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normal early pregnancy: serum hcg levels and vaginal ultrasonography findings.vaginal sonography and determination of serum hcg levels were carried out in 22 healthy pregnant women every 2 to 4 days after the first positive pregnancy test until a living fetus was observed. gestational age was calculated from the day of ovulation, assessed by lh surge or hcg administration, plus 14 days. a gestational sac of 1-3 mm was detected at a mean (sem) of 31.2 (0.2) days of gestation (range 30-33 days). the corresponding mean hcg level was 730 iu/l (30) and the range 467-935 iu/l ( ...20072223680
tissue integration of a12o3-ceramic dental implants (frialit)--a case report.the tissue intraosseous integration of a12o3-ceramic dental implants (frialit) is studied in a patient case, where an implant-fracture made it necessary to remove the implant from a patient's jaw. the surgical removing of the apical part of the implant with the use of a trepan bur made it possible to examine and visualize histologically and microradiographically the tissue adjacent to the implant. the results of both the histological and microradiographical studies showed that a good healing and ...20072374995
bacteriological methods in diagnosis of tuberculous meningitis. 20073771495
esophageal function and dysfunction. 20071951923
distinction of mesothelioma from carcinoma in pleural effusions. an immunocytochemical study on routinely processed cytoblock preparations.the study was designed to find out whether the commercially available antibodies bma 130 c, bma 120, v 9, kl 1, b 72.3 tag, hea 125 and ber-ep 4 would be of help in distinguishing carcinomas from a mesothelial process (mesothelioma/pleuritis) in pleural effusion specimens routinely processed by the cytoblock method. all of the 20 carcinomas included in the study but also 19 of the 20 mesotheliomas expressed cytokeratin (kl 1), whereas vimentin expression was found in 7 of the 20 carcinomas and 1 ...20071876528
body mass index variations: centiles from birth to 87 years.this report provides body mass index (weight/height2) values for the french population from birth to the age of 87 years. bmi curves increase during the first year, decrease until the age of 6, increase again up to 65 years and decrease thereafter. these variations reflect the total changes of fat body mass during life. the 50th centile values of wt/ht2 at the ages of 20, 40, 60, 80 years are 21.5, 24.6, 25.4, 24.4 kg/m2 for men and 20.6, 22.6, 24.1, 23.4 kg/m2 for women. the values for the 3rd, ...20071855495
high-voltage electrical injury: acute pathophysiology.a reproducible high-voltage electrical injury model was established in the primate using a new approach to energy administration, measurement instrumentation, and data acquisition. patterns of current repartition and temperature generation were examined in 24 primates. the predominant current load was carried in muscle, which is the tissue group occupying the largest cross-sectional area. highest temperature values observed were in muscles of small cross-sectional diameter and in tissues of high ...20073351227
role of ceramide as a lipid mediator of 1 alpha,25-dihydroxyvitamin d3-induced hl-60 cell differentiation.the treatment of hl-60 myelocytic leukemia cells with 1 alpha,25-dihydroxyvitamin d3 (1,25-(oh)2d3) resulted in the activation of a neutral sphingomyelinase and in sphingomyelin turnover (okazaki, t., bell, r., and hannun, y. (1989) j. biol. chem. 264, 19076-19080). in this paper, the effects of 1,25-(oh)2d3 on the product of sphingomyelin hydrolysis, ceramide, and the possible function of ceramide as a lipid mediator of the effects of 1,25-(oh)2d3 on hl-60 cell differentiation were investigated ...20072394750
increased vitamin e intake restores fish-oil-induced suppressed blastogenesis of mitogen-stimulated t lymphocytes.we sought to determine whether fish-oil supplementation would suppress blastogenesis in vitro of concanavalin a (cona)-stimulated peripheral blood mononuclear cells (pbmcs) and, if so, whether it could be reversed with increased intake of vitamin e. healthy males ate a controlled basal diet providing a total of 40% of energy from fat when fed in conjunction with 15 g/d of either placebo oil (po) or fish-oil concentrate (foc) fortified with 15 mg alpha-tocopherol/d for three periods. the subjects ...20071951163
myopic excimer laser keratectomy. a preliminary report. 20071936430
residual effectiveness of insecticides for control of german cockroaches (dictyoptera: blattellidae) in food-handling establishments.residual effectiveness of insecticides was compared for two treatment methods at various sites within two commercial kitchens. for all sites and insecticides, german cockroach, blattella germanica (l.), mortality was greatest for treatments of cracks and crevices than for treatments of exposed surfaces. mortality at the dishwashing site was lower than at all other sites. we attributed reduced mortality at the dishwashing site to a higher vapor pressure deficit. mortality and the amount of insect ...20072258513
residual effectiveness of insecticides for control of german cockroaches (dictyoptera: blattellidae) in food-handling establishments.residual effectiveness of insecticides was compared for two treatment methods at various sites within two commercial kitchens. for all sites and insecticides, german cockroach, blattella germanica (l.), mortality was greatest for treatments of cracks and crevices than for treatments of exposed surfaces. mortality at the dishwashing site was lower than at all other sites. we attributed reduced mortality at the dishwashing site to a higher vapor pressure deficit. mortality and the amount of insect ...20072258513
the electrocardiogram of european shrews.a recording method of electrocardiograms in resting animals is described. the electrocardiograms of crocidura russula, crocidura leucodon, crocidura suaveolens, neomys fodiens and sorex araneus are similar to those of other small mammals. t-wave directly follows the qrs-complex. the duration of electrocardiograms depends on heart mass and body temperature. heart rate shows no influence on the duration of electrocardiograms.20072870871
the electrocardiogram of european shrews.a recording method of electrocardiograms in resting animals is described. the electrocardiograms of crocidura russula, crocidura leucodon, crocidura suaveolens, neomys fodiens and sorex araneus are similar to those of other small mammals. t-wave directly follows the qrs-complex. the duration of electrocardiograms depends on heart mass and body temperature. heart rate shows no influence on the duration of electrocardiograms.20072870871
olfactory dysfunction in parkinson's disease.an olfactory function test in 18 patients with parkinson's disease (pd) and 10 age-matched control subjects was performed. both detection and recognition thresholds were measured with five kinds of synthesized odorants (t & t olfactometry). before each test, rhinoscopic inspections were performed to exclude subjects who could have respiratory hyposmia. both detection and recognition thresholds in patients with pd were significantly elevated in comparison with those of control subjects. auditory ...20071852411
[glycogenosis type ii (pompe's disease) in a 6-month-old child]. 20072780149
replantation in children.the authors have replanted 162 parts in 120 children over the past 15 years. the youngest patient, undergoing successful replantation, was aged 7 months, 3 weeks. unlike an adult, any child suffering a traumatic amputation should be considered for a possible replantation. replantation should consist of minimal bone shortening to preserve epiphyseal plates, with repair of all severed structures. longitudinal k-wires usually provide adequate fixation. our survival rate for complete replantation in ...20071865815
[social health in east germany]. 20071828554
[maternal morbidity and mortality in extra-genital pathology].the authors analyze the incidence and structure of acute and chronic visceral diseases in pregnant women and the incidence and structure of maternal death because of extragenital diseases. causes of maternal mortality are discussed and approaches to reducing maternal morbidity and mortality in visceral diseases outlined.20071951974
vecuronium infusion requirements in pediatric patients during fentanyl-n2o-o2 anesthesia.eighty-one pediatric patients, ranging from neonates to adolescents, were studied during fentanyl-n2o-o2 anesthesia to determine for each of them the vecuronium infusion required to maintain 90-95% neuromuscular block (nmb). electromyographic monitoring with train-of-four stimuli was used. the steady infusion rate was 62 +/- 15 (sd) micrograms.kg-1.hr-1 in neonates and infants. this rate was 40% of that required by children 3 to 10 years old (154 +/- 49 micrograms.kg-1.hr-1; p less than 0.05). i ...20072562909
the company they keep. fifth annual meeting on oncogenes sponsored by the foundation for advanced cancer studies, inc., frederick, md, usa, june 27-july 1, 1989. 20072577367
the company they keep. fifth annual meeting on oncogenes sponsored by the foundation for advanced cancer studies, inc., frederick, md, usa, june 27-july 1, 1989. 20072577367
inhibition by anti-cd2 monoclonal antibodies of anti-cd3-induced t cell-dependent b cell activation.anti-cd3 mab can activate t cells to help in b cell activation as detected by late events, such as maturation of b cells into ig-secreting cells (igsc), or by early events, such as b cell surface expression of the activation marker cd23. two different anti-cd2 mab each inhibited anti-cd3-induced t cell-dependent b cell activation in a dose-dependent fashion. neither irradiation of the t cells prior to culture nor depletion of cd8+ cells abrogated the inhibitory effects of anti-cd2 mab. despite t ...20071976440
[derivative spectroscopy and its use in sanitary hygiene control].an analytical method is developed for defining the residual quantities of rodenticide "brodifacoum" (talon) used in the control of harmful field rodents and especially for species microtus. the method is based on extracting the pesticide with chloroform and making use of spectrophotometry in the ultraviolet field in intervals 340-240 nm. the second derivative of the absorption is utilized as well as the band with maximum 288 nm and minimum 228 nm. the peak height in this interval is in proportio ...20072099444
[current status of multi-drug resistant bacteria in infectious diarrheal diseases and its control]. 200717455674
effects of heating, storage, and ultraviolet exposure on antimicrobial activity of garlic juice.this study was designed to investigate the effect of heating, storage, and ultraviolet exposure on antimicrobial activity of garlic juice and its bacteriocidal activity against common human pathogens. antimicrobial activity of fresh garlic juice was tested against escherichia coli, staphylococcus aureus, streptococcus hemolyticus b, s. hemolyticus a, klebsiella sp., shigella dysenteriae, and candida albicans using the disc method. the dilution method was performed by addition of garlic juice to ...200717472490
protective role of lactobacilli in shigella dysenteriae 1-induced diarrhea in rats.studies on lactic acid bacteria exemplify their use against various enteropathogens in vitro. nevertheless, in vivo effects of lactobacillus during shigella infection have not been evaluated. the present study evaluated the effect of lactobacillus rhamnosus and lactobacillus acidophilus on neutrophil infiltration and lipid peroxidation during shigella dysenteriae 1-induced diarrhea in rats.200717483010
emergence of shiga toxin 1 genes within shigella dysenteriae type 4 isolates from travelers returning from the island of hispañola.shiga toxins are produced by shigella dysenteriae type 1 and certain strains of escherichia coli. three cases of shiga toxin-producing s. dysenteriae type 4 were identified among travelers to the island of hispañola between 2002 and 2005. clinical and public health practitioners should be aware of this newly identified strain.200717556630
close relation of the o-polysaccharide structure of escherichia coli o168 and revised structure of the o-polysaccharide of shigella dysenteriae type 4.the o-polysaccharide was isolated from the lipopolysaccharide of escherichia coli o168 and studied by chemical analyses and smith degradation along with (1)h and (13)c nmr spectroscopies. the following structure of the branched pentasaccharide repeating unit of the o-polysaccharide was established: [carbohydrate structure: see text] where 6-o-acetylation of glcnac is partial. reinvestigation of the o-polysaccharide of shigella dysenteriae type 4 established earlier showed it to have the same str ...200717880932
a case of shigellosis with intractable septic shock and convulsions.although shigellosis is a potentially fatal disease that may cause a number of extra-intestinal manifestations, intractable septic shock is an unusual complication. here we describe a 6-month-old infant who developed severe septic shock and convulsions during an episode of dysentery caused by multidrug-resistant shigella dysenteriae. the case presentation demonstrates how shigellosis can lead to rare and potentially misleading complications such as septic shock when not treated adequately and pr ...200717881877
a new alpha-galactosyl-binding protein from the mushroom lyophyllum decastes.a new alpha-galactosyl binding lectin was isolated from the fruiting bodies of the mushroom lyopyllum decastes. it is a homodimer composed of noncovalently-associated monomers of molecular mass 10,276da. the lectin's amino acid sequence was determined by cloning from a cdna library using partial sequences determined by automated edman sequencing and by mass spectrometry of enzyme-derived peptides. the sequence shows no significant homology to any known protein sequence. analysis of carbohydrate ...200717904093
biochemical characterization of dtdp-d-qui4n and dtdp-d-qui4nac biosynthetic pathways in shigella dysenteriae type 7 and escherichia coli o7.o-antigen variation due to the presence of different types of sugars and sugar linkages is important for the survival of bacteria threatened by host immune systems. the o antigens of shigella dysenteriae type 7 and escherichia coli o7 contain 4-(n-acetylglycyl)amino-4,6-dideoxy-d-glucose (d-qui4nglyac) and 4-acetamido-4,6-dideoxy-d-glucose (d-qui4nac), respectively, which are sugars not often found in studied polysaccharides. in this study, we characterized the biosynthetic pathways for dtdp-d-q ...200717905981
holo- and apo-bound structures of bacterial periplasmic heme-binding proteins.an essential component of heme transport in gram-negative bacterial pathogens is the periplasmic protein that shuttles heme between outer and inner membranes. we have solved the first crystal structures of two such proteins, shut from shigella dysenteriae and phut from pseudomonas aeruginosa. both share a common architecture typical of class iii periplasmic binding proteins. the heme binds in a narrow cleft between the n- and c-terminal binding domains and is coordinated by a tyr residue. a comp ...200717925389
shiga toxin induces tubular membrane invaginations for its uptake into cells.clathrin seems to be dispensable for some endocytic processes and, in several instances, no cytosolic coat protein complexes could be detected at sites of membrane invagination. hence, new principles must in these cases be invoked to account for the mechanical force driving membrane shape changes. here we show that the gb3 (glycolipid)-binding b-subunit of bacterial shiga toxin induces narrow tubular membrane invaginations in human and mouse cells and model membranes. in cells, tubule occurrence ...200718046403
[identification of closely related bacteria via phylogenetic methods].to differentiate closely related pathogenic bacteria via phylogenetic method on the basis of gyrb gene sequences.200718067224
in vitro activity of cefadroxil, cephalexin, cefatrizine and cefpirome in presence of essential and trace elements.evidences supporting the introduction of metallic elements in several biological processes are rapidly accumulating. likewise, many drugs possess modified toxicological and pharmacological properties when in the form of metal complexes. in order to ascertain the role of various essential and trace element complexation on the antibacterial activity of various cephalosporins, the synergistic or antagonistic behavior of cefadroxil, cephalexin, cefatrizine and cefpirome in presence of essential and ...200717604254
core-linked lps expression of shigella dysenteriae serotype 1 o-antigen in live salmonella typhi vaccine vector ty21a: preclinical evidence of immunogenicity and protection.shigella dysenteriae serotype 1 (s. dysenteriae 1) causes severe shigellosis that is typically associated with high mortality. antibodies against shigella serotype-specific o-polysaccharide (o-ps) have been shown to be host protective. in this study, the rfb locus and the rfp gene with their cognate promoter regions were pcr-amplified from s. dysenteriae 1, cloned, and sequenced. deletion analysis showed that eight rfb orfs plus rfp are necessary for biosynthesis of this o-ps. a tandemly-linked ...200717629369
brucella abortus requires the heme transporter bhua for maintenance of chronic infection in balb/c mice.the gene annotated bab2_1150 in the brucella abortus 2308 genome sequence is predicted to encode a homolog of the well-characterized heme transporter shua of shigella dysenteriae and accordingly has been given the designation bhua (brucella heme utilization). phenotypic analysis of an isogenic bhua mutant derived from b. abortus 2308 verified that there is a link between bhua and the ability of the parent strain to use heme as an iron source in in vitro assays. maximum expression of bhua in b. a ...200717709407
effect of the nonreducing end of shigella dysenteriae type 1 o-specific oligosaccharides on their immunogenicity as conjugates in mice.endemic and epidemic shigellosis, an acute invasive disease of the lower intestines, afflicts millions of people worldwide with an estimated one million fatalities per annum at a low infectious dose. our approach to vaccine development against shigella is based on the hypothesis that serum igg antibodies to the o-specific polysaccharide (o-sp) domains of the lps of these organisms confer protection to infection. the synthetic oligosaccharides corresponding to the tetrasaccharide repeating unit o ...200717726093
[effects of huoxiang zhengqi liquid on enteric mucosal immune responses in mice with bacillus dysenteriae and salmonella typhimurium induced diarrhea].to explore the effects of huoxiang zhengqi liquid (hxzq) on enteric mucosal immune responses in mice with bacillus dysenteriae and salmonella typhimurium induced diarrhea (bsd).200718257268
shigella isolates of nepal: changes in the incidence of shigella subgroups and trends of antimicrobial susceptibility pattern.shigellosis is an important cause of bloody diarrhoea in all age groups, especially in children. a retrospective study was done to analyse the pattern of shigella isolates and the antimicrobial susceptibility trend of these shigella isolated at different hospitals of nepal from jan, 2003- dec, 2005.200718603983
prevalence of acute diarrhoea in kathmandu valley.this retrospective study was conducted during january to september in the year 1997. three hundred and forty nine stool samples were collected from diarrhoea patients from different places of kathmandu valley and examined at national public health laboratory (nphl), teku, kathmandu. acute diarrhoea becomes epidemic in rainy season and is a major public health problem of the city. in this study, people with poor hygiene practice and poor education were infected more than other people. among the 3 ...200718340369
antibacterial activity of some 3-(arylideneamino)-2-phenylquinazoline-4(3h)-ones: synthesis and preliminary qsar studies.synthesis of ten 3-(arylideneamino)-2-phenylquinazoline-4(3h)-ones is reported. all the compounds contained a common phenyl group at the 2-position, while the substituents on the arylideneamino group were varied. the compounds were investigated for their antimicrobial activity against both gram-positive (staphylococcus aureus 6571 and bacillus subtilis) and gram-negative bacteria (escherichia coli k12 and shigella dysenteriae 6) using a turbidometric assay method. it was found that the incorpora ...200717978766
antimicrobial susceptibility of shigella flexneri and s. dysenteriae isolated from stool specimens of patients with bloody diarrhoea in mwanza, tanzania.this study was conducted to determine frequency and pattern of antimicrobial susceptibility of shigella species isolated from stool specimens collected from patients presenting with bloody diarrhoea in mwanza city, tanzania. the study was carried out from october 2004 to october 2005 and involved patients attending sekou toure regional hospital and butimba health centre. bacteriological cultures were done at the national institute for medical research laboratory. a total of 489 patients (median ...200718087897
parathyroid gland disease and hypercalcemia. 20072223448
parathyroid gland disease and hypercalcemia. 20072223448
methotrexate as an adjunct in the treatment of persistent mild cardiac allograft rejection.because methotrexate arrests inflammation in autoimmune disease, we studied its efficacy in persistent low-grade cardiac allograft rejection. seventeen patients aged 39.5 +/- 0.9 years (mean +/- se) had persistent rejection despite previous therapy with high dose corticosteroids. maintenance immunosuppression consisted of prednisone, azathioprine, and cyclosporine. the rejection episode treated with methotrexate occurred 180 +/- 55.4 days posttransplantation. patients had incurred 2.7 +/- 0.3 pr ...20072238053
methotrexate as an adjunct in the treatment of persistent mild cardiac allograft rejection.because methotrexate arrests inflammation in autoimmune disease, we studied its efficacy in persistent low-grade cardiac allograft rejection. seventeen patients aged 39.5 +/- 0.9 years (mean +/- se) had persistent rejection despite previous therapy with high dose corticosteroids. maintenance immunosuppression consisted of prednisone, azathioprine, and cyclosporine. the rejection episode treated with methotrexate occurred 180 +/- 55.4 days posttransplantation. patients had incurred 2.7 +/- 0.3 pr ...20072238053
[anxiety and the presence of the father at childbirth].the authors give a report of a follow-up the anxiety of 363 couples whereby the husband decided to be present at the childbirth and took part in the childbirth preparation courses. the anxiety state and anxiety trait in men was found to be significantly lower than in women. the data of women were compared to a matched control group consisting of 135 pregnant women. in cases where the husband was present at childbirth the anxiety state and anxiety trait of the women was considerably lower than in ...20072652033
nonsquamous tumors of the head and neck in the geriatric population.thyroid tumors, salivary gland tumors, and non-hodgkin's lymphoma are described. psychosocial issues involving the geriatric patient are discussed. case reports of a number of geriatric tumor patients are included.20072074986
insulin and blood pressure regulation.epidemiological evidence suggests that there is a close association between obesity, non-insulin-dependent diabetes (niddm) and hypertension. obesity and niddm are the classical insulin-resistant states. even in the absence of these conditions, essential hypertension is associated with insulin resistance. in view of the acute effects of insulin on renal sodium reabsorption, the sympathetic nervous system, the renin-angiotensin-aldosterone system, the transmembranous cation transport, the cardiov ...20072043223
retention of a functional resact receptor in isolated sperm plasma membranes.resact, a peptide obtained from eggs, causes a change in the mr, and a loss of 32p from a plasma membrane protein identified as guanylate cyclase. here, a resact analog (125i-[tyr1, ser8] resact) was synthesized and shown to bind to isolated sperm membranes. resact, but not speract, competed with the radiolabeled ligand for binding. when membranes were prepared under appropriate conditions, guanylate cyclase remained at mr 160,000; the incubation of membranes with gamma-32p-atp resulted in the f ...20072870813
retention of a functional resact receptor in isolated sperm plasma membranes.resact, a peptide obtained from eggs, causes a change in the mr, and a loss of 32p from a plasma membrane protein identified as guanylate cyclase. here, a resact analog (125i-[tyr1, ser8] resact) was synthesized and shown to bind to isolated sperm membranes. resact, but not speract, competed with the radiolabeled ligand for binding. when membranes were prepared under appropriate conditions, guanylate cyclase remained at mr 160,000; the incubation of membranes with gamma-32p-atp resulted in the f ...20072870813
inner city psychiatry. 20071932958
which patients see a rheumatologist? swracr (south western regional advisory committee on rheumatology).rheumatology outpatient consultations were recorded throughout the south western regional health authority for one month. of 2987 consultations (equivalent to 32,600 annually), 630 (21%) were new referrals who waited 60 days (mode) before their consultation. rheumatoid arthritis and polyarthritis accounted for 43% of new referrals and 75% of follow-up patients. for these categories, 6.5 follow-up patients were seen for each new referral, but this ratio varied from 3.7 to 11.7. other diagnoses al ...20071863825
effectiveness of fortnightly tooth brushing with amine fluorides in caries-prone subjects.the aim of this study was to assess the caries incidence and plaque accumulation in schoolchildren at caries risk, after brushing the teeth fortnightly with gels containing 0, 0.4% f, 1.25% f as amine fluoride (amf) or the common amine fluoride toothpaste containing 0.125% f. the study was conducted double blind over an 18-month period, and after 6 months discontinuation of brushing. only the group that brushed with the 1.25% amf gel showed a significant decrease in caries development compared t ...20071890532
development of nephrology nursing care in europe: 1978-1988.nephrology nursing care in europe has been accelerated by medical and technical advances. nurses are becoming more and more involved in decision-making processes as opposed to the traditional serving role. this article analyzes some aspects responsible for these changes in europe from 1978-1988 and how nephrology nurses adapted to these developments.20072658872
[infectious endocarditis]. 20071855806
perfusion characteristics of hepatic cavernous hemangioma using intravenous ct angiography (ivcta).thirty-three patients with 35 proven hepatic cavernous hemangiomas (hch) were studied with intravenous ct angiography (ivcta). 15 scans were performed during the first 30 s after bolus (6 ml/s) injection of 50 ml iodinated contrast material. thereafter 2 scans/min were obtained up to 30 min. three criteria were utilized at ivcta to make a specific diagnosis of hch: (1) detection of an intense mural nodular enhancement in the arterial phase. the density in the nodular region(s) should have a dens ...20071855518
the antiphospholipid antibody dilemma. 20072168305
'occupational hazards to dental staff'. 20071804266
total hip arthroplasty after fracture of the acetabulum. long-term results.we made a retrospective study of 55 primary total hip arthroplasties in 53 patients with a history of previous acetabular fracture. the mean follow-up was 7.5 years and the average age at fracture was 48.7 years. the incidence of radiographic femoral loosening (29.4%), symptomatic loosening (15.7%), and femoral revision (7.8%) were similar to those previously reported at 10 years for routine arthroplasties by stauffer (1982). on the acetabular side, the incidence of radiographic loosening (52.9% ...20072211750
diagnosis of pancreatic carcinoma by fine needle aspiration cytology and computerized cytomorphometry.eighty-one fine needle aspirations (fnas) of pancreatic masses were performed between 1980 and 1988. histologic or clinical follow-up was available for correlation with 78 aspirates. the fna cytologic diagnosis of pancreatic carcinoma had a sensitivity of 79% and a specificity of 91%. fifteen of the fna specimens were examined with the zeiss ibas image analysis system to determine nuclear area, form (shape), diameter, density and integrated optical density (iod). nuclear area and iod correlated ...20072669782
serological cross-reactivity of environmental isolates of enterobacter, escherichia, stenotrophomonas, and aerococcus with shigella spp.-specific antisera.using protocols designed for the isolation of shigella from environmental freshwater samples from different regions of bangladesh, 11 bacterial strains giving rise to shigella-like colonies on selective agar plates and showing serological cross-reaction with shigella-specific antisera were isolated. phylogenetic analyses revealed that three of the isolates were most closely related to escherichia coli, four to enterobacter sp., two to stenotrophomonas, and two isolates belonged to the gram-posit ...200717171463
vaccine potential for inactivated shigellae.we used human monocyte-derived dendritic cells (dc) and balb/c mice as models to establish the immunogenic and protective potential of formalin-inactivated shigella spp. incubation of dc with inactivated or live bacteria induced dc maturation and cytokine release. mice immunized orally or intranasally with killed s. flexneri, s. sonnei, or s. dysenteriae developed igg and fecal iga titers to the homologous lps. following respiratory challenge with the live homologous organisms, 80-100% survival ...200717178431
structural and genetic evidence that the escherichia coli o148 o antigen is the precursor of the shigella dysenteriae type 1 o antigen and identification of a glucosyltransferase gene.shigella dysenteriae type 1 is the most virulent serotype of shigella. enterotoxigenic escherichia coli o148 is pathogenic and can cause diarrhoea. the following structure was established for the tetrasaccharide repeating unit of the e. coli o148 o antigen: -->3)-alpha-l-rhap-(1-->3)-alpha-l-rhap-(1-->2)-alpha-d-glcp-(1-->3)-alpha-d-glcpnac-(1-->. this differs from the structure reported earlier for s. dysenteriae type 1 by having a glucose (glc) residue in place of a galactose (gal) residue. th ...200717185542
antibacterial activity and in vitro anti-tumor activity of the extract of the larvae of the housefly (musca domestica).one of the significant characteristics of traditional chinese medicine is the use of insects, other terrestrial arthropods and their products as drugs. in the present work, the extract of housefly (musca domestica) larvae was studied by an agar well diffusion assay and minimum bactericidal concentration (mbc) determination for detection of antimicrobial activity and by mtt assay method to test its in vitro anti-tumor activity. in our studies, the extract inhibited six tested bacterial pathogens ...200717227700
using european travellers as an early alert to detect emerging pathogens in countries with limited laboratory resources.the volume, extent and speed of travel have dramatically increased in the past decades, providing the potential for an infectious disease to spread through the transportation network. by collecting information on the suspected place of infection, existing surveillance systems in industrialized countries may provide timely information for areas of the world without adequate surveillance currently in place. we present the results of a case study using reported cases of shigella dysenteriae serotyp ...200717239228
the catalase reaction of shigella species and its use in rapid screening for epidemic shigella dysenteriae type 1.as epidemic dysentery caused by shigella dysenteriae type 1 is associated with high mortality, early identification of outbreaks is important. since s. dysenteriae type 1 differs from most of the enterobacteriaceae in that it does not produce catalase, a test for catalase may provide a useful screening method. the ability of a catalase test to provide rapid identification of s. dysenteriae type 1 has now been assessed, using isolates of this pathogen from five continents, shigella of other speci ...200717244412
heme inhibits the dna binding properties of the cytoplasmic heme binding protein of shigella dysenteriae (shus).heme uptake and utilization by pathogenic bacteria are critical for virulence and disease, since heme and heme proteins are a major source of iron within the host. although the role of outer membrane heme receptors in this process has been extensively characterized at the genetic and biochemical level, the role of the cytoplasmic heme binding proteins is not yet clear. the shigella dysenteriae cytoplasmic heme binding protein, shus, has previously been shown to promote utilization of heme as an ...200717323920
antimicrobial activity of bacillus subtilis and bacillus pumilus during the fermentation of african locust bean (parkia biglobosa) for soumbala production.to examine predominant isolates of bacillus subtilis and b. pumilus isolated from soumbala for their antimicrobial activity against indicator microorganisms as micrococcus luteus, staphyloccocus aureus, bacillus cereus, enterococus facium, listeria monocytogenes, escherichia coli, salmonella typhimurium, shigella dysenteriae, yersinia enterocolitica, aspergillus ochraceus and penicillium roqueforti.200717381739
characterization of the outer membrane receptor shua from the heme uptake system of shigella dysenteriae. substrate specificity and identification of the heme protein ligands.shigella dysenteriae, like many bacterial pathogens, has evolved outer membrane receptor-mediated pathways for the uptake and utilization of heme as an iron source. as a first step toward understanding the mechanism of heme uptake we have undertaken a site-directed mutagenesis, spectroscopic, and kinetic analysis of the outer membrane receptor shua of s. dysenteriae. purification of the outer membrane receptor gave a single band of molecular mass 73 kda on sds-page. initial spectroscopic analysi ...200717387178
detecting low concentrations of shigella sonnei in environmental water samples by pcr.outbreaks of shigella sonnei associated with contaminated water have been reported and methods for the simultaneous detection of shigellae and enteroinvasive escherichia coli in water samples have been developed with detection limits of 10(1)-10(2) cfu ml(-1) of water. because 10(1)-10(2)shigellae can cause disease, a more sensitive detection method as an addition to the existing methods for detection of shigella sonnei in water samples is reported here. initially, 33 shigella sonnei and 72 non- ...200717391373
structural relation of the antigenic polysaccharides of escherichia coli o40, shigella dysenteriae type 9, and e. coli k47.o-polysaccharides were isolated from the lipopolysaccharides of escherichia coli o40 and shigella dysenteriae type 9 and studied by chemical analyses along with (1)h and (13)c nmr spectroscopy. the following new structure of the o-polysaccharide of e. coli o40 was established: -->2)-beta-d-galp-(1-->4)-beta-d-manp-(1-->4)-alpha-d-galp-(1-->3)-beta-d-glcpnac-(1--> theo-polysaccharide structure of s. dysenteriae type 9 established earlier was revised and found to be identical to the reported struc ...200717395169
altered expression of muc2 and muc5ac in response to shigella infection, an in vivo study.infection of mucosal epithelial cells by shigella species leads to an intense and acute inflammatory bowel disease that is characterized by watery diarrhea and purulent discharge. mucin production is a common defense mechanism to protect the underlying mucosa against pathogens. the molecular mechanism(s) underlying mucin induction is unknown in shigellosis. in this study, we have evaluated the relationship between shigella infection, the expression of muc2 and muc5ac and the participation of sig ...200717395379
ryhb, an iron-responsive small rna molecule, regulates shigella dysenteriae virulence.regulation of bacterial gene expression by small rna (srna) molecules is an increasingly recognized phenomenon but one that is not yet fully understood. we show that the srna ryhb suppresses several virulence-associated phenotypes of shigella dysenteriae, a causative agent of bacillary dysentery in humans. the virulence genes repressed by s. dysenteriae ryhb include those encoding the type iii secretion apparatus, its secreted effectors, and specific chaperones. suppression of shigella virulence ...200717438026
a novel serovar of shigella dysenteriae from patients with diarrhoea in bangladesh.every year, around 3 % of isolates from patients with diarrhoea at dhaka hospital, icddr,b, are identified as shigella-like organisms (slos) based on their activity in biochemical tests. these isolates do not react with any of the current shigella antisera including all existing and provisional serotypes. among these slos, a unique cluster of seven isolates with an identical plasmid profile was found and these isolates were further characterized by phenotypic and genotypic techniques. all were n ...200717446289
porin of shigella dysenteriae activates mouse peritoneal macrophage through toll-like receptors 2 and 6 to induce polarized type i response.porin of shigella dysenteriae type 1 coexpressed toll-like receptor (tlr) 2 and tlr6 on peritoneal cavity (perc) macrophages (mphi) of c57bl/6 mice implicating that both the tlrs are essential as a combinatorial repertoire to recognize the protein. besides tlrs, mrna for myd88 and traf6, and nuclear translocation of nf-kappab were enhanced that indicate their involvement in tandem in the activity of porin. the protein selectively up-regulated cd80 on the activated mphi together with mhc class ii ...200716750567
[means of improving the chemoradiotherapy of small cell cancer of the lung].the report deals with an analysis of the results of treatment of 84 patients suffering small cell lung cancer. four regimens of combination chemotherapy as well as radiation were used. complete plus partial regression rates ranged 18-81% (mean-42%) following chemotherapy and 73-87% after radiation treatment. platidiam-containing schemes proved most effective. prophylactic cranial irradiation was followed by increased survival. a correlation between extent of tumor and effectiveness of treatment ...20082839938
[means of improving the chemoradiotherapy of small cell cancer of the lung].the report deals with an analysis of the results of treatment of 84 patients suffering small cell lung cancer. four regimens of combination chemotherapy as well as radiation were used. complete plus partial regression rates ranged 18-81% (mean-42%) following chemotherapy and 73-87% after radiation treatment. platidiam-containing schemes proved most effective. prophylactic cranial irradiation was followed by increased survival. a correlation between extent of tumor and effectiveness of treatment ...20082839938
[computer-assisted preliminary determination of the refraction of intraocular lenses].seventy-seven cases with posterior chamber lenses were examined after suture removal. the residual astigmatism measured 1.27 d. the remaining refraction (spheric equivalent) was 1.6 d. the relation in size of the two corresponding retina images (isoiconia) was taken into consideration by prior determination of lens power based on computerized measurement. the computer program was developed by lepper (bonn). the method of lens power calculation is described.20083755189
[diagnostic value of cranial computerized tomography in pediatric practice].a total of 100 clinical records have been studied to as certain the diagnostic use of brain ct. most frequent diagnoses are: hypoxic-ischemic encephalopaty, seizure disorders, psychomotor delay, head enlargement and trauma. the first years of life was the period with a higher frequency of applications and the greater percentage of pathologic results (75-80 por 100) was found among intracranial hypertension, fetal distress, macrocephaly or infections of central nervous system and their sequelae. ...20082629553
prothrombin requires two sequential metal-dependent conformational transitions to bind phospholipid. conformation-specific antibodies directed against the phospholipid-binding site on prothrombin.prothrombin is a gamma-carboxyglutamic acid-containing protein that binds to phospholipid vesicles in the presence of calcium ions after undergoing a metal ion-induced conformational transition. to integrate recent data into a scheme that is compatible with our knowledge of prothrombin-metal interaction, we have proposed a new model of prothrombin structure. in this model prothrombin undergoes two metal-dependent conformational transitions: pt----pt'----pt*. the first transition is not cation-sp ...20082429962
[surgical scoliosis therapy using vds and harrington instrumentation (ventral derotation spondylodesis)].the vds-method in accordance to zielke and the harrington-distraction-spondylodesis are proved as methods for correction of scoliosis lasting for decades, which late results are world-wide analysed and published. the both diametrical corrective principles and the fundamental different operative techniques settle their different indications, which doubtless depend on the experience of the surgeon. both methods complete each other in operative spectrum of the spinal surgeon. a careful study of the ...20082248610
melanin concentrating hormone (mch): structure-function aspects of its melanocyte stimulating hormone-like (msh-like) activity.melanin concentrating hormone (mch) is a heptadecapeptide, asp-thr-met-arg-cys-met-val-gly-arg-val-tyr-arg-pro-cys-trp-glu-val, synthesized in the brain and secreted from the pars nervosa of teleost fish. this hormone stimulates melanosome (melanin granule) aggregation within integumental melanocytes of fishes but, in contrast, stimulates melanosome dispersion within tetrapod (frog and lizard) melanocytes. we determined the message sequence of the primary structure of mch which is responsible fo ...20082587419
a model for evaluation of gas exchange: mouth to mouth ventilation of infants by emergency medical technicians.we describe a model for evaluating techniques of infant ventilation during resuscitation. the utility of the model is illustrated by testing performance of emergency medical technicians in mouth to mouth ventilation of a model 4 kg infant. ventilation was generally adequate with mean (+/- s.d.) frequency 22 +/- 9 breaths per minute and tidal volume 40 +/- 13 ml. gas delivered to the model consisted of pico2 7 +/- 6 mmhg and fio2 0.20 +/- 0.007. assuming normal metabolic rate and respiratory dead ...20082174185
ifn-treated neuroblastoma cell lines remain resistant to t cell-mediated allo-killing, and susceptible to non-mhc-restricted cytotoxicity.neuroblastoma cell lines can have very low mhc ag expression. the cell lines are insensitive to allo-killing by primed ctl, but are sensitive to non-mhc-restricted cytotoxicity. ifn-gamma increased class i expression, but the cells remained insensitive to ctl. susceptibility to nonrestricted effectors was preserved. class i+ glioma cell lines behaved similarly. the ctl resistance was localized to the recognition phase. neuroblastoma lines did not form conjugates with primed t cells, but were lys ...20082459235
ct of intra-abdominal fluid collections.a wide variety of fluids including ascites, blood, pus, urine, bile, lymph, chyle, mucin, and cerebrospinal fluid can accumulate within the peritoneal and pelvic cavities. the location, appearance, and distribution of this fluid is governed by a number of anatomic and gravitational factors that when carefully analyzed, can help to reveal their source and etiology. computed tomography is ideally suited to document the presence of abdominal and pelvic fluid collections; display their location and ...20082657846
[a germ cell tumor of an ectopic testis in polyorchidism]. 20082547104
cadmium and lead accumulation by goldfish exposed to aqueous refuse incinerator fly ash leachate. 20082597788
[foreign bodies of the digestive tract]. 20082259119
[meta-analysis. a systematic approach to the synthesis of knowledge]. 20082620806
[meta-analysis. a systematic approach to the synthesis of knowledge]. 20082620806
[clinical-experimental examinations on phonetic function in edentulous patients and after insertion of total prostheses measured by the s-sound realization].lose of teeth and therapy by prosthesis interfering with the oral sectors that are necessary for s-sound articulation have an important influence on s-sound realization. hitherto, the resulting s-sound changes used to be evaluated only from a subjective point of view. the application of a complex measuring technique for s-sound analysis permitted to determine the influence of the upper and lower jaw plates on s-sound formation by experiments. in another series of experiments, it was shown that e ...20082640388
[use of the polymorphism of flanking dna probes with respect to the xq-27 region in a great fragile x pedigree].we analyzed the segregation between fragile site xq 27 and flanking polymorphic probes (52a, for factor 9, 4d-8, for factor 8, st14 and mn12) in a great xq-fra pedigree. on the basis of these data, the use of these probes is discussed in view of its value as a diagnostic tool.20082740689
altered queuine modification of transfer rna involved in the in vitro transformation of chinese hamster embryo cells.altered queuine modification of trna has been correlated to neoplastic transformation, but no direct cause and effect relationship has been defined. in the present study, a potential role for this alteration has been assigned. the trna in normal chinese hamster embryo cells is significantly more queuine modified than the trna in their transformed chinese hamster embryo counterparts, even though the specific activity of the queuine modification enzyme is much lower in chinese hamster embryo cells ...20082582452
regulation of cell proliferation and differentiation by interferons. 20082581554
intraspinal opioids: implications for monitoring. monitoring in the intensive care unit is essential.patients receiving intraspinal opiates should be monitored in the intensive care unit for at least 24 hours to prevent potentially lethal outcomes. these include respiratory depression caused by sequestration of the morphine in the cerebrospinal fluid and migration of epidural catheters in the subarachnoid or intravascular space. at this time, most hospitals are not equipped or staffed adequately to guarantee the safety of these patients outside the intensive care unit.20082570133
[differential indications, surgical technics and follow-up treatment concepts of elbow joint surgery in polyarthritis]. 20082576707
the effect of an aversive event on the return of fear.an experiment on the return-of-fear (rof) was carried out on 40 snake- or spider-phobic subjects in order to determine whether an arousing event that occurs shortly before retest influences the magnitude of the rof. additionally, we attempted to produce a reinstatement of fear by introducing an unrelated aversive event (shock) after the fear had been reduced. the arousal manipulation successfully increased subjective arousal but not heartrate responsiveness. the increases in subjective arousal w ...20082573336
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