Publications

biogenic amines derived from tryptophan in systemic and cutaneous scleroderma.	blood levels of serotonin (5-ht) and urinary excretion of 5-hydroxyindole-acetic acid (5-hiaa), tryptamine (t), and indole-acetic acid (iaa) were determined in 39 cases of systemic scleroderma and in 7 cases of very severe cutaneous scleroderma. the t/iaa ratio was normal and serotonin elevated in mild and rather severe acrosclerosis alike, i.e. in systemic scleroderma with pronounced vascular involvement. the t/iaa ratio was increased and serotonin normal in severe acroscleroderma, representing ...	1999	84460
prevalence of proteus species in urinary tract infections in a regional hospital in trinidad.	proteus bacteria are a well-known cause of urinary tract infections (utis). the prevalence of utis is high among catheterized patients and those undergoing manipulation of the urinary tract. this study assessed the prevalence of utis due to proteus species, the predisposing factors, complications and extent of antimicrobial resistance at a regional teaching hospital.	1999	10418178
tem-24 produced by four different species of enterobacteriaceae, including providencia rettgeri, in a single patient.	four species of members of the family enterobacteriaceae harboring extended-spectrum beta-lactamase (esbl) were recovered in a single patient hospitalized in an intensive care unit. among these isolates, we describe for the first time an esbl-producing providencia rettgeri strain. bacteria from the same species were shown to be genetically related by pulsed-field gel electrophoresis analysis. these strains produced the same tem derivative esbl, characterized as tem-24. this enzyme had the peculi ...	1999	10428940
molecular characterization of the genera proteus, morganella, and providencia by ribotyping.	the so-called proteus-providencia group is constituted at present by three genera and 10 species. several of the recognized species are common opportunistic pathogens for humans and animals. different methods based on the study of phenotypic characters have been used in the past with variable levels of efficiency for typing some species for epidemiological purposes. we have determined the rrna gene restriction patterns (ribotypes) for the type strains of the 10 different species of the genera pr ...	1999	10449462
development of bacterial resistance to the third generation cephalosporins and their clinical use.	development of gram-negative rods resistance to the third generation cephalosporins (cefotaxime, ceftazidime, cefoperazone) in connection with their application at the university hospital in olomouc was evaluated in this study. the highest increase in resistance to cefotaxime was detected in enterobacter cloacae (from 22.9% in 1995 to 49.0% in 1997) and enterobacter agglomerans strains (28.0% - 40.5%). in addition, increased resistance to ceftazidime in acinetobacter baumannii (12.5% - 35.1%), e ...	1999	10465127
crystal structure of penicillin g acylase from the bro1 mutant strain of providencia rettgeri.	penicillin g acylase is an important enzyme in the commercial production of semisynthetic penicillins used to combat bacterial infections. mutant strains of providencia rettgeri were generated from wild-type cultures subjected to nutritional selective pressure. one such mutant, bro1, was able to use 6-bromohexanamide as its sole nitrogen source. penicillin acylase from the bro1 strain exhibited an altered substrate specificity consistent with the ability of the mutant to process 6-bromohexanamid ...	1999	10548042
two cases of fatal transfusion-associated bacterial sepsis provoked by providencia rettgeri.		1999	10553171
synthesis and secretion of providencia rettgeri and escherichia coli heterodimeric penicillin amidases in saccharomyces cerevisiae.	the providencia rettgeri and escherichia coli pac genes encoding heterodimeric penicillin g amidases (pac) were successfully expressed in saccharomyces cerevisiae. furthermore, these recombinant enzymes are secreted from the yeast cell into the medium which is in contrast to bacterial hosts, where the enzymes are retained in the periplasm. contrary to the p. rettgeri pac-encoding gene, the e. coli pac is poorly expressed in yeast. the highest yield of p. rettgeri pac was obtained with a multi-co ...	1999	10072775
microbiology of the oil fly, helaeomyia petrolei.	helaeomyia petrolei larvae isolated from the asphalt seeps of rancho la brea in los angeles, calif., were examined for microbial gut contents. standard counts on luria-bertani, macconkey, and blood agar plates indicated ca. 2 x 10(5) heterotrophic bacteria per larva. the culturable bacteria represented 15 to 20% of the total population as determined by acridine orange staining. the gut itself contained large amounts of the oil, had no observable ceca, and maintained a slightly acidic ph of 6.3 t ...	1999	10103240
antibacterial activity of gentamicin and ciprofloxacin against gram-negative bacteria: interactions with pig and calf sera.	the antibacterial activity of pig and calf serum and its ability to interact with gentamicin and ciprofloxacin were studied in vitro using escherichia coli k-12, proteus rettgeri (sanelli) and klebsiella pneumoniae atcc 10031. the antimicrobial activity of the above drugs, alone or in combination with serum, was investigated by the checkerboard method and expressed as the minimal inhibitory concentration (microg ml-1). pig serum (25%) with gentamicin had a synergistic antibacterial effect agains ...	1999	10208763
an approach for enhancing heterologous production of providencia rettgeri penicillin acylase in escherichia coli.	heterologous production of providencia rettgeri penicillin acylase (pac) was optimized in escherichia coli. several factors, including carbon, temperature, and host effects, were identified to be critical for the enzyme overproduction. the optimum culture conditions for the enzyme production vary for different host/vector systems. with the optimization, both volumetric and specific pac activities could be significantly improved by more than 50-fold compared to the native expression in p. rettger ...	2000	10835229
in vitro activity of rifaximin, metronidazole and vancomycin against clostridium difficile and the rate of selection of spontaneously resistant mutants against representative anaerobic and aerobic bacteria, including ammonia-producing species.	rifaximin is a rifamycin derivative characterized by a wide antibacterial activity. this drug is neither absorbed by the gastrointestinal tract nor inactivated by gastric juices, and exerts its action entirely within the intestinal lumen.	2000	10859431
gut bacteria may be involved in interactions between plants, herbivores and their predators: microbial biosynthesis of n-acylglutamine surfactants as elicitors of plant volatiles.	n-acylamino acids are dominant and widespread constituents of insect oral secretions (regurgitants), serving the insect as biosurfactants in the digestive process. during feeding the conjugates may be introduced into damaged leaves and contribute there to the elicitation of plant defenses such as the induction of volatile biosynthesis. from gut segments of spodoptera exigua, mamestra brassicae and agrotis segetum 23 bacterial strains were isolated, ten of which were able to synthesise typical le ...	2000	11030433
natural antibiotic resistance of bacteria isolated from larvae of the oil fly, helaeomyia petrolei.	helaeomyia petrolei (oil fly) larvae inhabit the asphalt seeps of rancho la brea in los angeles, calif. the culturable microbial gut contents of larvae collected from the viscous oil were recently examined, and the majority (9 of 14) of the strains were identified as providencia spp. subsequently, 12 of the bacterial strains isolated were tested for their resistance or sensitivity to 23 commonly used antibiotics. all nine strains classified as providencia rettgeri exhibited dramatic resistance t ...	2000	11055901
diversity and contribution of the intestinal bacterial community to the development of musca domestica (diptera: muscidae) larvae.	the bacterial diversity in the intestinal tract of musca domestica l. was examined in larvae collected from turkey bedding and corn silage. aerobic culturing yielded 25 bacterial species, including 11 from larvae collected from turkey bedding and 14 from larvae collected from corn silage. providencia rettgeri (hadley, elkins & caldwell) was the only species common to both environments. two mammalian pathogens, yersinia pseudotuberculosis (pfeiffer) and ochrobactrum anthropi (holmes), were isolat ...	2000	11126551
pattern and flash visual evoked responses in multiple sclerosis.	in multiple sclerosis (ms), increase of delay may be important, but the earlier change is the behavior of the wave shape; amplitude is always lower even in ms patients without visual subjective symptomatology, provided not only flash but also pattern stimulation is performed. normal amplitude with pattern stimulation does not necessarily mean normal with flash. combining both stimulation methods, we obtained lowering of amplitude and change of wave shape in 100% of cases while latency was increa ...	2000	87495
fluorochrome staining of multilamellar liposomes.	multilamellar liposomes can be stained with such fluorochromes as acridine orange, eosin y, neutral red, and thiazine red. the liposomes are brought into a 1% solution of the fluorochrome; 5-10 minutes later they are centrifuged and washed by resuspending in water or phosphate buffered saline three times. the last pellet is resuspended and a drop studied with the fluorescence microscope (1000 x magnification). the fluorochrome is seen to be accumulated in the liposomal membranes. acridine orange ...	2000	89716
case report: treatment of posterior bite collapse with secondary occlusal traumatism.		2000	89659
the search for indices.		2000	91795
[nursing report. programs for clinical training of adult nursing (surgical nursing)].		2000	87545
progress with several models for the study of the sar of hallucinogenic agents.		2000	101879
[quantitative determination of copper in urine by color reaction with lead diethyldithiocarbamate].		2000	90204
in vitro analysis of allogeneic lymphocyte interaction. iv. dual recognition of b cell-associated mls locus and i-region determinants by a helper allogeneic effect factor (aef) generated across a minor h locus disparity.	a helper allogeneic effect factor (aef) was produced across an incompatibility at the minor histocompatibility loci. this aef is genetically restricted in its activity since it helps b cells only of the stimulator haplotype and of haplotypes that share both an mls and i-region identity with the stimulator haplotype. the i-region genes involved here map to the i-a and/or i-c subregions. an anti-lym immunoadsorbent column but neither an anti-h-2 nor an anti-ia column absorbed aef helper activity. ...	2000	87470
[madame g.'s hyperthyroidism].		2000	89684
the collective and the personal.		2001	90674
new trends in the preparation and storage of platelets.		2001	1731433
complications associated with therapeutic soft contact lenses.	the complications of therapeutic hydrophilic contact lens wear are systematically outlined. hypoxia is the major cause of initial patient failure as well as the long-term problems of corneal edema, neovascularization, and susceptibility to infection. the common difficulties with lens breakage, lens deposits, poor lens retention, and other entities are discussed. suggestions for avoiding and treating various complications are given, including updated indications and contraindications for fitting ...	2001	93261
tardive dyskinesia.		2001	90894
lymphocyte capping and carrier detection in duchenne muscular dystrophy.		2001	90791
research needs in low vision.	research needs are presented for several areas of low vision: (1) reading and learning, (2) new low-vision aids, (3) modifying the environment, (4) utilization of disciplines, (5) new technologies, and (6) integrating low-vision services.	2001	88182
in vitro and in vivo induction of effector t cells mediating dth responses to a protein and a synthetic polypeptide antigen.		2001	90558
prostatic acid phosphatase and prostatic cancer.		2001	88065
[complex intraoperative diagnosis of intracerebral tumors of the cerebral hemispheres].		2001	90438
[questions to television from psychopathology].	progress is expected in psychopathological diagnosis by using audiovisual procedures. nowadays, a valid psychopathology concept should be broadly planned and the important method of description as well as understanding procedures should be considered. for such a model of psychopathology it seems appropriate to investigate the different psychopathological phenomenons as expression of a disturbed communication ability. concerning this outlook, new strategies are to be developed or procedures of ot ...	2001	90381
needle biopsy of the pancreas.		2001	90278
oestrogen therapy and endometrial carcinoma.		2001	87699
formation of chromosomal tandem arrays of the sxt element and r391, two conjugative chromosomally integrating elements that share an attachment site.	the sxt element, a conjugative, self-transmissible, integrating element (a constin) originally derived from a vibrio cholerae o139 isolate from india, and incj element r391, originally derived from a south african providencia rettgeri isolate, were found to be genetically and functionally related. both of these constins integrate site specifically into the escherichia coli chromosome at an identical attachment site within the 5' end of prfc. they encode nearly identical integrases, which are req ...	2001	11157923
isolation and identification of bacteria associated with adult laboratory mexican fruit flies, anastrepha ludens (diptera: tephritidae).	from the guts of new and old colonies (female and male) of mexican fruit flies, anastrepha ludens (diptera: tephritidae), we identified a total of 18 different bacterial species belonging to the family enterobacteriaceae, pseudomonadaceae, vibrionaceae, micrococcaceae, deinococcacea, bacillaceae, and the genus listeria. enterobacter, providencia, serratia, and staphylococcus spp. were the most frequently isolated genera, with citrobacter, streptococcus, aerococcus, and listeria found less freque ...	2001	11178731
a case of purple urine bag syndrome associated with providencia rettgeri.		2001	11328846
antibiotic selective pressure and development of bacterial resistance.	this study evaluates the development of resistance in gram-negative rods to cefotaxime and ceftazidime, ofloxacin and ciprofloxacin, gentamicin and amikacin, meropenem and ampicillin/sulbactam over a five year period of use (1994-1998) at the university hospital in olomouc, czech republic. the development of bacterial resistance was linked with antibiotic use and hence selective pressure which was specific for the type of antibiotic and the bacterial species. statistically significant correlatio ...	2001	11337221
fluorogenic assay for penicillin g acylase activity.	a simple, highly sensitive, and rapid assay for high-throughput screening of penicillin g acylase-producing bacteria is presented. the method is based on the specific release of fluorescent 7-amino-4-methyl-coumarin through cleavage of phenylacetyl-4-methyl-coumaryl-7-amide by penicillin g acylase. the present method is suitable for screening pure enzymes as well as various penicillin g acylases like those from escherichia coli, proteus rettgeri, and kluyvera citrophila in cell extracts. in addi ...	2001	11355855
bacterial flora of the conjunctiva and nasal cavity in normal and diseased captive bustards.	a survey was carried out to describe the normal aerobic bacterial flora of the conjunctiva and nasal cavity of captive houbara bustards (chlamydotis undulata), kori bustards (ardeotis kori), and white-bellied bustards (eupodotis senegalensis) maintained at the national avian research center, abu dhabi, united arab emirates. a total of 58 samples were examined from the nasal cavity and 55 samples from the conjunctiva of healthy bustards. there was no bacterial growth in 45% of conjunctival sample ...	2001	11417826
activity of levofloxacin and ciprofloxacin against urinary pathogens.	this study compares the antibacterial activities of levofloxacin and ciprofloxacin against recently isolated urinary tract pathogens, by evaluating their mics and mbcs in accordance with nccls susceptibility tests, time-kill curves and interference with bacterial adhesion to uroepithelial cells. a total of 200 clinical isolates was tested, including the species escherichia coli, klebsiella pneumoniae, proteus mirabilis, proteus vulgaris, providencia rettgeri, pseudomonas aeruginosa, enterococcus ...	2001	11418511
[bacteremia due to providencia rettgeri].		2001	11440672
chemical composition and cytotoxic and antimicrobial activity of calycotome villosa (poiret) link leaves.	the chemical composition of the essential oil and methanol extract of calycotome villosa (poiret) link leaves collected in sardinia (italy) has been studied by analytical and spectroscopic methods. falcarinol and some alcohols, terpenes, furan derivatives, and paraffins have been isolated from the essential oil. thirteen alkaloids and falcarinol have been identified in the chloroform fraction of the basic methanol extract. six flavonoids and four anthraquinones have been isolated in the chlorofo ...	2001	11482772
hyperammonemic coma caused by providencia rettgeri infection in a child with prune belly syndrome.	objective: to report a case of hyperammonemic encephalopathy in the absence of liver disease caused by a urinary tract infection with a previously unreported organism. design: case report. setting: pediatric intensive care unit. patient: critically ill child who presented with hyperammonemic coma. intervention: bladder drainage and antimicrobial therapy for the urinary tract infection. measurements: rapid resolution of the hyperammonemia. main results: rapid improvement in the patient's neurolog ...	2001	12797879
high-level secretory expression of penicillin amidase from providencia rettgeri in saccharomyces cerevisiae: purification and characterization.	heterologous production of the heterodimeric penicillin g amidase (pac) from providencia rettgeri was optimized in saccharomyces cerevisiae. several factors, including the effect of different growth and induction conditions, were identified to be critical for the enzyme overproduction and secretion. the pac yield was significantly increased by more than 500-fold compared to that obtained in the native bacterium, and the recombinant enzyme was almost entirely secreted. electrophoretic characteriz ...	2002	11934304
effect of ph on high-temperature production of bacterial penicillin acylase in escherichia coli.	high-temperature-oriented production of bacterial penicillin acylase (pac), which is usually expressed at low temperatures (less than 30 degrees c), was demonstrated in this study via heterologous expression of the providencia rettgeri (p. rettgeri) pac gene in escherichia coli (e. coli). while it is possible to produce pac at a temperature as high as 37 degrees c, the environmental condition (specifically, culture ph) critically affected culture performance. production of pac at 37 degrees c wa ...	2002	12052092
bacterial population of a two-phase anaerobic digestion process treating effluent of cassava starch factory.	different groups of microorganisms in a two-phase anaerobic system were enumerated to evaluate the prevalence of specific groups and species. total and fecal coliforms showed similar values both in acidogenic and methanogenic reactors. the fecal streptococci were 4-fold higher in the acidogenic reactor, when compared with those of the methanogenic reactor. as expected, no methane forming or sulfate reducing bacteria were found in the acidogenic reactor. the populations of methanogenic bacteria w ...	2002	12088382
r391: a conjugative integrating mosaic comprised of phage, plasmid, and transposon elements.	the conjugative, chromosomally integrating element r391 is the archetype of the incj class of mobile genetic elements. originally found in a south african providencia rettgeri strain, r391 carries antibiotic and mercury resistance traits, as well as genes involved in mutagenic dna repair. while initially described as a plasmid, r391 has subsequently been shown to be integrated into the bacterial chromosome, employing a phage-like integration mechanism closely related to that of the sxt element f ...	2002	12193633
osteomyelitis associated with salmonella enterica ss arizonae in a colony of ridgenose rattlesnakes (crotalus willardi).	the identification of three arizona ridgenose rattlesnakes (crotalus willardi) with salmonella arizonae-associated osteomyelitis led to a 5-yr prospective study of radiographic signs and salmonella intestinal carriage rates in a 19-member colony of this rattlesnake species. ventrodorsal radiographs were performed and cloacal swabs were cultured for salmonella spp. annually. ten snakes survived the 5-yr period, with six of them remaining free of bony lesions. three snakes that had no bony lesions ...	2002	12564525
comparison of sxt and r391, two conjugative integrating elements: definition of a genetic backbone for the mobilization of resistance determinants.	the sxt element (sxt) is becoming an increasingly prevalent vector for the dissemination of antibiotic resistances in vibrio cholerae. sxt is a member of a larger family of elements, formerly defined as incj plasmids, that are self-transmissible by conjugation and integrate site-specifically into the host chromosome. comparison of the dna sequences of sxt and r391, an incj element from providencia rettgeri, indicate that these elements consist of a conserved backbone that mediates the regulation ...	2002	12568332
production of glycosylated thermostable providencia rettgeri penicillin g amidase in pichia pastoris.	penicillin g amidase from providencia rettgeri is a heterodimer of 92 kda. we have previously expressed the pr. rettgeri pac gene coding for this enzyme in saccharomyces cerevisiae, and now we report the expression and characterization in the methylotrophic yeast pichia pastoris. the recombinant catalytically active enzyme (rpac(pr)) was secreted from shake flask-grown p. pastoris cells into the medium at a level of approximately 0.18 u ml(-1). this yield of rpac(pr) was higher, by two orders of ...	2002	12702330
[primary disability of patients with bone fractures in saratov].		2002	126406
laparoscopy in hepatic disease.		2002	134923
design of living environments for nursing-home residents: increasing participation in recreation activities.	nursing-home residents have frequently been characterized as unoccupied and disengaged. at the outset of the present study, most residents were to be found in their own rooms, not exhibiting gross motor behavior or social interaction, and not participating in appropriate activities. to modify residents' levels of participation with the environment, a manipulative area was provided in the lounge. participation in the lounge averaged 20% on days when the activity was not available, but increased t ...	2002	126983
chemical hazards in the workplace.		2002	135215
[disabled children in the federal republic of germany in 1974 (author's transl)].	within the scope of an additional micro-census inquiry--carried out in 1974--the federal statistical office in wiesbaden assessed the number of physically, mentally and psychologically impaired children. the results are differentiated according to the types of disability and therapy, age, in-patient treatment and special school attendance. furthermore, the estimated results are compared with earlier surveys of the federal statistical office and other estimates.	2002	135318
resection of a massive arteriovenous malformation of the head and neck.	we report a patient with a massive arteriovenous malformation of the head and neck treated by surgical extirpation of the lesion. arteriography, hypotensive hypothermic anesthesia, and the intraoperative use of the doppler flowmeter were most helpful in this case.	2003	154679
[gastroschisis and omphalocele. treatment and results].		2003	150087
[relation between endocrinology and psychiatry. some recent developments].	relations between endocrinology and psychiatry are developing in two main directions: behavioural effects of hormones. after the effects of peripheral hormones, recent works are concerned with a.c.t.h. and antidiuretic hormone (which interfere with memory process) and with hypothalamic peptides (which might have psychotropic properties, independant of their endocrine regulation function). -correlations between psychiatric syndroms and endocrine disorders. in some mentally depressed patients, dis ...	2003	165466
contact dermatitis from polyfunctional acrylic monomers.	five out of 26 men employed formulating radiation drying printing ink developed eczematous dermatitis. positive patch tests were obtained with two polyfunctional acrylic monomers, pentaerythritol triacrylate and trimethylol propane triacrylate and with formulations containing these in four affected employees. cross sensitization was observed to dipentaerythritol monohydroxy pentaacrylate in each case. a fifth employee appeared to have developed irritant dermatitis from contact with these materia ...	2003	145343
the investigation of biopolymers in dilute solutions with x-ray small-angle scattering.		2003	201141
pseudogout: clinical observations and chemical analyses of deposits.		2003	181012
how does the depressed patient fare in a family practice?		2003	194945
[management of aplastic anemia in pregnancy and delivery].		2003	309014
incidence of dental caries in lucknow school-going children.		2003	296202
separation of tetracyclines by high-speed liquid chromatography.	the separation of tetracycline and its four commoninpurities has been studied by high-speed liquid chromatography. a preliminary study of the effectiveness of ion-exchange, adsorption, liquid-liquid partition and reversed-phase ion-pair chromatography indicated that only the last method showed promise. by developing special hydrocarbon-bonded stationary phases a rapid and complete resolution of all five tetracyclines has been obtained within 10 min. plate heights using a derivatised 18-mum parti ...	2003	166085
corneal manifestations of the systemic mucopolysaccharidoses.	newer biochemical understanding of the mucopolysaccharidoses now allows a better classification of these diseases. the dermatan and keratan sulfate-storing diseases have corneal clouding. the heparan sulfate-storing diseases have retinal changes and usually central nervous system manifestations.	2003	156516
the relationship of the pulp and periodontium.		2003	149297
improving the specific synthetic activity of a penicillin g acylase using dna family shuffling.	penicillin g acylas (pga) of providencia rettgeri (atcc 25599) was evolved using a modified dna family shuffling method. the identity of pga genes from escherichia coli, kluyvera citrophila and providencia rettgeri ranges from 62.5% to 96.9%. the pga genes from above three species were recombined and shuffled to create interspecies pga gene fusion libraries. by substituting assembled chimaeras for corresponding region of petppga, different recombinants were constructed and expressed in e. coli j ...	2003	12796820
prevalence of extended-spectrum beta-lactamases produced by nosocomial isolates of enterobacteriaceae in trakya university hospital, turkey.	the prevalence of extended-spectrum beta-lactamase (esbl) production by 194 nosocomial isolates of enterobacteriacea recovered from 1995 to 1999 was investigated. the esbl production was determined by the double-disk synergy test and was confirmed by the e-test esbl strip. twenty-three isolates (21 klebsiella pneumoniae, one escherichia coli, one providencia rettgeri) were found as esbl-producers (11.8%). these isolates were also usually resistant to non-betalactam antibiotics. most of them cont ...	2003	12901421
beta-lactamase stability of faropenem.	faropenem (far) is an orally available member of the penem class unique among carbapenems and other available beta-lactams. this study compared far to cephalosporins and imipenem with respect to beta-lactamase (bla) stability and emergence of resistance to staphylococcus aureus and escherichia coli. bla stability was studied using enzyme preparations from sonicated/centrifuged 24-hour cultures of e. coli, enterobacter cloacae, proteus vulgaris, providencia rettgeri, klebsiella pneumoniae, s. aur ...	2003	14504433
protease and phospholipase inhibition protect veneza zonata (hemiptera coreidae) against septicemia caused by parasite trypanosomatid 563dt.	veneza zonata (hemiptera coreidae) is an insect which causes losses in several crops, and it is also an important vector of lower trypanosomatids. v. zonata specimens were collected on rural properties in londrina, state of paraná, southern brazil. inoculation of leptomonas 563dt into v. zonata hemocoel caused insect death within approximately 24 h, with large bacterial proliferation into their hemocoels. some bacteria which were found in the digestive tract of those insects, such as escherichia ...	2004	14992855
cloning, overexpression, and characterization of a novel thermostable penicillin g acylase from achromobacter xylosoxidans: probing the molecular basis for its high thermostability.	the gene encoding a novel penicillin g acylase (pga), designated pgaw, was cloned from achromobacter xylosoxidans and overexpressed in escherichia coli. the pgaw gene contains an open reading frame of 2586 nucleotides. the deduced protein sequence encoded by pgaw has about 50% amino acid identity to several well-characterized pgas, including those of providencia rettgeri, kluyvera cryocrescens, and escherichia coli. biochemical studies showed that the optimal temperature for this novel pga (pga6 ...	2004	15128530
universal primer pcr with dgge for rapid detection of bacterial pathogens.	a universal primer pcr (uppcr) combined with denaturing gradient gel electrophoresis (dgge) was evaluated as a method permitting the rapid detection of pathogens. the results show that this method is efficient at amplifying the conserved regions of bacterial 16s rrna genes with universal primers and can detect causative bacterial pathogens rapidly. six species of bacteria from fisheries (pseudomonas fluorescens, vibrio anguillarum, aeromonas hydrophila, vibrio fluvialis, providencia rettgeri and ...	2004	15134888
per-1 production in a urinary isolate of providencia rettgeri.	a providencia rettgeri strain resistant to extended-spectrum cephalosporins and intermediate to aztreonam was isolated from the urine of a patient hospitalized in the urology clinic of ssk educational hospital in ankara. clavulanic acid restored the activity of extended-spectrum cephalosporins, suggesting that the strain was harboring an extended-spectrum beta-lactamase. since the per-1 enzyme is widespread in turkey, and had been already detected in a related species such as proteus mirabilis, ...	2004	15332708
metallo-beta-lactamase-producing gram-negative bacilli: laboratory-based surveillance in cooperation with 13 clinical laboratories in the kinki region of japan.	a total of 19,753 strains of gram-negative rods collected during two 6-month periods (october 2000 to march 2001 and november 2001 to april 2002) from 13 clinical laboratories in the kinki region of japan were investigated for the production of metallo-beta-lactamases (mbls). mbls were detected in 96 (0.5%) of the 19,753 isolates by the broth microdilution method, the 2-mercaptopropionic acid inhibition test, and pcr and dna sequencing analyses. mbl-positive isolates were detected in 9 of 13 lab ...	2004	15528723
cutaneous and pulmonary mycosis in green anacondas (euncectes murinus).	two dead, captive green anacondas (eunectes murinus), including one male and one female, submitted for necropsy were in poor body condition, having multiple, scattered, dark red foci on the scales and mottled lungs. both snakes had severe mycotic dermatitis. in addition, the male snake had mycotic stomatitis, and the female snake had mycotic pneumonia. trichophyton sp., verticillium sp., and alternaria sp. were isolated from the dermal lesions. the pulmonary lesions were morphologically consiste ...	2004	15732602
impaired lymphocyte transformation after accidental trauma.	the effect of accidental trauma on lymphocyte transformation induced by phytohaemagglutinin (pha), concanavalin a (con a), pokeweed mitogen (pwm) and purified protein derivative of tuberculin (ppd) was studied in 66 patients with minor, moderate or multiple trauma. an in vitro whole blood micromethod was used. the lymphocyte transformation induced by the mitogens and the antigen was impaired in patients with trauma in proportion to the severity of the injury. a decrease of lymphocytic responses ...	2004	525181
fetal alcohol syndrome.		2004	310188
hodgkin's disease in childhood.	the histopathologic study of 103 patients with hodgkin's disease including 5 cases who had staging laparotomy during the last 10 years were reviewed. the following conclusions were drawn: 1. no significant sex difference among subtypes were noted. 2. associated glomerulopathy, nephrotic syndromes, and amyloidosis were occasionally found. 3. in childhood lymphomas excluding leukemia are the most frequent malignacies. among the lymphomas, hodgkin's disease predominates. 4. the most frequent subtyp ...	2004	387804
intra-osseous lipoma of the proximal part of the femur. case report.		2004	374417
[intracellular mechanisms of cholestasis].		2005	3320466
achievement award 1976: the robert wood johnson foundation.		2005	1065210
effects of professional and media warnings about the association between aspirin use in children and reye's syndrome.	the media played a central role in changing the use of aspirin among children with viral illness following reports of its association with a rare but deadly disease, reye's syndrome (rs). it did so by alerting health professionals and parents about ways to prevent rs in children. indeed, by the time aspirin product labeling was required by the fda in 1986, most of the decline in rs incidence had already occurred. in the past, media-only health education campaigns have been relatively unsuccessfu ...	2005	1316991
letter: restless legs: precipitated by beta blockers, relieved by orphenadrine.		2005	1214686
synthesis of heparin saccharides iii. synthesis of derivatives of d-glucosamine as starting materials for disaccharides.		2005	1176303
functional states of various heart segments during protracted hypokinesis in patients with tuberculosis of bones and joints.	by the method of acceleration cinecardiography the effect of hypokinesis on the contracting activity of the left and right heart was studied in 46 patients with tuberculous affections of bones and joints, confined to strict bed rest. as controls here served 40 patients with unrestricted mobility and 33 healthy subjects. it was found that the restriction of mobility and compulsory horizontal body positions of the patients contributed to a rearrangement of the phasic structure of the cardiac cycle ...	2005	544173
management of pathological fractures at the mayo clinic.		2005	1234120
the differentiation of cytotoxic t lymphocytes in vitro: an ultrastructural study.	t killer cells were identified morphologically in two different culture systems. the first system allowed identification by comparing under identical culture conditions two types of stimulation, one leading to cytotoxic activity levels that were very high (h-2 stimulation) and the other to levels which were practically nil (m-locus stimulation). a second system allowed identification by the use of a collaborative system in which relatively pure precursors of t killer cells were mixed with precur ...	2005	1082798
screening of antibiotics resistance to enterobacteriaceae, pseudomonas aeruginosa, and acinetobacter baumannii by an advanced expert system.	the vitek2 advanced expert system (aes) gives information about the antibiotics-resistance mechanisms based on the biological validation derived from the vitek2 susceptibility result. in this study, we investigated whether or not this system correctly categorized the beta-lactamase resistance mechanism data derived from the vitek2 susceptibility result using the testing card, ast-n025, with enterobacteriaceae, pseudomonas aeruginosa, and acinetobacter baumannii. we used 131 strains, and their ph ...	2005	16369735
antimicrobial activity of flavonoids from pelargonium radula (cav.) l'hérit.	flavonoids from pelargonium radula (cav.) l'hérit were purified by column chromatography. two fractions were obtained: f1 (main flavonoid isoquercitrin) and f2 (main flavonoid rutin). in vitro antimicrobial activity of f1 and f2 were tested against eleven species of bacteria and eleven species of fungi. both fractions demonstrated strong inhibitory activity against staphylococcus aureus, proteus rettgeri, candida tropicalis and microsporum gypseum. staphylococcus sp. (coagulase-negative) and can ...	2005	16375833
recruiting and retaining minority students in health sciences training programs.		2005	1195416
vitamins and cancer.		2005	1196282
uterine fibroid: an unusual cause of recurrent haemorrhagic pleural effusion.	a 51-year-old woman presented with dyspnoea due to pleural effusion, which repeatedly reaccumulated rapidly after tapping. a pelvic mass was present and she was considered to have disseminated ovarian malignant tumour. total hysterectomy with bilateral salpingo-oophorectomy was performed, and the tumour was shown to be a necrotic uterine fibromyoma. after the operation the patient has been well, with no recurrence of the effusion. this is the twelfth reported case of the association of hydrothor ...	2005	1196225
[pre- and postoperative radiotherapy of laryngeal cancer].		2005	1196026
[a case of giant cell tumor of the maxilla associated with paget's disease (author's transl)].		2005	1087670
social class and preschool language skill: v. cognitive and semantic mastery of number.	this study was done to test the validity of two hypotheses of why children, and especially black children, of families of the poverty sector commonly fail in school. sociologists deny for them either a cognitive or a linguistic deficit, and blame their failures on having to master the unfamiliar dialect of standard english while learning to read. the authors, on the other hand, blame the failures on inadequacies of early preschool experience that result in such deficits as one in semantic master ...	2005	1175915
oxalate-degrading providencia rettgeri isolated from human stools.	oxalate-degrading bacteria are thought to metabolize intestinal oxalate and thus decrease the urinary excretion of oxalate by reducing its intestinal absorption.	2005	15985073