Publications

the biologic significance of the mixed lymphocyte kidney culture in humans.	the mixed lymphocyte kidney culture (mlkc) in humans has been studied in normal and abnormal clinical conditions. human renal cortical cells were extracted by collagenase treatment from the kidneys of "normal" heart-beating cadaver organ donors (n = 13), patients with end-stage renal disease (esrd) at pretransplant bilateral nephrectomy and splenectomy (n = 13), and from irreversibly rejected renal allografts at the time of graft nephrectomy (n = 5). proliferation of peripheral blood t lymphocyt ...	1985	2996186
elicitation of conspecific attack or defense in the male rat by intraventricular injection of a gaba agonist or antagonist.	the involvement of central gabaergic mechanisms in the control over offensive and defensive behaviours in the rat was studied using intracerebroventricular injections (5 microliter) of a gaba agonist (thip) or a gaba antagonist (bicuculline methiodide). intracerebroventricular injections of thip (1.25 and 2.5 micrograms) induced attacks and offensive sideways towards an untreated partner, in animals placed in a neutral area where no aggressive reactions occur in controls. social approach behavio ...	1985	2999844
elicitation of conspecific attack or defense in the male rat by intraventricular injection of a gaba agonist or antagonist.	the involvement of central gabaergic mechanisms in the control over offensive and defensive behaviours in the rat was studied using intracerebroventricular injections (5 microliter) of a gaba agonist (thip) or a gaba antagonist (bicuculline methiodide). intracerebroventricular injections of thip (1.25 and 2.5 micrograms) induced attacks and offensive sideways towards an untreated partner, in animals placed in a neutral area where no aggressive reactions occur in controls. social approach behavio ...	1985	2999844
repression of adenovirus early gene expression by coinfection with a temperature-sensitive mutant in the immediate-early gene of pseudorabies virus.	wild-type adenovirus was coinfected with a mutant temperature sensitive for the immediate-early gene of pseudorabies virus. at the nonpermissive temperature, this mutant, tsg, strongly inhibited the transcription of all adenovirus early genes, including e1a. this inhibition was not observed with wild-type pseudorabies virus coinfection or with tsg coinfection at the permissive temperature. the level of repression was dependent upon the ratio of tsg to adenovirus in the infection. the results sug ...	1986	3001339
synaptic transmission in the rabbit inferior mesenteric ganglion.	electrical properties, cholinergic neurotransmission and non-cholinergic neurotransmission in the rabbit inferior mesenteric ganglion (img) in vitro were examined with intracellular recording techniques. a single ganglionic neuron received an average of 42 nicotinic cholinergic synaptic inputs. an atropine-sensitive slow excitatory postsynaptic potential not followed by a non-cholinergic late slow excitatory postsynaptic potential (ls-epsp) was observed in 7% of the cells. in 63% of the cells a ...	1985	3003179
blood concentration and urinary excretion of enalapril in patients with chronic renal failure.		2010	3003431
interaction of stable prostaglandin endoperoxide analogs u46619 and u44069 with human platelet membranes: coupling of receptors with high-affinity gtpase and adenylate cyclase.	it has been demonstrated using a membrane preparation of human platelets that stable analogs of pgh2, u46619 and u44069, control the activity of adenylate cyclase and a high-affinity hormone-sensitive gtpase. at 10(-8)-10(-6) m, the analogs inhibit the basal activity of adenylate cyclase by 20-25%. with a further rise in u46619 and u44069 concentrations up to 10(-5)-10(-4) m, the inhibition is abolished and adenylate cyclase activity is stimulated in a dose-dependent fashion. in the presence of ...	1985	3003958
[experimental basis for the use of collagenase preparations to prevent aging of the skin].		1985	3004061
meiotic recombination between two polymorphic restriction sites within the beta globin gene cluster.	analysis of beta globin gene haplotypes for prenatal diagnosis of beta thalassaemia has revealed a recombination event within the beta globin gene cluster. both a change in the avaii polymorphic site within the beta globin gene and a change in the phenotype of the beta globin gene were observed. paternity was established by the pedigree analysis of hypervariable 'minisatellite' dna polymorphisms and the most probable explanation of the recombination event is a crossover between the psi beta glob ...	2010	3005579
lateral diffusion of specific antibodies bound to lipid monolayers on alkylated substrates.	we have measured the lateral mobility of fluoresceinated monoclonal igg antibodies bound specifically to a spin label lipid hapten in phospholipid monolayers supported on alkylated silicon oxide surfaces. dimyristoyl phosphatidylcholine and dipalmitoyl phosphatidylcholine monolayers containing 5 mol% of the lipid hapten were transferred by conventional langmuir-blodgett techniques onto substrates alkylated with hydrocarbon chains containing 10, 16, and 18 carbon atoms. we show that the diffusion ...	2010	3006037
lateral diffusion of specific antibodies bound to lipid monolayers on alkylated substrates.	we have measured the lateral mobility of fluoresceinated monoclonal igg antibodies bound specifically to a spin label lipid hapten in phospholipid monolayers supported on alkylated silicon oxide surfaces. dimyristoyl phosphatidylcholine and dipalmitoyl phosphatidylcholine monolayers containing 5 mol% of the lipid hapten were transferred by conventional langmuir-blodgett techniques onto substrates alkylated with hydrocarbon chains containing 10, 16, and 18 carbon atoms. we show that the diffusion ...	2010	3006037
near infrared optical monitoring of intact skeletal muscle during hypoxia and hemorrhagic hypotension in cats.		1985	3008518
complete dissociation between the activation of phosphoinositide turnover and of nadph oxidase by formyl-methionyl-leucyl-phenylalanine in human neutrophils depleted of ca2+ and primed by subthreshold doses of phorbol 12,myristate 13,acetate.	evidences have been provided by many laboratories that the activation of the nadph oxidase in neutrophils by formyl-methionyl-leucyl-phenylalanine (fmlp) is strictly linked to a transduction pathway that involves the stimulation, via gtp binding protein, of the phosphoinositide turnover and the increase in [ca2+]i. the results presented in this paper demonstrate that fmlp can activate the nadph oxidase by triggering a transduction pathway completely independent of phosphoinositide turnover and c ...	1986	3008746
cloning and expression of the genes specifying shiga-like toxin production in escherichia coli h19.	some strains of escherichia coli produce a protein which is cytotoxic for vero cell and hela cell monolayers. this toxin is very similar to the toxin of shigella dysenteriae 1 and has been named verotoxin or e. coli shiga-like toxin. it has been shown that toxin conversion is due to a group of bacteriophages, one of which has been designated h-19b. in this study we report hybridization experiments showing that part of the h-19b genome is homologous to phage lambda. we have cloned a 1.7-kilobase ...	1986	3009393
[hypothesis on the probable mechanism of auto-limitation of acute attacks of gout].		2006	3013255
[response of polymorphonuclear neutrophils to monosodium urate crystals: production of oxygen free radicals].		2006	3013256
studies on enterotoxin of shigella dysenteriae type 1. ii. systemic effects in rabbits of shigella dysenteriae 1 enterotoxin.	the enterotoxin of shigella dysenteriae type 1 is an acid and heat-labile protein. it induces a gut dilatory response and increases the levels of blood glucose, serum alkaline phosphatase and serum acid phosphatase in rabbits.	1986	3019069
the effects of furosemide on adenosine 3':5'-cyclic monophosphate, guanosine 3':5'-cyclic monophosphate and corticosterone production stimulated by adrenocorticotropin in monolayer cultured rat adrenal cells.	furosemide has been reported to have a suppressive effect on adh-, pth- and adrenaline-stimulated adenosine 3':5'-cyclic monophosphate (camp) production, but the effect on adrenocorticotropin (acth) action has not yet been elucidated. in the present study, therefore, the effects of furosemide on camp and also on guanosine 3':5'-cyclic monophosphate (cgmp) and corticosterone, stimulated by acth in monolayer cultured rat adrenal cells, were investigated. the intra- and extracellular camp stimulate ...	1986	3019648
the effects of furosemide on adenosine 3':5'-cyclic monophosphate, guanosine 3':5'-cyclic monophosphate and corticosterone production stimulated by adrenocorticotropin in monolayer cultured rat adrenal cells.	furosemide has been reported to have a suppressive effect on adh-, pth- and adrenaline-stimulated adenosine 3':5'-cyclic monophosphate (camp) production, but the effect on adrenocorticotropin (acth) action has not yet been elucidated. in the present study, therefore, the effects of furosemide on camp and also on guanosine 3':5'-cyclic monophosphate (cgmp) and corticosterone, stimulated by acth in monolayer cultured rat adrenal cells, were investigated. the intra- and extracellular camp stimulate ...	1986	3019648
substrate phosphoprotein availability regulates eclosion hormone sensitivity in an insect cns.	the final step in the moulting of all insects is ecdysis, the shedding of the cuticle of the previous instar, which is triggered in lepidoptera by the neurosecretory peptide eclosion hormone. this hormone acts directly on the nervous system to release the appropriate motor patterns for larval, pupal and adult ecdysis, but there are only brief periods near the end of each moult when the nervous system is competent to respond to the hormone. previous experiments have shown that the action of eclos ...	1986	3020427
substrate phosphoprotein availability regulates eclosion hormone sensitivity in an insect cns.	the final step in the moulting of all insects is ecdysis, the shedding of the cuticle of the previous instar, which is triggered in lepidoptera by the neurosecretory peptide eclosion hormone. this hormone acts directly on the nervous system to release the appropriate motor patterns for larval, pupal and adult ecdysis, but there are only brief periods near the end of each moult when the nervous system is competent to respond to the hormone. previous experiments have shown that the action of eclos ...	1986	3020427
differential effects of transforming growth factor type beta on the growth and function of adrenocortical cells in vitro.	transforming growth factor type beta (tgf-beta) suppresses basal as well as corticotropin (acth)-stimulated steroid formation by bovine adrenocortical cells in culture. the effect is dose dependent and is not accompanied by any change in adrenocortical cell growth. the minimum effective dose of tgf-beta is 4 x 10(-13) m (10 pg/ml), and maximal inhibition is observed at a concentration of 4 x 10(-11) m (1 ng/ml). a 16- to 20-hr incubation with tgf-beta is required to decrease steroidogenesis, and ...	1986	3020557
differential effects of transforming growth factor type beta on the growth and function of adrenocortical cells in vitro.	transforming growth factor type beta (tgf-beta) suppresses basal as well as corticotropin (acth)-stimulated steroid formation by bovine adrenocortical cells in culture. the effect is dose dependent and is not accompanied by any change in adrenocortical cell growth. the minimum effective dose of tgf-beta is 4 x 10(-13) m (10 pg/ml), and maximal inhibition is observed at a concentration of 4 x 10(-11) m (1 ng/ml). a 16- to 20-hr incubation with tgf-beta is required to decrease steroidogenesis, and ...	1986	3020557
evidence that stimulation of growth hormone release by epinephrine and vasoactive intestinal peptide is based on cell-to-cell communication in the pituitary.	epinephrine (epi) evoked a strong concentration-dependent (1-1000 nm) rise of gh release from perifused rat anterior pituitary cells cultured as aggregates in a serum-free defined culture medium. dexamethasone (dex), added to the culture medium, enhanced the secretory response to epi. aggregates of pituitary cells separated by gradient sedimentation at unit gravity widely differed in responsiveness to epi, provided dex was added to the culture medium. the poorest response was seen in aggregates ...	1987	3023029
evidence that stimulation of growth hormone release by epinephrine and vasoactive intestinal peptide is based on cell-to-cell communication in the pituitary.	epinephrine (epi) evoked a strong concentration-dependent (1-1000 nm) rise of gh release from perifused rat anterior pituitary cells cultured as aggregates in a serum-free defined culture medium. dexamethasone (dex), added to the culture medium, enhanced the secretory response to epi. aggregates of pituitary cells separated by gradient sedimentation at unit gravity widely differed in responsiveness to epi, provided dex was added to the culture medium. the poorest response was seen in aggregates ...	1987	3023029
total iridectomy does not alter outflow facility responses to cyclic amp in cynomolgus monkeys.	total outflow facility was measured by two-level constant pressure anterior chamber perfusion in surgically untouched and totally iridectomized cynomolgus monkey eyes receiving bolus intracameral infusions of cyclic amp, dibutyryl cyclic amp, or 5'amp. cyclic amp doses of 50 and 100 micrograms (corresponding to initial intracameral concentrations of 1.5- and 3 mm) increased facility by approximately 20 and 40% respectively in both surgically untouched and aniridic eyes. higher doses produced no ...	1986	3023122
relative activities of 5'-nucleotidase and adenosine deaminase in atrial and ventricular myocardium--the enzyme paradox.	the 5'-nucleotidase and adenosine deaminase activities were determined for tissue extracts of up to 10 defined regions of the normal dog heart. the specific activity of 5'-nucleotidase was very significantly higher in atrial than in ventricular myocardium (4 x) and was also higher in right than in left sites of the heart (2 x). the reason for the markedly higher specific activity in atrial muscle is not clear and appears paradoxical relative to adenine nucleotide content and pattern of atp decay ...	1986	3025011
diacylglycerol lipase and kinase activities in rabbit aorta and coronary microvessels.	diacylglycerol lipase and kinase activities were measured in particulate and soluble fractions from rabbit aorta (intima-media) and coronary microvessels. with rabbit aorta, the hydrolysis at the sn-1 position of 1-palmitoyl-2-oleoyl-sn-glycerol had a ph optimum of 5-6 and was greater than hydrolysis at the sn-2 position (ph optimum of 6.5). only the 2-monoacylglycerol accumulated during incubations at ph 5 and 6.5. these results are consistent with an ordered two-step reaction sequence where th ...	1986	3026422
diacylglycerol lipase and kinase activities in rabbit aorta and coronary microvessels.	diacylglycerol lipase and kinase activities were measured in particulate and soluble fractions from rabbit aorta (intima-media) and coronary microvessels. with rabbit aorta, the hydrolysis at the sn-1 position of 1-palmitoyl-2-oleoyl-sn-glycerol had a ph optimum of 5-6 and was greater than hydrolysis at the sn-2 position (ph optimum of 6.5). only the 2-monoacylglycerol accumulated during incubations at ph 5 and 6.5. these results are consistent with an ordered two-step reaction sequence where th ...	1986	3026422
molecular properties of f9 embryoglycan recognized by a unique antibody in sera from patients with germ cell tumors.	human antibody against an embryoglycan present on a mouse teratocarcinoma cell line f9 was found in sera from 16 of 29 patients with embryonal carcinoma, yolk sac tumor, immature teratoma, and choriocarcinoma of gonadal and extragonadal origins by farr assay. in contrast, none of the sera from patients (77 cases) with dysgerminoma, seminoma, germinoma, and mature teratoma or from patients (118 cases) with nongerm cell types of ovarian tumors contained this antibody. the antigenic embryoglycan wa ...	1987	3032420
detection of aspartate kinase in rat liver and its activation by ca++ and calmodulin.	the enzyme aspartate kinase (ec 2.7.2.4) has been detected in rat liver (animal tissue) for the first time. this enzyme, like the aspartate kinase from bacteria and plants is inhibited by lysine and threonine. further, the activity of the enzyme is stimulated over two fold by ca++ and calmodulin and inhibited by egta, a ca++ chelator and trifluoperazine, an anti-calmodulin compound.	1987	3034280
detection of aspartate kinase in rat liver and its activation by ca++ and calmodulin.	the enzyme aspartate kinase (ec 2.7.2.4) has been detected in rat liver (animal tissue) for the first time. this enzyme, like the aspartate kinase from bacteria and plants is inhibited by lysine and threonine. further, the activity of the enzyme is stimulated over two fold by ca++ and calmodulin and inhibited by egta, a ca++ chelator and trifluoperazine, an anti-calmodulin compound.	1987	3034280
effects of rice fiber on fecal weight, apparent digestibility of energy, nitrogen and fat, and degradation of neutral detergent fiber in young men.	the effects of rice fiber on fecal weight, transit time, frequency of defecations, digestibility of nutrients and blood status were investigated in 5 healthy young men. each of them consumed a brown rice diet and then a polished rice diet for 2 weeks respectively. both diets contained 1.2 g protein per kg body weight. the brown rice diet contained 2 times as much dietary fiber as the polished rice diet. when they consumed the brown rice diet, it showed an increase of fecal weight and decrease of ...	2010	3035151
detection of leishmania parasites by dna in situ hybridization with non-radioactive probes.	in situ hybridization techniques develop rapidly into diagnostic tools of considerable value for detection of viruses and bacteria. here we report the application of this technique for the detection of leishmania parasites. biotin-labelled total promastigote dna was hybridized to cultured leishmania parasites and to blood and impression smears of infected mice. in promastigotes kinetoplasts were strongly stained, nuclei somewhat more diffuse. in amastigotes both nuclear and kinetoplast dna hybri ...	1987	3035535
the hypothalamic-pituitary-adrenal axis in depression.		2010	3035885
[expressing plasmid vectors on the basis of escherichia coli beta-galactosidase gene fragments].	with the use of synthetic dna fragments, a set of new plasmid vectors has been obtained. the vectors provided high level expression of peptides and small proteins in e. coli as fusions with fragments of beta-galactosidase of various length. these vectors were used to achieve expression of a synthetic gene for a functionally active fragment of bacteriorhodopsin. the yields of hybrid proteins consisting of beta-galactosidase and bacteriorhodopsin fragments were in the range of 5-30% from the total ...	1987	3036168
etoposide combination therapy for small cell carcinoma of the lung.	sixty-three consecutive patients with small cell carcinoma of the lung were treated by six cycles at 3-week intervals of etoposide 120 mg/m2 i.v. on day 1 and orally on days 2-5, adriamycin 40 mg/m2 i.v. on day 1 and vincristine 1.4 mg/m2 i.v. on day 1. tumour bed irradiation was administered to patients with limited disease after chemotherapy. in limited-disease and extensive-disease patients the median survival was 12 and 6 months respectively. the 2-year survival rate (life table) in limited- ...	2011	3040288
localization of stx, a determinant essential for high-level production of shiga toxin by shigella dysenteriae serotype 1, near pyrf and generation of stx transposon mutants.	hfr strains of shigella dysenteriae serotype 1 were constructed by transient integration of an rp4 plasmid derivative carrying transposon tn501 into the shigella chromosome through tn501-mediated cointegration. the hfr strains were mated with escherichia coli k-12 recipients carrying various auxotrophic markers, and e. coli recombinants which had received prototrophic shigella genes were selected. some of the e. coli transconjugants produced high levels of a cytotoxin which was neutralized by bo ...	1987	3040592
leads from the mmwr. shigella dysenteriae type 1 in tourists to cancun, mexico.		1988	3043025
direct cytotoxic action of shiga toxin on human vascular endothelial cells.	to help explain a role of the shiga toxin family in hemorrhagic colitis and hemolytic-uremic syndrome in humans, it has been hypothesized that these toxins cause direct damage to the vascular endothelium. we now report that shiga toxin purified from shigella dysenteriae 1 does indeed have a direct cytotoxic effect on vascular endothelial cells in cultures. human umbilical vein endothelial cells (huvec) in confluent monolayers were reduced 50% by 10(-8) m shiga toxin after a lag period of 48 to 9 ...	1988	3044997
isolation of shiga toxin-resistant vero cells and their use for easy identification of the toxin.	shiga toxin-resistant vero cells were isolated by treatment of the cells with nitrosoguanidine. these mutant cells were not affected by shiga toxin at more than 1 microgram/ml, although the parent vero cells were sensitive to 25 pg of the toxin per ml. immunofluorescence studies showed that all the mutant cells had lost toxin-binding capacity. the cytotoxic activities of various bacterial cultures against the parent and mutant cells were compared. all samples from 10 strains of shigella dysenter ...	1988	3045003
cloning and sequencing of a shiga-like toxin type ii variant from escherichia coli strain responsible for edema disease of swine.	a shiga-like toxin type ii variant (slt-iiv) is produced by strains of escherichia coli responsible for edema disease of swine and is antigenically related to shiga-like toxin type ii (slt-ii) of enterohemorrhagic e. coli. however, slt-iiv is only active against vero cells, whereas slt-ii is active against both vero and hela cells. the structural genes for slt-iiv were cloned from e. coli s1191, and the nucleotide sequence was determined and compared with those of other members of the shiga toxi ...	1988	3045088
congo red binding and salt aggregation as indicators of virulence in shigella species.	smooth strains of shigella dysenteriae type 1, shigella flexneri, shigella boydii, and shigella sonnei which form pigmented colonies (pcr+) on congo red agar were virulent in the sereny test. smooth variants unable to bind congo red (pcr-) were avirulent. measurements of dye uptake from solution showed that s. dysenteriae type 1 bound the most dye, followed in order of uptake by s. flexneri, s. boydii, and s. sonnei. using the salt aggregation test (sat) to determine cell surface hydrophobicity, ...	1988	3045151
the primary structure of the operons coding for shigella dysenteriae toxin and temperature phage h30 shiga-like toxin.	nucleotide(nt) sequences were determined for the toxin (sht) operon present in the chromosome of shigella dysenteriae 1 and for the shiga-like toxin (slt) operon found in the lambdoid phage h30 genome. the coding sequences of the sht and slt genes differ in 4 nt with 1 nt change responsible for an amino acid replacement. the deduced amino acid sequence in the a chain of the toxins is highly homologous to that of the a chain of ricin, a plant toxin. sht-coding mrnas were detected by mapping the 5 ...	1988	3049254
dna probes to identify shiga-like toxin i- and ii-producing enteric bacterial pathogens isolated from patients with diarrhea in thailand.	when shigella species, escherichia coli, and five other bacterial enteric pathogens isolated from children with diarrhea in thailand were tested for hybridization under stringent conditions with probes for shiga-like toxins i and ii, only 30 shigella dysenteriae 1 hybridized with the shiga-like toxin i probe.	1988	3049664
deletion of the shiga toxin gene in a chlorate-resistant derivative of shigella dysenteriae type 1 that retains virulence.	we used a probe specific for detecting the structural-gene sequences of shiga toxin to analyze the genetic nature of toxin synthesis in mutant derivatives of shigella dysenteriae type 1. a chlorate-resistant (chl) mutant (725-78) of s. dysenteriae type 1 strain 3818t, which had retained virulence but had lost production of high levels of cytotoxic activity associated with shiga toxin synthesis, contained a complete deletion of the shiga toxin structural-gene sequences. these structural-gene sequ ...	1988	3049838
office evaluation and management of the shoulder impingement syndrome.	the shoulder impingement syndrome is the most common cause of shoulder pain. a complete office evaluation can be aided by a printed exam sheet. careful consideration of the differential diagnosis is important, often employing the xylocaine injection test. the impingement syndrome responds to conservative treatment the majority of the time. long follow-up determines the need for an invasive evaluation or surgical treatment.	2011	3050719
[differentiation of virulent strains of shigella and entero-invasive escherichia from their avirulent variants using modified indirect immunoenzyme analysis].	the data obtained in this investigation confirm that the modified indirect enzyme immunoassay (eia) permits the differentiation of virulent bacteria of the genus shigella and enteroinvasive escherichia (group 1), regularly containing virulence plasmids with a molecular weight of 120-140 md, from their avirulent variants which have lost these plasmids (group 2). the ratio of the optic density (od) values of the positive control samples (the od of group 1) to the od values of the negative ones (th ...	1988	3051808
the ecology and pathogenicity of urease-producing bacteria in the urinary tract.	urease activity is a physiological function of many bacteria that enables these organisms to utilize urea as a source of nitrogen. the association of ureolytic bacteria with human or animal hosts varies widely from a commensal relationship as demonstrated with skin microflora, a symbiotic relationship in the gastrointestinal tract, to a pathogenic relationship in the urinary tract. since similar or identical species of bacteria such as staphylococcus aureus are found in all three environments, t ...	1988	3053050
the ecology and pathogenicity of urease-producing bacteria in the urinary tract.	urease activity is a physiological function of many bacteria that enables these organisms to utilize urea as a source of nitrogen. the association of ureolytic bacteria with human or animal hosts varies widely from a commensal relationship as demonstrated with skin microflora, a symbiotic relationship in the gastrointestinal tract, to a pathogenic relationship in the urinary tract. since similar or identical species of bacteria such as staphylococcus aureus are found in all three environments, t ...	1988	3053050
development of dna probes for cytotoxin and enterotoxin genes in enteric bacteria.	dna probes to identify the genes encoding toxins in enteric bacteria have been developed. use of these probes reduces the number of animals required for toxicity testing, as suspect bacteria can be directly tested for the presence of toxin. we have augmented the gene probes available by developing probes against the escherichia coli enterotoxin ltii and shiga toxin from shigella dysenteriae 1. the ltii gene from e. coli 357900 was identified and characterised and a suitable internal probe was ob ...	1988	3053231
antibody response to lipopolysaccharide antigen in shigella dysenteriae type 1 infection.		1988	3053437
role of shiga toxin in the pathogenesis of bacillary dysentery, studied by using a tox- mutant of shigella dysenteriae 1.	a tox- mutant of shigella dysenteriae 1, sc501, was genetically engineered by cloning the shiga toxin operon, inserting a cassette into the a subunit gene, and exchanging this in vitro-mutagenized sequence with the wild-type gene. sc501 produced a low amount of residual cytotoxicity which was not neutralized by a rabbit immune serum directed against shiga toxin. invasion of cultured cells demonstrated that shiga toxin had no effect on the rate of intracellular growth of bacteria or on the rapid ...	1988	3053452
extraintestinal manifestations of shigellosis during an epidemic of bacillary dysentery in port blair, andaman & nicobar island (india).		1988	3053630
plasmid analysis of shigella dysenteriae type 1 isolates obtained from widely scattered geographical locations.	plasmid profiles and antimicrobial susceptibility patterns of 343 strains of shigella dysenteriae type 1, obtained from 18 different geographical locations, were analyzed. three plasmids, with molecular sizes of 140, 6, and 2 megadaltons (mda), were present in 94, 98, and 96%, respectively, of the 343 strains isolated during either epidemic or nonepidemic periods from 1965 to 1987. in addition to these plasmids, 83% of the strains harbored a 4-mda plasmid and 25% harbored a 20-mda plasmid. vario ...	1988	3053762
head posture and hyo-mandibular function in man. a synchronized electromyographic and videofluorographic study of the open-close-clench cycle.	synchronized electromyography and videofluorography (lateral projection) were used to investigate the influence of altered head posture on hyo-mandibular movements, suprahyoid muscle length, suprahyoid working angle, and timing of suprahyoid and masseter muscle activity. twelve adult male subjects with normal dentofacial morphology were investigated during the open-close-clench cycle. using upright head position (frankfort horizontal) as reference, several statistically significant observations ...	1988	3055938
head posture and hyo-mandibular function in man. a synchronized electromyographic and videofluorographic study of the open-close-clench cycle.	synchronized electromyography and videofluorography (lateral projection) were used to investigate the influence of altered head posture on hyo-mandibular movements, suprahyoid muscle length, suprahyoid working angle, and timing of suprahyoid and masseter muscle activity. twelve adult male subjects with normal dentofacial morphology were investigated during the open-close-clench cycle. using upright head position (frankfort horizontal) as reference, several statistically significant observations ...	1988	3055938
anticytotoxin-neutralizing antibodies in immune globulin preparations: potential use in hemolytic-uremic syndrome.	the pathogenesis of primary (classic) hemolytic-uremic syndrome (hus) is thought to be related to cytotoxin-producing enteric pathogens such as shigella dysenteriae serotype 1 and escherichia coli serotypes o157:h7 and 026:h11. the relevant cytotoxins include shiga toxin and the closely related shiga-like toxins (slts) produced by some e. coli strains. intravenously administered immune globulin (ivig) therapy has been reported to be beneficial in a few children with hus. we therefore examined co ...	1988	3057156
transferable or mobilisable antibiotic resistance in shigella dysenteriae types 1, 2, 3, 4, 6 and 7 isolated in ethiopia during 1974-85.	a total of 199 shigella dysenteriae isolates resistant to one or more antibiotics and belonging to types 1, 2, 3, 4, 6 and 7 was examined by one-step broth mating with escherichia coli k12 and, if non-conjugative, additionally by triparental crosses with the conjugative plasmids x and delta. of the s. dysenteriae type 1 (shiga's bacillus) strains, 96% harboured conjugative plasmids. during 1974-79, isolates of shiga's bacillus carried conjugative plasmids coding for acssut (ampicillin, chloramph ...	1988	3058982
[echographic and radiographic aspects of a case of burkitt's lymphoma].		2011	3060909
[etiologic structure of bacterial dysentery in the ussr in 1983-1985].	the etiological structure of dysentery in the ussr in 1983-1985 is characterized. sonne dysentery was found to prevail in the territories with adequate water supply, while dysentery caused by shigella flexneri prevailed at the territories with unsatisfactory water supply. s. dysenteriae and s. boydii were found to play a limited role in the etiology of dysentery. in the presence of global pandemic, an increase in the isolation rate of s. dysenteriae i in the ussr is observed. the data on the bio ...	1988	3061262
proceedings of the jerusalem meeting of the society for research into hydrocephalus and spina bifida, 1988.		2011	3062996
medicare: what it did for medicine it can do for dentistry.		2011	3063779
[the world-wide pandemic and drug resistance of the causative agent of grigor'ev-shiga dysentery].	one of the factors facilitating the global pandemic of grigor'ev-shiga dysentery is considered in detail. all shigella dysenteriae 1 strains, irrespective of the geographical zone of their spread, showed medicinal resistance. as pandemic developed, the spectrum of medicinal resistance constantly increased in all hyperendemic foci. the presence of pronounced relationships between the strains circulating in each of three hyperendemic foci and the strains circulating in different hyperendemic foci ...	1988	3064514
[use of prostacyclin in patients with ischemic stroke. a double-blind method ii].	thirty patients with acute ischaemic stroke were allocated randomly into a group treated with prostacyclin and a group receiving placebo. the treatment was started 24 to 72 hours after the onset of stroke. prostacyclin sodium (wellcome, u.k. or chinoin, hpr) or its solvent (glycine buffer) were administered intravenously once daily during 2 weeks in 6-hour infusions. prostacyclin was infused at rates of 2.5-5.0 ng/kg/min. statistically significant improvement appeared from the second day on in t ...	2010	3067105
[use of prostacyclin in patients with ischemic stroke. a double-blind method ii].	thirty patients with acute ischaemic stroke were allocated randomly into a group treated with prostacyclin and a group receiving placebo. the treatment was started 24 to 72 hours after the onset of stroke. prostacyclin sodium (wellcome, u.k. or chinoin, hpr) or its solvent (glycine buffer) were administered intravenously once daily during 2 weeks in 6-hour infusions. prostacyclin was infused at rates of 2.5-5.0 ng/kg/min. statistically significant improvement appeared from the second day on in t ...	2010	3067105
artificial feeding and hospitalization of infants in people's republic of china.		2011	3067138
differing expression of insulin-like growth factor i in the developing and in the adult rat cerebellum.	insulin-like growth factor i (igf-i; somatomedin c) is a trophic peptide of importance for the development of several tissues and organs. in the present study we have mapped the cellular distribution and dynamic changes of igf-i immunoreactivity in the rat cerebellum from its postnatal development to maturity. in vitro hybridization of igf-i mrna was used to demonstrate that the igf-i immunoreactive material was synthesized in the cerebellum during a limited time period of cerebellar differentia ...	1988	3067513
transplantation immunobiology in relation to neural grafting: lessons learned from immunologic privilege in the eye.	the principles of transplantation immunobiology are described and discussed in terms of their applicability to neural grafting, a newly emerging field dedicated to the ultimate goal of reconstituting central nervous system deficits with normally functioning tissue replacements. unique anatomic and physiologic features of the eye, which are responsible for the phenomenon of immunologic privilege, are compared with the brain and considered in terms of their relationship to the principles of transp ...	2011	3067548
alcohol-related symptoms in heterogeneous families of hospitalized alcoholics.	heterogeneity in the clinical symptoms of alcohol abuse was examined in 243 men and 305 women from families of hospitalized alcoholics, who had demonstrated different patterns of inheritance of susceptibility to alcoholism. discriminant analysis was utilized to identify nine alcoholic symptoms that distinguished male relatives of alcoholic men from those of alcoholic women. inability to abstain from alcohol, fighting and reckless driving while intoxicated, and alcohol treatment other than alcoho ...	2011	3067614
[academic dissertations of military pharmacists on the history of pharmacy].		2011	3067684
shiga toxin as inhibitor of protein synthesis.		1988	3068485
selective destruction of vagal sensory neurons using shigella cytotoxin.		1988	3068486
ciprostene in patients with peripheral vascular disease (pvd). an open-label, tolerance trial.	epoprostenol (prostacyclin) has been studied with various success in patients with peripheral vascular disease (pvd). we investigated the tolerance of a new, stable prostacyclin derivative ciprostene (9-beta-methyl carbacyclin) in 9 pvd patients. the drug was infused intravenously for 8 hours a day, once a week for 4 consecutive weeks, at a dose of 120 ng/kg/min. there were 6 men and 3 women with a mean age of 63 years (42-78). the pvd was verified by arteriography (9 patients) and by clinical f ...	2010	3068680
genetic relatedness of the basic replicon of the virulence plasmid in shigellae and enteroinvasive escherichia coli.	the regions required for replication (rep) and for stable maintenance (stb) of the 230 kilobase virulence plasmid, pmysh6000, in shigella flexneri 2a ysh6000 were identified on the restriction enzyme sa/l fragments, c and o, respectively. the stable plasmid, pmysh6610, consisting of adjacent fragments c and o was found to belong to the incompatibility group fl. in all strains of shigellae and enteroinvasive escherichia coli (eiec) tested, virulence plasmids were found to be highly homologous wit ...	1988	3070262
identity of molecular structure of shiga-like toxin i (vt1) from escherichia coli o157:h7 with that of shiga toxin.	the primary structures of the a and b subunits of shiga toxin and of shiga-like toxin i (vt1), isolated from the culture supernatants of shigella dysenteriae 1 and escherichia coli o157:h7, respectively, were analyzed by edman degradation of intact proteins and peptides in their digests with trypsin or achromobacter protease i and also by fast atom bombardment mass spectrometry of the digests. the results indicated that the a and b subunits of shiga toxin and shiga-like toxin i have the same pri ...	1988	3070268
a small nonconjugative plasmid encoded for streptomycin-resistant character in shigella dysenteriae.	conjugative transfers of drug-resistant plasmids in shigella dysenteriae type 1 isolated from the epidemic in west bengal were unsuccessful. the plasmids were non-transmissible in spite of having the fertility factor f, genetically labelled with kanamycin-resistant transposon tn903 (pws 7). a small 2.5 kb nonconjugative plasmid encoded for the streptomycin-resistant character. cured plasmid-less strains of s. dysenteriae 1 showed resistance to sulfonamides.	1988	3071516
ultrasound in the diagnosis of splenic abscess. case report.		2011	3071730
the rate of dam-mediated dna adenine methylation in escherichia coli.		1988	3074008
the t cell system in rheumatoid arthritis: activated or defective?	rheumatoid arthritis (ra) is characterised by the presence of activated lymphocytes in the synovial compartment, which are classically considered to be of particular importance to the pathogenesis of the disease. we have shown that activated lymphocytes are also found in the rheumatoid lymph nodes and peripheral blood, and that their proportions are increased in early or active disease. double-labelling experiments showed that t cell subsets within the activated circulating lymphocytes resemble ...	2010	3075073
plasmid profile as a useful marker of a shigella dysenteriae type 1 strain causing an epidemic of shigellosis.	plasmid profiles of 60 shigella dysenteriae type 1 isolates obtained during an epidemic period (april - october 1983) were compared with that of 74 other shigella species isolated during the same period of time and also with that of 20 s. dysenteriae type 1 isolates obtained before the epidemic period (1969 - 1982). all samples were collected during an epidemic of shigellosis from diarrhoeal patients being treated at the dhaka treatment centre of the international centre for diarrhoeal disease r ...	1988	3077946
[hearing disturbances and hearing loss of occupational origin in dentistry].		2011	3078965
triclonal gammopathy in a patient with plasma cell dyscrasia.	a triclonal gammopathy is an immunoglobulin abnormality in which three discrete monoclonal subpopulations of immunoglobulin molecules are present in a patient's serum. monoclonal and biclonal gammopathies have been studied extensively, but relatively little is known about the much rarer triclonal gammopathies. the case described below illustrates some of the difficulties that this condition can present to the clinician and the clinical laboratory.	2011	3079680
praziquantel in the acute porphyrias.		2011	3085239
levels and sources of stress in medical students.	levels of stress, as measured by the general health questionnaire, were assessed in 318 medical students in their fourth year at three british universities. mean scores were higher than those in other groups within the general population, and the estimated prevalence of emotional disturbance was 31.2%, a proportion similar to that reported in medical students in the united states. there were no differences in prevalence or in mean scores of stress between the sexes. twelve (4%) students reported ...	2009	3085772
hematologic disorders following prolonged use of intravenous fat emulsions in children.	abnormalities in hematologic parameters, associated with prolonged utilization of intravenous fat emulsion (ivfe), were observed in seven children on long-term cyclic total parenteral nutrition (tpn). all patients were receiving ivfe (intralipid 20%) 1 to 2 g/kg/24 hr as part of tpn, during 3 to 18 months. recurrent thrombocytopenia occurred in all seven patients. platelet lifespan, as measured with 111 indium was reduced. sea-blue histiocytes containing granulations and hemophagocytosis were se ...	2011	3086587
propofol and alfentanil infusion. a comparison with methohexitone and alfentanil for major surgery.	in 42 patients undergoing major surgery, anaesthesia was induced by intravenous alfentanil 10 micrograms/kg together with methohexitone 1.5 mg/kg or propofol 2 mg/kg. an infusion of six times these doses per hour was then started; the rate was varied subsequently as indicated by the monitoring of arterial blood pressure, heart rate, eeg and frontalis electromyogram. the mean duration of infusion was 76.7 minutes for propofol and 74.5 minutes for methohexitone and the infusion was stopped about 1 ...	2011	3089051
treating erythema chronicum migrans of lyme disease.	the efficacy of antibiotic treatment of 117 patients with erythema chronicum migrans of lyme disease was evaluated in terms of the necessity for retreatment and the prevention of the late manifestations of lyme disease. fifty-six patients with a minor form of the illness did not require retreatment and did not develop late manifestations following antibiotic treatment. three pregnant patients were included in this group. fourteen of sixty-one patients with a major form of the illness required re ...	2011	3093544
drg payments for raps slated in '87.		2011	3098671
characterization of purified shiga toxin from shigella dysenteriae 1.	shiga toxin was purified from the culture supernatant of shigella dysenteriae 1 by ammonium sulfate fractionation, deae-cellulose column chromatography and repeated chromatofocusing column chromatography. about 1.6 mg of purified shiga toxin was obtained from 15 liters of culture with a yield of about 27%. the molecular weight of purified shiga toxin was estimated to be 62,000. the toxin consisted of a and b subunits with molecular weights of about 30,000 and 5,000-6,000, respectively. the isoel ...	1986	3100915
aids policy development within the department of defense.		2011	3100984
[periodontal maintenance therapy].		2011	3101045
[transfer of r plasmids between shigella and e. coli strains in the intestine of patients with acute shigellosis].		1986	3103883
extracellular accumulation of proline, serine and trehalose in the haemolymph of osmoconforming brackish-water mosquitoes.	larvae of culex tarsalis, a mosquito, are capable of surviving and developing in dilutions of sea water ranging from 0 mosmol l-1 to 700 mosmol l-1. in waters more dilute than 400 mosmol l-1, the larvae osmoregulate, whereas in those more concentrated than 400 mosmol l-1, the osmotic strength of the haemolymph parallels that of the medium, i.e. the larvae osmoconform. over the full range of external concentrations tested, the larvae regulate the levels of na+, k+, mg2+, ca2+ and cl- in the haemo ...	2010	3108441
differences between saccharomyces cerevisiae and bacillus subtilis in secretion of human lysozyme.	saccharomyces cerevisiae secreted human lysozyme in the medium as an active form when the signal peptides of chicken lysozyme and a chicken lysozyme-aspergillus awamori glucoamylase hybrid were used, whereas it did not synthesize any human lysozyme protein by using the signal peptide of a. awamori glucoamylase. the secreted lysozyme was easily purified and crystallized. on the other hand, bacillus subtilis secreted an inactive human lysozyme, which seemed to have incorrect disulfide bonds, with ...	1987	3109419
differences between saccharomyces cerevisiae and bacillus subtilis in secretion of human lysozyme.	saccharomyces cerevisiae secreted human lysozyme in the medium as an active form when the signal peptides of chicken lysozyme and a chicken lysozyme-aspergillus awamori glucoamylase hybrid were used, whereas it did not synthesize any human lysozyme protein by using the signal peptide of a. awamori glucoamylase. the secreted lysozyme was easily purified and crystallized. on the other hand, bacillus subtilis secreted an inactive human lysozyme, which seemed to have incorrect disulfide bonds, with ...	1987	3109419
alternative pathway of complement activation by stimulated t lymphocytes. ii. elevation of cytotoxic potential against complement receptor-carrying cell lines.	exposure of lectin-stimulated (concanavalin a, phytohemagglutinin and pokeweed mitogen) blood lymphocytes to human serum or to purified c3 increased their cytotoxic capacity towards complement receptor positive targets such as raji and daudi cells. the lysis of complement receptor-negative lymphoblastoid cell lines was not influenced. the lytic capacity of lymphocytes exposed to 12-o-tetradecanoylphorbol 13-acetate was not elevated by human serum. lectin-stimulated lymphocytes were previously sh ...	1987	3111863
blood clonal b cell excess (cbe) at diagnosis in patients with non-hodgkin lymphoma (nhl). relation to clinical stage, histopathology and response to treatment.	untreated non-leukemic (lymphocytes less than or equal to 4.0 x 10(9)/l) patients with non-hodgkin lymphoma (nhl) of b cell type often show an excess of b cells in peripheral blood bearing the same light chain isotype as the lymph node tumor cells which may indicate a leukemic spread of the disease. the ratio between kappa- and lambda-bearing lymphocytes (normal range 1.0-3.3) was studied to evaluate the prognostic significance of clonal b cell excess (cbe) at diagnosis in 110 nhl patients. in t ...	2009	3115783
effects of cost sharing on physiological health, health practices, and worry.	in a randomized trial of the effects of medical insurance on spending and the health status of the nonaged, we previously reported that patients with limited cost sharing had approximately one-third less use of medical services, similar general self-assessed health, and worse blood pressure, functional far vision, and dental health than those with free care. of the 20 additional measures of physiological health studied here on 3,565 adults, people with cost sharing scored better on 12 measures a ...	2011	3119520
[local epidemic of neonatal listeriosis in upper austria--report of 20 cases].	in an 8 month period, 20 cases of listeriosis among neonates were seen in the federal district of upper austria. the majority of mothers reported influenza-like symptoms at the end of pregnancy. 19 cases were early-onset-infections, 6 infants developed meningitis. serotype 1/2a was isolated in 7 cases. all were of the same phage type. 15 neonates were successfully treated with aminopenicillin or penicillin. treatment with cephalosporin was ineffective. 4 mainly premature infants died, 1 was stil ...	1987	3119914