | 3130270 |
| the inhibition of pb125i formation in calf thyroid caused by 14-iodo-15-hydroxy-eicosatrienoic acid is due to decreased h2o2 availability. | previous work from our laboratory has shown that 14-iodo-15-hydroxy-5,8,11-eicosatrienoic acid (i-ho-a) is a potent inhibitor of iodine organification in calf thyroid slices. the present studies were performed in order to clarify the mechanism of this action. incubation of thyroid slices with 10(-4)m i-ho-a caused a 47 and 53% decrease in pb125i formation after 30 and 60 min incubation, respectively. in a series of experiments an inverse relationship between the degree of inhibition caused by i- ... | 1988 | 3131225 |
| j chain in rana catesbeiana high molecular weight ig. | a polypeptide homologous to human and mouse j chain has been identified in the high molecular weight (hmw) ig of the bullfrog, rana catesbeiana. in previous studies, we had detected a component that was similar in size to mammalian j chains and that, relative to l chains, migrated rapidly to the anode in alkaline-urea page; however, its mobility was less than that of mammalian j chains. we now demonstrate that this component is covalently linked to the h chain of r. catesbeiana hmw ig. all of th ... | 1988 | 3131430 |
| [effects of grf 1-29 in normal and hypotrophic lambs]. | injections of hgrf rapidly stimulated gh secretion in lamb and this response was significantly higher in hypotrophic than in normal lambs. the treatment had an effect on body composition reducing the retention of lipids and the energy level and increasing calcium and phosphorus content. the results showed that grf affected both metabolism and skeletal growth of ruminants, stimulating specially the production of sm-c and proliferation of chondrocytes. | 1988 | 3131856 |
| treatment of canine embolic pulmonary hypertension with recombinant tissue plasminogen activator. efficacy of dosing regimes. | we investigated effects of two dosing regimes of recombinant tissue plasminogen activator (rt-pa) and sodium heparin on pulmonary thrombolysis in a canine model of pulmonary hypertension, induced by injection of radioactive blood clots. by continuously counting over both lung fields with a mobile gamma camera, we correlated rate and extent of pulmonary thrombolysis with corresponding pulmonary hemodynamics. treatment with heparin, over a 3-hour interval, did not result in significant thrombolysi ... | 1988 | 3133136 |
| micronucleus assay in human fibroblasts: a measure of spontaneous chromosomal instability and mutagen hypersensitivity. | by comparing fibroblast strains derived from individuals exhibiting chromosome instability and/or mutagen hypersensitivity (cockayne syndrome, ataxia telangiectasia, and fanconi anemia) with strains derived from healthy donors, the fibroblast micronucleus assay has been established as a reproducible measure of the genotypic variation in spontaneous or mitomycin c (mmc)-induced chromosomal instability. the patient strains that were moderately or exquisitely sensitive to mmc, whereas the mildly se ... | 1988 | 3133207 |
| strain differences in n-butyl-n-(4-hydroxybutyl)nitrosamine bladder carcinogenesis in rats. | differences in susceptibility of the urinary bladder epithelium to n-butyl-n-(4-hydroxybutyl)-nitrosamine (bbn) in various strains were examined. in experiment 1, 5 strains of male rats were given 0.025% bbn in the drinking water for 8 weeks followed by drinking water without bbn for 32 weeks. analbuminemic rats (nar) and aci rats had high incidences of urinary bladder lesions (papillary or nodular hyperplasia, papilloma and carcinoma), f344 and wistar rats had low incidences, and sprague-dawley ... | 1988 | 3133335 |
| immunocytochemical delineation of thyrotrophic area: origin of thyrotropin-releasing hormone in the median eminence. | to identify the specific thyrotropin-releasing hormone (trh)-containing neurons projecting to the median eminence (me), a retrograde tracing method with horseradish peroxidase (hrp) was combined with immunocytochemical staining for trh. three days after hrp injection restricted to the me, several trh-positive neuronal perikarya were found to contain hrp. such double-stained cells were exclusively distributed in the anterior parts of the periventricular nucleus and the most medial parts of the pa ... | 1988 | 3135505 |
| [dermatoglyphic changes of a familial nature associated with an inversion of the pair 1 chromosomes]. | | 1988 | 3143141 |
| crystals in calcified epiphyseal cartilage and cortical bone of the rat. | the size of hydroxyapatite (hap) crystals in calcified cartilage and cortical bone of the rat has been studied and compared with that of synthetic poorly crystalline hydroxyapatite (pcha). crystal size was determined by x-ray diffraction and selected-area dark field imaging, and their elemental compositions were determined by emission spectroscopy. the crystal size of cartilage, bone, and pcha were found to be between 120 and 170 a in length by 50 a in width as determined by both x-ray diffracti ... | 1988 | 3145127 |
| interactions between angiotensin ii, sympathetic nerve-mediated pressor response and cyclo-oxygenase products in the pithed rat. | 1. the influence of angiotensin ii (aii) on resting blood pressure and on sympathetic nerve-mediated pressor responses in the pithed rat was investigated either by inhibiting the renin-angiotensin system or by infusing aii. 2. plasma aii levels in the pithed rat were approximately 20 fold higher than in normotensive rats. 3. infusion of a subpressor dose of aii (50 ng kg-1 min-1) had no effect on sympathetic nerve mediated pressor responses but a pressor dose of aii, (200 ng kg-1 min-1) facilita ... | 1988 | 3146400 |
| antischistosomal treatment and the measurement of incidence of schistosomiasis in a community of high transmission. | there is disagreement in the measurement of incidence in schistosomiasis concerning which of two categories of negatives is representative of the population of an endemic area: naturally uninfected individuals (untreated negatives) and those made negative following treatment (treated negatives). the incidence of schistosomiasis was studied in the two categories of negatives for two periods among 208 school children (6-11 years old) living in a community of high transmission in zimbabwe. in both ... | 1988 | 3147550 |
| periovulatory serum prolactin and progesteron secretion in gonadotropin stimulated cycles. | in the group of 18 normoprolactinemic patients stimulated by human menopausal gonadotropins (hmg), serum prolactin (prl) and progesteron (p) levels were evaluated at the time of estradiol (e2) increase before, during and after chorionic gonadotropin (hcg) administration. in 8 patients (group i) mean serum prl levels remained normal, ranging from the mean basal values (12.4 +/- 1.7 micrograms/l) to the mean values at the time of hcg administration (16.8 +/- 1.8 micrograms/l) and after it (24.9 +/ ... | 1988 | 3147564 |
| in vivo measurement of intracellular ph in human brain during different tensions of carbon dioxide in arterial blood. a 31p-nmr study. | the effect of changes in carbon dioxide tension in arterial blood upon intracellular ph in brain tissue was studied in seven healthy volunteers, aged 22-45 years. the ph changes were monitored by use of 31p nuclear magnetic resonance spectroscopy, performed on a whole-body 1.5 tesla siemens imaging system. the measurements were carried out during hyperventilation and with the subject breathing atmospheric air containing 5 vol. % and 7 vol. % carbon dioxide. intracellular ph increased significant ... | 1988 | 3147578 |