| [design of a species-specific dna probe based on the pfra plasmid of the plague pathogen]. | the recombinant plasmid pbs1 carrying a 2 kb salgi fragment of yersinia pestis pfra plasmid was constructed by insertion of the fragment into a vector plasmid pbr327. salgi-bspri 400 bp subfragment was recloned into a pbr322 vector plasmid. open reading frame was found in the fragment by dna sequencing technique. the subfragment designated f1-probe permits one to identify specifically the yersinia pestis strains harbouring pfra plasmid, thus, differing them from closely related yersiniea and oth ... | 1991 | 1758469 |
| spectrin breakdown products increase with age in telencephalon of mouse brain. | calcium activated proteolysis of brain spectrin produces characteristic breakdown products (bdps), the concentrations of which increase markedly in many instances of brain pathology. results reported here indicate that levels of the bdps rise with age (3-30 months) in the telencephalon but not in the hindbrain of balb/c mice. these observations suggest that spectrin breakdown is a pathologic biochemical marker which increases with age in some but not all brain regions. | 1991 | 1762696 |
| analysis of high psa n-cam expression during mammalian spinal cord and peripheral nervous system development. | using a monoclonal antibody that recognizes specifically a high polysialylated form of n-cam (high psa n-cam), the temporal and spatial expression of this molecule was studied in developing spinal cord and neural crest derivatives of mouse truncal region. temporal expression was analyzed on immunoblots of spinal cord and dorsal root ganglia (drgs) extracts microdissected at different developmental stages. analysis of the ratio of high psa n-cam to total n-cam indicated that sialylation and desia ... | 1991 | 1769342 |
| salmonella enterica subspecies diarizonae bacteremia in an infant with enteritis--a case report. | the septicemia caused by the arizona group organism is rare and usually observed in adults with underlying diseases. in korea, salmonella infection is common, but a report of arizona infection is unknown. we isolated s. entercia subsp. diarizonae from blood of a 6-month-old infant. the serovar was determined as 28:z10:-, a rare one in america. the isolate was susceptible to ampicillin, chloramphenicol, cotrimoxazole and others. the patient rapidly recovered with ampicillin and gentamicin therapy ... | 1991 | 1781188 |
| salmonella enterica subspecies diarizonae bacteremia in an infant with enteritis--a case report. | the septicemia caused by the arizona group organism is rare and usually observed in adults with underlying diseases. in korea, salmonella infection is common, but a report of arizona infection is unknown. we isolated s. entercia subsp. diarizonae from blood of a 6-month-old infant. the serovar was determined as 28:z10:-, a rare one in america. the isolate was susceptible to ampicillin, chloramphenicol, cotrimoxazole and others. the patient rapidly recovered with ampicillin and gentamicin therapy ... | 1991 | 1781188 |
| expression of normal and variant human hypoxanthine-guanine phosphoribosyltransferase in e. coli. | 1. ecpr is a rapid and effective means for generating recombinant human hprt. 2. the bl21 (de3) t7 polymerase/t7 promoter system provides high level expression of human hprt constructs after induction of the t7 polymerase gene with iptg. 3. human hprt constructs expressed in e. coli mimic the variant properties originally demonstrated in lymphoblast extracts from affected individuals. 4. human hprt expressed in e. coli can be rapidly purified to near homogeneity by a two step purification scheme ... | 1991 | 1781351 |
| [protein expression of yersinia pestis during changes due to long-term cultivation in vivo]. | the electrophoretic study of yersinia pestis proteins made possible to find the significant modification of yersinia pestis polypeptide specters when the bacteria were cultivated in semi-penetrable cells implanted into the guinea pigs peritoneum. the proteinogramms of the isolates from the implanted cells lacked the stained bands characteristic of yersinia pestis cells grown in vitro and contained the new polypeptides absent from the bacteria grown on the hottinger agar plates. the difference wa ... | 1991 | 1784300 |
| [cloning and detailed mapping of the fra-ymt region of the yersinia pestis pfra plasmid]. | the genetical libraries of the pfra plasmid of yersinia pestis genes were obtained by insertion into the psti, salgi, ecori, xhoi restriction sites of the cosmid vector phc79. the immunochemical analysis of the recombinant clones has revealed the clones synthesizing the antigen fl (fraction i) and mouse toxin (ymt--yersinia pestis murine toxin). the restriction analysis of the plasmids from antigen synthesizing clones has permitted to construct the detailed physical map of the fra-ymt region of ... | 1991 | 1787840 |
| [the gene identification of bacterial species and serovariants by the polymerase chain reaction with universal oligonucleotides: the reidentification of earlier isolated strains of yersinia pseudotuberculosis]. | genetic analysis of 19 standard strains belonging to 6 yersinia species (y. pestis, y. pseudotuberculosis, y. enterocolitica, y. kirstensenii, y. frederiksenii, y. intermedia) revealed that gene typing by the method of polymerase chain reaction (pcr) with the use of universal primers permitted the identification of species in bacterial cultures by pcr patterns and the determination of y. pseudotuberculosis serovars within 4 hours. by this method 23 y. pseudotuberculosis strains (serovar 1), earl ... | 1991 | 1789029 |
| real-time monitoring of slow-wave sleep by electroencephalogram variance. | based on statistical variance as an index of electroencephalogram (eeg) parameters, we monitored slow-wave sleep in both humans and rats in real time and on-line with a widely used personal computer. this eeg variance method may be a useful tool to carry out biological rhythm research, including sleep studies. | 1991 | 1794153 |
| aspirin-like drugs, ethanol-induced rat gastric injury and mucosal eicosanoid release. | the effect of oral administration of various non-steroidal antiinflammatory drugs on ethanol-induced rat gastric injury and mucosal release of leukotriene c4, 6-keto-prostaglandin f1 alpha and 15-hydroxy-5,8,11,13-eicosatetraenoic acid was investigated. it was found that besides sodium salicylate and high doses of aspirin, other salicylate-type drugs, such as diflunisal, 4-aminosalicylic acid, 2,4-dihydroxybenzoic acid and methyl salicylate, and several non-acidic compounds, such as proquazone, ... | 1991 | 1794414 |
| catalase activity in cultured human retinal pigment epithelial cells. | | 1991 | 1794560 |
| structural and biochemical changes in the sclera of experimentally myopic eyes. | | 1991 | 1794573 |
| air pollution and asthma: clinical studies with sulfuric acid aerosols. | until recently, acid deposition has been widely considered a serious ecological problem but not a threat to human health. the controlled clinical study is an important approach in linking acidic aerosol inhalation with respiratory effects. asthmatic patients represent a subpopulation most responsive to sulfuric acid aerosols. in a series of studies with asthmatic volunteers, several factors have been identified that may modulate the intensity of the bronchoconstrictor response to inhaled acidic ... | 1991 | 1794691 |
| structural characterization of the urinary metabolites of iprindole in rat. | 1. the in vivo metabolism of iprindole in rat is described. each rat received a single dose of iprindole (10 mg/kg) and urine was collected for 48 h. 2. fourteen metabolites of iprindole were isolated from rat urine after enzymic hydrolysis and their structures were determined by a computerized g.l.c.-mass spectrometric technique, before and after appropriate chemical derivatization. 3. three concurrent metabolic pathways have been identified for iprindole in rat; aromatic ring hydroxylation is ... | 1991 | 1796616 |
| urinary metabolites of doxapram in premature neonates. | 1. urine samples from 20 premature neonates who received doxapram by i.v. infusion were analysed for drug metabolites by g.l.c-mass spectrometry. 2. in addition to doxapram, all urines contained at least one metabolite, but the known metabolite, 3-ketodoxapram, was detected in only 50% of the samples, and in some instances only in trace amounts. 3. significant inter-individual differences in the metabolic pathways of doxapram were observed. 4. a total of six metabolites of doxapram were isolated ... | 1991 | 1796617 |
| local intracoronary infusions of bradykinin profoundly reduce the severity of ischaemia-induced arrhythmias in anaesthetized dogs. | bradykinin in a dose (25 ng kg-1 min-1) which did not alter coronary flow, or saline, were infused into a small branch of the left anterior descending coronary artery in dogs anaesthetized with chloralose and urethane, for 10 min prior to coronary artery occlusion and throughout the 25 min occlusion period. the degree of inhomogeneity of conduction and epicardial st-segment changes were measured in the ischaemic zone with a composite electrode. in control dogs, coronary artery occlusion led to s ... | 1991 | 1797297 |
| local intracoronary infusions of bradykinin profoundly reduce the severity of ischaemia-induced arrhythmias in anaesthetized dogs. | bradykinin in a dose (25 ng kg-1 min-1) which did not alter coronary flow, or saline, were infused into a small branch of the left anterior descending coronary artery in dogs anaesthetized with chloralose and urethane, for 10 min prior to coronary artery occlusion and throughout the 25 min occlusion period. the degree of inhomogeneity of conduction and epicardial st-segment changes were measured in the ischaemic zone with a composite electrode. in control dogs, coronary artery occlusion led to s ... | 1991 | 1797297 |
| renovascular hypertension impairs formation of endothelium-derived relaxing factors and sensitivity to endothelin-1 in resistance arteries. | 1. endothelium-dependent vascular regulation was investigated in mesenteric resistance arteries of goldblatt two kidney-one clip (2k1c) renovascular hypertensive rats. 2. third order branches of mesenteric arteries were dissected free and mounted on glass cannulae in an organ chamber. changes in vascular diameter were measured in pressurized and perfused arteries with a video dimension analyzer. 3. acetylcholine evoked endothelium-dependent relaxations that were much more pronounced with intralu ... | 1991 | 1797302 |
| renovascular hypertension impairs formation of endothelium-derived relaxing factors and sensitivity to endothelin-1 in resistance arteries. | 1. endothelium-dependent vascular regulation was investigated in mesenteric resistance arteries of goldblatt two kidney-one clip (2k1c) renovascular hypertensive rats. 2. third order branches of mesenteric arteries were dissected free and mounted on glass cannulae in an organ chamber. changes in vascular diameter were measured in pressurized and perfused arteries with a video dimension analyzer. 3. acetylcholine evoked endothelium-dependent relaxations that were much more pronounced with intralu ... | 1991 | 1797302 |
| structural investigations of glucans from cultures of glomerella cingulata spaulding & von schrenck. | methylation analysis, enzymic digestion, n.m.r. spectroscopy, and smith degradation showed that the major extracellular polysaccharide, isolated from cultures of the fungus glomerella cingulata, was a (1----3)-beta-d-glucan with side chains of 1-4 (1----3)-linked beta-d-glucose residues attached to position 6. a (1----6)-beta-d-glucan was produced by the fungus in small proportions. treatment of the (1----3,1----6)-beta-d-glucan (890,315) with greater than 0.05m naoh at greater than 150 degrees, ... | 1991 | 1797397 |
| structural investigations of glucans from cultures of glomerella cingulata spaulding & von schrenck. | methylation analysis, enzymic digestion, n.m.r. spectroscopy, and smith degradation showed that the major extracellular polysaccharide, isolated from cultures of the fungus glomerella cingulata, was a (1----3)-beta-d-glucan with side chains of 1-4 (1----3)-linked beta-d-glucose residues attached to position 6. a (1----6)-beta-d-glucan was produced by the fungus in small proportions. treatment of the (1----3,1----6)-beta-d-glucan (890,315) with greater than 0.05m naoh at greater than 150 degrees, ... | 1991 | 1797397 |
| a primary demyelinating disorder of young cattle. | the clinical signs and neuropathological changes are described in six limousin x calves that, at about one month after birth, showed signs of blindness, nystagmus, rotation of the eyes, opisthotonos, hyperprotraction of the forelegs and, in one case, apparent seizures. pathologically there was necrosis of the optic chiasma and focal areas of myelin sheath vacuolation or demyelination in certain areas of the brain, especially in the cerebellar peduncles. in one animal, kept alive for 7 months, th ... | 1991 | 1800911 |
| [the heterogeneity of the alveolar macrophages of guinea pigs immunized subcutaneously with a vaccinal strain of yersinia pestis]. | the study of alveolar macrophages obtained from guinea pigs at different periods after the subcutaneous immunization with y. pestis vaccine strain has revealed the heterogeneity of these macrophages in their bactericidal activity with respect to y. pestis and in their resistance to the cytopathic action of this microorganism, as well as in the content of lysosomes and in the expression of receptors on the cell surface. the study has shown that subcutaneous immunization does not lead to the redis ... | 1991 | 1801486 |
| [the use of immunoenzyme analysis in the laboratory diagnosis of plague]. | the possibility of using the enzyme immunoassay (eia) for the early diagnosis of pneumonic plague was studied in experiments on monkeys. eia was shown to be more effective than the passive hemagglutination test. the diagnostic value of blood serum samples was found to be higher than that of nasopharyngeal mucus samples taken from the sick animals. the conclusion on the suitability of eia for the early laboratory diagnosis of this disease was made. | 1991 | 1801488 |
| the immunobiology of mycoplasma arthritidis and its superantigen mam. | | 1991 | 1802617 |
| the immunobiology of mycoplasma arthritidis and its superantigen mam. | | 1991 | 1802617 |
| [constructing a test system for the identification of plague microbe based on genetic probes]. | dna probes for detection of the plague agent yersinia pestis were made on a basis of its three typical extrachromosomal replicons. the recombinant plasmid pbs2 including pbr327 vector and salgi-bspri fragment of the plasmid pfra was constructed. the above fragment is connected with synthesis of y. pestis capsular antigen and it is a 400 bp species-specific dna probe called f1 which is suitable for identification of y. pestis species that bears the 60 mdal plasmid. the dna probes called p1 was ma ... | 1991 | 1802792 |
| [the protective effects of paeonol on cultured neonatal rat heart cells during calcium paradox]. | it has been found that the rapid (5min) and slow (120min) phases of 15ca uptake by normal myocardial cells and their beating rate are remarkably inhibited by paeonol. and that paeonol can also decrease 45ca uptake and lipid peroxide in calcium paradoxical heart cells, which appears in dose-dependence. this indicates that the mechanism of protection of paconol against calcium paradox is associated with its inhibition of ca2+ influx and antioxidation. | 1991 | 1804206 |
| cloacin tolerance and aspecific colicin insensitivity of human escherichia coli strains. | of 182 wild-type human, aerobactin producer escherichia coli strains 86.3% were insensitive to cloacin. all randomly chosen 51 strains were relatively cloacin tolerant. cloacin tolerant strains were not considerably more sensitive to hydrophobic drugs than the cloacin sensitive descendant strains. pathogenicity of the cloacin sensitive strains was significantly lower (p less than 0.05) in intraperitoneal mice infection than that of the cloacin tolerant ones. suggesting a new aspect of the uptake ... | 1991 | 1805505 |
| [connection of outer membrane protein composition in yersinia pestis cells with intrinsic plasmids]. | a set of isogenic derivatives of yersinia pestis ev strain was obtained including the variants harbouring the different compositions of yersinia own plasmids. the protein profiles of outer membranes of the set of strains were defined. the polyacrylamide gel electrophoresis has shown the small 6.1 md plasmid to code an outer membrane protein with mol mass 29 kda, different from pesticin i, while the heavy 60.0 md plasmid encodes the 15-16 kda polypeptide different from monomers of f1 and t-antige ... | 1991 | 1808507 |
| [new plasmidovars of yersinia pestis isolated in mongolia]. | the plasmid spectres of 122 strains of yersinia pestis isolated in mongolia from patients, wild mammals and arthropods were studied. the populations of three plasmidovars of yersinia pestis were found to be circulating in the natural foci of plague in mongolia. the first plasmidovar harbours three plasmids with mol masses 6, 47, 65 md. the second and third plasmidovars contain the plasmids with mol masses 6, 16, 47, 65 md and 8, 47, 75-80 md. | 1991 | 1808509 |
| tissue adhesives inhibit experimental new bone formation. | the effect of the cyanoacrylate tissue adhesive histoacryl on new bone formation was studied in rats. experimental heterotopic new bone formation was induced by implanting pieces of demineralized allogeneic bone matrix (dabm) in the abdominal wall of 10 growing sprague-dawley rats. this produces cartilage formation within and around the implants after 10 days, followed by enchondral ossification and the formation of an ossicle with remodelling bone and bone marrow after three weeks. prior to imp ... | 1991 | 1809713 |
| [comparative analysis of the 5'-terminal nucleotide sequence and central domains of 16s rrna of yersinia pestis and escherichia coli]. | partial nucleotide sequence of 16s rrna (16-989 nn.) of plague agent (yersinia pestis) was determined after sequencing of cloned cdna fragments. the comparison of y. pestis 16s rrna sequence with that of e. coli shows a number of point sequence variation due to base changes. the base changes are found in 16s rrna secondary structure regions that are localized on the surface of 30s ribosome subunit (hairpins 6 and 18) as well as in the regions that bind the proteins s8, s15, s16 and s20. these pr ... | 1991 | 1813805 |
| epidemiology of streptococcal infection with reference to rheumatic fever. | antistreptolysin antibodies were estimated in 787 normal children and young adults by latex test. this test detects titres of 200 iu/ml and above, which is the western cut off point, for diagnosis. children below one year showed no antibodies. unlike western studies where no antibodies are detected below the age of 3 years, our study revealed that 7.9% children between 1-3 years had significantly elevated antibodies. this epidemiological pattern is well reflected in the different clinical profil ... | 1991 | 1819574 |
| [anti-inflammatory activities and effect of rhizoma alismatis on immune system]. | 10 and 20 g/kg ig of rhizoma alismatis (ra) markedly decrease the clearance rate of charcoal particles in mice, but have no significant effect on the weight of immune organs, or on the content of serum antibody hemolysin and immunoglobulin g and the delayed footpad edema induced by sheep red blood cell. the contact dermatitis of mouse pinnae immunized with dinitrochlorobenzene is inhibited by ra when it is given before challenge. in addition, ra 20g/kg suppresses the swelling of mouse rinnae ind ... | 1991 | 1820797 |
| [anti-inflammatory activities and effect of rhizoma alismatis on immune system]. | 10 and 20 g/kg ig of rhizoma alismatis (ra) markedly decrease the clearance rate of charcoal particles in mice, but have no significant effect on the weight of immune organs, or on the content of serum antibody hemolysin and immunoglobulin g and the delayed footpad edema induced by sheep red blood cell. the contact dermatitis of mouse pinnae immunized with dinitrochlorobenzene is inhibited by ra when it is given before challenge. in addition, ra 20g/kg suppresses the swelling of mouse rinnae ind ... | 1991 | 1820797 |
| expression of isoforms of internal ca2+ pump in cardiac, smooth muscle and non-muscle tissues. | smooth muscle and several non-muscle tissues contain mrna for an alternative splice of the mrna for the cardiac sarcoplasmic reticulum (sr) ca,mg-atpase. based on amino acid composition deduced from cdna sequences the cardiac isoform (ic) is 110 kda while the smooth muscle and the non-muscle isoform (is) is 115 kda. this prediction in their molecular masses was tested at the protein level in rabbit stomach, aorta, uterus and vas deferens smooth muscles; stomach mucosa, brain, liver, kidney and h ... | 1991 | 1826611 |
| [specific proteolysis by fibrinolysin-coagulase from yersinia pestis of yersinia pseudotuberculosis outer membrane proteins coded by the ca(2+)-dependence plasmid]. | the pesticinogenicity 9.5 kb plasmid from yersinia pestis strain ev76 has been marked by the kanamycin phosphotransferase gene inserted into psti site and designated pp3. the obtained plasmid pp3 determines the synthesis of 45 kd pesticin, alpha and beta-forms of fibrinolysin coagulase (37 and 35 kd) and the 29, 19 and 13 kd proteins in escherichia coli mini cells. when transferred into yersinia pseudotuberculosis strain 6933 the plasmid causes the proteolysis of outer membrane proteins. the 150 ... | 1991 | 1827173 |
| occupational contact dermatitis due to acrylonitrile. | within dsm chemicals bv, a producer of acrylonitrile, skin complaints are frequent. the majority of these are of an irritant nature, while a smaller portion is based on acquired allergies. allergological examination revealed 5 employees with an allergy to acrylonitrile. 1 of these subjects also developed paraesthesiae in the skin sites affected, a finding not previously described for acrylonitrile. in the guinea pig maximization test (gpmt), acrylonitrile showed strong allergenic potential. for ... | 1991 | 1828409 |
| occupational contact dermatitis due to acrylonitrile. | within dsm chemicals bv, a producer of acrylonitrile, skin complaints are frequent. the majority of these are of an irritant nature, while a smaller portion is based on acquired allergies. allergological examination revealed 5 employees with an allergy to acrylonitrile. 1 of these subjects also developed paraesthesiae in the skin sites affected, a finding not previously described for acrylonitrile. in the guinea pig maximization test (gpmt), acrylonitrile showed strong allergenic potential. for ... | 1991 | 1828409 |
| the nature and importance of the inter-epsilon chain disulfide bonds in human ige. | ige antibodies are best known for their pathological role in allergy. the class-specific effector sites are located in the epsilon chains; these form covalent dimers via two cystine residues (cys241 and cys328) linking opposite c epsilon 2 domains. the nature and biological significance of the inter-epsilon chain disulfide-bond arrangement is unresolved. for structural and functional analysis site-specific mutations were introduced into the c epsilon 2 domain of recombinant human ige. the introd ... | 1991 | 1828428 |
| the nature and importance of the inter-epsilon chain disulfide bonds in human ige. | ige antibodies are best known for their pathological role in allergy. the class-specific effector sites are located in the epsilon chains; these form covalent dimers via two cystine residues (cys241 and cys328) linking opposite c epsilon 2 domains. the nature and biological significance of the inter-epsilon chain disulfide-bond arrangement is unresolved. for structural and functional analysis site-specific mutations were introduced into the c epsilon 2 domain of recombinant human ige. the introd ... | 1991 | 1828428 |
| [biochemical and functional characteristics of bladder muscarinic receptors and effects of experimental diabetes in rats]. | bladder dysfunction is a common complication of diabetes mellitus and is attributed in part to peripheral neuropathy. voiding function is mainly controlled by muscarinic receptor function. therefore, i investigated first the biochemical and functional characteristics of urinary bladder muscarinic receptors and then the effects of experimental diabetes on them. experimental diabetes was induced in 2 month-old male rats by intravenous injection of 65 mg/kg of streptozotocin (stz). effects of diabe ... | 1991 | 1828510 |
| [biochemical and functional characteristics of bladder muscarinic receptors and effects of experimental diabetes in rats]. | bladder dysfunction is a common complication of diabetes mellitus and is attributed in part to peripheral neuropathy. voiding function is mainly controlled by muscarinic receptor function. therefore, i investigated first the biochemical and functional characteristics of urinary bladder muscarinic receptors and then the effects of experimental diabetes on them. experimental diabetes was induced in 2 month-old male rats by intravenous injection of 65 mg/kg of streptozotocin (stz). effects of diabe ... | 1991 | 1828510 |
| oral administration of ssg, a beta-glucan obtained from sclerotinia sclerotiorum, affects the function of peyer's patch cells. | the effect of orally administered ssg, a beta-1,3-glucan obtained from the culture filtrate of a fungus, sclerotinia sclerotiorum ifo 9395, on the function of peyer's patch (pp) cells was investigated in comparison with that on spleen cells in mice. oral administration of ssg enhanced the proliferative response of pp cells to a t-cell mitogen, concanavalin a (con a), and a b-cell mitogen, lipopolysaccharide (lps), although the response of spleen cells was not affected. peyer's patch cells taken ... | 1991 | 1828793 |
| exposure to human immunodeficiency virus (hiv) type i indicated by hiv-specific t helper cell responses before detection of infection by polymerase chain reaction and serum antibodies [corrected]. | a healthy individual at risk for human immunodeficiency virus type 1 (hiv-1) infection was studied longitudinally for 19 months for evidence of exposure to and infection with hiv. peripheral blood mononuclear cells (pbmc) were tested at 1, 5, 13, 16, and 19 months for t helper (th) cell function by in vitro-generated proliferation and interleukin-2 production in response to four synthetic peptides corresponding to env of hiv. pbmc were also tested for proviral dna using gag primers by the polyme ... | 1991 | 1829105 |
| exposure to human immunodeficiency virus (hiv) type i indicated by hiv-specific t helper cell responses before detection of infection by polymerase chain reaction and serum antibodies [corrected]. | a healthy individual at risk for human immunodeficiency virus type 1 (hiv-1) infection was studied longitudinally for 19 months for evidence of exposure to and infection with hiv. peripheral blood mononuclear cells (pbmc) were tested at 1, 5, 13, 16, and 19 months for t helper (th) cell function by in vitro-generated proliferation and interleukin-2 production in response to four synthetic peptides corresponding to env of hiv. pbmc were also tested for proviral dna using gag primers by the polyme ... | 1991 | 1829105 |
| [synthesis, secretion, and proteolytic degradation of diphtheria toxin in escherichia coli]. | the recombinant plasmids have been constructed encoding the synthesis of a full-sized diphtheria toxin from its own or pr, pl-promoters of bacteriophage lambda in escherichia coli cells. the high level constitutive synthesis of toxin results in slow cell growth and plasmid elimination. the toxin was mainly detected in the periplasm, partially in the membrane and to a less extent in the cytoplasm and culturing medium. the dimeric form of toxin was found in the cytoplasm. participation of toxin b- ... | 1991 | 1836836 |
| [fleas of the eastern kyzyl kum and their epizootic significance]. | the fauna of fleas in the east kisil-kum includes 24 species. different physicogeographic regions of sands are characterized by different spectra and number of species of fleas. the greatest share in collections is composed by xenopsylla gerbilli caspica--the parasite of rhombomys opimus. in autumn the other parasite of this rodent, coptopsylla lamellifer, is a mass species. all the most significant and long changes in the number of these species are associated with changes in the density of the ... | 1991 | 1839698 |
| the use of dacron plates for dot enzyme-linked immunosorbent assay (dot-elisa). | dacron (polyethylenetherephthalate) is proposed as a matrix for dot-elisa procedures, as an alternative to nitrocellulose. plates of dacron were partially hydrazinolyzed and hydrazide groups introduced were converted to azide groups. the derivative dacron-antigen was covalently linked on to the plates through these azide groups. the derivative dacron-antigen was exaustively washed according to crook and antigen was still fixed onto the plates. protein f1a purified from yersinia pestis was used a ... | 1991 | 1842438 |
| absence of na+,k(+)-atpase regulation of endosomal acidification in k562 erythroleukemia cells. analysis via inhibition of transferrin recycling by low temperatures. | transferrin (tf) acidification has been shown to be limited to ph 6 in murine balb/c 3t3 fibroblasts, human a549 epidermoid carcinoma cells, and chinese hamster ovary cells and is followed by alkalinization during recycling. in contrast, tf acidification in the human erythroleukemic cell line k562 proceeds to below ph 5.5, and alkalinization of internal tf during recycling is not observed. to explore the regulation of endosomal ph in k562 cells, we determined whether the existence of an early en ... | 1991 | 1847374 |
| atp-dependent partitioning of the dna template into supercoiled domains by escherichia coli uvrab. | the helicase action of the escherichia coli uvrab complex on a covalently closed circular dna template was monitored using bacterial dna topoisomerase i, which specifically removes negative supercoils. in the presence of e. coli dna topoisomerase i and atp, the uvrab complex gradually introduced positive supercoils into the input relaxed plasmid dna template. positive supercoils were not produced when e. coli dna topoisomerase i was replaced by eukaryotic dna topoisomerase i or when both e. coli ... | 1991 | 1847511 |
| structure and function of the maize spm transposable element. | the spm's primary transcript is alternatively spliced to give at least 4 different transcripts which code for proteins with extensive overlapping domains. two of these proteins, the tnpa and tnpd gene products, are necessary and sufficient to promote dspm transposition in tobacco. moreover, the tnpa gene product is also sufficient to suppress the basal level of expression observed in some dspm-disrupted genes, and probably to activate the expression of inactive elements and of other dspm-disrupt ... | 1991 | 1851643 |
| chronic carcinogenic and toxic effects of a single subcutaneous dose of cadmium in the male fischer rat. | the carcinogenic effects of cadmium have not been thoroughly assessed in the commonly used fischer (f344) rat. this study determined tumor incidence in various tissues of male f344/ncr rats after a single dose of cadmium. cadmium (as cdcl2) was given sc in the dorsal thoracic midline at 30 mumole/kg to 70 8-week old male f344 rats while controls (n = 50) received saline. rats were observed during the next 90 weeks. early deaths (less than or equal to 32 weeks), due mostly to acute cadmium-induce ... | 1991 | 1855489 |
| relevance of n-nitrosamines to oesophageal cancer in china. | oesophageal cancer occurs at a very high frequency in certain areas of china, especially in linxian county, henan province. previous studies suggested that n-nitroso compounds play a causative role. in order to study further the exposure of linxian inhabitants, the intake of n-nitrosamines in the diet was determined. the total daily intake of volatile nitrosamines and of n-nitroso-n-methylbenzylamine (nmbza) was higher in linxian than in two other counties. nmbza can induce cancer in animal and ... | 1991 | 1855832 |
| participation of phenobarbital-inducible cytochrome p450 in the mutagenic activation of n-nitrosopropylamines by liver and lung 9000 g fractions from five animal species and man. | the mutagenicity of nine carcinogenic n-nitrosopropylamines was studied by the ames preincubation assay using 9000 g supernatant (s9) fractions or alcohol dehydrogenase. treatment of animals with polychlorinated biphenyls or phenobarbital resulted in a marked increase in the ability of liver s9 to activate n-nitrosobis(2-hydroxypropyl)amine, n-nitroso(2-hydroxypropyl)(2-oxopropyl)amine, n-nitrosobis(2-oxopropyl)amine, n-nitrosobis(2-acetoxypropyl)amine, n-nitroso-2,6-dimethylmorpholine, n-nitros ... | 1991 | 1855888 |
| nitrotyrosine as a new marker for endogenous nitrosation and nitration. | a sensitive and selective method has been developed for analysing 3-nitrotyrosine (nttyr), an exposure marker for exogenous and endogenous nitrosating or nitrating agents, in tissue and blood proteins by gas chromatography-thermal energy analysis. using this method, a number of kinetic studies were carried out. free and protein-bound tyrosine were reacted easily to yield nttyr. the method was also applied to the study of nttyr formation in vivo; a dose-dependent increase in nttyr was seen in bot ... | 1991 | 1855894 |
| analysis and pyrolysis of some n-nitrosamino acids in tobacco and tobacco smoke. | a new tobacco-specific nitrosamine, 4-(n-nitrosomethylamino)-4-(3-pyridyl)butyric acid (iso-nnac), has been identified in tobacco, and its structure was confirmed by gas chromatography-mass spectrometry following enrichment of a tobacco extract. the levels of iso-nnac ranged from 0.01 to 0.95 ppm. it does not induce dna repair in primary rat hepatocytes and is inactive as a tumorigenic agent in strain a mice. in order to study the fate of nitrosamino acids during smoking, we spiked cigarettes wi ... | 1991 | 1855899 |
| [the effect of yersinia pestis ev76 6 md plasmid on the composition of outer membrane proteins of yersinia pseudotuberculosis ypiii]. | on the basis of yersinia pseudotuberculosis strain ypiii the isogenic variants containing the different combinations of 47 md plasmids from yersinia pestis or yersinia pseudotuberculosis cells with the 6 md pyp plasmid from yersinia pestis ev (intact or having impaired the pla gene determining the synthesis of plasmocoagulase). the degradation of the secreted proteins encoded by the 47 md plasmids of yersinia pestis and yersinia pseudotuberculosis in the cells harbouring the 6md pyp plasmid has ... | 1991 | 1857369 |
| [the effect of a plague pathogen plasmid on lethality and immunogenicity]. | on the model of yersinia transconjugants (yersinia pestis, yersinia pseudotuberculosis, yersinia enterocolitica) carrying the conjugative cointegrates containing the 47 and 65 md plasmids from yersinia pestis the data were obtained on the different affects of the latter plasmids on the lethality and immunogenicity conferred by the host bacterial cells. the plasmid effects were drastically different during bacterial infection in mice or guinea pigs. the possibility of appearance of the recombinan ... | 1991 | 1857372 |
| [the effect of the bacterial genome on the expression and behavior of the plasmid determining the ca2+-dependence of the plague pathogen]. | the conjugative cointegrate containing the 47 md plasmid of yersinia pestis has been transferred into the strains of the different yersinia (yersinia pestis, yersinia pseudotuberculosis, yersinia enterocolitica) and escherichia coli ca. there appeared in the populations of recombinant yersinia under the conditions of ca2+ deficit at 37 degrees c the cells coming into the stasis stage or dying. it was shown on the model of yersinia enterocolitica that bacterial lethality might be prevented by exc ... | 1991 | 1857373 |
| altered lipid metabolism in parvovirus-infected cells. | a broad spectrum of cell lipid alterations are known to occur as a consequence of various viral infections. these changes include inhibition of lipid synthesis, stimulation of lipid synthesis and changes in the proportions of various lipids. the current study examined the effects of two parvoviruses on lipids of rat kidney (nrk) cells. cells were infected with h-1 or kilham rat virus (krv) and the effects on 14c-acetate incorporation determined. results showed that h-1 virus rapidly inhibited li ... | 1991 | 1865833 |
| cell migration and actin organization in cultured human primary, recurrent cutaneous and metastatic melanoma. time-lapse and image analysis. | random cell migration and actin organization in seven human primary, recurrent cutaneous, and metastatic melanoma cell lines were studied by time-lapse video recording and image analysis. the migration of over 800 randomly selected cells from the cell lines were recorded using an inverted microscope with an attached incubator housing. the fraction of cells with random migration rates greater than 10 microns/hour was 8% in an established primary melanoma cell line, 2% and 34% in two recurrent cut ... | 1991 | 1867326 |
| [radioimmunological analysis in studying the persistence of the plague antigen in the body of rodents and ectoparasites in natural foci in siberia]. | to study the persistence of y. pestis capsular antigen, or fraction 1 (f1), in the body of less important plague carriers in the mountain altai and transbaikal natural foci, as well as in experimentally infected ticks, the liquid-phase competitive radioimmunoassay (ria) was used for the first time. in this study ria showed, due to its sensitivity, doubtless advantages over traditional methods, such as the passive hemagglutination (pha) test and the antibody neutralization (an) test, and made it ... | 1991 | 1872096 |
| [the specificity of plague antigen fi in pha]. | it were inoculate a total of the 15 type and 13 group leptospira strains to the rattus flavipetus and rattus lose 137 rates in lei zhou peninsula. as the antisera acquired, it has been examined the lyso-agglutinative test in leptospira strains. up to standard serum it was to test for pha of plaque, employing the sensitized blood cell by fi from 4 antiplague institutes. all of tests are negative. in addition to test for 3 immune sera, hemorrhagic fever antisera (immunized animals apodemus agraius ... | 1991 | 1878960 |
| [cloning of the locus encoding synthesis of an outer membrane protein (omp2) of the calcium dependence plasmid of yersinia pestis (lehmann, neumann)]. | the genetic locus of yersinia pestis encoding synthesis of a 46-kda heat-inducible outer membrane protein (omp2) was cloned into pbr322 plasmid. the omp2 was shown to be analogous to previously described yoph and yop2b proteins. the fifth hindiii fragment of 48-mda calcium dependence plasmid pcad358 mediates production of 31- and 28-kda proteins, irrespective of orientation of the insertion. a 31-kda polypeptide seems to correspond to the yopj described elsewhere. the maps of bamhi and hindiii o ... | 1991 | 1879678 |
| [cyclic nucleotides, prostaglandins and thrombocyte aggregation in rats poisoned with "murine" yersinia pestis toxin]. | y. pestis "mouse" toxin, introduced intraperitoneally into rats in a dose of ld100 [correction of lg100], produces phasic changes in the thrombin-induced aggregation of thrombocytes and the content of prostaglandins and cyclic nucleotides in them. as the result of the damaging action on the endothelium of blood vessels at the initial period of intoxication, the concentration of prostaglandin 6-keto f1 alpha in blood plasma and camp [correction of camr] in thrombocytes sharply decreases, which ca ... | 1991 | 1882634 |
| acetylcholinesterase from schistosoma mansoni: immunological characterization. | the enzyme acetylcholinesterase (ache) is present in the trematode schistosoma mansoni, which infects humans and causes a severe disease called schistosomiasis or bilharzia. we have purified this enzyme and raised polyclonal antibodies against it. the specificity of these antibodies against the schistosome enzyme was demonstrated by their capacity to precipitate exclusively ache activity from cercariae extract and to recognize the 8s molecular form of the parasite's ache. on the other hand, they ... | 1991 | 1885212 |
| the protein sequence responsible for lipoprotein membrane localization in escherichia coli exhibits remarkable specificity. | structural information defining an n-terminal sequence required for the membrane sorting of bacterial lipoproteins has been previously garnered through the study of a hybrid outer membrane (om) lipo-beta-lactamase (ll) (ghrayeb and inouye (1984) j. biol. chem. 259, 463-467). introduction of an aspartate as the second residue of mature ll (d2 mutant) causes an inner membrane (im) localization of this protein (yamaguchi, k., yu, f., and inouye, m. (1988) cell 53, 423-432). introduction of as aspar ... | 1991 | 1885579 |
| effects of environmental exposure on carbon polysulphone composites. | in vivo and in vitro studies were designed to characterize the material degradation associated with implantation of carbon fibre-reinforced polysulphone (c/ps). composite plates were compression moulded for both studies, and a fibre orientation of 0 degrees with the long axis and a fibre volume fraction of approximately 55% were used. the in vitro experiment involved soaking three groups of four plates each at 4, 12 and 26 wk in 0.9% saline solution at 37 degrees c. the plates were measured for ... | 1991 | 1888812 |
| factors promoting acute and chronic diseases caused by yersiniae. | the experimental system constructed with the medically significant yersiniae provides a powerful basic model for comparative study of factors required for expression of acute versus chronic disease. the system exploits the close genetic similarity between yersinia pestis, the etiological agent of bubonic plague, and enteropathogenic yersinia pseudotuberculosis and yersinia enterocolitica. y. pestis possesses three plasmids, of which one, shared by the enteropathogenic species, mediates a number ... | 1991 | 1889045 |
| the effects of probucol on lipoprotein metabolism in the rat. | the effects of probucol on liver and intestinal apolipoprotein, ldl-receptor and hepatic lipase gene expression, as well as plasma lipid and apolipoprotein levels and liver lipase activity were evaluated in male rats. administration of probucol decreased plasma triacylglycerols, without affecting plasma cholesterol. plasma apo e and apo b concentrations increased after probucol. since liver and intestinal apo b and apo e mrna levels remained unchanged, this increase could be attributed to a dela ... | 1991 | 1892873 |
| cytogenesis in the monkey retina. | time of cell origin in the retina of the rhesus monkey (macaca mulatta) was studied by plotting the number of heavily radiolabeled nuclei in autoradiograms prepared from 2- to 6-month-old animals, each of which was exposed to a pulse of 3h-thymidine (3h-tdr) on a single embryonic (e) or postnatal (p) day. cell birth in the monkey retina begins just after e27, and approximately 96% of cells are generated by e120. the remaining cells are produced during the last (approximately 45) prenatal days an ... | 1991 | 1894769 |
| identification and cloning of a fur regulatory gene in yersinia pestis. | yersinia pestis is one of many microorganisms responding to environmental iron concentrations by regulating the synthesis of proteins and an iron transport system(s). in a number of bacteria, expression of iron uptake systems and other virulence determinants is controlled by the fur regulatory protein. dna hybridization analysis revealed that both pigmented and nonpigmented cells of y. pestis possess a dna locus homologous to the escherichia coli fur gene. introduction of a fur-regulated beta-ga ... | 1991 | 1898928 |
| 5-enolpyruvylshikimate-3-phosphate synthase from escherichia coli--the substrate analogue bromopyruvate inactivates the enzyme by modifying cys-408 and lys-411. | in order to identify the essential reactive amino acid residues of 5-enolpyruvylshikimate-3-phosphate synthase, the reaction of the enzyme with its substrate analogue bromopyruvate was investigated. incubation of the enzyme with bromopyruvate resulted in a time-dependent loss of enzyme activity. the inactivation followed pseudo-first-order and saturation kinetics with a kinact of 28 microm and a maximum rate constant of 0.31 min-1. the inactivation was prevented by preincubation of the enzyme wi ... | 1991 | 1899181 |
| introduction of vascular smooth muscle cells expressing recombinant genes in vivo. | vascular smooth muscle cells contribute to the formation of atherosclerotic plaques by proliferating in response to vascular injury and releasing growth-promoting factors. because their autocrine and paracrine effects are not fully understood, expression of such growth factor genes in specific cell types in vivo would help to determine their mechanism of action. we describe a method to transfer vascular smooth muscle cells expressing recombinant gene products to localized segments of the arteria ... | 1991 | 1899366 |
| the yersinia pestis v antigen is a regulatory protein necessary for ca2(+)-dependent growth and maximal expression of low-ca2+ response virulence genes. | the low-ca2+ response is a multicomponent virulence regulon of the human-pathogenic yersiniae in which 12 known virulence genes are coordinately regulated in response to environmental cues of temperature, ca2+, and nucleotides such as atp. yersinial growth also is regulated, with full growth yield being permitted at 37 degrees c only if ca2+ or a nucleotide is present. in this study, we constructed and characterized a mutant yersinia pestis specifically defective in the gene encoding the v antig ... | 1991 | 1901573 |
| tumor-targeted delivery of 8-hydroxyquinoline. | rif-1 mouse tumors express high levels of beta-glucuronidase activity relative to most normal tissues. the high activity can be exploited for targeting specific drugs preferentially to tumor tissues. in this study we examined the kinetics of 8-hydroxyquinoline (8-ohq) accumulation in tumor and in several normal tissues resulting from the in vivo deconjugation of 8-hydroxyquinolyl-glucuronide (8-ohq-glca). tumors were acidified with d-glucose and nahco3 prior to the administration of 8-ohq-glca; ... | 1991 | 1904410 |
| thyroid vascular conductance: differential effects of elevated plasma thyrotropin (tsh) induced by treatment with thioamides or tsh-releasing hormone. | we have reported previously that thyroid gland blood flow, expressed as vascular conductance (c) per mass, is decreased at very low and increased at very high chronic plasma tsh concentrations, but is apparently unchanged over a broad range of plasma tsh concentrations encompassing normal levels. the aim of the present study was to examine the apparently very steep dose-response relationship between elevated plasma tsh and thyroid vascular c/mass. in the first series of experiments, endogenous p ... | 1991 | 1905224 |
| [study of the dependence of gene expression of plague pathogen plasmid 65md on the host bacterium genome]. | the conjugative cointegrate containing yersinia pestis 65 md plasmid coding for the production of traction i antigen and mouse toxin has been transferred into yersinia pseudotuberculosis, yersinia enterocolitica and escherichia coli cells. analysis of the transconjugants obtained has confirmed the connection of the genetical determinants for the mentioned bacterial products with the 65 md plasmid. the similar level of fra and tox-genes expression has been found in yersinia cells while fraction i ... | 1991 | 1906986 |
| clinical testing of thermally stimulated cardiovascular oscillations in man. | the study assessed the physiological validity of an automatic thermal stimulation method to induce synchronised oscillations in the neural cardiovascular control system. | 1991 | 1913758 |
| cloning, sequencing, and expression of the p-protein gene (phea) of pseudomonas stutzeri in escherichia coli: implications for evolutionary relationships in phenylalanine biosynthesis. | the phea gene encoding the bifunctional p-protein (chorismate mutase:prephenate dehydratase) was cloned from pseudomonas stutzeri and sequenced. this is the first gene of phenylalanine biosynthesis to be cloned and sequenced from pseudomonas. the phea gene was expressed in escherichia coli, allowing complementation of an e. coli phea auxotroph. the enzymic and physical properties of the p-protein from a recombinant e. coli auxotroph expressing the phea gene were identical to those of the native ... | 1991 | 1919506 |
| experimental infection of domestic ferrets (mustela putorius furo) and siberian polecats (mustela eversmanni) with yersinia pestis. | eight domestic ferrets (mustela putorius furo) and two siberian polecats (m. eversmanni) were inoculated subcutaneously with 12 to 1.2 x 10(7) yersinia pestis originally isolated during an epizootic of plague in white-tailed prairie dogs (cynomys leucurus) near meeteetse, park county, wyoming (usa) in 1985. none of the ferrets or polecats developed clinical signs of disease which suggested that black-footed ferrets (m. nigripes), a congener, also would be resistant to plague. all animals receivi ... | 1991 | 1920664 |
| [the effect of extradurally administered analgesics on somatosensory evoked eyelid microvibration in rabbits]. | we examined the effect of various analgesics administered lumboextradurally or intravenously on acceleration of the eyelid microvibration (emv) due to reflex contraction which was evoked by electrical stimulation of the sciatic and ulnar nerve in rabbits. no significant difference in the inhibition of emv was observed initially between the sciatic nerve and the ulnar nerve stimulated group after 2 mg of morphine, administered either extradurally or intravenously. however, greater inhibition of e ... | 1991 | 1920786 |
| hepatocyte hepatitis b surface antigen expression in chronic hepatitis b virus carriers in singapore: correlation with viral replication and liver pathology. | the hepatocyte hepatitis b surface antigen (hbsag) expression in 149 liver biopsies from 124 chronic hepatitis b virus (hbv) carriers was correlated with serum hbv dna status and histologic activity. hepatocyte hbsag was stained by the peroxidase-antiperoxidase method and serum hbv dna was determined by dot blot hybridization. sixty-five biopsies (44%) showed minimal changes (mc), 82 biopsies (55%) showed chronic liver disease (cld) and 2 biopsies (1%) showed hepatocellular carcinoma. hepatocyte ... | 1991 | 1932667 |
| alteration in bone marrow adherent layer growth factor expression: a novel mechanism of chronic myelogenous leukemia progression. | philadelphia chromosome1 positive (ph1) chronic myelogenous leukemia (cml) is characterized by metamorphosis of the chronic phase to blastic crisis. however, cellular events associated with this transition are poorly understood. to examine the possible participation of hematopoietic growth factors in this process, we studied growth factor expression in adherent layers of bone marrows derived from cml ph1 patients in various stages of the disease. interleukin-1 beta (il-1 beta) and il-6 mrna were ... | 1991 | 1932751 |
| a case of ocular tilt reaction and torsional nystagmus due to direct stimulation of the midbrain in man. | a case is presented of a patient who underwent insertion of a periaqueductal grey electrode for the relief of chronic pain. shortly after insertion, the patient developed a left-sided ocular tilt reaction (otr). the electrode tip, initially to the right of the midline, was then withdrawn slightly so that it was now on the left side. stimulation at this point gave rise to a worsening of the tilt reaction, in addition to bilateral counter-clockwise torsional nystagmus. computerized tomography and ... | 1991 | 1933234 |
| effect of a nonpeptide angiotensin ii receptor antagonist, dup 753, on angiotensin-related water intake in rats. | the effect of peripheral administration of the nonpeptide angiotensin ii-1 (aii) receptor blocker, dup 753, on the dipsogenic responses to peripherally administered angiotensins i, ii, and iii was tested. in all cases, dup 753 significantly inhibited the drinking response, whether administered 15 or 45 minutes prior to administration of the dipsogen. these results suggest that the drinking responses to angiotensins i, ii, and iii are mediated by aii-1 receptors. they also suggest that either aii ... | 1991 | 1933441 |
| drug use during breast-feeding. | the physicochemical and pharmacokinetic factors involved in transfer of drugs into breast milk are reviewed. passage of drugs into milk can best be viewed as a two-compartment system. various ratios of the drug concentration values in the two compartments and ratios of maternal-to-infant intake have been described. knowledge of the limitations of these methods is necessary to properly interpret and apply the literature on drug excretion into breast milk. factors involved in choosing a drug for a ... | 1991 | 1934918 |
| the v antigen of yersiniae: an overview. | | 1991 | 1935079 |
| chromosomal marker for the 'high pathogenicity' phenotype in yersinia. | | 1991 | 1935090 |
| preparation of monoclonal antibody to v antigen from yersinia pestis. | | 1991 | 1935094 |
| persistent survival of attenuated yersinia pestis organisms with coexistence of chondroitin sulfate colloidal iron within guinea pig macrophages as an initial step of the induction of potent antiplague immunogenesis. | | 1991 | 1935095 |
| the cell cycle-regulated flagellar gene flbf of caulobacter crescentus is homologous to a virulence locus (lcrd) of yersinia pestis. | we have characterized flbf, a key locus located at the top of the flagellar gene hierarchy of caulobacter crescentus. this gene is required for transcription from sigma 54 promoters of fla genes expressed late in the cell cycle. we have determined the nucleotide sequence of the gene, mapped the 5' end of the flbf rna, and examined the pattern of expression in the cell cycle. our results show that flbf is expressed earlier in the cell cycle than other fla genes, that it is expressed at a low leve ... | 1991 | 1938923 |
| four-week inhalation exposures of rats to aerosols of three lubricant base oils. | sprague-dawley rats were exposed to aerosols of one of three base stocks used to formulate lubricating oils. these stocks were a solvent-refined oil (sro), a hydrotreated and acid-washed white oil (wto) and a severely hydrotreated and hydrocracked oil (hbo). exposures were for 6 h per day, 5 days per week for ca. 4 weeks. there were four groups of rats for each study (10 per sex per group). aerosol concentrations were ca. 0, 50, 210 and 1000 mg m-3 for each material; the mass median aerodynamic ... | 1991 | 1940005 |
| image cytometry of progesterone receptor expression during the cell cycle in the mcf-7 cell line. | progesterone receptors (pr) appear to be distributed in a heterogeneous way in mammary tumor cells. the study presented here was designed to examine if heterogeneity of pr expression is cell-cycle dependent. immunofluorescence techniques were used to label pr on the mcf-7 human breast cancer cell line and image cytometry was used to analyze the pr expression during g0 (ki-67 antigen-negative cells), g1, s, and g2/m cell-cycle phases. a second pr, brdu, and dna analysis was performed to study pr ... | 1991 | 1940323 |
| [cytological examination of urethral and endocervical smears in the diagnosis of infections]. | cytologic studies of smears from 130 women suffering from various diseases, including 61 pregnant patients with gestoses, have shown structural changes of the cells in the smears from the endocervix and urethra, that permitted the diagnosis of mycoplasma infection in 43.8% of patients, chlamydia infection, 52.3%, and herpes simplex in 72.3% of patients; in 63.9% of patients these infections were combined. cytologic studies may be useful in the tentative diagnosis of urogenital infections, includ ... | 1991 | 1951977 |
| angiosarcoma in postsurgical lymphedema. an unusual occurrence in a man. | a 63-year-old man underwent partial left axillary node dissection for metastatic melanoma of unknown primary. lymphedema of his left upper extremity developed after surgery. eight years later, extensive cutaneous angiosarcoma developed in the edematous limb. the occurrence of cutaneous angiosarcoma in postsurgical lymphedema is extremely rare in men; to our knowledge, our case is the fifth such case thus far reported. | 1991 | 1951986 |
| [the ozone layer and its modification by n2o and inhalation anesthetics]. | as a result of human activities the ozone layer in the stratosphere, which is necessary for life on earth, has changed. the main causes of ozone destruction are chlorofluorcarbons (cfcs) 11 and 12. recently, caring anesthetists have wondered if and to what degree n2o and popular potent inhalation anesthetics may also contribute to ozone loss. having consulted the literature, we attempt to answer that question. the ozone-destroying n2o is chiefly produced by burning fossil elements and nitrogenou ... | 1991 | 1952035 |