Publications

comparative studies on the accessibility and functional importance of tyrosine residues in cytochrome p-450 isozymes.	cytochromes p-450 lm2 and p-450 lm4 from rabbit liver microsomes were chemically modified with tetranitromethane. nitration of two tyrosine residues of both isozymes inhibits the benzphetamine n-demethylase activity of p-450 lm2 as well as the p-nitrophenetole o-deethylase activity of p-450 lm4 by about 80%. for identification of the modified tyrosine residues the inactivated enzymes were digested with trypsin, and the labeled peptides were separated by hplc. sequencing of the 3-nitrotyrosine-co ...	1988	3202847
rapid preparation of samples for compositional sugar analysis of the "degraded polysaccharide" fraction of lipopolysaccharides from vibrio cholerae.	a simple and rapid method was devised for direct isolation and fractionation of the "degraded polysaccharide" (dps) fraction of o-antigenic (or endotoxic) lipopolysaccharides (lps) directly from heat-killed vibrio cholerae (o1 and non-o1) cells without separating the lps. neither phenol-water extraction nor ultracentrifuge is needed in this method. v. cholerae nih 41 was used as standard. the cells (3-5 g wet weight) were heated in 5% acetic acid at 100 c for 1.5 hr. the acetic acid extract obta ...	1988	3210996
effects of iron and temperature on shiga-like toxin i production by escherichia coli.	iron is known to depress shiga toxin production by shigella dysenteriae 1, and temperature has been shown to regulate several genes required for shigella invasiveness. in this study, the influence of iron and temperature on regulation of a highly related toxin, shiga-like toxin i (slt-i) of enterohemorrhagic escherichia coli, was examined in strains lysogenic for the toxin-converting coliphage 933j and in strains carrying the cloned slt-i genes on a high-copy-number plasmid vector. for compariso ...	1988	3121508
characterization of the protein expressed in escherichia coli by a recombinant plasmid containing the bacillus megaterium cytochrome p-450bm-3 gene.	in two previous reports (narhi lo, fulco aj, j. biol. chem. 261: 7160-7169, 1986; ibid., 262: 6683-6690, 1987) we described the characterization of a catalytically self-sufficient 119,000-dalton p-450 cytochrome that was induced by barbiturates in bacillus megaterium. in the presence of nadph and o2, this polypeptide (cytochrome p-450bm-3) catalyzed the hydroxylation of long-chain fatty acids without the aid of any other protein. the gene encoding this unique monooxygenase was cloned into escher ...	1988	3131661
shigella dysenteriae type 1 in tourists to cancun, mexico.		1988	3135475
effects of dietary-fish-oil feeding on muscle growth and damage in the rat.	1. giving diets containing 100 g fully-refined, non-hydrogenated fish oil/kg to rats caused substantial modification of skeletal-muscle-membrane fatty acid composition compared with control animals fed on an equivalent diet containing 100 g maize oil/kg. 2. total muscle arachidonic acid (20:4 omega 6) was reduced from 138 (sd 25) mg/g total fatty acids to 15 (sd 2) mg/g and phospholipid arachidonic acid content showed equivalent changes. 3. reduction in muscle arachidonic acid content had no inf ...	1988	3143398
fatal shigellosis in a chimpanzee (pan troglodytes) in the jos zoo, nigeria.	shigellosis due to shigella dysenteriae was diagnosed in an adult male captive chimpanzee (pan troglodytes) which died suddenly after a brief recovery from illness lasting at least 3 wk. confirmatory diagnosis was based on postmortem examination and cultural isolation of shigella dysenteriae from the intestine, liver, lung, spleen and heart blood.	1988	3280840
[hoof diseases as a result of stall floor defects].	the pig's hoof is characterized by sudden changes between hard and soft horn and a high growth rate of the horn wall. these facts contribute to the high incidence of hoof alterations, which is seen in various inadequate floors. hoof alterations are only painful in cases, where the corium is irritated. some hoof alterations are described, the question of poor footing is discussed. the author provides recommendations regarding the recognition of inadequate barn floors and their improvement. stable ...	1988	3368898
the effect of chlorhexidine and benzydamine mouthwashes on mucositis induced by therapeutic irradiation.	a variety of mouthwashes are frequently used in the management of irradiation-induced mucositis. benzydamine has recently been introduced for alleviating this condition. its efficacy as a mouthwash was compared with chlorhexidine in two groups of patients receiving radiotherapy for oral carcinoma. mucositis and pain were recorded over a 6 week period and oral carriage of candida species, coliforms and staphylococcus aureus was assessed using an oral rinse technique. there was no significant diff ...	1988	3293887
interaction of horse-spleen ferritin with aluminium citrate.	horse-spleen ferritin was found to bind al systematically following gel filtration in buffered al citrate 30 microm, and up to molar ratio 98 when incubated at 37 degrees c with al citrate, buffered to ph 7.4. pre-incubation with 3 concentrations of neutral sodium phosphate (0.1, 1.0, 10.0 mm) had no significant effect on binding. apotransferrin interaction with the al-ferritin complex to release fe but not al. protein-digestion and edta washing procedures showed that the al was firmly bound to ...	1988	3147825
lactic acidosis and recovery of neuronal function following cerebral hypoxia in vitro.	the rat hippocampal slice preparation was used to study the combined effects of hypoxia and lactic acidosis on neuronal function. control slices were exposed to a standard hypoxic insult while being perfused with normal artificial cerebrospinal fluid (acsf). experimental slices were perfused with acsf containing 1.0, 2.0, 10.0 or 20.0 mm lactic acid, 30 min before and during the same standard hypoxic insult. following at 30-min recovery period the ability of these slices to respond to orthodromi ...	1988	3345436
oxygen-derived free radicals in cerulein-induced acute pancreatitis.	conscious rats were treated with a supramaximal dose of 5.10(-6)g.kg-1.h-1 of cerulein for periods of 3 and 12 h. in both groups of animals typical features of acute oedematous pancreatitis were proved by biochemical and histologic examinations. the most important finding of our study was the decrease of superoxide dismutase (sod) activity in pancreatic tissue, accompanied by a slight increase of this scavenger enzyme in serum of rats stimulated with cerulein during 3 h. parallelly, evident elev ...	1988	3249921
increased concentrations of tyrosine and phenylalanine in the cerebrospinal fluid as a possible reflection of related changes of the brain tissue in newborns with intra-uterine growth retardation.	plasma and cerebrospinal fluid concentrations of tyrosine and phenylalanine were measured fluorometrically in human term neonates appropriate for gestational age and in neonates small for gestational age with severe intra-uterine growth retardation of type ii. the intra-uterine retarded newborns showed significant differences of both plasma and cerebrospinal fluid tyrosine levels as compared with the non-retarded newborns: the mean plasma and cerebrospinal fluid tyrosine were determined to be 2. ...	1988	3254150
laboratory evaluation of essential oil of mentha piperita linn. against trichophyton mentagrophytes.		1988	3255748
lectin binding patterns of the human cornea. comparison of frozen and paraffin sections.	twelve fitc-conjugated lectins were used to establish a reaction pattern with cellular and noncellular components in fresh-frozen and formalin-fixed paraffin sections of 11 normal human corneas. in frozen sections the epithelium demonstrated the most active lectin staining; keratocytes and endothelium stained to a lesser degree. of the noncellular components, the epithelial basement membrane and descemet's membrane of some of the tissues were stained most by phasolus vulgaris agglutinin (pha), p ...	1988	3219860
economic value of vitamin a and exhelm (morantel tartrate) in sheep in niger.		1988	3232646
aetiological spectrum of viral hepatitis in hospitalised adult patients in calcutta.		1988	3248987
[parasitic gastrointestinal nematodes of game ungulates in the belgian ardennes].		1988	3375795
[evaluation of the results of performance tests for stallions and the swiss saddle horse shows].		1988	3375797
a direct microassay for serum retinol (vitamin a alcohol) by using size-exclusion high-pressure liquid chromatography with fluorescence detection.	serum retinol (bound to plasma retinol-binding protein, rbp) can be determined by direct injection of as little as 20 microliter of serum or plasma by using size-exclusion high-pressure liquid chromatography (se-hplc) with fluorescence detection. toyo soda tsk g-3000sw columns (0.75 x 7.5-cm guard column plus 0.75 x 30-cm analytical column) were eluted with 0.2 m nacl/0.01 m phosphate buffer (ph 6.8) at 1 ml/min, with detection at 280 nm for protein elution. fluorescence of the retinol-rbp compl ...	1988	3407933
nutritional requirements of shigellae for growth in a minimal medium.	most (about 81%) of the clinical isolates of shigellae that were tested failed to grow in a minimal medium. of the auxotrophic isolates belonging to the four shigella species, 98% grew in a minimal medium containing methionine, nicotinic acid, and tryptophan. the combination of methionine and tryptophan appears to be an obligatory requirement for shigella dysenteriae serotype 1 strains, while the combination of nicotinic acid and tryptophan appears to be obligatory for serotype 2. requirements w ...	1988	3346071
variation in the human lymphocyte sister-chromatid exchange frequency: results of a long-term longitudinal study.	the variation in lymphocyte sister-chromatid exchange (sce) frequency as a function of time was investigated in nonsmokers and smokers. the smokers were divided into 3 groups depending on their smoking status. the group termed 'smokers' participated in a program to stop smoking but did not reduce or eliminate their use of tobacco; 'smoke enders' successfully completed the smokending program and remained free of tobacco for the duration of the study, while the 'variable' group stopped smoking for ...	1988	3347215
knobbed spermatozoa in two normande bulls.	two unrelated yearling normande bulls in a bull stud had 26 and 18% knobbed spermatozoa as well as other major sperm abnormalities. one of the bulls was eliminated from the bull stud because of poor semen quality and growth performance. the other bull was maintained for additional semen evaluation, during which time the mean percentage of major sperm abnormalities decreased, indicating amelioration of a transient testicular degeneration.	1988	3417539
clinical trial of low-fat, high-carbohydrate diet in subjects with mammographic dysplasia: report of early outcomes.	despite evidence that dietary fat intake may influence breast cancer risk, there is little information about the effects of dietary fat on the human breast. we have studied the effects of dietary fat on the breast by examining the influence of dietary fat reduction on mammographic dysplasia (nodular or sheetlike areas of radiological density). subjects with mammographic dysplasia were randomly allocated to a control group, in which they received advice about maintaining a balanced diet (36% of c ...	1988	3418730
pressure sores: a biochemical test for early detection of tissue damage.	this study was undertaken to seek biochemical indicators in blood associated with the onset of early tissue damage which may progress to the formation of a pressure sore. changes in serum creatinine phosphokinase (cpk), inorganic phosphate, and lactate dehydrogenase in systemic blood of pigs were investigated before, during, and after local indentation. pressures of 540mmhg and 700mmhg were applied for six hours on both sides of the scapula and backs of five anesthetized animals. two hours after ...	1988	3421821
[functioning of inhibitory mechanisms of gastric secretion during variations in its intensity].	seasonal and ovulatory fluctuations of "alimentary" gastric secretion were accompanied by synphase changes of its fatty inhibition in dogs with pavlov gastric pouch and with basov fistula of the stomach. secretory variations stimulated with histamine led to no such inhibitory fluctuations. changes in functioning of stimulating mechanisms of the gastric gland seem to be accompanied by parallel changes of functioning of inhibitory ones in natural conditions. an increase or decrease of acid gastric ...	1988	3356274
a comparison of the effects of measured, predicted, estimated and constant residual volumes on the body density of female athletes.	the body density (bd), and hence the relative body fat (% bf) was measured for 182 female athletes. the residual volume (rv) was determined both before and after the underwater weighing by a multiple breath helium dilution technique with the subject immersed to neck level. the absolute mean difference (lxdl) and see between the two rv trials were 63 and 75 ml, respectively. these increased to values ranging 144-685 and 187-252 ml, respectively, when the mean of the two rv trials for each subject ...	1988	3366515
genetic characterization of leishmania isolates at 37 enzyme loci.		1988	3417374
[familial occurence of osteosarcoma (author's transl)].		1988	266820
[legal aspects: are the hands which provide care harmless].		1988	259575
inclusion vs. exclusion of the female in education of the male on the resumption of sex post first myocardial infarction.		1988	259408
[comparative data from radioisotopic and x-ray methods of study in osteogenic group tumors].	a comparative analysis of the data of roentgenography-angiography, scanning and radiometry with strontium-85 in 54 patients showing osteogenic sarcoma and osteoblastoclastoma has demonstrated a relationship between the quantitative characteristics of the radioactive sr inclusion and the intensity of reactive osteoformation, mineralization of neoplastic tissues, an activity of the pathological process, and the rate of vascularization, as well.	1988	266309
guidance on symptoms of illness in babies: a further service from the fsid.		1988	259593
mandibular staple bone plate implant and prosthesis. report of a case.	a case treated by the mandibular staple implant fastener system and prosthesis is presented. the phases of therapy, involving preprosthetic surgical preparation, construction of conventional dentures, implantation of the orthopedic appliance, preparation of an interim postsurgical prosthesis, and construction of the definitive prosthesis, are described and illustrated.	1988	266676
parallel studies of hla antigens in patients with rheumatic heart disease and scleritis: comparisons with three control populations.	parallel studies were carried out on hla antigens in patients with rheumatic heart disease and scleritis. comparison with one control population showed a significant excess of bw15 in both disease samples, while a comparison with two other control populations, showed the excess not to be significant. possible reasons for this discrepancy are discussed, together with the effect on statistical significance of a small percentage of false antigen assignments in one of the samples. a small systematic ...	1988	266606
hla b27 in ankylosing spondylitis: differences in frequency and relative risk in american blacks and caucasians.	twenty-eight hla alleles of the a and b loci were determined in 23 american blacks and 50 caucasians with primary ankylosing spondylitis (as). the prevalence of hla b27 was significantly increased in american black patients (48 per cent) vs black controls (two per cent), but was much less than the 94 per cent found in caucasian patients (controls eight per cent). the lower prevalence of b27 in american black patients vs caucasian patients was significant (p less than 0.001), and indicated that s ...	1988	266595
recent advances in genetic counselling.		1988	259242
postoperative scars and cleavage lines.	we elucidated the pattern of skin cleavage lines of the neck of japanese cadavers and studied the scarring in 42 cases of radical neck dissection for the removal of metastatic nodules from the malignant tumors of the oral region, in the operation of which one incision was made parallel to, and another perpendicular to, the cleavage lines. from this study we came to the following conclusions: 1) the cleavage lines run parallel in ring-form around the neck, with the exception of the back and the l ...	1989	267516
primary aldosteronism-recognition and management.		1989	267288
[estrogen therapy and carcinoma of the endometrium (author's transl)].	the public discussion and the discussion among gynaecologists about the possible increased risk to develop carcinoma of the endometrium following post menopausal treatment with estrogens has lead to insecurity and restricted attitudes in the prescriptions of estrogens. in a statistic comparison of matched pairs of 130 cases of carcinoma of the endometrium with an equal number of selected control cases the following findings were elucidated: 1. any estrogen preparations do not increase the relati ...	1989	210085
relationship of oral complications to peripheral blood leukocyte and platelet counts in patients receiving cancer chemotherapy.	patients undergoing cancer chemotherapy often suffer from oral complications as a result of their disease and its treatment. the effects of the chemotherapy on the bone marrow and oral mucosa, coupled with the patient's immunosuppressed state and altered oral microbial flora, predispose these patients to oral mucositis, infection, and hemorrhage. the oral mucosa appears to mirror the effects of the chemotherapy on the bone marrow, as there appears to be a direct relationship between the changing ...	1989	313547
possible torsade in a patient with aids.		1989	2115530
occlusal and craniofacial growth changes during puberty.		1989	267438
sex- and age-related changes in catecholamine metabolism and release of rat adrenal gland.	to examine the possible existence of changes in the adrenal catecholaminergic activity during aging, we analyzed the adrenal content of catecholamines (ca) and the activities of selected enzymes involved in their metabolism as well as the basal and the k+-stimulated release of these ca in incubated adrenal tissue of aged (greater than 22 months) and young (2 months) rats of both sexes. adrenal contents of norepinephrine (ne) and epinephrine (e) of male rats were unaltered in aging, although aged ...	1989	2812194
[drug resistance of the causative agent of grigo'rev-shiga dysentery (shigella dysenteriae 1)isolated in the ussr].	forty strains of s. dysenteriae 1 isolated in the ussr within 1986-1988 were tested for their resistance to 11 antibacterial drugs. it was shown that 92.5-97.5 per cent of the isolates were resistant to chloramphenicol (cm) and tetracycline (tc), 22.5 per cent to streptomycin (sm), 17.5 per cent to nalidixic acid (nal) and 10 per cent to ampicillin (ap) and polymyxin (pm). resistance to cm tc (51.4 per cent) and cm tc nal (13.5 per cent) represented the predominating phenotype. 35 per cent of th ...	1989	2694989
characterization and autoprocessing of precursor and mature forms of human immunodeficiency virus type 1 (hiv 1) protease purified from escherichia coli.	a recombinant plasmid encompassing the human immunodeficiency virus type 1 (hiv 1) protease coding sequence and flanking regions (ala-13 to gly-185 of the pol open reading frame) has been expressed in two distinct strains of escherichia coli, ar58 and ar68. in the first strain, ar58, the primary translation product, a 25 kilodalton (kda) precursor protein, is short-lived and rapidly processes itself to the 11 kda mature protease in vivo. in the second strain, ar68, the 25 kda species is only par ...	1989	2695927
[the etiological structure of shigellosis in the ussr in recent years].	the characterization of etiological structure of shigella infection in the whole of the ussr, in individual union republics and at a number of other administrative territories of the ussr in recent years is presented. s. flexneri has been shown to prevail at the territories with unsatisfactory water supply of the population, and s. sonnei prevails at the territories with good water supply. at the former territories s. dysenteriae and s. boydii retain their etiological importance, while at the la ...	1989	2698030
[development of antibiotic resistance of type 1 shigella dysenteriae strains (shiga bacillus) isolated in tananarive on the east coast of madagascar].	from november 1988 to march 1989, 804 malagasy children stools were studied and 37 shigella strains isolated. 5 shigella dysenteriae type 1 from malagasy east coast (mananjary), presented a multiply resistance to ampicillin, carbenicillin, streptomycin, chloramphenicol, tetracycline, sulphonamides and trimethoprim. this last resistance has recently appeared in this area.	1989	2699224
thromboxane synthase activity and platelet function after furegrelate administration in man.	furegrelate sodium (u-63,557a), a pyridine-derivative thromboxane synthase inhibitor, was administered orally in single doses of 200 to 1600 mg to normal male subjects. furegrelate produced a dose-related inhibition of thromboxane synthesis for 8-12 hours when measured either ex vivo from platelet-rich plasma (prp) or in vivo from urine. in general, the extent of thromboxane synthesis inhibition was greater in prp than in urine. furegrelate significantly inhibited platelet aggregation, but the e ...	1989	2708549
immunoblotting analysis of the reaction of wildebeest, sheep and cattle sera with the structural antigens of alcelaphine herpesvirus-1 (malignant catarrhal fever virus).	malignant catarrhal fever (mcf) is a disease of cattle and some other ruminants caused by alcelaphine herpesvirus-1 (ahv-1), a virus of wildebeest. the disease also occurs in the absence of wildebeest and is then thought to be caused by a viral agent harboured by the sheep. the structural proteins of ahv-1 have been used as antigens for the immunoblotting analysis of sera from wildebeest, sheep and cattle infected by either ahv-1 or the "sheep-associated" form of the disease. wildebeest sera sho ...	1989	2718352
detoxification of lithocholic acid. elucidation of the pathways of oxidative metabolism in rat liver microsomes.	the hydroxylation of lithocholic acid (3 alpha-hydroxy-5 beta-cholanoic acid) by adult male sprague-dawley rat liver microsomes supplemented with nadph was studied. metabolites were separated by a combination of thin-layer chromatography and high pressure liquid chromatography, both with and without prior methylation and acetylation of the samples. the resulting products were characterized by thin-layer, gas-liquid, and high pressure liquid chromatography by comparison with authentic bile acid s ...	1989	2794781
fast elisa for measuring serum antibody responses.	a method which speeds up the enzyme-linked immunosorbent assay (elisa) is described. the procedure uses a modified falcon fast assay screening system (becton dickinson labware, lincoln park, nj) and falcon round-bottom 96-well plates. antigen is adsorbed onto beads which extend from a lid and fit into 96-well plates. the beads are washed in a trough and reacted to antibody in the round-bottom plate. the labor required to wash the plates after coating with antigen, antibody or conjugate is thereb ...	1989	2723439
analytical determination of methylated histidine in proteins: actin methylation.	the methylation of histidine in actin from various muscle and nonmuscle sources has been studied by formation of phenylthiocarbamyl derivatives and subsequent reverse-phase high-pressure liquid chromatographic separation and analysis of actin hydrolyzates. all the actin species examined were found to contain 3-methylhistidine. this method has also been used in assays for the enzyme(s) responsible for methylation of rabbit skeletal muscle actin and to investigate the formation of other methylated ...	1989	2729572
in vitro and in vivo comparison of the abilities of purine and pyrimidine 2',3'-dideoxynucleosides to inhibit duck hepadnavirus.	four purine and two pyrimidine 2',3'-dideoxynucleosides were studied for their ability to inhibit duck hepadnavirus replication. the purine 2',3'-dideoxynucleosides were more potent antiviral agents than the pyrimidine 2',3'-dideoxynucleosides. the concentration for 50% inhibition of viral replication (ic50) was determined for each of the effective agents. two drugs with low ic50s, 2,6-diaminopurine 2',3'-dideoxyriboside and 2',3'-dideoxyadenosine, were chosen for in vivo studies. animals receiv ...	1989	2729928
cockroaches (periplaneta americana) as carriers of agents of bacterial diarrhoea in accra, ghana.	bodies and intestinal contents of 208 cockroaches (periplaneta americana), collected from kitchens in accra and some surrounding villages were cultured for enteric bacterial pathogens. six of them harboured three different serogroups of salmonella, one had shigella dysenteriae, 64 had coliforms, 13 had proteus species, two had pseudomonas species and the rest (122) carried none of the bacterial species mentioned above. the presence of salmonella species shigella dysenteriae and coliforms in thes ...	1989	2611875
[acute maternal anterior poliomyelitis in a non-endemic zone].	the authors report the case of a 26 year old woman with acute anterior poliomyelitis contracted during the vaccination of her baby. despite having been herself vaccinated in infancy she was not protected against the poliovirus. the clinical interest of this uncommon case is a severe paralytic state with definitive paraplegia. the authors suggest serologic testing of patients born before 1967 especially if they are at risk of encountering the virus.	1989	2561040
isolation of inhibitors of adenylate cyclase from dan-shen, the root of salvia miltiorrhiza.	the effect of dan-shen extract, the root of salvia miltiorrhiza, on adenylate cyclase was investigated in both rat brain and rat erythrocytes. the etoac fraction of the meoh extract was proved to have significant inhibitory activity. potent inhibitory principles in the etoac fraction were isolated and identified as 4 polyphenolic acids, rosemarinic acid, lithospermic acid, and their methyl ester derivatives.	1989	2630095
aging effects on secretory iga immune responses.	studies of cellular and humoral components of the secretory immune system indicated dramatic decreases in the siga response to t-dependent antigens (i.e. dnp-bgg) in aged rats. senescent rats (18-20 months old) showed comparable levels of siga anti-dnp antibody to adult animals after oral immunization with a t-independent antigen, dnp-ficoll. the salivary siga responses to both antigens were substantially decreased in weanling rats (21-35 days) versus the adult rats. significant anamnestic siga ...	1989	2786502
relationship between multiple drug-resistance & enterotoxin production by shigella species.	culture filtrates prepared with strains of shigella dysenteriae 1 and those of sh. flexneri and sh. sonnei, collected from different geographical locations in india caused accumulation of fluid in rabbit gut loops, indicating their capability to produce shiga and shiga-like toxins respectively. sh. boydii strains were noted for the first time to produce shiga-like toxins. the failure of production of fluid accumulation in rabbit gut by shigella strains that lacked the r-markers of the antibiotic ...	1989	2737704
perioral anesthesia disrupts maternal behavior during early lactation in long-evans rats.	the role of perioral somatosensory stimulation from pups in maternal behavior was investigated in lactating long-evans rats (rattus norvegicus) during early lactation by local anesthesia of the mystacial pads with lidocaine, which blocks conduction in the infraorbital nerve. it was concluded that (1) acute perioral anesthesia markedly inhibited pup retrieval when the subjects had no formal pretreatment retrieval experience. further, these dams displayed little tolerance to the lidocaine treatmen ...	1989	2757582
experimental studies on membrane permeability defects of drug-resistant bacteria.	multiply antibiotic-, lysozyme-and bacitracin-resistant, representative strains of staphylococci, escherichia coli, shigella dysenteriae and klebsiella pneumoniae were compared respectively with their parent cultures in respect of their membrane permeability as judged by fluorescence polarization spectroscopy, using a sensitive fluorescent probe. the cytoplasmic membrane of the drug-resistant mutants was found to be less fluid and permeable, compared to that from the sensitive wild types. the st ...	1989	2807429
relationship between concentration and anticonvulsant effect of phenytoin against electroshock-induced seizures in rats: comparison of sampling sites for concentration determinations.	the purpose of this investigation was to determine the optimum sampling site for phenytoin concentration measurements in the context of pharmacodynamic studies of the anticonvulsant effect of phenytoin. determination of drug concentrations in the serum, serum water, brain, and cerebrospinal fluid (csf) of rats as a function of time after iv injection of a 6-mg/kg dose revealed a significant disequilibrium between brain and serum water for 15 min and between csf and serum water for 5 min after in ...	1989	2760817
determination of estradiol 2- and 16-alpha-hydroxylase activities in mcf-7 human breast cancer cells in culture using radiometric analysis.	using a radiometric assay the effects of estradiol upon the activity of estradiol 2- and 16 alpha-hydroxylases in mcf-7 human breast cancer cells in culture were studied. after 5 days of treatment and 36 h of withdrawal, incubation in the presence of either 2- or 16 alpha-tritiated substrate was carried out. estradiol (10 nm) significantly increased 16 alpha-hydroxylase activity (21%, p less than 0.01), while no effects on 2-hydroxylase activity was observed. treatment for 6 weeks caused a major ...	1989	2770303
twenty-year comparison of the human allograft and porcine xenograft.	this study compares a retrospective consecutive series of human allografts and concurrent porcine xenografts implanted over a 10-year interval. there were 571 allograft valves and 1,351 xenograft valves implanted in the aortic and mitral position with mean follow-up of 12.8 years for the allografts and 6.2 years for the xenografts. this study compares the incidence of structural deterioration over long-term follow-up. we found that there was no significant difference between four manufacturers o ...	1989	2774760
a strategy for high-volume sequencing of cosmid dnas: random and directed priming with a library of oligonucleotides.	direct sequencing of cosmid dnas using a library of oligonucleotide primers of length 8, 9, or 10 is proposed. the statistics of priming indicate that a primer library sufficient for determining the sequence of the entire human genome (100,000 cosmids) would be small enough to be assembled and managed. such a library would greatly reduce the cost and effort of high-volume sequencing: primers would be instantly available; the sequence of each cosmid dna could be determined from a single dna prepa ...	1989	2780548
purification of shiga toxin and shiga-like toxins i and ii by receptor analog affinity chromatography with immobilized p1 glycoprotein and production of cross-reactive monoclonal antibodies.	shiga toxin from shigella dysenteriae 60r was purified to homogeneity by a novel one-step receptor analog affinity chromatography method. the method was based on the binding affinity of shiga toxin for a specific disaccharide, gal alpha 1----4gal, which was also present in glycoproteins with p1 blood group seroreactivity produced in hydatid cysts from sheep infected with echinococcus granulosus. having shown that cyst fluid p1 glycoprotein bound shiga toxin on a solid phase, a p1 glycoprotein af ...	1989	2807552
co-localization of corticotropin-releasing factor- and enkephalin-like immunoreactivities in nerve cells of the rat hypothalamus and adjacent areas.	the co-localization of corticotropin-releasing factor (crf)- and enkephalin (enk)-like immunoreactivities in nerve cells of the rat hypothalamus and adjacent areas was investigated by the simultaneous application of immuno-beta-galactosidase staining and the peroxidase-antiperoxidase (pap) method to the same sections. crf-like immunoreactive cells were stained blue with immuno-beta-galactosidase staining and enk-like immunoreactive cells brown with the pap method. double-labeled cells with overl ...	1989	2786446
lymphokine-activated killer (lak) cells inhibit the clonogenic growth of human leukemic stem cells.	the effect of lymphokine-activated killer (lak) cells on the in vitro clonogenic capacity of acute myeloid leukemia (aml) blasts was investigated in a semisolid medium assay. the leukemic clonogenic capacity of 11 aml cases, selected on the basis of their ability to grow in vitro, was highly reduced following overnight preincubation with lak effectors. the degree of colony inhibition, which ranged between 66% and 98% (mean 83.8% +/- 11.4 sd), was quantitatively greater than by 51cr release, whic ...	1989	2786477
investigations of the potential for five beta-lactam antibiotics to elicit type ii hypersensitivity reactions in rats and monkeys.	immunologic reactions are occasionally elicited in patients by various beta-lactam antibiotics (e.g., penicillins and cephalosporins). a relatively rare reaction (type ii hypersensitivity) may involve antibody-mediated destruction of erythrocytes, leukocytes, and/or platelets. during the safety evaluation of several modified beta-lactam compounds (carbapenems), hemolytic anemia and/or neutropenia were observed in rhesus monkeys, and anemia, neutropenia, and thrombocytopenia in rats, after approx ...	1989	2786479
the renal phosphate transport defect in normal mice parabiosed to x-linked hypophosphatemic mice persists after parathyroidectomy.	the x-linked hypophosphatemic (hyp) mouse is a model for human x-linked hypophosphatemia. surgical joining of normal to hyp mice by parabiosis results in the normal mice developing low renal retention of phosphate and hypophosphatemia. these results suggest a humoral component to the renal defect. to test whether this component could be parathyroid hormone, surgical parathyroidectomy (ptx) or sham surgery was performed in mice 3 weeks after parabiotic union (n greater than 20 per group). after a ...	1989	2816501
effect of detoxification processes on the interferon-inducing activity of bacterial endotoxins.	the interferon (ifn)-inducing activity of detoxified lipopolysaccharide (lps) was tested in rabbits treated with lps preparation derived from escherichia coli, salmonella typhi, salmonella enteritidis and shigella dysenteriae serovar 1. of the detoxification procedures used, alkaline hydrolysis, hydroxylaminolysis, formalization, treatment with sodium deoxycholate and the radiodetoxification (fast or slow) methods had no appreciable effects on the ifn-inducing potential of lps. in contrast, acet ...	1989	2484274
effects of the nmda receptor antagonist mk-801 against amygdaloid-kindled seizures in the rat.	the effects of acute and subchronic administration of the noncompetitive nmda receptor antagonist mk-801 on the duration and severity of amygdaloid-kindled seizures in rats were determined to evaluate its anticonvulsant activity. both the highest acute (1000 micrograms/kg) and subchronic regimens (2 x 300 micrograms/kg, 7 days) produced significant reductions in the seizure stage and afterdischarge duration. although ataxia may have affected the seizure stage, mk-801 attenuated the primary after ...	1989	2536332
[in vitro susceptibility of campylobacter jejuni and campylobacter coli to quinolone antibiotics].	in this study, in vitro susceptibilities of 24 campylobacter strains isolated from fecal samples were tested for quinolone antibiotics. 13 of the strains were identified as c. jejuni and 11 of them were c. coli. antibiotics which were included in this study were ciprofloxacin, ofloxacin, pefloxacin and fleroxacin. antibiotic susceptibility tests were performed on mueller-hinton agar (supplemented with 5% sheep blood) using the agar dilution method. results showed that quinolone antibiotics are v ...	1989	2518646
[imported myiasis: analysis of 5 cases].	five cases of furuncular myiasis imported by spanish travelers from tropical countries are reported. after reviewing the cycle of the infecting organism and the characteristics of the human disease, a table with the clinical and epidemiological data of our patients is presented. the relevance of an adequate clinical and geographical anamnesis in these patients is discussed. finally, the effectiveness of a simple, nonaggressive treatment is reported, which permits the patient to verify by himself ...	1989	2490857
hemagglutinating properties of shigella dysenteriae type 1 and other shigella species.	strains of shigella dysenteriae type 1 cultured in casamino acids-yeast extract broth medium in the presence of 1 mm calcium chloride at 37 degrees c for 22 h induced hemagglutination of erythrocytes that was inhibited by n-acetylneuraminic acid, n-acetylneuramin-lactose, and alpha 1-glycoprotein. the hemagglutination was heat labile, and the absence of cell-surface appendages suggested a nonfimbrial adhesin(s). under the same conditions, strains of shigella flexneri (types 1a, 1b, 2a, and 2b) s ...	1989	2503449
improved sexual function in hemodialysis patients on recombinant erythropoietin: a possible role for prolactin.	as it was reported that correction of anemia in long-term hemodialysis patients by recombinant human erythropoietin (r-huepo) is associated with improved sexual function, we conducted the present study to further delineate the mechanism(s) by which this is brought about. serum prolactin, testosterone, and parathyroid hormone (pth) levels were followed during 4 months of r-huepo therapy. within 4 months of treatment with r-huepo, hematocrit values rose from 23.7 +/- 1.2 to 35.7 +/- 0.2% and hemog ...	1989	2914405
the protective effect of thromboxane synthetase inhibition on renal function in systemic sepsis.	to study the role of thromboxane in systemic sepsis and renal failure, peritonitis was induced surgically in 22 sheep, leading to local and systemic sepsis. a selective thromboxane synthetase inhibitor, u63,557a (upjohn co, kalamazoo, mi) was given before surgery in five animals and 30 minutes after surgery in five animals. a typical picture of volume-loaded, normotensive, vasodilated septic shock developed in all animals. twenty four hours after induction of sepsis, the control group showed a m ...	1989	2916566
sensitivity of campylobacter pylori to colloidal bismuth subcitrate.		1989	2921366
three potassium currents in mouse motor nerve terminals.	a study of the k conductance of the presynaptic membrane has been performed in the triangularis sterni muscle of the mouse. external currents generated in the presynaptic terminals upon invasion by action potentials have been recorded using microelectrodes inserted into the perineurium of preterminal nerve bundles. the voltage-dependent k current could be pharmacologically dissected into fast (ikf) and slow (iks) components. while both are sensitive to 3,4-diaminopyridine (3,4-dap), only ikf is ...	1989	2928092
nontraditional pedagogy for implementing human genetics education.		1989	2929601
adp-ribosylatable content of elongation factor-2 changes during cell cycle of normal and cancerous human cells.	the amount of protein elongation factor ef-2 that can be inactivated by diphtheria toxin-mediated adp-ribosylation, a measure of its active content, decreases by 45% and 66% in g1-arrested normal human fibroblasts and in hela cells respectively. on restimulation of cells with fresh serum, the amounts of adp-ribosylatable ef-2 begin to increase within 4 h. whereas the level of active ef-2 returns to normal (exponential phase of growth) in 20 h in the case of fibroblasts, only 47% recovery was obs ...	1989	2930555
arthrodesis of metatarsophalangeal joints in a dog.	chronic osteomyelitis and septic arthritis involving the 3rd and 4th metatarsophalangeal joints were diagnosed in a doberman pinscher. arthrodesis of the 3rd and 4th metatarsophalangeal joints resulted in a subtle persistent gait abnormality, but limb function was preserved. arthrodesis of metatarsophalangeal joints is an alternative to amputation, especially when main weight-bearing digits are involved.	1989	2914800
production and characterisation of monoclonal antibodies to verotoxins 1 and 2 from escherichia coli of serotype o 157:h7.	fourteen hybridoma cell lines were isolated that produced monoclonal antibodies (mabs) to purified verotoxins 1 and 2 (vt1, vt2) of escherichia coli of serotype o157:h7. of these mabs, eight were obtained by immunisation of balb/c mice with purified vt1, and six were obtained from balb/c mice immunised with purified vt2. with the exception of mab 1c5, with a heavy chain of igg2b class, antibodies produced from mice immunised with heat-treated toxin were of igm class. mabs produced from mice immu ...	1989	2479749
studies on shiga-like toxin produced by enterohemorrhagic escherichia coli: purification and characterization of the toxin and development of methods for identifying the toxin.	a simple purification method using deae cellulose column chromatography and immunoaffinity column chromatography was developed for purifying shiga-like toxin produced by escherichia coli o157:h7. about 0.75 mg of purified toxin was obtained from 5 liters of culture (62% recovery). the purified toxin was demonstrated to be immunologically, biologically and structurally indistinguishable from shiga toxin. a sandwich enzyme-linked immunosorbent assay (elisa) was developed for detection of shiga-lik ...	1989	2485395
renin: endocrine, paracrine, or part-paracrine control of blood pressure?		1989	2650710
pathogenesis of shigella diarrhea. xiv. analysis of shiga toxin receptors on cloned hela cells.	binding kinetics of shiga toxin to hela ccl-2 cells and to cell lines cloned by limiting dilutions were determined. lines with a wide range of sensitivity to shiga toxin were obtained. binding data, analyzed by a computer-based scatchard model program, revealed two classes of binding sites, one of low affinity and high capacity and one of high affinity and low capacity. the number of high affinity, but not low affinity, sites present on the clones correlated with their sensitivity to toxin. tuni ...	1989	2651533
nonrandom rearrangement of chromosome 14 at band q32.33 in human lymphoid malignancies with mature b-cell phenotype.	chromosomes were studied in 61 patients with differentiated b-cell malignancies including 21 with non-hodgkin's lymphoma (nhl), three with hairy cell leukemia (hcl), eight with waldenström's macroglobulinemia (wm), and 29 with plasma cell disorder. chromosomally abnormal clones were identified in 35 of 61 patients studied: all with nhl, all with hcl, three of eight with wm, and eight of 29 with plasma cell disorder. the most recurrent chromosomal abnormality, observed in 26 of the 35 patients wh ...	1989	2492904
four new provisional serovars of shigella.	four bacterial strains are described that possess the biochemical characteristics of shigella species but do not belong to any of the established shigella serovars or to any previously described provisional serovar. one strain fermented mannitol, and it is proposed that this be the type strain for a new provisional serovar of shigella boydii. the remaining strains did not ferment mannitol and belonged to three different serovars. these strains are proposed as type strains for three new provision ...	1989	2501349
unusual nucleotide arrangement with repeated sequences in the escherichia coli k-12 chromosome.	between 59 and 60 min on the escherichia coli genetic map, there is a highly conserved sequence of 29 base pairs, containing an inverted repeat of seven base pairs that appears 14 times, 32 or 33 base pairs apart, downstream of the iap gene coding region. about 24 kilobase pairs downstream of the 14 repeats, a similar 29-base-pair sequence with a spacing of 32 base pairs appears seven times. nucleotide sequences hybridizing with the 29-base-pair fragment were also detected in shigella dysenteria ...	1989	2656660
[increased efficiency in the disinfection of sewage by lactic acid bacteria].	the antimicrobic properties have been studied in 30 strains of lactobacilli. as a result a strain, the strongest antagonist relative to choleric vibrios and other enteropathogenic microorganisms, is selected. lactobacilli are found to retain their viability and biological activity in the activated sludge during the whole period of observation (6 months). biological disinfection of sewage is shown possible to be intensified using the activated sludge inoculated by the culture of the selected lact ...	1989	2514340
identification of shiga-like toxin in escherichia coli strains, etiological agents of diarrheal disease.	shiga-like toxin presence, in 20 e. coli strains, etiological agents of diarrheal diseases, is studied by preparing extracts at +4 degrees c, in the presence of chloroform and by i.v. inoculation in mice. in 4 out of 20 strains, shiga-like toxin in high titres was identified. most of the strains presented an inconstant and variable production of shiga-like toxin in comparison with shigella dysenteriae type 1 (shiga) reference strain. the authors also confirm the existence of shiga-like toxin und ...	1989	2520669
[the dependence of hemoglobin and iron reserve indices on habitat conditions (sampling research in osh province, the kirghiz ssr)].	sampling investigation of female population (896 subjects) was conducted in five regions with opposite environmental conditions. a relatively low incidence of iron deficiency anemia and high incidence of exhausted iron reserves in the women living in the high mountains indicates the participation of the adaptive-compensatory mechanisms in the development of iron-deficiency. a high rate of iron deficiency incidence in the regions of intensive tobacco growing and association of iron-deficiency ane ...	1989	2533125
multiple cooperative interactions constrain bpv-1 e2 dependent activation of transcription.	transcription directed by the bpv-1 long control region (lcr) is conditional upon activation by the virally encoded e2 protein. within the 1.0 kb lcr there are five separate regions, a to e, that contain e2 responsive enhancers. the smallest functional region, a, is only 38 bp and contains two copies of the consensus sequence acc(n)6ggt that is known to function as an e2 binding site in vitro. we show that a pair of these constitutes a minimal functional e2 responsive enhancer element but that t ...	1989	2542891
dual function of a nuclear factor i binding site in mmtv transcription regulation.	using linker-scanning mutagenesis we had previously identified four elements within the mmtv ltr which are necessary for transcriptional stimulation by glucocorticoid hormones. two of them overlapped with regions to which the glucocorticoid receptor binds in vitro. the third element contained a nf-i binding site, and the fourth the tata box. here we show that mutations that abolish in vitro binding of nf-i had a negative effect also on the basal activity of the mmtv promoter of ltr-containing pl ...	1989	2542892
influence of diethyldithiocarbamate on nickel-induced hepatic metallothionein in rats.	the influence of sodium diethyldithiocarbamate (ddc) on 63nickel chloride-induced metallothionein (mt) and alterations in essential metal levels in the liver of rats was investigated. the induction of mt, zn and cu levels of the hepatic cytosolic "heat stable fraction" (hsf) by ddc increased with time up to 24 hr. although mt, zn and cu were significantly greater at 17 hr than those at 6.5 hr after 63ni administration, the ni level decreased. the treatment with ddc at 6 hr, but not at 10 min, pr ...	1989	2548306
phosphate regulon in members of the family enterobacteriaceae: comparison of the phob-phor operons of escherichia coli, shigella dysenteriae, and klebsiella pneumoniae.	the structure and function of the phob and phor genes of shigella dysenteriae strains and klebsiella pneumoniae, which are involved in regulation of the phosphate regulon, were analyzed. complementation tests among the genes of escherichia coli, s. dysenteriae strains, and k. pneumoniae for production of alkaline phosphatase indicate that s. dysenteriae serotype 2 and serotype 3 strains and k. pneumoniae are phoa+ phob+ phor+ but s. dysenteriae sh and serotype 1 strains are phoa phob+ phor. nucl ...	1989	2556368
characterization of the rela1 mutation and a comparison of rela1 with new rela null alleles in escherichia coli.	the most widely studied "relaxed" mutant of the rela locus, the rela1 allele, is shown here to consist of an is2 insertion between the 85th and 86th codons of the otherwise wild-type rela structural gene, which normally encodes a 743-amino acid (84 kda) protein. the rela protein is a ribosome-dependent atp:gtp (gdp) pyrophosphoryltransferase that is activated during the stringent response to amino acid starvation and thereby occasions the accumulation of guanosine 3',5'-bispyrophosphate (ppgpp). ...	1989	2556396
endocytosis from coated pits of shiga toxin: a glycolipid-binding protein from shigella dysenteriae 1.	evidence is presented that endocytosis is involved in the transport to the cytosol of the cytotoxin from shigella dysenteriae 1, shiga toxin, which acts by removal of a single adenine residue in 28-s ribosomal rna. inhibition of endocytosis by atp depletion of the cells prevented toxin uptake. exposure of hela s3 and vero cells to toxin at low extracellular ph, where translocation to the cytosol, but not endocytosis is inhibited, allowed the toxin to accumulate in a compartment where it was prot ...	1989	2564398
shigella dysenteriae type 1 infections in us travellers to mexico, 1988.	in 1988, the number of shigella dysenteriae type 1 (sd1) infections reported in the usa increased five-fold over the annual mean from the previous decade. 44 (94%) of 47 interviewed patients reported recent travel to mexico; 33 (75%) of these had been tourists to the yucatan peninsula. 27 patients who had travelled to mexico were admitted to hospital, of whom 2 had a haemolytic uraemic syndrome; none died. the antimicrobial resistance pattern and plasmid profile of the yucatan strain were simila ...	1989	2570242
expression and desensitisation of a2 purinoceptors on cultured bovine aortic endothelial cells.	purine nucleotides and their metabolites are important mediators of vascular tone. adenosine promotes relaxation of vascular smooth muscle, acting on the a2 subclass of purinoceptors. there is some evidence that these receptors are also present on vascular endothelial cells. we have therefore examined cultured aortic endothelial cells for adenosine (a2) sensitivity. bovine aortic endothelial cells (ag4762) were obtained from the institute of aging cell repository (usa), and cultured in monolayer ...	1989	2590913