Publications

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simultaneous quantification of escherichia coli o157:h7 and shigella boydii using a visual-antibody-macroarray.being high throughput, rapid, automated, economical, convenient to operate and highly sensitive, protein arrays have been widely used in the analysis of tumor markers and veterinary drug residues. pathogenic microbes also can be detected qualitatively by dna array or protein array; however, their high throughput detection and quantification remains a big obstacle. to evaluate the potentiality of protein arrays for multiple quantitative detection of microorganisms with naked eye examination witho ...201526300105
in vitro probiotic characterization of lactobacillus strains from fermented radish and their anti-adherence activity against enteric pathogens.in this study, we evaluated the probiotic properties of lactobacillus plantarum, lactobacillus pentosus, and lactobacillus fermentum strains isolated from fermented radish. all the strains survived the simulated oro-gastrointestinal transit condition and showed significantly higher adherence to caco-2 cells compared with the probiotic strain lactobacillus rhamnosus gg. the strains showed broad-spectrum antimicrobial activity, autoaggregation, and coaggregation capacity with pathogens. furthermor ...201526382558
development of a cost-effective vaccine candidate with outer membrane vesicles of a tola-disrupted shigella boydii strain.our previous studies on outer membrane vesicles based vaccine development against shigellosis, revealed the inability of shigella to release significant amount of vesicles naturally, during growth. disruption of tola, one of the genes of the tol-pal system of gram negative bacterial membrane, has increased the vesicle release rate of a shigella boydii type 4 strain to approximately 60% higher. we also noticed the vesicles, released from tola-disrupted strain captured more ompa protein and lipopo ...201626878295
synthesis of newer 1,2,3-triazole linked chalcone and flavone hybrid compounds and evaluation of their antimicrobial and cytotoxic activities.the present study was carried out in an attempt to synthesize a new class of antimicrobial and antiplasmodial agents by copper catalyzed click chemistry to afford 25 compounds 10-14(a-e) of 1,4-disubstituted-1,2,3-triazole derivatives of chalcones and flavones. the structures of the newly synthesized compounds were established by elemental analysis, ir, (1)h nmr, (13)c nmr and mass spectral data. the newly synthesized compounds were evaluated for their antibacterial activity against gram positiv ...201626922227
antibacterial activity of a novel fatty acid (14e, 18e, 22e, 26e)-methyl nonacosa-14, 18, 22, 26 tetraenoate isolated from amaranthus spinosus.amaranthus spinosus linn. (amaranthaceae), commonly known as ''spiny pigweed'', is used in both indian traditional system and folk medicine for treatment of infectious diseases for a long time in several traditional herbal medicinal preparations. a novel fatty acid [(14e, 18e, 22e, 26e)-methyl nonacosa-14, 18, 22, 26 tetraenoate] is the major metabolite present.201627043360
genome diversity of shigella boydii.italic! shigella boydiiis one of the four italic! shigellaspecies that causes disease worldwide; however, there are few published studies that examine the genomic variation of this species. this study compares genomes of 72 total isolates; 28 italic! s. boydiifrom bangladesh and the gambia that were recently isolated as part of the global enteric multicenter study (gems), 14 historical italic! s. boydiigenomes in the public domain and 30 italic! escherichia coliand italic! shigellareference geno ...201627056949
structure and gene cluster of the o-antigen of enterobacter cloacae g3421.the o-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of enterobacter cloacae g3421 and studied by sugar analysis along with 1d and 2d (1)h and (13)c nmr spectroscopy. in addition, partial solvolysis with anhydrous trifluoroacetic acid was applied, which cleaved selectively the α-l-rhamnopyranosidic linkages. the following structure of the branched hexasaccharide repeating unit was established. the o-polysaccharide studied shares the β-l-rhap-(1→4)-α-l-rhap-(1→2)-α ...201627131290
shigella diversity and changing landscape: insights for the twenty-first century.shigella is a pathovar of escherichia coli comprising four groups, shigella flexneri, shigella sonnei, shigella dysenteriae, and shigella boydii, each of them, with the exception of s.sonnei, comprising several serotypes. shigella accounts for the majority of dysentery causing infections occurring world-wide each year. recent advancements in the shigella field have led to a better understanding of the molecular mechanisms underlying host epithelial cell invasion and immune cell function manipula ...201627148494
bacterial assessment of food handlers in sari city, mazandaran province, north of iran.food handlers with poor personal hygiene could be potential sources of infection due to pathogenic bacteria. this study was designed to determine the prevalence of bacterial infestation among the food handlers attending the public health center laboratory in sari, northern iran for annual check-up. this study was performed from september 2013 to august 2014. stool samples, fingernail specimens of both hands and nasal swabs were collected from 220 male and female food handlers of different jobs, ...201727435639
molecular characterisation of quinolone-resistant shigella strains isolated in tehran, iran.over the past few years, the number of shigella strains resistant to nalidixic acid has increased and has made the selection of effective antimicrobial therapy more difficult. the purpose of this study was to investigate the molecular mechanism of quinolone resistance in shigella strains. shigella strains isolated from 1100 diarrhoeal patients in tehran, iran, were assessed for their susceptibility to nalidixic acid prior to pcr-rflp and sequence analysis of their quinolone resistance genes. amo ...201627436462
a review on sero diversity and antimicrobial resistance patterns of shigella species in africa, asia and south america, 2001-2014.shigella, gram negative bacterium, is responsible for shigellosis/bacillary dysentery. it is a global concern although it predominates in developing countries. these are shigella dysenteriae, shigella flexneri, shigella boydii and shigella sonnei. drug resistance by shigella species is another headache of the world. therefore; this study aimed to review distribution of shigella serogroups and their antimicrobial patterns carried out in africa, asia and south america.201627576729
structure and genetics of the o-antigens of escherichia coli o182-o187.o-polysaccharides (opss) were obtained by mild acid degradation of the lipopolysaccharides of escherichia coli o182-o187, and their structures were established by sugar analysis, smith degradation, and (1)h and (13)c nmr spectroscopy. in addition to the monosaccharides that occur often in e. coli opss (d-glc, d-gal, d-man, d-glcnac, d-galnac, d-glca, l-fuc, d-rib), a number of less common components were identified as the ops constituents, including 2-acetamido-2-deoxy-l-quinovose and 4-deoxy-4- ...201627710814
the first report of a fully sequenced resistance plasmid from shigella boydii.the purpose of this study was to characterize mechanisms of plasmid-mediated antimicrobial resistance in shigella boydii. s. boydii strain 2246 with resistance to ciprofloxacin, ceftriaxone and azithromycin was isolated from a human case of watery diarrhea in a chinese public hospital. resistance in strain 2246 to ceftriaxone and azithromycin was attributable to the presence of blactx-m-14, and erm(b) and mph(a), respectively, which were co-located on a multidrug-resistant (mdr) plasmid p2246-ct ...201627766094
shigellosis in nepal: 13 years review of nationwide surveillance.shigella is a major cause of gastroenteritis especially in children. in developing countries, the incidence is frequent and results are often life threatening. changing epidemiology and emerging antibiotic resistance warrants continuous monitoring of susceptibility. the present study highlights the changing epidemiology and drug resistance patterns of shigella isolated at different hospitals of nepal over a period of 13 years (jan. 2003-dec. 2015).201627814742
antimicrobial assay and genetic screening of selected freshwater cyanobacteria and identification of a biomolecule dihydro-2h-pyran-2-one derivative.explorations of freshwater cyanobacteria as antimicrobial (bacteria, fungi and methicillin-resistant staphylococcus aureus (mrsa) strains) drug resource using bioassay, nrps (non-ribosomal polypeptide synthetase) and pks (polyketide synthase) genes, as well as in silico approach.201728004519
rational design of reversible inhibitors for trehalose 6-phosphate phosphatases.in some organisms, environmental stress triggers trehalose biosynthesis that is catalyzed collectively by trehalose 6-phosphate synthase, and trehalose 6-phosphate phosphatase (t6pp). t6pp catalyzes the hydrolysis of trehalose 6-phosphate (t6p) to trehalose and inorganic phosphate and is a promising target for the development of antibacterial, antifungal and antihelminthic therapeutics. herein, we report the design, synthesis and evaluation of a library of aryl d-glucopyranoside 6-sulfates to se ...201728192710
virulotyping of shigella spp. isolated from pediatric patients in tehran, iran.shigellosis is a considerable infectious disease with high morbidity and mortality among children worldwide. in this survey the prevalence of four important virulence genes including ial, ipah, set1a, and set1b were investigated among shigella strains and the related gene profiles identified in the present investigation, stool specimens were collected from children who were referred to two hospitals in tehran, iran. the samples were collected during 3 years (2008-2010) from children who were sus ...201728218003
in vitro anti-oxidant and anti-microbial potentiality investigation of different fractions of caryota urens leaves.caryota urens is a member of the arecaceae family and a common plant in the southeast asian region. this plant has been reported as an anti-microbial agent in recent years. thus, we aimed to find out the mic (minimum inhibitory concentration) against different pathogenic microorganism.201628536384
the enterohemorrhagic escherichia coli effector espw triggers actin remodeling in a rac1-dependent manner.enterohemorrhagic escherichia coli (ehec) is a diarrheagenic pathogen that colonizes the gut mucosa and induces attaching-and-effacing lesions. ehec employs a type iii secretion system (t3ss) to translocate 50 effector proteins that hijack and manipulate host cell signaling pathways, which allow bacterial colonization and subversion of immune responses and disease progression. the aim of this study was to characterize the t3ss effector espw. we found espw in the sequenced o157:h7 and non-o157 eh ...201728630074
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