Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [obtaining dysentery and salmonellosis diagnostica for the coagglutination reaction in lyophilized form and the elaboration of a rapid system]. | 1982 | 6182376 | |
| bacterial agents in acute diarrhea. | 1982 | 6191462 | |
| the nucleotide sequence coding for major outer membrane protein ompa of shigella dysenteriae. | the nucleotide sequence of the ompa gene from shigella dysenteriae has been determined and the amino acid sequence of the pro-ompa protein predicted. sequence comparison between the ompa genes of s.dysenteriae and escherichia coli showed that features such as mrna secondary structure and codon usage, as well as polypeptide function, have been conserved during evolution. the pro-ompa protein of s.dysenteriae consists of 351 residues, as opposed to the 346 of the e.coli protein and also shows seve ... | 1982 | 6283478 |
| increase in drug resistance among shigella dysenteriae, sh flexneri, and sh boydii. | 1982 | 6803969 | |
| [antigens of shigella dysenteriae serovars 3-7. iv. immunochemical study of the exotoxin]. | the exotoxins of sh. dysenteriae, serovars 3 and 7, possess antigenic and serological properties and are characterized by the heterogeneous antigenic structure which distinctly differs from the structure of o-antigen in its immunochemical properties. these exotoxins consist of thermolabile and thermostable components. the thermolabile exotoxin fraction is a lethal toxin. the thermostable exotoxin fraction, obtained from the cultures of sh. dysenteriae in the s-form, corresponds to o-antigen and ... | 1982 | 6808792 |
| production of shigella dysenteriae type 1-like cytotoxin by escherichia coli. | strains of escherichia coli previously implicated or proven to be causes of diarrhea were examined for production of a toxin similar to that of shigella dysenteriae type 1 (shiga). organisms grown in an iron-depleted broth were lysed by pressure disruption followed by ultracentrifugation. saline-dialyzed extracts were tested for cytotoxic effects on hela cells that were neutralizable with antiserum to shiga toxin. among the 13 e. coli strains so analyzed, 11 made a shiga-like cytotoxin in levels ... | 1982 | 6754826 |
| [bacillary dysentery - 1980]. | 1982 | 6755564 | |
| [use of coagglutination for serotyping shigella dysenteriae and shigella boydii]. | the coagglutination test was used to identify shigella boydii and shigella dysenteriae. a trial was carried out with 13 native rabbit antisera to s. boydii and 10 antisera to s. dysenteriae, as well as with coagglutinating reagents prepared from these antisera. the use of coagglutinating reagents was shown to ensure the complete specificity of the results, to prevent the adsorption of diagnostic antisera and to decrease their consumption 50 times. the importance of the coagglutination test for t ... | 1982 | 6760638 |
| bacterial adhesiveness and invasiveness in cell culture monolayer. 2. in vitro invasiveness of 45 strains belonging to the family enterobacteriaceae. | the invasive potential of 45 presumptive enteropathogenic and non-enteropathogenic bacterial strains belonging to the family enterobacteriaceae have been tested using the serény test and hep-2 cell monolayers examined by a combined light optical method. all the presumptive enteropathogenic strains of shigella dysenteriae, s. boydii, s. flexneri, s. sonnei and salmonella typhimurium showed in vitro invasiveness in the hep-2 cell culture test. fourteen presumptive non-enteropathogenic strains of e ... | 1982 | 6762798 |
| isolation of the shigella genus bacteria from the beach sand and water of the bay of gdańsk. | 1982 | 6764618 | |
| [anti-shigella dysenteriae 1 antibodies in the mexican population. their possible relation to the central american outbreaks of 1968-70]. | 1982 | 6765039 | |
| cloning and expression in escherichia coli k-12 of the genes for major outer membrane protein ompa from shigella dysenteriae, enterobacter aerogenes, and serratia marcescens. | the outer membranes of many gram-negative bacteria contain a major heat-modifiable protein which shows serological cross-reactivity with the ompa protein of escherichia coli k-12. using the cloned gene for the e. coli k12 protein as a dna-dna hybridization probe, we were able to identify the corresponding genes from shigella dysenteriae. enterobacter aerogenes, and serratia marcescens. these were cloned in a phage lambda vector, and their expression in e. coli k-12 was studied. all three ompa pr ... | 1982 | 7033204 |
| immunochemical and cytotoxic activities of shigella dysenteriae 1 (shiga) and shiga-like toxins. | toxins in culture supernatants and bacterial lysates of s. dysenteriae 1 and s. flexneri were quantitated by a cytotoxicity assay and a newly developed radioimmunoassay. cytotoxin titers paralleled toxin antigen levels. thus, variations in cytotoxicity among shigellae probably reflect differences in toxin yield rather than specific activity (cytotoxicity per microgram of toxin antigen). | 1982 | 7040247 |
| purification of a lethal toxin produced by shigella dysenteriae. | a lethal toxin was purified from the culture supernatant of shigella dysenteriae 1. the purification procedure utilized ammonium sulfate fractionation, column chromatography on deae-cellulose, cm-cellulose, hydroxylapatite and gel filtration on sephadex g-200. about a 4760-fold purification was achieved, with a yield of 2.7%. the purified lethal toxin appeared homogeneous on polyacrylamide gel disc electrophoresis. the ld50 of the purified lethal toxin in mice following i.p. injection was about ... | 1982 | 7043787 |
| expression in escherichia coli k12 of the cloned genes for a major outer membrane protein (ompa protein) from shigella dysenteriae, enterobacter aerogenes and serratia marcescens. | 1982 | 7044211 | |
| structural studies on a polysaccharide from shigella dysenteriae type 7. | the polysaccharide obtained from the o-somatic antigen of shigella dysenteriae type 7 (strain nctc 519/66) contains d-glucose, d-galactose, and 2-acetamido-2-deoxy-d-glucose in the mole ratios of 2:1-1. from the results of methylation, periodate oxidation, graded hydrolysis, and deamination studies, the structure assigned to the repeating unit of the polysaccharide is as follows, (formula: see text) oxidation studies with chromium trioxide revealed the nature of the anomeric linkages of some of ... | 1982 | 7046934 |
| purification and biological characterization of shiga toxin from shigella dysenteriae 1. | shiga toxin has been purified in milligram quantities to near homogeneity from cell lysates of shigella dysenteriae 1 strain 3818-0. purification involved an initial ultracentrifugation, ammonium sulfate fractionation, chromatography on deae-cellulose and carboxymethyl cellulose, gel filtration, and preparative isoelectric focusing in sucrose gradients. the purified toxin was resolved by discontinuous polyacrylamide gel electrophoresis into a major cytotoxic protein band and a closely migrating, ... | 1982 | 7047403 |
| enumeration and identification of pathogenic bacteria from sewage in kuwait. | mycobacterium spp. was isolated and enumerated in all sewage samples at different stages of the sewage treatment. aeromonas hydrophila, salmonella spp., shigella dysenteriae and others were isolated from both treated and untreated sewage. moreover, the serological identification of salmonella spp. was also done, using antisera specific to their antigenic structures. counts of bacteria in various sewage sludges and effluents tested showed the efficiency of the activated sludge process in reducing ... | 1982 | 7168261 |
| fever, antipyretics and serum iron levels in rabbits. | the intracerebroventricular (i.c.v.) injection of bacterial endotoxin (shigella dysenteriae) produces a rise in deep-body temperature in rabbits. the increase in body temperature was partially inhibited by polymyxin b (p), and completely suppressed by ketoprofen (k). the systemic injection of bacterial endotoxins (shigella dysenteriae or salmonella typhosa) elicited an elevation of body temperature in rabbits which was partially blocked by polymyxin b and completely antagonized by ketoprofen. se ... | 1982 | 6983446 |
| ventricular fibrillation during acute rejection after heart transplantation. | the case of a 46-year-old patient who underwent orthotopic heart transplantation for treatment of end-stage heart failure as a result of ischemic heart disease is reported. four months after transplantation a grade ii rejection episode was complicated by ventricular fibrillation. lidocaine and procainamide intravenously did not effectively prevent recurrence. an increase of antirejection therapy in combination with flecainide acetate effectively prevented further episodes of ventricular fibrilla ... | 1982 | 1498146 |
| are our dental laws adequate? | 1982 | 351168 | |
| [dyslipidemia and accelerated atherosclerosis in chronic uremia: a probably decreasing pathology, due to nutritional studies and better dietary guidelines]. | 1982 | 216602 | |
| opening pandora's box. | 1983 | 264908 | |
| clinical and structural features and possible pathogenic mechanisms of dental fluorosis. | 1983 | 272716 | |
| radiotherapy in leukemia and lymphoma of children. | radiotherapy is important in the treatment of leukemia and lymphoma of children. in acute lymphocytic leukemia administration of cranial irradiation early during chemotherapy-induced remission prevents initial meningeal relapse. when cranial irradiation is combined with a 3-year course of multiple drug systemic chemotherapy approximately one-half of the children remain in complete remission for 5 years or more and are at little risk of relapse. preventive cranial irradiation is effective in chil ... | 1983 | 264798 |
| laws, regulations, and policies. | 1983 | 347936 | |
| health manpower in new zealand. the swinging pendulum. | 1983 | 272578 | |
| [theoretical conformation analysis of specific o-antigenic polysaccharides. polysaccharide of shigella dysenteriae of serotype 2]. | theoretical conformational analysis of shigella dysenteriae serotype 2 polysaccharide containing repeating unit: -4gal beta 1-3galnac alpha 1-3(glcnac alpha 1-4)galnac alpha 1-4glc alpha 1- has been carried out. it is shown that spatial forms of the polymer are determined by conformational states of glc alpha 1-4gal links which form the internal bends of the chain. the structures of low energy are shown to be left three-fold helices with an axially projected repeat of approximately 12 a. n-aceta ... | 1983 | 6207841 |
| hospital-associated outbreak of shigella dysenteriae type 2--maryland. | 1983 | 6405168 | |
| shigella dysenteriae bacteremia. | 1983 | 6889165 | |
| [shigellosis abroad]. | 1983 | 6379992 | |
| shigella dysenteriae 1 cytotoxin: periplasmic protein releasable by polymyxin b and osmotic shock. | treatment of shigella dysenteriae 1 either with the antibiotic polymyxin b or by osmotic shock resulted in the release of 80 to 90% of the cytotoxin activity of the organism. under the conditions employed, the release of toxin activity was accompanied by the appearance of a periplasmic enzyme, 5'-nucleotidase. there was no significant release of cytoplasmic contents, assessed by measurement of glucose-6-phosphate dehydrogenase activity. the release of cytotoxin and 5'-nucleotidase by polymyxin b ... | 1983 | 6295958 |
| release of shiga toxin from shigella dysenteriae 1 by polymyxin b. | release of shiga toxin from shigella dysenteriae 1 was found to occur after exposure to polymyxin b. the amount of toxin released was dependent on both the polymyxin concentration and time of incubation. an immunoblot characterization of the shiga toxin released by polymyxin treatment demonstrated that it is electrophoretically similar to purified shiga toxin and to shiga toxin present in crude bacterial sonicates of s. dysenteriae 1 cells. | 1983 | 6299964 |
| peripheral neuropathy following shigella dysentery. | 1983 | 6316568 | |
| [diagnostic test system for the quantitative determination of shigella antigens in patient blood by an immunoenzyme analytical method]. | the diagnostic test system under trial has been shown to permit the detection of s. sonnei and s. flexneri specific antigens with an accuracy of 10(-3) micrograms. along with high sensitivity, the test system has sufficiently high specificity. statistically significant differences in the occurrence of specific dysentery antigens and their levels in the blood of dysentery patients and healthy persons have been revealed. | 1983 | 6362300 |
| studies of enterotoxin of shigella dysenteriae type 1. i. effect of aeration on the production of enterotoxin and suitability of laboratory animals for its biological assay. | shigella dysenteriae type 1 was used to study the influence of aeration on enterotoxin production and the suitability of laboratory animals for the toxin assay. the enterotoxin produced under aerobic shake culture conditions gave a gut dilatory response of 1.436 but that obtained in aerobic and anaerobic stationary cultures exerted no activity. the toxin fractionated on sephadex g-150 column was more effective than that obtained by ammonium sulphate precipitation. only sephadex column fraction i ... | 1983 | 6362316 |
| immunochemical studies on a polysaccharide from shigella dysenteriae type 2. | the polysaccharide isolated from shigella dysenteriae type 2, strain nctc 566, on smith degradation and graded hydrolysis yielded three oligosaccharides which were characterised using methylation studies. using homologous rabbit antiserum and the monosaccharides that constituted the polysaccharide and the oligosaccharides isolated from it and the cross-reactions in some type-specific pneumococcal antisera, immunochemical specificities of different sugar groupings in the polysaccharide molecule w ... | 1983 | 6371498 |
| [transfer of h2s plasmid from e. coli to shigella]. | 1983 | 6374333 | |
| animal toxicity of shigella dysenteriae cytotoxin: evidence that the neurotoxic, enterotoxic, and cytotoxic activities are due to one toxin. | the lethal effect to rabbits and mice of shigella dysenteriae toxin and the ability of the toxin to induce fluid accumulation in rabbit ileal loops were studied in relation to the cytotoxic activity. the relative concentrations of the three activities were approximately the same in a crude toxin preparation and in purified, electrophoretically homogenous toxin. the cytotoxic and lethal activities eluted identically from a high pressure liquid chromatography column and migrated at the same rate i ... | 1983 | 6336624 |
| rapid method for detection, identification, and susceptibility testing of enteric pathogens. | three hundred and seven colonies believed to be enteric pathogens were selected from primary plates of macconkey, xylose desoxycholate, or salmonella-shigella agar for inoculation to lactose-sucrose broth, urea-41 motility medium, modified andrade glucose broth with inverted durham tube, pregrowth broth, triple sugar iron agar, lysine iron agar (lia), and christensen urea agar. the rapid screen consisted of interpreting the lactose-sucrose, urea-41 motility, and modified andrade glucose broth ga ... | 1983 | 6338037 |
| shigellosis with bacteremia: a report of two cases and a review of the literature. | it is widely believed by both physicians and microbiologists that bacteremia is a rare event in shigellosis. we report two cases of shigellosis with positive blood cultures and review 68 cases reported in the literature between 1963 and 1981. we suggest that detection of bacteremia in shigellosis may be facilitated by a greater awareness of the following findings. 1. eighty-seven percent of all cases of shigellosis with bacteremia reported in the literature during the 18-year period reviewed occ ... | 1983 | 6340078 |
| purification and characterization of a shigella dysenteriae 1-like toxin produced by escherichia coli. | a toxin from an enteropathogenic strain of escherichia coli (e. coli h30) was purified to apparent homogeneity from cell lysates. the steps used to isolate the e. coli h30 toxin included french pressure-cell disruption of bacteria grown in iron-depleted media. affi-gel blue chromatography, chromatofocusing, and anti-shiga toxin affinity chromatography. the mobilities of the subunits of radioiodinated e. coli h30 toxin and shiga toxin observed after the two toxins were subjected to sodium dodecyl ... | 1983 | 6341244 |
| shigella infections in the united states, 1974-1980. | during the seven-year period 1974-1980, 93,516 shigella isolates from humans were reported to the centers for disease control, atlanta, through a nationwide surveillance system. over the past 30 years, the reported incidence of shigellosis has been declining in contrast to that reported for salmonellosis. shigella sonnei (group d) now accounts for approximately 70% of the shigella isolates reported, since most of the decline has been due to the increasingly less frequent infections caused by shi ... | 1983 | 6341480 |
| [epidemiology of bacillary dysentery in algeria. i. the epidemiological aspects of dysentery in algeria]. | bacterial dysentery plays an important role among infectious diseases in algeria. a tendency to the growth of the morbidity rate has been observed. dysentery is irregularly spread in different zones of the country. the highest morbidity rate is registered in the sahara zone and in the eastern part of the country. the morbidity rate among the urban population is higher than among the rural population. s. flexneri prevail in the etiological structure of dysentery infection. of all age groups, the ... | 1983 | 6342313 |
| leads from the mmwr. shigella dysenteriae type 2 outbreak in us naval hospital. | 1983 | 6343642 | |
| multiple enteric pathogens in travelers from endemic areas: case report. | 1983 | 6344046 | |
| [eternal return... the reappearance of shiga bacillus in central africa]. | 1983 | 6344812 | |
| outbreak of shigella dysentery in eastern zaire, 1980-1982. | 1983 | 6344814 | |
| isolation and characterization of monoclonal antibodies to shiga toxin. | hybridoma cell lines which produce monoclonal antibodies to shiga toxin from shigella dysenteriae 1 were prepared. the monoclonal antibodies were all of the immunoglobulin g1 isotype and differed in their ability to neutralize cytotoxicity and to bind to shiga toxin in a solid-phase radioimmunoassay. when used for immunoblot analysis, these antibodies were able to identify specifically both nicked and unnicked shiga toxin in crude lysates of s. dysenteriae. | 1983 | 6345399 |
| [new directions in the study of immunity in dysentery]. | 1983 | 6346759 | |
| [causes of sequential changes in the etiological structure of dysentery and the other epidemiologic patterns of this infection]. | 1983 | 6349187 | |
| trimethoprim-sulphamethoxazole-resistant shigella dysenteriae serotype 1 (shiga's bacillus) in gimira, south-west ethiopia. | 1983 | 6354711 | |
| pathogenesis of shigella diarrhea. viii. evidence for a translocation step in the cytotoxic action of shiga toxin. | shigella toxin interacts with hela cells by binding to a glycoprotein cell surface receptor and, after a lag period, inactivates the 60s ribosomal subunit and inhibits protein synthesis. the lag period may be due to energy-dependent translocation of the toxin to the interior of the cells. this step was investigated with agents known to inhibit glucose metabolism, mitochondrial energy production, or macromolecular synthesis and with drugs that alter the cytoskeletal system or lysosomes. metabolic ... | 1983 | 6355319 |
| [topology of the structural proteins of long tail fibers of phages t4d, ddvih+ and ddvih]. | topology of the products of the genes 34, 35, 36 and 37 of the bacteriophage t4d long tail fibers were determined with the aid of monospecific antibodies. the antibodies against gene product 34 were the only to interact with the proximal part of long tail fibers, but the distal part bound the antibodies against 35, 36 and 37. product of the gene 35 is located at the joint-site with the distal part and binds the distance not more than 75 a long. gene product 36 is located between these of 35 and ... | 1983 | 6355821 |
| [trends in the spread of boyd 4 dysentery and the characteristics of its clinical picture and of the biology of its causative agent]. | the data on the proportion of s. boydii 4 in the general structure of the causative agents of dysentery, as well as in the intraspecific structure of s. boydii, in some areas of the ussr in 1977-1981 are presented. s. boydii strains 4, circulating in one of the areas where their proportion considerably increased in 1980-1981, corresponded to their taxonomic position in their biological properties, while forming a single biochemical variant according to the character of the fermentation of sorbit ... | 1983 | 6356722 |
| molecular comparison of virulence plasmids in shigella and enteroinvasive escherichia coli. | virulent isolates of shigella dysenteriae and shigella boydii harboured a 140 mdal plasmid which was either absent or deleted in spontaneously avirulent strains. together with previous data concerning s. sonnei, s. flexneri and enteroinvasive escherichia coli, the present results established the general role of extrachromosomal elements in the virulence of such enteroinvasive species. among different species, these virulence plasmids showed unrelated endonuclease cleavage patterns, whereas hybri ... | 1983 | 6357023 |
| entry of shigella dysenteriae toxin into hela cells. | the rate of shigella toxin entry into the cytosol of hela s3 cells was estimated from the toxin-induced reduction in protein synthesis. whereas high toxin concentrations strongly reduced protein synthesis within 30 min, lower concentrations required longer times. the major part of the cell-bound toxin entered only after several hours. toxin entered cells after incubation at 25 degrees c but not at 20 degrees c, although toxin binding was the same at the two temperatures. increasing the kcl conce ... | 1983 | 6358031 |
| immunochemical studies on shigella dysenteriae type 10 bacterial polysaccharide. | on graded hydrolysis and smith degradation, the o-somatic polysaccharide isolated from shigella dysenteriae type 10 bacteria yielded four oligosaccharides which were characterized by methylation studies. immunochemical studies using constituent monosaccharides and the oligosaccharides indicated that the sugar grouping leads to 3)-mannac-(1 leads to 3)-rha-(1 leads to 4)-glcnac-(1 leads to was the immunodominant part in the polysaccharide molecule, and that 2-acetamido-2-deoxy-d-mannose contribut ... | 1983 | 6200225 |
| immunochemical studies on shigella dysenteriae type 9 bacterial polysaccharide. | on graded hydrolysis and smith degradation, the o-somatic polysaccharide isolated from shigella dysenteriae type 9 bacteria, strain nctc 7919, yielded five oligosaccharides which were characterized. the positions of the o-acetyl and pyruvic acetal groups in the repeating unit were identified. immunochemical studies indicated that d-galactose is the immunodominant sugar in the polysaccharide, and one of the oligomers, having the structure gal-(1 leads to 3)-glcnac-(1 leads to 3)-gal-(1 leads to 4 ... | 1983 | 6189606 |
| antigenic relationships between the enteroinvasive escherichia coli o antigens o28ac, o112ac, o124, o136, o143, o144, o152, and o164 and shigella o antigens. | enteroinvasive escherichia coli strains have been found in the following o serogroups: o28ac, o112ac, o124, o136, o143, o144, o152, and o164. the biochemical reactions of these enteroinvasive e. coli strains are often similar to those of shigella strains, and identification may be difficult. the problem of differentiation and identification is further complicated by the sharing of antigenic components. the antigenic relationships between these o antigens and those of all the established and prov ... | 1983 | 6189854 |
| resistant shigella dysenteriae. | 1983 | 6140517 | |
| escherichia coli o157:h7 strains associated with haemorrhagic colitis in the united states produce a shigella dysenteriae 1 (shiga) like cytotoxin. | 1983 | 6132054 | |
| cholera-like enterotoxin produced by campylobacter jejuni. characterisation and clinical significance. | the presence and clinical significance of enterotoxins produced by campylobacter jejuni were investigated. the supernatant of a prototype virulent strain grown in supplemented medium induced intraluminal fluid secretion in rat ileal loop but not in rabbit ileal loop or the infant mouse assay. it induced elongation and increased intracellular cyclic amp levels in chinese hamster ovary cells. toxin activity was blocked by cholera antitoxin and was destroyed by heat and high or low ph; its molecula ... | 1983 | 6135079 |
| purification of shigella dysenteriae 1 (shiga)-like toxin from escherichia coli o157:h7 strain associated with haemorrhagic colitis. | 1983 | 6136724 | |
| co-trimoxazole-resistant shigella dysenteriae type 1 outbreak in a family in bangladesh. | 1983 | 6137632 | |
| environmental and human isolates of vibrio cholerae and vibrio parahaemolyticus produce a shigella dysenteriae 1 (shiga)-like cytotoxin. | some strains of vibrio parahaemolyticus, vibrio cholerae, and non-o1 v cholerae produce a bacterial-cell-associated, heat-stable material that is cytotoxic for hela cells. cytotoxicity is completely neutralised by antibody to purified shigella dysenteriae 1 (shiga) toxin but not by antibody to purified cholera toxin. | 1984 | 6140426 |
| epidemic bacillary dysentery in west bengal, india, 1984. | 1984 | 6145892 | |
| nalidixic acid in shigella dysenteriae outbreaks. | 1984 | 6146074 | |
| shiga bacillus: west bengal to bristol. | 1984 | 6146826 | |
| epidemic of dysentery in west bengal: clinicians' enigma. | 1984 | 6150220 | |
| [neutralizing action of human blood preparations on shigella enterotoxins and choleragen]. | the possibility of neutralizing the enterotoxic activity of s. dysenteriae 1 neurotoxin, s. sonnei live virulent cultures and cholerigen with immune sera of different animals, normal human sera and commercial gamma-globulin preparations is shown. | 1984 | 6205524 |
| immunoblotting procedure for the analysis of electrophoretically-fractionated bacterial lipopolysaccharide. | a procedure is described for the efficient transfer of fractionated bacterial lipopolysaccharide (lps) from sds-polyacrylamide gels to nitrocellulose filters, and its subsequent display by a peroxidase-linked antibody. the method is sensitive, and reveals and resolves high molecular weight lps molecules having side chain lengths of up to and greater than 30 repeat units. it is useful for the rapid analysis of lps in bacterial outer membrane preparations. | 1984 | 6084988 |
| temperature-dependent expression of virulence genes in shigella species. | the pathogenicity of shigella spp. involves the ability of the bacteria to penetrate and replicate within the epithelial cells of the large intestine. model systems for examining the virulence of shigellae employ henle intestinal epithelial cells in tissue culture and an in vivo assay for virulence in guinea pig eyes (sereny test). using these systems, we studied the genetic and physiological bases for the ability of shigellae to invade epithelial cells. we found that expression of virulence in ... | 1984 | 6360895 |
| a small plasmid in shigella dysenteriae 1 specifies one or more functions essential for o antigen production and bacterial virulence. | the role of plasmids in the virulence of shigella dysenteriae 1 w30864, which contains at least five species, was investigated. by means of a standard plasmid-curing procedure, that is, bacterial cultivation at an elevated temperature, five virulence-deficient derivatives were obtained. one of these lacked a small, 6-megadalton plasmid, designated phw400, exhibited reduced invasiveness for hela cells, and failed to produce the somatic antigen. transposon tagging of the phw400 plasmid to produce ... | 1984 | 6360905 |
| [recent advances in the molecular mechanism of action of bacterial toxins, in particular of diphtheria, cholera, coli, botulinum and shigella toxins as well as tetanospasmin and the toxins of staphylococcus aureus]. | great progress was achieved in the clarification of the molecular structure and the mechanism of action of the toxins of pathogenic forms of bacteria. proportions of toxins of corynebacterium diphtheriae and of pseudomonas aeruginosa transfer from the nad and adp-ribose protein to an amino acid of the elongation factor 2. thus the protein synthesis is much inhibited. the cholera toxin and the l-toxin from escherichia coli have a similar structure. they transfer an adp-ribose portion from nad to ... | 1984 | 6375175 |
| dried filter paper for sending dysenteric faeces to the laboratory. a neglected method? | 1984 | 6375592 | |
| circulating antisomatic antibody response in shiga dysentery & igm & igg activities after 2-mercaptoethanol treatment. | 1984 | 6378776 | |
| [arthritis in shigella and salmonella infections]. | 1984 | 6379799 | |
| purified shigella enterotoxin does not alter intestinal motility. | a purified shigella enterotoxin (pst) and a cell-free lysate with pst removed (cfl-pst) from the whole-cell lysate of shigella dysenteriae 60 r were used to study their effect on the myoelectric activity and mucosal integrity of rabbit ileal segments. we have previously defined two myoelectric patterns: the migrating action potential complex and repetitive bursts of action potentials that occur in response to certain bacteria and their enterotoxins. the in vivo model consisted of isolated ileal ... | 1984 | 6363286 |
| shiga toxin: no smoking gun. | 1984 | 6364352 | |
| r plasmids conferring multiple drug resistance from shigella isolated in korea. | the majority (85%) of shigella isolated in 1980 and 1981 in korea were shigella flexneri, the others were sh. sonnei (14%) with only a small number of sh. dysenteriae. only 14 of the 459 strains of shigella isolated were susceptible to all 12 drugs tested, and 445 were resistant to three or more drugs. strains multiply resistant to the six drugs, chloramphenicol (cm), tetracycline (tc), streptomycin (sm), sulfisomidine (su), ampicillin (ap) and trimethoprim (tp) were most frequently encountered, ... | 1984 | 6368684 |
| shigella dysentery in tripoli, libya. | 1984 | 6371260 | |
| comparative study of the nature and biological activities of bacterial enterotoxins. | it is apparent that there are considerable similarities between many of the enterotoxins produced by enteric pathogens. although the effect of most of these toxins is restricted to the intestine in vivo, many cells are also sensitive to intoxication in vitro. the resultant in-vitro biochemical changes may have no pathological significance but serve to underline the central role of cyclic nucleotides in cellular fluid regulation. the biological activity of these enterotoxins is the result of inte ... | 1984 | 6327986 |
| comparative efficacies of pivmecillinam and ampicillin in acute shigellosis. | the clinical efficacies of pivmecillinam and ampicillin were compared in a randomized double-blind trial in the treatment of acute shigellosis. of 44 adult male patients, all culture positive for shigella strains, 22 patients received 400 mg of pivmecillinam and 22 patients received 500 mg of ampicillin every 6 h. both drugs were administered orally for 5 days. four patients receiving ampicillin were infected with shigella strains that were resistant to ampicillin but susceptible to pivmecillina ... | 1984 | 6329092 |
| changes in the trend of shigellosis in dhaka: family study on secondary infection, clinical manifestation and sensitivity pattern: 1980. | the incidence of shigellosis and the death rate have increased and the resistance of shigellae to antibiotics has changed in dhaka during our experiences. in 1980, we investigated the secondary infection and case rates, infection to case ratio, duration of illness, excretion of shigellae and antibiotic sensitivity pattern in 100 families with cases of shigellosis, culturing rectal swabs obtained by home visits for a 10-day period. standard methods were used for culture and sensitivity tests. the ... | 1984 | 6380011 |
| effect of bacterial endotoxin on body temperature, plasma zinc and plasma concentrations of the acute-phase protein serum amyloid p component in mice. | bacterial endotoxin and lipid a evoked dose-dependent increases in body temperature and plasma sap concentrations and dose-dependent falls in plasma zinc concentrations in mice. the respective sensitivities of the three variables to lipid a or to whole endotoxin had the relation sap greater than zinc much greater than body temperature; zinc and sap responses were evoked by less than 1 ng lipid a/endotoxin. pretreatment with indomethacin prevented only the temperature response to lipid a. pretrea ... | 1984 | 6380557 |
| small virulence plasmid of shigella dysenteriae 1 strain w30864 encodes a 41,000-dalton protein involved in formation of specific lipopolysaccharide side chains of serotype 1 isolates. | a 6-megadalton plasmid, phw400, of shigella dysenteriae 1 strain w30864 was previously found to specify one or more functions for o-antigen production and bacterial virulence (h. watanabe and k. n. timmis, infect. immun. 43:391-396, 1984). the region of phw401, a tn801-tagged derivative of phw400, responsible for o-antigen production has been localized by gene cloning and tn5 transposon mutagenesis. analysis of lipopolysaccharide isolated from s. dysenteriae 1 bacteria carrying mutant plasmids r ... | 1984 | 6384048 |
| [a patient with a complicated course of shigellosis]. | 1984 | 6384800 | |
| the humoral antibody response to shigella dysenteriae type 1 infection, as determined by elisa. | an enzyme-linked immunosorbent assay (elisa) for determining the class-specific humoral antibody response to the lipopolysaccharide antigen from shigella dysenteriae serotype 1 bacteria has been tested. two or more serum samples from each of 60 persons infected with this organism during a dysentery outbreak in a boarding school for young men near haiphong, viet nam, and single serum samples from 39 healthy vietnamese and from 20 healthy swedes were included in the study. comparison of the titres ... | 1984 | 6386207 |
| usefulness of plasmid profiles for differentiation of shigella isolates in bangladesh. | we studied the plasmid profiles of 136 shigella isolates in bangladesh to determine whether plasmid profiles could be used for differentiation of strains for epidemiological studies. many different plasmid patterns were observed within each species, indicating that many genetically different strains of shigella are responsible for illness in bangladesh. | 1984 | 6386850 |
| expression of lipopolysaccharide o antigen in escherichia coli k-12 hybrids containing plasmid and chromosomal genes from shigella dysenteriae 1. | the requirement for both plasmid and chromosomal genes in the biosynthesis of shigella dysenteriae 1 lipopolysaccharide o antigen was demonstrated in escherichia coli-shigella hybrids. a 6-megadalton s. dysenteriae 1 plasmid, designated pwr23, was phenotypically tagged with the tn3 ampicillin-resistance transposon. the tagged plasmid, designated pwr24, was transferred by transformation or conjugal mobilization to a rough e. coli k-12 recipient. although the resultant hybrids were agglutinated in ... | 1984 | 6389345 |
| epidemic dysentery caused by the shiga bacillus in a southern indian village. | multi-drug resistant shigella dysenteriae type 1 caused an epidemic of dysentery in a village in southern india. the epidemic started as a common source outbreak, through the piped water supply of the village with subsequent person-to-person spread. although the attack rate was high, with nearly 1/2 the children under age 5 being affected, the case fatality rate was only about 1%. of the 248 patients who attended the field clinic, 89.4% passed small mucoid stools mixed with blood, with the ma ... | 1984 | 6389664 |
| [bacillary dysentery 1982]. | 1984 | 6390523 | |
| epidemic shiga bacillus dysentery in central africa. | an outbreak of dysentery began late in 1979 in central africa and spread to involve a major portion of zaire as well as rwanda and burundi. we traveled to a mission hospital in northeast zaire during the epidemic and isolated shigella dysenteriae, type 1, from most of the patients studied. all isolates were resistant to ampicillin, tetracycline, chloramphenicol, sulfathiazole, and streptomycin but sensitive to trimethoprim-sulfamethoxazole. antimicrobial resistance was transferable to escherichi ... | 1984 | 6391226 |
| [molecular structure of the o-specific polysaccharide of shigella dysenteriae type 2]. | 1984 | 6391882 | |
| pathogenesis of shigella diarrhea. ix. simplified high yield purification of shigella toxin and characterization of subunit composition and function by the use of subunit-specific monoclonal and polyclonal antibodies. | a simple purification scheme for shigella cytotoxin was devised, resulting in high yields (approximately 50%) and a 1,300-fold increase in specific activity compared with the initial crude bacterial cell lysate. the purified toxin was enterotoxic in ligated rabbit ileal loops and neurotoxic when injected into the peritoneal cavity of mice. measurement of specific activity of cytotoxin and enterotoxin demonstrated that these two toxicities copurify during the fractionation procedure. on sodium do ... | 1984 | 6392471 |
| [the local epidemic of shigella dysenteriae in wuwei county, gansu province]. | 1984 | 6394140 | |
| shigellemia. | 1984 | 6394497 | |
| [shigella dysenteriae in our environment]. | 1984 | 6394922 | |
| shigellosis: an old disease with new faces. | 1984 | 6397507 |