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salmonella phage glycanases: substrate specificity of the phage p22 endo-rhamnosidase.interaction between phage p22 and phenol-water extracted lipopolysaccharides from sensitive salmonella bacteria belonging to serogroups a, b and di results in hydrolysis of the alpha-l-rhamnosyl linkages within the tetrasaccharide repeating unit of the o-antigenic polysaccharide chain. these o-antigens have identical structures except for the nature of the 3,6-dideoxy-hexosyl group linked to o-3 of the d-mannosyl residue. removal of the dideoxysugar, or periodate oxidation followed by borohydrid ...1979383902
cyclacillin: in vitro activity against aerobic and anaerobic bacteria.cyclacillin at 50 microgram/ml inhibited streptococci (100%), shigella (72%), and bacteroides (b) fragilis ss fragilis (84%). at 100 microgram/ml it inhibited proteus (75%), b. fragilis (86%), and e. coli (50%). cyclacillin was more effective than ampicillin against beta-lactamase-producing h. influenzae. the activity of cyclacillin was found to be more enhanced when tested in nutrient agar and mueller-hinton agar than in brain heart infusion agar.1979383419
enterotoxin production by shigella flexneri type 2a, strain no. m42-43.enterotoxin produced by shigella flexneri type 2a, strain m42-43, is similar to "shig-like" cytotoxic enterotoxin and shares common features with that of other s. flexneri strains. on the basis of molecular filtration and neutralization experiments it is suggested that the same molecule carries these biological characters.1978382758
[diagnosis of acute appendicitis in patients suffering from diarrhea]. 1979382628
shigella infection of henle intestinal epithelial cells: role of the bacterium.epithelial cell infection by shigella flexneri 2a was studied in an in vitro model system. using the henle 407 human intestinal epithelial cell line as host cells, a standardized experimental protocol which allowed quantitative measurement of infection was developed. intravellular residence of infecting organisms was confirmed by indirect fluorescent-antibody staining of unfixed and methanol-fixed (henle 407) cells and by quantitative bacteriological culture of disrupted host cells after infecti ...1979381204
somatic antigens of shigella. the structure of the specific polysaccharide of shigella newcastle (sh. flexneri type 6) lipopolysaccharide.the specific polysaccharide was obtained from the lipopolysaccharide of shigella newcastle by mild acid hydrolysis and further purified by permeation chromatography on sephadex g-50. it was found to consist of l-rhamnose, 2-acetamido-2-deoxy-d-galactose, d-galacturonic acid residues and o-acetyl groups in the molar ratios of 2:1:1:1. on the basis of 1h and 13c nuclear magnetic resonance spectroscopy, methylation analysis, partial acid hydrolysis, smith degradation, and chromium trioxide oxidatio ...1979381001
age-dependent macrophage functions in new zealand black mice. 1979380820
study on the possible entry of bacterial endotoxin and prostaglandin e2 into the central nervous system from the blood.1 a study has been made of the possible entry of 51cr-bacterial endotoxin and [5,6,8,11,12,14,15(n)-3h]-prostaglandin e2 ([3h5-pge2) into the cns of the anaesthetized cat. 2 no radioactivity was detected in perfusates of the preoptic-anterior hypothalamus or in the cerebrospinal fluid (c.s.f.) in vivo, or in brain tissue post mortem following intracarotid infusion of 51cr-bacterial endotoxin. 3 intracarotid administration of [3h]-pge2 resulted in the entry of radioactivity into the cns of endoto ...1979380709
[course of dysentery and the specific alteration of the neutrophils in children in the use of monomycin and a diet with an increased protein content].acute dysentery mainly due to shigella sonnei and dynamics of the neutrophil damage index (ndi) of the blood were studied in 80 children at the age of 1 to 14. 20 children (group 1) were treated with monomycin under conditions of a diet with an excessive content of protein (by 25% higher than the physiological norm). 60 children or 3 groups of 20 children each were not subject to such conditions and were considered as control groups. reduction in the time of recovery (by 4 days) and bacteriologi ...1979380456
[data to substantiate the early immunological diagnosis of dysentery].the interaction of the whole blood from patients with dysentery and gastrointestinal diseases of non-dysenteric etiology, with the causative agents of dysentery, sh. sonnei and sh. flexneri, and saprophytic, staphylococci labeled with radioactive isotopes was studied in vitro. in dysentery an increase in the capacity of the blood for shigella fixation was observed from the beginning of the disease. during the 1st week of the disease this reaction was strictly specific and accompanied by a decre ...1979380241
[sh. flexneri population heterogeneity according to the cellular fatty acid makeup].the composition of fatty acids in the cells of 100 clones of sh. flexneri, strain 9054, isolated from a dysentery patient was studied by the method of gas chromatography. fatty acid composition in 75 clones was similar to the fatty acid composition of the initial culture: 25 clones differed to a variable degree from the prevailing group of clones in the content of unsaturated and cyclopropane fatty acids and, to a considerably lesser extent, in the content of palmitic acid and a number of other ...1979380239
[unclassified colicinogenic types of sh. sonnei]. 1979380151
[effect of metabolism products of shigella sonnei, sh. flexneri and sh. shigae on proteolytic activity of the organism connective tissue].the activity of proteolytic enzymes, neutral proteinases and cathepsins e+d, was determined in homogenates from the tissues of capsules formed around diffusion chambers with shigella (shigella sonnei, sh. flexneri, sh. shigae) 1, 2, 3, 5 and 10 days after their implantation to the abdominal cavity of rabbits. the specific proteolytic activity in the capsule tissues in the experimental variants is higher in all the studied periods than in the control and its singularities depend on the microbe sp ...1979380094
[a dysentery epidemic among tunisia travelers]. 1979379657
[immunogenic dna of shigella sonnei bacteriophage].immunogenic and some other properties of sh, sonnei "ufa" phage dna were investigated. this dna has been shown to possess high immunogenic activity indicating the presence of an unusual base. the base is distinct from 5-omc, glucosylated 5-omc, 5-omu or dihydroxypentyluracil. the phage dna is double stranded and has the molecular weight of 1.11.10(8) daltons and tm=85.5 degrees c. base sequences common with t4 dna have been found using the competition dna-dna hybridization technique. experiments ...1977379597
monocyte function in psoriasis.monocytes derived from the peripheral blood of psoriatic patients demonstrated a significantly higher phagocytic capacity (36 to 40%) for both 125i-labeled shigella flexneri and 125i-labeled staphylococcus albus compared with monocytes from healthy subjects. monocytes from psoriatic patients showed a 2-to-4fold increase in bactericidal capacity against s. albus when compared with normal monocytes. however, the bactericidal capacity of monocytes from diphylline-treated patients did not differ fro ...1979379240
achromobacter species (cdc group vd): morphological and biochemical characterization.twenty-three isolates of achromobacter species (cdc group vd) were examined morphologically and biochemically. gram stains revealed gram-variable bacilli frequently curved or hooked at one pole and often coryneform in shape and arrangement. electron microscopy revealed the presence of extracellular material in polar accumulations and demonstrated the polar flagella arrangement seen by light microscopy to be lateral. two colony types were produced; one was minute and watery at 24 h (35 degrees c) ...1979379035
molecular characterization of plasmids from virulent and spontaneously occurring avirulent colonial variants of shigella flexneri.spontaneous transition of shigella flexneri from t- to o-type colonies with concomitant loss of virulence dose not appear to be accompanied by a change in any of the four plasmic species found in the virulent parent.1979378861
enzyme-linked immunosorbent assay for immunoglobulin g and immunoglobulin a antibodies to shigella flexneri antigens.an enzyme-linked immunosorbent assay has been developed to detect class-specific antibodies to shigella flexneri lipopolysaccharide antigens. this enzyme-linked immunosorbent assay system has been used to measure antibodies present in serum or intestinal secretions without further purification. it is considerably more sensitive than passive hemagglutination, allowing detection of as little as 1.3 ng of specific immunoglobulin g antibody per ml in immune sera. optimal conditions for this assay ar ...1979378853
vivo clearance of enteric bacteria from the hemolymph of the hard clam and the american oyster.american oysters, crassostrea virginica, and hard clams, mercenaria mercenaria, were experimentally contaminated with escherichia coli, salmonella typhimurium, and shigella flexneri either by intracardial injection or via the natural route of ingestion. bacterial inactivation in the hemolymph was monitored for 72 h after exposure to these enteric pathogens at 20 and 6 degrees c. at 6 degrees c, both mean bacterial uptake by ingestion and subsequent clearance was singificantly lower that at 20 de ...1979378127
[level and nature of the drug resistance of shigella flexneri].characteristics of antibiotic resistance of 300 strains of shigella flexner 2a isolated from patients within 1976--1977 in the regions where these bacteria were very rare for a long period of time were studied. it was shown that most of the isolates were resistant to chloramphenicol (88.1 per cent), tetracycline (94.8 per cent), streptomycin (89.1 per cent), polymyxin m (82.4 per cent) and others. 46.5--61.6 per cent of the isolates were resistant to neomycin antibiotics. a high level of the res ...1979378114
[dynamics of the change in sensitivity of shigellae to gentamycin and cephaloridine in vitro].the dynamics of the changes in the shigella sensitivity to gentamicin and cephaloridin was studied in vitro using liquid nutrient media with gradually increasing concentrations of the drugs. 50 passages were performed. it was found that shigella flexner and sonnei decreased their sensitivity to gentamicin to a little extent and remained middle sensitive. sensitivity of shigella flexner to cephaloridin also changed to a little extent, while shigella sonnei became moderately resistant.1979378113
[use of india ink for the purpose of the specific detection of pathogenic microorganisms in histological preparations]. 1979377872
[search for host specificity systems in shigella using dd-series phages].a total of 712 shigella strains were studied with the use of dysentery diagnostic phages dd ii, dd vi and dd vii in order to reveal the systems of host dna specificity. the study comprised 4 tests: mass screening by the intensity of phagolytic reaction of phages in various strains; and the determination of the parameters of adsorption. as a result, an effective modification and restriction systems were revealed in sh. sonnei 311 with the use of phage dd ii. bacteriophage dd vii was effectively r ...1979377869
[analysis of merodiploid strains of sh. flexneri that have lost virulence].episome f'13 introduced into the genome of a virulent sh. flexneri strain brought about changes in a number of properties of the recipient strain. the expression of these properties was not connected with the chromosome area allelic to the plasmid genome. these changes seem to be induced by the mobilization of the chromosome genes of e. coli. the loss of virulence in sh. flexneri strains carrying episome f'13 seemed to be the consequence of two reasons: the overlapping of kcpa gene by its domina ...1979377868
[patterns determining the dynamics of the epidemic process in sonne dysentery and its relation to the type of causative agent].the results of studying the dynamics of the epidemic process of dysentery, based on the data from 3 districts of moscow, are presented. the study revealed the periodicity of 3 years in the course of the epidemic process of dysentery, occurring against the background of a considerable predominance of sh. sonnei, biochemical type 2. the use of the cohort method for analyzing the age structure of dysentery cases showed the possibility of using this method to find out to what extent the epidemic pro ...1979377866
[parenteral use of a therapeutic vaccine in acute dysentery]. 1979377515
[an epidemic of shigella sonnei in a swedish un battalion]. 1979376980
[storage of shigella flexneri with virulence preserved]. 1979376977
shigella serotypes in recent isolations at lucknow. 1979376439
corneal ulceration secondary to intestinal shigella sonnei.a 34-month-old boy had a corneal ulcer due to shigella sonnei. to my knowledge, this is the first documented case reported where the same organism was recovered from the stool. one other case of s sonnei and two cases of s flexneri corneal ulcers have appeared in the literature. all cases have been in young black boys between the ages of 23 months and 6 years. the exact pathogenesis remains unclear, but direct fecalocular inoculation into a preexisting epithelial defect, a probable herpetic dend ...1979375886
[interrelationship between the serum lysozyme level and the leukocyte count in sonne and flexner dysenteries].interrelation between the level of the serum lysozyme and the count of the leucocytes, as well as the severity of the disease was found in testing of the peripheral blood of 133 patients with acute sonnei and flexner dysentery. it is recommended to use the lysozyme index in the clinical practice as one of the additional criteria for estimation of the severity of acute dysentery.1979375827
enteric gram-negative rods, 1976. 1979375729
[biological preparations in the overall therapy of children convalescing after dysentery].in studying the state of microbial population in the intestine of children after dysentery dysbacteriosis was revealed in 72.7% of cases. use of biological preparations normalizing microbial population in the intestine (colibacterin) in 859 children reduced the incidence of protracted forms of the disease and of repeated excretion of the causative agent in comparison with the control group (785 children).1979375635
[conjugative r plasmids of enterobacteria]. 1979375629
bacteremia caused by shigella flexneri. 1979375454
bacteriophage specificity in the identification of yersinia pestis as compared with other enterobacteria.bacteriophage typing of yersinia pestis and the specificity of the phage among enterobacteriaceae were investigated. the bacteriophage used for rapid identification of y. pestis reacted with representative strains of all recognized species of shigella as well as with salmonella cholerae-suis. reactive shigella serotypes were sh. dysenteriae 1 and 9, sh. flexneri 2a, sh. boydii 1 and 6, and sh. sonnei. patterns consisting of isolated plaques (two cases) or absence of plaques were observed when th ...1978375327
shigella vaginitis: report of 38 patients and review of the literature.thirty-eight cases of shigella vaginitis were identified in a retrospective review of records kept over the past 14 years. the cases of vaginitis were due to three subgroups of shigella. vaginitis varied in severity and duration, persisting for several months in some instances. in 47% of the cases, there was associated bloody vaginal discharge. only two children had diarrhea temporally associated with vaginitis, but six others had had diarrhea. many treatment modalities were used. systemic antib ...1979375177
non-random distribution of transduction termini in transductants from the integrated r plasmid, r100-1.tra+ and tra- derivatives of drug resistance plasmid, r100-1, were isolated by phage p1 from an hfr donor with integrated r100-1 and then analyzed by complementation tests with tra- point mutants of flac. tra+ derivatives of r100-1 carrying tetracycline resistance alone and those carrying all six drug-resistrance genes could support transfer of tra- point mutants of flac except flac traj, whereas all of tra- derivatives of r100-1 failed to complement any one of tra- point mutants of flac. this s ...1979375027
macrophage fatty acid composition and phagocytosis: effect of unsaturation on cellular phagocytic activity.in order to manipulate the physical properties of the macrophages membrane, methods were developed which potentiated the incorporation of exogenously supplied fatty acids into membrane lipids. chromatograms of macrophages which were grown in the presence of a variety of fatty acids demonstrated that exogenously supplied unsaturated fatty acids (palmitoleic, oleic, elaidic, linoleic, linolenic and arachidonic acids) were readily incorporated into the cells and selectively altered the fatty acyl c ...1979374248
studies on virulence of shigella flexneri.the antigenic structure and some biological properties were compared in virulent s. flexneri strains of differences in: 1) antigenic composition; 2) biochemical activity; 3) sensitivity to a set of shigella phages and 4) susceptibility to phagocytosis. the only difference found concerned the ld50 for mice; it was 10 to 100 times larger for avirulent than for virulent strains of s. flexneri. the toxic products of the strains were also compared. the lipopolisaccharide and free endotoxin purified f ...1978373685
immune processes induced by dysentery bacilli in mice.studies on immune reactions induced by dysentery bacilli revealed that mice immunized orally or intravenously develop the specific general resistance against reinfection with the microorganisms. antibodies circulating against bacilli were the indicators of humoral immune response and the activated t lymphocyte detected in the spleen of immunized mice were the indicators of the cell mediated immunity.1978373684
interferon induction in mice by uniform salt forms of shigella sonnei lipopolysaccharide and its components.interferon-inducing capacity of uniform salts of shigella sonnei lipopolysaccharide (lps), its polysaccharide component and lipid a were compared. low-molecular-weight triethylamine and ethanolamine salt forms of the lipopolysaccharide were most active in interferon production. it was confirmed that lipid part of lps is responsible for interferon-inducing activity.1978373682
[antibiotic resistance of shigella in different regions of the ussr].shigella were most sensitive to polymyxin ceporin, ampicillin, neomycin and furazolidone and resistant to chloramphenicol, tetracycline and streptomycin. shigella resistant simultaneously to two or three drugs mainly to tetracycline + chloramphenicol, tetracycline + streptomycin and tetracycline + chloramphenicol + streptomycin were most frequent. the frequency of the shigella strains carrying r-plasmids increased from 28 per cent in 1969--1970 to 72.6 per cent in 1977. the shigella strains isol ...1979373617
[comparative evaluation of different globulin preparations and of the means for blood sensitization in obtaining antibody erythrocytic diagnostica].in comparing the hemosensitizing activity of various immunoglobulin preparations with different methods of erythrocyte loading the greatest activity and specificity was revealed in igg isolated by means of deae-sephadex a-50. the method of erythrocyte sensitization with the use of alizarin blue indicator was more advantageous in respect to sensitivity and specificity of sh. sonnei and newcastle indication over boyden's, jandl and simmons', bing's methods.1979373342
[properties of the r plasmids of sh. flexneri strains isolated in bulgaria].the study of 3,237 sh. flexneri strains isolated in bulgaria revealed that 50.72% of them were resistant to antibiotics. 95.88% of the antibiotic-resistant strains were found to have r factor of incompatibility groups f, i or n. the plasmids of incompatibility group f had the property fin+, and the plasmids of groups i and n had the property fin-. r plasmids were divided into 4 groups by their ability for phage restriction.1979373338
[electron microscopic study of experimental infection. ii. a study of the dynamics of shigella infection].the dynamics of experimental shigella infection of chick embryo fibroblasts was studied with the use of electron microscopy. the antibiotic-resistant forms of sh. sonnei 1,188 was found to be incapable of invasion into the fibroblast cytoplasm and intracellular proliferation. the destruction of fibroblasts observed during the infection was seemingly caused by the action of bacterial endotoxins.1979373336
[cellular immunity factors in dysentery].cellular immune response was studied in 89 adult patients suffering from various clinical forms of acute dysentery, with the use of the lymphocyte blast-cell transformation reaction under the action of a specific antigen (dysenterin) and a nonspecific mitogen (phitohemagglutinin). functional value of t-lymphocytes proved to be retained in patients with acute dysentery; there was also lymphocyte stimulation by a specific antigen in patients with moderately severe and severe forms of dysentery dur ...1979373334
[system of host specificity and the dna methylases of shigellae and their phages].in shigella sonnei cells there is a host dna specificity system responsible for modification and restriction of ddii phage. dna methylase from shigella stutzeri cells is specific for adenine and catalyses the appearance of 6-'-methylaminopurine in the acceptory dna. methylases from shigella sonnei cells are specific for adenine and cytosine and provide for the presence of 6'-methylaminopurine and 5'-methylcytosine in dna. the modifying activity of these cells may be equally likely associated wit ...1978373252
determination of very thin pili by the bacterial drug resistance plasmid r485. 1978372971
efficient transfer of conjugative plasmids by multipoint inoculation and some observations on host range and prevalence of r plasmids. 1978372969
[chimpanzee as a pet: cause of a shigella infection]. 1979372831
[shigellosis]. 1979372830
invasive strain of escherichia coli belonging to o group 29.a strain of escherichia coli isolated from a child with diarrhea was biochemically similar and antigenically identical (o and k antigens) to the standard strain of e. coli o group 29 and was positive in the serény test for invasiveness, which suggests that it can cause a shigella-like illness.1979372230
the effect of endotoxin-induced intraocular inflammation on the rat lens epithelium.intraocular inflammation induced by an intravitreal injection of shigella endotoxin into the rat eye produces early changes in the number of dividing cells of the lens epithelium and affects the organization of the meridional rows. a depression in mitotic activity in the germinative zone is observed during the first 24 hr after injection. at 48 hr, despite the continued mitotic inhibition in the germinative zone, an increase in cell division occurs in the central zone. by 72 hr, the germinative ...1979372124
construction of plasmid cloning vehicles that promote gene expression from the bacteriophage lambda pl promoter.two multiple-copy, cole1-type, plasmid cloning vehicles, phub2 and phub4, have been constructed that carry four different single restriction sites down-stream from the phage lambda promoter pl. the promoting activity of pl is switched off at low temperature in the presence of a cits gene that specifies a temperature-sensitive repressor but could be activated by heat induction. cits was located either on the host chromosome, or on a second plasmid prk248 that is compatible with the cloning vehicl ...1979372049
[vulvovaginitis and dysentery (author's transl)]. 1979371835
structural studies on the hexose region of the enterobacteriaceae type r3 core polysaccharide.the structure of the hexose region of the enterobacteriaceae type r3 core polysaccharide, which is the common core in shigella flexneri and some escherichia coli lipopolysaccharides (lps), has been investigated. the principal method used in these studies involved methylation analysis of the original and modified materials. the polysaccharide was modified by n-deacetylation-deamination and by smith degradation. as a result of these studies, the following structure is proposed: alpha-d-glcp-(1 lea ...1979371805
[role of epithelial basement membrane of the small intestine in providing immunologic homeostasis].a morphometric method was used experimentally to prove the immune barrier function of the epithelial basal membrane of the small intestine mucous membrane. the epithelial basal membrane located on the border of the epithelium and connective tissue provides biochemical and immunological protection of the layer proper against penetrotion into it of antigens from the epithelium, being a barrier of immunological homeostasis. the damage of the epithelial membrane is accompanied by coming of the intes ...1979371587
[nomenclature of the bacteria of the genus shigella and their systematic position in the family of enterobacteriaceae]. 1978371309
[content of a-, g- and m-class immunoglobulins in adults perorally immunized with a liver sonne dysentery vaccine].the authors studied the immunological shifts in the blood and saliva of 357 adults immunized with live enteral dysentery sonne vaccine from a spontaneous mutant according to different schemes (1, 2, 3, 4 injections at 2--3-day intervals. the general level of iga, igg, and igm increased during the immunization; there was also an elevation of the specific antibodies level of these classes in the blood of the persons vaccinated. accretion was the greatest of iga both in the blood and in saliva of t ...1978371307
[gibbs free energy in periodic enterobacterial cultures].the authors present the results of study of the gibbs' free energy (--delta g) output in periodic cultures of nonpathogenic and enteropathogenic escherichia, shigellae, salmonellae, proteus, serratia, and klebsiellae in the hydrolysate broth in a special twelve--chamber cultivator. graphic expression--deltag = f(n) permitted to reveal differences in the energy characteristics of individual enterobacteria genera and species. it is supposed that such studies could be useful in the choice of redox ...1978371306
[indices of peripheral blood lymphocyte blast and macrophagal transformation in typhoid and dysentery in the presence of a bacteriophage homologous to the type of causative agent]. 1978371298
[evaluation of the diagnostic effectiveness shigella sonnei and shigells flexneri antigenic preparations by serological examination data on patients with clinical dysentery]. 1978371292
[media with yeast rna for isolating shigella flexneri from feces].the authors demonstrated a considerable, in comparison with the control, increase of the biomass of sh. flexneri causative agent, when growing in meat-peptone broth with 1-4 mg of narna, and of the number of colonies in the feces of the patients and of healthy persons artificially contaminated with sh. flexneri when inoculated in ploskirev's agar or endo medium with 4 mg/ml of narna. using media with narna for laboratory diagnosis of dysentery is recommended.1978371291
[characteristics of the action of the biologically active factor from virulent shigella flexneri strains in a study on mice and a cell culture].the authors subjected to further study the biologically active factor revealed by them earlier in the virulent sh. flexneri cultures by using the genetically bound triad of sh. flexneri 5a-222 cultures and the corresponding couple of sh. flexneri 2a-516. there was shown correlation of the strains virulence determined by the keratoconjunctival test, with the presence of genetically-determined production of the biologically active factor detectable in the culture filtrate, which produced toxic act ...1978371290
[effectiveness of using a lyophilized erythocyte antibody diagnosticum and other methods in sonne dysentery]. 1978371282
[production of auxotrophic mutants of shigella sonnei].use of selection method with r. ffm phage offered a possibility of obtaining r-forms of sh. sonnei from the population of bacteria of phase ii with a low frequency of natural dissociation. mono- and polyauxotrophic mutant of i, ii and r-phases by hir, ilv, pro, and mtl markers were obtained by treatment of sh. sonnei strains with nitrosoguanidine and ethyl-methanesulfonate. the frequency of mutation in two sh. sonnei strains studied in this work (ubc and no. 941) by the same genetic regions prov ...1978371278
[distribution of pathogenic enterobacteria in the water of open reservoirs].investigations carried out indicated that the main source of microbial contamination of open reservoirs were effluents often drained without any purification and disinfection. salmonellae were isolated in 10.6 +/- 0.88% of the water samples examined in the southern regions of the country. isolation of pathogenic enterobacteria from the water of open reservoirs was seasonal, with the maximal percentage in winter and in spring, this being connected with delay of self-purification processes in the ...1979371266
[various epidemiologic characteristics of dysentery]. 1979371138
[role of histamine in the development of inflammation of the large intestine mucosa in acute dysentery]. 1979370753
the transfer of antibiotic resistance by r plasmids in shigellae.forty-five strains of shigella species isolated at auckland hospital from 1972--1974 were investigated for antibiotic resistance. forty-two strains were resistant to one or more antibiotics and 11 strains transferred antibiotic resistance by conjugation to e. coli.1978370696
thermosensitive h1 plasmids determining citrate utilization.twelve thermosensitive h1 plasmids from strains of salmonella typhi that had caused outbreaks of chloramphenicol-resistant typhoid fever in vietnam, thailand and india mediated citrate utilization (cit+) in a prototrophic escherichia coli k12 strain but not in the s. typhi strains from which they were derived. four h1 plasmids from a similar outbreak in mexico differed from the far eastern plasmids in not mediating citrate utlization but in mediating mercury resistance. h1 plasmids resembling th ...1978370344
molecular characteristic of antibodies in the blood serum and in the lymph nodes during fractional immunization with small doses of bacterial vaccines.an experiment on 58 rabbits demonstrated that only macromolecular agglutinins 19s are present in extracts from the lymph nodes after fractional immunization with small doses of typhoid vaccine or some dysentery vaccine. at the same time, antibodies in the blood serum are distributed in fractions of different molecular weight, starting from the 14th day of experiment. it is very probable that this distribution of antibodies is associated with the occurrence in the blood of an inhibitor of the act ...1978370307
characteristics of lipid a-protein complex from endotoxin of shigella dysenteriae type 1 (s and r strains).mild acetic acid hydrolysis of endotoxin (lipopolysaccharide-protein complex) of shigella dysenteriae type 1 (s and r forms) yielded a lipid a-protein complex that consisted of amino acids, fatty acids, and sugar and, in terms of chemical composition, displayed no marked differences between the s and r forms. its protein portion (53 to 56%) consisted of at least 16 amino acids. in the fatty acid portion (14 to 18%), myristic, 3-hydroxymyristic, palmitic, and stearic acids accounted for 50%. the ...1979370018
hemagglutination patterns of enterotoxigenic and enteropathogenic escherichia coli determined with human, bovine, chicken, and guinea pig erythrocytes in the presence and absence of mannose.a hemagglutination (ha)-typing system has been developed for the presumptive identification of enterotoxigenic escherichia coli (etec) possessing the colonization factor antigens (cfa) cfa/i or cfa/ii. e. coli isolates are grown on cfa agar and tested for mannose-sensitive (ms) or mannose-resistant (mr) ha of human, bovine, chicken, and guinea pig erythrocytes. cfa/i-positive etec exhibit mrha with human, bovine, and chicken erythrocytes, but no ha with guinea pig erythrocytes. cfa/ii-positive e ...1979370014
mitomycin c stimulates production of a toxin in shigella species that causes morphological changes in chinese hamster ovary cells.shigella dysenteriae produced a toxin that caused morphological changes in chinese hamster ovary cells which was different from the neurotoxin (y. takeda, k. okamoto, and t. miwatani, infect. immun. 18:546--548, 1977). it was found that the toxin was produced not only by s. dysenteriae but also by s. flexneri, s. boydii, and s. sonnei. the production of the toxin was stimulated by the addition of 1 microgram of mitomycin c per ml to the culture in the early logarithmic growth phase.1979370009
specificity and functions of guanine methylase of shigella sonnei ddvi phage.dna methylase methylating adenine with formation of 6-methylaminopurine has been identified in shigella sonnei 1188 cells which are the natural host of ddvi phage. at the same time, in dna of ddvi phage replicating both in sh. sonnei 1188 cells and in escherichia coli b cells 7-methylguanine was found as the only minor base in amounts of 0.25 and 0.27 mol per 100 mol of nucleotides, respectively. the extract of the infected cells was found to contain both kinds of dna methylases: virus-specific ...1979369609
secretory iga antibodies to enterobacterial virulence antigens: their induction and possible relevance.1) milk and salivary s-iga antibodies are via the homing of iga producing cells from the peyer's patches closely connected with antigenic stimuli in the intestine. this explains the presence in human milk of s-iga antibodies against e. coli o and k antigens, v. cholerae and shigella o antigens, e. coli and v. cholerae enterotoxins. these secretory antibodies can be induced by intestinal exposure and boosted by parenteral vaccination. 2) preliminary data suggest that the iga response in the urina ...1978369311
the transport of specimen to the laboratory for the cultivation of pathogenic micro-organisms. ii. the shipment of faecal samples. 1977369267
[study of the enterotoxic action of the neurotoxin of the sonne dysentery microbe in a rabbit small intestine loop model].the enterotoxic action of neurotoxin from sonne dysentery microbes (obtained by the method of mesrobeanu et al.), and also of the culture autolysates and homologous boiven's endotoxin was studied on a model of the isolated loop of the rabbit small intestine. neurotoxin preparations obtained from virulent strains as well as autolysates of these cultures possessed enterotoxic activity, whereas purifed endotoxin preparations in doses of 1--10 mg failed to cause any dilatation of the isolated intest ...1978369252
[biological characteristics of transductants of shigella flexneri which have acquired different str-a-locus alleles]. 1978369250
[gibb's free energy as a index of the assimilation of carbohydrate sources by bacteria].the author presents substantiation of the use of thermodynamic function--of free gibbs' energy (--deltag) for energy characteristics of enterobacteria periodic cultures on synthetic media. examination of strains of e. coli k12p4x hfr, e. coli pa-309, and sh. newcastle 762s showed the --detlag = f (n) curves to characterise the energy value of various carbohydrate sources more completely, and also permitted the choice of their optimal concentrations in synthetic media for each of the enterobacter ...1978369248
[study of the mechanism of formation of protracted forms of dysentery]. 1978369138
[the serotypes of shigella in catania (italy) during 1974--1976 (author's transl)]. 1978367296
[incidence of intestinal disease due to yersinia enterocolitica (author's transl)].enteropathogenic bacteria have been identified in 413 of 7054 patients (5.9%) with intestinal disease who were examined at the institute of hygiene and microbiology, university of würzburg (south germany), during the period november 1975 to november 1977. salmonella was most frequently isolated (304 cases = 4.3%), followed by yersinia enterocolitica (102 cases = 1.5%). cases of shigellosis (7 cases) or infections with so-called enteropathogenic serogroups of escherichia coli (20 cases) were rare ...1978367003
[oral immunization with non-viable microorganisms or their antigens. 19. field trials with polyvalent enteritis-oralvaccines (author's transl)]. 1978367002
[immunoglobulins in acute dysentery]. 1978366890
[determination of shigella virulence on synchronized and nonsynchronized monolayers of hep-2]. 1978366345
[bacillary dysentery with recurrent spread of contagion at various levels]. 1979366302
patterns and mechanisms of antibiotic resistance. 1978366296
typing of shigella sonnei strains isolated in egypt. 1977366060
a comparative evaluation of media in the isolation of shigella dysenteriae type 1. 1978365721
[effect of leukocyte pyrogen on the phagocytic properties of macrophages in tissue culture].the effect of rabbit leukocytic pyrogen (lp) on the phagocytic activity of the peritoneal macrophages of albino mice against shigellae was investigated. the dose-effect dependence was revealed: high lp doses depressed the phagocytosis, and low ones were insufficiently effective; addition of average lp doses against the kanamycin background stimulated both the absorption phase and that of shigella digestion. phagocytosis stimulation with lp was also accompanied by an increase of the acid phosphat ...1978365272
effects of changes in mg++ ion concentration upon bacterial inhibition by beta-2-thienylalanine in defined media.variation in the inhibition of growth of escherichia coli and shigella sp. in various media containing beta-2-thienylalanine was attributed to differences in concentrations of mg++ ions. random blood samples 112 infants were tested for elevated phenylalanine in the phenylketonuria (pku) screening assay. magnesium ion levels also affected the results of this assay. at 0.05 g/1 mgso4, the concentration present in commerical pku test agar, four false positives were detected, while no readings could ...1978365154
salmonella serotypes encountered in animal feed additives in lebanon.animal feed-additive samples (n = 300) were examined for the presence of salmonellae, using the selenite-f broth-enrichment method followed by subculturing on salmonella-shigella and brilliant green agar with sulfadiazine selective agar plates. samples consisted of a variety of feed additives: 119 bone meal samples, 77 meat meal samples, 40 fish meal samples, and 64 miscellaneous meal samples. results of examination found 49 (41.2%) of the bone meal samples, 6 (7.8%) of the meat meal samples and ...1978365032
ecological control of the bacterial diarrheas: a scientific strategy. 1978364973
[quality of nutrient media according to indices of the cultivation process]. 1978364893
[immunochemical findings about the high molecular weight surface antigens of shigella sonnei].the authors studied immunochemical properties of the high molecular fraction of surface soluble antigens obtained by extraction with salt solutions from sh. sonnei (virulent strain 1041) dried with acetone. the high molecular fraction was isolated by gel-filtration on sepharose-4b. along with the o-somatic antigen, this fraction contained thermostable and thermolabile antigens resistant to trypsin and rna-ase treatment, and also protein-containing antigens disintegrated by trypsin. in difference ...1978364891
[experience using the klemparskaia-modified jerne technic for studying the immunogenicity of sh. sonnei]. 1978364888
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