amikacin therapy. use against infections caused by gentamicin- and tobramycin-resistant organisms.amikacin sulfate was used in 24 treatment courses for 25 serious infections caused by aerobic or facultative anaerobic gram-negative organisms resistant to numerous drugs. sites of infection included urinary tract (11 cases), pleuropulmonary (6 cases), primary bacteremia (5 cases), and miscellaneous (3 cases). serratia marcescens and pseudomonas sp accounted for 73% of the isolates. the mean minimal inhibitory concentration (mic) of these organisms to amikacin was 3.6 microgram/ml; to gentamicin ...1977328950
[prodigiosin as a possible inhibitor of serratia marcescens nuclease].preparations of prodigiosin inhibited the activity of nuclese of serratia marcescens. the preparations were fractionated on an alumina column. the activity of nuclease was inhibited by both fractions containing pyrryldipyrrylmethene compounds and fractions in which these compounds were not found by spectrophotometry. the inhibitor was isolated also from the cells of a pigmentless strain. therefore, the inhibition is exhibited by compounds that are extracted from the cells with acetone and petrol ...1977329065
[relationship between adsorption of microorganisms and the stage of their development].bond strength during adsorption of microorganisms depends on their growth stages, as was found by light optical and scanning electron microscopy. the strenth of adsorption often decreases as a culture growth on the surface of an adsorbent. adsorption of microorganisms is an important ecological process. under favourable conditions, microorganisms grow at a high rate when they are firmly bound to a solid surface. after abundant growth which deteriorates their microenvironment, microorganisms deso ...1977329067
[permeability of dextran-bound nuclease through the vascular barrier and tumor cell membrane].under study was the permeability of nuclease ser marcescens, bound covalently by the method of diazocombinations with m-amino benzyloxymethyl-dextran of the molecular weight 20 000, 40 000, 60 000 via the vascular barrier and the membrane of tumor cells. it is shown that dextran-bound nuclease as well as the native enzyme would penetrate into the intacts cells of ehrlich ascites carcinoma. the rate of the modified nuclease penetration is dependent on dextran molecular weight. along with the nati ...1977329582
[morphological changes in experimental infection in guinea pigs under the influence of prodigiozan and s-methylmethionine].the effect of prodigiozan and s-methylmethionine on the level of histomorphological changes in the organs and tissues of guinea pigs with experimental typhoid fever, dysentery and staphylococcal infections, as well as the effect of prednizolone with respect to dysentery and staphylococcal infections were studied. in the control animals the highest histomorphological changes were observed on the 4th day after the infection in the liver, kidneys and spleen. in the animals with staphylococcal infec ...1977329754
lipids of antibiotic-resistant and -susceptible members of the enterobacteriaceae.lipids of antibiotic-resistant and related -susceptible strains of the enterobacteriaceae were extracted with chloroform-methanol and characterized by thin-layer chromatography, densitometry, and fatty acid analysis using gas chromatography. quantitative differences which correlated with antibiotic resistance existed among the phospholipids and fatty acids. a relatively higher concentration of a ninhydrin-positive phospholipid concentration with a lower amount of phosphatidylethanolamine was obs ...1977329961
the use of nitroaromatic compounds as hypoxic cell radiosensitizers. 1977330116
teepol--an effective detergent to release l-asparaginase from serratia marcescens. 1977330391
immunocycte stimulation in vitro by nontoxic bacterial lipopolysaccharide derivatives.intact lipopolysaccharides (lps), considered nonspecific enhancers of b cell responses, as well as nontoxic derivatives from serratia marcescens lps, were studied with regard to their ability to stimulate in vitro immune responses to a t-dependent antigen, sheep erythrocytes. intact lps, at a dose of 10 to 50 microgram, consistently enhanced the in vitro anti-srbc immune response by normal splenocytes. the lps also increased the background pfc response to srbc in nonimmunized cultures. a chemica ...1977330757
[nature of the true inducer of extracellular protease synthesis in serratia marcescens]. 1977331028
the bottom of the antibiotic box. 1977331047
serratia: opportunistic pathogen of increasing clinical importance.serratia marcescens can become a formidable nosocomial (hospital acquired) pathogen, and is reported increasingly in the world literature. however, it is only a recently recognized problem in australia. serratia can carry an antibiotic-resistance plasmid, and, after entry of the organism into very sick patients, it may be hard or impossible to eliminate. initial experience of serratia in 34 consecutive cases isolated in a three-months period is presented. rapid increase in the number of serratia ...1977331049
[characteristics of the functional activity of human blood neutrophils using the nitroblue tetrazolium reduction test].a study was made of the test of restoration of the nitroblue tetrazolium (nbt) by the intact blood neutrophils and the neutrophils stimulated with various endotoxin doses of gram-negative bacteria in 70 healthy persons. a high sensitivity of the test with the nbt for the quantitative assessment of the neutrophil response under conditions stimulating the phagocytic activity was demonstrated. a functional nonhomogeneity of the neutrophil population, the most distinct with the minimal stimulating a ...1977331772
[an epidemic caused by serratia marcescens in an intensive-care unit for premature and other newborns (author's transl)].an epidemic caused by serratia marcescens occurred in intensive care unit of the children's clinic in essen, with three deaths. although there was good sensitivity of the strain to gentamicin in vitro, there was no noticeable clinical improvement when it was administered. but cotrimoxazole, given systemically and locally, and colistin locally cured the disease.1977332480
assessment of typhoid vaccines by using the intraperitoneal route of challenge.present laboratory tests for human typhoid vaccines use an intraperitoneal route of challenge given 7 days after injection of increasing doses of standard and test vaccines by the same route. in studies reported here, groups of b6d2 mice were vaccinated intraperitoneally with 2 x 10(8) acetone-killed salmonella typhi ty2, with the vi antigen-free variant o-901, or with yersinia enterocolitica and serratia marcescens suspensions. other groups of mice received 200 mug of purified s. typhi or s. ma ...1977332627
[correlation between the synthesis of extracellular proteases and the synthesis of the red pigment prodigiosin in serratia marcescens].a correlation has been established between synthesis of exocellular protease and synthesis of a red pigment prodigiosine by serratia marcescens. chloramphenicol, an inhibitor of protein synthesis, inhibits also synthesis of the pigment. leucine, an inductor of synthesis of the exocellular protease by serratia marcescens vi, induces also synthesis of the pigment. a mixture of 18 natural amino acids, asparagine and ammonium ions represses both synthesis of the enzyme and the pigment.1977333235
structure of chromatin subunits: an endonuclease serratia marcescens study.electrophoretic properties of chromatin subunits--nucleosomes--obtained by treatment of chromatin with the serratia marcescens endonuclease have been studied. double-stranded breaks of dna between adjacent nucleosomes do not necessarily lead to their disjunction. fragmentation of the dna within the nucleosomes may proceed simultaneously with the breakdown of the dna between the nucleosomes at early stages of the endonuclease digestion. electrophoretic mobility and chromatographic properties of m ...1977333401
norleucine accumulation by a norleucine-resistant mutant of serratia marcescens.a norleucine-resistant mutant was derived from an isoleucine-valine auxotroph of a leucine accumulator of serratia marcescens. the norleucine-resistant mutant could accumulate norleucine from norvaline in the medium without the addition of methionine, which antagonized norleucine. this mutant constitutively formed homoserine-o-transsuccinylase.1977334070
evidence for incorporation of thymidine into deoxyribonucleic acid in airborne bacterial part of an effort to discover whether bacteria might propagate within airborne particles, we studied the incorporation of thymidine into the trichloroacetic acid-insoluble fraction of airborne cells of serratia marcescens to seek evidence of the possible formation of new dna. two aerosols, one of s. marcescens and another of [3h]thymidine ([3h]dt) suspended in growth medium were caused to aggregate in air just prior to directing the aerosols into rotating-drum aerosol storage chambers. the ag ...1977334075
serratia marcescens pneumonia.though rare, serratia marcescens pneumonia is being reported with increasing frequency, especially in patients in intensive care units. we report three cases of s. marcescens pneumonia that presented striking similarities for age, group, type of surgical procedure, and microbiological, hemodynamic, and respiratory patterns. all patients survived after prolonged ventilatory support.1977334114
[distribution of the dimensions of cells and intracellular structures of biosystems from measurements of the diffusion of water by the impulse gradient of the nmr spin echo method].the method of impulse gradient of nmr spin-echo was applied for studying limited water diffusion in yeast cells and embryons of wheat caryopsis. results are presented of measuring the amplitude of the echo signal depending on the intensity of the impulse gradient of the magnetic field reaching the values up to 1.7 kgs/cm. the method is suggested of determining on the basis of experimental data the distribution function parameters of the sizes of limiting cell compartments taking into account the ...1977334271
canine granulocytopathy syndrome: defective bactericidal capacity of neutrophils from a dog with recurrent infections. 1977334401
enzymatic degradation of polygalacturonic acid by yersinia and klebsiella species in relation to clinical laboratory scored by several specified plating procedures, clinical and environmental strains of yersinia enterocolitica, yersinia pseudotuberculosis, and klebsiella pneumoniae "oxytocum" showed detectable, albeit generally weak, ability to digest polygalacturonic (pectic) acid. none of these bacterial strains had the vigorous and rapid pectolytic activity on these polygalacturonic acid-containing media that is typical of soft-rot erwinia species, although some of the oxytocum strains came fairly close. ...1977334794
[soviet research on finding and studying antibiotics and other biologically active substances of natural origin]. 1977335953
selected and spontaneous variants of serratia marcescens with combined resistance against chloramphenicol, nalidixic acid, and trimethoprim. 1977336309
serratia marcescens septicaemia in newborn. 1977336528
radiosensitization of serratia marcescens by bipyridinium compounds.bipyridinium compounds (viologens) have been shown to radiosensitize hypoxic serratia marcescens cells by two components. these can be separated on the basis that only the one-electron reduced form of the compounds can penetrate the bacterium cell wall. one component is associated with sensitization at the membrane and the other with an internal site. the efficiency of sensitization at the membrane-associated site follows the order of increasing one-electron reduction potentials of the compoun ...1977336568
antibody response to serratia marcescens isolated from patients with malignant diseases.sixty-four patients with malignant diseases from whom serratia marcescens was isolated from various sources were studied regarding their antibody responses to somatic o antigens of this microorganism. antibodies were titrated by the passive hemagglutination test. an antibody response was considered present when either a fourfold or greater rise in antibody titers between two consecutive serum specimens was demonstrated, or when elevated titers (greater than or equal to 40 for serogroup o14 and g ...1977336643
the significance of serratia as an infectious organism.the hospital records of 48 patients with infections due to serratia marcescens were reviewed. isolates from these patients had been cultured during the period from august 1973 through july 1975, at which time an increase in frequency of infections due to serratia had been noted. most of these patients were elderly males with chronic debilitating diseases. all patients had received antimicrobial therapy prior to the time serratia was first isolated. the majority had had indwelling urinary cathete ...1978337544
l-histidine production by histidase-less regulatory mutants of serratia marcescens constructed by transduction.2-methylhistidine (2mh) and 1,2,4-triazole-3-alanine (tra) inhibited the growth of serratia marcescens. these inhibitory effects were counteracted by l-histidine. enzymatic studies showed that 2mh acts as a false feedback inhibitor and tra acts as both a false feedback inhibitor and a repressor. mutants resistant to each analog were isolated from a histidase-less mutant, because the wild-type strain possesses a potent histidase activity. 2mh-resistant mutants had a feedback-insensitive phosphori ...1977337893
rapid identification and quantitation of small numbers of microorganisms by a chemiluminescent immunoreaction.a method (patent pending) for rapidly identifying and quantitating small numbers of microorganisms was developed based on the specific immunoreaction of microorganisms with homologous antibodies linked by conjugation to peroxidase. the high sensitivity of the method is due to the use of a chemiluminescent reaction for the determination of the enzyme. the reaction was performed on alcar supports with low nonspecific adsorption. the very low noise achieved permitted the detection of as few as 30 t ...1977337894
serratia marcescens cellulitis. 1977337905
nocardicin a, a new monocyclic beta-lactam antibiotic v. in vivo evaluation.nocardicin a is a new monocyclic beta-lactam antibiotic which provides a potent therapeutic effect in mice experimentally infected with gram-negative bacilli. when given subcutaneously to mice, the therapeutic effect of the drug was stronger than had been anticipated from in vitro studies. nocardicin a was more potent in therapeutic effect than carbenicillin against infections due to pseudomonas aeruginosa, proteus mirabilis, pr. vulgaris, pr. rettgeri and pr. inconstans, and was similar in effe ...1977338567
quality assurance of sterilized products: verification of a model relating frequency of contaminated items and increasing radiation dose. 1977338569
purification and properties of a third form of anthranilate-5-phosphoribosylpyrophosphate phosphoribosyltransferase from the enterobacteriaceae.anthranilate-5-phosphoribosylpyrophosphate phosphoribosyltransferase was purified from the bacterium erwinia carotovora, a member of the enterobacteriaceae. the enzyme was homogeneous according to the criteria of gel electrophoresis and nh2-terminal amino acid sequence analysis. the molecular weight of the enzyme as determined on a calibrated sephadex g-200 column was 67,000 +/- 2,000. sodium dodecyl sulfate-polyacrylamide gels gave a subunit molecular weight of 40,000 +/- 1,000, suggesting that ...1978338606
[urinary tract infections due to serratia marcescens. i. antimicrobial susceptibility patterns of clinical isolates of serratia marcescens (author's transl)]. 1977338940
[serratia in urinary tract infection. ii. clinical significance (author's transl)]. 1977338941
[antitumor action of ser. marcescens nuclease covalently bound to soluble dextrans].the antitumor effect of ser. marcescens nuclease, modified by covalent binding using the method of diazocoupling with m-aminobenzyloxymethyl dextran of molecular weight 20 000, 40 000, 60 000, exceeds 3--4 times the antitumor effect of the native enzyme in relation to ehrlich ascites carcinoma in contact tumor cells exposure. in intramuscular injection of the enzyme the nuclease preparation modified by dextran of molecular weight 40 000 showed the greatest antitumor activity against ehrlich carc ...1977339537
enhancement of isoleucine hydroxamate-mediated growth inhibition and improvement of isoleucine-producing strains of serratia marcescens.growth inhibition by isoleucine hydroxamate in serratia marcescens was significantly enhanced by adding valine plus leucine and by using glycerol as the carbon source. isoleucine hydroxamate-resistant mutants were isolated under conditions in which growth inhibition was enhanced. one of the mutants, strain gihvlr2179, lacked both feedback inhibition and repression of threonine deaminase. an alpha-aminobutyric acid-resistant mutant derived from strain gihvlr2179, strain gihvlar2795, produced 12 m ...1977339830
cross-infection with serratia marcescens. 1978340004
aminoglycoside inactivating enzymes produced by r plasmids of escherichia coli, citrobacter freundii, klebsiella pneumoniae, proteus vulgaris, providencia stuartii and serratia marcescens. 1977340445
gram-negative endocarditis following cystoscopy.patients with bacteriuria are at risk for local and distant infectious complications at the time of urologic procedures. the american heart association recommends that penicillin and streptomycin be given prophylactically to patients with rheumatic or congenital heart disease without reference to the presence or absence of bacteriuria. a patient with unrecognized calcification of the mitral annulus who underwent cystoscopy for evaluation of urinary retention is reported. although bacteriuria was ...1978340713
[the effect of glucose on induced synthesis of exocellular protease of serratia marcescens].the sensitivity of induced synthesis of exocellular protease to catabolyte repression was studied in serratia marcescens growing on media containing inductors, viz. leucine and albumin. a lower sensitivity of the leucine-induced synthesis of the enzyme to glucose as compared to that induced by albumin seems to be caused by the penetration of leucine into the cell prior to the appearance in the medium of organic acids, possible inhibitors of its transport, whereas on the medium containing albumin ...1977340852
infection in the intensive care epidemic of infection associated with serratia marcescens and other gram-negative organisms resistant to aminoglycosides and other chemotherapeutic agents occurred in the intensive care unit of st vincent's hospital, melbourne, and spread to other areas of the hospital. this paper describes the problems of sepsis in the critically ill patient, outlines the occurrence of organisms in the patients concerned in this epidemic, and discusses the policies adopted to control the incidence of life-th ...1977340863
[serratia marcescens in bovine mastitis. bibliography and a case study (author's transl)].the literature concerning serratia marcescens in bovine mastitis is reviewed, and a case of acute mastitis at the department of obstetrics, the veterinary college of norway, in which serratia marcescens was isolated in pure culture, is described. serratia marcescens usually causes only moderately severe symptoms of mastitis, but relapses are common and cases tend to become chronic in nature. the bacterium produces an endotoxin, and this toxin has caused acute mastitis on experimental inoculation ...1977341073
effect of clays on the microbe adsorption.the non motile gram-positive spore-forming bacteria are much more adsorbed on the bentonite than the motile gram-negative rods. the motile bacteria adjance to clays in the phases of their rapid multiplication only. the dependence of the clay adsorption of motile microbes by the substrate and/or intermediate adsorption has been suggested.1977341596
[the influence of particulate matter and gaseous pollutants on the resistance against infectious diseases (author's transl)].groups of female nmri-mice inhaled nine weeks 12.4 or 81.8 microgram pb/m3 24 h per week, while other groups inhaled 0.3 mg no2 + 5 mg flame soot/m3 or 5 mg no2 + 0.3 mg flame soot/m3 for 45 h/week. five animals of each group were randomly selected in weekly intervals and bacterial elimination determined 5 hours after inhaling a serratia marcescens-aerosol. bacteria in lung sections were determined by means of the "sandwich-method", using an anti-serratia-serum and a fitc-loaded antirabbit-gamma ...1977341603
activity of eight aminoglycosides against isolates of serratia marcescens from four hospitals. 1978342482
chloroquine resistant plasmodium falciparum in thailand: susceptibility of anopheles. 1977342649
a study on the microbial filtration efficiency of surgical face masks--with special reference to the non-woven fabric mask.with the experimental apparatus designed and made available by nicholes, we evaluated the bacterial filtration efficienty (b.f.e.) of the non-woven fabric and cotton cloth masks. the apparatus was supplied by nicholes (u.s. military specification 36954 c mask, surgical, disposable). the study presented here was performed as a round robin test with nicholes. by using this apparatus, comparison was made as to the b.f.e. of the six different kinds of surgical face mask before and after prolonged us ...1978343940
unsustained multiplication of treponema pallidum (nichols virulent strain) in vitro in the presence of oxygen.treponema pallidum (nichols virulent strain) was incubated with or without oxygen using a modified medium supplemented with reduced glutathione and a variety of nutrients (prnf10-b). two- to fourfold increases in treponemal numbers were observed in cultures without mammalian cells within 96 h of incubation under 5 to 6% oxygen. treponemal motility and multiplication were maintained more satisfactorily in cultures that were diluted and transferred daily, using an equal volume of fresh medium. tre ...1978344209
acid precipitation of clostridium botulinum type c and d toxins from whole culture by addition of ribonucleic acid as a precipitation aid.the ratios of ribonucleic acid to protein contents of clostridium botulinum type c, d, and e cultures were lower than those of type a, b, and f cultures. addition of ribonucleic acid at 0.4 mg/ml to culture satisfactorily aided acid precipitation of type c and d toxins, but not that of type e toxin.1978344224
production and traffic of b lymphocytes in the extracortical central area of the guinea pig thymus.lymphocytes in the stroma and lymphatics of the extra-cortical central area (ecca) of the guinea pig thymus have been studied with light microscopy, quantitative microscopy, colchicin-induced mitotic arrest, ea (igg) and ea (igm) c adherence, surface immunoglobulins (ig total, igm, igg), alkaline phosphatase activity and the effect of cyclophosphamide administration. the results suggest, that ap-positive, sig-positive eac-negative lymphocytes in the ecca proliferate and maturate into ap-negative ...1978344232
the comparative synergistic activity of amikacin, gentamicin, netilmicin and azlocillin, mezlocillin, carbenicillin and ticarcillin against serratia marcescens.the synergistic activities of netilmicin, gentamicin and amikacin combined with carbenicillin, ticarcillin, azlocillin and mezlocillin were investigated against 32 serratia marcescens isolates. synergy could be demonstrated by killing curve technique, isobologram plots as susceptibility data with any of the aminoglycosides and penicillins combinations. no antagonism was shown with any of the combinations. the majority of the isolates were resistant to the aminoglycosides and penicillins. combina ...1978344298
persistence of enterobacteriaceae in female adults of the biting gnat culicoides variipennis (diptera: ceratopogonidae). 1978344884
electiveness of photorepair, influence of dark-repair on shape of dose-response curves, and high-dose decline, in uv-induced colour mutations of serratia. 1978345116
long-term adjuvant effect of bacterial endotoxin in prevention and restoration of radiation-caused immunosuppression. 1978345282
[effect of gentamycin, decamethoxin and prodigiozan on the course and outcome of experimental pyocyanic infection in white mice].the therapeutic and prophylactic effectiveness of gentamicin, decamethoxin and prodigiozan was determined on albino mice infected with two-fold lethal doses of the antibiotic-resistant strain of ps. aeruginosa. correlation between the effectiveness of the drug and the dose and time of its administration was found. pronounced prophylactic and therapeutic effect of gentamicin in combination with decamethoxin was noted. prodigiozan, a bacterial polysaccharide had no significant effect on the experi ...1978345960
[stimulating effect of prodigiozan on nonspecific reactivity in typhoid fever, dysentery and staphylococcal infection].a number of factors of nonspecific reactivity, i.e. complement, lysozyme, properdin, blood serum bactericidal activity, leucocyte phagocytal activity, phagocytal index and completed phagocytosis were studied on 160 guinea pigs with experimental typhoid fever, dysentery and staphylococcal infections. the data of the study showed that with the use of prodigiozan the non-specific reactivity of the host increased even within a short period of time. at the same time the pathological changes in the in ...1978345961
construction of a urocanic acid-producing strain of serratia marcescens by serratia marcescens, the mutation responsible for triazolealanine (tra) resistance was transferred from a tra-resistant mutant to a urocanase-less mutant by ps20-mediated transduction. the two crosses were performed using as donors two tra-resistant mutants, whose phenotypes included increased levels of histidine-biosynthetic enzymes and feedback-insensitive phosphoribosyltransferase. in one cross, tra-resistant transductants were urocanase-less mutants having only increased levels of the enz ...1978345965
increased antimetabolite sensitivity with variation of carbon source during serratia marcescens, analogs of leucine (norleucine), methionine (alpha-methylmethionine), histidine (3-amino-1,2,4-triazolealanine), tyrosine (p-aminophenylalanine), and tryptophan (7-methylindole) are conditional inhibitors of growth; inhibition occurs during the metabolism of some carbon sources but not with others. a further increase in sensitivity to growth inhibition by these analogs can be accomplished through the use of particular combinations of carbon sources present in the inoculum ...1978346563
bactericidal and opsonic activity of cirrhotic ascites and nonascitic peritoneal and oa of sera and uninfected ascitic fluid from patients with alcoholic cirrhosis were assayed against gram-negative enteric bacilli. this was compared with ba and oa in normal serum and in peritoneal fluid obtained at laparoscopy or laparotomy from noncirrhotic patients. cirrhotic sera showed significantly reduced ba and oa against one of the organisms tested, serratia marcescens. it had reduced oa but normal ba against e. coli. ascitic fluid was markedly deficient in ba and oa against all ...1978347014
[in vitro activity of cefoxitin compared with the activity of other antibiotics and trimethoprim-sulfamethoxazole (author's transl)].the new developed antibiotic cefoxitin has been tested in vitro on its effectiveness against bacteria isolated from human material. pseudomonas aeruginosa and streptococci of the serological group d were not tested, because these microorganisms are not sensitive against this antibiotic. besides cefoxitin other antibiotics (cephalothin, cephalexin, ampicillin, carbenicillin, tetracycline, gentamicin, penicillin g, oxacillin) and trimethoprim-sulfamethoxazole were also tested. as to the results ce ...1978347234
[serratia marcescens as a cause of nosocomial infection in an intensive care unit]. 1978347696
[the problem of anti-lung basal membrane immunity in the framework of allogenic lung transplant rejection].this study is concerned with the significance of autoimmune processes caused by the damage of lung tissue after lungallo- and autotransplantation. in 15 mongrel dogs a left side lung-allotranplantation was performed, 5 of them were treated with immunsuppressive therapy in the posttransplantation period. a group of 5 dogs with autotransplantation of the left lung served as a control-group. in the posttransplantation period the development of humoral antibodies responding with lung basal membrane ...1978347733
gentamicin use and pseudomonas and serratia resistance: effect of a surgical prophylaxis outbreak of prosthetic valve endocarditis due to methicillin-resistant staphylococcus epidermidis prompted a change in antimicrobial prophylaxis for open heart surgery in a general hospital from a regimen of aqueous penicillin g, methicillin, and kanamycin to a 5-day regimen of cefazolin and gentamicin. as a result, total gentamicin use in the hospital more than doubled. increased resistance of pseudomonas and serratia isolates paralleled the increased total use of gentamicin. for pseudomonas ...1978348094
amikacin therapy of gram-negative bacteremia and meningitis. treatment in diseases due to multiple resistant bacilli.the therapeutic efficacy of amikacin was evaluated in patients with serious hospital-acquired infections caused by gram-negative bacilli susceptible to amikacin, but usually resistant to kanamycin, gentamicin, and tobramycin. the infections for which amikacin was given were gram-negative bacteremia in 15 patients and gram-negative meningitis in two patients. therapy with amikacin resulted in a cure in 13 patients, improvement in 1, and failure in 3. continuous intravenous infusion of amikacin yi ...1978348135
pleiotropic consequences of mutations towards antibiotic-hypersensitivity in serratia marcescens.various mutants (oxas) were isolated from serratia marcescens sm-6 by selecting for hypersensitivity towards oxacillin. all mutants found are highly pleiotropic and able to yield spontaneous revertants which behave like the wild-type. mutant w 1421 mostly studied shows the following phenotypic properties not found in the wild-type: (1) the growth is hypersensitive to various antibiotics, detergents and dyes which differ remarkably in their chemical structure and antibacterial action-mechanism, ( ...1978348145
primary structure of protein s19 from the small ribosomal subunit of escherichia coli. 1978348496
l-arabinose-ornithine-irgasan medium for differentiating serratia species.a semisolid medium (designated serratia differentiation medium) containing l-arabinose, ornithine, and selective inhibitor was used to differentiate three clinically encountered serratia species. the inhibitor, irgasan dp-300, was incorporated to eliminate false-positive reactions from most remaining enterobacteriaceae. the suspected serratia colony was inoculated as a stab into the medium. serratia marcescens was indicated by a change in color from olive to purple following 18 h of incubation, ...1978348720
differences between cephalothin and newer parenterally absorbed cephalosporins in vitro: a justification for separate disks.the activities of cefamandole and cefoxitin in vitro were compared with that of cephalothin against staphylococci and gram-negative bacilli. cephalothin was the most active agent against staphylococci. cefamandole exhibited the greatest activity against the enterobacteriaceae, with the exceptions of serratia marcescens and indole-positive proteeae, against which cefoxitin was the most active antibiotic. the activity of newer cephalosporins that are resistant to the beta-lactamases of gram-negati ...1978349096
genetic studies of the ribosomal proteins in escherichia coli. xi. mapping of the genes for l21, l27, s15 and s21 by using hybrid bacteria and over-production of these proteins in the merodiploid strains.e. coli episomes which cover argg region were transferred to s. marcescens and ribosomal proteins (r-proteins) from these hybrid strains were analyzed by two-dimensional (2d) polyacrylamide gel electrophoresis. three e. coli r-proteins l21, l27 and s15 could be detected in the ribosomes from the hybrid strains. the relative rate of synthesis of the individual r-proteins were determined for e. coli merodiploid strains harboring these episomes. over-production of three r-proteins l21, l27 and s21 ...1978349351
[sensitivity of serratia marcescens to chemotherapy].the sensitivity of 112 s. marcescens strains, isolated under various clinico-epidemiologic conditions, was tested by the dilution in agar method against 10 different antibiotics and sulfonamides active against gram-negative bacteria. with the maximum concentrations used only gentamycin and nalidixic acid were active against a high proportion of the strains tested, i.e. 96.4% and 91.1%. kanamycin, neomycin, chloramphenicol, streptomycin and tetracyclin had an inhibitory effect against less than 2 ...1978349661
threonine production by regulatory mutants of serratia marcescens.beta-hydroxynorvaline (alpha-amino-beta-hydroxyvaleric acid)-resistant mutants of serratia marcescens deficient in both threonine dehydrogenase and threonine deaminase were isolated and characterized. one of the mutants, strain hnr21, lacked feedback inhibition of threonine-sensitive aspartokinase and homoserine dehydrogenase, was repressed for the two enzymes, and produced 11 mg of threonine per ml of medium containing a limiting amount of isoleucine. the other mutant, strain hnr59, was constit ...1978350154
[a study on the effectiveness of the elimination of bacteria by an a-type air purifier]. 1977350928
[combination of exchange transfusion and directed antibiotic therapy in the treatment of severe sepsis. a case of multi-resistant serratia marcescens sepsis]. 1978351366
[resistance of serratia marcescens to an etbylene oxide-methyl bromide mixture and the possibility of using it to control the effectiveness of disinfectant measures].the authors studied the serratia marcescens (strain no. 851) resistance to the okćbm mixture by the method of test objects in comparison with the vaccine virus (strain b-51) at a temperature of 20, 30, and 40 degrees c. resistance of the mentioned bacteria to the okćbm mixture proved to be somewhat greater than that of the vaccine virus at 20 and 30 degrees c, whereas at 40 degrees c their resistance was found to be practically identical. this permits to use serratia marcescens to control the ef ...1978352054
inhibition of pyruvate dehydrogenase multienzyme complex from escherichia coli with mono- and bifunctional arsenoxides. 1978352396
a rapid high-yield purification procedure for the cyclic adenosine 3',5'-monophosphate receptor protein from escherichia coli.a new method for the purification of the cyclic adenosine 3',5'-monophosphate receptor protein from escherichia coli has been developed. the method is rapid and simple, and a very high yield of the purified protein is obtained which is not contaminated with either dnaase or rnaase activity.1978352408
[role of normal microflora in the treatment of vibrio carrier state in rat gnotobionts].materials on the study of interactions between vibrio cholera el tor and some indigenous bacteria of human intestinal tract, which proved to be active antagonists in vitro, are presented in this work. experiments were conducted on gnotobiotic rats (fisher), using various combinations and variants of the order of the microorganism administration; there were revealed no antagonistic relations between virbrio cholerae el tor and escherichia coli m-17, lactobacillus fermenti, lactobacillus plantarum ...1978352437
antibiotic susceptibility of clinical isolates of serratia marcescens compared with sensitivity to group a (phage tail) bacteriocins. 1978352637
membrane protein segregation during release of microvesicles from human erythrocytes. 1978352723
reversal of jejunal water secretion by glucose in rats exposed to coliform enterotoxins.glucose absorption and glucose-facilitated water transport were assessed in rats exposed to semipurified preparations of the heat-labile (lt) and heat-stable (st) enterotoxins of escherichia coli, klebsiella pneumoniae, and enterobacter cloacae by in vivo jejunal perfusion of these toxins alone and with varying amounts of glucose. progressive increases in the glucose concentration of from 12 to 56 mm resulted in incremental rises in water absorption from perfusates containing each of these toxin ...1978352789
release of granulocyte elastase in lethal canine endotoxin shock.the release of granulocyte elastase and its interaction with plasma protease inhibitors was studied in dogs receiving a slow infusion of a lethal dose of escherichia coli endotoxin. during endotoxin infusion a marked decline in leucocyte counts was parallelled by a rapid increase in plasma granulocyte elastase concentrations. maximal values were reached after 3 h, when the infusion was ended. crossed immunoelectrophoresis with antiserum against granulocyte elastase did not reveal the presence of ...1978352893
some characteristics of heat-stable enterotoxin of escherichia coli. 1977352921
pathogenesis, lethality, and immunizing effect of experimental cutaneous cryptococcosis.mice were subcutaneously inoculated with small numbers of virulent cryptococcus neoformans and divided into groups. numbers of viable yeasts at the site were estimated at weekly intervals for 5 weeks on the basis of cultures of minced tissue excised from sacrificed animals. organisms multiplied at the site for at least 4 weeks and were still detectable after the 5th week, although in reduced numbers. agglutinins appeared within a week, but these antibodies were not detectable during the 2nd thro ...1978352944
role of the surface coat in in vitro attachment and phagocytosis of plasmodium berghei by peritoneal macrophages.evidence is presented to indicate that plasmodium berghei merozoites, but not trophozotites, have an antiphagocytic capsule. the capsule appears to form around the developing merozoties of the schizont in the parasitophorous vacuole. serum from animals immune to p. berghei reacts with this capsule. after reaction with immune serum, the antiphagocytic action of the capsule is lost. by the process of binding serum protein, the capsule becomes electron dense and can be readily visuallzed as the sur ...1978352960
biotyping of serratia marcescens and its use in epidemiological studies.a serratia marcescens biotyping system using eight carbon sources (benzoate, dl-carnitine, m-erythritol, 3-hydroxybenzoate, 4-hydroxybenzoate, lactose, d-quinate, and trigonelline), a tetrathionate reduction test, production of prodigiosin, and horse blood hemolysis was derived from a recent numerical taxonomic study (grimont et al., j. gen. microbiol. 98:39-66, 1977). a total of 98.6% of 2,210 isolates from various sources could be assigned to 1 of 19 biotypes. distribution and spread of 1,088 ...1978353073
[effect of low temperatures (-196 degrees) and of cryoprotectors on some bacterial species].the effect of low temperatures (-196 degrees c) and cryoprotectors (peo-400 and glycerol) on the survival, morphological and functional properties was studied with escherichia coli, serratia marcescens and staphylococcus aureus 209. when the cells were frozen for a short period of time in liquid nitrogen, the survival and the rate of protein synthesis decreased in the gram-negative bacteria but remained almost the same in staphylococcus aureus. peo-400 and glycerol manifested cryoprotecting acti ...1978353449
[treatment of a case of endocarditis caused by serratia with bacteriophages]. 1978353719
[selected chapters from clinical bacteriology. vi. enteric bacteria]. 1978353784
radiosensitization of serratia marcescens by nitropyridinium compounds.the two nitropyridinium compounds tested sensitize hypoxic serratia marcescens to irradiation up to the oxygen enhancement level by two components which can be separated as a function of compound concentration. sensitization above the initial plateau level is in order of their determined one-electron reduction potentials, ro 03-5580 (e 7 1 = -335 mv) being more efficient than ro 03-5637 (e 7 1 = -358 mv). additivity in sensitization up to a maximum enhancement level of 2.1 +/- 0.1 is found on c ...1978354674
sensitization of ultraviolet radiation damage in bacteria and mammalian cells.bacteria (serratia marcescens) and mammalian cell (chinese hamster v79-379a) were irradiated in monolayers with ultraviolet light at 254 nm or 365 nm in the presence or absence of radiosensitizing drugs. at 254 nm, killing is very efficient (d37 approximately 1 j m-2 exposure, or approximately 6 x 10(4) photons absorbed by dna per bacterium), and sensitizers have no effect. at 365 nm, cells are not killed in buffer, but are inactivated in the presence of nifurpipone or misonidazole. lethal expo ...1978354675
metronidazole (flagyl) and misonidazole (ro-07-0582): reduction by facultative anaerobes and cytotoxic action on hypoxic bacteria and mammalian cells in vivo.the toxic actions of the "nitro" radiosensitizers, metronidazole and misonidazole on the bacteria e. coli b/r and serratia marcescens have been investigated under anareobic and aerobic conditions. the rates of reduction of the drugs by suspensions of these bacteria as well as by suspensions microorganisms from the rat caecum have been measured. both drugs were reduced or were toxic only under anaerobic conditions. in all instances misonidazole was reduced more rapidly than metronidazole but metr ...1978354677
a fast kinetics study of the modes of action of some different radiosensitizers in bacteria.using a fast mixing a irradiation technique, the gas explosion method, with serratia marcescents, the decay of oxygen-dependent damage is found to consist of a fast and a slow stage, each of which is associated with a sub-component of this damage. in the present work, the interactions of these components with radiosensitizers are examined. at low concentrations o2, tan (a nitroxyl) and misonidazole all preferentially sensitize the slow-stage damage. at higher concentrations, o2 and tan sensitize ...1978354678
effect of haloperidol and d-amphetamine on cerebral tyramine and octopamine levels.the administration of d-amphetamine or haloperidol produced a marked reduction in the rat striatum concentration of p-tyramine, an effect that was not observed in the mesolimbic system. however, the administration of d-amphetamine to haloperidol-pretreated animals produced in both brain areas a marked reduction in p-tyramine levels. furthermore, this latter treatment produced a marked increase in the m-tyramine levels in both brain regions. hypothalamic p-octopamine levels were reduced by d-amph ...1978354941
threonine degradation by serratia marcescens.the wild strain of serratia marcescens rapidly degraded threonine and formed aminoacetone in a medium containing glucose and urea. extracts of this strain showed high threonine dehydrogenase and "biosynthetic" threonine deaminase activities, but no threonine aldolase activity. threonine dehydrogenase-deficient strain mu-910 was selected among mutants unable to grow on threonine as the carbon source. this strain did not form aminoacetone from threonine, but it slowly degraded threonine. strain d- ...1978355220
differentiation of serratia marcescens and serratia liquefaciens by tests for lipase and phospholipase production.the production of lipase and phospolipase by certain members of the enterobacteriaceae was examined by thin-layer chromatography of resting-cell suspensions incubated with triolein or lecithin. most strains of serratia marcescens produced both enzymes while most strains of serratia liquefaciens exhibited strong lipase but only a minor phospholipase activity. enterobacter spp. (25 strains), klebsiella pneumoniae (20 strains), escherichia coli (15 strains), citrobacter freundii (7 strains) and pro ...1978355631
cervicofacial abcesses of unknown origin. a survey of eighty-one cases.a survey of eighty-one patients with cervicofacial abscesses of unknown origin is presented. the salient clinical features and the treatment of the abscesses are described. mainly affected were children under 4 years of age, and the submandibular and submental regions were the most common sites of involvement. staphylococcus aureus was the predominant pathogenic organism, and epidemic strains were implicated in some cases. a high incidence of resistance to penicillin was noted. the nasal vestibu ...1978355964
control of acetohydroxy acid synthetase in escherichia coli 9723.a method by which three acetohydroxy acid synthetase activities are separated from extracts of escherichia coli 9723 has been developed. isoleucine specifically represses synthesis of one of the enzymes, which is not sensitive to valine inhibition, and isoleucine also simultaneously enhances the production of a second activity, which is valine inhibitable. the valine-inhibitable activity is repressed by leucine and valine, a combination of which is more effective than either alone. the third ace ...1978356876
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