Publications

influence of human wet cerumen on the growth of common and pathogenic bacteria of the ear.	the available data on the effect of human wet cerumen on bacterial growth are not conclusive. nevertheless it is widely accepted that cerumen has a bactericidal effect. in this study the activity of human wet cerumen on bacterial growth was assessed by applying cerumen suspensions to bacterial cultures. bacterial counts were performed before and after application of cerumen suspensions. a total of 383 assays was carried out with 73 pools of cerumen that were tested against cultures of staphyloco ...	2000	11177359
hypertrophic toe in a 44-year-old man.		2001	11176696
in vitro and in vivo antimicrobial activity of topical ofloxacin and other ototopical agents.	ototopical agents are extensively used for otitis externa (oe), acute otitis media identified by otorrhea in patients who have tympanostomy tubes (aom-tt) and chronic suppurative otitis media (csom). the quinolones have particular value as ototopical agents because of the broad spectrum of antibacterial activity of importance in otic diseases and the high concentrations of antibacterial activity at the site of infection.	2001	11176586
resistance patterns among nosocomial pathogens: trends over the past few years.	multiple surveillance studies have demonstrated that resistance among prevalent pathogens is increasing at an alarming rate, leading to greater patient morbidity and mortality from nosocomial infections. among gram-positive organisms, the most important resistant pathogens are methicillin- (oxacillin-)resistant staphylococcus aureus, beta-lactam-resistant and multidrug-resistant pneumococci, and vancomycin-resistant enterococci. important causes of gram-negative resistance include extended-spect ...	2001	11171776
urinary tract pathogens in complicated infection and in elderly individuals.		2001	11171003
acute mastoiditis--the antibiotic era: a multicenter study.	to evaluate the clinical course and identify the causative organisms of acute mastoiditis in a community where most of the patients who develop acute otitis media are treated with antibiotics.	2001	11165635
properties of multidrug-resistant, esbl-producing proteus mirabilis isolates and possible role of beta-lactam/beta-lactamase inhibitor combinations.	at our institution, isolation rates of clinical strains of esbl-producing proteus mirabilis increased to 8.8% of all p. mirabilis isolates during the period 1997-1999. to evaluate the susceptibility of esbl-producing p. mirabilis strains against commonly used drugs, we studied 50 non-duplicated isolates selected on the basis of synergy between clavulanate and beta-lactams (ceftazidime, aztreonam, cefotaxime, and ceftriaxone). the presence of esbl-coding genes was confirmed by colony hybridizatio ...	2001	11165117
proteus syndrome: clinical and surgical aspects.	the authors report a new case of proteus syndrome with delayed diagnosis and they discuss the place of surgery in this syndrome.	2000	11164195
interaction of proteus mirabilis urease apoenzyme and accessory proteins identified with yeast two-hybrid technology.	proteus mirabilis, a gram-negative bacterium associated with complicated urinary tract infections, produces a metalloenzyme urease which hydrolyzes urea to ammonia and carbon dioxide. the apourease is comprised of three structural subunits, urea, ureb, and urec, assembled as a homotrimer of individual ureabc heterotrimers (ureabc)(3). to become catalytically active, apourease acquires divalent nickel ions through a poorly understood process involving four accessory proteins, ured, uree, uref, an ...	2001	11157956
ascorbate conversion to oxalate in alkaline milieu and proteus mirabilis culture.	ascorbate breakdown reportedly accounts for 30% to 55% of urinary oxalate excreted. three potential degradation routes can be postulated: bowel, endogenous, and urinary. because the ph of normal urine ranges from 4.5 to 8.0, the urinary oxalate concentration in the presence of ascorbate may be influenced by urinary ph and environment, so we studied ascorbate conversion to oxalate in standard buffer solution and in urine. about 10% of infection stones associated with proteus mirabilis are reporte ...	2000	11156698
plasmid-mediated cephalosporinase acc-1 in clinical isolates of proteus mirabilis and escherichia coli.		2000	11152321
suction pressure can induce uncoupling of the plasma membrane from cortical actin.	we tested the hypothesis that a pressure difference can cause blebbing associated with uncoupling of the plasma membrane from the cortical actin, a phenomenon found earlier in locomoting blebbing walker carcinosarcoma cells. untreated, initially spherical walker carcinosarcoma cells were exposed to suction pressure by partial aspiration into micropipettes. the suction pressure required to induce blebbing was in the range of 0.9-3 cm h2o, i.e., somewhat lower than the increase in intracellular pr ...	2000	11152288
[selective intestinal bacterial decontamination in experimental acute pancreatitis].	bacterial translocation implies the transit of viable gastric microflora from the intestinal lumen through the intestinal wall to the mesenteric ganglia and seems to be the mechanism by which microflora reach necrotic tissue in acute pancreatitis (ap). this occurs in 40-70% of necrotizing hemorrhagic ap and plays a major role in up to 80% of deaths from this cause.	2000	11149219
[bact-alert system for hemocultures: evaluation of terminal subcultures].	few laboratory microbiological procedures are as important as the isolation of microorganisms from blood. to evaluate the usefulness of the terminal subcultures, 5669 blood cultures giving negative results after 7 days of incubation in the bact/alert system (organon teknika) were studied. bottles were distributed as follows: 1562 adult aerobic bottles, 119 adult anaerobic bottles, 3960 pediatric bottles and 28 fan bottles. from 5669 blood cultures, 10 subcultures that yielded growth had not been ...	2000	11149151
catheter-associated urinary tract infections.	in the past few years it has been clearly demonstrated that the concept of bacterial biofilm production permits an understanding and provides some explanation of the pathogenesis, diagnosis and treatment of catheter-associated urinary tract infections. this concept describes the colonization of catheter surfaces and the movement of bacteria against the urinary flow. it explains the antibacterial resistance of these matrix-enclosed sessile populations of bacteria. the catheter encrustation can be ...	2001	11148750
flagellin, a novel mediator of salmonella-induced epithelial activation and systemic inflammation: i kappa b alpha degradation, induction of nitric oxide synthase, induction of proinflammatory mediators, and cardiovascular dysfunction.	gram-negative sepsis is mediated by the actions of proinflammatory genes induced in response to microbes and their products. we report that flagellin, the monomeric subunit of flagella, is a potent proinflammatory species released by salmonella. flagellin (1 microgram/ml) induces ikappabalpha degradation, nf-kappab nuclear translocation, and inducible no synthase expression in cultured intestinal epithelial cells (iec). aflagellic salmonella mutants do not induce nf-kappab activation or no produ ...	2001	11145708
sudden death caused by pulmonary thromboembolism in proteus syndrome.	we report 3 patients with proteus syndrome (ps) who died suddenly from pulmonary embolism (pe). the first patient was a male diagnosed with ps at 12 years who had varicose veins, portal vein thrombosis, right iliac vein occlusion and recurrent pe. at age 25 years, he was admitted to the hospital with a severe headache. despite therapeutic doses of warfarin, investigations for an acute episode of breathlessness showed pe and he was unable to be resuscitated. the second case was a 9-year-old male ...	2000	11140839
conserved regulatory elements of the promoter sequence of the gene rpoh of enteric bacteria.	the rpoh regulatory region of different members of the enteric bacteria family was sequenced or downloaded from genbank and compared. in addition, the transcriptional start sites of rpoh of yersinia frederiksenii and proteus mirabilis, two distant members of this family, were determined. sequences similar to the sigma(70) promoters p1, p4 and p5, to the sigma(e) promoter p3 and to boxes dnaa1, dnaa2, camp receptor protein (crp) boxes crp1, crp2 and box cytr present in escherichia coli k12, were ...	2001	11139607
behavioural, physiological and metabolic responses to long-term starvation and refeeding in a blind cave-dwelling (proteus anguinus) and a surface-dwelling (euproctus asper) salamander.	the effects of long-term starvation and subsequent refeeding on haematological variables, behaviour, rates of oxygen consumption and intermediary and energy metabolism were studied in morphologically similar surface- and cave-dwelling salamanders. to provide a hypothetical general model representing the responses of amphibians to food stress, a sequential energy strategy has been proposed, suggesting that four successive phases (termed stress, transition, adaptation and recovery) can be distingu ...	2001	11136613
assessment of the capacity of lactobacillus to inhibit the growth of uropathogens and block their adhesion to vaginal epithelial cells.	to gain insight into the mechanisms by which lactobacillus blocks the adherence of uropathogens to vaginal epithelial cells and inhibits their growth, 15 lactobacillus strains and 22 uropathogens were studied. lactobacilli from hemagglutination group iii, identified as lactobacillus crispatus, showed greater capacity to block uropathogen adherence than those from hemagglutination groups ii and i (61.9%, 49.5%, and 52.6% of blockage, respectively). pseudomonas aeruginosa pa5 and klebsiella pneumo ...	2001	11133381
clostridium difficile toxins a and b can alter epithelial permeability and promote bacterial paracellular migration through ht-29 enterocytes.	clostridium difficile toxins a and b are the widely recognized etiologic agents of antibiotic-associated diseases ranging from diarrhea to pseudomembranous colitis. we hypothesized that c. difficile toxins may alter intestinal epithelial permeability and facilitate bacterial penetration of the intestinal epithelial barrier. experiments were designed to clarify the effects of c. difficile toxins a and b on the flux of inert particles across ht-29 enterocyte monolayers, and to correlate these resu ...	2000	11131913
human anti-scrub typhus rickettsia and rabbit anti-proteus antibodies recognize similar epitope in the o-polysaccharide part of proteus mirabilis oxk lipopolysaccharide.	in the weil-felix test, sera from patients infected with orientia tsutsugamushi reacted with lipopolysaccharide (lps) from proteus mirabilis oxk strains. the o-polysaccharide of p. mirabilis oxk lps consisted of pentasaccharide repeating units, with amidically-linked lysine residues. the lysine, linked to galacturonic residues, which plays an important role in the reaction with rabbit anti-oxk antibodies, was revealed with the aid of synthetic antigens. using elisa, immunoglobulin m antibodies f ...	2000	11131808
evaluation of a method using proteus mirabilis and pseudomonas aeruginosa to experimentally induce dual infection of the urinary bladder in dogs.	to evaluate a method using proteus mirabilis and pseudomonas aeruginosa to experimentally induce dual infection of the urinary bladder in dogs.	2000	11131585
interaction of actin filaments with the plasma membrane in amoeba proteus: studies using a cell model and isolated plasma membrane.	we prepared a cell model of amoeba proteus by mechanical bursting to study the interaction between actin filaments (afs) and plasma membrane (pm). the cell model prepared in the absence of ca2+ showed remarkable contraction upon addition of atp. when the model was prepared in the presence of ca2+, the cytoplasmic granules formed an aggregate in the central region, having moved away from pm. although this model showed contraction upon addition of atp in the presence of ca2+, less contraction was ...	2000	11129797
influence of intestinal flora on the development of fibrosis and cirrhosis in a rat model.	the gut flora play a significant role in the disposition of many foreign substances, as well as producing nutrients and toxins that may be absorbed and reach the liver. this study examines the influence of antibiotic-induced alterations in gut flora on the development of hepatic fibrosis in a rat model.	2000	11129226
in vitro antimicrobial activity of propolis dry extract.	in this study the antibacterial and antifungal properties of propolis, a natural product of bees, have been investigated against different pathogens. minimum inhibitory concentrations (mics) and minimum bactericidal concentrations (mbcs) were determined according to nccls standards on 320 strains including staphylococcus aureus, group a beta-hemolytic streptococci, streptococcus pneumoniae, moraxella catarrhalis, haemophilus influenzae, klebsiella pneumoniae, escherichia coli, proteus mirabilis, ...	2000	11128558
bacterial adherence to silver nitrate coated poly-l-lactic acid urological stents in vitro.	the purpose of this study was to see whether it is possible to prevent bacterial adherence to bioabsorbable self-reinforced l-lactic acid polymer (sr-plla) urological stents. the sr-plla stents were coated with silver nitrate blended epsilon-caprolactone/l-lactide copolymer. the adherence of five bacterial strains (pseudomonas aeruginosa, enterococcus faecalis, proteus mirabilis and two strains of escherichia coli) to coated and non-coated sr-plla wires were tested. it was found that silver nitr ...	2000	11127712
changing pattern of antimicrobial susceptibility of organisms causing community acquired urinary tract infections.	to assess common organisms causing urinary treat infection (uti) in this community and to see antimicrobial susceptibility pattern of these isolates.	2000	11126812
genetic diversity in proteus mirabilis isolates found in the urinary tract of rheumatoid arthritis patients.	elevated levels of anti-proteus antibodies but not antibodies to e. coli have been reported in patients with rheumatoid arthritis (ra). the suggestion has been made that p. mirabilis may have a role in the aetiopathogenesis of rheumatoid arthritis. the aim of this study was to determine whether there are differences at the genetic level inisolates of p. mirabilis obtained from controls and ra patients.	2000	11120612
bacterial adherence to ofloxacin-blended polylactone-coated self-reinforced l-lactic acid polymer urological stents.	to determine whether ofloxacin coating has any effect on bacterial adherence to bioresorbable self-reinforced l-lactic acid polymer (sr-plla) urological stents. materials ans methods: sr-plla stents were coated with epsilon-caprolactone/l-lactide copolymer blended with ofloxacin at three different concentrations of ofloxacin (0.5, 2 and 5% w/w). the adherence of five bacterial strains (pseudomonas aeruginosa, enterococcus faecalis, proteus mirabilis and two strains of escherichia coli) to the co ...	2000	11119087
beta-lactam susceptibility patterns and investigation of cephalosporin hydrolysing beta-lactamases of gram-negative extraintestinal clinical isolates.	of more than 3500 isolates of enterobacteriaceae, 48-69% were resistant to aminopenicillins and 11-45% to amoxycillin+clavulanic acid. resistance to second and third generation cephalosporins was present in 11-17 and 3-8% of escherichia coli, 47-56 and 15-52% of klebsiella-enterobacter, 36-57 and 16-27% of proteus, providencia and morganella isolates. pseudomonas aeruginosa strains varied in their resistance to antipseudomonal beta-lactams. isoelectric points, inhibitor profiles and substrate pr ...	2000	11118847
full structure of the o-specific polysaccharide of proteus mirabilis o24 containing 3,4-o-[(s)-1-carboxyethylidene]-d-galactose.	the structure of a neutral polysaccharide isolated by degradation with dilute acetic acid of the lipopolysaccharide (lps) of p. mirabilis o24 has been determined recently [e. literacka et al., febs lett., 456 (1999) 227-231]. further studies of this lps using alkaline degradation and hydrolysis at ph 4.5 showed that the polysaccharide chain includes an acetal-linked pyruvic acid residue, which is removed completely during delipidation with acetic acid. a revision using 1h and 13c nmr spectroscop ...	2000	11117329
the structure of the carbohydrate backbone of the core-lipid a region of the lipopolysaccharide from proteus mirabilis serotype o28.	the following structure of the lipid a-core region of the lipopolysaccharide (lps) from proteus mirabilis serotype o28 was determined using nmr, ms, and chemical analysis of the core oligosaccharide, obtained by mild acid hydrolysis of lps, and of the products of alkaline deacylation of the lps: carbohydrate sequence [see text] where s = beta-galalys (amide of beta-d-galactopyranosyluronic acid with the alpha-amino group of l-lysine) or beta-galalys4petn are present in the ratio of approximately ...	2000	11117318
orbital abscess: management and outcome.	to discuss the diagnosis, management and outcome of various types of orbital abscess.	2000	11116509
otogenic brain abscess: review of 41 cases.	forty-one patients in whom otogenic brain abscess was diagnosed and has been treated since 1968 are presented. sixty-five percent of the patients were between 5 and 15 years of age. all patients had chronic otitis media, and 95% had cholesteatoma. abscess was located in the temporal lobe in 54%, in the cerebellum in 44%, and in both locations in 2% of the cases. most patients had radical mastoidectomy and evacuation of the abscess through the mastoidectomy (61%). in addition to mastoidectomy, bu ...	2000	11112974
impact of fluoroquinolone administration on the emergence of fluoroquinolone-resistant gram-negative bacilli from gastrointestinal flora.	we assessed the risk factors for acquisition of fluoroquinolone-resistant, gram-negative organisms in the gastrointestinal tract of hospitalized patients. we analyzed stool samples from 204 patients and recovered fluoroquinolone-resistant, gram-negative organisms from 63. receipt of fluoroquinolone during the month preceding admission was the only risk factor identified, whereas female sex, duration of hospitalization, exposure to indwelling devices, admission from another hospital, and history ...	2001	11112677
new serogroups of the genus proteus consisting of proteus penneri strains only. determination of some lps epitopes responsible for specificity.		2000	11109127
use of randomly amplified polymorphic dna (rapd) analysis for identification of proteus penneri.		2000	11109123
determination of genetic diversity of proteus penneri strains using rep-pcr.		2000	11109122
epitope specificity of polyclonal rabbit antisera against proteus vulgaris o-antigens.		2000	11109112
effect of spontaneous and induced mutations on outer membrane proteins and lipopolysaccharides of proteus penneri strain 357.		2000	11109104
[infectivity and resistance to antibiotics of uropathogenic gram-negative rods].	the nature of bacterial isolates from children with clinically suspected urinary tract infections (uti) was studied. the susceptibility of urinary pathogens to selected antibiotics was determined. the results clearly show that e. coli was identified as the main causative agent of uti children (67% of isolates). the second commonest pathogen was p. mirabilis (10%). over half e. coli isolates were resistant to amino-penicilins but almost all isolates (over 80%) were sensitive to antimicrobial agen ...	2000	11107786
drug resistance in bacteria isolated from a brazilian hospital.	bacteria commonly associated with cases of hospital infection were isolated from samples of food, from food handless, and from objects and surfaces from different places of a hospital in piracicaba, são paulo, brazil, and the resistance patterns to antibiotic of these strains of bacteria were evaluated. the resistance patterns of these bacteria showed a large variation, and a high frequency of resistance to ampicillin (60.9%), cephalothin (58.7%) and carbenicillin (52.2%) was observed. the frequ ...	1998	11103021
cost-effective and rapid presumptive identification of gram-negative bacilli in routine urine, pus, and stool cultures: evaluation of the use of chromagar orientation medium in conjunction with simple biochemical tests.	the algorithm for a new identification system was designed on the basis of colony color and morphology on chromagar orientation medium in conjunction with simple biochemical tests such as indole (ind), lysine decarboxylase (ldc), and ornithine decarboxylase (odc) utilization tests with gram-negative bacilli isolated from urine samples as well as pus, stool, and other clinical specimens by the following colony characteristics, biochemical reactions, and serological results: pinkish to red, ind po ...	2000	11101600
pathogenesis of proteus mirabilis urinary tract infection.	proteus mirabilis is a causative agent of cystitis and pyelonephritis primarily in individuals with indwelling catheters or structural abnormalities of the urinary tract. the organism produces a variety of unique virulence factors that contribute to its pathogenicity and persistence in the human host.	2000	11099936
molecular mimicry between hla-dr alleles associated with rheumatoid arthritis and proteus mirabilis as the aetiological basis for autoimmunity.	molecular mimicry is one of the pathological mechanisms proposed to explain the association between microorganisms and autoimmune diseases. this review deals with the association between bacteria and rheumatic diseases with a special emphasis on rheumatoid arthritis where upper urinary tract infection by proteus mirabilis is the possible cause of this severe, arthritic condition. prospective trials involving anti-proteus therapy should be carried out.	2000	11099935
selective medium for primary isolation of members of the tribe proteeae.	a selective proteeae medium (spm) for isolation and preliminary detection of species of genera proteus, morganella, and providencia was evaluated. the spm contains tryptose phosphate agar with phenolphthalein monophosphate (as substrate for phosphatase activity), bile salts and polymyxin b (as inhibitors). the selectivity of the spm was tested by the ecometric method of quality assurance of culture media. fourteen reference cultures of enterobacteria and fifty-four strains of proteeae were teste ...	1999	11097023
the structure of the carbohydrate backbone of the core-lipid a region of the lipopolysaccharide from proteus vulgaris serotype o25.	the following structure of the lipid a-core region of the lipopolysaccharide (lps) from proteus vulgaris serotype o25 was determined by using nmr and chemical analysis of the core oligosaccharide, obtained by mild acid hydrolysis of lps, of the products of alkaline deacylation of the lps, and of the products of lps deamination: [structure: see text] terminal residues of beta-glcnac and beta-kdo (indicated by bold italics) are present alternatively in approximately 3:2 amount, leaving no unsubsti ...	2000	11093709
in vitro activity of gatifloxacin compared with gemifloxacin, moxifloxacin, trovafloxacin, ciprofloxacin and ofloxacin against uropathogens cultured from patients with complicated urinary tract infections.	minimum inhibitory concentrations (mics) of gatifloxacin were compared with those of gemifloxacin, moxifloxacin, trovafloxacin, ciprofloxacin and ofloxacin using an agar dilution method for 400 uropathogens cultured from the urine of urological patients with complicated and/or hospital-acquired urinary tract infections (uti). the collection of strains was made up of enterobacteriaceae (34.5%), enterococci (31.5%), staphylococci (21.2%) and non-fermenting bacteria (12.8%). the antibacterial activ ...	2000	11091042
structural and serological studies of the related o-specific polysaccharides of proteus vulgaris o21 and proteus mirabilis o48 having oligosaccharide-phosphate repeating units.	the o-specific polysaccharide chains (o-antigens) of the lipopolysaccharides (lpss) of proteus mirabilis o48 and proteus vulgaris o21 were found to have tetrasaccharide and pentasaccharide repeating units, respectively, interlinked by a glycosidic phosphate. polysaccharides and an oligosaccharide were derived from the lpss by various degradation procedures and studied by 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, noesy, h-detected 1h,13c and 1h,31p hmqc experiments. the following rel ...	2000	11082201
successful in situ treatment of an infected ascending aortic graft.	infection of an ascending aortic prosthesis is a grave complication associated with a high mortality. in most cases, extraanatomic bypass and removal of the infected vascular graft are not possible. furthermore, the standard approach to this problem, which includes excision and replacement or debridement and repair of infected thoracic aortic grafts, carries a high early mortality. we report the successful treatment of this life-threatening complication using a conservative strategy in which the ...	2000	11081915
periplasmic localization of a groes homologue in escherichia coli transformed with groesx cloned from legionella-like endosymbionts in amoeba proteus.	escherichia coli mc4100 transformed with a groe homologous operon cloned from x-bacteria accumulated large amounts of the gene product when cultured at 30 or 37 degrees c. heat shock for 10-30 min at 42 degrees c or ethanol (5%) shock for 2 h increased groesx levels to about twice that in e. coli grown at 30 degrees c. the subcellular localization of groesx in transformed e. coli was determined by several subcellular fractionation methods, by the analysis of extracted proteins in sds polyacrylam ...	2000	11081576
[bacterial flora of the urinary tract and its drug sensitivity in children with vesico-ureteral reflux].	the authors of the following publication present the results of a research which has been made on the presence of bacterial flora in children with vesico-ureteral reflux. as a first step, an analysis of the bacterial flora was made. then, two pathogens occurring most often were selected their drug-sensitivity was tested. the assessment of the bacterial flora and its drug-sensitivity ware based on urine inoculations. clinical material was provided by 184 children suffering from urinary tract infe ...	2000	11081344
isolation using triflic acid solvolysis and identification of n(epsilon)-[(r)-1-carboxyethyl]-n(alpha)-(d-galacturonoyl)-l-lysine as a component of the o-specific polysaccharide of proteus mirabilis o13.	an amino acid was released from the o-specific polysaccharide of proteus mirabilis o13 by acid hydrolysis and identified as n(epsilon)-[(r)-1-carboxyethyl]-l-lysine by comparison with the authentic sample. an amide of this amino acid with d-galacturonic acid was isolated from the polysaccharide by solvolysis with anhydrous trifluoromethanesulfonic (triflic) acid and characterised by 1h and 13c nmr spectroscopy. these and published data enabled determination of the full structure of the repeating ...	2000	11072853
the binding of proteus mirabilis nonagglutinating fimbriae to ganglio-series asialoglycolipids and lactosyl ceramide.	proteus mirabilis is a common opportunistic gram-negative uropathogen that infects the upper urinary tract. we have examined the role of the nonagglutinating fimbriae (naf) of p. mirabilis in mediating bacterial adhesion to cell surface receptors. purified naf of p. mirabilis were demonstrated to bind to a number of glycolipids, including asialo-gm1, asialo-gm2, and lactosyl ceramide (laccer) in solid-phase binding assays and in thin layer chromatography (tlc) overlay assays. furthermore, preinc ...	2000	11068685
integron-located veb-1 extended-spectrum beta-lactamase gene in a proteus mirabilis clinical isolate from vietnam.	a clinical isolate of proteus mirabilis lil-1 was obtained from a vietnamese patient hospitalized in paris, france. this isolate was resistant to cephalosporins, and there was marked synergy between cephalosporins and clavulanic acid together with unusual synergy between cefoxitin and cefuroxime. pcr analysis revealed the presence of blaveb-1, an integron-located gene coding for an extended-spectrum beta-lactamase (esbl) identified previously in an escherichia coli isolate mg-1 from vietnam. usi ...	2000	11062188
[proteus syndrome].		2000	11057145
proteus virulence: involvement of the pore forming alpha-hemolysin (a short review).	the genus proteus belongs to the tribe of proteae in the family of enterobacteriaceae, and consists of five species: p. mirabilis, p. vulgaris, p. morganii, p. penneri and p. myxofaciens. they are distinguished from the rest of enterobacteriaceae by their ability to deaminate phenylalanine and tryptophane. they hydrolyze urea and gelatin and fail to ferment lactose, mannose, dulcitol and malonate; and do not form lysine and arginine decarboxylase or beta-galactosidase [1]. colonies produce disti ...	2000	11056765
cerebriform plantar hyperplasia: ultrastructural study of two cases.	in the present work we report the histopathological features of the cerebriform plantar hyperplasia observed in two patients with a mild form of the proteus syndrome. light microscopy revealed increased fibro-adipose tissue and adnexal structures in the dermis. ultrastructurally, densely packed collagen fibrils variable in diameter and configuration, described as composite fibrils and unraveled fibrils, as well as a few fragmented elastic fibrils presenting an altered ratio between the elastin a ...	2000	11056430
functions of the subunits in the flhd(2)c(2) transcriptional master regulator of bacterial flagellum biogenesis and swarming.	in enterobacteria like salmonella, biogenesis of cell surface flagella needed for motility is dependent upon the master operon flhdc at the apex of the flagellar gene hierarchy. the operon products flhd and flhc act together in a flhd(2)c(2 )heterotetramer to induce flagellar gene transcription, while flhd also represses cell septation. the flhdc operon is pivotal to differentiation into elongated hyperflagellated swarm cells that undergo multicellular migration, most strikingly in proteus. we s ...	2000	11054284
complement activation by proteus mirabilis negatively charged lipopolysaccharides.	proteus mirabilis strains are human pathogens responsible for urinary tract infections and bacteremias and may be involved in rheumatoid arthritis. lipopolysaccharide (lps, bacterial endotoxin), the major component of the cell wall, is one of the virulence factors of proteus. in the presented studies, we have investigated complement activation by lpss isolated from p. mirabilis o10, o23, o30, and o43 strains, which differ in the number of negative coo- groups on their polysaccharide components. ...	2000	11052177
anaesthesia for proteus syndrome.	a 4-month-old boy with proteus syndrome underwent a successful operation for a left abdominal mass due to hydroureter and hydronephrosis with left ureterovesical stenosis. the operation lasted 4.5 h under general anaesthesia; there were no anaesthetic complications. there is only one previous report on anaesthesia in a patient with proteus syndrome.	2000	11050524
proteus syndrome.		2000	11044199
structure of the o-specific polysaccharide of the bacterium proteus vulgaris o23.	an acidic o-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the bacterium proteus vulgaris o23 (strain prk 44/57) and found to contain 2-acetamido-2-deoxy-d-galactose, 2-acetamido-2-deoxy-d-glucose, and d-galacturonic acid. based on 1h- and 13c-nmr spectroscopic studies, including two-dimensional correlation spectroscopy (cosy), total correlation spectroscopy (tocsy), nuclear overhauser effect spectroscopy (noesy), and 1h,13c heteronuclear multiple-quan ...	2000	11042498
[transfer of resistance to 3rd generation cephalosporins and aztreonam in strains of klebsiella pneumoniae producing extended spectrum beta-lactamases].	the authors demonstrated the transferability of antibiotic resistance genes in nosocomial strains of klebsiella pneumoniae, isolated from newborn children at the paediatric university hospital in bratislava. strains were resistant to cefotaxime, ceftazidime and aztreonam. the determinants of resistance (carbenicillin, cephaloridine, cefotaxime, ceftazidime and aztreonam) were transferred to recipient strains of escherichia coli k-12 and proteus mirabilis p-38. the transfer of resistance determin ...	1999	11038673
a novel action of the proton pump inhibitor rabeprazole and its thioether derivative against the motility of helicobacter pylori.	the motility of helicobacter pylori was maximum at 37 degrees c and at ph 6. a newly developed proton pump inhibitor, rabeprazole (rpz), and its thioether derivative (rpz-th) markedly inhibited the motility of h. pylori. the concentrations of the drug necessary to inhibit 50% of the motility were 0.25, 16, 16, and >64 microgram/ml for rpz-th, rpz, lansoprazole, and omeprazole, respectively. no such inhibitory effects were observed with h(2) blockers or anti-h. pylori agents. the motilities of ca ...	2000	11036024
classification of proteus vulgaris biogroup 3 with recognition of proteus hauseri sp. nov., nom. rev. and unnamed proteus genomospecies 4, 5 and 6.	strains traditionally identified as proteus vulgaris formed three biogroups. biogroup 1, characterized by negative reactions for indole production, salicin fermentation and aesculin hydrolysis, is now known as proteus penneri. biogroup 2, characterized by positive reactions for indole, salicin and aesculin, was shown by dna hybridization (hydroxyapatite method) to be a genetic species separate from biogroup 1 and from biogroup 3 which is positive for indole production and negative for salicin an ...	2000	11034498
endotoxins stimulate generation of superoxide radicals and lipid peroxidation in blood platelets.	lipopolysaccharide (lps, endotoxin) is an important structural constituent of the membrane of gram-negative bacteria with a wide range of biological effects. it can activate blood platelets. the purpose of present study was to determine the direct effect of endotoxins from proteus mirabilis, differing significantly in their composition, on the generation of superoxide radicals and thiobarbituric acid reactive substances (tbars) in blood platelets. superoxide radicals were measured by means of su ...	2000	11034442
structure of an o-acetylated acidic o-specific polysaccharide of proteus vulgaris o46.	an acidic o-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of proteus vulgaris o46 and studied by chemical methods (o-deacetylation, sugar and methylation analyses, partial solvolysis) and 1h and 13c nmr spectroscopy. solvolysis of the o-deacetylated polysaccharide with trifluoromethanesulfonic acid resulted in a alpha-d-glcpnac-(1 --> 3)-d-glca disaccharide that demonstrated the usefulness of this reagent for selective cleavage of heteropolysaccharides. ...	2000	11028790
virulence of a proteus mirabilis atf isogenic mutant is not impaired in a mouse model of ascending urinary tract infection.	proteus mirabilis, a common cause of urinary tract infection, produces a number of different fimbriae, including ambient temperature fimbriae (atf). these fimbriae are optimally expressed at 23 degrees c and their contribution to urinary tract infection has so far remained unknown. in the present study, a clinical isolate of p. mirabilis and an isogenic allelic replacement mutant unable to express atf were tested for their ability to cause infection in the ascending urinary tract infection model ...	2000	11024353
classification, identification, and clinical significance of proteus, providencia, and morganella.	this review presents the current taxonomy of the genera proteus, providencia, and morganella, along with the current methods for the identification of each species within the three genera, incorporating both conventional biochemical and commercial methods. while all of these organisms are ubiquitous in the environment, individual case reports and nosocomial outbreak reports that demonstrate their ability to cause major infectious disease problems are presented. lastly, anticipated antimicrobial ...	2000	11023955
evaluation of the role of two conserved active-site residues in beta class glutathione s-transferases.	glutathione s-transferases (gsts) normally use hydroxy-group-containing residues in the n-terminal domain of the enzyme for stabilizing the activated form of the co-substrate, glutathione. however, previous mutagenesis studies have shown that this is not true for beta class gsts and thus the origin of the stabilization remains a mystery. the recently determined crystal structure of proteus mirabilis gst b1-1 (pmgst b1-1) suggested that the stabilizing role might be fulfilled in beta class gsts b ...	2000	11023819
dna sequence analysis of the tellurite-resistance determinant from clinical strain of escherichia coli and identification of essential genes.	the tellurite-resistant escherichia coli strain kl53 was found during testing of the group of clinical isolates for antibiotics and heavy metal ion resistance (burian et al. 1990). determinant of the tellurite resistance of the strain was located on the large conjugative plasmid pte53 and cloned into pacyc184. three different ter clones harboring plk2, plk18 and plk20 were isolated (burian et al. 1998). the smallest functional ter clone harboring plk18 was chosen for further analysis. plasmid pl ...	2000	11016400
reversible changes in size of cell nuclei isolated from amoeba proteus: role of the cytoskeleton.	micrurgically isolated interphasal nuclei of amoeba proteus, which preserve f-actin cytoskeletal shells on their surface, shrink after perfusion with imidazole buffer without atp, and expand to about 200% of their cross-sectional area upon addition of pyrophosphate. these changes in size may be reproduced several times with the same nucleus. the shrunken nuclei are insensitive to the osmotic effects of sugars and distilled water, whereas the expanded ones react only to the distilled water, showi ...	2000	11012088
evaluation of the intestinal microflora in the rat model for esophageal adenocarcinoma.	surgically induced duodenal reflux results in cancer development in the rat esophagus. one proposed mechanism of carcinogenesis relies on the production of carcinogens in the presence of bacterial overgrowth. against this background, intestinal microflora in the rat jejunum was analyzed before and after reflux-inducing surgery. total gastrectomy and esophagojejunostomy were performed on sprague-dawley rats to produce esophageal reflux of duodenal juice (n = 12). three days before surgery they we ...	2000	11005330
effects of nonpolar mutations in each of the seven bacillus subtilis mrp genes suggest complex interactions among the gene products in support of na(+) and alkali but not cholate resistance.	the bacillus subtilis mrp (multiple resistance and ph) operon supports na(+) and alkali resistance via an na(+)/h(+) antiport, as well as cholate efflux and resistance. among the individual mutants with nonpolar mutations in each of the seven mrp genes, only the mrpf mutant exhibited cholate sensitivity and a cholate efflux defect that were complemented by expression of the deleted gene in trans. expression of mrpf in the mrp null (vkn1) strain also restored cholate transport and increased na(+) ...	2000	11004162
capillary electrophoretic analysis of wild type and mutant proteus penneri outer membrane proteins.	in this study the virulence factors, outer membrane proteins (omp), lipopolysaccharides (lps), hemolysin, and the in vivo and in vitro virulence of wild-type proteus penneri 357 and its two isogenic mutant variants--a transposon and a spontaneous mutant--were examined. the omps of these variants were analyzed by a new and fast technique, "dynamic sieving" capillary electrophoresis (ce). the omp profiles were dominated by two peaks (39 and 43 kda). in the p. penneri clone examined, both the trans ...	2000	11001319
in-vitro antimicrobial activity of cefpirome: a new fourth-generation cephalosporin against clinically significant bacteria.	to study the in-vitro antimicrobial activity of cefpirome: a new fourth generation cephalosporin in comparison with other agents against clinically significant gram-negative and gram-positive bacteria.	2000	10992706
genetic characterization of dna region containing the trh and ure genes of vibrio parahaemolyticus.	we have demonstrated that possession of the gene for thermostable direct hemolysin-related hemolysin (trh) coincides with the presence of the urease gene among clinical vibrio parahaemolyticus strains and that the location of the two genes are in close proximity on the chromosome. here, we cloned and sequenced the 15,754-bp dna region containing the trh gene and the gene cluster for urease production from the chromosome of clinical v. parahaemolyticus (th3996). we found 16 open reading frames (o ...	2000	10992480
effect of subinhibitory concentrations of macrolides on expression of flagellin in pseudomonas aeruginosa and proteus mirabilis.	in the present study we showed by molecular analysis that the inhibition of motility by macrolides in proteus mirabilis and pseudomonas aeruginosa was well correlated with the loss of the expression of flagellin. erythromycin, clarithromycin, and azithromycin at subinhibitory concentrations (sub-mics) suppressed the expression of flagellin dose dependently. azithromycin had the strongest inhibitory effect on the expression of p. aeruginosa flagellin, whereas 16-membered rokitamycin had only a we ...	2000	10991876
lymphopenia in lymphatic malformations.		2000	10991755
macrodactyly: report of eight cases and review of the literature.	congenital enlargement of one or several digits of the hands or feet (macrodactyly) is a rare disorder. a considerable proportion of the patients with this condition are referred to dermatology departments. the majority of the cases reported in the literature represent hamartomas with combined hypertrophy of several, predominantly lipomatous, soft tissue components and overgrowth of bone. the differential diagnosis includes klippel-trenaunay-weber syndrome, neurofibromatosis, milroy disease, and ...	2000	10990574
reduction of endogenous bacteria associated with catfish fillets using the grovac process.	fresh catfish (ictalurus punctatus) fillets are known to be contaminated with a large number of spoilage and pathogenic bacteria. the grovac method, a new patented (u.s. 5,543,163) process, was evaluated for its efficacy in reducing the number of pathogens and spoilage microorganisms associated with food. this process involves using a processing solution containing ascorbic acid (aa) and sodium chloride (nacl), vacuum, and tumbling. a total of 51 bacterial isolates were isolated and identified f ...	2000	10983798
a "microtubule" in a bacterium.	a study of the anchorage of the flagella in swarmers of proteus mirabilis led to the incidental observation of microtubules. these microtubules were found in thin sections and in whole mount preparations of cells from which most of the content had been released by osmotic shock before staining negatively with potassium phosphotungstate (pta). the microtubules are in negatively stained preparations about 200 a wide, i.e. somewhat thicker than the flagella (approximately 130 a). they are thus some ...	1967	10976198
[psoas abscess: difficult initial clinical impression].		2000	10974773
extreme functional sensitivity to conservative amino acid changes on enzyme exteriors.	mutagenesis studies and alignments of homologous sequences have demonstrated that protein function typically is compatible with a variety of amino-acid residues at most exterior non-active-site positions. these observations have led to the current view that functional constraints on sequence are minimal at these positions. here, it is shown that this inference assumes that the set of acceptable residues at each position is independent of the overall sequence context. two approaches are used to t ...	2000	10966772
[immunosenescence: delayed cutaneous hypersensitivity tests in independently-living chilean elderly individuals].	delayed cutaneous hypersensitivity tests are a globally accepted test to assess cellular immunity in vivo. the quality and quantity of the response to these type of tests, varies in different populations.	2000	10962854
antimicrobial-resistance trends in bacterial isolates from companion-animal community practice in the uk.	we conducted a longitudinal, retrospective investigation of antimicrobial resistance in bacterial isolates obtained from canine and feline clinical cases in veterinary community practice in uk (1989-1997). individual-drug resistance was examined using isolates of escherichia coli and staphylococcus spp. as gram-negative and gram-positive indicator organisms, respectively. the annual prevalence of resistance was calculated for each organism to each of nine (for e. coli) and 11 (for staphylococcus ...	2000	10960713
[tartrate-sensitive and tartrate-resistant acid phosphatases in amoeba proteus].	in free-living amoeba proteus (strain b), acid phosphatase (acp) was examined by disc-electrophoresis in polyacrylamide gel. the tartrate-sensitive amebian acp was greatly inhibited by dithiothreitol and cu2+, and only partly inhibited by sodium orthovanadate, ammonium molybdate, edta, disodium salt and mg2+, ca2+, zn2+ and mn2+. on the contrary, it appeared to be resistant to sulfhydryl reagents--4(hydroxymercury) benzoic acid, sodium salt and n-ethylmaleimide. unlike the tartrate-sensitive enz ...	2000	10953868
[pyonephrosis: diagnosis and treatment: report of 14 cases].	prior to the introduction of antibiotics, the treatment of pyelonephrosis frequently consisted of nephrectomy to remove the non-functional kidney, which was a potentially dangerous source of systemic infection. this approach was later modified as a result of the advances made in antibiotic therapy, and included vigorous antibiotic treatment and prompt drainage of the kidney. at present, percutaneous nephrostomy provides a means of draining off the pus and determining a possible residual renal fu ...	2000	10953791
tem-72, a new extended-spectrum beta-lactamase detected in proteus mirabilis and morganella morganii in italy.	a new natural tem-2 derivative, named tem-72, was identified in a proteus mirabilis strain and in a morganella morganii strain isolated in italy in 1999. compared to tem-1, tem-72 contains the following amino acid substitutions: q39k, m182t, g238s, and e240k. kinetic analysis showed that tem-72 exhibits an extended-spectrum activity, including activity against oxyimino-cephalosporins and aztreonam. expression of bla(tem-72) in escherichia coli was capable of decreasing the host susceptibility to ...	2000	10952610
ibc-1, a novel integron-associated class a beta-lactamase with extended-spectrum properties produced by an enterobacter cloacae clinical strain.	a transferable beta-lactamase produced by a multidrug-resistant clinical isolate of enterobacter cloacae was studied. the bla gene was carried by a large (>80-kb) transmissible plasmid. nucleotide sequence analysis of cloned fragments revealed that it was part of a gene cassette carried by a class 1 integron along with other resistance genes, including aac(6')-ib. the encoded beta-lactamase, designated ibc-1, was a novel class a enzyme that hydrolyzed ceftazidime and cefotaxime and was inhibited ...	2000	10952563
cefdinir versus cefaclor in the treatment of uncomplicated urinary tract infection.	this multicenter, double-blind, randomized, parallel-group study was conducted in europe, south africa, and australia to compare the clinical and microbiologic efficacy and the tolerability of a cephalosporin antibiotic, cefdinir, with those of cefaclor in the treatment of uncomplicated urinary tract infection.	2000	10945508
bacteriology of chronic otitis media in dar es salaam, tanzania.	to determine the aetiology of chronic otitis media (com) in dar es salaam and to find out the shelf life of boric acid in spirit ear drops (baised).	2000	10944833
effect of initial carbon sources on the performance of microbial fuel cells containing proteus vulgaris.	mediator-coupled microbial fuel cells containing proteus vulgaris were constructed and the cell performance was tested. fuel cell efficiency depended on the carbon source in the initial medium of the microorganism. maltose and trehalose were not utilized substantially by p. vulgaris; however, their presence in the initial medium resulted in enhanced cell performance. in particular, galactose showed 63% coulombic efficiency in a biofuel cell after p. vulgaris was cultured in a trehalose-containin ...	2000	10940867
prevalence of multidrug resistant organisms in an intensive care burn unit.	during january to december, 1998, analysis of an outbreak of infections in the burn intensive care unit (bicu) of the hospital attached to the dayanand medical college, ludhiana was carried out. a total of 868 clinical samples from 290 patients with more than 40 per cent thermal injury were investigated. these samples included 322 wound swabs, 325 blood and 221 urine samples. bacterial pathogens were isolated from 80, 62 and 48 per cent samples of pus, blood and urine respectively. among the nin ...	2000	10935317
[renocolic fistula: a case report].	a 78-year-old woman had disregarded pneumaturia since april 1998. in march 1999, computed tomography and barium enema were done to examine anemia and positive fecal occult blood, revealing a left renocolic fistula and bilateral renal stones. intravenous pyelography revealed a left staghorn calculus, non-functioning kidney, and right partial staghorn calculus. urinalysis showed pyuria and the culture grew proteus vulgaris and klebsiella oxytoca. smear and culture of the urine were negative for ac ...	2000	10934611
inhibition of virulence factor expression and swarming differentiation in proteus mirabilis by p-nitrophenylglycerol.	proteus mirabilis is a common cause of upper urinary tract infections that can involve invasion of host urothelial cells. the ability to invade urothelial cells is coupled closely to swarming, a form of multicellular behaviour in which vegetative bacteria differentiate into hyperflagellate, filamentous swarming cells capable of co-ordinated and rapid population migration. co-ordinate expression of virulence factors including urease, protease, haemolysin and flagellin during swarm-cell differenti ...	2000	10933258
mechanisms of action of rabbit cap18 on monolayers and liposomes made from endotoxins or phospholipids.	we have investigated the mechanism of action of the cationic antimicrobial protein (18 kda) cap18 on liposomes and monolayers made from phospholipids and enterobacterial lipopolysaccharides (lps). cap18 intercalates into lipid matrices composed of lps from sensitive strains, weaker into those made of lps from a resistant strain (proteus mirabilis strain r45) or negatively charged phospholipids, but not into those composed of neutral phosphatidylcholine. from the combination of data obtained with ...	2000	10931974
crystallization and preliminary x-ray analysis of chondroitin sulfate abc lyases i and ii from proteus vulgaris.	chondroitin sulfate abc lyases (e.c. 4.2.2.4) are broad-specificity glycosaminoglycan-degrading enzymes. their preferred substrates are chondroitin sulfate and dermatan sulfate, which are broken down to short oligosaccharides. proteus vulgaris produces two such lyases, abc lyase i and ii, with molecular weights of 112-113 kda. diffraction-quality crystals of both enzymes have been obtained by the hanging-drop vapour-diffusion method. abc lyase i crystallizes in space group p2(1)2(1)2(1), with un ...	2000	10930840