Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| rapid method for detection, identification, and susceptibility testing of enteric pathogens. | three hundred and seven colonies believed to be enteric pathogens were selected from primary plates of macconkey, xylose desoxycholate, or salmonella-shigella agar for inoculation to lactose-sucrose broth, urea-41 motility medium, modified andrade glucose broth with inverted durham tube, pregrowth broth, triple sugar iron agar, lysine iron agar (lia), and christensen urea agar. the rapid screen consisted of interpreting the lactose-sucrose, urea-41 motility, and modified andrade glucose broth ga ... | 1983 | 6338037 |
| shigellosis with bacteremia: a report of two cases and a review of the literature. | it is widely believed by both physicians and microbiologists that bacteremia is a rare event in shigellosis. we report two cases of shigellosis with positive blood cultures and review 68 cases reported in the literature between 1963 and 1981. we suggest that detection of bacteremia in shigellosis may be facilitated by a greater awareness of the following findings. 1. eighty-seven percent of all cases of shigellosis with bacteremia reported in the literature during the 18-year period reviewed occ ... | 1983 | 6340078 |
| purification and characterization of a shigella dysenteriae 1-like toxin produced by escherichia coli. | a toxin from an enteropathogenic strain of escherichia coli (e. coli h30) was purified to apparent homogeneity from cell lysates. the steps used to isolate the e. coli h30 toxin included french pressure-cell disruption of bacteria grown in iron-depleted media. affi-gel blue chromatography, chromatofocusing, and anti-shiga toxin affinity chromatography. the mobilities of the subunits of radioiodinated e. coli h30 toxin and shiga toxin observed after the two toxins were subjected to sodium dodecyl ... | 1983 | 6341244 |
| shigella infections in the united states, 1974-1980. | during the seven-year period 1974-1980, 93,516 shigella isolates from humans were reported to the centers for disease control, atlanta, through a nationwide surveillance system. over the past 30 years, the reported incidence of shigellosis has been declining in contrast to that reported for salmonellosis. shigella sonnei (group d) now accounts for approximately 70% of the shigella isolates reported, since most of the decline has been due to the increasingly less frequent infections caused by shi ... | 1983 | 6341480 |
| [epidemiology of bacillary dysentery in algeria. i. the epidemiological aspects of dysentery in algeria]. | bacterial dysentery plays an important role among infectious diseases in algeria. a tendency to the growth of the morbidity rate has been observed. dysentery is irregularly spread in different zones of the country. the highest morbidity rate is registered in the sahara zone and in the eastern part of the country. the morbidity rate among the urban population is higher than among the rural population. s. flexneri prevail in the etiological structure of dysentery infection. of all age groups, the ... | 1983 | 6342313 |
| leads from the mmwr. shigella dysenteriae type 2 outbreak in us naval hospital. | 1983 | 6343642 | |
| multiple enteric pathogens in travelers from endemic areas: case report. | 1983 | 6344046 | |
| [eternal return... the reappearance of shiga bacillus in central africa]. | 1983 | 6344812 | |
| outbreak of shigella dysentery in eastern zaire, 1980-1982. | 1983 | 6344814 | |
| isolation and characterization of monoclonal antibodies to shiga toxin. | hybridoma cell lines which produce monoclonal antibodies to shiga toxin from shigella dysenteriae 1 were prepared. the monoclonal antibodies were all of the immunoglobulin g1 isotype and differed in their ability to neutralize cytotoxicity and to bind to shiga toxin in a solid-phase radioimmunoassay. when used for immunoblot analysis, these antibodies were able to identify specifically both nicked and unnicked shiga toxin in crude lysates of s. dysenteriae. | 1983 | 6345399 |
| [new directions in the study of immunity in dysentery]. | 1983 | 6346759 | |
| [causes of sequential changes in the etiological structure of dysentery and the other epidemiologic patterns of this infection]. | 1983 | 6349187 | |
| trimethoprim-sulphamethoxazole-resistant shigella dysenteriae serotype 1 (shiga's bacillus) in gimira, south-west ethiopia. | 1983 | 6354711 | |
| pathogenesis of shigella diarrhea. viii. evidence for a translocation step in the cytotoxic action of shiga toxin. | shigella toxin interacts with hela cells by binding to a glycoprotein cell surface receptor and, after a lag period, inactivates the 60s ribosomal subunit and inhibits protein synthesis. the lag period may be due to energy-dependent translocation of the toxin to the interior of the cells. this step was investigated with agents known to inhibit glucose metabolism, mitochondrial energy production, or macromolecular synthesis and with drugs that alter the cytoskeletal system or lysosomes. metabolic ... | 1983 | 6355319 |
| [topology of the structural proteins of long tail fibers of phages t4d, ddvih+ and ddvih]. | topology of the products of the genes 34, 35, 36 and 37 of the bacteriophage t4d long tail fibers were determined with the aid of monospecific antibodies. the antibodies against gene product 34 were the only to interact with the proximal part of long tail fibers, but the distal part bound the antibodies against 35, 36 and 37. product of the gene 35 is located at the joint-site with the distal part and binds the distance not more than 75 a long. gene product 36 is located between these of 35 and ... | 1983 | 6355821 |
| [trends in the spread of boyd 4 dysentery and the characteristics of its clinical picture and of the biology of its causative agent]. | the data on the proportion of s. boydii 4 in the general structure of the causative agents of dysentery, as well as in the intraspecific structure of s. boydii, in some areas of the ussr in 1977-1981 are presented. s. boydii strains 4, circulating in one of the areas where their proportion considerably increased in 1980-1981, corresponded to their taxonomic position in their biological properties, while forming a single biochemical variant according to the character of the fermentation of sorbit ... | 1983 | 6356722 |
| molecular comparison of virulence plasmids in shigella and enteroinvasive escherichia coli. | virulent isolates of shigella dysenteriae and shigella boydii harboured a 140 mdal plasmid which was either absent or deleted in spontaneously avirulent strains. together with previous data concerning s. sonnei, s. flexneri and enteroinvasive escherichia coli, the present results established the general role of extrachromosomal elements in the virulence of such enteroinvasive species. among different species, these virulence plasmids showed unrelated endonuclease cleavage patterns, whereas hybri ... | 1983 | 6357023 |
| entry of shigella dysenteriae toxin into hela cells. | the rate of shigella toxin entry into the cytosol of hela s3 cells was estimated from the toxin-induced reduction in protein synthesis. whereas high toxin concentrations strongly reduced protein synthesis within 30 min, lower concentrations required longer times. the major part of the cell-bound toxin entered only after several hours. toxin entered cells after incubation at 25 degrees c but not at 20 degrees c, although toxin binding was the same at the two temperatures. increasing the kcl conce ... | 1983 | 6358031 |
| temperature-dependent expression of virulence genes in shigella species. | the pathogenicity of shigella spp. involves the ability of the bacteria to penetrate and replicate within the epithelial cells of the large intestine. model systems for examining the virulence of shigellae employ henle intestinal epithelial cells in tissue culture and an in vivo assay for virulence in guinea pig eyes (sereny test). using these systems, we studied the genetic and physiological bases for the ability of shigellae to invade epithelial cells. we found that expression of virulence in ... | 1984 | 6360895 |
| a small plasmid in shigella dysenteriae 1 specifies one or more functions essential for o antigen production and bacterial virulence. | the role of plasmids in the virulence of shigella dysenteriae 1 w30864, which contains at least five species, was investigated. by means of a standard plasmid-curing procedure, that is, bacterial cultivation at an elevated temperature, five virulence-deficient derivatives were obtained. one of these lacked a small, 6-megadalton plasmid, designated phw400, exhibited reduced invasiveness for hela cells, and failed to produce the somatic antigen. transposon tagging of the phw400 plasmid to produce ... | 1984 | 6360905 |
| [diagnostic test system for the quantitative determination of shigella antigens in patient blood by an immunoenzyme analytical method]. | the diagnostic test system under trial has been shown to permit the detection of s. sonnei and s. flexneri specific antigens with an accuracy of 10(-3) micrograms. along with high sensitivity, the test system has sufficiently high specificity. statistically significant differences in the occurrence of specific dysentery antigens and their levels in the blood of dysentery patients and healthy persons have been revealed. | 1983 | 6362300 |
| studies of enterotoxin of shigella dysenteriae type 1. i. effect of aeration on the production of enterotoxin and suitability of laboratory animals for its biological assay. | shigella dysenteriae type 1 was used to study the influence of aeration on enterotoxin production and the suitability of laboratory animals for the toxin assay. the enterotoxin produced under aerobic shake culture conditions gave a gut dilatory response of 1.436 but that obtained in aerobic and anaerobic stationary cultures exerted no activity. the toxin fractionated on sephadex g-150 column was more effective than that obtained by ammonium sulphate precipitation. only sephadex column fraction i ... | 1983 | 6362316 |
| purified shigella enterotoxin does not alter intestinal motility. | a purified shigella enterotoxin (pst) and a cell-free lysate with pst removed (cfl-pst) from the whole-cell lysate of shigella dysenteriae 60 r were used to study their effect on the myoelectric activity and mucosal integrity of rabbit ileal segments. we have previously defined two myoelectric patterns: the migrating action potential complex and repetitive bursts of action potentials that occur in response to certain bacteria and their enterotoxins. the in vivo model consisted of isolated ileal ... | 1984 | 6363286 |
| shiga toxin: no smoking gun. | 1984 | 6364352 | |
| r plasmids conferring multiple drug resistance from shigella isolated in korea. | the majority (85%) of shigella isolated in 1980 and 1981 in korea were shigella flexneri, the others were sh. sonnei (14%) with only a small number of sh. dysenteriae. only 14 of the 459 strains of shigella isolated were susceptible to all 12 drugs tested, and 445 were resistant to three or more drugs. strains multiply resistant to the six drugs, chloramphenicol (cm), tetracycline (tc), streptomycin (sm), sulfisomidine (su), ampicillin (ap) and trimethoprim (tp) were most frequently encountered, ... | 1984 | 6368684 |
| shigella dysentery in tripoli, libya. | 1984 | 6371260 | |
| immunochemical studies on a polysaccharide from shigella dysenteriae type 2. | the polysaccharide isolated from shigella dysenteriae type 2, strain nctc 566, on smith degradation and graded hydrolysis yielded three oligosaccharides which were characterised using methylation studies. using homologous rabbit antiserum and the monosaccharides that constituted the polysaccharide and the oligosaccharides isolated from it and the cross-reactions in some type-specific pneumococcal antisera, immunochemical specificities of different sugar groupings in the polysaccharide molecule w ... | 1983 | 6371498 |
| [transfer of h2s plasmid from e. coli to shigella]. | 1983 | 6374333 | |
| [recent advances in the molecular mechanism of action of bacterial toxins, in particular of diphtheria, cholera, coli, botulinum and shigella toxins as well as tetanospasmin and the toxins of staphylococcus aureus]. | great progress was achieved in the clarification of the molecular structure and the mechanism of action of the toxins of pathogenic forms of bacteria. proportions of toxins of corynebacterium diphtheriae and of pseudomonas aeruginosa transfer from the nad and adp-ribose protein to an amino acid of the elongation factor 2. thus the protein synthesis is much inhibited. the cholera toxin and the l-toxin from escherichia coli have a similar structure. they transfer an adp-ribose portion from nad to ... | 1984 | 6375175 |
| dried filter paper for sending dysenteric faeces to the laboratory. a neglected method? | 1984 | 6375592 | |
| circulating antisomatic antibody response in shiga dysentery & igm & igg activities after 2-mercaptoethanol treatment. | 1984 | 6378776 | |
| [arthritis in shigella and salmonella infections]. | 1984 | 6379799 | |
| [shigellosis abroad]. | 1983 | 6379992 | |
| changes in the trend of shigellosis in dhaka: family study on secondary infection, clinical manifestation and sensitivity pattern: 1980. | the incidence of shigellosis and the death rate have increased and the resistance of shigellae to antibiotics has changed in dhaka during our experiences. in 1980, we investigated the secondary infection and case rates, infection to case ratio, duration of illness, excretion of shigellae and antibiotic sensitivity pattern in 100 families with cases of shigellosis, culturing rectal swabs obtained by home visits for a 10-day period. standard methods were used for culture and sensitivity tests. the ... | 1984 | 6380011 |
| effect of bacterial endotoxin on body temperature, plasma zinc and plasma concentrations of the acute-phase protein serum amyloid p component in mice. | bacterial endotoxin and lipid a evoked dose-dependent increases in body temperature and plasma sap concentrations and dose-dependent falls in plasma zinc concentrations in mice. the respective sensitivities of the three variables to lipid a or to whole endotoxin had the relation sap greater than zinc much greater than body temperature; zinc and sap responses were evoked by less than 1 ng lipid a/endotoxin. pretreatment with indomethacin prevented only the temperature response to lipid a. pretrea ... | 1984 | 6380557 |
| small virulence plasmid of shigella dysenteriae 1 strain w30864 encodes a 41,000-dalton protein involved in formation of specific lipopolysaccharide side chains of serotype 1 isolates. | a 6-megadalton plasmid, phw400, of shigella dysenteriae 1 strain w30864 was previously found to specify one or more functions for o-antigen production and bacterial virulence (h. watanabe and k. n. timmis, infect. immun. 43:391-396, 1984). the region of phw401, a tn801-tagged derivative of phw400, responsible for o-antigen production has been localized by gene cloning and tn5 transposon mutagenesis. analysis of lipopolysaccharide isolated from s. dysenteriae 1 bacteria carrying mutant plasmids r ... | 1984 | 6384048 |
| [a patient with a complicated course of shigellosis]. | 1984 | 6384800 | |
| the humoral antibody response to shigella dysenteriae type 1 infection, as determined by elisa. | an enzyme-linked immunosorbent assay (elisa) for determining the class-specific humoral antibody response to the lipopolysaccharide antigen from shigella dysenteriae serotype 1 bacteria has been tested. two or more serum samples from each of 60 persons infected with this organism during a dysentery outbreak in a boarding school for young men near haiphong, viet nam, and single serum samples from 39 healthy vietnamese and from 20 healthy swedes were included in the study. comparison of the titres ... | 1984 | 6386207 |
| usefulness of plasmid profiles for differentiation of shigella isolates in bangladesh. | we studied the plasmid profiles of 136 shigella isolates in bangladesh to determine whether plasmid profiles could be used for differentiation of strains for epidemiological studies. many different plasmid patterns were observed within each species, indicating that many genetically different strains of shigella are responsible for illness in bangladesh. | 1984 | 6386850 |
| expression of lipopolysaccharide o antigen in escherichia coli k-12 hybrids containing plasmid and chromosomal genes from shigella dysenteriae 1. | the requirement for both plasmid and chromosomal genes in the biosynthesis of shigella dysenteriae 1 lipopolysaccharide o antigen was demonstrated in escherichia coli-shigella hybrids. a 6-megadalton s. dysenteriae 1 plasmid, designated pwr23, was phenotypically tagged with the tn3 ampicillin-resistance transposon. the tagged plasmid, designated pwr24, was transferred by transformation or conjugal mobilization to a rough e. coli k-12 recipient. although the resultant hybrids were agglutinated in ... | 1984 | 6389345 |
| epidemic dysentery caused by the shiga bacillus in a southern indian village. | multi-drug resistant shigella dysenteriae type 1 caused an epidemic of dysentery in a village in southern india. the epidemic started as a common source outbreak, through the piped water supply of the village with subsequent person-to-person spread. although the attack rate was high, with nearly 1/2 the children under age 5 being affected, the case fatality rate was only about 1%. of the 248 patients who attended the field clinic, 89.4% passed small mucoid stools mixed with blood, with the ma ... | 1984 | 6389664 |
| [bacillary dysentery 1982]. | 1984 | 6390523 | |
| epidemic shiga bacillus dysentery in central africa. | an outbreak of dysentery began late in 1979 in central africa and spread to involve a major portion of zaire as well as rwanda and burundi. we traveled to a mission hospital in northeast zaire during the epidemic and isolated shigella dysenteriae, type 1, from most of the patients studied. all isolates were resistant to ampicillin, tetracycline, chloramphenicol, sulfathiazole, and streptomycin but sensitive to trimethoprim-sulfamethoxazole. antimicrobial resistance was transferable to escherichi ... | 1984 | 6391226 |
| [molecular structure of the o-specific polysaccharide of shigella dysenteriae type 2]. | 1984 | 6391882 | |
| pathogenesis of shigella diarrhea. ix. simplified high yield purification of shigella toxin and characterization of subunit composition and function by the use of subunit-specific monoclonal and polyclonal antibodies. | a simple purification scheme for shigella cytotoxin was devised, resulting in high yields (approximately 50%) and a 1,300-fold increase in specific activity compared with the initial crude bacterial cell lysate. the purified toxin was enterotoxic in ligated rabbit ileal loops and neurotoxic when injected into the peritoneal cavity of mice. measurement of specific activity of cytotoxin and enterotoxin demonstrated that these two toxicities copurify during the fractionation procedure. on sodium do ... | 1984 | 6392471 |
| [the local epidemic of shigella dysenteriae in wuwei county, gansu province]. | 1984 | 6394140 | |
| shigellemia. | 1984 | 6394497 | |
| [shigella dysenteriae in our environment]. | 1984 | 6394922 | |
| shigellosis: an old disease with new faces. | 1984 | 6397507 | |
| an epidemic of blood and mucus diarrhoea complicated by disseminated intravascular coagulation. | 1984 | 6399690 | |
| hospital-associated outbreak of shigella dysenteriae type 2--maryland. | 1983 | 6405168 | |
| drug resistance in shigella dysenteriae, s flexneri and s boydii in england and wales: increasing incidence of resistance to trimethoprim. | a total of 2753 strains of shigella belonging to subgroups a, b, and c that were isolated from patients in england and wales during the period from 1979 to mid-1983 were studied. of these, 1690 (61%) were from patients recently returned from abroad or in contact with recent travellers, and 760 (45%) of these affected travellers from the indian subcontinent. the number of strains resistant to sulphonamides and streptomycin remained at a high level throughout (average 76% and 72% respectively). re ... | 1984 | 6423079 |
| [clinical and laboratory studies on acute bacterial diarrhea]. | 1984 | 6437636 | |
| the leukemoid reaction in shigellosis. | leukemoid reactions occurred in 136 patients (3.8%) hospitalized with shigellosis in bangladesh. sixty-eight percent of the patients with leukemoid reactions were children less than 4 years old. when compared with patients without leukemoid reactions, the leukemoid reactions were significantly associated with children aged less than 10 years. the most common serotype of shigella in the patients with leukemoid reactions was shigella dysenteriae 1, isolated from 96 patients (71%), whereas the most ... | 1984 | 6695872 |
| production of shigella dysenteriae type 1-like cytotoxin by escherichia coli. | strains of escherichia coli previously implicated or proven to be causes of diarrhea were examined for production of a toxin similar to that of shigella dysenteriae type 1 (shiga). organisms grown in an iron-depleted broth were lysed by pressure disruption followed by ultracentrifugation. saline-dialyzed extracts were tested for cytotoxic effects on hela cells that were neutralizable with antiserum to shiga toxin. among the 13 e. coli strains so analyzed, 11 made a shiga-like cytotoxin in levels ... | 1982 | 6754826 |
| [bacillary dysentery - 1980]. | 1982 | 6755564 | |
| [use of coagglutination for serotyping shigella dysenteriae and shigella boydii]. | the coagglutination test was used to identify shigella boydii and shigella dysenteriae. a trial was carried out with 13 native rabbit antisera to s. boydii and 10 antisera to s. dysenteriae, as well as with coagglutinating reagents prepared from these antisera. the use of coagglutinating reagents was shown to ensure the complete specificity of the results, to prevent the adsorption of diagnostic antisera and to decrease their consumption 50 times. the importance of the coagglutination test for t ... | 1982 | 6760638 |
| bacterial adhesiveness and invasiveness in cell culture monolayer. 2. in vitro invasiveness of 45 strains belonging to the family enterobacteriaceae. | the invasive potential of 45 presumptive enteropathogenic and non-enteropathogenic bacterial strains belonging to the family enterobacteriaceae have been tested using the serény test and hep-2 cell monolayers examined by a combined light optical method. all the presumptive enteropathogenic strains of shigella dysenteriae, s. boydii, s. flexneri, s. sonnei and salmonella typhimurium showed in vitro invasiveness in the hep-2 cell culture test. fourteen presumptive non-enteropathogenic strains of e ... | 1982 | 6762798 |
| isolation of the shigella genus bacteria from the beach sand and water of the bay of gdańsk. | 1982 | 6764618 | |
| [anti-shigella dysenteriae 1 antibodies in the mexican population. their possible relation to the central american outbreaks of 1968-70]. | 1982 | 6765039 | |
| foodborne outbreak of shigellosis caused by an unusual shigella strain. | a family outbreak of foodborne shigellosis caused by an unusual strain of shigella is described. the strain was a mannitol-positive variant of shigella dysenteriae and agglutinated in antiserum prepared against provisional serotype 3341-55. | 1980 | 6768766 |
| comparative studies on the regulation of tryptophan synthesis. | in vitro dna recombination techniques have revolutionized the study of genetic control of biosynthetic pathways. using examples drawn from the pathway of tryptophan synthesis, approaches to the deciphering of regulatory signals and response mechanisms through transposition of dna segments and dna sequence analysis will be presented. after reviewing the known chromosomal arrangements and regulatory patterns of trp genes in the bacterial groups studied so far, and describing the results of transfe ... | 1980 | 6772375 |
| effects of intravenous and aerosol administration of crude shigella toxin to rhesus macaques: preliminary study. | experiments were conducted to determine the dose response, survival time, blood biochemical changes, and cardiohepatic responses to a single iv injection of crude shigella toxin in rhesus macaques (macaca mulatta). circulatory shock, hepatic falure, respiratory depression, dyspnea, convulsions, and coma were observed before death. the survival time was inversely related to the administered toxic dose, which ranged from 20 to 200 microgram/kg of body weight. however, the severity of lesions in th ... | 1980 | 6773452 |
| [features of the infectious process caused by shigellae of different species]. | 1980 | 6778021 | |
| increase in drug resistance among shigella dysenteriae, sh flexneri, and sh boydii. | two thousand three hundred and seventy strains of shigella dysenteriae, sh flexneri, and sh boydii isolated in england and wales from 1974 to 1978 were tested for resistance to 12 antimicrobial drugs. eighty per cent of strains were resistant to one or more drugs, with sulphonamide resistance occurring most frequently. resistance to streptomycin, tetracycline, ampicillin, and chloramphenicol increased during the period, as did the incidence of multiple resistance. most infections due to sh dysen ... | 1981 | 6790085 |
| immune response to parental and rough mutant strains of salmonella minnesota. | specificity of immune response to smooth and rough mutant strains of salmonella minnesota was investigated. immunization of mice with rd and re rough mutants resulted in formation of bactericidal plaque-forming cells directed against the lipopolysaccharide structure of both the mutants and the parental smooth strain. these antibody plaques, however, were not bactericidal for smooth strains of other gram-negative species, i.e., escherichia coli, salmonella typhosa, klebsiella pneumoniae, shigella ... | 1981 | 6793518 |
| behavioural and body temperature effects of meningococcal lipopolysaccharide after intraventricular injection in adult fowls gallus domesticus. | the effects of the meningococcal lipopolysaccharide (lps), given into the ii cerebral ventricle of adult fowls, were studied on behaviour and body temperature. immediately after the administration a marked and dose- dependent behavioural sedation or sleep lasting over 2 hours or more according to the dose was observed. the body temperature effects of lps were biphasic, i.e. and immediate initial phase of hypothermia lasting about 100 min, followed by a longer-lasting fever response. a pretreatme ... | 1981 | 6800001 |
| increase in drug resistance among shigella dysenteriae, sh flexneri, and sh boydii. | 1982 | 6803969 | |
| [antigens of shigella dysenteriae serovars 3-7. iv. immunochemical study of the exotoxin]. | the exotoxins of sh. dysenteriae, serovars 3 and 7, possess antigenic and serological properties and are characterized by the heterogeneous antigenic structure which distinctly differs from the structure of o-antigen in its immunochemical properties. these exotoxins consist of thermolabile and thermostable components. the thermolabile exotoxin fraction is a lethal toxin. the thermostable exotoxin fraction, obtained from the cultures of sh. dysenteriae in the s-form, corresponds to o-antigen and ... | 1982 | 6808792 |
| shigella dysenteriae bacteremia. | 1983 | 6889165 | |
| the cytotoxic activity of shigella toxin. evidence for catalytic inactivation of the 60 s ribosomal subunit. | the cytotoxic test system for shigella shigae toxin was improved and used to study the stability of the toxin to various ph values, temperature, and chemicals. inhibition of protein synthesis is the first demonstrable effect in cells treated with shigella toxin. this inhibition appears to be at the level of peptide chain elongation. an inhibition effect on cell-free protein synthesis is exhibited by toxin pretreated first with trypsin and then with dithiothreitol and 8 m urea or 1% sodium dodecy ... | 1981 | 6894922 |
| interaction of shigella shigae cytotoxin with receptors on sensitive and insensitive cells. | the effect of a cytotoxin isolated from shigella shigae has been tested on different cell lines. hela s3 cells, as well as some other human carcinoma cells, were killed by picomolar to femtomolar concentrations of the pure toxin, whereas certain other human carcinoma cells and a variety of non-epithelial cells from human tissue and from various animal tissues were resistant to-nanomolar concentrations of the toxin. binding studies with 125 i-labelled shigella shigae cytotoxin showed that the sen ... | 1980 | 6895387 |
| in vitro antibacterial activity of fluorinated analogs of chloramphenicol and thiamphenicol. | we evaluated the in vitro antimicrobial activity of sch 24893, sch 25298, and sch 25393, three novel analogs of chloramphenicol and thiamphenicol. all of the analogs had minimal inhibitory concentrations of less than or equal to 10 micrograms/ml for 18 chloramphenicol-thiamphenicol-resistant strains of shigella dysenteriae and 21 strains of resistant salmonella typhi. the analogs were also more active than were chloramphenicol and thiamphenicol against chloramphenicol-resistant enteric bacteria, ... | 1981 | 6957162 |
| fever, antipyretics and serum iron levels in rabbits. | the intracerebroventricular (i.c.v.) injection of bacterial endotoxin (shigella dysenteriae) produces a rise in deep-body temperature in rabbits. the increase in body temperature was partially inhibited by polymyxin b (p), and completely suppressed by ketoprofen (k). the systemic injection of bacterial endotoxins (shigella dysenteriae or salmonella typhosa) elicited an elevation of body temperature in rabbits which was partially blocked by polymyxin b and completely antagonized by ketoprofen. se ... | 1982 | 6983446 |
| plasmids and antibiotic resistance in gram-negative bacteria. | 1980 | 6987459 | |
| prolonged intermittent diarrhea after shiga dysentery: postdysenteric syndrome. | a 42-year-old woman had dysentery caused by the shiga bacillus, shigella dysenteriae type 1, while taking diphenoxylate with atropine during and after her return from a trip to mexico. although she was treated with appropriate antibiotics, she suffered a prolonged and toxic acute course followed by intermittent bouts of diarrhea and abdominal cramping which persisted for two years. the risk of confusing shiga dysentery with ulcerative colitis is illustrated by the presentation, management, and p ... | 1980 | 6987740 |
| shigella toxin inhibition of binding and translation of polyuridylic acid by escherichia coli ribosomes. | shigella toxin inhibits polyuridylic acid-directed polymerization of phenylalanine in ribosome-enzyme systems obtained from escherichia coli or from shigella dysenteriae. the inhibition is the result of toxin acting on ribosomes to prevent polyuridylic acid attachment. | 1980 | 6988411 |
| bacillary dysentery due to multidrug resistant sh. dysenteriae type 1. | 1980 | 6991403 | |
| regulation of tryptophan biosynthesis. | 1980 | 6996563 | |
| [formation of iron-binding metabolites by bacteria of the enterobacteriaceae family]. | the capacity of enterobacteriaceae strains cultivated in a synthetic medium for the biosynthesis of catechols and hydroxamates has been studied. among the strains under study all strains of the genera salmonella (8 strains), escherichia (102 strains), citrobacter (5 strains), enterobacter (2 strains), serratia (1 strain) synthesize catechols. in the genus shigella (128 strains) all sh. flexneri serovars (79 strains) do not synthesize catechols, other representatives of this genus synthesize iron ... | 1980 | 6998212 |
| association of human enteric pathogenicity and mouse lung toxicity of escherichia coli. | mouse lung toxicity of 439 strains (431 escherichia coli, 1 shigella dysenteriae 1, 1 enterobacter cloacae, 5 vibrio sp., 1 klebsiella) was compared to other pathogenicity tests (mouse virulence, enterotoxicity, guinea pig eye test), to serogroup distribution, loss of virulence on storage, origin and haemolytic activity. mouse lethality was highest in serogroup o4 (p < 0.001), o18a,c (p < 0.001); serogroups o6, o20, o75, o115, o147 were next in order. e. coli serogroups o19, o26, o28a,b, o32, o5 ... | 1980 | 6998258 |
| esterase electrophoretic pattern relatedness between shigella species and escherichia coli. | esterases of 57 strains of shigella dysenteriae, sh. flexneri, sh. boydii and sh. sonnei and 26 strains of escherichia coli, including the alkalescens dispar group, were compared by polyacrylamide-agarose gel electrophoresis. six types of esterase bands differing in their ability to hydrolyse synthetic substrates and in their sensitivity to heat and to di-isofluoropropyl phosphate were defined. individual activities and sensitivities of these bands and the apparent molecular weight of the major ... | 1980 | 6999118 |
| randomized trial of high- and low-dose ampicillin therapy for treatment of severe dysentery due to shigella dysenteriae type 1. | to establish optimal therapy for severe dysentery due to shigella dysenteriae type 1 and shigella flexneri, we compared in a prospective randomized trial two oral ampicillin doses (50 and 150 mg/kg per day) in 57 children and 39 adults in dacca, bangladesh. clinical failure did not occur in either group, indicating that conventional doses need not be increased even in severe disease. among children 3 years of age or under, bacteriological relapses tended to be more frequent in the low-dose group ... | 1980 | 6999981 |
| cytotoxicity of shigella dysenteriae 1 for cultured mammalian cells. | the cytotoxicity of an invasive toxigenic wild-type strain of shigella dysenteriae 1 (3818t) was compared with that of noninvasive, toxigenic strain 38180 and hypotoxigenic strain 725. cytolysis of hela or henle 407 cells exposed to these strains was measured by release of (3h) uridine from prelabeled monolayers. hela cells exposed to noninvasive, toxigenic strain 38180, or to partially purified shiga toxin were lysed only after a latent period of more than 8 hr. during this period, protein synt ... | 1980 | 7001884 |
| shigella dysenteriae i enterotoxin: proposed role in pathogenesis of shigellosis. | bacterial strains of shigella dysenteriae i (3818-t and 3818-o) and shigella enterotoxin altered myoelectric activity of the small intestine in new zealand white rabbits. these agents were compared with activity caused by sterile culture broth or sterile saline. the altered myoelectric activity was characterized by two distinct complexes: repetitive bursts of action potentials (rbap), characteristic of invasive strains of bacteria, and the migrating action potential complex (mapc), characteristi ... | 1980 | 7001925 |
| characterization of shigella dysenteriae 1 (shiga) toxin purified by anti-shiga toxin affinity chromatography. | shigella dysenteriae 1 (shiga) toxin was purified from whole-cell lysates by antitoxin affinity column chromatography, radioiodination, and sephacryl s-200 gel filtration of 125i-labeled affinity column eluates. two chromatographic peaks were observed. the percentage of radioactivity in peak i samples immunoprecipitated with antitoxin ranged from 95 to 100%. a pool of samples from this first peak contained over 90% of the hela-cell-cytotoxic units applied to the column and was enterotoxic for ra ... | 1980 | 7002787 |
| isolation and characterization of minicell-producing mutants of shigella spp. | minicells are small, anucleate cells resulting from aberrant cell divisions at the polar ends of bacilli. we have isolated minicell-producing mutant strains of shigella flexneri 2a (mc-i) and shigella dysenteriae 1 (mc-v) after mutagenesis with n-methyl-n'-nitro-n-nitrosoguanidine. microscopically, broth cultures of mc-i and mc-v were found to contain free minicells, normal cells, and filamentous cells with polar, attached minicells. both strains retained their ability to provoke keratoconjuncti ... | 1980 | 7002790 |
| contrasting epidemiology of shigellae dysenteriae and shigellae flexneri, dacca. | 1980 | 7003832 | |
| [characteristics and function of enterotoxins of gram negative bacteria (author's transl)]. | several factors are important for the pathogenesis of bacterial intestinal disease: 1. colonisation of the intestine; 2. exo(= entero)toxin production without penetration into the intestinal wall; 3. invasion of the intestinal wall with or without (?) simultaneous formation of enterotoxin. these factors are of different importance for the various infective agents. whereas cholera is exclusively caused by an enterotoxin, this is of minor importance for the pathogenesis of shigella dysentery. the ... | 1980 | 7005079 |
| survival of shigellae in soil. | the survival of four shigella strains in soil (sh. sonnei, sh. boydii, sh. flexneri and sh. dysenteriae) was studied under various conditions. their survival period was tested in two different types of sterile soil at 18-20 degrees c and in one type of soil at 4 and 37 degrees c. this latter type of soil, after enrichment with casaminoacids or (nh4)2hpo4 was also used for testing again the survival of the strains at 18-20 degrees c. though the initial number of the inoculated microorganisms was ... | 1980 | 7006270 |
| relative potencies of four reference endotoxin standards as measured by the limulus amoebocyte lysate and usp rabbit pyrogen tests. | four commonly used reference endotoxin standards, escherichia coli o113:h10:k0, e. coli o55:b5, salmonella abortusequi, and shigella dysenteriae were compared by the usp rabbit pyrogen and the limulus amoebocyte lysate tests. by the rabbit pyrogen test, s. abortus equi was identified as the most potent endotoxin, followed closely by e. coli o113:h10:k0 and e. coli o55:b5. | 1980 | 7006505 |
| resistance to trimethoprim and other antibiotics in shigellae isolated in the province of ontario. | antimicrobial susceptibility determinations for three commonly used and seven seldom used antibacterial drugs against 482 strains of shigellae isolated in ontario during 1977 and 1978 were carried out. resistance to the first-line treatment drugs, ampicillin and tetracycline, occurred in 20.5 and 39.2% of strains, respectively. the emergence of trimethoprim-sulfamethoxazole resistance was noted for the first time in 3.2% of the strains in 1978. all strains retained their susceptibility to gentam ... | 1980 | 7006767 |
| nucleotide sequences of the trpg regions of escherichia coli, shigella dysenteriae, salmonella typhimurium and serratia marcescens. | 1980 | 7007652 | |
| protein synthesis in hela or henle 407 cells infected with shigella dysenteriae 1, shigella flexneri 2a, or salmonella typhimurium w118. | the incorporation of [14c]leucine into protein was studied in two mammalian cell lines which had been infected with strains of shigella dysenteriae 1, shigella flexneri 2a, or salmonella typhimurium w118. these cell lines differed in susceptibility to the effects of exogenously applied shiga cytotoxin. all invasive shigella strains (which synthesize this toxin to a greater or lesser degree) were found to inhibit protein synthesis in both cell lines with equal efficiency. leucine accumulation con ... | 1981 | 7012026 |
| quantitative microtiter cytotoxicity assay for shigella toxin. | the cytotoxic activity of shigella dysenteriae 1 was assayed by exposing hela cells in microtiter cultures to dilutions of toxin. exposure to toxin caused either failure of cells in suspension to attach or detachment of cells from established monolayers. estimates of toxin potency were made by staining residual cells with crystal violet and visually inspecting the stained plates. quantitation of the cytotoxic effect was made possible by eluting and spectrophotometrically measuring the stain. the ... | 1980 | 7012172 |
| enterotoxigenic bacteria in food and water from an ethiopian community. | food and water samples from an ethiopian community were screened for the presence of enterotoxin-producing bacteria. using the chinese hamster ovary cell assay, 40 of 213 isolates (18.8%) produced heat-labile (lt) enterotoxin. these lt-producing isolates comprised 33 of 177 (18.6%) strains from 24 of 68 food samples (35.3%) and 7 of 36 (19.4%) isolates of 4 of 17 water samples (23.5%). one lt-producing strain each of salmonella emek and of shigella dysenteriae was found. three pseudomonads, all ... | 1981 | 7016032 |
| salmonella and shigella surveillance in hungary, 1972-1976. ii. shigella surveillance. | shigellae were isolated from 32 399 persons in the bacteriological laboratories of the public health stations between 1972-1976. the number of isolations ranged between 5325 and 8237 yearly. as s. dysenteriae and s. boydii serotypes occurred only in about 1.5% of all isolations, the epidemiological situation was determined by the incidence of s. flexneri and s. sonnei. except in 1973, s. sonnei constituted the majority in every year. s. sonnei predominance, observed first in the western regions ... | 1981 | 7020354 |
| subunit structure of shigella cytotoxin. | shigella cytotoxin was purified from the culture medium of shigella shigae 60 r and from the bacterial pellet by an extensive purification scheme involving chromatography on cibachron blue f3ga, chitin, and deae-cellulose columns, sucrose gradient centrifugation, and finally polyacrylamide gel electrophoresis under nondenaturing conditions. in sodium dodecyl sulfate polyacrylamide gels, purified toxin showed two bands, a heavy one with the molecular weight of 30,500 (a chain), and a broad band m ... | 1981 | 7021550 |
| [serologic affinity and specificity of action of pseudotuberculosis and coli-dysentery phages]. | the study of serological properties, specificity and the range of action has revealed affinity between y. pseudotuberculosis phages (pst, 3m, kotlyarova, 2344, 2391), some coliphages (t2, t3, t4) and sh. dysenteriae phage (dd iv). the existence of serovar iii of y. pseudotuberculosis phages has been established; to this serovar phage pst belongs. newly isolated 2344 and 2391 belong to serovar i. the problem of the existence of y. pseudotuberculosis phages as an independent group is discussed. | 1981 | 7023155 |
| [diagnosis of grigor'ev-shiga dysentery]. | the data on the relative significance and composition of sh. dysenteriae serovars isolated in some regions of the ussr in 1975-1979 are presented. special tests revealed that endo agar was the best medium, while ploskirev's agar was the worst medium for growing sh. dysenteriae 1 (the museum strain and the newly isolated strain from bangladesh). the testing of agar with eosine-methylene blue yielded moderate results in respect of the growth of the museum strain; as to the growth of the strain fro ... | 1981 | 7025526 |