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[bacterial resistance to antibiotics in the czech republic. the working group for monitoring resistance in the czech republic].in 1991 the authors made an investigation on the resistance of clinically important bacteria. seventeen antibiotic centres in the czech republic participated in the investigation. the resistance to antibiotics of 32,567 strains of twelve bacterial species isolated from hospitalized patients depends on 1. the type of bacteria and antibiotics, 2. the source (specimen of pathological material) and 3. the locality (hospital). the resistance of staphylococcus aureus to oxacillin with the exception of ...19948111828
predictive accuracy of disk diffusion test for proteus vulgaris and providencia species against five newer orally administered cephalosporins, cefdinir, cefetamet, cefprozil, cefuroxime, and loracarbef.three members of the tribe proteeae (proteus vulgaris, providencia rettgeri, and providencia stuartii) were tested against five newer orally administered cephalosporins (cefdinir, cefprozil, cefuroxime, cefetamet, and loracarbef) by the disk diffusion and reference broth microdilution methods. one hundred strains of these organisms were tested to confirm the excessive interpretive error rates that previously had been noted for the disk diffusion test. the results indicate that the suggested disk ...19948150976
rapid emergence of quinolone resistance in cirrhotic patients treated with norfloxacin to prevent spontaneous bacterial peritonitis.we carried out quantitative culturing of stools from 31 hospitalized alcoholic patients with cirrhosis and ascites, before treatment with 400 mg of norfloxacin per day, weekly for the first month, and then every 2 weeks thereafter for 15 to 229 days (median, 54 days). members of the family enterobacteriaceae virtually disappeared from the stools (< 10(2)/g), but treatment had little effect on enterococci. no selection of resistant organisms occurred in 15 patients, but the remaining 16 patients ...19948192461
in vitro activity of cefpodoxime compared with other oral cephalosporins tested against 5556 recent clinical isolates from five medical centers.a multicenter study was conducted in which the in vitro activity of cefpodoxime (the active metabolite of the prodrug ester cefpodoxime proxetil) was compared with those of cefixime, cefuroxime, cefaclor, cefadroxil, and clarithromycin against 5556 recent clinical isolates. cefpodoxime demonstrated potent activity against members of the enterobacteriaceae, in particular against species generally resistant to the established oral cephalosporins such as proteus vulgaris [minimum inhibitory concent ...19938243035
[the antimicrobial activity of cefteram against recently clinically detected and isolated strains].in order to examine antibiotic activities of cefteram (cftm), its minimum inhibitory concentrations (mic's) and those of other cephem drugs were determined against clinically isolated strains received from july 1990 to june 1991 and from july 1992 to february 1993 from medical facilities throughout the country and against clinically isolated strains detected in our laboratory in samples from patients with various infectious diseases. the obtained results are summarized below. 1. no cftm-resistan ...19938254900
in vitro antimicrobial activities of lactoferrin, its concomitant use with cefpodoxime proxetil and clinical effect of cefpodoxime proxetil.as one of the biodefense mechanisms, lactoferrin (lfn) in the secreta of female genital organ may be an interesting biological material in view of its antimicrobial activity. in the present study, we investigated antimicrobial activities of lfn and its combination with cefpodoxime proxetil (cpdx-pr), we also as evaluated clinical effect of cpdx-pr. the following results were obtained. 1. antimicrobial activities of lfn were tested against 15 strains of 10 species of bacteria, and potent activiti ...19938360983
potent inhibitory action of the gastric proton pump inhibitor lansoprazole against urease activity of helicobacter pylori: unique action selective for h. pylori cells.the gastric proton pump inhibitor lansoprazole, its active analog ag-2000, and omeprazole dose dependently inhibited urease activity extracted with distilled water from helicobacter pylori cells; the 50% inhibitory concentrations were between 3.6 and 9.5 microm, which were more potent than those of urease inhibitors, such as acetohydroxamic acid, hydroxyurea, and thiourea. these compounds also inhibited urease activity in intact cells of h. pylori and helicobacter mustelae but did not inhibit ur ...19938494373
sequence of the providencia rettgeri lexa gene and its control region. 19938502572
[antimicrobial susceptibility patterns of the proteeae in japan, 1989].we discussed the antimicrobial susceptibilities of proteeae isolated in japan, 1989. eight hundred six clinical isolates were collected from 47 hospitals. these were comprised of 431 strains of proteus mirabilis, 155 proteus vulgaris, 154 morganella morganii, 44 providencia rettgeri and 22 providencia stuartii. antibiotics tested in this study were 2 penicillins, 5 cephems, 1 carbapenem and 2 aminoglycosides. the mic's were determined using the standard method of the japan society of chemotherap ...19938515560
[gram-negative bacteria contaminating the process of producing lysine].the authors have isolated gram-negative bacteria of the enterobacteriaceae family from the culture liquid of industrial fermenters with low yield of lysine. most of them possessed the characters typical of klebsiella pneumoniae and escherichia coli, the rest were identified as the representatives of genera proteus, providencia, enterobacter, hafnia. strains of klebsiella pneumoniae, e.coli, proteus rettgeri manifested lysine-decarboxylase activity. the capacity of some strains to destruct lysine ...19958563944
[in vitro antibacterial activity of a new parenteral penem, sulopenem].eighty percent minimum inhibitory concentrations (mic80) of sulopenem against clinically isolated 12 to 80 strains of each of different bacteria were as follows: methicillin-susceptible staphylococcus aureus (mssa): 0.20 micrograms/ml, methicillin-resistant s. aureus (mrsa): 50 micrograms/ml, coagulase-negative staphylococci: 3.13 micrograms/ml, streptococcus pyogenes: < or = 0.013 microgram/ml, streptococcus pneumoniae: < or = 0.013 microgram/ml, beta-streptococci: 0.05 microgram/ml, enterococc ...19968786624
antimicrobial activity of human pancreatic juice and its interaction with antibiotics.pancreatic juice (pj) should be a factor of variability in the antimicrobial activity of antibiotics eliminated by the pancreas during pancreatic infections. we studied its effects on the activity of antimicrobial drugs with different mechanisms of action. samples of pure pj were collected from 16 patients with stabilized external pancreatic fistulas. the antimicrobial activity of the juice at different concentrations (from 1.25 to 100%) alone and in combination with mezlocillin, imipenem, ceftr ...19968878588
providencia rettgeri meningitis in hatchling saltwater crocodiles (crocodylus porosus). 19968941426
the prevalence of gentamicin 2'-n-acetyltransferase in the proteeae and its role in the o-acetylation of peptidoglycan.the prevalence of aac(2')-ia, a gene coding for gentamicin 2'-n-acetyltransferase in providencia stuartii, among species of the proteeae was investigated to determine if it is a common resistance factor and whether the correlation observed in p. stuartii between its expression and the levels of peptidoglycan o-acetylation represents a general feature of bacteria producing this form of modified peptidoglycan. an evaluation of the mics of gentamicin for each of the species of the proteeae did not ...19968961557
[meropenem: microbiologic perspective].meropenem is a beta-lactamic carbapenem derived from thienamycin and is structurally characterized by the presence of a beta-methyl group in position c1 which confers stability to the molecule versus renal dehydropeptidase 1 (dhp-1), thereby making the coadministration of an enzyme inhibitor unnecessary. its esterochemical configuration of the lateral chain in c2 (dimethyl carbomoilpyrrolidenethium) increases the activity versus gram negative bacteria (enterobacteria and pseudomonas) and moreove ...19979410064
in vitro and in vivo antibacterial activities of gv129606, a new broad-spectrum trinem.gv129606 is a new parenteral trinem antibiotic belonging to the beta-lactam class. it combines broad-spectrum activity (against gram-negative and -positive bacteria, aerobes and anaerobes), with high potency and resistance to beta-lactamases. comparative in vitro and in vivo antibacterial activities were determined for gv129606 against more than 400 recent clinical isolates (aerobes, including beta-lactamase producers, and anaerobes), using representative antibacterial agents (meropenem, piperac ...19979420050
[urinary bacteria in elderly patients with urinary incontinence and low levels of daily activity].the results of bacteria isolated from the urine of inactive elderly patients with urinary incontinence in 1982, 87, 93 and 95 were compared and the chronological changes of urinary bacteria were analyzed. the following results were obtained: (1) the most common bacteria isolated from the patients in 1995 were d-group enterococcus, followed by providencia rettgeri, e. coli, pseudomonas aeruginosa and proteus mirabilis. it is thought that the main cause of the more frequent isolation of enterococc ...19979493467
antibacterial activity of peritoneal exudate in patients treated with 2 g cefotiam for surgical anti-microbial prophylaxis.the objective of this study was to investigate the presence of antibacterial activity in peritoneal exudate (pe) of patients treated with cefotiam (cft). cft (2 g) was administered as a 'single-shot' antimicrobial prophylaxis to 6 patients at the beginning of colorectal resection. samples of pe were collected from each patient on days 1, 2 and 3 after surgery. cft was detectable in the samples of day 1 for 5 of the 6 patients. the influence of pe on antibacterial activity of the antimicrobial dr ...19989612603
[bactericidal activity and capacity for selection of mutants resistant to imipenmen and meropenem in strains of proteus, morganella and providencia].to compare the bactericidal activity and frequency of mutation for meropenem and imipenem against proteus mirabilis, morganella morganii and providencia rettgeri.19989763741
studies on the formation of crystalline bacterial biofilms on urethral catheters.a model of the catheterised bladder was used to test the ability of urease-producing urinary tract pathogens to encrust urethral catheters. encrustation was assessed by determining the amounts of calcium and magnesium deposited on the catheters and visualised by scanning electron microscopy. urease-positive morganella morganii, klebsiella pneumoniae, and pseudomonas aeruginosa failed to raise the urinary ph and form crystalline biofilms. in contrast, strains of proteus mirabilis, proteus vulgari ...19989832268
natural antibiotic susceptibility of providencia stuartii, p. rettgeri, p. alcalifaciens and p. rustigianii strains.the natural antibiotic susceptibility of 38 providencia rettgeri, 35 p. stuartii, 23 p. alcalifaciens and 20 p. rustigianii strains was examined. mic values were determined by a microdilution procedure and evaluated by a table calculation programme. p. stuartii was the least susceptible providencia sp. and was naturally resistant to tetracyclines, some penicillins, older cephalosporins, sulphamethoxazole and fosfomycin and to antibiotics to which other species of enterobacteriaceae are also resi ...19989839568
antibacterial activity of human pleural fluid: alone and in combination with antibiotics.this study investigated the antibacterial activity of human pleural fluid (hpf) and its interaction with gentamicin (gm), meropenem (mrpm), ciprofloxacin (cpfx) and clarithromycin (cltm) against escherichia coli k-12, proteus rettgeri (sanelli) and staphylococcus aureus. minimal inhibitory concentrations or volumes, expressed as mic or volume percentage (miv, v/v%), were measured using a micro-dilution technique in microtiter plates. the antimicrobial activity of hpf combinations with antimicrob ...19989916908
synthesis and secretion of providencia rettgeri and escherichia coli heterodimeric penicillin amidases in saccharomyces cerevisiae.the providencia rettgeri and escherichia coli pac genes encoding heterodimeric penicillin g amidases (pac) were successfully expressed in saccharomyces cerevisiae. furthermore, these recombinant enzymes are secreted from the yeast cell into the medium which is in contrast to bacterial hosts, where the enzymes are retained in the periplasm. contrary to the p. rettgeri pac-encoding gene, the e. coli pac is poorly expressed in yeast. the highest yield of p. rettgeri pac was obtained with a multi-co ...199910072775
microbiology of the oil fly, helaeomyia petrolei.helaeomyia petrolei larvae isolated from the asphalt seeps of rancho la brea in los angeles, calif., were examined for microbial gut contents. standard counts on luria-bertani, macconkey, and blood agar plates indicated ca. 2 x 10(5) heterotrophic bacteria per larva. the culturable bacteria represented 15 to 20% of the total population as determined by acridine orange staining. the gut itself contained large amounts of the oil, had no observable ceca, and maintained a slightly acidic ph of 6.3 t ...199910103240
antibacterial activity of gentamicin and ciprofloxacin against gram-negative bacteria: interactions with pig and calf sera.the antibacterial activity of pig and calf serum and its ability to interact with gentamicin and ciprofloxacin were studied in vitro using escherichia coli k-12, proteus rettgeri (sanelli) and klebsiella pneumoniae atcc 10031. the antimicrobial activity of the above drugs, alone or in combination with serum, was investigated by the checkerboard method and expressed as the minimal inhibitory concentration (microg ml-1). pig serum (25%) with gentamicin had a synergistic antibacterial effect agains ...199910208763
asymptomatic bacteriuria in pregnancy: epidemiological, clinical and microbiological approach.asymptomatic urinary tract infection is a risk factor for fetal and maternal morbidity including development of pyelonephritis, premature labor and impaired intra-uterine development. in this study, 326 pregnant and 100 non-pregnant control women were screened for significant asymptomatic bacteriuria from april 8 to july 25, 1997 to gain insight into the prevalence rate, clinical characteristics of the disease and microbiological assessments of the causative agents. all the subjects were clinica ...199810214459
prevalence of proteus species in urinary tract infections in a regional hospital in trinidad.proteus bacteria are a well-known cause of urinary tract infections (utis). the prevalence of utis is high among catheterized patients and those undergoing manipulation of the urinary tract. this study assessed the prevalence of utis due to proteus species, the predisposing factors, complications and extent of antimicrobial resistance at a regional teaching hospital.199910418178
tem-24 produced by four different species of enterobacteriaceae, including providencia rettgeri, in a single patient.four species of members of the family enterobacteriaceae harboring extended-spectrum beta-lactamase (esbl) were recovered in a single patient hospitalized in an intensive care unit. among these isolates, we describe for the first time an esbl-producing providencia rettgeri strain. bacteria from the same species were shown to be genetically related by pulsed-field gel electrophoresis analysis. these strains produced the same tem derivative esbl, characterized as tem-24. this enzyme had the peculi ...199910428940
molecular characterization of the genera proteus, morganella, and providencia by ribotyping.the so-called proteus-providencia group is constituted at present by three genera and 10 species. several of the recognized species are common opportunistic pathogens for humans and animals. different methods based on the study of phenotypic characters have been used in the past with variable levels of efficiency for typing some species for epidemiological purposes. we have determined the rrna gene restriction patterns (ribotypes) for the type strains of the 10 different species of the genera pr ...199910449462
development of bacterial resistance to the third generation cephalosporins and their clinical use.development of gram-negative rods resistance to the third generation cephalosporins (cefotaxime, ceftazidime, cefoperazone) in connection with their application at the university hospital in olomouc was evaluated in this study. the highest increase in resistance to cefotaxime was detected in enterobacter cloacae (from 22.9% in 1995 to 49.0% in 1997) and enterobacter agglomerans strains (28.0% - 40.5%). in addition, increased resistance to ceftazidime in acinetobacter baumannii (12.5% - 35.1%), e ...199910465127
crystal structure of penicillin g acylase from the bro1 mutant strain of providencia rettgeri.penicillin g acylase is an important enzyme in the commercial production of semisynthetic penicillins used to combat bacterial infections. mutant strains of providencia rettgeri were generated from wild-type cultures subjected to nutritional selective pressure. one such mutant, bro1, was able to use 6-bromohexanamide as its sole nitrogen source. penicillin acylase from the bro1 strain exhibited an altered substrate specificity consistent with the ability of the mutant to process 6-bromohexanamid ...199910548042
two cases of fatal transfusion-associated bacterial sepsis provoked by providencia rettgeri. 199910553171
an approach for enhancing heterologous production of providencia rettgeri penicillin acylase in escherichia coli.heterologous production of providencia rettgeri penicillin acylase (pac) was optimized in escherichia coli. several factors, including carbon, temperature, and host effects, were identified to be critical for the enzyme overproduction. the optimum culture conditions for the enzyme production vary for different host/vector systems. with the optimization, both volumetric and specific pac activities could be significantly improved by more than 50-fold compared to the native expression in p. rettger ...200010835229
in vitro activity of rifaximin, metronidazole and vancomycin against clostridium difficile and the rate of selection of spontaneously resistant mutants against representative anaerobic and aerobic bacteria, including ammonia-producing species.rifaximin is a rifamycin derivative characterized by a wide antibacterial activity. this drug is neither absorbed by the gastrointestinal tract nor inactivated by gastric juices, and exerts its action entirely within the intestinal lumen.200010859431
gut bacteria may be involved in interactions between plants, herbivores and their predators: microbial biosynthesis of n-acylglutamine surfactants as elicitors of plant volatiles.n-acylamino acids are dominant and widespread constituents of insect oral secretions (regurgitants), serving the insect as biosurfactants in the digestive process. during feeding the conjugates may be introduced into damaged leaves and contribute there to the elicitation of plant defenses such as the induction of volatile biosynthesis. from gut segments of spodoptera exigua, mamestra brassicae and agrotis segetum 23 bacterial strains were isolated, ten of which were able to synthesise typical le ...200011030433
natural antibiotic resistance of bacteria isolated from larvae of the oil fly, helaeomyia petrolei.helaeomyia petrolei (oil fly) larvae inhabit the asphalt seeps of rancho la brea in los angeles, calif. the culturable microbial gut contents of larvae collected from the viscous oil were recently examined, and the majority (9 of 14) of the strains were identified as providencia spp. subsequently, 12 of the bacterial strains isolated were tested for their resistance or sensitivity to 23 commonly used antibiotics. all nine strains classified as providencia rettgeri exhibited dramatic resistance t ...200011055901
diversity and contribution of the intestinal bacterial community to the development of musca domestica (diptera: muscidae) larvae.the bacterial diversity in the intestinal tract of musca domestica l. was examined in larvae collected from turkey bedding and corn silage. aerobic culturing yielded 25 bacterial species, including 11 from larvae collected from turkey bedding and 14 from larvae collected from corn silage. providencia rettgeri (hadley, elkins & caldwell) was the only species common to both environments. two mammalian pathogens, yersinia pseudotuberculosis (pfeiffer) and ochrobactrum anthropi (holmes), were isolat ...200011126551
formation of chromosomal tandem arrays of the sxt element and r391, two conjugative chromosomally integrating elements that share an attachment site.the sxt element, a conjugative, self-transmissible, integrating element (a constin) originally derived from a vibrio cholerae o139 isolate from india, and incj element r391, originally derived from a south african providencia rettgeri isolate, were found to be genetically and functionally related. both of these constins integrate site specifically into the escherichia coli chromosome at an identical attachment site within the 5' end of prfc. they encode nearly identical integrases, which are req ...200111157923
isolation and identification of bacteria associated with adult laboratory mexican fruit flies, anastrepha ludens (diptera: tephritidae).from the guts of new and old colonies (female and male) of mexican fruit flies, anastrepha ludens (diptera: tephritidae), we identified a total of 18 different bacterial species belonging to the family enterobacteriaceae, pseudomonadaceae, vibrionaceae, micrococcaceae, deinococcacea, bacillaceae, and the genus listeria. enterobacter, providencia, serratia, and staphylococcus spp. were the most frequently isolated genera, with citrobacter, streptococcus, aerococcus, and listeria found less freque ...200111178731
a case of purple urine bag syndrome associated with providencia rettgeri. 200111328846
antibiotic selective pressure and development of bacterial resistance.this study evaluates the development of resistance in gram-negative rods to cefotaxime and ceftazidime, ofloxacin and ciprofloxacin, gentamicin and amikacin, meropenem and ampicillin/sulbactam over a five year period of use (1994-1998) at the university hospital in olomouc, czech republic. the development of bacterial resistance was linked with antibiotic use and hence selective pressure which was specific for the type of antibiotic and the bacterial species. statistically significant correlatio ...200111337221
fluorogenic assay for penicillin g acylase activity.a simple, highly sensitive, and rapid assay for high-throughput screening of penicillin g acylase-producing bacteria is presented. the method is based on the specific release of fluorescent 7-amino-4-methyl-coumarin through cleavage of phenylacetyl-4-methyl-coumaryl-7-amide by penicillin g acylase. the present method is suitable for screening pure enzymes as well as various penicillin g acylases like those from escherichia coli, proteus rettgeri, and kluyvera citrophila in cell extracts. in addi ...200111355855
bacterial flora of the conjunctiva and nasal cavity in normal and diseased captive bustards.a survey was carried out to describe the normal aerobic bacterial flora of the conjunctiva and nasal cavity of captive houbara bustards (chlamydotis undulata), kori bustards (ardeotis kori), and white-bellied bustards (eupodotis senegalensis) maintained at the national avian research center, abu dhabi, united arab emirates. a total of 58 samples were examined from the nasal cavity and 55 samples from the conjunctiva of healthy bustards. there was no bacterial growth in 45% of conjunctival sample ...200111417826
activity of levofloxacin and ciprofloxacin against urinary pathogens.this study compares the antibacterial activities of levofloxacin and ciprofloxacin against recently isolated urinary tract pathogens, by evaluating their mics and mbcs in accordance with nccls susceptibility tests, time-kill curves and interference with bacterial adhesion to uroepithelial cells. a total of 200 clinical isolates was tested, including the species escherichia coli, klebsiella pneumoniae, proteus mirabilis, proteus vulgaris, providencia rettgeri, pseudomonas aeruginosa, enterococcus ...200111418511
[bacteremia due to providencia rettgeri]. 200111440672
chemical composition and cytotoxic and antimicrobial activity of calycotome villosa (poiret) link leaves.the chemical composition of the essential oil and methanol extract of calycotome villosa (poiret) link leaves collected in sardinia (italy) has been studied by analytical and spectroscopic methods. falcarinol and some alcohols, terpenes, furan derivatives, and paraffins have been isolated from the essential oil. thirteen alkaloids and falcarinol have been identified in the chloroform fraction of the basic methanol extract. six flavonoids and four anthraquinones have been isolated in the chlorofo ...200111482772
high-level secretory expression of penicillin amidase from providencia rettgeri in saccharomyces cerevisiae: purification and characterization.heterologous production of the heterodimeric penicillin g amidase (pac) from providencia rettgeri was optimized in saccharomyces cerevisiae. several factors, including the effect of different growth and induction conditions, were identified to be critical for the enzyme overproduction and secretion. the pac yield was significantly increased by more than 500-fold compared to that obtained in the native bacterium, and the recombinant enzyme was almost entirely secreted. electrophoretic characteriz ...200211934304
effect of ph on high-temperature production of bacterial penicillin acylase in escherichia coli.high-temperature-oriented production of bacterial penicillin acylase (pac), which is usually expressed at low temperatures (less than 30 degrees c), was demonstrated in this study via heterologous expression of the providencia rettgeri (p. rettgeri) pac gene in escherichia coli (e. coli). while it is possible to produce pac at a temperature as high as 37 degrees c, the environmental condition (specifically, culture ph) critically affected culture performance. production of pac at 37 degrees c wa ...200212052092
bacterial population of a two-phase anaerobic digestion process treating effluent of cassava starch factory.different groups of microorganisms in a two-phase anaerobic system were enumerated to evaluate the prevalence of specific groups and species. total and fecal coliforms showed similar values both in acidogenic and methanogenic reactors. the fecal streptococci were 4-fold higher in the acidogenic reactor, when compared with those of the methanogenic reactor. as expected, no methane forming or sulfate reducing bacteria were found in the acidogenic reactor. the populations of methanogenic bacteria w ...200212088382
r391: a conjugative integrating mosaic comprised of phage, plasmid, and transposon elements.the conjugative, chromosomally integrating element r391 is the archetype of the incj class of mobile genetic elements. originally found in a south african providencia rettgeri strain, r391 carries antibiotic and mercury resistance traits, as well as genes involved in mutagenic dna repair. while initially described as a plasmid, r391 has subsequently been shown to be integrated into the bacterial chromosome, employing a phage-like integration mechanism closely related to that of the sxt element f ...200212193633
osteomyelitis associated with salmonella enterica ss arizonae in a colony of ridgenose rattlesnakes (crotalus willardi).the identification of three arizona ridgenose rattlesnakes (crotalus willardi) with salmonella arizonae-associated osteomyelitis led to a 5-yr prospective study of radiographic signs and salmonella intestinal carriage rates in a 19-member colony of this rattlesnake species. ventrodorsal radiographs were performed and cloacal swabs were cultured for salmonella spp. annually. ten snakes survived the 5-yr period, with six of them remaining free of bony lesions. three snakes that had no bony lesions ...200212564525
comparison of sxt and r391, two conjugative integrating elements: definition of a genetic backbone for the mobilization of resistance determinants.the sxt element (sxt) is becoming an increasingly prevalent vector for the dissemination of antibiotic resistances in vibrio cholerae. sxt is a member of a larger family of elements, formerly defined as incj plasmids, that are self-transmissible by conjugation and integrate site-specifically into the host chromosome. comparison of the dna sequences of sxt and r391, an incj element from providencia rettgeri, indicate that these elements consist of a conserved backbone that mediates the regulation ...200212568332
production of glycosylated thermostable providencia rettgeri penicillin g amidase in pichia pastoris.penicillin g amidase from providencia rettgeri is a heterodimer of 92 kda. we have previously expressed the pr. rettgeri pac gene coding for this enzyme in saccharomyces cerevisiae, and now we report the expression and characterization in the methylotrophic yeast pichia pastoris. the recombinant catalytically active enzyme (rpac(pr)) was secreted from shake flask-grown p. pastoris cells into the medium at a level of approximately 0.18 u ml(-1). this yield of rpac(pr) was higher, by two orders of ...200212702330
improving the specific synthetic activity of a penicillin g acylase using dna family shuffling.penicillin g acylas (pga) of providencia rettgeri (atcc 25599) was evolved using a modified dna family shuffling method. the identity of pga genes from escherichia coli, kluyvera citrophila and providencia rettgeri ranges from 62.5% to 96.9%. the pga genes from above three species were recombined and shuffled to create interspecies pga gene fusion libraries. by substituting assembled chimaeras for corresponding region of petppga, different recombinants were constructed and expressed in e. coli j ...200312796820
hyperammonemic coma caused by providencia rettgeri infection in a child with prune belly syndrome.objective: to report a case of hyperammonemic encephalopathy in the absence of liver disease caused by a urinary tract infection with a previously unreported organism. design: case report. setting: pediatric intensive care unit. patient: critically ill child who presented with hyperammonemic coma. intervention: bladder drainage and antimicrobial therapy for the urinary tract infection. measurements: rapid resolution of the hyperammonemia. main results: rapid improvement in the patient's neurolog ...200112797879
prevalence of extended-spectrum beta-lactamases produced by nosocomial isolates of enterobacteriaceae in trakya university hospital, turkey.the prevalence of extended-spectrum beta-lactamase (esbl) production by 194 nosocomial isolates of enterobacteriacea recovered from 1995 to 1999 was investigated. the esbl production was determined by the double-disk synergy test and was confirmed by the e-test esbl strip. twenty-three isolates (21 klebsiella pneumoniae, one escherichia coli, one providencia rettgeri) were found as esbl-producers (11.8%). these isolates were also usually resistant to non-betalactam antibiotics. most of them cont ...200312901421
chlorhexidine as the probable cause of an increase in proteus rettgeri infections of the urinary tract. 196113763946
biochemical characteristics of lactose-fermenting proteus rettgeri from clinical specimens. 196213918554
synergistic action of kanamycin and tetracycline on proteus rettgeri infections of the urinary tract. 196213931384
lysogeny in proteus rettgeri and the hostrange of p. rettgeri and p. hauseri bacteriophages. 196314021947
effect of various concentrations of brilliant green and bile salts on salmonellae and other microorganisms.a study of the inhibitory effect of 24 different combinations of brilliant green and bile salts concentrations was conducted, using seven species of microorganisms capable of fermenting mannitol. the inhibitory effect of brilliant green decreased as the bile salts concentration was increased. staphylococcus aureus and proteus rettgeri were inhibited by all test media. escherichia coli was inhibited in all but two combinations of brilliant green and bile salts. aerobacter aerogenes generally foll ...196514264851
nosocomial urinary tract infections caused by proteus rettgeri. 196014428855
beta-lactamase stability of faropenem.faropenem (far) is an orally available member of the penem class unique among carbapenems and other available beta-lactams. this study compared far to cephalosporins and imipenem with respect to beta-lactamase (bla) stability and emergence of resistance to staphylococcus aureus and escherichia coli. bla stability was studied using enzyme preparations from sonicated/centrifuged 24-hour cultures of e. coli, enterobacter cloacae, proteus vulgaris, providencia rettgeri, klebsiella pneumoniae, s. aur ...200314504433
a new growth factor required by butyribacterium rettgeri. 195014774356
empyema due to proteus rettgeri report of a case with recovery. 195114861592
protease and phospholipase inhibition protect veneza zonata (hemiptera coreidae) against septicemia caused by parasite trypanosomatid 563dt.veneza zonata (hemiptera coreidae) is an insect which causes losses in several crops, and it is also an important vector of lower trypanosomatids. v. zonata specimens were collected on rural properties in londrina, state of paraná, southern brazil. inoculation of leptomonas 563dt into v. zonata hemocoel caused insect death within approximately 24 h, with large bacterial proliferation into their hemocoels. some bacteria which were found in the digestive tract of those insects, such as escherichia ...200414992855
cloning, overexpression, and characterization of a novel thermostable penicillin g acylase from achromobacter xylosoxidans: probing the molecular basis for its high thermostability.the gene encoding a novel penicillin g acylase (pga), designated pgaw, was cloned from achromobacter xylosoxidans and overexpressed in escherichia coli. the pgaw gene contains an open reading frame of 2586 nucleotides. the deduced protein sequence encoded by pgaw has about 50% amino acid identity to several well-characterized pgas, including those of providencia rettgeri, kluyvera cryocrescens, and escherichia coli. biochemical studies showed that the optimal temperature for this novel pga (pga6 ...200415128530
universal primer pcr with dgge for rapid detection of bacterial pathogens.a universal primer pcr (uppcr) combined with denaturing gradient gel electrophoresis (dgge) was evaluated as a method permitting the rapid detection of pathogens. the results show that this method is efficient at amplifying the conserved regions of bacterial 16s rrna genes with universal primers and can detect causative bacterial pathogens rapidly. six species of bacteria from fisheries (pseudomonas fluorescens, vibrio anguillarum, aeromonas hydrophila, vibrio fluvialis, providencia rettgeri and ...200415134888
per-1 production in a urinary isolate of providencia rettgeri.a providencia rettgeri strain resistant to extended-spectrum cephalosporins and intermediate to aztreonam was isolated from the urine of a patient hospitalized in the urology clinic of ssk educational hospital in ankara. clavulanic acid restored the activity of extended-spectrum cephalosporins, suggesting that the strain was harboring an extended-spectrum beta-lactamase. since the per-1 enzyme is widespread in turkey, and had been already detected in a related species such as proteus mirabilis, ...200415332708
metallo-beta-lactamase-producing gram-negative bacilli: laboratory-based surveillance in cooperation with 13 clinical laboratories in the kinki region of japan.a total of 19,753 strains of gram-negative rods collected during two 6-month periods (october 2000 to march 2001 and november 2001 to april 2002) from 13 clinical laboratories in the kinki region of japan were investigated for the production of metallo-beta-lactamases (mbls). mbls were detected in 96 (0.5%) of the 19,753 isolates by the broth microdilution method, the 2-mercaptopropionic acid inhibition test, and pcr and dna sequencing analyses. mbl-positive isolates were detected in 9 of 13 lab ...200415528723
cutaneous and pulmonary mycosis in green anacondas (euncectes murinus).two dead, captive green anacondas (eunectes murinus), including one male and one female, submitted for necropsy were in poor body condition, having multiple, scattered, dark red foci on the scales and mottled lungs. both snakes had severe mycotic dermatitis. in addition, the male snake had mycotic stomatitis, and the female snake had mycotic pneumonia. trichophyton sp., verticillium sp., and alternaria sp. were isolated from the dermal lesions. the pulmonary lesions were morphologically consiste ...200415732602
interaction of clindamycin and gentamicin in vitro.the minimal inhibitory concentrations of clindamycin and gentamicin alone and in combinations were determined by a microdilution method for 163 aerobic, facultative, and anaerobic clinical isolates. all 77 strains of staphylococcus aureus, diplococcus pneumoniae, streptococcus pyogenes, and anaerobic bacteria (except for three strains of clostridium) were inhibited by 1.6 mug or less of clindamycin per ml. gentamicin did not interfere with the activity of clindamycin within the range of concentr ...197415825309
oxalate-degrading providencia rettgeri isolated from human stools.oxalate-degrading bacteria are thought to metabolize intestinal oxalate and thus decrease the urinary excretion of oxalate by reducing its intestinal absorption.200515985073
expression and purification of penicillin g acylase enzymes from four different micro-organisms, and a comparative evaluation of their synthesis/hydrolysis ratios for cephalexin.several genes for the enzyme penicillin g acylase, as isolated from four different micro-organisms (alcaligenes facaelis, escherichia coli, kluyvera cryocrescens or providencia rettgeri) were modified at their carboxy-termini to include his-tag fusions, then were expressed from the plasmid pet-24a(+) in e. coli jm109(de3) cells. all fusion proteins were next purified to homogeneity in a single step by agar-based co-ida chromatography, and were then evaluated as catalysts for the synthesis of cep ...200616139515
influence of human ascitic fluid on the in vitro antibacterial activity of moxifloxacin.we investigated the in vitro influence of haf on the antibacterial activity of moxifloxacin against escherichia coli atcc 10798, escherichia coli k-12, proteus rettgeri (sanelli), staphylococcus aureus atcc 25923, staphylococcus aureus nctc 1808 and staphylococcus epidermidis atcc 12228. human ascitic fluid was obtained from 6 cirrhotic patients by paracentesis. the interaction effect was evaluated by the checkerboard technique. our results indicate the ability of human ascitic fluid to reduce m ...200516167519
metallo-beta-lactamase imp-1 in providencia rettgeri from two different hospitals in japan.in 2002, 495 indole-positive proteae strains were isolated from patients at 60 hospitals in japan. nine indole-positive proteae strains had reduced susceptibility to imipenem (mic > or = 8 microg ml(-1)) and were identified as providencia rettgeri by bd phoenix. eight of the nine prov. rettgeri isolates were confirmed as metallo-beta-lactamase producers by the double-disc synergy test. all the metallo-beta-lactamases were classified as imp-1 by pcr and dna sequence analysis. these bla(imp-1) gen ...200516192438
importance of providencia species as a major cause of travellers' diarrhoea.in this study the importance of providencia species as a cause of travellers' diarrhoea was examined using a selective medium developed by the authors. providencia species could easily be distinguished from other enteric pathogens by the colour of the colonies obtained. nine strains of providencia alcalifaciens, nine of providencia rettgeri and five of providencia stuartii were isolated from 130 specimens, representing a surprisingly high incidence of infection compared with other pathogens isol ...200516192440
high-level production and covalent immobilization of providencia rettgeri penicillin g acylase (pac) from recombinant pichia pastoris for the development of a novel and stable biocatalyst of industrial applicability.a complete, integrated process for the production of an innovative formulation of penicillin g acylase from providencia rettgeri(rpac(p.rett))of industrial applicability is reported. in order to improve the yield of rpac, the clone ln5.5, carrying four copies of pac gene integrated into the genome of pichia pastoris, was constructed. the proteinase activity of the recombinant strain was reduced by knockout of the pep4 gene encoding for proteinase a, resulting in an increased rpac(p.rett) activit ...200616259000
screening of antibiotics resistance to enterobacteriaceae, pseudomonas aeruginosa, and acinetobacter baumannii by an advanced expert system.the vitek2 advanced expert system (aes) gives information about the antibiotics-resistance mechanisms based on the biological validation derived from the vitek2 susceptibility result. in this study, we investigated whether or not this system correctly categorized the beta-lactamase resistance mechanism data derived from the vitek2 susceptibility result using the testing card, ast-n025, with enterobacteriaceae, pseudomonas aeruginosa, and acinetobacter baumannii. we used 131 strains, and their ph ...200516369735
antimicrobial activity of flavonoids from pelargonium radula (cav.) l'hérit.flavonoids from pelargonium radula (cav.) l'hérit were purified by column chromatography. two fractions were obtained: f1 (main flavonoid isoquercitrin) and f2 (main flavonoid rutin). in vitro antimicrobial activity of f1 and f2 were tested against eleven species of bacteria and eleven species of fungi. both fractions demonstrated strong inhibitory activity against staphylococcus aureus, proteus rettgeri, candida tropicalis and microsporum gypseum. staphylococcus sp. (coagulase-negative) and can ...200516375833
providencia vermicola sp. nov., isolated from infective juveniles of the entomopathogenic nematode steinernema thermophilum.in the course of isolating bacteria from infective juveniles of the entomopathogenic nematode steinernema thermophilum ganguly & singh, 2000, three isolates were obtained (op1t, op29 and vs3). on the basis of 16s rrna gene sequence analysis and riboprint patterns, these three strains were identical to each other but distinct from the type strains of the five recognized species of the genus providencia. based on biochemical and genomic analysis and supported by the low (<35 %) dna-dna relatedness ...200616514040
a serological study of proteus rettgeri and similar organisms. 194916561672
ocular infections caused by providencia rettgeri.to report a series of patients with ocular infections caused by the rarely described gram-negative bacterium, providencia rettgeri.200616797710
mosaic structure of p1658/97, a 125-kilobase plasmid harboring an active amplicon with the extended-spectrum beta-lactamase gene blashv-5.escherichia coli isolates recovered from patients during a clonal outbreak in a warsaw, poland, hospital in 1997 produced different levels of an extended-spectrum beta-lactamase (esbl) of the shv type. the beta-lactamase hyperproduction correlated with the multiplication of esbl gene copies within a plasmid. here, we present the complete nucleotide sequence of plasmid p1658/97 carried by the isolates recovered during the outbreak. the plasmid is 125,491 bp and shows a mosaic structure in which a ...200717220406
[purple urine bag syndrome: a case report].the authors report a case of dark purple urine in a woman with bowel obstruction and bilateral percutaneous nephrostomy urinary diversion for 30 years. this colour was due to the presence of high urinary concentrations of 3-indoxyl sulphate due to the enzymatic activity of providencia rettgeri. a favourable course was observed in response to antibiotics.200717634003
a serological study of proteus rettgeri and similar organisms. 194918113434
molecular identification of dominant microflora associated with 'hawaijar' - a traditional fermented soybean (glycine max (l.)) food of manipur, india.the dominant microorganisms in 'hawaijar', a traditional non-salted fermented soybean (glycine max (l.)) food of manipur, india, were isolated and identified by molecular techniques. bacillus spp. were the predominant microorganisms in 'hawaijar'. a total of 274 isolates were obtained from forty-one 'hawaijar' samples collected from household preparations and markets of valley districts of manipur. phenotypic grouping followed by amplified ribosomal dna restriction digestion analysis (ardra), pc ...200818281117
the evolutionary costs of immunological maintenance and deployment.the evolution of disease resistance and immune function may be limited if increased immunocompetence comes at the expense of other fitness-determining traits. both the maintenance of an immune system and the deployment of an immune response can be costly, and the observed costs may be evaluated as either physiological or evolutionary in origin. evolutionary costs of immunological maintenance are revealed as negative genetic correlations between immunocompetence and fitness in the absence of infe ...200818315877
[intestinal enterobacteria of the hibernating apis mellifera mellifera l. bees].dynamics of enterobacteria of normal intestinal microflora was studied in apis mellifera mellifera l. bees hibernating under snow in the western urals. the cell numbers (n) of the predominant species klebsiella oxytoca increased from 10-10(6) cfu/bee in november 2004 to 10(4)-10(7) cfu/bee in march 2005; its frequency of occurrence (p) increased from 92 to 100%. increase of providencia rettgeri (11.2004: n up to 10(6), p 25%; 03.2005: n 10(2)-10(6), p 80%) was accompanied by the substitution of ...200818683661
effect of triclosan on the formation of crystalline biofilms by mixed communities of urinary tract pathogens on urinary catheters.the crystalline bacterial biofilms that encrust foley catheters compromise the care of many elderly and disabled patients. the aim of this study was to examine whether the biocide triclosan can prevent encrustation by the mixed flora of uropathogens that commonly infect patients undergoing long-term catheterization. models of the catheterized bladder were inoculated with communities of organisms isolated from patients who were experiencing catheter blockage. the catheter retention balloons were ...200818719184
catheter-related urinary tract infection in patients suffering from spinal cord injuries.urinary tract infection is commoner in patients with spinal cord injuries because of incomplete bladder emptying and the use of catheters that can result in the introduction of bacteria into the bladder. 145 patients suffering from spinal cord injuries, admitted to the institute for physical medicine and rehabilitation, centre for paraplegia of the clinical centre of the university of sarajevo, were included. the patients were divided in three groups according to the method of bladder drainage: ...200919284388
biofilm evidence and the microbial diversity of horse wounds.evidence of biofilms in human chronic wounds are thought to be responsible for preventing healing in a timely manner. however, biofilm evidence in horse wounds has not yet been documented. consequently, this study aimed to determine whether biofilms could be detected in wounds, and to investigate the microbiology of chronic wounds in horses. prior to analysis, wound surfaces were irrigated with 5 ml of sterile saline to remove debris. all wounds were swabbed twice (1 cm2 area) using sterile cott ...200919295652
saccular lung cannulation in a ball python (python regius) to treat a tracheal obstruction.an adult male ball python (python regius) presented in a state of severe dyspnea characterized by open-mouth breathing and vertical positioning of the head and neck. the animal had copious discharge in the tracheal lumen acting as an obstruction. a tube was placed through the body wall into the caudal saccular aspect of the lung to allow the animal to breathe while treatment was initiated. the ball python's dyspnea immediately improved. diagnostics confirmed a bacterial respiratory infection wit ...200919368267
crystalline bacterial biofilm formation on urinary catheters by urease-producing urinary tract pathogens: a simple method of control.the problem of catheter encrustation stems from infection by urease-producing bacteria. these organisms generate ammonia from urea, elevate the ph of urine and cause crystals of calcium and magnesium phosphates to form in the urine and the biofilm that develops on the catheter. in this study, a laboratory model was used to compare the ability of 12 urease-positive species of urinary tract pathogens to encrust and block catheters. proteus mirabilis, proteus vulgaris and providencia rettgeri were ...200919556373
extended-spectrum beta-lactamases among enterobacteriaceae isolated in a public hospital in brazil.extended-spectrum beta-lactamases (esbl) in enterobacteria are recognized worldwide as a great hospital problem. in this study, 127 esbl-producing enterobacteriaceae isolated in one year from inpatients and outpatients at a public teaching hospital at são paulo, brazil, were submitted to analysis by pcr with specific primers for bla shv, bla tem and bla ctx-m genes. from the 127 isolates, 96 (75.6%) klebsiella pneumoniae, 12 (9.3%) escherichia coli, 8 (6.2%) morganella morganii, 3 (2.3%) proteus ...200919739000
the susceptibility of nosocomial pathogens to ceftazidime.the activities in vitro of ceftazidime and of cefuroxime have been compared against 201 strains of bacteria which had been isolated either from outbreaks of nosocomial infection or were typical of strains causing such outbreaks. ceftazidime was found to be about 1000 times more active against pseudomonas spp. and proteus vulgaris, and between 10 and 100 times more active against klebsiella pneumoniae, enterobacter spp., citrobacter freundii, serratia marcescens, proteus rettgeri, proteus morgani ...198119802966
automatic implantable cardioverter defibrillator pocket infection due to providencia rettgeri: a case report.coagulase-negative staphylococci and staphylococcus aureus are the commonest pathogens involved in infections of pacemaker-defibrillator systems. among causative gram-negative bacteria, infections due to klebsiella, serratia, pseudomonas, acinetobacter and other species have been reported. we report herein a unique case of an automatic implantable cardioverter defibrillator infection due to providencia rettgeri in a 65-year-old male who was admitted to our service with bacteremia and infection o ...200919918391
heterotrophic ammonium removal characteristics of an aerobic heterotrophic nitrifying-denitrifying bacterium, providencia rettgeri yl.bacterium providencia rettgeri yl was found to exhibit an unusual ability to heterotrophically nitrify and aerobically denitrify various concentrations of ammonium (nh4+-n). in order to further understand its removal ability, several experiments were conducted to identify the growth and ammonium removal response at different carbon to nitrogen (c/n) mass ratios, shaking speeds, temperatures, ammonium concentrations and to qualitatively verify the production of nitrogen gas using gas chromatograp ...200919999986
genotype and gene expression associations with immune function in drosophila.it is now well established that natural populations of drosophila melanogaster harbor substantial genetic variation associated with physiological measures of immune function. in no case, however, have intermediate measures of immune function, such as transcriptional activity of immune-related genes, been tested as mediators of phenotypic variation in immunity. in this study, we measured bacterial load sustained after infection of d. melanogaster with serratia marcescens, providencia rettgeri, en ...201020066029
heterotrophic nitrogen removal by providencia rettgeri strain yl.providencia rettgeri strain yl was found to be efficient in heterotrophic nitrogen removal under aerobic conditions. maximum removal of nh(4) (+)-n occurred under the conditions of ph 7 and supplemented with glucose as the carbon source. inorganic ions such as mg(2+), mn(2+), and zn(2+) largely influenced the growth and nitrogen removal efficiency. a quantitative detection of nitrogen gas by gas chromatography was conducted to evaluate the nitrogen removal by strain yl. from the nitrogen balance ...201020333440
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