| synthetic peptides of shiga toxin b subunit induce antibodies which neutralize its biological activity. | shiga toxin b chain, the binding subunit of shiga toxin, was recently purified; and the amino acid sequence of this 7,716-dalton polypeptide was determined (n.g. seidah, a. donohue-rolfe, c. lazure, f. auclair, g. t. keusch, and m. chretien, j. biol. chem. 261:13928-13931, 1986). in the present study, synthetic peptides corresponding to three overlapping sequences from the n-terminal region of this subunit were prepared. the peptides synthesized consisted of residues 5 to 18, 13 to 26, and 7 to ... | 1988 | 3286503 |
| conservation of an atp-binding domain among reca proteins from proteus vulgaris, erwinia carotovora, shigella flexneri, and escherichia coli k-12 and b/r. | the purified reca proteins encoded by the cloned genes from proteus vulgaris, erwinia carotovora, shigella flexneri, and escherichia coli b/r were compared with the reca protein from e. coli k-12. each of the proteins hydrolyzed atp in the presence of single-stranded dna, and each was covalently modified with the photoaffinity atp analog 8-azidoadenosine 5'-triphosphate (8n3atp). two-dimensional tryptic maps of the four heterologous reca proteins demonstrated considerable structural conservation ... | 1988 | 3286605 |
| escherichia coli o157:h7, an emerging gastrointestinal pathogen. results of a one-year, prospective, population-based study. | to examine the incidence of escherichia coli o157:h7 enteric infections in the united states and to evaluate the vehicles of transmission for sporadic cases, we conducted a one-year, population-based study at a large health maintenance organization (hmo) in the puget sound area of washington state. all stool specimens submitted for culture to the hmo laboratory were screened for e coli o157:h7; the organism was identified in 25 (0.4%) of 6485 stool specimens. all patients with e coli o157:h7 ide ... | 1988 | 3286919 |
| large plasmid in shigella boydii is responsible for epithelial cell penetration. | a large plasmid in a virulent shigella boydii 5 strain was transferred to plasmid-cured avirulent strains of s. boydii 5, s. boydii 12, s. sonnei form ii, and escherichia coli k12. the transconjugants acquired the ability to invade tissue culture cells, which indicated that the large plasmid in s. boydii is responsible for epithelial cell invasiveness. | 1988 | 3287107 |
| [clinico-immunological characteristics of mixed intestinal infections in children]. | | 1988 | 3287323 |
| detection of specific bacterial enzymes by high contrast metal chelate formation. part ii. specific detection of escherichia coli on multipoint-inoculated plates using 8-hydroxyquinoline-beta-d-glucuronide. | the use of 8-hydroxyquinoline-beta-d-glucuronide for the demonstration of beta-glucuronidase activity within the family enterobacteriaceae has been investigated. the compound has been shown to be an effective substrate for the bacterial enzyme and the michaelis constant for the association has been determined. a multipoint-inoculated method was employed to test 400 routine clinical isolates including those of urinary and faecal origin. when grown on an agar-based medium containing the substrate ... | 1988 | 3287802 |
| survival of enteric pathogens in common beverages: an in vitro study. | this in vitro study was undertaken to determine the potential for survival of enteric pathogens in common drinking beverages. three carbonated soft drinks, two alcoholic beverages, skim milk, and water were inoculated with salmonella, shigella, and enterotoxigenic escherichia coli, and quantitative counts were performed over 2 days. our studies showed poorest survival of all three organisms in wine, and greatest growth in milk and water. beer and cola allowed survival of small numbers of salmone ... | 1988 | 3287903 |
| [changes in the degree of sensitivity to antibiotics of shigella isolated from patients with dysentery in a city infectious disease hospital over the past 8 years]. | changes in sensitivity of shigella and in particular shigella flexneri isolated from dysentery patients in a city infection hospital within 8 years i.e. from 1977 to 1985 were followed. it was shown that the changes were to the greater extent connected with preferable use of certain antibiotics in the hospital. | 1988 | 3288150 |
| [an investigation on secondary attack rate in families and environment contamination of bacillary dysentery in urban and rural areas]. | | 1988 | 3289748 |
| [a preliminary observation on the bactericidal effect of chlorinated trisodium phosphate]. | | 1988 | 3289749 |
| [epidemic shiga bacillus dysentery in china]. | | 1988 | 3289750 |
| suggestion to supplement shigella flexneri classification scheme with the subserovar shigella flexneri 4c: phenotypic characteristics of strains. | a new serovar of shigella flexneri has been isolated with increasing frequency in the ussr. it has the antigenic formula iv:7,8. we suggest that it be incorporated into the classification scheme as s. flexneri subserovar 4c. the 212 strains of this proposed subserovar examined to date display biochemical and serological properties typical of the species, are sensitive to the genus-specific bacteriophage, and cause keratoconjunctivitis in the sereny test. | 1988 | 3290245 |
| analysis and genetic manipulation of shigella virulence determinants for vaccine development. | shigellosis is a major public health problem in developing countries. current epidemics of shigella dysenteriae serotype 1 strains are particularly serious and are characterized by high mortality rates. a high proportion of the isolates are resistant to many of the antibiotics currently in use in these countries, a feature which seriously compromises clinical treatment of the infections. efficacious vaccines are thus urgently needed. basic studies on shigella virulence factors, infections in lab ... | 1988 | 3291449 |
| biotyping of shigella sonnei (a note). | one thousand and fifty-nine shigella sonnei strains were submitted to biotyping and colicine typing. simultaneous use of biotyping and colicine typing may be considered of value in epidemiological tracing. | 1988 | 3293342 |
| molecular cloning of invasion plasmid antigen (ipa) genes from shigella flexneri: analysis of ipa gene products and genetic mapping. | tn5-tagged invasion plasmid dna (pwr110) from shigella flexneri serotype 5 (strain m90t) was cloned into the expression vector lambda gt11. recombinant phage (lambda gt11sfl) expressing pwr110-encoded polypeptide antigens were identified by using rabbit antisera directed against s. flexneri m90t invasion plasmid antigens. antigens encoded by lambda gt11sfl recombinant phage were characterized by reacting affinity-purified antibodies, eluted from nitrocellulose-bound plaques of lambda gt11sfl rec ... | 1987 | 3294797 |
| bacterial invasion of fibroblasts induces interferon production. | although bacteria and/or bacterial products have been shown to induce interferon (ifn) production in cells of the immune system, the ability of non-lymphoid cells to produce ifn in response to bacterial products and/or bacterial invasion is currently unknown. in this study we examined murine and human fibroblasts for their ability to produce ifn in response to challenge with the invasive bacteria shigella flexneri and enteroinvasive escherichia coli. both human and murine primary cell cultures p ... | 1987 | 3295042 |
| risk factors for development of hemolytic uremic syndrome during shigellosis. | | 1987 | 3295160 |
| toxic megacolon complicating shigellosis. | | 1987 | 3295219 |
| [evaluation of the effectiveness of selective-differentiating culture media and their sets used to isolate shigella and salmonella]. | | 1987 | 3295427 |
| [analytical study of an epidemic of bacillary dysentery in rwanda. epidemiologic and bacteriologic aspects]. | from january 1985 to march 1985, an epidemic of bacillary dysentery stroke one of the region in north rwanda. the area struck, only 160 km2, is densely populated (720 inhabitants/km2). multiresistant shigella dysenteriae n. 1 is the only responsible factor. the study of this epidemic shows that, from an endemic disease, burst out can occur in a narrow area and in a short interval of time (7 weeks). the main risk factors have been identified on individual as well as collective level. the authors ... | 1987 | 3295462 |
| the relative activity of endotoxins of different origins in the lal gelation method and in the lal chromogenic substrate method. | | 1987 | 3295884 |
| [dynamics of bacterial polysensitization in acute dysentery in children depending on the species of the causative agent]. | studies on polysensitization, evaluated by the neutrophil damage index, have been made with the use of dysenterin and the allergens of hemolytic staphylococci, enterococci and escherichia coli. the studies have revealed that both specific and nonspecific sensitization to hemolytic staphylococci and enterococci is higher in dysentery caused by shigella flexneri than in dysentery caused by s. sonnei and has the tendency to increase in the course of the disease. | 1987 | 3296580 |
| shiga-like toxin production and attaching effacing activity of escherichia coli associated with calf diarrhea. | four hundred twenty-nine isolates of escherichia coli from calves were tested for the production of hela cell cytotoxin(s). isolates that produced enough cytotoxin to be detected in culture supernatants of iron-depleted broth were considered to produce increased amounts of cytotoxins. isolates also were tested for homology with a dna probe for a gene that encodes localized adherence of human enteropathogenic e coli. four isolates produced increased amounts of cytotoxin that was neutralized by sh ... | 1987 | 3296889 |
| humoral response in mice immunized with outer membrane proteins of shigella flexneri. | intraperitoneal immunization of mice with outer membrane proteins (omp) of sh. flexneri induced in the animals a synthesis of specific antibodies. their level determined by elisa test was found to be relatively low in the sera of animals immunized with a single dose (10 micrograms) of omp; it was markedly higher in mice immunized with two doses of omp, and very high after three fold immunization. the specific antibodies maintained in the animals for 8-16 weeks after immunization. anti-omp sera g ... | 1986 | 3296993 |
| characterization of outer membrane proteins of virulent phase i shigella sonnei strains and their avirulent phase i derivatives. | comparison of polyacrylamide gel electrophoretic protein profiles of four isogenic sets of virulent phase i sh. sonnei strains and their avirulent phase i cells revealed no differences in outer membrane protein composition between virulent and avirulent derivatives. however, significant qualitative and quantitative differences were found in composition of major outer membrane proteins between strains of different origin. all four strains tested contained one major protein of 33k. this protein wa ... | 1986 | 3296995 |
| [determination of shigella sonnei in milk using the coagglutination reaction]. | | 1987 | 3297931 |
| shiga and shiga-like toxins. | | 1987 | 3299029 |
| nucleotide sequence of the shiga-like toxin genes of escherichia coli. | we have determined the nucleotide sequence of the slta and sltb genes that encode the shiga-like toxin (slt) produced by escherichia coli phage h19b. the amino acid composition of the a and b subunits of slt is very similar to that previously established for shiga toxin from shigella dysenteriae 1, and the deduced amino acid sequence of the b subunit of slt is identical with that reported for the b subunit of shiga toxin. the genes for the a and b subunits of slt apparently constitute an operon, ... | 1987 | 3299365 |
| [growth and development of a population of the causative agent during an infection]. | | 1987 | 3299739 |
| multiple enteric pathogens in a patient with diarrhoea. | a woman who recently arrived in canada from india developed a febrile, diarrhoeal illness. stool culture revealed the presence of salmonella typhi, campylobacter fetus subspecies jejuni and shigella flexneri. concurrent infection with these three pathogens does not appear to have been previously reported. the possibility of multiple pathogens should be considered in travellers who fail to respond to treatment of a single organism. | 1987 | 3299934 |
| corneal ulcer caused by shigella flexneri in an infant. | | 1987 | 3300355 |
| in vitro activity and beta-lactamase stability of a new monobactam, b0-1165. | b0-1165 is a 1-carboxy-1-cyclopropoxyamino,4-fluoromethyl monobactam. it inhibited the majority of escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, citrobacter diversus, aeromonas hydrophila, proteus mirabilis, proteus vulgaris, providencia rettgeri, providencia stuartii, yersinia enterocolitica, haemophilus influenzae, neisseria gonorrhoeae, and salmonella and shigella species at less than or equal to 0.125 microgram/ml. overall, its in vitro activity was similar to that of aztreona ... | 1987 | 3300528 |
| live dysentery vaccine "vadizen" (t32-istrati) immunogenicity against shigella-like challenge in animals. | | 1986 | 3300598 |
| ultrastructural study on the bacterial symbiont in giardia lamblia. | | 1986 | 3300600 |
| shigella sonnei myocarditis. | a 19-year-old male was admitted to our department because of high fever, abdominal pain, and diarrhea. electrocardiogram (ecg) on admission revealed diffuse st-t changes which persisted for 5 days. stool culture grew shigella sonnei. we conclude that the infection with shigella sonnei was the cause for these findings which were most probably due to myocarditis. to the best of our knowledge myocarditis as a complication of shigella infestation has never been reported. | 1987 | 3301121 |
| [reye-like syndrome due to shigella flexneri in a child]. | | 1987 | 3301584 |
| antimicrobial therapy of non-typhi salmonella and shigella infection. | | 1987 | 3301781 |
| treatment of bacillary dysentery: a comparison between enoxacin and nalidixic acid. | a prospective randomized study has been conducted in rwanda to compare the efficacy of enoxacin versus nalidixic acid for the treatment of bacillary dysentery in adults. seventeen patients received nalidixic acid and 21 received enoxacin. all isolates (37 shigella, 1 campylobacter) were sensitive in vitro to the two drugs. all patients showed a favourable clinical and bacteriological response and the results obtained with both drugs were comparable. enoxacin seems to be a good alternative to nal ... | 1987 | 3301783 |
| clinical presentation of shigellosis during the 1984 epidemic of bacillary dysentery in west bengal. | | 1987 | 3301795 |
| shigellosis in bangladesh: a reply. | | 1987 | 3302055 |
| physicochemical characterization of a and b subunits of shiga toxin and reconstitution of holotoxin from isolated subunits. | the a and b subunits of shiga toxin were isolated by high performance liquid chromatography and their physicochemical properties were examined. the a subunit of shiga toxin purified from culture supernatant was not nicked, but it could be nicked in vitro by trypsin. the isoelectric points of the a and b subunits were determined to be 8.2 and 5.8, respectively. amino acid compositions of the two subunits were also determined. the isolated a and b subunits were reconstituted to form active holotox ... | 1987 | 3302622 |
| [study of the sensitivity of enteropathogenic bacteria to nifurzide]. | nifurzide, a synthetic antimicrobial agent of the nitrofuran group, was tested on 320 enteropathogenic bacteria. minimal inhibition concentrations (mic) (expressed in mg/l) were determined using the standard agar dilution technique. the mics were excellent for campylobacter spp. (97 strains), mic 50:0.5, mic 90:0.5; slightly higher for shigella spp. (50 strains) and aeromonas spp. (22 strains), mic 50:2, mic 90:4; and highest for salmonella spp. (100 strains) and yersinia enterocolitica (49 stra ... | 1987 | 3302859 |
| water-melon as a vehicle of transmission of shigellosis. | an epidemic of shigella sonnei infection is described in which the only common source of infection was the ingestion of water-melon. bacteriological studies demonstrated that s. sonnei bacteria injected into water-melons could multiply to infective doses. therefore water-melons should not be excluded as a possible source of s. sonnei infection. | 1987 | 3303300 |
| [reaction of the hematopoietic system in mice to the administration of virulent and avirulent strains of shigella flexneri 2a]. | the influence of live bacteria and antigenic preparations of virulent and avirulent stains of shigella flexneri 2a on the endogenous splenic colony formation in murine hematopoietic cells has been studied. the different stimulating activity of live microbial cells of virulent and avirulent strains shigella flexneri 2a and their antigenic preparations on the endogenous colony formation has been shown. the effect observed depends on the preparation doses and time of their administration before irr ... | 1987 | 3304434 |
| [a reservoir of shigella of avian origin: herons and birds of prey of the zoological garden in tananarive]. | the authors record the bacteriological characters of four shigella strains: s. dysenteriae 2 and s. boydii 9, respectively isolated in a bird-of-prey and in three herons, kept in the zoological garden in antananarivo. the significance of this malgasian avian reservoir of shigella is discussed. | 1987 | 3304678 |
| [cloning and primary structure of shigella toxin genes]. | | 1987 | 3304914 |
| plasmid-encoded expression of lipopolysaccharide o-antigenic polysaccharide in enteropathogenic escherichia coli. | the role of a plasmid in the virulence activity of an enteropathogenic escherichia coli (epec) strain belonging to serotype 0111:nm was examined. epec strain b171, which is resistant to chloramphenicol, streptomycin, sulfathiazole, and tetracycline, harbors a 54-megadalton plasmid, pyr111, and exhibits localized adherence (la) with hela cells. curing the plasmid yielded strain b171-4, which had lost the ability to exhibit la, resistance to the antibiotics, and the lipopolysaccharide (lps) o-anti ... | 1987 | 3305360 |
| the penicillins. | the penicillin family of antibiotics is ever expanding and remains an important part of our antimicrobial armamentarium. these medications generally have bactericidal activity, excellent distribution throughout the body, low toxicity, and efficacy against infections due to susceptible organisms. the clinical introduction of aqueous penicillin g for treatment of streptococcal and staphylococcal infections was an important pharmacologic landmark. the emergence of penicillinase-producing staphyloco ... | 1987 | 3306181 |
| adherence to and penetration of cultured cells by an invasive strain of escherichia coli: an ultrastructural study. | the penetration process of an enteroinvasive strain of escherichia coli 0124 into hep-2 cells has been studied by electron microscopy. bacteria penetrated into cultured cells by a phagocytosis-like mechanism, and multiplied within the vacuoles as well as in the cytoplasmic matrix. on the whole, the infection cycle of eiec appeared to be similar to that described for shigella. the stain examined has also been found to elaborate a 40 nm-thick glyocalix, which is probably involved in the recognitio ... | 1987 | 3306293 |
| a new chromogenic test for the detection of urokinase in the genus shigella. | a simple test for detection of urokinase in shigella is described. the test is performed by suspending a loopful of bacteria in 100 microliters of a buffered 1mm solution of benzoyl-beta-alanyl glycyl-arginyl-4-nitroanilide acetate (chromozym u). enzymatic activity is revealed by formation of a yellow colour after 24 h of incubation at 37 degrees c. the test is able to differentiate serotypes of shigella dysenteriae, shigella flexneri, shigella boydii. the results suggest the possibility of incl ... | 1987 | 3306294 |
| elisa detectable virulence marker antigen of enteroinvasive escherichia coli is coded by a 140 megadalton plasmid. | the elisa reactive virulence marker antigen, characteristic of enteroinvasive escherichia coli and virulent shigella, expressed in an e. coli k-12 recipient after the 140 md plasmid of an enteroinvasive e. coli strain had been introduced into it. similarly to the epithelial cell penetration and congo red binding capacities--known to be coded by the above plasmid--the virulence marker antigen expressed also only at 37 degrees c but not at 30 degrees c. these data suggest the plasmid coded nature ... | 1986 | 3307275 |
| [molecular-genetic structure and incompatibility of the nonconjugative enterobacterial r plasmids pkmr281 and pkmr285]. | physical maps of enterobacteriaceae nonconjugative plasmids pkmr281 (sm, su, molecular weight of 6 kb) and pkmr285 (sm, su tc, molecular weight of 9 kb) were constructed for endonucleases ecori, psti, ecorv, smai, bglii, sali and pvuii. the genes controlling production of aminoglycoside-3''-phosphotransferase and dihydropteroate synthetase of type ii and the genetic tetracycline resistance determinant of class a were localized on the plasmids. heteroduplex analysis of plasmids pkmr281 and pkmr28 ... | 1987 | 3307606 |
| [current antimicrobial resistance of salmonella typhi, salmonella enteritidis and shigella sp]. | | 1987 | 3307812 |
| iron regulation of shiga-like toxin expression in escherichia coli is mediated by the fur locus. | shiga-like toxin is an iron-regulated cytotoxin quite similar to shiga toxin from shigella dysenteriae 1. the structural genes for shiga-like toxin in escherichia coli (slta and sltb) appear to be transcribed as an operon from a promoter upstream of slta. we used a gene fusion between the promoter and proximal portion of slta with the gene for bacterial alkaline phosphatase to assess the regulation of toxin expression. growth in low-iron conditions resulted in a 13- to 16-fold increase in alkali ... | 1987 | 3308853 |
| differentiation of shigella by esterase electrophoretic polymorphism. | the electrophoretic mobilities of four esterases (a, b, c, and i) of 182 strains of shigella dysenteriae, s. flexneri, s. boydii and s. sonnei were compared to those of 636 strains of escherichia coli from various origins, including the alkalescens dispar group and enteroinvasive strains. discriminant analysis of the distribution of esterases among the strains revealed that shigella could be distinguished from e. coli by differences in the distribution of allozymes of esterases c and i. principa ... | 1987 | 3309172 |
| atrio-ventricular block associated with shigella flexneri infection. | | 1987 | 3311739 |
| entry of shigella flexneri into hela cells: evidence for directed phagocytosis involving actin polymerization and myosin accumulation. | the enteroinvasive bacterium shigella flexneri expresses a plasmid-mediated capacity to penetrate into nonphagocytic cells. by using 7-nitrobenz-2-oxa-1,3-diazole-phallacidin (nbd-phallacidin), a fluorescent dye which specifically stains microfilaments, we observed condensations of filamentous actin underneath the plasma membrane of hela cells which interacted with the invasive isolate m90t. as demonstrated by indirect immunofluorescence with the antimyosin monoclonal antibody cc-212, myosin acc ... | 1987 | 3312007 |
| introduction of shigella flexneri 2a type and group antigen genes into oral typhoid vaccine strain salmonella typhi ty21a. | for protection against dysentery caused by shigella flexneri 2a, an in vivo-constructed recombinant plasmid with genes specifying the s. flexneri type and group antigens located near the pro (min 6) and his (min 44) chromosomal markers, respectively, was made and transferred to the gale salmonella typhi strain ty21a. strain ty21a carrying this recombinant plasmid was shown by immunological and biochemical analyses to express the s. flexneri 2a type and group antigens. mice immunized with this va ... | 1987 | 3312013 |
| expression of campylobacter jejuni invasiveness in cell cultures coinfected with other bacteria. | enteroinvasive salmonella, shigella, and escherichia coli strains were found to exert an effect which rendered campylobacter jejuni capable of intracellular localization in epithelial cells in vitro. when monolayers of hep-2 or a-549 cells were challenged with pure cultures of c. jejuni or campylobacter coli, none of the eight strains tested invaded the cells. in contrast, four of these strains were able to localize intracellularly when the cells were challenged with a mixture of campylobacters ... | 1987 | 3312015 |
| interferon-induced protein synthesis inhibits endocytosis of bacteria in epithelial cells. | previous studies have shown that human leukocyte interferon (ifn), ifn-gamma, and recombinant (r) ifn-alpha-a protect cultured human cells against the invasiveness of salmonellae, shigellae, and enteroinvasive escherichia coli. to differentiate between effect on endocytosis and adhesiveness, hep-2 cells were pretreated with cytochalasin b to block endocytosis, and thereafter treated with ifn. although a significant reduction of adhesion of bacteria to hep-2 cells was observed, this reduction was ... | 1987 | 3312425 |
| trimethoprim-resistant shigella and enterotoxigenic escherichia coli strains in children in thailand. | the percentage of shigella and enterotoxigenic escherichia coli (etec) strains resistant to trimethoprim (tmp)-sulfamethoxazole isolated from children with diarrhea at the outpatient department of the children's hospital in bangkok increased from 3 and 0%, respectively, in 1982 to 29% and 25% in 1986. one hundred thirty-nine shigella and 22 etec strains resistant to greater than 1024 micrograms/ml of trimethoprim (tmpr) isolated from children with diarrhea in bangkok in 1984 and 1985 were analyz ... | 1987 | 3313233 |
| [bacillary dysentery 1985]. | | 1987 | 3313536 |
| [a case from practice (93). patient: mrs. c. l., born 1933, a swiss female living in tunisia]. | | 1987 | 3313624 |
| in vitro activity of ciprofloxacin compared to trimethoprim-sulfamethoxazole against campylobacter spp., shigella spp. and enterotoxigenic escherichia coli causing travellers' diarrhea in egypt. | the in vitro activity of ciprofloxacin against bacterial enteropathogens isolated from cases of travellers' diarrhea in egypt was compared to trimethoprim (tmp) and trimethoprim-sulfamethoxazole (sxt). no resistance to ciprofloxacin was noted for any of the campylobacter jejuni/coli, shigella spp., and enterotoxigenic escherichia coli strains examined. however, resistance to tmp and sxt was noted among these same strains. because of its broad spectrum and lack of resistance, ciprofloxacin is pot ... | 1987 | 3313681 |
| hemolytic uremic syndrome associated with shigellosis: report of two cases. | two patients with hemolytic uremic syndrome admitted to udornthani hospital, thailand, were reported. the preceding illness was shigellosis. the stool culture was positive for shigella dysenteriae in one patient and shigella flexneri in another. the management was successful with peritoneal dialysis in one and symptomatic treatment in another. both patients had complete recovery. | 1987 | 3313740 |
| [prospects for using thymalin in treating bacterial intestinal infections]. | | 1987 | 3314133 |
| technological investigations with attenuated strains of shigella for production of live vaccines. 1. cultivation in a fermenter of an attenuated strain s. flexneri 2a with two markers. | a method was developed for cultivation of the strain shigella flexneri 2a 77 with two attenuated markers. it ensures the preservation of the initial properties of the strain. the regimen for control of dissolved oxygen level was optimized. it was established that yeast extract is a necessary component of the nutritive medium used. the role of the phase and quantity of the inoculum for growth of the bacterial population has been clarified. fresh fermenter culture, applied in rats, shows a high im ... | 1987 | 3314220 |
| transmissible drug resistance among shigellae and other enterobacteriaceae isolated from diarrhoeic human beings in ibadan, nigeria. | antibiotic resistance patterns and the distribution of r-factors among shigella, salmonella and escherichia coli isolated from diarrhoeic human beings were studied. nine multiple antibiotic resistance patterns among which pns tck was the most common were observed. all the shigella, salmonella and eighteen escherichia coli isolates transferred part of their r determinants respectively to e. coli k12. it is thought that enforcement of the law regulating the sales and judicious use of antibiotic in ... | 1987 | 3314255 |
| [the mouse paw edema test for titrating shigella toxin and antitoxin and for determining the safety of the anatoxin]. | the method for the titration of shigella dysenteriae i neurotoxin, toxoid prepared from this toxin and different antitoxic preparations in the mouse paw edema test has been developed. the quantitative determination of antitoxin is based on the neutralization test. the conditions of titration (the dilutions of the neurotoxin and the neutralization doses of the antitoxin), as well as criteria for the evaluation of the positive or negative results, have been established. | 1987 | 3314277 |
| bactericidal activities of selected organic n-halamines. | the bactericidal efficacies of three organic n,n'-dihalamine disinfectants in the class of compounds termed imidazolidinones were determined for combinations of ph, temperature, and water quality treatments by using staphylococcus aureus and shigella boydii as test organisms. the compound 1,3-dibromo-4,4,5,5-tetramethyl-2-imidazolidinone was found to be the most rapidly acting bactericide, especially under halogen-demand-free conditions. the mixed n,n'-dihalamine 1-bromo-3-chloro-4,4,5,5-tetrame ... | 1987 | 3314705 |
| shigella bacteremia in adults. a report of five cases and review of the literature. | shigella bacteremia is rare, occurring mainly in children. we describe five adult patients with shigella bacteremia and review data on 22 cases reported in the english-language medical literature. eighteen (67%) of 27 patients had either an underlying condition or were aged older than 65 years. most patients had clinical signs of acute febrile gastroenteritis. however, in six patients, the organism was not isolated from stool. species isolated from blood included shigella flexneri in 11 patients ... | 1987 | 3314766 |
| a 2d-1h-n.m.r. study of some shigella flexneri o-polysaccharides. | a 1h-n.m.r. study of the o-polysaccharides from different types of shigella flexneri has been performed. with the aid of 2d-n.m.r. techniques, namely, j-resolved, spin-spin correlation, and noesy experiments, most of the structural features of these polysaccharides could be deduced. sequences could generally be obtained from the noesy experiments. when using a prolonged mixing time in these experiments, cross-peaks due to spin diffusion from one anomeric proton to the anomeric proton of an adjac ... | 1987 | 3315202 |
| shigellemia and hemolytic-uremic syndrome: a case report. | | 1987 | 3316466 |
| appendicitis associated with shigella sonnei dysentery. | | 1987 | 3316626 |
| [biological properties of opportunistic microorganisms isolated from patients with acute intestinal diseases]. | the comparative study of the incidence of the pathogenicity markers (dnaase, rnaase, phosphatase and hemolytic activity) in shigellae and salmonellae, acknowledged as the causative agents of intestinal infections, and in opportunistic bacteria isolated from the feces of patients with acute intestinal diseases and healthy persons has been made. the study has revealed that dnaase and rnaase activity occurs most frequently in shigella flexneri, in salmonellae and in opportunistic enterobacteria iso ... | 1987 | 3318225 |
| shigellosis at a caribbean resort. hamburger and north american origin as risk factors. | during a three-week period in 1984, 339 tourists and expatriate employees with diarrhea visited the infirmary at a caribbean resort club. epidemiologic studies suggested that over 60% of the 1,893 tourists at the resort club during that time may have been ill. shigella flexneri 4a was isolated from seven of 18 stool specimens. a few cases of diarrheal illness occurred at the resort club before the onset of the outbreak, which was temporally related to illness in a butcher. north american residen ... | 1987 | 3318410 |
| elimination of a virulence plasmid from shigella sonnei and escherichia coli by antibiotics. | plasmid pwr105 is a non-self-conjugative plasmid conferring enteroinvasive properties on shigella sonnei. loss of this plasmid is accompanied by loss of the invasive phenotype as well as of the form i antigen expression. thirteen antibiotics belonging to different chemical families were used at subinhibitory concentrations to eliminate pwr105 from s. sonnei and escherichia coli k12. rifampicin, novobiocin, chloramphenicol, cotrimoxazole and erythromycin eliminated the plasmid from both strains. ... | 1986 | 3318870 |
| frequency of escherichia coli o157:h7 isolation from stool specimens. | during a 6-month period, 7252 faeces specimens were examined for escherichia coli serotype o157:h7. forty-nine specimens (0.7%) from 19 patients yielded this organism. escherichia coli o157:h7 was the third most frequently isolated bacterial pathogen, following campylobacter jejuni and (or) salmonella sp. although regional variation between laboratories determined whether campylobacter jejuni or salmonella was the primary bacterial pathogen isolated, e. coli o157:h7 was consistently isolated mor ... | 1987 | 3319115 |
| pathogenesis of shigella infections. | | 1987 | 3321018 |
| inhibition of enteropathogenic escherichia coli (strain rdec-1) adherence to rabbit intestinal brush borders by milk immune secretory immunoglobulin a. | | 1987 | 3321942 |
| enhancement of the secretory iga response to enteropathogens. | | 1987 | 3321943 |
| plasmid-mediated contact haemolytic activity in shigella species: correlation with penetration into hela cells. | the main feature of virulent strains of shigella is their ability to invade eucaryotic cells. this phenotype is both plasmid-mediated and temperature-regulated. in the present report, we demonstrate a plasmid-mediated and temperature-regulated haemolytic activity in s. flexneri, s. dysenteriae and s. sonnei. detection of this haemolytic activity requires centrifugation of suspensions containing bacteria and erythrocytes, followed by incubation of the pellets at 37 degrees c. no soluble intra- or ... | 1986 | 3322171 |
| invasion of epithelial cells by shigellae. | | 1986 | 3322174 |
| plasmid-mediated intracellular multiplication of shigella flexneri. | | 1986 | 3322175 |
| synthetic peptides of shiga toxin b-subunit induce antibodies which neutralize the biological activities of the toxin. | | 1986 | 3322336 |
| assessment of antibody screening in selection of subjects for field trials of shigella vaccines. | | 1986 | 3322338 |
| clinical and field trials to assess the efficacy of vaccines against bacterial enteric infections. | | 1986 | 3322343 |
| evaluation of rapid sys system as screen for yersinia enterocolitica in the united states. | clinical isolates (n = 150) from stool specimens were selected for evaluation of the rapid sys system (analytab products, plainview, n.y.) as a screening test for shigella spp., yersinia enterocolitica, and salmonella spp. the gram-negative identification card (vitek systems, inc., hazelwood, mo.) was used for identification. although acceptable performance of the rapid sys system was described, the interpretative criteria provided by the vendor for previous studies led to inappropriate screenin ... | 1987 | 3323232 |
| [action of the lactoperoxidase system on salmonellae and shigellae]. | in vitro experiments have demonstrated that the lactoperoxidase system produces a bactericidal effect on salmonellae and shigellae. the physiological concentrations of the components of this system, making it possible to obtain a pronounced bactericidal effect, have been established. lactobacilli have been shown to potentiate the effect of the lactoperoxidase system. the possibility of realizing the bactericidal properties of the lactoperoxidase system with respect to salmonellae and shigellae i ... | 1987 | 3324588 |
| microbiological perspectives of co-trimoxazole. | trimethoprim-sulfamethoxazole in vitro activity was compared with ampicillin, tetracycline, sulfonamide and trimethoprim against isolates of 24 gram-negative and 11 gram-positive species. the incidence of more than 10% of strains with minimal inhibitory concentrations above 32 mg/l was restricted to escherichia coli, shigella spp., klebsiella pneumoniae, providencia rettgeri, morganella morganii, methicillin-resistant staphylococcus aureus and staphylococcus epidermidis. occasionally, streptococ ... | 1987 | 3325434 |
| [economic loss from dysentery combined with ascariasis and trichocephaliasis]. | | 1987 | 3325790 |
| [the role of 4-quinolones and antibiotics in the elimination of virulence plasmids of enterobacteriaceae]. | twenty-six antibiotics belonging to thirteen different chemical families had been tested on seven enterobacteriaceae harbouring a virulence plasmid (shigella sonnei, s. flexneri, s. dysenteriae, escherichia coli (two plasmids). yersinia and salmonella dublin). novobiocin and rifampicin were found to be most efficient eliminating three plasmids of which two come from shigella. clindamycin, cotrimoxazole, nifurzide, ciprofloxacin, and tilbroquinol were also efficient, but at lower rate. four virul ... | 1987 | 3325901 |
| [reactive arthritis in enteral infections]. | certain gastrointestinal tract infections can trigger reactive arthritis. the best known triggering agents are yersinia, salmonella, shigella, campylobacter jejuni, and possibly clostridium difficile. the clinical findings show a subacute onset of mono- or oligoarthritis, less often of asymmetric polyarthritis with predominance of the lower limbs. the clinical picture is somewhat modified by the genetic constitution (hla type) of the patient. hla-b27-positive patients appear to have more severe ... | 1987 | 3326361 |
| igg response of dysenteric patients to antigens coded by the virulence plasmid of enteroinvasive pathogens. | igg antibody response to antigens coded by the virulence plasmid of enteroinvasive pathogens was studied in dysenteric patients by elisa. a plasmidless escherichia coli k-12 strain and its transconjugant harbouring the 140 megadalton plasmid of a virulent e. coli 0124 strain were used as antigens. sera of 12 dysenteric patients taken on the first, third an twelfth week after shigella sonnei infection were examined. for comparison sera of 23 nondysenteric persons were also applied. titres of the ... | 1987 | 3326389 |
| effect of outer membrane proteins of shigella on delayed hypersensitivity in mice to sheep red blood cells. | small doses of outer membrane proteins (omp) of shigella flexneri injected intraperitoneally into mice 1 to 3 days before or 3 days after sensitization of animals with sheep erythrocytes were found to increase delayed hypersensitivity as measured by the footpad reaction. in contrast, administration of higher doses of omp resulted in suppression of hypersensitivity response. cell transfer experiments showed that the spleen cells from sensitized and omp treated mice transferred stimulating and sup ... | 1987 | 3326545 |
| multiple drug-resistance in shigella flexneri isolated from a patient with human immunodeficiency virus. | persistent shigellosis, due to shigella flexneri resistant to multiple antibiotics, developed in a 40-yr-old homosexual man with human immunodeficiency virus infection. the shigella strain demonstrated resistance to ampicillin, tetracycline, and trimethoprim-sulfamethoxazole. although shigella flexneri isolates resistant to trimethoprim-sulfamethoxazole are uncommon in the united states, laboratories should monitor resistance patterns through routine in vitro susceptibility testing. | 1987 | 3326711 |
| differential clinical features and stool findings in shigellosis and amoebic dysentery. | to obtain information that could assist the clinician to differentiate between shigellosis and amoebic dysentery, we compared clinical features and stool findings in 58 adult male patients in bangladesh. mean values indicated that patients with invasive amoebiasis were older and had a longer prehospital illness, a lower body weight, less frequent fever, a lower haematocrit and a higher white blood cell count than patients with shigellosis. the mean number of faecal leucocytes per mm3 was signifi ... | 1987 | 3328339 |
| the aetiology of diarrhoea in a farming community in zimbabwe. | a survey of enteric pathogens in 143 children with diarrhoea, 116 age-matched controls and 524 asymptomatic people of all ages in a commercial farming area was carried out over a period of one year. giardia lamblia was detected in 34% of the cases, 29% of the controls and 23% of asymptomatic people of all ages. various bacterial pathogens (campylobacter, shigella, salmonella and enteropathogenic escherichia coli) were isolated from 23% of the cases and 15% of the controls. enteropathogenic e. co ... | 1987 | 3328340 |
| [heterogeneity of shigella flexneri during selective synchronization]. | the study of a number of main growth characteristics of s. flexneri (s) cells during the first and second cycles of their cultivation in a solid culture medium in peshkov's chamber from seed material, nonsynchronized and synchronized by c. e. helmstetter's method, has revealed that this method does not permit obtaining completely synchronized cultures. four physiological cell clusters, differing in the time of generation, growth rate, size at the beginning and the end of the cycle and the time o ... | 1987 | 3328453 |
| [antipuretic, antimicrobic action of gegen qinlian tang]. | | 1987 | 3328655 |