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in vitro activity of 19 antimicrobial agents against 3513 nosocomial pathogens collected from 48 canadian medical centres. the canadian antimicrobial study group.antimicrobial resistance is a global concern. differentiation between susceptibility rates for nosocomial versus community pathogens is important epidemiologically because it impacts on the appropriate empirical selection of antimicrobial therapy for infected patients. we studied resistance rates for 3513 nosocomial pathogens from 48 canadian medical centres tested against 19 antimicrobial agents. the following are percent susceptibility for ceftazidime, ceftriaxone, ciprofloxacin, imipenem, net ...200010926444
isolations of enteric pathogens from synanthropic flies trapped in downtown kuala lumpur.four species of synanthropic flies were trapped in downtown kuala lumpur: chrysomya megacephala, chrysomya rufifacies, musca domestica, and musca sorbens. burkholderia pseudomallei, the organism causing melioidosis, was the dominant bacteria isolated from chrysomya megacephala. klebsiella oxytoca, commonly associated with nosocomial infections, was commonly isolated from chrysomya megacephala, musca domestica, and musca sorbens. aeromonas hydrophila, the bacteria causing gastroenteritis, was pre ...200010925800
multiple meningiomas, craniofacial hyperostosis and retinal abnormalities in proteus syndrome.because clinical evidence suggests that proteus syndrome may be caused by a somatic mutation during early development, resulting in mosaicism, the possible types of abnormalities and their clinical distributions are highly variable. here, we report on an unusual patient with proteus syndrome. manifestations included multiple meningiomas, polymicrogyria, and periventricular heterotopias. both eyes had epibulbar cystic lesions. the retina showed diffuse disorganization with nodular gliosis, retina ...200010925389
sequence diversity of intervening sequences (ivss) in the 23s ribosomal rna in salmonella spp.intervening sequences (ivss) occur sporadically in the rrl (ribosomal rna large) genes for 23s ribosomal rna (rrna) at helix-25 (base pair 550) and helix 45 (base pair 1170) in several bacterial genera, including salmonella, yersinia, proteus, and providencia, representing the enterobacteriaceae, but are missing from other genera such as escherichia. these sequences are transcribed, but later excised without re-ligation during rnaseiii processing of the rrna, resulting in fragmented 23s rrna. th ...200010925202
synthesis and antimicrobial activity of 4-carbethoxymethyl-2-[(alpha-haloacyl)amino] thiazoles and 5-nonsubstituted/substituted 2-[(4-carbethoxymethylthiazol-2-yl)imino]-4-thiazolidinones.4-carbethoxymethyl-2-[(chloroacetyl/alpha-chloropropionyl/al pha- bromobutyryl/alpha-chloro-(alpha-phenylacetyl)amino]thiazoles (i-iv) were synthesized by reacting 4-carbethoxymethyl-2-aminothiazole with chloroacetyl chloride, alpha-chloropropionyl chloride, alpha-bromobutyryl bromide and alpha-chloro-alpha-phenylacetyl chloride, respectively. furthermore, i-iv were refluxed with ammonium thiocyanate to give 2-[(4-carbethoxymethylthiazol-2-yl)imino]-4-thiazolidinones (v-viii). v was refluxed wit ...200010918953
characteristics of neonatal bacterial meningitis in a teaching hospital in taiwan from 1984-1997.during the period from 1984 to 1997, 85 bacterial meningitis neonates with positive cerebrospinal fluid cultures were treated. the ages of these patients ranged from 1 to 28 days. the male to female ratio was 1.7 to 1. the most common causative agent was group b beta-hemolytic streptococci (gbs, 31.8%), followed by escherichia coli (20%), proteus mirabilis (7.1%), enterobacter cloacae (5.9%), other streptococci excluding streptococcus pneumoniae (5.9%), chryseobacterium meningosepticum (5.9%), e ...200010917879
culture independent and rapid identification of bacterial pathogens in necrotising fasciitis and streptococcal toxic shock syndrome by fluorescence in situ hybridisation.fluorescence in situ hybridisation (fish) targeted to ribosomal rna is well established for studies in environmental microbiology. initial applications of this technique in the field of medical microbiology showed that fish is also a suitable means for the rapid, reliable and cultivation-independent identification of bacterial pathogens. in particular, for infectious diseases that follow a fulminant live-threatening course, such as sepsis or necrotising fasciitis (nf), a fast and reliable detect ...200010917153
waste water bacterial isolates resistant to heavy metals and antibiotics.sewage water of casablanca, an industrial city in morocco, was studied for microorganisms resistant to heavy metals. isolates were purified and collected on agar slants to be screened for resistance to heavy metals, including mercury in vitro. the strains that showed high resistance to heavy metals were also studied for their resistance to antibiotics and aromatic hydrocarbons. results indicated that the strains most resistant to all tested products belonged to ps. fluorescens, ps. aeruginosa, k ...200010915198
[bacteriologic and clinical analysis of nosocomial infections in patients from the intensive care unit].the aim of this study is to evaluate what pathogens are mainly responsible for infection among all hospitalised at our itu patients, to define the influence of antibiotic use on the aetiology of nosocomial infection. the research was conducted on a six-bedded surgical itu in a 700-bed teaching hospital from january 1995 till june 1996. in august 1995 we changed infection control protocols (more stress on: handwashing with antiseptic soaps and routine microbiological culture for early prediction ...199910909491
[enterobacterial native valve endocarditis in the intensive care unit: report of two cases]. 200010909160
elisa for bovine interleukin-1 receptor antagonist and its application to mastitic sera and whey.a sandwich elisa for the bovine il-1 receptor antagonist (bil-1ra) was developed using recombinant (r) bil-1ra produced by escherichia coli, anti-rbil-1ra rabbit igg, its biotinylated one and avidin-peroxidase. this elisa system enabled detection of rbil-1ra at a concentration of more than 2 ng/ml. this elisa was applied to quantitation of bil-1ra in sera and whey of mastitic and healthy cows. the results indicate that although il-1ra levels in healthy and mastitic sera and whey were comparable, ...200010907698
specific mucosal immunity in the pathophysiology of bacterial prostatitis in a rat model.mucosal immunity was established in the rat prostate by stimulating the common mucosal system through serosal exposure of formalin-killed escherichia coli. immunized but not sham-immunized rats developed bacterial specific igg and iga in prostatic fluid, and iga in urine. immunized (n = 21) and sham-immunized control rats (n = 30) were challenged by transurethral injection of e. coli into the prostate ducts. mortality, gross and microscopic pathology, tissue bacterial counts, bacterial associate ...199910907422
characterization of trans-splicing in euglenoids.we have looked for trans-splicing of nuclear mrnas in several euglenoid species. in cyclidiopsis acus, phacus curvicauda, rhabdomonas costata and menoidium pellucidum we showed that several premrnas chosen at random are matured by a transsplicing process: we identified sl-rna genes whose 5' ends (sls for spliced leader-sequences) were transferred to the 5' extremities of mrnas. the sl-rna genes are located on repeated dna fragments which also encode 5s rrna in p. curvicauda and c. acus. the pote ...200010905424
pvuii endonuclease contains two calcium ions in active sites.restriction endonucleases differ in their use of metal cofactors despite having remarkably similar folds for their catalytic regions. to explore this, we have characterized the interaction of endonuclease pvuii with the catalytically incompetent cation ca(2+). the structure of a glutaraldehyde-crosslinked crystal of the endonuclease pvuii-dna complex, determined in the presence of ca(2+) at a ph of approximately 6.5, supports a two-metal mechanism of dna cleavage by pvuii. the first ca(2+) posit ...200010903853
fusion of the tissue factor extracellular domain to a tumour stroma specific single-chain fragment variable antibody results in an antigen-specific coagulation-promoting molecule.solid tumours growing beyond a size of 1-2 mm in diameter induce supporting connective tissue structures, the tumour stroma, comprising activated fibroblasts and newly formed blood vessels, embedded in an extracellular matrix. the selective destruction of this tissue or the inhibition of its function (e.g. tumour neoangiogenesis) may result in the destruction of tumour nodules, thus providing novel opportunities for tumour therapy. our approach aims at an antibody-mediated induction of coagulati ...200010903142
the structure of the core part of proteus penneri strain 16 lipopolysaccharide.the structure of the carbohydrate backbone of the lipid a-core region of the lipopolysaccharide (lps) from proteus penneri strain 16 was determined using nmr and chemical analysis of the core oligosaccharide, obtained by mild acid hydrolysis of the lps, and of the products of alkaline deacylation of the lps: formula [see text]. incomplete substitution is indicated by bold italics. all sugars are in the pyranose form, alpha-hep is the residue of l-glycero-alpha-d-manno-hep, alpha-dd-hep is the re ...200010903028
[urine culture analysis in children with urinary tract infections treated at pediatric clinical department in bytom in the years 1998-1999].sensitivity of bacterial strains causing urinary tract infections was analysed. high sensitivity of bacteria to nitrofurantoin, guinolones and cephalosporines--mainly of the iii generation, as well as minute sensitivity to penicillins were demonstrated.200010897628
health evaluation of arctic fox (alopex lagopus) cubs in sweden.hematologic, serum biochemistry, and serum cortisol reference ranges were established and tonsil/rectal bacterial and fecal parasite examinations were performed on 21 wild arctic fox (alopex lagopus) cubs during july 1996. several of the hematologic and serum biochemistry values fell within normal ranges for other wild canids or domestic dogs of the same age class. serum alanine transaminase and creatine phosphokinase values were significantly higher in the youngest cubs. proteus vulgaris and es ...200010884121
development of an operational substrate for zapa, a metalloprotease secreted by the bacterium proteus mirabilis.the protease zapa, secreted by proteus mirabilis, has been considered to be a virulence factor of this opportunistic bacterium. the control of its expression requires the use of an appropriate methodology, which until now has not been developed. the present study focused on the replacement of azocasein with fluorogenic substrates, and on the definition of enzyme specificity. eight fluorogenic substrates were tested, and the peptide abz-ala-phe-arg-ser-ala-ala-gln-eddnp was found to be the most c ...200010881051
lupene-type triterpenes from periploca aphylla.two new lupane derivatives, 3beta,6alpha-dihydroxylup-20(29)-ene (1) and 6alpha-hydroxylup-20(29)-en-3beta-octadecanoate (2), have been isolated from the stems of periploca aphylla, in addition to beta-sitosterol and lupeol. the structures of 1 and 2 were determined by spectral and chemical methods. compound 1 showed strong inhibition of alpha-glucosidase type vi and a moderate antibacterial activity.200010869229
influence of clinical history on airways bacterial colonization in subjects with chronic tracheostomy.patients with chronic tracheostomy are subject to significant bacterial colonization of the airways, a risk factor for respiratory infections. the aim of our study was to verify whether bacterial colonization and humoral immune response in the airways can be influenced by the disease which led to chronic respiratory failure and tracheostomy. thirty-nine clinically stable outpatients with chronic tracheostomy were considered: 24 were affected by chronic obstructive pulmonary disease (copd) (mean ...200010868706
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (1998). ii. background of patients].clinical background was investigated on 449 patients with urinary tract infections (utis) from whom 591 bacterial strains were isolated in 9 hospitals during the period from june, 1998 through may, 1999. about distribution of age and sex of patients and type of infections, among males, patients less than 50 years old were few, and uncomplicated utis without indwelling catheters was most frequent. among females, patients less than 20 years old were few, and uncomplicated was most frequent. escher ...200010868301
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (1998). i. susceptibility distribution].the frequencies of bacterial isolation and susceptibilities to antimicrobial agents were investigated on 538 bacterial strains isolated from patients with urinary tract infections (utis) in 9 hospitals during the period of june 1998 to may 1999. of the above bacterial isolates, gram-positive bacteria accounted for 30.3% and gram-negative bacteria accounted for 69.7%. susceptibilities of several isolated bacteria to antimicrobial agents were as follows; against enterococcus faecalis isolated from ...200010868300
cefditoren activity against nearly 1000 non-fastidious bacterial isolates and the development of in vitro susceptibility test methods.cefditoren (formerly me-1206) is an investigational, orally administered cephalosporin ester with bactericidal activity against many gram-positive and -negative organisms. cefditoren potency against nearly 1000 non-fastidious species was determined by national committee for clinical laboratory standards (nccls) reference broth microdilution and standardized disk diffusion methods. against staphylococci, usable cefditoren activity was completely correlated with oxacillin with respect to potency a ...200010863109
ocular manifestations in proteus syndrome.we report on the ocular manifestations of a proteus syndrome patient. several of the manifestations are due to severe maldevelopment and malfunction of the neuroretina including strabismus, nystagmus, high myopia, and retinal pigmentary abnormalities. in reviewing the literature, strabismus and epibulbar tumors were recorded most commonly. some articles about presumed proteus syndrome are spurious; these have not been included here. also, because of anecdotal and nonsystematic study of the eye a ...200010861666
respiratory pathogen colonization of the dental plaque of institutionalized elders.although it has been established that aspiration of pharyngeal bacteria is the major route of infection in the development of nosocomial pneumonia, colonization of the pharyngeal mucosa by respiratory pathogens has been shown to be a transient phenomenon. it has been suggested that the dental plaque may constitute an additional, possibly more stable, reservoir of respiratory pathogens. the purpose of this study was to assess the prevalence of oral colonization by potential respiratory pathogens ...199910860077
in vitro activity of rifaximin, metronidazole and vancomycin against clostridium difficile and the rate of selection of spontaneously resistant mutants against representative anaerobic and aerobic bacteria, including ammonia-producing species.rifaximin is a rifamycin derivative characterized by a wide antibacterial activity. this drug is neither absorbed by the gastrointestinal tract nor inactivated by gastric juices, and exerts its action entirely within the intestinal lumen.200010859431
a novel ctx-m beta-lactamase (ctx-m-8) in cefotaxime-resistant enterobacteriaceae isolated in brazil.to estimate the diversity of extended-spectrum beta-lactamases in brazil, 18 strains from different species of the family enterobacteriaceae exhibiting a positive double-disk synergy test were collected by a clinical laboratory from several hospitals in rio de janeiro, brazil, in 1996 and 1997. four strains (proteus mirabilis, enterobacter cloacae, enterobacter aerogenes, and citrobacter amalonaticus) hybridized with a 550-bp ctx-m probe. the p. mirabilis strain produced a ctx-m-2 enzyme. the e. ...200010858358
prevalence of beta-lactamases among 1,072 clinical strains of proteus mirabilis: a 2-year survey in a french hospital.beta-lactam resistance was studied in 1,072 consecutive p. mirabilis clinical strains isolated at the clermont-ferrand teaching hospital between april 1996 and march 1998. the frequency of amoxicillin resistance was 48.5%. among the 520 amoxicillin-resistant isolates, three resistance phenotypes were detected: penicillinase (407 strains [78.3%]), extended-spectrum beta-lactamase (74 strains [14. 2%]), and inhibitor resistance (39 strains [7.5%]). the penicillinase phenotype isolates were divided ...200010858357
a new rapid automated method for the detection of listeria from environmental swabs and sponges.many food and meat processors test environmental swabs and sponges to confirm the absence of listeria spp. spectral pattern changes in a liquid growth medium, resulting from esculin hydrolysis by listeria in contaminated swabs and sponges, were automatically monitored by the biosys instrument in a semifluid layer (sfl). the blackening of sfl in modified mox broth resulted in sharply declining curves, which were easily detected by the instrument. the instrument detected all nine strains of lister ...200010857550
[enterobacterial sensitivity to beta-lactam antibiotics].the susceptibility to betalactams of 868 enteric bacteria isolated from the patients at the hospital was studied. the isolated pathogens included: e. coli (549), klebsiella sp. (195), serratia sp. (124). ampicillin and cefazoline demonstrated the lowest activity. cefotaxime, ceftazidime and imipenem were active against 90 per cent of isolates. among e. coli isolates the susceptibility to the above mentioned drugs was the following: 95.1, 96.9, 99.3 per cent, among klebsiella sp.--89.7, 88.7, 97. ...200010851647
[accuracy of antibiograms performed directly from urine incubation bottles of the sebac system for diagnosing bacteriuria]. 200010846244
epidemiology of infectious keratoconjunctivitis in cattle in south-east ethiopia.the aim of the study was to determine the prevalence of infectious keratoconjunctivitis on cross-breed dairy farms and to evaluate factors that influence its distribution. a total of 11 dairy farms and one cattle cross-breeding farm in different parts of arsi region, south-east ethiopia were clinically and bacteriologically investigated from february 1989 to december 1990. out of 5221 local zebu and cross-breed dairy animals, 110 were found to have different degrees of infectious bovine keratoco ...200010842466
antimicrobial resistance in enterobacteriaceae in brooklyn, ny: epidemiology and relation to antibiotic usage patterns.in november 1997, all enterobacteriaceae isolated at 15 hospitals in brooklyn were collected. extended-spectrum beta-lactamases (esbls) were present in 44% of 409 klebsiella pneumoniae isolates. six isolates had reduced susceptibility to carbapenems, including two that were not susceptible to any of the antibiotics tested. pulsed field gel electrophoresis revealed a commonality of resistant isolates within and between hospitals. the occurrence of esblcontaining isolates was associated with cepha ...200010837447
use of pcr with universal primers and restriction endonuclease digestions for detection and identification of common bacterial pathogens in cerebrospinal fluid.we have designed a universal pcr capable of amplifying a portion of the 16s rrna gene of eubacteria, including staphylococcus aureus, staphylococcus epidermidis, streptococcus pyogenes, streptococcus agalactiae, streptococcus pneumoniae, enterococcus faecium, enterococcus faecalis, mycobacterium tuberculosis, legionella pneumophila, escherichia coli, klebsiella pneumoniae, serratia marcescens, enterobacter cloacae, pseudomonas aeruginosa, acinetobacter baumannii, proteus mirabilis, haemophilus i ...200010834956
in vitro activity of difloxacin against canine bacterial isolates.the in vitro activity of difloxacin against canine bacterial isolates from clinical cases was studied in the united states and the netherlands. minimal inhibitory concentrations (mic), the postantibiotic effect, the effect of ph on antimicrobial activity, and the bacterial killing rate tests were determined according to standard techniques. the mics of american and dutch isolates agreed in general. the mics of the american gram-negative isolates ranged from 0.06 to 2.0 microg/ml, and the mics of ...200010826834
medicine and the arts. the elephant man. 200010824768
comparative efficacy of gemifloxacin in experimental models of pyelonephritis and wound infection.gemifloxacin (sb-265805) is a potent, novel fluoroquinolone with broad-spectrum antimicrobial activity. in this study, the efficacy of gemifloxacin was studied in experimental models of gram-negative pyelonephritis (caused by escherichia coli or proteus mirabilis) and gram-positive wound infection resulting from streptococcus pyogenes, staphylococcus epidermidis or staphylococcus aureus. gemifloxacin activity against these pathogens was compared with those of amoxycillin-clavulanate, ciprofloxac ...200010824038
in vitro activity of gemifloxacin against a broad range of recent clinical isolates from the usa.the antibacterial potencies of gemifloxacin (sb-265805) and 13 comparator compounds were determined by broth microdilution against a panel of 645 gram-positive and 995 gram-negative organisms collected from various usa sites. time-kill studies were performed and postantibiotic effect (pae) was determined for several organisms using trovafloxacin and ciprofloxacin as comparator compounds. based on mic(90)s, gemifloxacin was the most potent compound tested against gram-positive isolates: streptoco ...200010824027
synthesis and antibacterial activity of some new non-proteinogenic amino acids containing thiazole residues.some new thioamides and thiazoles have been synthesized using canavanine, s-cysteine, homo-s-cysteinesulfonamides and their n-omega aminoethylated derivatives as adducts in order to investigate the structure-antimicrobial activity relationships. the compounds showed substantial antibacterial activity in vitro against various gram-positive (staphylococcus aureus, bacillus cereus etc.) and gram-negative (escherichia coli, proteus vulgaris etc.) bacteria. these findings indicate that the presence o ...200010817409
[frequency and antibiotic susceptibility of urinary pathogens isolated in the microbiology and virology laboratory of the careggi general hospital in the period july-december 1996].the aim of the study was to evaluate the isolation frequency and antibiotic susceptibility of pathogens isolated form urinocoltures.199910812907
paronychia and excess granulation tissue of the toes and finger in a patient treated with indinavir. 200010809885
alpha1-microglobulin as a marker of proximal tubular damage in urinary tract infection in children.the urinary alpha1-microglobulin (alpha1-m) as a marker of proximal tubular damage was measured in 86 children, aged 3/12 to 12 years, with a diagnosis of urinary tract infection (uti) and fever. all patients had normal glomerular filtration rates (gfr). they were divided into 2 groups: a: with uti and etiological factor e. coli, b: with uti and etiological factor proteus sp. similar measurements of alpha1-m were obtained for a control group of healthy children. an increased mean level serum alp ...200010809416
the anti-microbial activity of maggot secretions: results of a preliminary study.the ability of larval secretions to kill or prevent the growth of a range of potentially pathogenic bacteria was investigated in a preliminary laboratory study. marked anti-microbial activity was detected against streptococcus a and b and staphylococcus aureus. some activity was also detected against pseudomonas sp. and a clinical isolate of a resistant strain of s. aureus (mrsa). no evidence of inhibition was recorded against enterococcus or the gram-negative bacteria escherichia coli and prote ...199910808842
pathological case of the month. proteus syndrome. 200010807308
[drug resistance and proticinogenic types of proteus mirabilis isolated from urinary tract infections].proteus mirabilis strains (88 isolates) from hospitalised patients with urinary tract infection were tested for antibiotic susceptibility, esbl production and their ability to produce proticin or on their susceptibility to proticin. antibiotic susceptibility test was performed by standard disc diffusion method according to nccls. proticin typing was made by the standard strain set from the b. w. senior collection. most (59%) strains belonged to esbl producers and were more resistant to antibioti ...199910803261
[descriptive study of infectious ear disease in relation to summer].a descriptive study was made of infectious ear disease (including diffuse otitis externa, otomycosis, acute-on-chronic otitis media, and superinfection of a radical mastoidectomy cavity) in relation to changes of weather and habits in summer. during the months of june, july, and august 1996, 179 patients were evaluated in the emergency room of the alicante general university hospital, spain. average patient age at presentation was 30.52 (+/- 20.08) years and 56% were men. the most frequent disea ...200010799927
gender differences in response to the multitest cmi skin test in the general population.previous studies of gender differences in response to the multitest cmi skin test have produced conflicting results.200010795654
iron status of libyan infants with urinary tract infection.serum iron, serum total iron binding capacity and tranferrin saturation levels were measured in 45 infants, of whom 19 had urinary tract infections confirmed by positive suprapubic aspiration. the control group comprised 26 healthy infants with negative results. mean serum iron and transferrin saturation values were significantly lower in infants with urinary tract infection compared with the control group (p < 0.01 and p < 0.001 respectively), especially if the cultured organism was escherichia ...199910793812
[a probiotic as an antagonist of bacterial translocation in experimental pancreatitis].infection is the most common cause of death in acute pancreatitis. earlier studies have demonstrated that early enteral nutrition decreases microbial translocation, upregulates the immune function and reduces septic complications and mortality. lactobacillus plantarum (lp) has been shown to be effective in reducing egress of endotoxin and microbial strain that showed very high adherence power to gut mucosa. we adopted a model of acute pancreatitis induced by isolation and ligation of biliopancre ...199910793768
treatment of sanitary-important bacteria by bacteriocin substance v24 in cattle dung water.quantification of sanitary-important bacteria (e.g. enterobacteriaceae), as well as indicators of environmental contamination, was assessed in samples of cattle dung from 25 cattle farms in 15 north-eastern slovakia districts. the inhibitory effect of crude bacteriocin extract cbe v24 from enterococcus faecalis v24 against listeria monocytogenes ohio and yersinia enterocolitica ye85 was examined in cattle dung water with the aim of finding a new way of eliminating the health risk of the animal s ...200010792671
paediatric anatrophic nephrolithotomy; stone clearance - at what price?to evaluate the functional outcome of anatrophic nephrolithotomy in children.200010792169
occurrence and detection of ampc beta-lactamases among escherichia coli, klebsiella pneumoniae, and proteus mirabilis isolates at a veterans medical center.ampc beta-lactamases are cephalosporinases that confer resistance to a wide variety of beta-lactam drugs and that may thereby create serious therapeutic problems. although reported with increasing frequency, the true rate of occurrence of ampc beta-lactamases in escherichia coli, klebsiella pneumoniae, and proteus mirabilis remains unknown. we tested a total of 1,286 consecutive, nonrepeat isolates of these three species and found that, overall, 45 (3.5%) yielded a cefoxitin zone diameter less t ...200010790101
antibiotic prophylaxis in children with relapsing urinary tract infections: review.recurrent urinary tract infections (utis) are observed in 30-50% of children after the first uti. of these, approximately 90% occur within 3 months of the initial episode. the basic aim of antibiotic prophylaxis in children with malformative uropathy and/or recurrent utis, is to reduce the frequency of utis. the bacteria most frequently responsible for uti are gram-negative organisms, with escherichia coli accounting for 80% of urinary tract pathogens. in children with recurrent utis and in thos ...200010789549
[laboratory diagnosis and serologic course in patients with tularemia].tularemia was practically unknown in spain until the end of 1997, when an epidemic outbreak was declared. this paper presents the data on microbiological diagnosis of 55 patients who suffered from tularemia.200010786358
frequency and clinical characteristics of mycoplasma urinary tract infections in the early post-transplantation period in renal allograft patients.using a highly sensitive and specific polymerase chain reaction (pcr) protocol, we studied 36 renal allograft recipients and 30 healthy controls. midstream urine samples were analyzed using routine microbiological methods and the mycoplasma ist (biomerieux, france). mycoplasma infections of the upper urinary tract were found in 9 patients. in three of them e.coli and proteus were also present. we discuss the clinical characteristics of mycoplasma infections in the early post-transplantation peri ...199910786206
[detection of extended spectrum beta-lactamases producing enterobacteriaceae in feces].a study was made of 366 feces for detection of extended spectrum beta-lactamases producing enterobacteriaceae from feces. the selective agar was used for modified drigalski agar (eiken chemical co., ltd) with 2 micrograms/ml cefotaxime (esbl screen agar). 92 strains of enterobacteriaceae, 41 escherichia coli, 15 citrobacter freundii, 13 enterobacter cloacae, 11 klebsiella pneumoniae, and other 12, were isolated from esbl screen agar. and, r-plasmid that were selected by 2 micrograms/ml cefotaxim ...200010783580
structure of the o-specific polysaccharide of proteus mirabilis o11, another proteus o-antigen containing an amide of d-galacturonic acid with l-threonine.the o-specific polysaccharide of proteus mirabilis o11 was studied by sugar analysis, smith degradation, 1h and 13c nmr spectroscopy, including two-dimensional cosy, tocsy, noesy, and 1h-detected 1h, 13c hmqc experiments. the following structure of a pentasaccharide repeating unit of the polysaccharide was established: [formual: see text] where d-gala6lthr is n-(d-galacturonoyl)-l-threonine. elisa with anti-p. mirabilis o11 serum showed that d-gala6lthr is of minor importance for manifesting the ...200010782289
assessment of the potential for microbial resistance to topical use of multiple antimicrobial agents.the goal of this study was to reduce the likelihood of the generation and/or persistence of bacterial resistance to some antimicrobial components contained in a topical antimicrobial mixture (neomycin, polymyxin b, mupirocin and ciprofloxacin) for use with cultured skin grafts, by substitution of alternative antimicrobials, specifically fusidic acid for mupirocin and ofloxacin for ciprofloxacin. the alternative agents failed to serve that purpose. however, with the exception of specific genera o ...199910781215
frequency and time course of pancreatic and extrapancreatic bacterial infection in experimental acute pancreatitis in rats.infectious complications in severe pancreatitis are the main factors determining clinical course and outcome. the taurocholate model for acute necrotizing pancreatitis was evaluated for frequency and time course of pancreatic and extrapancreatic bacterial infection.200010776434
[hypercalcemia due to septic bone metastasis]. 200010776416
epidemiology and antibiotic resistance of gram-negative urinary pathogens in pediatric patients.in order to determine the etiological agents and the rate of resistance to various antibiotics, 209 consecutive gram-negative bacteria isolated from children admitted to hacettepe university children's hospital with urinary tract infections were investigated over a three-month period. of these, 46 (22%) were nosocomial isolates. the most frequently isolated organism was e.coli (n: 141) followed by klebsiella spp. (39), proteus spp. (19), pseudomonas spp. (8) and enterobacter spp. (2). in vitro s ...199910770674
[primary bacterial aortitis].primary bacterial aortitis represents a rare disease with a high lethality. from june 1997 to april 1999 5 patients with an abdominal aortic infection were treated by resection of the infected aorta and in-situ reconstruction or by extra-anatomic bypass. there was no treatment in one case because of the infaust prognosis. 3 patients survived, one with a paraparesis as a result of spinal ischemia. on the basis of our patients the pathogenesis, clinical symptoms with diagnosis and the therapeutic ...200010769448
antimicrobial resistance among urinary tract infection (uti) isolates in europe: results from the sentry antimicrobial surveillance program 1997.the sentry antimicrobial surveillance program was established to monitor the occurrence and antimicrobial susceptibility of bacterial pathogens via an international network of sentinel hospitals. twenty european hospitals referred a total of 887 urinary tract infection (uti) isolates to the european sentry reference laboratory during the period october-december 1997. ninety percent of the referred species were represented by escherichia coli (52%), enterococcus spp. (12%), klebsiella spp. (7%), ...200010768473
intracranial complications of acute mastoiditis.oral antibiotic use may have changed the incidence and microbiology of otitic intracranial complications. we reviewed cases of acute mastoiditis to document: (1) incidence of intracranial complications; (2) risk factors; and (3) identify pathologic organisms.200010767461
aerobic and anaerobic microbiology of wound infection following spinal fusion in children.to study the aerobic and anaerobic microbiology of wound infections following spinal fusion in children.200010765134
h-ns is a repressor of the proteus mirabilis urease transcriptional activator gene urer.expression of proteus mirabilis urease is governed by urer, an arac-like positive transcriptional activator. a poly(a) tract nucleotide sequence, consisting of a(6)ta(2)ca(2)tggta(5)ga(6)tga(5), is located 16 bp upstream of the sigma(70)-like urer promoter p2. since poly(a) tracts of dna serve as binding sites for the gene repressor histone-like nucleoid structuring protein (h-ns), we measured beta-galactosidase activity of wild-type escherichia coli mc4100 (h-ns(+)) and its isogenic derivative ...200010762273
bacterial contamination of bath-water from spinal cord lesioned patients with pressure ulcers exercising in the water.to evaluate the bacterial contamination of the water from the microflora of pressure ulcers in para- and tetraplegic patients, when they were exercising in water at 36 degrees c for half an hour.200010762183
structural analysis of the core region of lipopolysaccharides from proteus mirabilis serotypes o6, o48 and o57.the structure of lipid a core region of the lipopolysaccharides (lps) from proteus mirabilis serotypes o6, o57 and o48 was determined using nmr, ms and chemical analysis of the oligosaccharides, obtained by mild acid hydrolysis, alkaline deacylation, and deamination of lps: [see text for structure]. incomplete substitutions are indicated by bold italic type. all sugars are present in pyranose form, alpha-hep is the residue of l-glycero-alpha-d-manno-hep, alpha-dd-hep is the residue of d-glycero- ...200010759870
hla molecules, bacteria and autoimmunity.it has been well established that many diseases are linked to hla antigens. two of the most interesting hla associations may provide some insight into the pathogenesis of rheumatic inflammatory conditions. in ankylosing spondylitis (as), 96% of patients possess hla-b27, whilst the frequency of this marker in the general population is c. 8%. in rheumatoid arthritis (ra), >90% of patients possess either hla-dr1 or some subtypes of hla-dr4, whilst the frequency of this marker in the general populat ...200010755623
[microbiological study of gallbladder bile in a high risk zone for gallbladder cancer].gallbladder cancer is frequent in chile and there is sparse information about the association between this type of cancer and the presence of bacteria in the gallbladder bile.199910752267
[production of the therapeutic and prophylactic preparation enterobifidin on the basis of bifidobacterium adolescentis mc-42].production of enterobifidin comprises preparation of culture media, reparation of lyophilized bifidobacterium adolescentis ms-42 culture, preparation of starters, cultivation of bacteria in fermenters, biomass conservation, and its biological control. the preparation contains physiologically active bifidobacterium cells with high activities of growth (mu = 0.7 h-1, g = 1.0 h) and acid formation (titratable acidity is approximately 120-140 degrees t; acetate concentration, 0.50-0.75%; and lactate ...200010752094
in vitro susceptibility of gram-negative bacterial isolates to chlorhexidine gluconate.to investigate the susceptibility of clinical isolates of gram-negative bacteria to chlorhexidine gluconate.199910750501
germline and germline mosaic pten mutations associated with a proteus-like syndrome of hemihypertrophy, lower limb asymmetry, arteriovenous malformations and lipomatosis.germline pten mutations cause cowden syndrome (cs) and bannayan-riley-ruvalcaba syndrome (brr), two hamartoma-tumour syndromes, and somatic pten alterations have been shown to participate, to a greater or lesser extent, in a wide variety of sporadic neoplasia. pten is a tumour suppressor and dual-specificity phosphatase which affects apoptosis via its lipid phosphatase activity in the phosphoinositol-3-kinase and akt pathway as well as inhibiting cell spreading via the focal adhesion kinase path ...200010749983
clinical relevance of proteus mirabilis in hospital patients: a two year survey.a retrospective study was performed on 1072 non-duplicate isolates of proteus mirabilis, taken in the period april 1996 to march 1998, and on 100 patient charts randomly selected during the same period. p. mirabilis isolates accounted for 7.7% of enterobacteriaceae. the isolates were predominantly from urine (70.2%); of the total, 38.0% were penicillinase-producing isolates, 6.9% were extended-spectrum beta-lactamase (esbl)-producing isolates and 3.6% produced inhibitor-resistant beta-lactamase ...200010747835
in vitro antibacterial spectrum of a new broad-spectrum 8-methoxy fluoroquinolone, gatifloxacin.the in vitro antibacterial spectrum of gatifloxacin was compared with those of ciprofloxacin and ofloxacin. gatifloxacin was two- to four-fold more potent than comparator quinolones against staphylococci, streptococci, pneumococci and enterococci (gatifloxacin mic90s, < or =1 mg/l, except 4 mg/l against methicillin-resistant staphylococcus aureus and enterococcus faecium). gatifloxacin was two-fold less potent than ciprofloxacin, and the same as or two-fold more potent than ofloxacin against ent ...200010747819
antibiotic susceptibility of bacterial strains isolated from patients with community-acquired urinary tract infections in france. multicentre study group.the aim of this study was to determine the distribution and antibiotic susceptibility patterns of bacterial strains isolated from adults with community-acquired urinary tract infections (uti) in france. from december 1996 to march 1997, each of 15 private laboratories in france consecutively collected about 80 non-duplicate strains isolated from adult outpatients with uti, including patients receiving care at home, and tested their susceptibility by the disk diffusion test. a total of 1160 strai ...200010746497
antimicrobial activity and spectrum of the new glycylcycline, gar-936 tested against 1,203 recent clinical bacterial isolates.the in vitro activity of gar-936, a new semisynthetic glycylcycline, was evaluated in comparison with two tetracyclines and several other antimicrobial agents. a total of 1,203 recent clinical isolates were tested by reference broth or agar dilution methods. among the members of the family enterobacteriaceae, gar-936 was generally two- to four-fold more active than minocycline, and two- to 16-fold more active than tetracycline. all enteric bacilli mic90 results were < or = 4 microg/ml; the excep ...200010744364
infection and rheumatoid arthritis: guilt by association? 200010743788
is rheumatoid arthritis a form of reactive arthritis? 200010743787
urinary tract infection in diabetic patients.the aim of the study was to compare the epidemiological, microbiological and clinical features of diabetic patients with urinary tract infection (uti) to those of nondiabetic ones.199910743697
proteus syndrome: mri characteristics of plantar cerebriform hyperplasia.proteus syndrome is a rare congenital hamartomatous syndrome with a variety of abnormalities. it shares many features with other congenital hamartomatous disorders, but cerebriform hyperplasia of the soles and the palms is known as a quite distinctive characteristic in the dermatologic literature. the purpose of this case report is to demonstrate the mri features of plantar cerebriform hyperplasia in a 9-year-old boy with known proteus syndrome.200010741500
three-year surveillance study of nosocomial bacterial resistance in argentina. the antimicrobial committee; and the national surveillance program (sir) participants group.a national surveillance program (sir) was introduced in 1996 in argentina by the antimicrobial committee of the argentinean society for microbiology to assess bacterial resistance. the present study reports the rates of nosocomial bacterial resistance found by this program.200010737844
minimum inhibitory concentration determinations for various antimicrobial agents against 1570 bacterial isolates from turkey poults.minimum inhibitory concentrations (mics) were determined for 1570 bacteria from eight geographic locations (1204 escherichia coli, 231 other enteric gram-negative bacilli [including citrobacter spp., enterobacter spp., klebsiella spp., proteus spp., and salmonella spp.], 31 pseudomonas spp., 18 coagulase-positive staphylococci, 26 coagulase-negative staphylococci, and 55 streptococci and enterococci) by the national committee for clinical laboratory standards broth microdilution procedure. antim ...200010737648
treatment of gram-negative bacterial meningitis in term neonates with third generation cephalosporins plus amikacin.the aim of this retrospective study was to evaluate the clinical efficacy in terms of mortality and long-term morbidity of third generation cephalosporins and amikacin in combination for the treatment of gram-negative bacterial meningitis in a homogeneous group of neonates. a 15-year experience (1983-1997) with 72 term neonates without central nervous system anomalies and with gram-negative organisms grown in their cerebrospinal fluid treated with the above combination of antibiotics is presente ...200010729716
microbial populations and volatile compounds in the 'bone taint' spoilage of dry cured ham.'bone taint' is one of the most important causes of spoilage of dry cured ham. this alteration is characterized by a foul-smelling odour. the microbial population and volatile compounds associated with incipient 'bone taint' were evaluated. enterobacteriaceae species were found at levels of 7.94 x 10(5) cfu g(-1) in spoiled hams and were not detected in unspoiled hams. serratia sp. and proteus sp. are the main organisms characterized. the volatile compounds from the spoiled hams give rise to hig ...200010728563
comparable endotoxic properties of lipopolysaccharides are manifest in diverse clinical isolates of gram-negative bacteria.in general there is a poor correlation between serum lipopolysaccharide (lps; the biologically active constituent of endotoxin) levels and mortality in septic patients. the objective of this study was to determine if chemical, structural, or biological differences among lps from different clinical isolates of gram-negative bacteria might explain this discrepancy. lps preparations were made using the hot phenol-water extraction method from eight clinical isolates of gram-negative bacteria. as a p ...200010722580
nucleotide sequence of the bla(rtg-2) (carb-5) gene and phylogeny of a new group of carbenicillinases.we determined the nucleotide sequence of the bla gene for the acinetobacter calcoaceticus beta-lactamase previously described as carb-5. alignment of the deduced amino acid sequence with those of known beta-lactamases revealed that carb-5 possesses an rtg triad in box vii, as described for the proteus mirabilis gn79 enzyme, instead of the rsg consensus characteristic of the other carbenicillinases. phylogenetic studies showed that these rtg enzymes constitute a new, separate group, possibly ance ...200010722515
ciprofloxacin in the treatment of chronic bacterial prostatitis: a prospective, non-comparative multicentre clinical trial with long-term follow-up. the german prostatitis study group.the efficacy and safety of ciprofloxacin 500 mg bd for 28 days were assessed in 65 adult males with symptomatic bacterial prostatitis, from eight centres in germany. urine and prostatic secretions were obtained for culture. clinical signs and symptoms were evaluated at 12-18 days during treatment, end of treatment (4-9 days post-treatment), and 1, 3, 6 and 9 months post-treatment. safety was monitored during and at the end of treatment. e. coli was the most frequent pathogen causing infection (3 ...200010720805
ctx-m-type beta-lactamases: an emerging group of extended-spectrum enzymes.ctx-m-type beta-lactamases constitute a novel group of class a beta-lactamases with extended-spectrum properties. they are encoded by transferable plasmids and found in various enterobacteria, mostly salmonella typhimurium, escherichia coli, klebsiella pneumoniae and proteus mirabilis. ctx-m enzymes share extensive sequence similarity with the chromosomal beta-lactamases of klebsiella oxytoca. they efficiently hydrolyze many newer broad-spectrum oximino-beta-lactams including cefotaxime, ceftria ...200010720804
production of african breadfruit (treculia africana) and soybean (glycine max) seed based food formulations, 2: effects of germination and fermentation on microbiological and physical properties.the effects of germination (g) and naturally fermented (f) on the bacterial flora, viscosities and moisture sorption isotherms of soybean (s) and african breadfruit (b) seed based food products were investigated. bacillus, enterobacter, enterobacteriaceae, proteus, serratia and staphylococcus species dominated in the nonfermented products. lactobacillus, leuconostoc, pediococcus and yeast species dominated in the fermented products whose gruels also inhibited growth of coagulase positive staphyl ...199910716402
structure of the o-specific polysaccharide of the bacterium proteus mirabilis o29.an o-specific polysaccharide was obtained by mild acid degradation of p. mirabilis o29 lipopolysaccharide (lps) and found to contain 2-acetamido-2-deoxy-d-galactose and d-glucuronic acid (d-glca) in the ratio 3:1. studies of the polysaccharide by 1h- and 13c-nmr spectroscopy including two-dimensional correlation spectroscopy (cosy), total correlation spectroscopy (tocsy), nuclear overhauser effect spectroscopy (noesy), and h-detected 1h,13c-heteronuclear multiple-quantum coherence (hmqc) experim ...200010713543
[the resistance of recent clinical isolates against isepamycin, other aminoglycosides and injectable beta-lactams].clinical isolates collected from clinical facilities across japan in 1998 were tested against five aminoglycosides and three beta-lactams. the resistance of 50 strains each of methicillin sensitive staphylococcus aureus, methicillin resistant staphylococcus aureus (mrsa), staphylococcus epidermidis, escherichia coli, citrobacter freundii, klebsiella pneumoniae, enterobacter sp., serratia sp., pseudomonas aeruginosa and proteus sp. (p. mirabilis 25 strains and p. vulgaris 25 strains) to the amino ...200010709145
synthesis and antibacterial activity of 1-(3-hydroxy-2-naphthoyl)-4-substituted thiosemicarbazides.1-(3-hydroxy-2-naphthoyl)-4-substituted thiosemicarbazides were obtained by the addition of 3-hydroxy-2-naphthoic acid hydrazide to various isothiocyanates. the structures of the synthesized compounds were confirmed using uv and 1h-nmr spectral methods together with elemental analysis.199910707124
trends of antimicrobial resistance in bacterial isolates from a small animal referral hospital.a longitudinal, retrospective investigation was made of antimicrobial resistance in bacterial isolates obtained from clinical cases in a small animal hospital between 1989 and 1997. isolates of escherichia coli and staphylococcus species were used as gram-negative and gram-positive indicator organisms, respectively, and the annual prevalence of antimicrobial resistance was calculated for each organism to each of nine (for e coli) and 11 (for staphylococcus species) appropriate antimicrobials, in ...200010706308
[typing of extended-spectrum beta-lactamase-producing salmonella typhimurium strains isolated in a pediatric unit].extended-spectrum b-lactamases (esbls) derive mainly from tem and shv b-lactamases. these enzymes confer resistance to all oxyimino cephalosporins and monobactams except cephamycins and carbapems. esbls are often encoded by large plasmids that carry resistance determinants to multiple antibiotics and spread among the members of the enterobacteriaceae. since the first outbreak of klebsiella pneumoniae expressing an extended-spectrum beta-lactamase reported in 1984, nosocomial infections due to en ...199910705312
trends in the susceptibilities of proteus mirabilis isolates to quinolones. 200010702570
evaluation of the discriminatory powers of the dienes test and ribotyping as typing methods for proteus mirabilis.a total of 63 clinical isolates of proteus mirabilis collected over a 19-month period were typed by the dienes test and ribotyping. ribotyping was performed using the fully automated riboprinter microbial characterization system (qualicon, wilmington, del.). isolates that were indistinguishable by the dienes test and/or ribotyping were characterized further by pulsed-field gel electrophoresis (pfge). most of the isolates represented unique strains as judged by the dienes test and ribotyping. for ...200010699000
mixed infection in adult bacterial meningitis.12 adult patients suffering from bacterial meningitis caused by mixed infection were identified at kaohsiung chang gung memorial hospital over a period of 13 years (1986-1998), and they accounted for 6.5% (12/184) of our culture-proven adult bacterial meningitis. the 12 cases included seven males and five females, aged 17-74 years. six of the 12 cases had community-acquired infections and the other six had nosocomially-acquired infections. ten of the 12 cases had associated underlying diseases, ...200010697784
occurrence and antibiotic sensitivity of enterobacteriaceae isolated from a group of jordanian patients with community acquired urinary tract infections.the type and antibiotic sensitivity of urinary tract pathogens may differ in various communities. of 207 isolates recovered from midstream urine specimens collected from a group of patients with community acquired urinary tract infections (uti), 86% were species of enterobacteriaceae. the most frequently recovered pathogens were escherichia coli (82%), klebsiella spp. (7.3%), proteus spp. (6.2%), enterobacter spp. (3.4%) and citrobacter spp. (1.1%). high rates of resistance were found against am ...200010697742
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