Publications

TitleAbstractYear
Filter
PMID(sorted descending)
Filter
comparative in vitro antibacterial activity of ofloxacin and ciprofloxacin against some selected gram-positive and gram-negative isolates.the in vitro activities of ofloxacin and ciprofloxacin were tested against five selected groups of clinical bacterial isolates. both were active against: staphylococcus aureus and staphylococcus epidermidis, irrespective of their resistance to methicillin or gentamicin; haemophilus influenzae and neisseria gonorrhoeae, irrespective of their beta-lactamase production; members of the enterobacteriaceae family which were resistant to most oral beta-lactams, and most intestinal bacterial pathogens i ...19863469153
medical problems in ethiopian refugees airlifted to israel: experience in 131 patients admitted to a general hospital.within a 2 month period 131 ethiopian immigrants were admitted for treatment at a general hospital in jerusalem. there were 52 patients with malaria, 13 with typhoid fever, 24 with pneumonia, seven with tuberculosis, nine with shigella and 11 with campylobacter. over three-quarters of these patients were anaemic. in the majority of cases anaemia was normocytic and was most probably secondary to malaria and other intercurrent infections. the prevalence of diffuse non-toxic goitre was 7% in childr ...19863464761
childhood enteritis: a community study.the population aged 0-12 years living in the area of an italian local health unit (about 8,800 children) was studied for one year in order to estimate the overall incidence rate of enteritis and the incidence rates of diarrheas from bacterial agents. all children complaining of acute diarrhea and seen by the pediatric practices of the study area were recorded by date, age, and sex; the microbiological study of stools was performed for a sample of patients. all hospitalized cases of childhood ent ...19873449099
serological investigations for the cause of diarrhoea among patients and personnel in the minna and abeokuta hospitals/nigeria.188 blood samples were investigated for antibodies against bacteria causing diarrhoea in two hospitals in minna and abeokuta in nigeria. antibodies were found against entamoeba histolytica, yersinia enterocolitica, campylobacter jejuni, salmonella typhi, salmonella paratyphi c, shigella dysenteriae, shigella sonnei, shigella boydii, shigella flexneri and rotavirus.19873443752
investigations in the problem of diarrhoea in the melut district, south sudan (1981-1982).studies of diseases associated with diarrhoea in the melut district of south sudan in the years 1981-1982 showed amoebic dysentery and rota-virus to be predominate, whereby according to our previous results yersinia, campylobacter, shigella and salmonella infections also play a role. in addition other parasitological bacterial and viral-infections are presumed.19873443749
serological investigations on the cause of diarrhoea on the cape verde islands.in the serological tests 45.9% of all samples reacted positive against rota virus. 20.8% of samples revealed entamoeba histolytica positive sera, 25% shigella flexneri positive sera, 12.3% shigella sonnei and 13% shigella dysenteriae positive sera. campylobacter jejuni antibodies were found in 19% of samples, whereas 6.3% of sera reacted positive against yersinia enterocolitica type 3 and 4.8% against type 9.19873443746
two-hour miniaturized system for detection of enteropathogenic bacteria in stool cultures.a miniaturized 2-h system for detection of lactose-negative potentially enteropathogenic bacteria on the basis of nine enzymatic tests was evaluated. the system was challenged with 210 strains of lactose-negative and lactose-positive species grown on macconkey agar. specific and constant patterns were found for salmonella, shigella a-c, yersinia enterocolitica, aeromonas spp. and vibrio cholerae. shigella sonnei and hafnia alvei had an identical pattern, likewise plesiomonas shigelloides, haloph ...19873440458
cryptosporidial diarrhoea in children.by auramine and modified ziehl-neelsen staining, cryptosporidial oocysts were found in the stools of 31 (1.36%) out of 2,367 patients with diarrhoea. all specimens were also tested for salmonella, shigella, campylobacter, yersinia, and rotavirus. among these patients, 432 were children and 24 (5.5%) of them were positive for cryptosporidia. all children infected with cryptosporidia were immunocompetent. watery diarrhoea, vomiting and abdominal pain were the most frequent symptoms. the survey sho ...19873436676
media for the isolation of shigella. 19873429798
shigellosis in children. 19873428993
segmental necrotising enterocolitis: pathological and clinical features of 22 cases in bangladesh.to describe the pathology and clinical features of segmental necrotising enterocolitis (sne) in children and adults, 22 diarrhoeal patients (median age two years, range two months to 50 years) in bangladesh with this lesion detected at autopsy were examined and compared with two groups of diarrhoeal control patients. gross pathology consisted of purplish or black mucosal or transmural discoloration with erosions or ulcerations in segments of the jejunum or ileum of 18 cases and of the colon alon ...19873428668
a demographic survey of campylobacter, salmonella and shigella infections in england. a public health laboratory service survey.five laboratories serving a population of 1.5 million participated in a 2-year survey of campylobacter, salmonella and shigella infections in patients suffering from gastrointestinal symptoms. in total, 33,857 faecal specimens were examined of which 5.5% yielded campylobacters, 3.4% salmonellas and 0.8% shigellas; incidence of infection (per 100,000 population per year) was 58, 38 and 9 respectively. peak incidences occurred at different ages for each organism: campylobacter, 1-4 years (183); sa ...19873428371
[effects of loperamide on fecal electrolyte excretion in acute diarrhea in infants].the effect of loperamide (imodium) on fecal excretion of electrolytes was studied using a double-blind protocol in 63 infants aged 1 to 32 months, presenting with acute diarrhea. the etiology of these infections was due to virus (n = 9), e. coli (n = 4), salmonella (n = 4), shigella (n = 2) or was of undetermined origin (n = 34). the therapeutic protocol was the same for all the infants: ad libitum oral rehydration, with no drug being administered except for imodium at an average dose of 20 to 2 ...19873426370
[shigellosis in north rwanda 1982-1985]. 19873426312
correlation of the iga response in intestinal secretions with cellular events in the development of mucosal immunity. 19873425469
disseminated intravascular coagulation in post-dysenteric haemolytic uraemic syndrome.coagulation studies were carried out in 14 children with haemolytic uraemic syndrome that followed acute dysentery. stool cultures showed shigella dysenteriae in 3 cases and were sterile in the remainder. prolongation of the prothrombin time, activated partial thromboplastin time and thrombin time and raised levels of fibrinogen degradation products were found in 12 cases, indicating the presence of disseminated intravascular coagulation. renal histologic examination showed cortical necrosis in ...19873425309
acute diarrhoea in adults: aetiology, clinical appearance and therapeutic aspects.a prospective study of acute diarrhoea was performed during 15 months 1981/1982 and included 731 patients and 240 controls. 43% had been infected abroad. a cluster of travellers with bacterial pathogens was diagnosed in july-august. the following pathogens were found: campylobacter (18%), enterotoxigenic e. coli (6%), salmonella spp. (5%), rotavirus (4%), yersinia enterocolitica (3%), giardia lamblia (3%), shigella spp. (2%), clostridium difficile (2%), enteroviruses (2%) and entamoeba histolyti ...19883406670
occurrence of plesiomonas shigelloides in humans and water in zaria, nigeria.a total of 1,171 human faeces and 269 water samples were examined for plesiomonas shigelloides in zaria. the rate of isolation for humans was 0.17% while the detection rates in pond and well water samples were 7.4% and 0.6% respectively. no recoveries were made from water samples taken from streams, dams, tanks and taps. neither shigella nor salmonella was isolated from the water samples tested. it is concluded that water may play a role in the epidemiology of p. shigelloides but may not be a co ...19883405099
etiology of diarrhea among travelers and foreign residents in nepal.a bacterial pathogen was isolated from 47% of 328 expatriate patients with diarrhea seen at two medical clinics in nepal in 1986. enterotoxigenic escherichia coli (24%), shigella (14%), and campylobacter species (9%) were isolated most frequently. enteroinvasive and adherence factor-positive e coli were isolated from 2% and 1% of patients, respectively. giardia lamblia was detected in 12% of patients, rotavirus in 8%, and cryptosporidium and entamoeba histolytica each in 5%. blastocystis hominis ...19883404637
prospective study of diarrhoeal disease in a cohort of rural mexican children: incidence and isolated pathogens during the first two years of life.colonization of the intestine by putative pathogens was followed longitudinally in a cohort of 56 infants born during one calendar year in a rural mexican village with faecal cultures taken every fortnight and every time a child had diarrhoea. the frequency of isolation of pathogens during episodes of diarrhoea was compared with that of matched controls from the same cohort. incidence of diarrhoea during the first year of life was 98%, diminishing to 93% during the second year. the incidence cur ...19883402544
acute gastroenteritis: clinical features according to etiologic agents.the clinical manifestations in 595 children hospitalized with gastroenteritis during a 15-month time frame were studied. they were divided into eight groups according to etiologic agent: rotavirus (203 patients); salmonellae (98); escherichia coli (55); campylobacter (36); shigella (22); combined rotavirus and salmonellae (44); combined rotavirus and other bacteria (26); and no pathogen (111). the mean duration of diarrhea was shortest in the rotavirus and "no pathogen" groups (4.8 and 5.6 days, ...19883402153
activity of cefotaxime and amikacin against 14,272 gram-negative bacteria from clinical samples in the period 1980 to 1985.the sensitivity to cefotaxime and amikacin of 14,272 gram-negative bacilli (enterobacteriaceae and non-fermenting gram-negative bacilli) isolated from clinical samples was studied during the period 1980 to 1985. the minimum inhibitory concentration (mic) was determined by means of diffusion in agar. strains were considered resistant to cefotaxime and amikacin if the mic values were greater than 16 mg/l and greater than 8 mg/l, respectively. the mic90 reached the critical value for cefotaxime in ...19883396470
the phylogenetic origin of the bifunctional tyrosine-pathway protein in the enteric lineage of bacteria.because bifunctional enzymes are distinctive and highly conserved products of relatively infrequent gene-fusion events, they are particularly useful markers to identify clusters of organisms at different hierarchical levels of a phylogenetic tree. within the subdivision of gram-negative bacteria known as superfamily b, there are two distinctive types of tyrosine-pathway dehydrogenases: (1) a broad-specificity dehydrogenase (recently termed cyclohexadienyl dehydrogenase [cdh]) that can utilize ei ...19883386529
kell blood group activity of gram-negative bacteria.to understand better the relationships between blood-group antigens and bacterial constituents, examples of 23 gram-negative bacteria (representing the 10 genera citrobacter, edwardsiella, enterobacter, escherichia, klebsiella, proteus, pseudomonas, salmonella, serratia, and shigella) were tested for the presence of kl-like antigens by hemagglutination-inhibition (hai) assays against both igg and igm anti-kl. saline-suspended whole organisms, cell-free culture media, and disrupted organisms were ...19883368935
enrichment for plesiomonas shigelloides from stools.bile peptone broth and alkaline peptone water (ph 8.5) were examined as enrichment media for the isolation of plesiomonas shigelloides from stools, with salmonella-shigella agar as the isolation medium. after 423 parallel examinations in two different experiments, bile peptone broth enrichment for 24 h was observed to be six times more effective (p less than 0.01) than direct plating alone on salmonella-shigella agar. bile peptone broth was found to be twice as effective as alkaline peptone wate ...19883366874
susceptibilities of hong kong isolates of multiply resistant shigella spp. to 25 antimicrobial agents, including ampicillin plus sulbactam and new 4-quinolones.seventy-two percent of 129 shigella isolates from two hong kong hospitals were shigella flexneri. twenty-six percent were s. sonnei, and there was only one isolate each of s. dysenteriae and s. boydii. ninety-six percent of the isolates were resistant to two or more antibiotics, and up to 11 resistances were seen in a single isolate. fifty-seven percent or more of these isolates were resistant to ampicillin, streptomycin, tetracycline, chloramphenicol, and sulfamethoxazole; and up to twenty-thre ...19883348608
nutritional requirements of shigellae for growth in a minimal medium.most (about 81%) of the clinical isolates of shigellae that were tested failed to grow in a minimal medium. of the auxotrophic isolates belonging to the four shigella species, 98% grew in a minimal medium containing methionine, nicotinic acid, and tryptophan. the combination of methionine and tryptophan appears to be an obligatory requirement for shigella dysenteriae serotype 1 strains, while the combination of nicotinic acid and tryptophan appears to be obligatory for serotype 2. requirements w ...19883346071
invasiveness of enterobacteria related to the presence of high molecular weight plasmids.a study of 41 strains of shigella, escherichia coli, and salmonella was performed. the presence of high-molecular-weight plasmids, invasiveness in hep-2 cells and the ability to produce a positive serény test were tested. five of the seven strains of salmonella typhimurium harboured a 62 md plasmid and invaded hep-2 cells. two strains of s. typhimurium and a s. paratyphi-b strain lacked plasmids but were still invasive. among the 27 strains of shigella and enteroinvasive e. coli (eiec) 25 strain ...19883345245
proctitis and fatal septicemia caused by plesiomonas shigelloides in a bisexual man.a case of proctitis and fatal septicemia caused by plesiomonas shigelloides in a 42-year-old bisexual male is reported. the medical history of the patient was significant for an aortic valve replacement 3 years before but was otherwise unremarkable. a serum specimen obtained at autopsy was negative for antibody to human immunodeficiency virus by western blot (immunoblot) analysis. p. shigelloides isolated from blood was susceptible to all antibiotics tested, agglutinated in shigella group d anti ...19883343334
pathogenic micro-organisms and helminths in sewage products, arabian gulf, country of bahrain.fecal and sludge samples from the arabian gulf country of bahrain contained poliomyelitis and coxackie viruses, coliform bacteria, escherichia coli, salmonella spp., shigella sonni, fecal streptococci, balantidium coli, ascaris lumbricoides and hymenolepis nana eggs, and strongyloides stercoralis. sludge produced in the central sewage treatment plant is used for agricultural purposes and poses a threat to public health. recommendations to reduce the potential health hazards are made.19883341504
enteropathogens associated with acute infantile diarrhoea in cape town.the results of a year-long controlled study on the causation of acute infectious diarrhoea in infants are reported. campylobacter fetus subspecies jejuni, rotavirus and certain enteropathogenic escherichia coli (epec) types were identified as the most common enteropathogens. shigella, salmonella group b and yersinia enterocolitica were significant but less common pathogens. campylobacter, rotavirus, the significant epec types and shigella infections were more frequent in the warm summer and autu ...19883340928
aeromonas-associated gastroenteritis in children.during a 20-month period 55 strains of aeromonas species were isolated from 53 children with diarrhea. the isolation rate of 2.5% for aeromonas compared with the rates of 4.5% for shigella, 3.3% for salmonella, 2.7% for campylobacter and 0.05% for yersinia. in 45 children aeromonas was the sole bacterial enteropathogen identified. aeromonas was also isolated from 2 (0.5%) of 380 asymptomatic children. despite its known lack of identifiable virulence properties, aeromonas caviae was the most prev ...19883340460
susceptibility of enteric pathogens to amoxicillin and clavulanate-potentiated amoxicillin. 19873334666
[virulence plasmids of pathogenic enterobacteria]. 19873334270
genetics of virulence in shigella. 19863334157
shiga toxin from shigella dysenteriae 1 inhibits protein synthesis in reticulocyte lysates by inactivation of aminoacyl-trna binding.inhibition of the peptide elongation cycle of eukaryotic protein synthesis by shiga toxin from shigella dysenteriae 1 was examined in toxin-treated reticulocyte lysate mixtures. peptidyl transferase activity of toxin-treated ribosomes was measured by following the decrease in peptidyl-trna concentrations when puromycin was added after incubation with toxin. concentrations of [3h]leucine-labeled peptidyl-trna were measured by extraction with cetyltrimethylammonium bromide. the data suggest that s ...19863334156
some biological properties of outer membrane proteins of shigella.outer membrane proteins (omp) derived from two antigenically different representatives of shigella (sh. flexneri 3a and sh. sonnei phase i) were tested for the toxicity, pyrogenicity, ability to induce shwartzman reaction as well as for their influence on the leukocyte system. ld50 dose determined on mice was 28 mg/kg for omp of sh. flexneri and 23 mg/kg for omp of sh. sonnei. both preparations injected intravenously to rabbits caused moderate increase of body temperature, expressed by the value ...19873334007
outbreak of dysentery due to shigella flexneri type 2a. 19873333777
clinical features, including haemolytic-uraemic syndrome, in shigella dysenteriae type 1 infection in children of rangoon. 19873333769
a retrospective analysis of the occurrence of shigella flexneri serotypes in some african countries. 19873333153
increase in multiresistance of shigella dysenteriae type 1 strain in rwanda. 19873333152
[a comparison of manual methods and enterotube ii (roche) for the identification of gram-negative enteric bacteria isolated from feces].the enterotube ii and classic manual methods were used in parallel to identify 100 members of the enteric bacteria isolated from feces culture. among these isolated bacteria shigella, salmonella and e. coli were found 34%, 25%, 22% respectively. the enterotube ii and classic manual methods gave the same results for identification of salmonella and shigella. the enterotube ii correctly identified 98% of all gram negative enteric bacteria. other advantages and disadvantages of these methods are di ...19873332703
on a survey of plasmid dna in the clinically isolated shigella strains in shanghai area and comparison with those from south east asia. 19873331655
factors of activation and stabilization of dna-methylases from shigella sonnei 47 and mycobacterium smegmatis butyricum cells.a comparative study of factors of activation and stabilization of individual dna-methylases from two bacterial strains--shigella sonnei 47 and mycobacterium smegmatis butyricum--isolated by isoelectrofocusing in a ph gradient has been carried out. storage of enzymes at +4 degrees c (ph 7.5) is accompanied by periodic changes in the methylating activity. no such changes are observed when the enzymes are stored at pi of the protein. in this case the methylases with alkaline or close to neutral val ...19873331085
xylose lysine desoxycholate agar for recovering shigella dysenteriae 1 and shigella flexneri from faeces. 19873330107
[research on enteropathogenic bacteria from children with acute diarrhea, in belo horizonte, mg].enteropathogenic bacteria were searched in feces of 80 children with acute diarrhea from the lower social economical level, all of them younger than twenty seven months old, and without any antimicrobial therapy, in belo horizonte, mg, brazil, from june 1981 to august 1982. the following enteropathogenic bacteria were isolated from 46 children: shigella in 12 children (15.00%), enterotoxigenic escherichia coli in 12 children (15.00%), classic enteropathogenic escherichia coli in 9 children (11.2 ...19873329905
[biochemical aspects of an endotoxin model for endogenous uveitis]. 19873329451
[construction of a typhoid-dysentery bivalent strain with typhoid ty21a and dysentery t32-istrati strains]. 19873329424
[genetic determinants of antibiotic resistance in enterobacteria].antibiotic resistance of enterobacterial strains from population isolated in krasnodar region is rather often controlled by the "plasmid" genes. the conclusion is based on using the colony hybridization with [32p]-dna fragments of plasmids, carrying the genetic determinants of antibiotic resistance, as a method for antibiotic resistance, genes screening. kanamycin resistance in the majority of strains is coded by aph (3') ii gene, streptomycin resistance by aph (3") gene, chloramphenicol resista ...19873328826
[campylobacter jejuni in patients with diarrhea].in this study the stools 125 patients with diarrhea applied to research hospital of medical school of atatürk university and the stools of 69 healthy persons were investigated for gastroenteritis agents c. jejuni as well as salmonella and shigella. of the zooparasiter agents protozoon and helminths were also investigated. we determined the mentioned agents in 28 of 125 patients (% 22.40). c. jejuni was isolated the most frequent cause of diarrhea with 8.80 percent. c. jejuni was isolated in high ...19873328817
[antipuretic, antimicrobic action of gegen qinlian tang]. 19873328655
[heterogeneity of shigella flexneri during selective synchronization].the study of a number of main growth characteristics of s. flexneri (s) cells during the first and second cycles of their cultivation in a solid culture medium in peshkov's chamber from seed material, nonsynchronized and synchronized by c. e. helmstetter's method, has revealed that this method does not permit obtaining completely synchronized cultures. four physiological cell clusters, differing in the time of generation, growth rate, size at the beginning and the end of the cycle and the time o ...19873328453
the aetiology of diarrhoea in a farming community in zimbabwe.a survey of enteric pathogens in 143 children with diarrhoea, 116 age-matched controls and 524 asymptomatic people of all ages in a commercial farming area was carried out over a period of one year. giardia lamblia was detected in 34% of the cases, 29% of the controls and 23% of asymptomatic people of all ages. various bacterial pathogens (campylobacter, shigella, salmonella and enteropathogenic escherichia coli) were isolated from 23% of the cases and 15% of the controls. enteropathogenic e. co ...19873328340
differential clinical features and stool findings in shigellosis and amoebic dysentery.to obtain information that could assist the clinician to differentiate between shigellosis and amoebic dysentery, we compared clinical features and stool findings in 58 adult male patients in bangladesh. mean values indicated that patients with invasive amoebiasis were older and had a longer prehospital illness, a lower body weight, less frequent fever, a lower haematocrit and a higher white blood cell count than patients with shigellosis. the mean number of faecal leucocytes per mm3 was signifi ...19873328339
multiple drug-resistance in shigella flexneri isolated from a patient with human immunodeficiency virus.persistent shigellosis, due to shigella flexneri resistant to multiple antibiotics, developed in a 40-yr-old homosexual man with human immunodeficiency virus infection. the shigella strain demonstrated resistance to ampicillin, tetracycline, and trimethoprim-sulfamethoxazole. although shigella flexneri isolates resistant to trimethoprim-sulfamethoxazole are uncommon in the united states, laboratories should monitor resistance patterns through routine in vitro susceptibility testing.19873326711
effect of outer membrane proteins of shigella on delayed hypersensitivity in mice to sheep red blood cells.small doses of outer membrane proteins (omp) of shigella flexneri injected intraperitoneally into mice 1 to 3 days before or 3 days after sensitization of animals with sheep erythrocytes were found to increase delayed hypersensitivity as measured by the footpad reaction. in contrast, administration of higher doses of omp resulted in suppression of hypersensitivity response. cell transfer experiments showed that the spleen cells from sensitized and omp treated mice transferred stimulating and sup ...19873326545
igg response of dysenteric patients to antigens coded by the virulence plasmid of enteroinvasive pathogens.igg antibody response to antigens coded by the virulence plasmid of enteroinvasive pathogens was studied in dysenteric patients by elisa. a plasmidless escherichia coli k-12 strain and its transconjugant harbouring the 140 megadalton plasmid of a virulent e. coli 0124 strain were used as antigens. sera of 12 dysenteric patients taken on the first, third an twelfth week after shigella sonnei infection were examined. for comparison sera of 23 nondysenteric persons were also applied. titres of the ...19873326389
[reactive arthritis in enteral infections].certain gastrointestinal tract infections can trigger reactive arthritis. the best known triggering agents are yersinia, salmonella, shigella, campylobacter jejuni, and possibly clostridium difficile. the clinical findings show a subacute onset of mono- or oligoarthritis, less often of asymmetric polyarthritis with predominance of the lower limbs. the clinical picture is somewhat modified by the genetic constitution (hla type) of the patient. hla-b27-positive patients appear to have more severe ...19873326361
[the role of 4-quinolones and antibiotics in the elimination of virulence plasmids of enterobacteriaceae].twenty-six antibiotics belonging to thirteen different chemical families had been tested on seven enterobacteriaceae harbouring a virulence plasmid (shigella sonnei, s. flexneri, s. dysenteriae, escherichia coli (two plasmids). yersinia and salmonella dublin). novobiocin and rifampicin were found to be most efficient eliminating three plasmids of which two come from shigella. clindamycin, cotrimoxazole, nifurzide, ciprofloxacin, and tilbroquinol were also efficient, but at lower rate. four virul ...19873325901
[economic loss from dysentery combined with ascariasis and trichocephaliasis]. 19873325790
microbiological perspectives of co-trimoxazole.trimethoprim-sulfamethoxazole in vitro activity was compared with ampicillin, tetracycline, sulfonamide and trimethoprim against isolates of 24 gram-negative and 11 gram-positive species. the incidence of more than 10% of strains with minimal inhibitory concentrations above 32 mg/l was restricted to escherichia coli, shigella spp., klebsiella pneumoniae, providencia rettgeri, morganella morganii, methicillin-resistant staphylococcus aureus and staphylococcus epidermidis. occasionally, streptococ ...19873325434
[action of the lactoperoxidase system on salmonellae and shigellae].in vitro experiments have demonstrated that the lactoperoxidase system produces a bactericidal effect on salmonellae and shigellae. the physiological concentrations of the components of this system, making it possible to obtain a pronounced bactericidal effect, have been established. lactobacilli have been shown to potentiate the effect of the lactoperoxidase system. the possibility of realizing the bactericidal properties of the lactoperoxidase system with respect to salmonellae and shigellae i ...19873324588
evaluation of rapid sys system as screen for yersinia enterocolitica in the united states.clinical isolates (n = 150) from stool specimens were selected for evaluation of the rapid sys system (analytab products, plainview, n.y.) as a screening test for shigella spp., yersinia enterocolitica, and salmonella spp. the gram-negative identification card (vitek systems, inc., hazelwood, mo.) was used for identification. although acceptable performance of the rapid sys system was described, the interpretative criteria provided by the vendor for previous studies led to inappropriate screenin ...19873323232
clinical and field trials to assess the efficacy of vaccines against bacterial enteric infections. 19863322343
assessment of antibody screening in selection of subjects for field trials of shigella vaccines. 19863322338
synthetic peptides of shiga toxin b-subunit induce antibodies which neutralize the biological activities of the toxin. 19863322336
plasmid-mediated intracellular multiplication of shigella flexneri. 19863322175
invasion of epithelial cells by shigellae. 19863322174
plasmid-mediated contact haemolytic activity in shigella species: correlation with penetration into hela cells.the main feature of virulent strains of shigella is their ability to invade eucaryotic cells. this phenotype is both plasmid-mediated and temperature-regulated. in the present report, we demonstrate a plasmid-mediated and temperature-regulated haemolytic activity in s. flexneri, s. dysenteriae and s. sonnei. detection of this haemolytic activity requires centrifugation of suspensions containing bacteria and erythrocytes, followed by incubation of the pellets at 37 degrees c. no soluble intra- or ...19863322171
enhancement of the secretory iga response to enteropathogens. 19873321943
inhibition of enteropathogenic escherichia coli (strain rdec-1) adherence to rabbit intestinal brush borders by milk immune secretory immunoglobulin a. 19873321942
pathogenesis of shigella infections. 19873321018
frequency of escherichia coli o157:h7 isolation from stool specimens.during a 6-month period, 7252 faeces specimens were examined for escherichia coli serotype o157:h7. forty-nine specimens (0.7%) from 19 patients yielded this organism. escherichia coli o157:h7 was the third most frequently isolated bacterial pathogen, following campylobacter jejuni and (or) salmonella sp. although regional variation between laboratories determined whether campylobacter jejuni or salmonella was the primary bacterial pathogen isolated, e. coli o157:h7 was consistently isolated mor ...19873319115
elimination of a virulence plasmid from shigella sonnei and escherichia coli by antibiotics.plasmid pwr105 is a non-self-conjugative plasmid conferring enteroinvasive properties on shigella sonnei. loss of this plasmid is accompanied by loss of the invasive phenotype as well as of the form i antigen expression. thirteen antibiotics belonging to different chemical families were used at subinhibitory concentrations to eliminate pwr105 from s. sonnei and escherichia coli k12. rifampicin, novobiocin, chloramphenicol, cotrimoxazole and erythromycin eliminated the plasmid from both strains. ...19863318870
shigellosis at a caribbean resort. hamburger and north american origin as risk factors.during a three-week period in 1984, 339 tourists and expatriate employees with diarrhea visited the infirmary at a caribbean resort club. epidemiologic studies suggested that over 60% of the 1,893 tourists at the resort club during that time may have been ill. shigella flexneri 4a was isolated from seven of 18 stool specimens. a few cases of diarrheal illness occurred at the resort club before the onset of the outbreak, which was temporally related to illness in a butcher. north american residen ...19873318410
[biological properties of opportunistic microorganisms isolated from patients with acute intestinal diseases].the comparative study of the incidence of the pathogenicity markers (dnaase, rnaase, phosphatase and hemolytic activity) in shigellae and salmonellae, acknowledged as the causative agents of intestinal infections, and in opportunistic bacteria isolated from the feces of patients with acute intestinal diseases and healthy persons has been made. the study has revealed that dnaase and rnaase activity occurs most frequently in shigella flexneri, in salmonellae and in opportunistic enterobacteria iso ...19873318225
appendicitis associated with shigella sonnei dysentery. 19873316626
shigellemia and hemolytic-uremic syndrome: a case report. 19873316466
a 2d-1h-n.m.r. study of some shigella flexneri o-polysaccharides.a 1h-n.m.r. study of the o-polysaccharides from different types of shigella flexneri has been performed. with the aid of 2d-n.m.r. techniques, namely, j-resolved, spin-spin correlation, and noesy experiments, most of the structural features of these polysaccharides could be deduced. sequences could generally be obtained from the noesy experiments. when using a prolonged mixing time in these experiments, cross-peaks due to spin diffusion from one anomeric proton to the anomeric proton of an adjac ...19873315202
shigella bacteremia in adults. a report of five cases and review of the literature.shigella bacteremia is rare, occurring mainly in children. we describe five adult patients with shigella bacteremia and review data on 22 cases reported in the english-language medical literature. eighteen (67%) of 27 patients had either an underlying condition or were aged older than 65 years. most patients had clinical signs of acute febrile gastroenteritis. however, in six patients, the organism was not isolated from stool. species isolated from blood included shigella flexneri in 11 patients ...19873314766
bactericidal activities of selected organic n-halamines.the bactericidal efficacies of three organic n,n'-dihalamine disinfectants in the class of compounds termed imidazolidinones were determined for combinations of ph, temperature, and water quality treatments by using staphylococcus aureus and shigella boydii as test organisms. the compound 1,3-dibromo-4,4,5,5-tetramethyl-2-imidazolidinone was found to be the most rapidly acting bactericide, especially under halogen-demand-free conditions. the mixed n,n'-dihalamine 1-bromo-3-chloro-4,4,5,5-tetrame ...19873314705
[the mouse paw edema test for titrating shigella toxin and antitoxin and for determining the safety of the anatoxin].the method for the titration of shigella dysenteriae i neurotoxin, toxoid prepared from this toxin and different antitoxic preparations in the mouse paw edema test has been developed. the quantitative determination of antitoxin is based on the neutralization test. the conditions of titration (the dilutions of the neurotoxin and the neutralization doses of the antitoxin), as well as criteria for the evaluation of the positive or negative results, have been established.19873314277
transmissible drug resistance among shigellae and other enterobacteriaceae isolated from diarrhoeic human beings in ibadan, nigeria.antibiotic resistance patterns and the distribution of r-factors among shigella, salmonella and escherichia coli isolated from diarrhoeic human beings were studied. nine multiple antibiotic resistance patterns among which pns tck was the most common were observed. all the shigella, salmonella and eighteen escherichia coli isolates transferred part of their r determinants respectively to e. coli k12. it is thought that enforcement of the law regulating the sales and judicious use of antibiotic in ...19873314255
technological investigations with attenuated strains of shigella for production of live vaccines. 1. cultivation in a fermenter of an attenuated strain s. flexneri 2a with two markers.a method was developed for cultivation of the strain shigella flexneri 2a 77 with two attenuated markers. it ensures the preservation of the initial properties of the strain. the regimen for control of dissolved oxygen level was optimized. it was established that yeast extract is a necessary component of the nutritive medium used. the role of the phase and quantity of the inoculum for growth of the bacterial population has been clarified. fresh fermenter culture, applied in rats, shows a high im ...19873314220
[prospects for using thymalin in treating bacterial intestinal infections]. 19873314133
hemolytic uremic syndrome associated with shigellosis: report of two cases.two patients with hemolytic uremic syndrome admitted to udornthani hospital, thailand, were reported. the preceding illness was shigellosis. the stool culture was positive for shigella dysenteriae in one patient and shigella flexneri in another. the management was successful with peritoneal dialysis in one and symptomatic treatment in another. both patients had complete recovery.19873313740
in vitro activity of ciprofloxacin compared to trimethoprim-sulfamethoxazole against campylobacter spp., shigella spp. and enterotoxigenic escherichia coli causing travellers' diarrhea in egypt.the in vitro activity of ciprofloxacin against bacterial enteropathogens isolated from cases of travellers' diarrhea in egypt was compared to trimethoprim (tmp) and trimethoprim-sulfamethoxazole (sxt). no resistance to ciprofloxacin was noted for any of the campylobacter jejuni/coli, shigella spp., and enterotoxigenic escherichia coli strains examined. however, resistance to tmp and sxt was noted among these same strains. because of its broad spectrum and lack of resistance, ciprofloxacin is pot ...19873313681
[a case from practice (93). patient: mrs. c. l., born 1933, a swiss female living in tunisia]. 19873313624
[bacillary dysentery 1985]. 19873313536
trimethoprim-resistant shigella and enterotoxigenic escherichia coli strains in children in thailand.the percentage of shigella and enterotoxigenic escherichia coli (etec) strains resistant to trimethoprim (tmp)-sulfamethoxazole isolated from children with diarrhea at the outpatient department of the children's hospital in bangkok increased from 3 and 0%, respectively, in 1982 to 29% and 25% in 1986. one hundred thirty-nine shigella and 22 etec strains resistant to greater than 1024 micrograms/ml of trimethoprim (tmpr) isolated from children with diarrhea in bangkok in 1984 and 1985 were analyz ...19873313233
interferon-induced protein synthesis inhibits endocytosis of bacteria in epithelial cells.previous studies have shown that human leukocyte interferon (ifn), ifn-gamma, and recombinant (r) ifn-alpha-a protect cultured human cells against the invasiveness of salmonellae, shigellae, and enteroinvasive escherichia coli. to differentiate between effect on endocytosis and adhesiveness, hep-2 cells were pretreated with cytochalasin b to block endocytosis, and thereafter treated with ifn. although a significant reduction of adhesion of bacteria to hep-2 cells was observed, this reduction was ...19873312425
expression of campylobacter jejuni invasiveness in cell cultures coinfected with other bacteria.enteroinvasive salmonella, shigella, and escherichia coli strains were found to exert an effect which rendered campylobacter jejuni capable of intracellular localization in epithelial cells in vitro. when monolayers of hep-2 or a-549 cells were challenged with pure cultures of c. jejuni or campylobacter coli, none of the eight strains tested invaded the cells. in contrast, four of these strains were able to localize intracellularly when the cells were challenged with a mixture of campylobacters ...19873312015
introduction of shigella flexneri 2a type and group antigen genes into oral typhoid vaccine strain salmonella typhi ty21a.for protection against dysentery caused by shigella flexneri 2a, an in vivo-constructed recombinant plasmid with genes specifying the s. flexneri type and group antigens located near the pro (min 6) and his (min 44) chromosomal markers, respectively, was made and transferred to the gale salmonella typhi strain ty21a. strain ty21a carrying this recombinant plasmid was shown by immunological and biochemical analyses to express the s. flexneri 2a type and group antigens. mice immunized with this va ...19873312013
entry of shigella flexneri into hela cells: evidence for directed phagocytosis involving actin polymerization and myosin accumulation.the enteroinvasive bacterium shigella flexneri expresses a plasmid-mediated capacity to penetrate into nonphagocytic cells. by using 7-nitrobenz-2-oxa-1,3-diazole-phallacidin (nbd-phallacidin), a fluorescent dye which specifically stains microfilaments, we observed condensations of filamentous actin underneath the plasma membrane of hela cells which interacted with the invasive isolate m90t. as demonstrated by indirect immunofluorescence with the antimyosin monoclonal antibody cc-212, myosin acc ...19873312007
atrio-ventricular block associated with shigella flexneri infection. 19873311739
differentiation of shigella by esterase electrophoretic polymorphism.the electrophoretic mobilities of four esterases (a, b, c, and i) of 182 strains of shigella dysenteriae, s. flexneri, s. boydii and s. sonnei were compared to those of 636 strains of escherichia coli from various origins, including the alkalescens dispar group and enteroinvasive strains. discriminant analysis of the distribution of esterases among the strains revealed that shigella could be distinguished from e. coli by differences in the distribution of allozymes of esterases c and i. principa ...19873309172
iron regulation of shiga-like toxin expression in escherichia coli is mediated by the fur locus.shiga-like toxin is an iron-regulated cytotoxin quite similar to shiga toxin from shigella dysenteriae 1. the structural genes for shiga-like toxin in escherichia coli (slta and sltb) appear to be transcribed as an operon from a promoter upstream of slta. we used a gene fusion between the promoter and proximal portion of slta with the gene for bacterial alkaline phosphatase to assess the regulation of toxin expression. growth in low-iron conditions resulted in a 13- to 16-fold increase in alkali ...19873308853
[current antimicrobial resistance of salmonella typhi, salmonella enteritidis and shigella sp]. 19873307812
[molecular-genetic structure and incompatibility of the nonconjugative enterobacterial r plasmids pkmr281 and pkmr285].physical maps of enterobacteriaceae nonconjugative plasmids pkmr281 (sm, su, molecular weight of 6 kb) and pkmr285 (sm, su tc, molecular weight of 9 kb) were constructed for endonucleases ecori, psti, ecorv, smai, bglii, sali and pvuii. the genes controlling production of aminoglycoside-3''-phosphotransferase and dihydropteroate synthetase of type ii and the genetic tetracycline resistance determinant of class a were localized on the plasmids. heteroduplex analysis of plasmids pkmr281 and pkmr28 ...19873307606
elisa detectable virulence marker antigen of enteroinvasive escherichia coli is coded by a 140 megadalton plasmid.the elisa reactive virulence marker antigen, characteristic of enteroinvasive escherichia coli and virulent shigella, expressed in an e. coli k-12 recipient after the 140 md plasmid of an enteroinvasive e. coli strain had been introduced into it. similarly to the epithelial cell penetration and congo red binding capacities--known to be coded by the above plasmid--the virulence marker antigen expressed also only at 37 degrees c but not at 30 degrees c. these data suggest the plasmid coded nature ...19863307275
Displaying items 10901 - 11000 of 13650