in vitro proliferation of macrophage depleted human peripheral blood lymphocytes.the incorporation of thymidine by normal human peripheral blood lymphocytes was tested in vitro following various culture conditions. a significant increase of thymidine uptake was observed in cultures depleted of plastic adherent, nylon wook adherent, or phagocytic cells. this proliferative activity occurred in the presence of various sera but was not due to a blastogenic response to a foreign protein, since it was also observed when autologous plasma was the only source of protein in the cultu ...1979535185
[pharmacokinetics of a new cephalosporin, cefoperazone].cefoperazone is a semi-synthetic cephalosporin for parenteral use with an extended antibacterial spectrum covering pseudomonas aeruginosa, enterobacter cloacae and serratia marcescens. its pharmacokinetic properties were studied in 8 healthy subjects after 2 intravenous infusions of 2 g of the drug at a 12-hour interval. the mean peak serum concentrations were 134 +/- 16 microgram/ml and 143 microgram/ml. cefoperazone was shown to possess a long half-life for a cephalosporin (1.7 hours). in our ...1979538442
histochemical studies on peroxisomes in regenerating proximal tubules of the kidney.peroxisomes of the regenerating proximal tubules of the rat kidney were investigated after necrosis induced by mercuric chloride. slices both for light and electron microscopic examinations were incubated in 3,3'-diaminobenzidine (dab) medium. peroxisomes were absent in the necrotic epithelium. they appeared on the fourth day of regeneration and later their number increased reaching the normal distribution in the fourth week. they seem to be needed for the functional differentiation of the proxi ...1979540103
[basophil "degranulation" as an index of the body stress state].a regular "degranulation" development of basophilic leucocytes in the blood has been established not only in cases of the joint and skin allergic reactions but in acute and chronic experimental hemorrhage, skin burns and acute radiation disease. basophilic "degranulation" denotes the stress state of the organism as a result of stress situations.1979540155
percutaneous absorption of dodecyltrimethylammonium bromide, a cationic surfactant, in the rat. 1979546698
intoxication of cells from different-aged embryos by diphtheria toxin.different methods were used to assay diphtheria toxin sensitivity of fibroblast and heart cell cultures from chicken embryos of various ages. as defined by inhibition of protein synthesis, fibroblasts from 18-day and younger embryos respond more rapidly to toxin than fibroblasts from older embryos. the response of heart cells cultures is independent of the age of the embryos and is similar to the response of fibroblasts from 18-day embryos. since the ef-2 content is 10-fold less in fibroblasts f ...1979546782
interaction of morphine and 5-hydroxytryptamine in the raphe-hippocampus order to describe the interaction of morphine and 5-hydroxytryptamine (5-ht) in the raphe-hippocampus system we tested the influence on the antinocifensive effect of topic administrations of morphine and serotonergic substances into the dorsal hippocampus and the median raphe nucleus in rats. 5-ht administered into the dorsal hippocampus increased the morphine analgesia. lysergic acid diethylamide injected into the raphe nucleus antagonized the morphine effect. morphine given into the raphe n ...1979547279
[influence of tobacco smoking on caffeine pharmacokinetics (author's transl)]. 1979547440
[antibacterial kinetics of fosfomycin (author's transl)].the authors studied, with the autobac machine, the kinetics of antibacterial activity of fosfomycin against staphylococcus aureus, streptococcus d' streptococcus pneumoniae, neisseria meningitidis, acinetobactor lwoffi, haemophilus influenzae, salmonella typhimurium, escherichia coli, proteus rettgeri, klebsiella pneumoniae, serratia marcescens and pseudomonas aeruginosa. a correlation appears between the kinetics of fosformycin antibacterial action and the microbial growth rate.1979547759
[thermoregulation in chickens subjected to hypoalimentation].calculation of the body temperature of 4 chickens, 14 days old, submitted during 26 days to a reduced nourishment so that their growing up was stopped. morning temperature diminished, in comparison with checkings, of 0,78 degrees in the first week, of 1,57 degrees in the second week and of 1,80 degrees in the last days. after 1 and 2 hours of reduced meal, the temperature generally increases 0,76 degrees and 1,39 degrees respectively when chickens are hypo-nourished, while in the checkins it is ...1979548058
[histochemical studies of the epithelium of the human ampulla ductus deferentis].human ampullary epithelium contains numerous apical granules, which show a strong p.a.s. reaction, but do not stain with methods for acid mucins. no glycogen is detected. acid phosphatase and non-specific esterase are localized in granules, scattered within the cytoplasm, while peroxidase activity is not demonstrable. the significance of these results is discussed.1979548072
enterobacteria of emerging pathogenic significance from clinical cases in man and animals and detection of toads and wall lizards as their reservoirs.a total of 416 samples comprising faecal samples from diarrhoeic cases of man, calves, sheep and goats, and urine samples from patients with urinary tract infections, were examined for the presence of enterobacteria of emerging pathogenic significance. citrobacter freundii from 20, c. intermedius biotype-a from four, serratia marcescens (serotype 05:h13, bactericin type 16) from one and erwinia herbicola from two human stool samples were isolated. only two urine samples yielded c. freundii. citr ...1978582096
laboratory studies on mecillinam: activity alone and combined with cephradine.mecillinam (mc) was found to be inhibitory at low concentrations to a wide range of gram-negative bacteria; the sensitivity of such strains could be predicted by using a 10 microgram disk. resistance to mc did not appear to be associated with beta-lactamase-mediated destruction of the antibiotic. ampicillin-resistant strains tended to be less sensitive to mc. mc and cephradine acted synergistically against proteus mirabilis, pr. vulgaris, prov. stuartii and serratia marcescens, and the combinati ...1977587708
microbial conversion of dl-2-amino-delta2-thiazoline-4-carboxylic acid to l-cysteine and l-cystine: screening of microorganisms and identification of products.microorganisms able to form l-cysteine from dl-2-amino-delta2-thiazoline-4-carboxylic acid (dl-atc), a chemical intermediate in the synthesis of dl-cysteine, were isolated from soil samples and classified as pseudomonas sp., pseudomonas cohaerens, p. desmolytica, and p. ovalis. thirteen l-cysteine-producing bacteria were also found in among 463 stock cultures representing 37 genera. these were achromobacter delmarvae. alcaligenes denitrificans, bacillus brevis, brevibacterium flavum, enterobacte ...1977596877
clinical study of the use of the new aminoglycoside tobramycin for therapy of infections due to gram-negative bacteria.tobramycin, a new aminoglycoside antimicrobial, was evaluated as therapy for infections due to gram-negative bacilli in 15 seriously ill patients with underlying diseases. three of 10 patients with gram-negative bacteraemia also had a urinary tract infection. two patients had a respiratory tract infection, one had cellulitis, one had a urinary tract infection, and another had septic phlebitis. twelve patients were cured of their infections. eosinophilia was observed in one patient, and another d ...1977600202
treatment of mediastinitis in children after cardiac surgery. a study of 20 cases.twenty-three cases of mediastinitis after cardiac surgery in children were treated by us between 1973 and 1976. three patients died within 6 hours of admission. treatment used in the tweny other cases are discussed. the mean age of the patients was three years and three months. the mediastinitis was evident an average of twelve days after extracoporeal circulation. a staphylococus was always responsible for the infection. treatment was a combination of surgery, antibiotics and respiratory and nu ...1978621313
pc-904, a new semisynthetic penicillin.sodium 6{d(-)-alpha(4-hydroxyl-1,5 naphthyridine-3-carboxamido)phenylacetamido} penicillanate (pc-904) is a new semisynthetic penicillin with broad-spectrum activity against gram-positive cocci and gram-negative bacilli. at a concentration of 1.56 mug/ml, it inhibited 100% of isolates of proteus mirabilis, 89% of pseudomonas aeruginosa, 67% of escherichia coli, and 45% of enterobacter spp. at a concentration of 12.5 mug/ml, it inhibited 75% of klebsiella spp. and 67% of serratia marcescens. pc-9 ...1978626486
sisomicin: in vitro activity and vitro activity of sisomicin and gentamicin was compared in serial dilution tests for 619 bacterial strains (staphylococcus aureus, e. coli, proteus mirabilis, proteus vulgaris, enterobacter, klebsiella, pseudomonas aeruginosa, salmonella, serratia marcescens). mean mic of sisomicin was lower by one geometrical dilution step compared with gentamicin for pseudomonas, proteus vulgaris, klebsiella, and serratia, while it was almost identical with the other species. resistance (mic greater than 5 ...1978649233
distortion of endothelial repair. the effect of hypercholesterolaemia on regeneration of aortic endothelium following injury by endotoxin. a scanning electron microscope study.five young male new zealand white rabbits were fed a semi-synthetic diet containing 0.2% cholesterol for 2 weeks and a control group of 5 animals was fed a normal stock diet. all animals were then injected intravenously with a single dose of endotoxin from serratia marcescens (200 microgram/kg body weight) and continued on their respective diets for a further 4 weeks. the aortas were then stained with silver nitrate and fixed under pressure for scanning electron microscopy (sem). argyrophilic en ...1978666889
in vitro studies of piperacilin, a new semisynthetic penicillin.piperacillin, a new semisynthetic penicillin, was compared with other semisynthetic penicillins, cephalosporins, and aminoglycosides by the agar dilution method against 3,600 isolates of facultative gram-negative bacilli, bacteroides fragilis, and enterococci. at 64 mug/ml, piperacillin inhibited 90% of the isolates in each group of organisms tested except for escherichia coli (83% inhibited by 64 mug/ml). compared with carbenicillin, piperacillin had a 16-fold increase in activity by weight aga ...1978677861
in vitro activity and beta-lactamase stability of bl-s786 compared with those of other vitro activity of bl-s786, a new parenterally semisynthetic cephalosporin, was investigated against 570 bacterial isolates. bl-s786 inhibited most escherichia coli, klebsiella pneumoniae, proteus mirabilis, and salmonella. it inhibited some enterobacter and indole-positive proteus, but it was less active against these later species than was cefamandole, cefuroxime, or cefoxitin. it was not active against serratia marcescens, pseudomonas aeruginosa, or bacteroides fragilis. bl-s786 was the lea ...1978686701
stimulation of polymorphonuclear leukocyte bactericidal activity by supernatants of activated human mononuclear cells.supernatants of phytohemagglutinin-activated human mononuclear cells stimulated polymorphonuclear leukocyte (pmn) activity against the gram-negative organism serratia marcescens. in the absence of serum opsonins, when control pmn could not impede bacterial growth, stimulated pmn averaged more than 0.6-log kill of the original bacterial inoculum. in the presence of optimal amounts of serum opsonins, when control pmn were significantly bactericidal, stimulated pmn killed, on the average, at least ...1978730369
use of cefoxitin, new cephalosporin-like antibiotic, in the treatment of aerobic and anaerobic infections.forty-two patients were treated with intravenous cefoxitin, a new cephamycin antibiotic. these patients had postoperative abdominal sepsis (26), intrathoracic infections (6), urinary tract infections (5), gram-negative bacterial meningitis (2), septic arthritis (1), epidural abscess (1) and isolated septicemia (1). the antibacterial spectrum of cefoxitin was found to be one which included all gram-positive organisms except enterococci, most gram-negative organisms except pseudomonas aeruginosa, ...1978742874
radiosensitization of serratia marcescens by cetylpyridinium chloride. evidence for membrane-associated events.cetylpyridinium chloride has been shown to be an effective radiosensitizer of both oxic and anoxic suspensions of serratia marcescens in buffer. the related compounds ethylpyridinium bromide and cetyltrimethylammonium chloride exhibited no such radiosensitizing properties at comparable concentrations. it is suggested that the efficiency of cetylpyridinium chloride is due to the combination of lipid-soluble (cetyl) and electron-affinic (pyridinium) moieties within the same molecule, and that thes ...1976766063
proceedings: fast mixing studies on the time scale of the oxygen effect in irradiated bacteria. 1975766802
proceedings: fast repair of oxygen-dependent lethal damage in irradiated bacteria. 1975766887
in vitro sensitivity of hospital strains of serratia marcescens to chemotherapeutic agents.the susceptibility of 83 non-pigmented serratia marcescens strains was determined by an agar dilution technique. they originated from miscellaneous pathological specimens submitted to the diagnostic laboratory during a nosocomial infection outbreak in 1974. all strains were completely resistant to 128 mug/ml of cephalothin, colistin sulphomethate, lincomycin and penicillin g. they were also resistant to clinically attainable concentrations of ampicillin, chloramphenicol, erythromycin, novobiocin ...1976767065
threonine-sensitive homoserine dehydrogenase of serratia marcescens. 1975767250
epidemics of nosocomial urinary tract infection caused by multiply resistant gram-negative bacilli: epidemiology and control. 1976768384
the hydrolysis of nucleic acid by serratia marcescens. 1975768408
[the influence of thiamin on the physiology of the thiamin-autotrophic serratia marcescens (author's transl)]. 1975769416
[a method for cultivation of anaerobic microorganisms of manure samples (author's transl)]. 1975769417
perspectives of bacteremia and sepsis (lecture). 1976769660
postsurgical infections (lecture). 1976769661
antibiotic therapy in neonatal septicemia. 1976769663
in vitro studies of cefamandole.cefamandole is a new cephalosporin antibiotic that was tested in vitro against 540 clinical isolates of gram-positive cocci and gram-negative bacilli. a concentration of 0.39 mug/ml inhibited 95% of the isolates of staphylococcus aureus. a concentration of 6.25 mug/ml inhibited over 90% of the isolates of proteus mirabilis and escherichia coli, 69% of the isolates of klebsiella pneumoniae, and 31% of the isolates of indole-positive proteus spp. and enterobacter spp. it was active against most ce ...1976769679
a new method for measuring simultaneously the phagocytic and bactericidal capacity of human leukocytes.a new technique for simultaneously measuring the phagocytic and bactericidal capacity of human leukocytes is proposed. the method uses 14c labelled bacteria and is based on the principle that only viable intra-cellular bacteria incorporate 3h-thymidine. phagocytosis is measured by the ratio intra-cellular 14c/extra and intra-cellular 14c and the bactericidal capacity of leukocytes by the difference between the 3h-thymidine incorporation of the ingested and non-ingested bacteria. results in norma ...1975769857
functional hybrid enzymes reconstituted from escherichia coli and serratia marcescens rna polymerase subunits.rna polymerase was isolated from escherichia coli and serratia marcescens. the subunits of both enzymes were separated by electrophoresis on cellulose acetate sheets in the presence of urea. under conditions favouring reconstitution of the rna polymerases, stoichiometric amounts of the subunits were allowed to interact. active hybrid enzymes were formed if corresponding subunits of both enzymes were mutually exchanged. the analysis of the rna products synthesized showed that the reconstituted en ...1976770171
relationship of structure to function in bacterial endotoxins. ix. differences in the lipid moiety of endotoxic glycolipids.chemical, immunochemical, chromatographic, and endotoxic properties of five chromatographically pure glycolipids were compared. the preparations were extracted by chloroform-methanol from three escherichia coli, one salmonella minnesota, and one s. typhimurium re heptoseless mutant strains. the local shwartzman skin assay, the nonspecific resistance-enhancing effect, and the limulus assays could not distinguish among the five glycolipids, all five being active in all three assays. significant di ...1976770436
tryptophan operon regulation in interspecific hybrids of enteric bacteria.we examined tryptophan regulation in merodiploid hybrids in which a plasmid carrying the trp operon of escherichia was introduced into trp mutants of other enteric genera, or in which a plasmid carrying the trpr+ (repressor) gene of e. coli was transfered into fully constitutive trpr mutants of other genera. in these hybrids the trp operon of one species is controlled by the repressor of a different species. similar investigations were possible in transduction hybrids in which either the trp ope ...1976770450
medium-dependent inhibition of peptostreptococcus anaerobius by sodium polyanetholsulfonate in blood culture media.of 13 species of anaerobic cocci, peptostreptococcus anaerobius was the only species tested that was sensitive to 0.1% sodium polyanetholsulfonate (sps). however, the sensitivity of p. anaerobius to sps varied according to the media in which the cultures were grown. in supplemented peptone (b-d) and brain heart infusion media, most strain of p. anaerobius were not inhibited by sps. gelatin and proteose peptone were the medium components which were protective. the minimal inhibitory concentration ...1976770497
operative treatment of active endocarditis.during the past decade 44 patients with active endocarditis, defined as valvular infection requiring operative intervention before completion of a planned course of antibiotic therapy, have been treated at stanford university medical center. twenty-seven patients had infection of a native valve (primary endocarditis) and 17 had infection of a previously implanted intracardiac prosthesis. in 91 per cent of cases urgent valve replacement was dictated by rapid hemodynamic deterioration and in the r ...1976772323
[letter: serratia marcescens septicemia treated by nalidixic acid in perfusion]. 1976772599
intraventricular treatment of serratia marcescens meningitis with gentamicin. pharmacokinetic studies of gentamicin concentration in one case.a neurosurgical patient with postoperative meningitis caused by serratia marcescens was treated with intraventricular as well as intramuscular gentamicin. gentamicin concentration in serum and csf was determined at different times after the administration. serratia marcescens could not be isolated from csf after 3 days of therapy. determination of gentamicin concentrations in csf showed that gentamicin should not be given intraventricularly more than once a day to avoid accumulation of the drug. ...1976772794
the reversal of glucose repressed prodigiosin production in serratia marcescens by the cyclic 3'5'-adenosine monophosphate inhibitor theophylline.glucose was found to cause severe repression of prodigiosin production in serratia marcescens and a dose related partial reversal was demonstrated by theophylline. it is suggested that this reversal is due to the inhibition of camp phosphodiesterase and the concomitant increase in cellular camp concentration.1976773661
inhibition of prodigiosin formation in serratia marcescens by adenosine triphosphate.atp, inorganic phosphate and ribose inhibited prodigiosin formation in serratia marcescens, but adenine did not. atp was not hydrolyzed by the organism during the experiment.1976773662
[relation of acetoin and valine in enterobacteriaceae cultures on media with glucose and glycerin].the ratio between acetoin and valine produced by the cultures of the family enterobacteriaceae changes upon the addition of glucose and glycerol or their mixture to a defined medium. the experiments were carried out with 12 strains of aerobacter aerogenes and 2 strains of aerobacter cloacae and serratia marcescens. the highest yield of acetoin was found on the medium with glucose (12.5--13.0 g/litre); the maximum accumulation of valine was registered on the medium with glucose and glycerol (1.8- ...1975775256
[treatment of irradiated guinea pigs with the polysaccharide prodigiozan]. 1976775528
[serratia marcescens infections in chronic hemodialysis patients]. 1975775852
purification and properties of the alpha2beta2 complex of tryptophan synthetase of proteus mirabilis.a procedure is described for the purification of the tryptophan synthetase alpha2beta2 complex from cell extracts of proteus mirabilis. a 30-fold purification was achieved with an overall yield of about 23% and a specific activity of 1,600. the complex can be dissociated and the subunits isolated in a pure form. the complex can be reconstituted from the isolated subunits to regain the initial activity. the alpha and beta2 subunits of the tryptophan synthetase complex of p. mirabilis are not sign ...1976776977
relationship of structure to function in bacterial endotoxins: serologically cross-reactive components and their effect on protection of mice against some gram-negative infections.rabbit antisera were prepared against the heptoseless re mutants, salmonella minnesota r595 and s. typhimurium sli102, as well as against purified r595 glycolipid coated on autologous erythrocytes. the antisera cross-reacted with the endotoxic glycolipids extracted from re mutants of various bacterial strains, including s. minnesota r595, s. typhimurium sli102, escherichia coli d3im4, e. coli d2if2 and e. coli f515, as shown by passive haemagglutination and gel diffusion tests. the anti-re sera ...1976778329
a nursery outbreak caused by serratia marcescens--scalp-vein needles as a portal of entry.serratia marcescens rarely causes infections in newborn infants. we recently studied an epidemic caused by a multiply-resistant, serotype 014:h12 serratia marcescens that involved 42 infants. cutaneous abscesses at previous intravenous infusion sites occurred nine times, usually required surgical drainage, and were the most striking infections during the outbreak. six infants developed serratia bacteremia and two died with serratia meningitis; 34 patients were colonized with serratia but remaine ...1976778370
proceedings: on new biological responses of conventional adult mice administrated with bacterial endotoxin. 1975778449
[resistance to beta-lactam antibiotics and aminoglycosides in gram negative bacteria. 1. molecular and genetic characterization of r-factors (author's transl)].with frequent use of aminoglycoside antimicrobials and beta-lactam antibiotics in hospitals in the last few years, the number of bacterial strains resistant to these chemotherapeutics increased. lately, strains of e. coli, klebsiella, enterobacter, serratia, proteus and pseudomonas resistant to many antimicrobials (ampicillin, carbenicillin, cephalothin, chloramphenicol, gentamycin, tobramycin, sisomycin, neomycin, paromomycin, kanamycin, streptomycin, spectinomycin, tetracycline, sulphonamides) ...1976779353
[resistance to beta-lactam antibiotics and aminoglycosides in gram negative bacteria. 2. mechanism of resistance (author's transl)].in a preceding paper the genetics of resistance of 2 representative strains exhibiting resistance to beta-lactam antibiotics (ampicillin, catbenicillin, cephalothin) to aminoglycosides (kanamycin, neomycin, paromomycin, gentamycin, sisomycin, tobramycin, streptomycin, spectinomycin) and further antimicrobials (tetracycline, chloramphenicol, suphonamides) were described. this paper reports about the mechanism of resistance to beta-lactam antibiotics and aminoglycosides in these strains. enzymatic ...1976779354
studies on group a (phage tail) bacteriocins of serratia marcescens. v. serological characterization of subgroup i and ii bacteriocins.neutralization tests with rabbit hyperimmune sera revealed a close, if not identical, serological relationship among 7 group a (phage tail) bacteriocins of serratia marcescens of subgroup i, and among 3 phage tail bacteriocins of subgroup ii, respectively. on the other hand, subgroup i and ii phage tail bacteriocins were found to be serologically unrelated, as determined with neutralization tests and ouchterlony immunodiffusion experiments. immunoelectrophoretic tests, employing a representive p ...1976779357
studies on group a (phage tail) bacteriocins of serratia marcescens. vi. calcium ion-dependent biological activity of subgroup ii bacteriocins.the biological activity of subgroup ii group a (phage tail) bacteriocins of serratia marcescens against susceptible indicator cells was completely abolished on two defined, agarose-containing media. the addition of 0.002 m cac12 to these two media fully restored the lethal activity of these phage tails. subgroup i phage tail bacteriocins, on the other hand, were found to have no requirement for divalent cations. these observations furnished an additional biological criterion for the differentiat ...1976779358
comparison of methods for differentiating among serratia marcescens isolated from clinical specimens.serotyping, biotyping and antibiotic resistance patterns were found to give consistent results for routine hospital surveillance of serratia marcescens isolates. bacteriocin typing showed variation between trials and was considered too variable and time-consuming for routine hospital surveillance.1976779447
[urinary infections due to serratia marcescens: clinico-statistical pictures of patients hospitalized in the s. maria della scala hospital in siena during 1971, 1972, and 1973].the authors have carried out a clinical-statistical research into cases of urinary tract infection by serratia marcescens isolated in 42 of 2500 urine-cultures effected during the period 1971-1973 by patients hospitalized in "s. maria della scala" hospital of siena. the authors point out the high incidence of isolation of serratia marcescens in urological patients and show some control measures to prevent the diffusion of such infections in hospital.1975779676
combined serotyping and biotyping of serratia marcescens.the api (analytab products, inc., new york, n.y.) biotypes of 117 clinical isolates of serratia marcescens were determined and fell into 13 different patterns. the o and h antigens were determined by tube agglutination, and 27 serotypes were identified. the biotype and serotype appeared to vary indepently. serotyping and biotyping combined divided these isolates into 56 different types. there was a problem interpreting the end points for inositol fermentation and urease production, which could a ...1976780372
correlation between generation times and l-threonine dehydratase activities in isoleucine revertants of serratia marcescens. 1976781184
[formation of l-asparagine and l-glutamine deamidases by bacterial cultures].among studied 40 bacterial cultures, 17 strains catalysed hydroxylaminolysis of i.-asparagine and l-glutamine, and among these cultures seven strains belonged to the pseudomonas genus. extracts of the cells of ps. boreopolis 526 (mgu), ps. aurantiaca ibfm b-14, and ps. septica ibfm b-40 had the maximum deamidase activity during the stage of decelerated growth. the activity of l-asparaginase 2 was practically the same upon various ways of disintegration of the cells of ps. aurantiaca ibfm b-14: b ...1976781474
serum amylase in rats following the administration of endotoxin. 1976781914
sensitivity of hospital strains of serratia marcescens to aminoglycosides. 1976782126
macromolecular syntheses during biosynthesis of prodigiosin by serratia marcescens.amino acids that were utilized as sole sources of carbon and nitrogen for growth of serratia marcescens nima resulted in biosynthesis of prodigiosin in non-proliferating bacteria. addition of alanine, proline, or histidine to non-proliferating cells incubated at 27 c increased the rate of protein synthesis and also caused biosynthesis of prodigiosin. no increase in the rate of protein synthesis was observed upon the addition of amino acids that did not stimulate prodigiosin biosynthesis. increas ...1976782359
[induction of protease synthesis in serratia marcescens]. 1976782568
[sisomicin versus gentamicin. a comparison of antibacterial and pharmacokinetic properties (author's transl)].in a comparison of the antibacterial in-vitro activity of sisomicin, gentamicin, and tobramycine, sisomicin showed a higher activity against e. coli, indole-positive proteus spp. and organisms of the klebsiella-enterobacter-serratia group, whereas tobramycine was superior against pseudomonas spp. however, the differences in activity between sisomicin and gentamicin were only within one step of dilution which is hardly sufficient to guarantee a basic superiority of one or the other preparation fo ...1976782808
l-norvaline and l-homoisoleucine formation by serratia marcescens,.two unnatural amino acids were found as by-products in isoleucine fermentation from threonine by serratia marcescens. these amino acids were identified as l-norvaline (2-aminopentanoic acid) and l-homoisoleucine (2-amino-4-methylhexanoic acid). formation of l-norvaline and l-homoisoleucine was not observed when l-leucine was added to the medium. the leucine auxotroph derived from the isoleucine accumlator did not produce l-norvaline or l-homoisoleucine even during the accumulation of large amoun ...1976783153
[on clinical observations of urinary tract infections by serratia marcescens (author's transl)]. 1976785067
letter: serratia marcescens endocarditis. 1976786096
purification, subunit structure and partial amino-acid sequence of anthranilate-5-phosphoribosylpyrophosphate phosphoribosyltransferase from the enteric bacterium serratia marcescens.the enzyme anthranilate-5-phosphoribosylpyrophosphate phosphoribosyltransferase from serratia marcescens was purified to apparent homogeneity. the purification procedure included ammonium sulfate precipitation, deae-cellulose chromatography, sephadex gel filtration and hydroxyapatite chromatography. the molecular weight of the native protein as determined on a calibrated sephadex g-200 column was 45000. dodecylsulfate-polyacrylamide gel electrophoresis in the presence of reducing agent revealed ...1976786623
formation of crystalline deposits by several genera of the family enterobacteriaceae.several species of bacteria from the family enterobacteriaceae formed crystalline materials containing calcium when grown in a defined culture medium. enterobacter aerogenes, proteus vulgaris, citrobacter freundii, and c. intermedius produced calcium pyrophosphate crystals. edwardsiella tarda and escherichia coli formed calcite iii crystals, whereas proteus mirabilis, klebsiella pneumoniae, providencia stuartii, and serratia marcescens produced hydroxyapatite crystals. several of these bacteria ...1976786887
an outbreak of nosocomial infection due to multiply resistant serratia marcescens: evidence of interhospital spread.interhospital spread appeared to be responsible for a large epidemic of infections due to a strain of serratia marcescens that was resistant to all currently available parenteral antibiotics. between april 1, 1973 and january 1, 1975, 210 patients in four geographically separate hospitals in nashville, tennessee, were infected with the epidemic strain; 21 patients were bacteremic and eight died. catheter-associated urinary tract infection accounted for the majority of isolates, and broad-spectru ...1976787446
study on pathogenicity, drug sensitivity and pigment production of serratia marcescens. 1976787472
cleavage of lambda dna by a site-specific endonuclease from serratia marcescens.three sites recognized by smai endonuclease, purified from serratia marcescens sb, have been located on lambda dna at 0.406, 0.656, and 0.825 fractional lengths from the left end of the dna molecule.1976787556
[serratia marcescens infection in intensive care units (author's transl)].the increased incidence of serratia marcescens infection at the intensive care unit of the department of anaesthetics, general hospital altona, was investigated. the properties of this microorganism are described and its role in cross infections is discussed. the frequent occurrence of serratia marcescens in mixed infections and its tendency to grow on tissue surfaces are pointed out. the presence of serratia marcescens was demonstrated in 23 of 107 patients treated in the intensive care unit; a ...1975787963
tobramycin: a review of its antibacterial and pharmacokinetic properties and therapeutic use.synopsis: tobramycin is a new aminoglycoside antibiotic with a broad antibacterial spectrum in vitro, and pharmacokinetic properties similar to those for gentamicin. tobramycin is more active than gentamicin against pseudomonas aeruginosa and active against many gentamicin resistant strains, but is not active against enterobacteriaceae resistant to gentamicin. theoretically, tobramycin has an advantage over gentamicin against infections caused by p. aeruginosa, but any advantage in clinical prac ...1976789045
[antimicrobial effectiveness of sisomicin. i: in vitro activity of sisomicin compared with gentamicin, tobramycin, amikacin and kanamycin (author's transl)].the aminoglycosides sisomicin, gentamicin, tobramycin, amikacin and kanamycin are highly active against staphylococci including the penicillinase-positive strains. sisomicin is more effective than amikacin and kanamycin. mixed infections with staphylococci and enterobacteriaceae or pseudomonas aeruginosa are thus on indication for treatment with sisomicin or other aminoglycosides. infections with e. coli, enterobacter, susceptible klebsiella, and susceptible pseudomonas strains can be treated wi ...1976789246
serratia arthritis. report of seven cases. 1976789379
beta-lactamases and resistance to penicillins and cephalosporins in serratia marcescens.strains of serratia marcescens fall into one of two groups with respect to their resistance to to beta-lactum antibiotics. most strains are highly resistant to cephalosporins but are significantly more susceptible to ampicillin and carbenicillin, whereas other strains are highly resistant to both penicillins and cephalosporins. strains in the former category produce small amounts of an inducible cephalosporinase, which appears to be chromosomally mediated. strains in the latter class also elabo ...1976789784
specific and nonspecific immunity to serratia marcescens specific active or passive immunization, mice were protected against lethal infection with serratia marcescens. animals that were immunized against organisms of the challenge strain o serotype survived, whereas animals that were immunized against other serratia o serotypes did not survive. protective sera (from convalescent partients or immunized rabbits) contained a specific complement-independent opsonin. these sera also contained passive hemagglutinating and bacterial agglutinating antibod ...1976789788
serratia marcescens and the urologist.serratia marcescens, long considered a non-pathogen, is now found to be responsible for outbreaks of nosocomial infections. an outbreak of serratia infection at 2 institutions is reported, in which 253 cultures of serratia were grown and 115 patients were involved. the 3 most important conditions that preceded isolation of serratia were the use of indwelling urethral catheters, antibiotic therapy and operation. all infections were acquired in the hospital. an epidemiological survey showed that t ...1976789918
[control of cross infection in intensive care units].quantitative determinations of antibiotic susceptibility established that infections occurring in intensive care units were predominantly exogenous cross infections. the most frequently isolated strains were: klebsiella, enterobacteriaceae, serratia marcescens and proteus mirabilis. the majority of the strains showed multiple resistance. despite adequate antibiotic therapy a number of patients died of the infection. many of the methods of disinfection were found to be inadequate, especially as r ...1976790370
[disinfection of the inspired and expired air during artificial respiration with humified gases].the water in the nebulizer of respirators is a potential source of infection for patients receiving artificial respiration. interposition of a special equipment that will produce ozone-free ultraviolet radiation will prevent infection of the patient by the inspired humidified air, even if the bacterial count in the nebulizer fluid is very high. interposition of the same equipment on the expiratory side is also recommended to minimize the risk of infection of the environment by bacteria in the ex ...1976790371
[the biological significance of various cell-wall preparations of gram negative bacteria (proceedings)]. 1976790849
r factor-mediated antibiotic resistance in serratia marcescens.nineteen of 39 multiresistant strains of serratia marcescens isolated from clinical sources transferred antibiotic resistance to escherichia coli or klebsiella pneumoniae recipients. marcesins and/or phage prevented effective resistance transfer to e. coli and attempts to select marcescin-resistant mutants of the e. coli recipient strain were unsuccessful. transfer of resistance was demonstrated for all drugs tested except nalidixic acid. approximately 90% of donors resistant to tobramycin, ampi ...1976791085
role of l-proline in the biosynthesis of prodigiosin.nonproliferating cells of serratia marcescens, wild-type strain nima, synthesized the pigment, prodigiosin, when saline suspensions were incubated with aeration at 27 degrees c in the presence of proline or alanine. mutants puts1 and puts2 derived from strain nima formed prodigiosin from alanine, but not from proline, unless alanine also was added. strain nima utilized proline as a sole source of carbon and of nitrogen for growth, whereas put mutants did not. investigation of enzymes degrading p ...1976791123
serratia marcescens - caused arthritis with negative and positive birefrengent crystals.we encountered an unusual case of arthritis caused by serratia marcescens, with both positive and negative birefringent crystals in the same inflammatory synovial fluid. this combination of events is most likely to occur in men over 40 years old who have a predisposing illness or are receiving immunosuppressive drugs. this case shows the need to consider multiple pathological processes occurring in the same joint.1976791169
clinical evaluation of the minitek differential system for identification of enterobacteriaceae.forty-one stock organisms and 581 fresh clinical isolates were used in comparing the minitek system to conventional tubed media to determine if this system is feasible and accurate for a high-volume clinical microbiology laboratory. in addition to comparison with tubed media, the following parameters were tested: (i) reproducibility of disks, (ii) the effect of variation in inoculum size, (iii) the effect of the age of culture, and (iv) the effect of predispensing disks. a total of 5,947 disks w ...1976791964
[neonatal serratia marcescens infections]. 1976793738
biosynthesis of norvaline, norleucine, and homoisoleucine in serratia marcescens.the biosynthetic pathways of norvaline homoisoleucine were examined using regulatory mutants of leucine biosynthesis in serratia marcescens. alpha-isopropylmalate synthetase [ec], the first enzyme of leucine biosynthesis, catalyzed the condensations of acetyl-coa with pyruvate, alpha-ketobutyrate, alpha-ketovalerate, or alpha-keto-beta-methylvalerate as well as alpha-ketoisovalerate. these condensations were inhibited by leucine in the alpha-aminobutyrate-resistant mutant, a mutant with ...1976794063
genetic studies of the ribosomal proteins in escherichia coli. ix. mapping of the ribosomal proteins, s2 and s20, by intergeneric mating experiments between serratia marcescens and escherichia coli k12.episomes of e. coli k12, which cover thrleu region of the chromosome, were transferred to serratia marcescens. ribosomal proteins from these hybrid strains were analyzed with phosphocellulose column chromatography. two e. coli 30s ribosomal proteins, s2 and s20, could be detected in the ribosome of the hybrid strain in addition to all ribosomal proteins of s. marcescens.1976794688
the extracellular metalloprotease of serratia marcescens. 2. comparison with trypsin and substrate specificity.the proteolytic activity of the extracellular protease of serratia marcescens was compared with that of trypsin on n, n-dimethyl casein. the peptides produced from exhaustive hydrolysis of alpha casein by the protease and by trypsin were of similar size as measured by gel filtration on p-10 agarose. we conclude that the protease of s. marcescens in an endopeptidase with trypsin-like activity on proteins, producing oligopeptides. end group analysis of the peptides formed by the s. marcescens prot ...1976794698
in vitro activity of gentamicin, amikacin and netilmicin alone and in combination with carbenicillin against serratia marcescens.the inhibitory and bactericidal effects of gentamicin, amikacin, netilmicin (sch 20569), and carbenicillin were tested against 55 clinical isolates of serratia marcescens that had been subtyped into 26 strains by biotyping and serotyping. three major patterns of resistance to gentamicin, netilmicin, and carbenicillin were recognized among these isolates. (i) most of the 27 isolates that were susceptible to gentamicin (minimal bactericidal concentration [mbc] </=6.25 mug/ml) were susceptible to c ...1976795373
[physical mapping of products of phage lambda dna hydrolysis by serratia marcescens restriction endonuclease]. 1976795624
[dynamics of accumulation of extracellular proteins of serratia marcescens and their nuclease activity during cell growth].serratia marcescens, strain b-10 m-1, liberates an unspecific endonuclease into the extracellular nutrient solution. two peaks of the enzyme activity were found in the cultural broth during growth of the cells. the dynamics of accumulation of protein fractions in the cultural broth was studied, and the relative electrophoretic mobility of the enzyme-active part of the proteins was established.1976796632
genetic studies of the ribosomal proteins in escherichia coli. x. mapping of the ribosomal proteins, l21 and s15, by intergeneric mating experiments between serratia marcescens and escherichia coli k12.episomes of e. coli, which cover argg but not the str region, were transferred to serratia marcescens. ribosomal proteins from these hybrid strains were analyzed with phospho-cellulose or carboxy-methyl-cellulose column chromatography. two e. coli ribosomal proteins, l21 and s15, could be detected in the ribosome from the hybrid strains in addition to the ribosomal proteins of s. marcescens.1976796677
[intracellular organisation of bacteriophage tail-like bacteriocins of group a in serratia marcescens (author's transl)].the biosynthesis of a phage tail-like bacteriocin by cells of the group a-bacteriocinogenic (ba+) serratia marcescens strain no. 16 after induction with mitomycin c (mc) was examined electron-microscopically. this bacteriocin (total length 117 nm) consists of a hollow core and a contractile sheath. at 60 min following induction, rod-like bacteriocin-particles were identifiable in ultrathin sections. the particles were found to comprise three morphologically different forms of aggregation: 1. hex ...1976797334
endotoxin-induced non-specific resistance in rats examined by trypanosoma equiperdum rats weighing 100 g, intravenously administered trypanosoma equiperdum organisms started logarithmic growth immediately, whereas in rats weighing 150-300 g growth started after a lag phase of 0.7-3.1 hr. the lag phase lasted for 15-17 hr when the rats were pretreated serially with endotoxins and this time course was not modified by changes either in the host's body weight or in the germ count of the inocula. maximum resistance to infection was achieved with gradual doses of the endotoxin give ...1976798472
infection in the intensive care epidemic of infection associated with serratia marcescens and other gram-negative organisms resistant to aminoglycosides and other chemotherapeutic agents occurred in the intensive care unit, and spread to other areas of the hospital. this paper describes the problems of sepsis in the critically ill patient, outlines the occurrence of organisms in the patients concerned in this epidemic, and discusses the policies adopted to control the incidence of life-threatening infection caused by bacter ...1976799504
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