Publications

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adhesion of probiotic lactobacilli to chick intestinal mucus.in the present work, interactions between three lactobacillus strains (lactobacillus fermentum crl1015, lactobacillus animalis crl1014, and lactobacillus fermentum crl1016) and chicken small intestinal mucus were determined. three lactobacilli isolated from chicken and selected by their potentially probiotic properties were able to grow in mucus preparations. three peaks from gel filtration chromatography of intestinal mucus were obtained. the adhesion to three mucus fractions (i, ii, and iii), ...200314569288
on the desoxycholatesensitive strains of salmonella pullorum. 196014497432
[variations in carbohydrate metabolism by salmonella gallinarum and salmonella pullorum; their diagnostic value]. 195414350347
biochemical differentiation of salmonella gallinarum and salmonella pullorum cultures. 196514334406
the control of pullorum disease in taiwan. 196514298862
isolation of salmonella pullorum from a japanese pheasant. 196514284966
is eradication of pullorum disease realistic? 196414118007
the differentiation of salmonella pullorum and salmonella gallinarum by biochemical methods. 196213993927
[on the nutrition of salmonella pullorum and salmonella gallinarum. 3. on the purine requirement of salmonella pullorum strain no. 21]. 196213938795
[on the nutrition of salmonella pullorum and s. gallinarum. 2. comparison of the growth-supporting ability of various synthetic media and the improvement of yasushi's medium]. 196213938794
the effect of methyl bromide upon salmonella pullorum. 196213932003
[studies on the salmonellosis of fowls with special reference to diagnosis of the s. pullorum carrier. ii. studies on the "intermittent type" of the pullorum disease in artificially infected fowls]. 196113919500
naturally occurring variants or salmonella pullorum possessing an unusual sensitivity to bile salts and other agents. 196213914263
effect of the administration of cortisone on the response of chicks to the endotoxin of salmonella pullorum. 195913817600
the association of virulence with endotoxin in salmonella pullorum. 196013807157
[studies on the bactericidal action of the incubated egg, with special reference to salmonella pullorum and s. senftenberg. 1. examination of experimental conditions]. 196113712119
[studies on the bactericidal action of embryonated eggs against salmonella pullorum and s. senftenberg. 2. the bactericidal action of humours in embryonated eggs]. 196113712118
[conditioned hemagglutination and antiglobulin tests in the detection of complete and incomplete antibodies in salmonella pullorum infections]. 195813603050
glycogen reduction in chick liver during pullorum disease. 195813563421
growth response of salmonella pullorum in the chicken embryo. 195813559631
[investigation on antigenic value of isolated in bulgaria strains of salmonella gallinarum (syn. salmonella pullorum) in diagnosis of pullorosis before incubation]. 195813549099
[a variant of salmonella pullorum]. 195713522255
furazolidone treatment of experimental pullorum disease in adult chickens. 195813498261
influence of salmonella pullorum infection on various liver tricarboxylic acid enzymes and citrate levels in the chick. 195613376507
modification of pullorum disease in the chick by certain tricarboxylic acid cycle inhibitors and intermediates. 195613376506
[invalidness of the distinction between salmonella gallinarum and salmonella pullorum and their mutual biochemical variants]. 195613340328
alterations in the nitrogen excretion pattern of chicks infected with salmonella pullorum. 195613331863
probit analysis applied to salmonella pullorum survival data. 195613295196
the effect of the introduction of amino acids into chicks infected with salmonella pullorum. 195513263285
the effect of salmonella pullorum infection on amino acids of the chick. 195513263284
a simplified method for typing salmonella pullorum cultures. 195513242469
the relative importance of body temperature and lymphocytes in genetic resistance to salmonella pullorum in the fowl. 195513238751
a comparison of six enrichment media for isolating salmonella pullorum from egg-products. 195313105290
acute ulcerative colitis successfully treated with chloramphenicol; report of case due to salmonella pullorum. 195313096327
synthesis of glutamic acid and alanine by salmonella pullorum. 195313084550
isolation of salmonella pullorum from a chinchilla. 195313084489
antigenic studies using ammonium sulfate. ii. the macroscopic ammonium sulfate sedimentation test for distinguishing the antigenic forms of salmonella pullorum. 195313065663
antigenic studies using ammonium sulfate. i. the relative sedimentation effect of ammonium sulfate on the various antigenic types of salmonella pullorum. 195313065662
observations on the cobalt enhancement of penicillin activity against salmonella pullorum. 195313056582
growth curves of salmonella pullorum in different media. 195313022632
hematology, plasma biochemistry, and serosurvey for selected infectious agents in southern giant petrels from patagonia, argentina.in conjunction with reproductive and feeding ecology studies on southern giant petrels (sgp, macronectes giganteus) blood samples were collected for baseline health evaluations. twenty-five adult sgp from a breeding colony in chubut, argentina, were sampled during two consecutive breeding seasons, 1999-2000 (n = 15) and 2000-01 (n = 10). values for hematology, plasma biochemistry, and minerals are described for 20 birds in apparent good physical condition. a serologic survey of exposure to selec ...200312910764
effect of dietary formic and propionic acids on salmonella pullorum shedding and mortality in layer chicks after experimental infection.this investigation was conducted to study the effect of dietary formic acid (fa) and propionic acid (pa) mixture on inhibitory effect of salmonella pullorum in layer chicks. nine equal groups of 1-day-old layer chicks, in addition to positive and negative controls, were fed on day 3 of age with acid-treated feed containing mixture of both acids at concentrations, from 0.5 to 1.5%. positive and negative controls were fed untreated feed. all groups except the negative control were challenged orall ...200312667187
salmonella enterica serovar pullorum requires the salmonella pathogenicity island 2 type iii secretion system for virulence and carriage in the chicken.functional mutations were made in the type iii secretion systems encoded by salmonella pathogenicity island 1 (spi 1) and salmonella pathogenicity island 2 (spi 2) of salmonella enterica serovar pullorum, the cause of pullorum disease in poultry. their role in cell invasion in vitro, and in virulence in vivo was determined. the spi 1 mutant showed decreased invasiveness for chicken cells but was capable of causing disease in orally infected 1-day-old chicks, although it showed some reduction in ...200212427344
improvements required for the detection of salmonella pullorum and gallinarum.detection of the specific salmonella serovar gallinarum, which is divided into the biovars pullorum and gallinarum, is compulsory under the national hygienic and sanitary control regulations of france for breeding flocks whose offspring are exported. our aim was to examine the suitability of bacteriologic and serologic methods routinely used in france to screen serum samples and organs for s. gallinarum. since bacteriologic reference techniques are designed to isolate the commonly occurring non- ...200212146886
surface plasmon resonance (biacore) detection of serum antibodies against salmonella enteritidis and salmonella typhimurium.we have used a surface plasmon resonance biosensor (biacore 3000) to detect serum antibodies in chickens having current or recent infections. three well-defined salmonella flagellar recombinant dna antigens reflecting salmonella enteritidis (h:g,m flagellin) and salmonella typhimurium (h:i and h:1,2 flagellins) expressed in escherichia coli were each immobilized in a single flow cell of a biosensor chip. glutathione-s-transferase was immobilized on the surface of another flow cell to monitor non ...200212133620
the evolving genome of salmonella enterica serovar pullorum.salmonella enterica serovar pullorum is a fowl-adapted bacterial pathogen that causes dysentery (pullorum disease). host adaptation and special pathogenesis make s. enterica serovar pullorum an exceptionally good system for studies of bacterial evolution and speciation, especially regarding pathogen-host interactions and the acquisition of pathogenicity. we constructed a genome map of s. enterica serovar pullorum rks5078, using i-ceui, xbai, avrii, and spei and tn10 insertions. pulsed-field gel ...200211976291
pathogenicity of different serogroups of avian salmonellae in specific-pathogen-free chickens.the pathogenicity of one isolate of salmonella typhimurium, four isolates of salmonella heidelberg, three isolates of salmonella kentucky, two isolates of salmonella montevideo, one isolate of salmonella hadar, and two isolates of salmonella enteritidis (se), one belonging to phage type (pt)13a and the other to pt34, was investigated in specific-pathogen-free chicks. three hundred eighty-four chicks were separated into 16 equal groups of 24 chicks. thirteen groups were inoculated individually wi ...200111785896
classification of grossly detectable abnormalities and conditions seen at postmortem in canadian poultry abattoirs according to a hazard identification decision tree.this study was designed to review all grossly detectable abnormalities and conditions (gdacs) encountered in poultry in canadian abattoirs to determine which have potential to cause adverse health effects for the consumer. review of the literature and consultation with scientists in the field of microbiology, epidemiology, poultry pathology, chemistry, and meat inspection served to generate an inventory of gdacs, and a decision tree containing algorithms was developed to identify gdacs potential ...200111770626
pathogenicity of environmental origin salmonellas in specific pathogen-free chicks.two hundred sixty 1-d-old specific pathogen-free (spf), single comb white leghorn chicks were used in this study to determine pathology caused by salmonella enteritidis (se) isolated from a poultry environment. the chicks were subdivided into 10 equal groups of 26 chicks each. eight groups of chicks were inoculated individually with 0.5 ml of brain heart infusion broth culture containing 1 x 10(6) cfu of se phage type (pt) -8 (1, 2, 3), se pt5 a (1, 2), or se pt4 (ch-env-ca, chicken-ca, and huma ...200111558918
loss of virulence by heterophil-adapted salmonella pullorum.we report that reduction of virulence for day-old chicks was achieved after eight-times-repeated heterophil passage of salmonella pullorum (sp). the virulent source strain sp-v caused 64% mortality and 89% internal organ as well as 89% cecal colonization 10 days after administration of 10(7) colony-forming units (cfu) to day-old chicks. eight-times-repeated passage of sp in heterophils resulted in attenuated strain sp-a that was nonlethal and reduced colonization of internal organs from 89% for ...200111417827
effect of different complex carbon sources on growth and bacteriocin synthesis of enterococcus faecium.different criteria are followed in order to select bacteria to be used in probiotic and symbiotic supplements. a new parameter to choose strains could be fermentation by intestinal bacteria of some complex carbohydrates because they are prebiotics and promote the development of beneficial microorganisms in the intestinal environment. an enterococcus faecium strain, isolated from the crop of a free-range chicken, was assayed in order to determine the utilization of commercial sugars and/or crude ...200111246907
serologic survey of slaughter-age ostriches (struthio camelus) for antibodies to selected avian pathogens.serum samples from 163 slaughter-age ostriches (struthio camelus) in ohio and indiana were tested for antibodies to avian influenza virus (aiv), newcastle disease virus (ndv), paramyxovirus (pmv) 2, pmv3, pmv7, infectious bursal disease virus (ibdv), bordetella avium, mycoplasma synoviae, mycoplasma gallisepticum, ornithobacterium rhinotracheale, salmonella pullorum, salmonella gallinarum, and salmonella typhimurium. one ostrich had antibodies to aiv h5n9, 57% of the ostriches had antibodies to ...200011195659
health status of northern bobwhite quail (colinus virginianus) in eastern kansas.the health status of wild northern bobwhite quail (colinus virginianus) from lyon county, kansas, was evaluated by conducting comprehensive health assessments on 25 birds. gross lesions indicative of avian pox, ulcerative enteritis, and quail bronchitis were not present. serologic tests for antibodies to salmonella pullorum, salmonella gallinarum, pasteurella multocida, mycoplasma gallisepticum, mycoplasma synoviae, and avian adenoviruses were all negative. intestinal coccidia (eimeria spp.) wer ...200011195653
new bisabolane sesquiterpenes from ligularia songarica.phytochemical investigation of ligularia songarica (compositae) afforded seven new bisabolane-type sesquiterpenes. their structures were confirmed on the basis of spectroscopic methods, especially 2d-nmr techniques, and compound 7 showed stronger antibacterial activity against escherichia coli, pseudomonas acruginosa and salmonella pullorum.200011189875
differential cytokine expression in avian cells in response to invasion by salmonella typhimurium, salmonella enteritidis and salmonella gallinarum.salmonella enterica is a facultative intracellular pathogen that is capable of causing disease in a range of hosts. although human salmonellosis is frequently associated with consumption of contaminated poultry and eggs, and the serotypes salmonella gallinarum and salmonella pullorum are important world-wide pathogens of poultry, little is understood of the mechanisms of pathogenesis of salmonella in the chicken. type iii secretion systems play a key role in host cell invasiveness and trigger th ...200011101679
protective effect of enterococcus faecium j96, a potential probiotic strain, on chicks infected with salmonella pullorum.enterococcus faecium j96 was isolated from a healthy free-range chicken and it inhibited salmonella pullorum, in vitro, due to its lactic acid and bacteriocin production. in vivo assays were carried out with 30-h-old broiler chicks. the lactic acid bacteria (approximately 1 x 10(9) cells per chick) were orally administered as preventive and as therapeutic treatments. in the first case they were given to the chicks twice a day for 3 consecutive days. in the second case the lactic bacteria were ad ...200011041131
fowl typhoid and pullorum disease.fowl typhoid (ft) and pullorum disease (pd) are septicaemic diseases, primarily of chickens and turkeys, caused by gram negative bacteria, salmonella gallinarum and s. pullorum, respectively. clinical signs in chicks and poults include anorexia, diarrhoea, dehydration, weakness and high mortality. in mature fowl, ft and pd are manifested by decreased egg production, fertility, hatchability and anorexia, and increased mortality. gross and microscopic lesions due to ft and pd in chicks and poults ...200010935271
characterization of swimming motility and identification of flagellar proteins in salmonella pullorum isolates.to identify swimming motility in salmonella pullorum isolates and to characterize the flagellar proteins produced by motile isolates.199910791949
differentiation of salmonella enterica serotype gallinarum biotype pullorum from biotype gallinarum by analysis of phase 1 flagellin c gene (flic).salmonella enterica serotype gallinarum biotype gallinarum and biotype pullorum are non-motile and pathogenic avian strains. biotype gallinarum causes fowl typhoid and biotype pullorum is the cause of pullorum disease in chickens. the two biotypes could be differentiated based on biochemical characteristics. however, conventional culture and biochemical assays are time-consuming, laborious and need sterile laboratory practices. although the two biotypes, gallinarum and pullorum are non-motile, t ...200010739340
the effects of dietary mannaoligosaccharides on cecal parameters and the concentrations of enteric bacteria in the ceca of salmonella-challenged broiler chicks.the ability of different enteric pathogens and coliforms to trigger agglutination of yeast cells (saccharomyces cerevisiae, ncyc 1026) and a yeast cell wall preparation (mos) was examined. five of seven strains of escherichia coli and 7 of 10 strains of salmonella typhimurium and salmonella enteritidis agglutinated mos and sac. cerevisiae cells. strains of salmonella choleraesuis, salmonella pullorum, and campylobacter did not lead to agglutination. two strains that agglutinated mos (s. typhimur ...200010735748
some probiotic properties of chicken lactobacilli.the beneficial effect of lactobacilli has been attributed to their ability to colonize human and animal gastrointestinal tracts. in this work, adhesion assays with three lactobacillus strains and intestinal fragments obtained from chickens were assessed. lactobacillus animalis and l. fermentum were able to adhere to three kinds of epithelial cells (crop, small and large intestines) with predominance to small intestine. among the strains considered, l. fermentum subsp. cellobiosus showed the lowe ...199910696476
antibodies to selected disease agents in translocated wild turkeys in california.wild turkeys (meleagris gallopavo) trapped within california (n = 715) or imported into california from other states (n = 381) from 1986 to 1996 were tested for exposure to certain disease agents. prevalence of antibody to mycoplasma gallisepticum, mycoplasma meleagridis, salmonella pullorum, salmonella typhimurium, newcastle disease virus, and avian influenza virus was low (0-4%) for wild turkeys trapped within california. with the exception of antibody prevalence to m. meleagridis of 33%, the ...200010682760
temporal changes in the population genetics of salmonella pullorum.salmonella pullorum is the cause of pullorum disease, which is characterized by white diarrhea and a high mortality rate in poultry. during the 1990s, the serologic "pullorum" test has occasionally failed to detect infected birds during the early stage of disease. to determine if any recent genetic changes have taken place in s. pullorum to account for poor seroconversion sometimes observed in infected flocks, s. pullorum from 1990s outbreaks and strains isolated prior to the 1980s were typed by ...199910611985
salmonella typhimurium infection in domesticated fowl in a children's zoo.salmonella typhimurium infection occurred in a children's zoo where 11 fowl and 85 mammals were kept. initially, the guinea pigs were infected and transmitted the infection to the fowl and rabbits. these mammals responded to medication and cleared of the infection; however, the birds were judged to contain excreters despite four regimens of treatment with antibiotics. cloacal swabs were taken from all the birds. one turkey was positive for salmonella and was destroyed. pooled fecal samples from ...199910494436
pathogenicity of salmonella enteritidis phage types 4, 8, and 23 in broiler chicks.four hundred fifty day-old hubbard broiler chicks were subdivided into 15 groups of 30 chicks each. six groups of chicks received 0.5 ml of broth culture containing 5 x 10(6) colony-forming units (cfu) of salmonella enteritidis (se) phage types (pts) 4, 8, and 23 by crop gavage. similarly, six other groups received 0.5 ml containing 5 x 10(8) cfu of se. one group was inoculated with 0.5 ml containing 5 x 10(6) cfu of salmonella pullorum, and another group received 0.5 ml containing 5 x 10(8) cfu ...199910494420
vaccination for control of salmonella in poultry.salmonella spp. are facultative intracellular pathogens causing localised or systemic infections, in addition to a chronic asymptomatic carrier state. they are of worldwide economic and public health significance. in poultry, which represent important sources of cheap protein throughout the world, fowl typhoid and pullorum disease continue to cause economic losses in those parts of the world where the poultry industries are continuing to intensify and where open sided housing is common. a number ...199910418900
early events in the pathogenesis of avian salmonellosis.salmonellae are gastrointestinal pathogens of man and animals. however, strains that are host-specific avian pathogens are often avirulent in mammals, and those which are nonspecific are commensal in poultry. the objective of this study was to determine whether host specificity was exhibited by bacterial abilities to invade epithelial cells or resist leukocyte killing. in this study, leukocytes isolated from humans and chickens were used to kill salmonella in vitro. both salmonella pullorum, an ...199910377142
health evaluation of free-ranging rockhopper penguins (eudyptes chrysocomes) in argentina.as part of annual colony counts in santa cruz province, argentina, a health survey of rockhopper penguins (eudyptes chrysocomes) was conducted in 1994. forty-five birds were examined during handling procedures, and blood and fecal samples were collected for laboratory analysis. all birds appeared to be in good condition. no ecto- or endoparasites were found. hematology, plasma chemistry, and plasma mineral levels were measured and correlated with the results of bacterial and viral serology. anti ...199910367640
salmonella pullorum in the common pheasant (phasianus colchicus).in 1996, pullorum disease due to salmonella enterica serovar gallinarum biovar pullorum (salmonella pullorum) was diagnosed in pheasants on a gamebird rearing enterprise in south-west scotland. the gross pathology and bacteriological findings are described, as are the results of screening for s pullorum on the site in 1997. the causal organism was readily isolated from the lung, liver, yolk sac and heart blood on direct culture, but less readily from the digestive tract or by the use of selectiv ...199910204223
application of flagella-based immunoassays for serologic detection of salmonella pullorum infection in chickens.occasional salmonella pullorum outbreaks still occur in commercial poultry flocks despite widespread serologic testing, raising concerns about the efficacy of standard agglutination-based serologic assays for detecting infections with current field strains. the recent observation that many s. pullorum strains are recognized by antibodies to certain salmonella flagellar proteins suggests that similar antibodies might be elicited in infected birds. in the present study, flagella-based enzyme immun ...19989876853
growth of salmonella enteritidis and s. pullorum on hektoen agar and the expression of lipopolysaccharide or flagella.growth of strains of salmonella enteritidis and salmonella pullorum on hektoen agar has been reported to influence the expression of long-chain lipopolysaccharide and motility respectively. in this study we used a panel of strains of s. enteritidis and s. pullorum to investigate these phenomena. culture on hektoen agar did not cause rough strains of s. enteritidis to express long-chain lipopolysaccharide or strains of s. pullorum to become motile. it was concluded that growth of strains of s. en ...19989673020
serologic incidence of some diseases in kansas wild turkeys.wild turkeys (meleagris gallopavo, n = 1164) were tested for mycoplasma gallisepticum, mycoplasma meleagridis, mycoplasma synoviae, and salmonella pullorum from 1990 to 1997. although 3.3% of the turkeys were suspect for one or more diseases, only 0.9% were serologically positive for m. gallisepticum. these 11 positives were all from one country in south-central kansas.19989645333
status of salmonella gallinarum-pullorum infections in poultry in zambia.ten outbreaks of salmonella gallinarum-pullorum infections on poultry farms in zambia were investigated. three cases were seen in day-old broiler chickens and were diagnosed by culture as s. gallinarum-pullorum and characterized as pullorum disease because the mortality was only in the first few weeks. another case was diagnosed by culture from broiler parent stock. day-old chicks from two of the three cases were supplied by a hatchery. five cases in 5-to-18-month-old layer chickens were diagnos ...19979201422
detection of motility and putative synthesis of flagellar proteins in salmonella pullorum cultures.salmonella pullorum is a host-adapted pathogen of poultry previously thought to be nonmotile and nonflagellated. we discovered that motility can be induced in this organism under special medium conditions, and this motility was observed in 39 of 44 s. pullorum isolates tested. the migration appeared to occur only on the medium surface and not within the medium itself, indicating that swimming may not be responsible for this event. agar concentration, carbohydrate concentration and type, and temp ...19979157122
immune and physiological responses of turkeys with green-liver osteomyelitis complex.a study of field turkeys was undertaken in order to determine the involvement of relative immunological differences in the etiology of turkey osteomyelitis complex (toc). lame and normal turkeys were sampled from commercial flocks just prior to processing in two separate trials. after testing for functions of both humoral and cellular immunity, the turkeys were necropsied and examined for lesions of toc. there were significantly higher relative spleen and over weights and significantly lower bod ...19979057207
detecting infections of chickens with recent salmonella pullorum isolates using standard serological methods.despite extensive testing for salmonella pullorum infections in commercial poultry, occasional outbreaks of pullorum disease still occur. in some recent instances, questions have been raised about whether standard serological assays, which employ antigen strains selected several decades ago, are still able to detect currently prevalent s. pullorum strains. the present study evaluated the ability of standard rapid whole-blood plate agglutination test and serum tube agglutination test antigens to ...19979037683
comparison of modified tecra visual immunoassay with aoac method 989.14 and reference culture methods 967.25-967.28 for detection of salmonella in foods and related samples.a validation study was conducted to compare a new modified version of the tecra salmonella visual immunoassay (mod. via) with the original immunoassay (aoac method 989.14) and the reference culture methods (967.25-967.28). the mod. via was supplemented with polyclonal antibodies to salmonella pullorum and s. gallinarum, and compared favorably with aoac method 989.14 in sensitivity, inclusivity, and exclusivity studies. the ability of the mod. via to recover salmonella from artificially contamina ...19968946713
differentiation of salmonella gallinarum and salmonella pullorum by their whole-cell fatty acid methyl ester profiles.the whole-cell fatty acid methyl ester (fame) profiles of salmonella (s.) gallinarum and salmonella (s.) pullorum strains were compared in the computer-linked gas-liquid chromatography. the profiles of whole cellular fames allow the separation and identification s. gallinarum and s. pullorum by the microbial identification system and so can be used for their differentiation.19968794698
research note: differential chemotaxis of physarum polycephalum to salmonella gallinarum and salmonella pullorum.the chemotactic and chemotropic responses of the plasmodial stage of the slime mold physarum polycephalum were used to distinguish salmonella gallinarum and salmonella pullorum from 10 salmonella serovars that are commonly isolated from domestic poultry. utilizing an in vitro plasmodium agar plate assay method, p. polycephalum was attracted to s. gallinarum and s. pullorum, but the organism was repelled by salmonella derby, salmonella dublin, salmonella enteritidis, salmonella heidelberg, salmon ...19938479960
evolutionary origin and radiation of the avian-adapted non-motile salmonellae.multilocus enzyme electrophoresis was employed to estimate chromosomal genotypic diversity and relationships among 131 isolates of the non-motile salmonella biotypes gallinarum and pullorum (serotype 1, 9, 12:-:-) that cause fowl typhoid and pullorum disease, respectively. thirteen electrophoretic types (ets), marking clones, were distinguished, and construction of a neighbour-joining phylogenetic tree revealed three lineages: one consisted of five ets of gallinarum, a second included seven ets ...19938429538
bacteriological, serological, histological and immunohistochemical findings in laying hens with naturally acquired salmonella enteritidis phage type 4 infection.salmonella enteritidis phage type 4 was isolated from the reproductive tract of 10 (27 per cent) of 37 laying hens from three small flocks with a naturally acquired infection. the rapid slide agglutination test with salmonella pullorum-antigen was positive in 24 (64.9 per cent) of the 37 hens including the six hens in which the ovary was colonised and the 10 in which the oviduct was colonised. by immunohistochemical labelling s enteritidis was demonstrated in seven of eight culture-positive hens ...19938310606
salmonella pullorum infection and pheasants. 19938291181
pullorum disease with unusual signs in two backyard chicken flocks.atypical pullorum disease in two backyard chicken flocks occurred as sudden death of many adult birds in one backyard chicken flock and chronic wasting with high mortality in a second flock. chicks had been added to both flocks for several months before losses occurred. the chicks, which were not affected, came from a previously infected hatchery that did not totally depopulate.19938257389
evaluation of the humoral immune response to different antigens in arkansas regressor and progressor chickens.arkansas regressor and progressor chickens were re-evaluated for their immune response to different antigens. chickens received i.v. injection of either srbc (10 birds per line) or salmonella pullorum (sp; 10 birds per line) at 7 wk of age, and sera were collected at 6, 13, and 20 d postimmunization. a third group of birds (10 birds per line) received and i.m. injection of gat emulsion at 7 and 12 wk of age, and sera were collected at 10 and 14 wk of age. there were significant differences betwe ...19948146082
role of glycoconjugates in adherence of salmonella pullorum to the intestinal epithelium of chicks.1. the distribution of glycoconjugates on the surface of salmonella pullorum and the ileal epithelium of chicks was demonstrated by lectin cytochemistry. the role of glycoconjugates in adherence of s. pullorum to the ileal epithelium was determined by a sugar inhibition assay using the scanning electron microscope. 2. s. pullorum exhibited binding to concanavalin a (con a) and wheat germ agglutinin (wga) but not to soyabean agglutinin (sba), ricinus communis agglutinin (rca) and ulex europaeus a ...19957614028
report of pullorum disease in the somali democratic republic. 19817346909
a long-term serological study of a flock of chickens naturally infected with salmonella pullorum.the serological reactions of a group of chickens naturally infected with salmonella pullorum were observed for one year. the conventional whole blood test, rapid slide test and tube agglutination test were used in the study and compared with the microagglutination and microantiglobulin tests. results of the whole blood rapid slide and tube agglutination tests were as consistent as those produced with the microagglutination and microantiglobulin tests in detecting one third of the birds as reacto ...19817340098
[phagocytic activity and antibody formation in relation to the resistance of poultry to fowl typhoid-pullorum disease].vulchanov's method (1954) was employed to study the phagocytic activity, and the classic method with the agglutination test served to follow up the production of antibodies. some breed-associated differences were observed in the phagocytic activity and the normal serologic antibody titer prior to the infection with salmonella pullorum and salmonella gallinarum. highest were the indices with the leghorn breed, followed by the cornish and plymouth rock breeds. certain breed differences were also e ...19827170772
[poultry resistance to typhoid-pullorum disease by sex and breed].studied was the resistance of a total of 1729 birds of the leghorn, cornish, and white plymouth rock breeds with regard to typhoid and pullorum disease after the oral infection with strain 2348 of salmonella pullorum and strain 2307 of salmonella gallinarum. in accordance with the manifested clinical picture and the results of the bacteriologic investigations all birds were divided into three groups--unaffected, survived, and died. there existed breed- and sex-associated variations in the resist ...19827168143
the quantitative clearance of bacteria from the bloodstream of rainbow trout (salmo gairdneri).the blood clearance of 51cr-labelled heat-killed salmonella pullorum was biphasic and exponential for each phase. this pattern was the same for all three concentrations of bacteria used. the initial more rapid phase lasted for approximately 15 minutes when roughly 90% of the bacteria had been cleared. the second slower phase started 25 to 30 minutes post-inoculation and lasted for approximately 25 to 30 minutes. the tissue distribution of bacteria showed that in both absolute and relative terms, ...19827147628
[differentiation of immunoglobulin classes in the chicken].investigations were carried out to establish and differentiate the humoral immunoglobulins in spf-chickens, following an antigenic stimulus with salmonella pullorum. as many as five components were found in the blood sera of the test birds, which were shown to have immunoglobulin properties. they were differentiated as igm, iga, igg1, igg2, and igg3. those of the iga-type were found in exudate material from the abdominal cavity of birds infected with salmonella pullorum. in the course of the inf ...19827112920
the development of unusual b-cell functions in the testosterone-propionate-treated chicken.chickens were treated with 4 mg of testosterone propionate on the twelfth day of embryonic life. bursal remnants of testosterone-treated chickens were very small in size and had very few or no bursal follicles: the lymphoid tissue was replaced substantially by fibrosis. testosterone-treated chickens formed almost exclusively igm antibodies to sheep red blood cells and influenza virus, whereas no igm or igg response to brucella abortus or salmonella pullorum, and no igg response to sheep red bloo ...19806966602
development of delayed hypersensitivity in gnotobiotic mice.germ-free mice develop low levels of delayed hypersensitivity following exposure to sensitizing doses of sheep erythrocytes, compared to that seen in conventionally raised mice. infection of the germ-free animal with either salmonella gallinarum or salmonella pullorum was followed by extensive growth of the organisms within the intestine and the gut-associated lymphoid organs. as many as 5,000 viable salmonellae were recovered from the cecal and ileal peyer's patches as well as the mesenteric ly ...19806965376
survey of the health and husbandry of small poultry flocks in great britain.an investigation into the health and husbandry of 15 small poultry flocks was undertaken. each flock was visited in july and a questionnaire on management practices and disease history was completed. the flocks were clinically examined and serological tests for salmonella pullorum, mycoplasma gallisepticum, m synoviae, m meleagridis, newcastle disease, infectious bursal disease, infectious bronchitis, eggdrop syndrome 76, adenoviruses and reoviruses were carried out. oesophageal and cloacal swab ...19826814044
[ampicillin and rivanol in pullorum disease-fowl typhoid in chicks]. 19806771914
the effect of temperature and starvation on the clearance of bacteria from the bloodstream of rainbow trout (salmo gairdneri richardson).the blood clearance of 51cr-labeled heat-killed salmonella pullorum was generally biphasic and exponential for each phase. starvation had little significant effect on this pattern, although the rate of first phase clearance was probably slower. raising the water temperature from 8 degrees c to 18 degrees c enhanced the rate of clearance of the second phase to almost exactly double that at 8 degrees c. at 18 hr postinoculation, the spleen contained much more radioactivity per gm than any other ti ...19846584517
interaction of high temperature and salmonella pullorum antigen concentration on serum agglutinin and corticosteroid responses in white rock chickens.in three experiments, 6- to 7-week-old chickens were exposed to one or two standard heating episodes and were injected immediately afterward with different concentrations of heat-killed salmonella pullorum antigen (ag) or phosphate-buffered saline. the standard heat episode consisted of three .5-hr exposures of 44 to 46 c with .5-hr periods of 22 c between exposures. nonheated chickens were maintained at 22 c. when two heating episodes were used, there was a 12-hr interval between episodes. sera ...19846531335
immunological traits in generations 7 to 12 of two lines of japanese quail selected for high or low antibody response to newcastle disease virus.several immunological traits were compared in lines of japanese quail selected for high and low secondary immune responses to anti-newcastle disease virus (ndv). the high line of quail, selected for high ability to produce antibodies, was more responsive in both their primary response to ndv antigen used in the selection as well as to other antigens used in selection process including ndv (ishi strain), tissue culture-derived newcastle disease (tcnd strain), ndv strain b1, influenza virus, sheep ...19846473242
[erythrocyte antigen for the rapid intravital diagnosis of fowl typhoid and pullorum disease].a stable erythrocyte antigen was obtained for the rapid diagnosis of typhoid and pullorum disease in birds, which was tested under laboratory and field conditions. it was found that with the use of whole blood or serum taken from birds that had been either experimentally infected or had died of spontaneous infection of salmonella gallinarum and salmonella enteritidis the erythrocyte antigen was substantially superior to the routinely used colour ordinary test-antigen in terms of time consumed, i ...19836364560
concentration of salmonella pullorum antigen and the immunosuppressive effect of adrenocorticotropin in growing chickens.the role of antigen concentration on the immunosuppressive effect of adrenocorticotropin (acth) was tested in 6-week-old white rock chickens that were immunized i.v. with 1 ml of heat-treated salmonella pullorum at packed cell volume concentrations of .06 to .00015%, at 16 and 10 hr before the antigen (ag) injection, the birds received either 4 iu . 100 g body wt-1 of acth i.m., or the gelatin (gel) vehicle. total, 2-mercaptoethanol sensitive (2-mes), and 2-mercaptoethanol resistant (2-mer) aggl ...19836308589
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