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inhibition of atp synthesis associated with 6-aminonicotinamide (6-an) teratogenesis in rat embryos.pregnant rats were injected ip with 6 mg/kg 6-aminonicotinamide (6-an) at day 12 of gestation. embryos removed between 1 and 48 h later had reduced adenosine triphosphate (atp) concentrations, of about 50% of control values. all fetuses examined near term were malformed. nicotinamide (nam, 100 mg/kg) given ip 1 h after 6-an afforded protection: malformations occurred in only 15% of the survivors; and there was minimal atp reduction, 15% below control values. nam given 2 and 4 h after 6-an produc ...1975128147
suppression of allosensitization in vitro by cells from tolerant rats.lymph node cells from avn rats tolerant to lew antigens after neonatal induction of tolerance, which did not react in the mixed lymphocyte culture and cytotoxicity assays, were tested for the presence of suppressor cells. sensitization of lymphocytes from normal rats was inhibited by the addition of lymph node cells from these tolerant rats. the mixed lymphocyte culture reaction of lymph node cells from normal avn rats with the irradiated lew cells was inhibited when lymphocytes from tolerant ra ...1978149029
biocompatibility tests of components of an implantable cardiac assist device.a permanently implantable in-series left ventricular assist device, the dynamic aortic patch (dap), has been tested in chronic animal experiments. the dap replaces a section of the intrathoracic aortic wall. hemothorax and hematocele at the implantation site have been complications in recent experiments. primary postoperative hemorrhage was ruled out, and the biocompatibility of all components was therefore examined. dacron velour, teflon felt, conductive polyurethane, segmented polyether polyur ...1978151687
enzyme patterns in single human muscle fibers. 1978152314
high-temperature inactivation of passive potassium transport in electrically non-excitable cells.high-temperature inactivation of passive potassium transport is demonstrated for mouse cell cultures and discussed in analogy to recent results on model systems from phospholipid/cholesterol doted with channel-forming antibiotics.1978154230
deoxyribonucleic acid sequence homologies among bacterial insertion sequence elements and genomes of various organisms.plasmid and phage deoxyribonucleic acid (dna) harboring bacterial insertion sequence (is) elements is1, is2, and is5 were characterized and used as probes to detect homologous sequences in various procaryotic and eucaryotic genomes. the hybridization method used permits the detection of sequences partially homologous to the elements. hybridization of the is-containing probes to each other revealed a region of limited homology between is1 and is2. homologous sequences were then detected by comput ...1979159291
[viral respiratory infections]. 1975162793
cyclic adenosine 3':5'-monophosphate and inhibition of deoxyribonucleic acid synthesis in vitro.results are presented which suggest that inhibition of tritiated thymidine incorporation into the deoxyribonucleic acid of bone marrow in vitro by high concentrations of cyclic adenosine 3':5'-monophosphate is pharmacological in nature, and thus apparently unrelated to the physiological actions of camp.1975165140
hydrophobic interactions of the apo-gln-i polypeptide component of human high density serum lipoprotein.apo-gln-i, the major polypeptide component of human serum high density lipoprotein, has four noninteracting hydrophobic sites which associate with alkanes, anionic detergents, and cationic detergents. hexane and octane bind to these sites with association constants of 6.8 times 10-2 and 1.8 times 10-4 liters/mol, respectively, and compete with the anionic detergent, sodium dodecyl sulfate (c12oso3-minus), at low detergent ligand binding ratios (i.e. smaller than or equal to 1.0 mol of c12oso3-mi ...1975165177
an inherited disorder of persian cats with intracytoplasmic inclusions in neutrophils. 1975166058
cyclic amp and phagocytosis in alveolar macrophages: influence of hormones and dibutyryl cyclic amp. 1975168378
effect of large doses of 131i-19-iodocholesterol on metapyralone-induced adrenal cortical hyperplasia in dogs.the potential use of 131i-19-iodocholesterol to treat acth excess cushing's disease was evaluated in the dog. three normal female dogs were given ld50 radiation doses of 131i-19-iodocholesterol without producing gross or histopathologically demonstrable change of the adrenals at autopsy 3 months later. the adrenal cortices of three dogs were made hyperplastic (to simulate the adrenal cortex in cushing's disease) with acth and three with metapyralone. in addition these six dogs were given kd50 do ...1975170381
the epidemiology and interrelationship of cervical dysplasia and type 2 herpesvirus in a low-income housing project.this report examines the association between cervical dysplasia and herpes simplex virus type 2 antibodies in a low-income housing project, atlanta, georgia. we established a clinic in this project at which women could receive a breast examination and papanicolaou smear and have blood drawn to be tested for herpesvirus type 2 antibodies. prevalence of herpesvirus rose sharply as age increased. there was a significant absence of antibodies in young, nulliparous women. dysplasia showed a tendency ...1975170825
intrauterine pressure wave form characteristics in hypocontractile labor before and after oxytocin administration.the data demonstrate that the contractions of hypocontractile active labor and normal spontaneous labor are different in several measures in addition to maximal amplitude. furthermore, when the pathophysiology is corrected by the use of oxytocin, the contractions resemble those of normal spontaneous labor except in the maximal rate of tension development. our data tend to support the subcellular model of uterine contractility, although the incompleteness of these models limits interpretation.1975170827
information contained in the amino acid sequence of the alpha1(i)-chain of collagen and its consequences upon the formation of the triple helix, of fibrils and crosslinks.the molecule of type i collagen from skin consists of two alpha1(i)-chains and one alpha2-chain. the sequence of the entire alpha1-chain comprising 1052 residues is summarily presented and discussed. apart from the 279 residues of alpha1(i)-cb8 whose sequence has been established for rat skin collagen, all sequences have been determined for calf skin collagen. in order to facilitate sequence analysis, the alpha1-chain was cleaved into defined fragments by cyanogen bromide or hydroxylamine or lim ...1975171554
[effect of the method of cell disintegration on the aspartate kinase activity of preparations from bacillus polymyxa var. ross].the influence of methods of cell disintegration of bac. polymyxa on aspartate kinase activity (ec 2.7.2.4) was examined. the disruption by means of the hews press yielded a more active preparation as compared with ultrasonic disintegration. the supernatant treatment with streptomycin sulphate increased the preparation activity 2-fold. dialysis of the fraction with a high activity of aspartate kinase inactivated the enzyme by 80--85%. the relationship between the aspartate kinase activity and the ...1975171645
human synoviocytes: activation and desensitization by prostaglandins and 1-epinephrine.the human synoviocyte increases its intracellular adenosine 3':5'-cyclic monophosphate (camp) concentration significantly after incubation with prostaglandin e1, prostaglandin e2, or l-epinephrine. the cells can be desensitized to these same hormones. hormone-induced desensitization is receptor site specific and associated with a significant increase in intracellular 3':5'-cyclic amp phosphodiesterase (ec 3.1.4. 17; 3':5'-cyclic amp 5'-nucleotidohydrolase) activity, whereas camp-induced desensit ...1975171653
in vivo electron spin resonance in rats.in vivo esr studies have been made on wistar and sprague-dawley rats using travelling wave helices implanted in the left lobe of the liver. tissue regenerates completely around the teflon-enclosed turns of the coil and histological studies have shown that it is composed of liver cells and connective tissue. electron spin resonance studies of this tissue in vitro reported by commoner and ternberg in 1961 have demonstrated that it exhibits the 'tissue radical' signal. the implanted coils have dete ...1975171686
[the mutagenic effect of methyl methanesulfonate (mms) in vitro and in the host-mediated assay (author's transl)].the concentration- and time-dependent genetic activity of methyl methanesulfonate (mms) was investigated in vitro and in the host-mediated assay using four different indicator organisms. dose/effect curves achieved with the different indicator organisms are similar.1975172045
endotoxic lipopolysaccharides stimulate steroidogenesis and adenylate cyclase in adrenal tumor cells.lipopolysaccharides (endotoxins) from escherichia coli, serratia marcesens and salmonella typhosa stimulated steroid production in y-1 adrenal tumor cells in culture with a latent period of 3-4 h. lipid a, derived from escherichia coli lipopolysaccharide, also stimulated steroidogenesis. lipopolysaccharides and lipid a also stimulate adenylate cyclase activity and cause rounding of the cells. in contrast, lipopolysaccharides do not stimulate steroidogenesis in receptor-deficient adrenal tumor ce ...1975172155
[identification of camp dependant protein kinases in human lymphocytes].chromatographic purification by "deae" cellulose resolves the camp binding proteins in human lymphocytes into three parts. in presence of mg++ each one possesses camp dependent protein-kinase activity, one of them showing allosteric characteristics.1975172248
[resistance to association of antibiotics (polymyxin and penicillin) in relation to cell concentration of two bacterial species in the same inoculum. complementary inoculum effect].dense suspensions of sa. typhi (polys penr) and of st. aureus (polyr pens) proliferate on gelose medium added with the two antibiotics only if the inoculum is mixed. the growth is probably due to the partial neutralization of the antibiotics following the formation of complexes between these antibiotics and some constituents of the two strains.1975172250
hormonal regulation of glycogen metabolism in human fetal liver. i. normal development and effects of dibutyryl cyclic amp, glucagon, and insulin in liver explants.glycogen accumulates in human fetal liver beginning at the eighth week of gestation. a parallel increase in total glycogen synthase activity is found, although the i-form activity remains low and constant throughout the first two thirds of gestation. total phosphorylase activity increases slightly during this period, with the proportion in the active form amounting to about one half of the total throughout. after an initial rapid decline, the glycogen concentration in explants of human fetal liv ...1975172397
plasma adrenocorticoid response to corticotropin in dairy cattle fed high silage diets.responses of plasma corticoids to adrenocorticotropic hormone (.32 u/kg body weight) administered during early lactation were measured in dairy cattle consuming large amounts of concentrate feeds and one of the following forage diets: alfalfa-timothy hay plus corn silage, alfalfa-timothy hay crop silage plus corn silage, or corn silage. concentrations of plasma corticoids increased to maximum in all groups within 60 to 90 min. concentrations remained at near maximum for 4 to 5 h and then decline ...1975172536
effects of quantity and quality of dietary protein and variation in certain enzyme activities on glucose metabolism in the rat.this study attempted to determine whether the quantity and the quality of protein intake could influence the activity of some enzymes involved in carbohydrate metabolism. thus, adult rats were fed for 23 days a diet containing different levels (10 to 70%) and qualities (casein, wheat gluten, and egg yolk) of protein. variations in liver enzyme activities of pyruvate kinase (pk), glucose-6-phosphate dehydrogenase (g6pdh), malic enzyme (me), glucose-6-phosphatase (g6pase), and phosphoenolpyruvate ...1975172617
distribution of membrane-bound cyclic amp-dependent protein kinase in plasma membranes of cells of the kidney cortex.renal cortical plasms membranes were separated by free flow electrophoresis into luminal (brush border microvilli) and contraluminal (basal-lateral membrane) fractions. these membranes were found to contain an intrinsic, self-phosphorylating system which consists of a cyclic amp-dependent protein kinase, a phosphorprotein phosphatase and the substrate(s) of these enzymes. the kinase, but not the phosphatase, was stimulated by cyclic amp; maximal (1.7-fold) stimulation was effected at a cyclic am ...1975172638
properties of the escherichia coli dna-binding (unwinding) protein interaction with nucleolytic enzymes and dna. 1975172646
stimulation of cholesterol ester exchange by lipoprotein-free rabbit plasma.a fraction in normal and hypercholesterolemic rabbit plasma of density greater than 1.25 stimulates the exchange of cholesterol esters between very low density and low density lipoproteins from hypercholesterolemic rabbit plasma. the exchange does not result from lecithin:cholesterol acyltransferase activity. the active factor appears to be a high molecular weight globulin with an isolelectric point of 5.2.1975173390
lysine-sensitive aspartate kinase of serratia marcescens. 1975173646
[state of the horns in cattle in the normal state and in deformations]. 1975174276
a receptor site for prolactin in lactating mouse mammary tissues.prolactin iodinated by lactoperoxidase method showed immunologically, electrophoretically and biolo9gically similar properties to native prolactin and possessed enough specific radioactivity for receptor studies. 1251-prolactin was incubated with mouse mammary tissues at 8 days of lactation. both binding and release of 1251-prolactin depended on incubation time and temperature and were maximal at 37 degrees c. michaelis constant was estimated to be 1.4 x 10(-9) m from lineweaver-burk plot and to ...1975174901
[pathogenic and therapeutic concepts in viral hepatitis]. 1976175890
ultrastructural surface alterations of serratia marcescens after exposure to polymyxin b and/or fresh human serum.exposure of serratia marcescens cells to 10, 5, and 2.5 mug/ml of polymyxin b resulted in outer cell surface alterations that consisted of coarse, pleomorphic projections which revealed a double-contoured membrane structure. in contrast, fresh, but not heat-inactivated human serum caused the deposition of very fine, thread-like aggregates on the outer cell surface of exposed cells. the combination of polymyxin b and fresh human serum caused clearly discernible ultrastructural changes of the poly ...1976176013
effects of cholera enterotoxin, glucagon, and dibutyryl cyclic amp on rat liver alkaline phosphatase, bile flow, and bile composition.cholera enterotoxin, 45 mug per 250 g body weight, administered intravenously to rats, caused a 6-fold rise in the activity of liver alkaline phosphatase in 12 hr. there was no change in bile volume or in the concentration or total bile content of na+, k+, hco3-, or cl- for 36 hr after the administration of cholera toxin. however, bile phospholipid output fell markedly from a control level of 15.0 +/- 1.0 mumol per 6 hr to a low level of 4.0 +/- 1.2 mumol per 6 hr in the 12- to 18-hr collection, ...1976176082
use of horseradish peroxidase to study the antagonism of clostridium welchii (cl. perfringens) type d epsilon toxin in mice by the formalinized epsilon prototoxin. 1976176185
release of xenotropic type c rna virus in response to lipopolysaccharide: acitivity of lipid-a portion upon b lymphocytes.the present studies demonstrate that lipopolysaccharide (lps) causes the release of endogenous xenotropic type-c rna virus from balb/c spleen cells. the evidence suggests that virus release is stimulated by the lipid-a portion of lps and primarily involves an action of lps on b lymphocytes. lps had little or no effect on virus release by t lymphocytes, macrophages, or fibroblasts. these results indicate that the differentiated state of the cell plays an important role in the regulation of endoge ...1976176273
cells isolated from trypsin-treated brain contain trypsin. 1976176319
growth in semisolid agar medium of human cord leukocytes freshly transformed by epstein-barr virus.the semisolid agar method for the selective growth of transformed cells was applied to investigations of epstein-barr virus (ebv) transformation. when 1 x 10(5) human umbilical-cord blood leukocytes were seeded in agar medium immediately after ebv exposure, about 100 colonies developed in each dish. the occurrence of colonies correlated well with dilutions of ebv inoculum. these colonies were composed of lymphoblasts, were positive for ebv-determined nuclear antigen immunofluorescence, and cons ...1976176377
regression of established tumors and induction of tumor immunity by intratumor chemotherapy.the inoculation of a mixture of drugs and guinea pig hepatoma cells (line-10) induced tumor-specific immunity in about 20% of guinea pigs. when guinea pigs with established intradermal tumors were given various drugs ip, no cures were observed; in contrast, multiple intralesional injections of actinomycin d, 1,3-bis(2-chlorethyl)-1-nitrosourea, adriamycin, mitomycin c, and melphalan were effective in curing animals of their intradermal tumors at a time when there were tumor cells in the draining ...1976176411
adenylate cyclase and catecholamine binding in plasma membrane-enriched preparations of cardiac and skeletal muscle.binding of [3h] epinephrine to plasma membrane-enriched fractions from guinea pig heart and rabbit skeletal muscle was investigated using the micropore filtration technique. [3h] epinephrine and [3h] norepinephrine were found to be degraded rapidly in aqueous buffer at ph 7.6 and 37 degrees c. deterioration of the compounds could be prevented by low concentrations of dithiothreitol. binding of [3h] epinephrine to both membrane preparations was a slow process requiring 60 min to approach equilibr ...1976176693
[obtaining the beta-globulin fractions from swine and cattle sera and a study of their immunological activity].the rivanol precipitation was used to obtain beta-globulin fractions from specific swine sera against edema disease, paratyph and aujeszky's disease as well as from normal ovine and swine sera. agar electrophoresis revealed that the preparations produced contained beta-globulin (86 per cent), gamma-globulin (5 per cent), and alpha2-globulin (9 per cent). the beta-globulin preparations were studied for the presence of antibodies against e. coli, salmonellae, staphylococci, myxovirus parainfluenza ...1976176771
[synthesis of virus-specific macromolecules and virion maturation in chick embryo cells infected with sendai virus].synthesis and accumulation of virus-specific rna, proteins and intracellular nucleocapsids are observed in sendai virus-infected chick embryo cells for approximately 2 days postinfection. however, only an insignificant part of these products leaves the cell as a result of maturation of virus particles. it is suggested that the cause of the ineffective maturation of virions in the system under study may be in the observed instability of one of virus structural proteins in the infected cells, m pr ...1976176819
effects of potassium chloride and smooth muscle relaxants on tension and cyclic nucleotide levels in rat myometrium.ten minutes after kcl-depolarization of rat myometrial strips, at which time the muscles were in a state of sustained contracture, tissue levels of adenosine 3',5'-cyclic monophosphate (cyclic amp) were increased by approximately 40% over relaxed controls, and levels of guanosine 3',5'-cyclic monophosphate (cyclic gmp) were decreased by 40%. at this point both nitroglycerin (4 x 10(-4) m) and papaverine (2 x 10(-5) m) were capable of relaxing the depolarized muscles without significantly increas ...1975177160
hepatomas in grs/a male mice treated with horse serum or horse anti-mouse antilymphocyte serum. 1976177290
investigations into the mechanism of reduction of ethanol sleep by thyrotropin-releasing hormone (trh).thyrotropin-releasing hormone (trh), administered intraperitoneally, was found to antagonize ethanol-induced sleep and hypothermia in mice without affecting brain ethanol content. this reduction of the actions of ethanol was also apparent after oral or intracisternal administration of trh. in addition, trh reduced ethanol-induced sleep in rats, hamsters, gerbils and guinea pigs. evidence that the pituitary-thyroid axis is not necessary for the effects of trh was provided by observations that hyp ...1976177753
sulfamethoxazole-trimethoprim-polymyxin therapy of serious multiply drug-resistant serratia infections.nonpigmented multiply drug-resistant serratia marcescens caused an extensive outbreak of infection at the nashville veterans administration hospital. isolates were of one serotype resistant to all currently available antimicrobial agents for therapy of systemic infections except for occasional susceptibility to chloramphenicol and kanamycin. frequently strains were susceptible to nalidixic acid, and all were susceptible to amikacin (bb-k8). drug-resistant strains caused 130 infections, 12 bacter ...1976178273
[traumatic deciduoma and delayed nidation in castrated pregnant rat after acth injection].after administration of acth it is possible to obtain traumatic deciduomata in rats ovariectomized during early pregnancy and delayed implantation in rats ovariectomized day 4 of pregnancy and injected dayly with oestradiol from day 8 or 9. the substance responsible seems to be progesterone or progesterone like corticoid.1975178414
evidence of methylation of b77 avian sarcoma virus genome rna subunits.b77 avian sarcoma virus rna was labeled with (methyl-3h) methionine under conditions that prevent non-methyl incorporation of 3h radioactivity into purine rings. from the determined values for the extent of methylation of 4s rna isolated from infected chicken embryo cells, it was estimated that 30 to 40s rna subunits that results from heat denaturation of the 60 to 70s rna contain approximately 21 methyl groups, of which 14 to 16 are present at internal positions as n6 -methyladenosine residues. ...1976178899
studies on the inhibitory activities of human serum lipoproteins for japanese encephalitis virus.human serum lipoproteins were purified by ultracentrifuging and their concentrations adjusted as required to be within the normal male/female serum range for all assays. the activities in inhibition of hemagglutination (hai) for japanese encephalitis virus were--low density lipoprotein (ldl) greater than very low density lipoprotein (vldl) greater than high density lipoprotein (hdl). heating (56 degrees c/30 minutes) caused the ldl titer to fall and freeze-thawing (20 degrees c/room temperature) ...1975179150
an experimental model for disseminated herpesvirus infection of the neonate.pregnant mice at 10 days of gestation were more susceptible to vaginal inoculation with herpesvirus, type 2, than were nonpregnant mice. this mouse model system also was used to compare delivery through a birth canal contaminated with herpesvirus, type 2, to delivery after maternal paranteral inoculation by the same virus. approximately 26 per cent of newborn mice either died or had evidence of disseminated herpesvirus infections after delivering through a vagina inoculated with herpesvirus, whe ...1976179321
optimum conditions for the binding of insulin to its receptor.the specific binding of insulin to the membranes from lactating mouse mammary gland was studied as a model of hormonereceptor type of binding. the basic ingredients of binding, the concentration of receptor protein and the concentration of labeled insulin were mainly studied. the characteristic changes in specific binding were followed, the adequate regression equation was draen and the optimum conditions of binding were established for further experiments. the expediency of applying shortened o ...1976179574
technetium-99m labelled imidodiphosphate: an improved bone-scanning radiopharmaceutical.technetium-99m labelled imidodiphosphate was prepared in the presence of stannous ions. it was evaluated as a bone-scanning agent in animals and patients. comparative tissue distribution studies in mice showed a relatively higher uptake of radioactivity in bone when 99tcm -labelled pyrophosphate and diphosphonate. accumulation of radioactivity in soft tissues, especially kidneys, was less with this radiopharmaceutical. results in patients were most satisfactory in delineating skeletal bone and i ...1976179654
steroid production by quartered and capsular rat adrenal gland in response to haemorrhage.60 min after rapid bleeding (1.5--2.0 per cent of b. w.) both aldosterone and corticosterone production rate by quartered rat adrenals were found to be elevated. however, no difference was observed in the rate of aldosterone and corticosterone production by capsular adrenals of sham operated and hypovolaemic rats. corticosterone production rate by decapsulated adrenals was much more higher after haemorrhage than in the control group. the same alterations could be observed incubating adrenal tiss ...1975179800
cytokinin ocntents and camp metabolism during growth of escherchia coli.during escherichia coli growth, we found an inverse correlation between free cytokinin content and camp level. the rates of synthesis of adenylate-cyclase and camp-phosphodiesterase were practically constant.1976179840
[changes in the functional state and structure of the adrenals in cc14 poisoning].introduction of cci4 in doses of 0.1 and 0.2 ml/100 g to albino rats reduced the steroid production generated by adrenal sections. in 24 and 48 hours after poisoning with a small dose of cci4 (0.1 ml/100 g) there could be seen an increased concentration of 11-oxycorticosteroids in the plasma. morphological signs of a damage suffered by the gland were observed only in poisoning with a large dose of cci4 (0.2 ml/100 g).1975179852
lipid-protein interaction in porcine high-density (hdl3) lipoprotein. 1975179867
nad+-malic enzyme. regulatory properties of the enzyme from ascaris suum.the regulatory properties of the nad-dependent malic enzyme from the mitochondria of ascaris suum have been studied. the malate saturation curve exhibits sigmoidicity and the degree of this sigmoidicity increases as the ph is increased. fumarate was the only compound tested that stimulated the enzyme activity, whereas oxalacetate was the most powerful inhibitor. activation by low levels of fumarate was found to be competitive with malate. it is proposed that this stimulation has physiological si ...1976180002
stimulation of ornithine decarboxylase synthesis and its control by polyamines in regenerating rat liver and cultured rat hepatoma cells.ornithine decarboxylase has been induced in log phase hepatoma cells grown in suspension culture. induction with n6, o2'-dibutyryl cyclic adenosine 3':5'-monophosphate produced a 4-fold increase in enzyme activity by 3 hours which was followed by a return to base levels by 6 hours. induction with dexamethasone, a potent synthetic glucocorticoid, exhibited a slow steady rate of increase in enzyme activity, reaching a plateau level of approximately 5- to 6-fold stimulation by about 12 hours. indu ...1976180026
in vivo reversal of thyroxine induction of dna synthesis by dibutyrly cyclic amp in developing rat cerebellum.thyroxine, dibutyryl cyclic amp, and a combination of both drugs were administered daily from birth to 2, 2 and 3 pups, respectively from each of 5 litters of sprague-dawley rats. body weight, brain weight, cerebellar weight, and cerebellar dna were measured in each animal at age 5 days and compared with values from a pair of controls from each litter. cerebellar weight and dna content were affected more severely than body weight in cyclic amp-treated animals, with cerebellar dna reduced signifi ...1976180263
experimental alterations in cyclic adenosine monophosphate concentrations in the cat basilar artery.levels of cyclic adenosine monophosphate (amp) in the basilar artery and in circulating blood of cats were determined after the production of spasm by topical application of blood to the vessel and following treatment with agents known to alter cyclic amp. isoproterenol, known to stimulate adenyl cyclase, and aminophylline, a phosphodiesterase inhibitor, were studied alone and in combination. cyclic amp of the basilar artery fell from a mean control value of 43 to 26 pmoles per milligram of prot ...1976180456
serum testosterone and dihydrotestosterone in patients with trophoblastic disease.serum testosterone (t) and dihydrotestosterone (dht) were measured by radioimmunoassay in 14 patients with unaborted hydatidiform mole and in 16 patients with normal pregnancy of similar gestational age. serum human chorionic gonadotropin (hcg) was measured by the radioreceptor assay in patients with hydatidiform mole. serum t ranged from 0.27 to 5.39 ng/ml with a mean +/- se of 2.21 +/- 0.45 ng/ml in patients with hydatidiform mole mole and from 0.20 to 2.40 ng/ml with a mean +/- se of 0.80 +/- ...1976180465
calcium-binding protein and calcium absorption in the laying quail (coturnix coturnix japonica).a vitamin d3-dependent calcium-binding protein (cabp) has been found in the intestinal mucosa of japanese quail (coturnix coturnix japonica). this protein is similar, if not identical to that of the chick (gallus domesticus). a similar protein fraction appears also in uterine mucosa of laying quail. both intestinal and uterine cabp levels are higher in laying than in non-laying quails. uterine cabp was higher during egg shell formation than during uterine inactivity. the slight corresponding dif ...1976180508
a fatty acyl-coa oxidizing system in rat liver peroxisomes; enhancement by clofibrate, a hypolipidemic drug.purified rat liver peroxisomes contain a cyanide-insensitive fatty acyl-coa oxidizing system that uses o2 and nad as electron acceptors. the system was detected by the ability of added palmitoyl-coa to elicit o2 consumption, h2o2 production, and o2-dependent nad reduction. the activity of this system is increased approximately one order of magnitude in rats treated with clofibrate, a hypolipidemic drug known to cause peroxisomal proliferation.1976180535
[binding to chicken liver lactatedehydrogenase (author's transl)].some information about the lactate dehydrogenase nad binding site has been obtained by working with coenzymes analogs of incomplete molecules. 5'amp, 5'-adp, atp, 5'-c-amp and 3'(2)-amp inhibit chicken liver ldh activity competitively with nadh. 5"-amp and 5'-adp show a stronger inhibition power than atp, suggesting that the presence of one or two phosphate groups at the 5' position of adenosine, is essential for the binding of the coenzyme analogs at the enzyme binding site. ribose and ribose-5 ...1976180571
[epidemiological aspects of cytomegalovirus infections]. 1975180573
characterization of a nosocomially significant, multiple drug-resistant strain of serratia marcescens.a multiple drug-resistant strain of serratia marcescens (bacteriocin type 18) was isolated from three clinical patients. the isolates were found to carry a conjugally nontransferable, nonmobilizeable resistance plasmid (r-plasmid) with resistance-(r)-determinants against ten antimicrobial drugs: ampicillin, carbenicillin, chloramphenicol, gentamicin, kanamycin, neomycin, streptomycin, tobramycin, triple sulfonamides, cotrimoxazole, and--possibly--nalidixic acid, as determined with exposure to 'c ...1976181215
stimulation of lymphocyte proliferation by killed mycobacteria and other bacterial species.killed mycobacteria and some mycobacterial fractions induced spleen cells from normal c3h mice to incorporate tritiated thymidine to a relatively high degree. thymocytes under the same conditions were not activated. however, incorporation of thymidine was significantly increased when mixtures of thymus and spleen cells were cultured in the presence of the inducers. a similar activation of spleen cells was displayed in the presence of gram-positive and gram-negative non-mycobacterial species.1976181325
in vivo activation by polymyxin b of phospholipase from pseudomonas aeruginosa and escherichia coli. 1976181357
proteoglycan complexes from bovine heart valve. fractionation by density-gradient centrifugation and gel filtration under dissociative conditions.proteoglycan complexes from collagenase [ec 3.4.24.3]-indigestible materials of bovine heart valves were extracted with 4 m guanidinium chloride, purified by ion-exchange column chromatography in a urea-containing solution, then fractionated by density-gradient centrifugation under dissociative conditions. electrophoretic characteristics and enzymic susceptibility of the density-gradient fractions revealed that the glycosaminoglycans constituting the proteoglycan complexes in this indigestible m ...1976181367
thyroid antigen stimulates lymphocytes from patients with graves' disease to produce thyroid-stimulating immunoglobulin (tsi).circulating lymphocytes from patients with graves' disease and from control subjects were cultured in vitro alone, with normal human thyroid tissue homogenates, and with other nonthyroid human tissue homogenates. the supernatants of these cultures were assayed for human thyroid-stimulating activity by incubation with human thyroid slices in which increases in camp levels were then measured. human thyroid stimulator activity was demonstrated in 16 out of 20 experiments in which lymphocytes from p ...1976181397
thyroid peroxidase, thyroglobulin, camp and dna in human thyroid.tissues from normal thyroid, euthyroid multinodular goiter, toxic goiter, hashimoto's thyroiditis, thyroid adenoma, and carcinoma were examined for their content of deoxyribonucleic acid (dna), thyroid peroxidase activity (tpa), thyroglobulin (tg), and adenosine 3',5'-cyclic monophosphate (camp). compared with normal thyroids dna was increased in the neoplastic tissues and decreased in euthyroid multinodular goiter. quantitative changes of thyroglobulin were observed, especially in the thyroid t ...1976181398
structure of herpesvirus saimiri genomes: arrangement of heavy and light sequences in the m genome.herpesvirus saimiri contains two species of dna molecules. (i) the m genome is composed of 70% light (l) dna (36% cytosine plus guanine; density in cscl, 1.695 g/ml), which consists of unique sequences, and 30% heavy (h) dna (71% cytosine plus guanine; density, 1.729 g/ml). (ii) the h genome contains heavy sequences exclusively. h sequences in m and h genomes cross-hybridize completely and are cleaved identically by restriction endonuclease r-sma i into four classes of fragments with molecular w ...1976181593
factors influencing the size, composition and metabolism of very low density lipoproteins in the rat. 1976181699
a protein-binding assay for direct determination of adenosine 3',5'-monophosphate in amniotic fluid, cerebrospinal fluid, plasma, and urine.an adenosine 3',5'-monophosphate (cyclic amp)-binding protein was isolated from bovine skeletal muscle. this preparation showed maximum binding capacity for cyclic amp at the physiological ph of amniotic fluid, cerebrospinal fluid, and plasma and had a high association constant of 1.4x10(9) 1/mol. this preparation of binding protein, together with albumin and edta in the assay buffer, gave a sensitive and specific competitive protein-binding assay that permitted direct determination of cyclic am ...1976181838
enzootic with ectromelia symptomatology in sprague-dowley rats. 1976181904
binding of digitoxin and digoxin to normal human beta-lipoproteins. 1976181946
specific modification of the gtp binding sites of rat 5'-adenylic acid aminohydrolase by periodate-oxidized gtp.1. rat skeletal muscle amp deaminase (amp aminohydrolase, ec3.5.4.6) can be inactivated by incubation with the periodate-oxidized analogue of the enzyme inhibitor gtp. 2. nucleoside triphosphates and kcl at high concentrations protect against inactivation, while adp has no effect. 3. the inactivation can be reversed by the addition of gtp and amino acids and made irreversible by reduction with nabh4. this indicates that, in the binding of the oxidized gtp to the enzyme, a schiff base is formed b ...1976182275
rd-114, baboon, and woolly monkey viral rna's compared in size and structure.the molecular weights, subunit compositions, and secondary structure patterns of the rnas from an endogenous baboon virus and from a woolly monkey sarcoma virus were examined and compared to the properties of the rna of rd-114, an endogenous feline virus. the high molecular weight rna extracted from each of these three viruses has a sedimentation coefficient of 52s, and a molecular length, measured by electron microscopy, of 16-20 kb (kb=kilobase, 1000 nucleotides). each such rna is a dimer, con ...1976182377
diagnostic tests of bovine leukemia: comparison between an hematological test and the serological diagnosis. 1976182441
effectiveness of orally administered bordetella pertussis vaccine in mice.oral administration of killed bordetella pertussis organisms to mice results in increased resistance to an intracerebral infection with virulent b. pertussis cells. the rate of survival is dependent on the dose of antigen. but besides specific systemic immunity, which is persistent over a long period, also transient non-specific resistance is increased. these effects are evidently induced without penetration of bacterial substances into the circulation.1976182602
[protective effect of cytochrome c in carbon tetrachloride poisoning]. 1976182619
evidences for complex formation between polymyxin b and lipopolysaccharides from serratia marcescens.in vitro and in vivo complex formations of polymyxin b and lipopolysaccharides (lps) from resistant and sensitive cells of serratia marcescens were studied by polyacrylamide gel electrophoresis in sodium dodecyl sulfate and electron microscopy. in vitro treatment of lps from resistant cells with polymyxin b gave two populations of spherical complexes of differnt molecular weights as determined electrophoretically. similar treatment of lps from sensitive cells resulted in dissociation of the lps- ...1976182663
effect of norepinephrine and hypertonicity on k influx and cyclic amp in duck erythrocytes.cyclic adenosine 3',5'-monophosphate (camp) accumulation and cation transport were measured in duck erythrocytes after stimulation by norepinephrine (ne) or shrinkage induced by exposure to hypertonic media (s). previously both ne and s were shown to initiate a similar transport process in this cell. ne elicited a rapid rise in cellular camp and 42k influx. both effects were eliminated by propranolol. at concentrations of ne below 3 x 10(-8) m (the concentration at which 42k influx saturates), t ...1976183515
differential inactivation of the "l" and "ly+" amino acid transport systems by a sulfhydryl reagent and a photo-affinity probe.using a substrate-stimulated amino acid efflux system, it has been shown that the "ly+" and "l" amino acid transport systems of mouse embryo cells in culture are differentially inhibited by parachloromercuribenzene sulfonate (pcmb-s) and the photoaffinity probe 4-fluoro-3-nitrophenylazide (fnpa). three types of evidence support the conclusion that these transport systems are mediated by separate carrier proteins. (1) the specificity of substrate-stimulated efflux is high for each system; (2) pcm ...1976183824
susceptibility of "enterobacteria" to aminoglycoside antibiotics: comparisons with tetracyclines, polymyxins, chloramphenicol, and spectinomycin.strains of enterobacteria, including common, aerobic, pathogenic gram-negative bacilli, and enterococci were tested for susceptibility to 11 aminoglycoside antibiotics by a twofold agar-dilution method with an inocula replicator. for comparison, similar tests were done with seven tetracycline analogues, two polymyxins, chloramphenicol, and spectinomycin. tobramycin compared favorably with the more active aminoglycosides, with some exceptions related to individual strains of species. the tetracyc ...1976184213
methionine-repressible homoserine dehydrogenase of serratia marcescens: purification and properties.serratia marcescens sa-3 possesses two homoserine dehydrogenases and neither has any aspartokinase activity unlike the case of escherichia coli enzymes. the two enzymes have been separated. one of them is active with either nad+ or nadp+ and has been purified about 180-fold to homogeneity. this enzyme is completely repressed by the presence of 1 mm methionine or homoserine in the growth medium, but its activity is unaffected by any amino acid of the aspartate family either singly or together. in ...1976184374
haemorrhagic and inflammatory properties of collagenase from c. histolyticum.collagenase from clostridium histolyticum induced haemorrhages when applied to the surface of dog lung; it exerted a similar effect on mouse lung when injected intrathoracically. injected into rat paws, bacterial collagenase induced haemorrhage and oedema. effects of collagenase were prevented by several procedures that inhibit collagenolytic activity (heating at various temperatures and incubation with metal-complexing agents such as edta, penicillamine and dithiothreitol). protein protease inh ...1976184702
urea, a specific inhibitor of catabolite sensitive operons. 1976184780
plaque formation with influenza viruses in dog kidney cells.infectious influenza and parainfluenza viruses were produced as plaql passages of egg-grown viruses in mdck monolayers. virus titres of 10(6) to 10(11) p.f.u./ml were obtained for several a and b strains of influenza and parainfluenza viruses.1976185324
enumeration of antigen reactive t cells against sheep erythrocytes by the virus plaque assay. 1976185443
integration of biochemical functions of different cells of rat gastric mucosa for hydrochloric acid secretion.the regulation patterns of gastric acid secretion in rats were investigated. pentagastrin and histamine stimulate gastric acid secretion, but the inhibitors of dna-dependent synthesis of rna and of proteins prevent only the pentagastrin action. it has been found that pentagastrin induces histidine decarboxylase in gastric mucosa, ensuring local accumulation of histamine. the latter activates adenylate cyclase and results in 3',5'-amp accumulation in gastric tissues. the administration of pentaga ...1976185510
opiate receptor: autoradiographic localization in rat brain.opiate receptor sites in rat brain can be labeled in vivo by [3h]diprenorphine, a potent opiate antagoinst. using techniques to minimize diffusion in fresh, frozen, unfixed brain, we have localized [3h]diprenorphine by autoradiography to visualize the distribution of opiate receptors. silver grains indicative of the binding of labeled [3h]diprenorphine are discretely localized in numerous areas of the brain with very high densities in the locus coeruleus, the substantia gelatinosa of the spinal ...1976185626
[pathogenesis of spontaneous diabetes mellitus in the cat. ii. acidophilic adenoma of the pituitary gland and diabetes mellitus in 2 cases]. 1976186021
genetic factors influencing endogenous type-c rna viruses of mouse cells: control of viral polypeptide expression in the c57bl/10 strain.type-c viral polypeptides are expressed at very low levels in c57bl/10 liver and embryo cells as compared to cells of other mouse strains. the 12,000-mol. wt. polypeptide in c57bl/10 cells was purified and shown to possess type-specific antigenic determinants indistinguishable from those of a previously described class of endogenous virus. the very low levels of endogenous virus-specific rna in cells of c57bl/10 as compared to other strains suggests control of this endogenous virus at the level ...1975186435
[mechanism of internal inhibition (role of the sympathetic nervous system in sleep phenomena)].it has been shown on cats that the role of the sympathetic nervous system in sleep phenomena is of an adaptive nature and is effected through chain neurohormonal reactions in which adrenaline possessing a two-phase action plays a major part: it induces excitation followed by sleep. sleep is regarded as a variety of internal inhibition, elaborated as an adaptive reaction to the changing conditions of environment, which later becomes a behavioral reaction.1976186977
long-term effects of neonatal steroid exposure on mammary gland development and tumorigenesis in mice.newborn female mice of three strains--balb/cfc3h [mammary tumor virus (mumtv)-infected], balb/c, and c57bl (both virus-free)--were given injections of 17beta-estradiol or testosterone, alone or in combination with ovine prolactin, for the first 5 days of life. half of each group of mice were ovariectomized at 40 days of age, and all mice were killed between 6 and 16 months of age. mammary glands of balb/cfc3h mice receiving steroid hormones were better developed than those of mice not receiving ...1976187788
dna-relaxing activity and endonuclease activity in xenopus laevis oocytes.complex simian virus 40 dna produced by a soluble cell-free extract derived from stage 6 oocytes of xenopus laevis consists of fully relaxed circles (i.e., with no superhelical turns). an endonuclease and a dna-relaxing protein, either or both of which could be responsible for the relaxation of the complex dna, have been purified from the extract. the endonuclease(s) produces nicked circles (having a single-strand scission) and linear full-size molecules. the dna-relaxing protein is in the nucle ...1976188045
[the concept of risk in poliomyelitis (author's transl)].in poliomyelitis the risk is of neurological accidents resulting either from the circulation of wild viruses (spontaneous risk) or from the introduction of virus-vaccine (vaccine risk). the spontaneous risk varies both according to the virus type and according to various factors which determine host resistance. well known among these are: the intrinsic characteristics of the population at a given period (genetic factors and previous experiences of this population with poliomyelitis viruses); env ...1976188083
[spondylodiskitis caused by klebsiella pneumoniae, an unusual complication of diverticular sigmoditis]. 1976188196
[synthesis of virus-specific rna under conditions of homologous and heterologous interference by newcastle disease virus].homologous and heterologous interference by newcastle disease virus (ndv) was manifested in a marked inhibition of virus-specific rna synthesis of both the interacting viruses. inactivation of the interfering ndv by irradiation reduced its capacity to inhibit the synthesis of virus-specific rna of the homologous virus, leaving its effect on the synthesis of rna of the heterologous virus unchanged. in contrast, treatment of cell cultures with heparin leading to reduced interferon production elimi ...1976188251
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