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symptomatic diffuse colonic lipomatosis in proteus syndrome. 200111454051
novel class a beta-lactamase sed-1 from citrobacter sedlakii: genetic diversity of beta-lactamases within the citrobacter genus.citrobacter sedlakii 2596, a clinical strain resistant to aminopenicillins, carboxypenicillins, and early cephalosporins such as cephalothin, but remaining susceptible to acylureidopenicillins, carbapenems, and later cephalosporins such as cefotaxime, was isolated from the bile of a patient treated with beta-lactam and quinolone antibiotics. the isolate produced an inducible class a beta-lactamase of pi 8.6, named sed-1, which was purified. characterized by a molecular mass of 30 kda, sed-1 pref ...200111451687
antibacterial and molluscicidal activities of the essential oil of chrysanthemum viscidehirtum.the volatile fraction of chrysanthemum viscidehirtum aerial parts, consisting mainly of limonene, beta-farnesene and many oxygenated sesquiterpenes, was screened for activity against 21 microbial strains. this essential oil exhibited activity against all germs tested, in particular salmonella typhi and proteus mirabilis. it also showed molluscicidal activity against bulinus truncatus.200011449503
structure of the acidic o-specific polysaccharide from proteus vulgaris o39 containing 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic acid.the o-specific polysaccharide of proteus vulgaris o39 was found to contain a new acidic component of proteus lipopolysaccharides, 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic acid (di-n-acetylpseudaminic acid, pse5ac7ac). the following structure of the polysaccharide was determined by nmr spectroscopy, including 2d 1h,(1)h cosy, tocsy, roesy, and 1h,(13)c hmqc experiments, along with selective cleavage of the polysaccharide by solvolysis with anhydrous trifluoromethanesulf ...200111448686
comparative in vitro activity of gemifloxacin, ciprofloxacin, levofloxacin and ofloxacin in a north american surveillance study.the in vitro activity of gemifloxacin, a new fluoroquinolone, was compared to three marketed fluoroquinolones; ciprofloxacin, levofloxacin and ofloxacin against over 4,000 recent clinical isolates covering 29 species isolated in the united states and canada between 1997-1999. based on mic(90)s, gemifloxacin was the most potent fluoroquinolone tested against a majority of gram-positive isolates: streptococcus pneumoniae, penicillin resistant s. pneumoniae, macrolide resistant s. pneumoniae, cipro ...200111448564
[escherichia coli eradication from the blader urine by amoxicillin-sulbactam. intrinsic activity of the inhibitor].at urine concentrations obtained after the oral administration of amoxicillin-sulbactam (500/500 mg) this combination inhibits roughly 90% of escherichia coli and proteus mirabilis strains.200111446908
robotic enzyme amplification: a comparison of some kinetic properties of bovine liver, candida utilis and proteus sp. glutamic dehydrogenases.nadp(h)-specific bakers yeast glucose 6-phosphate dehydrogenase (byg6pdh) was paired, in turn, with each of three different source glutamate dehydrogenases (gdhs): nad(p)-specific bovine liver (blgdh), nadp-specific candida utilis (cugdh) and nadp-specific proteus sp. (psgdh) to constitute three enzyme cycling systems; (i) byg6pdh/blgdh; (ii) byg6pdh/cugdh; and (iii) byg6pdh/psgdh. when incorporated into an enzymatic cycling/amplification system for nad kinase and run on a centrifugal fast analy ...200111445951
identification of the domains of urer, an arac-like transcriptional regulator of the urease gene cluster in proteus mirabilis.proteus mirabilis urease catalyzes the hydrolysis of urea to co(2) and nh(3), resulting in urinary stone formation in individuals with complicated urinary tract infections. urer, a member of the arac family, activates transcription of the genes encoding urease enzyme subunits and accessory proteins, uredabcefg, as well as its own transcription in the presence of urea. based on sequence homology with arac, we hypothesized that urer contains both a dimerization domain and a dna-binding domain. a t ...200111443087
urinary tract infections in women.urinary tract infections (utis) are the most common infections seen in the hospital setting, and the second most common infections seen in the general population. due to women's anatomy, utis are especially problematic for them, and up to one-third of all women will experience a uti at some point during their lifetimes. appropriate treatment of a uti requires accurate classification that includes infection site, complexity of the infection, and the likelihood of recurrence. the predominant patho ...200111442991
longitudinal melanonychia.ungual melanoma is the most serious disease affecting the nail. the majority start with a longitudinal brown streak in the nail.200111442597
urinary tract infections in general practice in a rural community in south trinidad.a prospective study was carried out at a general practice in south trinidad, to assess the sensitivity profiles of urinary isolates in this remote rural community.200111426248
antimicrobial agent resistance in bacterial isolates from patients with diarrhea and urinary tract infection in the sudan.antimicrobial sensitivity tests were performed on four-hundred and ninety-seven bacterial isolates from sudanese patients with diarrhea or urinary tract infections. shigella dysenteriae type i and enteropathogenic escherichia coli showed high resistance rates (percentage of isolates showing antibiotic resistance) against the commonly-used antimicrobial agents: ampicillin, amoxicillin, chloramphenicol, tetracycline, cotrimoxazole, nalidixic acid, sulfonamide, and neomycin, and were completely sen ...200011421374
[increased antibacterial activity of antibiotics with etonium in vitro].the activity of compositions of antibiotics cefasolin, benzylpenicillin and gentamycin with etonium in respect to museum strains staphylococcus aureus 209, escherichia coli k-12, proteus vulgaris 410, p. mirabilis, pseudomonas aeruginosa 19, klebsiella pneumoniae 5054 and polyresistance strains s. aureus, e. coli, p. mirabilis in vitro was researched. the increase of the compositions activity as a result of synergy in the action of their component 4 up to 4496 times has been established.200011421003
the development of the bsac standardized method of disc diffusion testing.the bsac working party on susceptibility testing has developed a standardized method of disc susceptibility testing that has been 'field tested' in 19 diagnostic laboratories in the uk and ireland. the method employs semi-defined media, a semi-confluent inoculum and relates zones of inhibition with bsac-specified mic breakpoints to interpret susceptibility. the bacteria selected for the trial included clinical isolates and control strains from the atcc and nctc national collections. organisms we ...200111420335
proteus syndrome. 200111418739
activity of levofloxacin and ciprofloxacin against urinary pathogens.this study compares the antibacterial activities of levofloxacin and ciprofloxacin against recently isolated urinary tract pathogens, by evaluating their mics and mbcs in accordance with nccls susceptibility tests, time-kill curves and interference with bacterial adhesion to uroepithelial cells. a total of 200 clinical isolates was tested, including the species escherichia coli, klebsiella pneumoniae, proteus mirabilis, proteus vulgaris, providencia rettgeri, pseudomonas aeruginosa, enterococcus ...200111418511
bacterial flora of the conjunctiva and nasal cavity in normal and diseased captive bustards.a survey was carried out to describe the normal aerobic bacterial flora of the conjunctiva and nasal cavity of captive houbara bustards (chlamydotis undulata), kori bustards (ardeotis kori), and white-bellied bustards (eupodotis senegalensis) maintained at the national avian research center, abu dhabi, united arab emirates. a total of 58 samples were examined from the nasal cavity and 55 samples from the conjunctiva of healthy bustards. there was no bacterial growth in 45% of conjunctival sample ...200111417826
a putative insect intracellular endosymbiont stem clade, within the enterobacteriaceae, infered from phylogenetic analysis based on a heterogeneous model of dna evolution.insect intracellular symbiotic bacteria (intracellular endosymbionts, or endocytobionts) were positioned within the gamma 3-proteobacteria using a non-homogeneous model of dna evolution, allowing for rate variability among sites, for gc content heterogeneity among sequences, and applied to a maximum likelihood framework. most of them were found to be closely related within the enterobacteriaceae family, located between proteus and yersinia. these results suggest that such a bacterial group might ...200111411291
biotransformation of crotonobetaine to l(-)-carnitine in proteus sp.two proteins, component i (ci) and component ii (cii), catalyze the biotransformation of crotonobetaine to l(-)-carnitine in proteus sp. ci was purified to electrophoretic homogeneity from cell-free extracts of proteus sp. the n-terminal amino acid sequence of ci showed high similarity (80%) to the caib gene product from escherichia coli o44k74, which encodes the l(-)-carnitine dehydratase. ci alone was unable to convert crotonobetaine into l(-)-carnitine even in the presence of the cosubstrates ...200111409545
tissue antioxidant capacity and bacterial translocation under total parenteral nutrition.alterations in the antioxidative system have been observed during total parenteral nutrition (tpn). light exposure or changes in the composition of tpn formulas may affect this system. bacterial translocation (bt) is frequent under tpn and may be related to oxidative status. the aim of this study was to determine the adverse effects of standard and glutamine-enriched tpn, with or without light exposure, on oxidative status (liver and kidney-reduced glutathione, gsh) and its relationship to bt. t ...200111409162
identification of proteus mirabilis mutants with increased sensitivity to antimicrobial peptides.antimicrobial peptides (aps) are important components of the innate defenses of animals, plants, and microorganisms. however, some bacterial pathogens are resistant to the action of aps. for example, proteus mirabilis is highly resistant to the action of aps, such as polymyxin b (pm), protegrin, and the synthetic protegrin analog ib-367. to better understand this resistance, a transposon mutagenesis approach was used to generate p. mirabilis mutants sensitive to aps. four unique pm-sensitive mut ...200111408219
postantibiotic suppression effect of macrolides on the expression of flagellin in pseudomonas aeruginosa and proteus mirabilis.the phenomenon of postantibiotic effect (pae) encompasses not only the effects of bacterial growth inhibition but also the suppression of virulence factors. we tentatively designated the latter effect the postantibiotic suppression effect (pase). the flagella of gram-negative bacteria are involved in the development of biofilms. we measured the pase of erythromycin (ery) and azithromycin (azm) on the expression of flagellin in pseudomonas aeruginosa and proteus mirabilis. flagellin preparations ...200111406758
[effect of bioceramics on biological activity of microorganisms].biomaterials that come into contact with microorganisms in the middle ear or paranasal sinuses should be tested before clinical application. thus, it is necessary to test the influence exerted on bacterial growth by biomaterials used as bone substitutes in head and neck surgery before implantation.200111405144
presence of escherichia coli o157:h7 in ground beef and ground baby beef meat.a total of 114 beef and baby beef samples were examined. the samples included ground baby beef, mixed ground baby beef and pork, and chopped and shaped meat. the samples were analyzed from 30 different grocery stores in zagreb, croatia. the object of this study was to evaluate the prevalence of escherichia coli o157:h7 in the samples that can enhance the potential risk of outbreaks of hemorrhagic colitis and hemolytic uremic syndrome. the results in all tested samples of e. coli o157:h7 were neg ...200111403139
novel type of fimbriae encoded by the large plasmid of sorbitol-fermenting enterohemorrhagic escherichia coli o157:h(-).sorbitol-fermenting (sf) enterohemorrhagic escherichia coli (ehec) o157:h(-) have emerged as important causes of diarrheal diseases and the hemolytic-uremic syndrome in germany. in this study, we characterized a 32-kb fragment of the plasmid of sf ehec o157:h(-), psfo157, which differs markedly from plasmid po157 of classical non-sorbitol-fermenting ehec o157:h7. we found a cluster of six genes, termed sfpa, sfph, sfpc, sfpd, sfpj, and sfpg, which mediate mannose-resistant hemagglutination and t ...200111401985
the improbable "elephant man". 200111399837
surface sensing, swarmer cell differentiation, and biofilm development. 200111398406
integrin expression, enterocyte maturation, and bacterial internalization.little is known about the molecular mechanisms involved in the translocation of enteric bacteria. adhesion molecules mediate interactions between some enteric pathogens and mammalian cells, but no such interactions have been identified for enterocytes and normal enteric bacteria. using enteric pathogens, adhesion molecule expression has been linked to bacterial internalization and to enterocyte differentiation. therefore, experiments were designed to study enterocyte integrin expression and diff ...200111397127
prior trimethoprim use and trimethoprim-resistant urinary tract infection: a nested case-control study with multivariate analysis for other risk factors.trimethoprim resistance is increasingly prevalent in community-acquired urinary infections. the objective of this study was to evaluate the association between exposure to community-prescribed trimethoprim and other risk factors in subjects and subsequent trimethoprim-resistant urinary tract infection. the design was a nested case-control study using a record-linkage database. study subjects submitted a urine sample to the ninewells hospital laboratory between july 1993 and december 1995. antibi ...200111389110
antibiotic susceptibility of bacterial strains isolated from urinary tract infections in poland.worldwide data show that there is increasing resistance among urinary tract pathogens to conventional drugs. the aim of this study was to obtain data on susceptibility patterns of pathogens responsible for urinary tract infections (utis) in poland to currently used antimicrobial agents. a multicentre study of 141 pathogens from hospital-acquired infections and 460 pathogens from community-acquired infections was carried out between july 1998 and may 1999. the most prevalent aetiological agent wa ...200111389109
[isolation of glutamylendopeptidase precursor from b. licheniformis and its processing in vitro].a secretory system based on l-form cells of proteus mirabilis was developed for production of native bacillus licheniformis glutamylendopeptidase precursor never formerly available. the produced precursor was stable per se under physiological conditions and in presence of trypsin and glutamylendopeptidase from b. intermedius. complete conversion of the precursor to the mature glutamylendopeptidase was performed by bacillar metalloproteases and subtilisin. the artificially processed glutamylendop ...200111385993
phenotypic variability of beta-glucoside utilization and its correlation to pathogenesis process in a few enteric bacteria.utilization of beta-glucosides is markedly variable in the members of the family enterobacteriaceae. the results presented here provide molecular clues for evolutionary events that resulted in the phenotypic variability seen amongst the members of these species. the genomic hybridization of selected enterobacteriaceae members with the escherichia coli bgl and cel genes resulted in detection of a complete homolog of the bgl and cel operons in shigella sonnei, a member that is evolutionarily close ...200111377874
[pattern of antimicrobial susceptibility in uropathogen isolates from children].the bacteriological profile in children with culture-positive bacteriuria was analyzed during a 5-year period. escherichia coli was the most common cause of urinary tract infections (57%), followed by streptococcus faecalis (11%), pseudomonas aeruginosa (8%), and proteus mirabilis (6%). results of antimicrobial susceptibility testing indicated that cephalosporins (first, second and third generation) and nitrofurantoin are the best empirical oral treatment for urinary infections in children. fosf ...200111376352
genetic characterization of the klebsiella pneumoniae waa gene cluster, involved in core lipopolysaccharide biosynthesis.a recombinant cosmid containing genes involved in klebsiella pneumoniae c3 core lipopolysaccharide biosynthesis was identified by its ability to confer bacteriocin 28b resistance to escherichia coli k-12. the recombinant cosmid contains 12 genes, the whole waa gene cluster, flanked by kbl and coad genes, as was found in e. coli k-12. pcr amplification analysis showed that this cluster is conserved in representative k. pneumoniae strains. partial nucleotide sequence determination showed that the ...200111371519
the influence of plasma on the disinfecting activity of the new antimicrobial agent n-chlorotaurine-sodium in comparison with chloramine t.the phenomenon of increasing bactericidal activity of n-chlorotaurine in the presence of chlorine-consuming material has been investigated both on a chemical-analytical and microbiological basis using plasma as substrate and chloramine t for comparison. chlorine consumption assessed by iodometric titration showed a biphasic time-course with a very fast loss of oxidation capacity within one minute (n-chlorotaurine: -9.3%, chloramine t: -16.8%) followed by a slow loss which could still be detected ...200111370708
microbiology of chronic suppurative otitis media with cholesteatoma.to report the result of causative organisms in chronic suppurative otitis media with cholesteatoma.200011369953
the effect of resveratrol on the platelet secretory process induced by endotoxin and thrombin.the effect of resveratrol (trans-3,4',5-trihydroxystilbene) on the release of adenine nucleotides and proteins from blood platelets activated by lipopolysaccharide (lps), from proteus mirabilis and by thrombin, were studied. thrombin stimulated the release of adenine nucleotides from dense granules and proteins from alpha-granules. the lps (0.3 microg/10(8) platelets, 5 min, 37 degrees c), like thrombin (2.5 u/10(8) platelets, 5 min, 37 degrees c) was found to cause a release of adenine nucleoti ...200111368092
[phosphatase from proteus mirabilis].cell-free preparations of proteus mirabilis contained a phosphatase (ec 3.1.3.1), whose activity surpassed that of alkaline phosphatase from escherichia coli. phosphatase was also found in the culture liquid of p. mirabilis. the composition of proteins displaying enzyme activity was assayed by polyacrylamide gel electrophoresis. enzyme synthesis was studied at various stages of bacterial growth. biosynthesis of phosphatase in p. mirabilis (similarly to that in other bacteria) was shown to be ind ...200111357420
fluorogenic assay for penicillin g acylase activity.a simple, highly sensitive, and rapid assay for high-throughput screening of penicillin g acylase-producing bacteria is presented. the method is based on the specific release of fluorescent 7-amino-4-methyl-coumarin through cleavage of phenylacetyl-4-methyl-coumaryl-7-amide by penicillin g acylase. the present method is suitable for screening pure enzymes as well as various penicillin g acylases like those from escherichia coli, proteus rettgeri, and kluyvera citrophila in cell extracts. in addi ...200111355855
structure and serological characterization of an nepsilon-[(r)-1-carboxyethyl]-l-lysine-containing o-chain of the lipopolysaccharide of proteus mirabilis o13.in this paper we present the structure and describe serological properties of the o-specific polysaccharide of proteus mirabilis o13 lipopolysaccharide, which contains a unique component: an amide of d-galacturonic acid (d-gala) with an unusual amino acid, nepsilon-[(r)-1-carboxyethyl]-l-lysine (alaninolysine, alalys). selective chemical degradations of either gala or alalys resulted in the loss of the serological reactivity of the polysaccharide with anti-o serum against p. mirabilis o13. neith ...200111348021
proteus mirabilis septic monoarthritis in a patient with cpdd. 200111344817
leukocyte antimicrobial function in patients with leprosy.patients with lepromatous leprosy are unresponsive to lepromin skin-test material and possess defective lymphocyte function in vitro, including impaired mitogenesis in response to antigens of mycobacterium leprae. it has been claimed that their macrophages cannot digest m. leprae in vitro; such a defect could explain both lepromin nonreactivity and impaired lymphocyte function on the basis of failure of the afferent limb of the immune response (i.e., defective macrophage "processing" of m. lepra ...197411344550
causes of premature death in proteus syndrome. 200111343327
[comparative study of the effects of different sour milk products on the intestinal microflora of healthy infants].our study was aimed to investigate in comparable conditions the composition of intestinal microflora of healthy infants who were given 9 types of fermented milk products. there were observed 103 practically healthy infants aged from 4 month to 1,5 years old. the obtained data have shown that studied products improve intestinal microflora in infants. significant increase of bifidobacteria in infants intestines was promoted by fermented milk products containing alive bifidobacteria with rather hig ...200111338340
antibiotic selective pressure and development of bacterial resistance.this study evaluates the development of resistance in gram-negative rods to cefotaxime and ceftazidime, ofloxacin and ciprofloxacin, gentamicin and amikacin, meropenem and ampicillin/sulbactam over a five year period of use (1994-1998) at the university hospital in olomouc, czech republic. the development of bacterial resistance was linked with antibiotic use and hence selective pressure which was specific for the type of antibiotic and the bacterial species. statistically significant correlatio ...200111337221
transferable antibiotic resistance in multiresistant nosocomial acinetobacter baumannii strains from seven clinics in the slovak and czech republics.sixty-seven multiresistant nosocomial acinetobacter baumannii isolates from patients hospitalized mostly in intensive care units of seven clinics in slovak and czech republic were tested to determine their ability to transfer antibiotic resistance. all isolates were resistant to kanamycin, ticarcillin, cephalothin, cefotaxime, ceftazidime, aztreonam and susceptible to carbapenems, sulbactam and ampicillin/sulbactam. sixty-five out of 67 strains transferred resistance determinants to escherichia ...200111330360
16s rdna-based identification of bacteria from conjunctival swabs by pcr and dgge fingerprinting.establishment of a new molecular biology technique for the identification of multiple bacteria from the ocular environment, which can be applied supplementarily to cultivation in cases of severe bacterial infections.200111328723
evidence of in vivo transfer of a plasmid encoding the extended-spectrum beta-lactamase tem-24 and other resistance factors among different members of the family enterobacteriaceae.the epidemiological study of several multidrug-resistant enterobacteriaceae isolated from five patients demonstrated in vivo dissemination of a 100-kb plasmid encoding the extended-spectrum beta-lactamase tem-24 from a clonal strain of enterobacter aerogenes to different strains of klebsiella pneumoniae, escherichia coli, proteus vulgaris, proteus mirabilis, and serratia marcescens.200111326030
the multifaceted challenges of proteus syndrome.proteus syndrome is a rare and sporadic disorder that causes postnatal overgrowth of multiple tissues in a mosaic pattern. the overgrowth can involve skin, subcutaneous tissue, connective tissue (including bone), the central nervous system, and viscera. complications of proteus syndrome include, among others, progressive skeletal deformities, invasive lipomas, benign and malignant tumors, and deep venous thrombosis with pulmonary embolism. care of patients with proteus syndrome presents signific ...200111325326
structure of the o-specific polysaccharide of proteus mirabilis o16 containing ethanolamine phosphate and ribitol phosphate.the o-specific polysaccharide of proteus mirabilis o16 was studied by 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, noesy, h-detected 1h,13c hmqc, hmqc-tocsy, and 1h,31p hmqc experiments, along with chemical methods. the polysaccharide was found to be a ribitol teichoic acid-like polymer having the following structure [structure: see text].200111322736
structure of the o-specific polysaccharide of proteus vulgaris o4 containing a new component of bacterial polysaccharides, 4,6-dideoxy-4.a high-molecular-mass o-specific polysaccharide was obtained by mild acid degradation of proteus vulgaris o4 lipopolysaccharide followed by gpc. the polysaccharide was studied by chemical methods along with 1h and 13c nmr spectroscopy, including two-dimensional cosy, tocsy, noesy, h-detected 1h,13c hmqc, and 1h,13c hmbc experiments. solvolysis of the polysaccharide with trifluoromethanesulfonic (triflic) acid resulted in a glcpa-(1 --> 3)-glcnac disaccharide and a novel amino sugar derivative, 4 ...200111322733
variations in the prevalence of strains expressing an extended-spectrum beta-lactamase phenotype and characterization of isolates from europe, the americas, and the western pacific region.to evaluate the prevalence of extended-spectrum beta-lactamase (esbl)-producing strains among species of enterobacteriaceae, a microdilution susceptibility test was performed with strains of klebsiella pneumoniae, escherichia coli, proteus mirabilis, and salmonella species that were isolated as part of the sentry project. the highest percentage of esbl phenotype (defined as a minimum inhibitory concentration [mic] > or =2 microg/ml for ceftazidime, ceftriaxone, or aztreonam) was detected among k ...200111320450
[new pyrrolo-pyrimidine derivatives with antifungal or antibacterial properties in vitro]. 200111320339
proteus syndrome: a variant with eye involvement. 200111318277
[xanthogranulomatous pyelonephritis. our experience and review of the literature].ten cases of xanthogranulomatous pyelonephritis (xgp) with diagnostic and therapeutic observation are described and other experiences reported in the literature are analyzed.200011315325
[antimicrobial activity of ciprofloxacin and netilmicin compared to various antibiotics in lome, togo].we report the antimicrobial activity of ciprofloxacin and netilmicin on 577 strains such as s. aureus, pseudomonas, e. coli, salmonella, proteus, klebsiella and enterobacter. isolation and identification were performed by standard methods. disk diffusion tests were performed to evalute the susceptibility. the percentage resistance to ciprofloxacin for bacteria was: e. coli = 15%, enterobacter = 13%, proteus = 10%, pseudomonas = 9%, s. aureus and klebsiella = 4%. the percentage resistance to neti ...200111313234
zentralblatt für bakteriologie--100 years ago: agglutination and filament formation of proteus bacteria and maternal-fetal transfer of agglutinins. 200111310442
theory of periodic swarming of bacteria: application to proteus mirabilis.the periodic swarming of bacteria is one of the simplest examples for pattern formation produced by the self-organized collective behavior of a large number of organisms. in the spectacular colonies of proteus mirabilis (the most common species exhibiting this type of growth), a series of concentric rings are developed as the bacteria multiply and swarm following a scenario that periodically repeats itself. we have developed a theoretical description for this process in order to obtain a deeper ...200111308686
analytic model for ring pattern formation by bacterial swarmers.we analyze a model proposed by medvedev, kaper, and kopell (the mkk model) for ring formation in two-dimensional bacterial colonies of proteus mirabilis. we correct the model to formally include a feature crucial of the ring generation mechanism: a bacterial density threshold to the nonlinear diffusivity of the mkk model. we numerically integrate the model equations, and observe the logarithmic profiles of the bacterial densities near the front. these lead us to define a consolidation front dist ...200111308679
pemphigus vulgaris and disseminated nocardiosis.infectious diseases, in particular septicaemia from staphylococcus aureus, proteus vulgaris and pseudomonas aeruginosa, are the most severe and frequent complications for the immunosuppressive therapy of pemphigus. infection by nocardia asteroides in subjects with pemphigus vulgaris is rare. we report the sixth case found of such an association; the subject died of disseminated nocardiosis while receiving steroids and immunosuppressive drugs, 4 years after being diagnosed with chronic pemphigus ...200011305389
intradermal skin test with diabetes specific antigens in patients with type 1 diabetes.cell mediated immune response in vitro to a number of antigens has been reported in patients with type 1 diabetes. the aim of the present study was to develop an in vivo intradermal (delayed type hypersensitivity) skin test using antigens known to be recognized by lymphocytes of patients with type 1 diabetes and to compare, where possible, the in vivo response to the in vitro t cell proliferation to the same antigens. the skin test was performed in the following group of patients: 55 with recent ...200111298123
xanthogranulomatous pyelonephritis in childhood.demographic data, clinical presentation, associated abnormalities, and radiologic findings were evaluated to outline diagnostic criteria that may lead to the diagnosis of xanthogranulomatous pyelonephritis in children.200111283885
biochemical identification and characterization of dna groups within the proteus vulgaris complex.we placed 43 isolates belonging to the proteus vulgaris complex into proposed dna groups (genomovars) using five previously recommended tests (tests for esculin hydrolysis, production of acid from salicin, l-rhamnose fermentation, and elaboration of dnase and lipase). on the basis of the results of these five tests, 49% of the isolates fell into dna groups 5 and 6, 37% fell into dna group 2, and the remaining 14% fell into dna groups 3 and 4. sequencing of the 16s rrna genes of 11 members of dna ...200111283033
biodiversity and horizontal gene transfer in culturable bacteria isolated from activated sludge enriched in nonylphenol ethoxylates.one hundred and twenty bacterial isolates, from activated sludge of a treatment plant collecting wastes enriched in ethoxylated nonylphenols, were studied. sixty isolates were selected on rich medium and 60 on mineral medium containing two nonylphenol ethoxylates as the sole carbon source. analysis of biodiversity at the species level was performed by comparing the alui restriction patterns of the 16s ribosomal dna amplified by pcr from 120 isolates. the rdna restriction analysis enabled us to c ...200111281319
the structure of the carbohydrate backbone of the core-lipid a region of the lipopolysaccharide from proteus penneri strain 40: new proteus strains containing open-chain acetal-linked n-acetylgalactosamine in the core part of the lps.analysis of the core part of the lps from several strains of proteus revealed that p. penneri strains 2, 11, 19, 107, and p. vulgaris serotypes 04 and 08 have the same structure with a new type of linkage between monosaccharidesan open-chain acetal--that was previously determined for p. vulgaris ox2 and p. penneri 17. the lps from p. penneri strain 40 contains the same structure substituted with one additional monosaccharide: [molecular structure: see text] where (1s)-galanac1 is a residue of n- ...200111269407
synthesis of mannich bases of some 2,5-disubstituted 4-thiazolidinones and evaluation of their antimicrobial activities.5-phenyl/methyl-5-morpholinomethyl/pyrrolidinomethyl-2-(5- aryl-1,3,4-oxadiazol-2-yl)imino]-4-thiazolidinones (5a-m) were synthesized by the reaction of 5-phenyl/methyl-2-[(5-aryl-1,3,4-oxadiazol -2-yl)imino]-4-thiazolidinones (4a-j) with formaldehyde and morpholine or pyrrolidine. the structures of the compounds were determined by analytical and spectral (ir, 1h-nmr, eims) methods. the antibacterial activities of the novel compounds against staphylococcus aureus atcc 6538, staphylococcus epider ...200111268772
antimicrobial activity of honokiol and magnolol isolated from magnolia officinalis.the antimicrobial activity of honokiol and magnolol, the main constituents of magnolia officinalis was investigated. the antimicrobial activity was assayed by the agar dilution method using brain heart infusion medium and the minimum inhibitory concentration (mic) were determined for each compound using a twofold serial dilution assay. the results showed that honokiol and magnolol have a marked antimicrobial effect (mic = 25 microg/ml) against actinobacillus actinomycetemcomitans, porphyromonas ...200111268114
differentiation of bacterial autolysins by zymogram analysis.the use of zymograms in which the bacterial cell wall heteropolymer peptidoglycan is incorporated into the resolving gel of sds-page has led to the identification of various sds stable peptidoglycan hydrolases (autolysins). to examine the specificity of autolysins with respect to o-acetylated peptidoglycan, a discontinuous sds-page system has been developed that operates under neutral conditions. [bis(2-hydroxyethyl)imino]tris(hydroxymethyl)methane (bis-tris) buffers are employed with ph 6.8 and ...200111262168
comparison of the pr mutant with the wild-type strain of proteus mirabilis brings insight into peroxide resistance factors and regulation of catalase expression.the peroxide resistant mutant (pr) of proteus mirabilis was characterized by an increased constitutive catalase activity concomitant with a large production of specific mrna. survival toward hydrogen peroxide during exponential phase was increased by h2o2 pretreatment in the wild type but not in the mutant, although the catalase of both strains was not inducible under these conditions. in the mutant, besides catalase, over-produced proteins comprised two different alkyl hydroperoxide reductase s ...200111261492
a new bio-active flavonol glycoside from the stems of butea superba roxb.a new bio-active flavonol glycoside was isolated from the stems of butea superba roxb, and its structure was determined by spectral analysis and chemical degradations as 3,5,7,3',4'-pentahydroxy-8-methoxy-flavonol-3-o-beta-d-xylopyranosyl(1-->2) -alpha-l-rhamnopyranoside. the compound 1 showed antimicrobial activity against plant pathogenic fungi trich viride, asprgillus fumigatus, a. niger, a. terreus, penicillium expansum, helmitnhosporium oryzae, botxitis cinerea, rhizopus oligosporus, r. chi ...199811261215
bladder washouts in the management of long-term catheters.it has been estimated that 40-50% of patients with long-term catheters can suffer with catheter blockage. this not only causes distress to the patient but also increases the demands on community nurses' time and resources. infection with bacteria such as proteus causes the urine to become alkaline. crystalline deposits can then form inside the catheter lumen which causes blockage. nurses often manage blocked catheters with the use of bladder 'washouts' or bladder instillations. however, the lite ...200011261025
interaction of bladder glycoprotein gp51 with uropathogenic bacteria.a major component of bladder surface mucin is a glycoprotein gp51 (molecular weight 51 kd.). gp51, which has previously been isolated from rabbit mucosa, appears to function as part of the defense mechanism in an in vivo infection model. gp51 coats the epithelium and is secreted into the urine, as detected by immunohistochemical testing and enzyme-linked immunosorbent assay (elisa). increased urinary gp51 occurs during urinary tract infection. to elucidate the role of gp51 as a component of the ...200111257713
epigenetic regulation of carnitine metabolising enzymes in proteus sp. under aerobic conditions.proteus sp. is able to catalyse the reversible transformation of crotonobetaine into l(-)-carnitine during aerobic growth. contrary to other enterobacteriaceae no reduction of crotonobetaine into gamma-butyrobetaine could be detected in the culture supernatants. activities of l(-)-carnitine dehydratase, carnitine racemasing system and crotonobetaine reductase could be determined enzymatically in cell-free extracts of proteus sp. small amounts of gamma-butyrobetaine were found in cell-free extrac ...200111257539
new tem variant (tem-92) produced by proteus mirabilis and providencia stuartii isolates.the sequences of the bla(tem) genes encoding tem-92 in proteus mirabilis and providencia stuartii isolates were determined and were found to be identical. except for positions 218 (lys-6) and 512 (lys-104), the nucleotide sequence of bla(tem-92) was identical to that of bla(tem-20), including the sequence of the promoter region harboring a 135-bp deletion combined with a g-162-->t substitution. the deduced amino acid sequence of tem-92 differed from that of tem-52 by the presence of a substituti ...200111257046
investigation on the nutritive value and microbiological quality of wild quail carcasses.quail meats have many advantages and superiority one the other species of poultry. this study was planned to throw plenty of light on gross chemical composition, lipid fractions, fatty acids composition, amino acids composition, of thigh and breast of male and female wild quail meat as well as the microbiological quality. the mean values of moisture, protein, fat, ash and energy contents ranged from 60.1 to 69.2%, 55.0 to 68.8%, 28.8 to 42.1%, 2.40 to 3.63% and 696 to 1000 kj, respectively. seve ...200111253642
[the role of urinary tract infections in burn patients].patients with burns are predisposed to a variety of infectious complications, urinary tract infections inclusive. it is the aim of the study to assay the incidence rate, etiology and antibacterial sensitivity of the leading pathogens causing bacteriuria in burnt patients, and compare them with the respective characteristics of the pathogens causing wound infection in the same contingent of patients, and urinary tract infection in uro-nephrological patients. over a five-year period, a total of 24 ...199811247066
chronic osteomyelitis: clarification of nuclear medicine findings by fusion scans. 200111245118
lactobacillus plantarum reduces infection of pancreatic necrosis in experimental acute pancreatitis.infection is the commonest cause of death in acute pancreatitis. early reduction of commensal flora (particularly lactobacillus species) and, at the same time, overgrowth of enterobacteriaceae, especially escherichia coli, have recently been described during acute pancreatitis. lactobacillus plantarum has been shown to be effective in reducing the egress of endotoxin and microbial translocation in several experimental models such as chemically induced hepatitis and ulcerative colitis.200111244259
bacterial protease ecp32 specifically hydrolyzing actin and its effect on cytoskeleton in vivo.a procedure for isolation of bacterial protease ecp32 yielding 100 microg of the enzyme from 10 liters of the escherichia coli strain a2 liquid culture has been developed. the procedure includes chromatography, ultrafiltration, and page under non-denaturing conditions. the purified preparation contained about 80% ecp32 and did not exhibit atpase activity. polyclonal ecp32-specific antibodies have been produced, and a two-stage procedure for the isolation of protease ecp32 involving affinity chro ...200111240398
immunocytochemical demonstration of visual pigments in the degenerate retinal and pineal photoreceptors of the blind cave salamander (proteus anguinus).visual pigments in the regressed eye and pineal of the depigmented neotenic urodele, the blind cave salamander (proteus anguinus anguinus), were studied by immunocytochemistry with anti-opsin antibodies. the study included light- and electron-microscopic investigations of both the eye and the pineal organ. a comparison was made with the black pigmented subspecies proteus anguinus parkelj (black proteus), which has a normal eye structure. in the retina of the black proteus, we found principal rod ...200111236001
[antibacterial activity of urine after administration of ofloxacin for 5 days].the antibacterial activity of ofloxacin was evaluated in urine over a period of 96 h after oral administration for 5 days of 200 mg twice a day in 12 healthy female volunteers. bacteriostatic and bactericidal activity of urines were studied for five strains of enterobacterias recovered from urinary infections: two strains of escherichia coli nal-s and nal-r, two strains of proteus mirabilis nal-s and nal-r, and one strain of klebsiella pneumoniae nal-s. mean urinary concentrations of ofloxacin w ...200011234464
[endonuclease from proteus mirabilis].two isoforms of nuclease displaying dnase and rnase activities were found in the culture liquid and periplasm of proteus mirabilis. the enzyme was isolated from the periplasm and then purified to a functionally homogeneous state. the nuclease was equally potent in cleaving denatured and native dnas by the endonuclease mechanism and was designated pm endonuclease. the endonuclease was shown to be a temperature-dependent enzyme with a ph optimum of 10.4-10.6, requiring the presence of bivalent met ...200111234403
effect of subclinical uterine infection on cervical and uterine involution, estrous activity and fertility in postpartum buffaloes.nili-ravi buffaloes (n=29) that calved normally between august and november and did not develop any clinical reproductive disorder after calving were studied for the incidence of sub-clinical bacterial infection of the uterus and its effects on postpartum reproductive efficiency. the incidence of subclinical uterine infection was 24% (7/29). involution of the cervix and uterus was slower (p < 0.01) in the infected group than in the normal group (45.6 vs 31.1 days and 46.3 vs 35.8 days), respecti ...200111233783
physiological and behavioral responses of stonefly nymphs to enhanced limestone treatment of acid mine drainage.a new acid mine drainage (amd) treatment system uses pulsed, fluidized beds of limestone, and carbon dioxide pretreatment of influent amd, to enhance limestone neutralization of amd. we conducted laboratory studies to evaluate the behavior and physiology of larval stoneflies (pteronarcys proteus, plecoptera) exposed to effluents produced by the treatment system. survival, sodium balance, drift, and feeding responses by p. proteus to treated and untreated amd were examined. p. proteus nymphs exhi ...200111228958
[efficacy of bacteriophages in complex treatment of patients with burn wounds].results of clinical and laboratory evaluation of the treatment with pyobacteriophage in tablets of the patients with burn wounds are presented. it was shown that phagotherapy provided more rapid cure of pyoseptic complications, temperature normalization, wounds purification and lower lethality bacteriological analysis of wound secretions revealed that after the treatment staphylococci and streptococci were cultured 2 times rarely, proteus spp. were isolated 1.5 times rarely, e. coli was not isol ...200111221078
an intelligent rapid odour recognition model in discrimination of helicobacter pylori and other gastroesophageal isolates in vitro.two series of experiments are reported which result in the discrimination between helicobacter pylori and other bacterial gastroesophageal isolates using a newly developed odour generating system, an electronic nose and a hybrid intelligent odour recognition system. in the first series of experiments, after 5 h of growth (37 degrees c), 53 volatile 'sniffs' were collected over the headspace of complex broth cultures of the following clinical isolates: staphylococcus aureus, klebsiella sp., h. py ...200011219746
[xanthogranulomatous pyelonephritis with fistula in the gluteal region]. 200011219004
[electrophoretic forms of glucose-6-phosphate dehydrogenase, acid phosphatase and esterase in amoeba species amoebas].glucose-6-phosphate dehydrogenase (g6pd), acid phosphatase and esterases in free-living amoebae of 7 amoeba species were investigated with the use of disc-electrophoresis in polyacrylamide gel. the evidence provided is suggestive that the electrophoretic isoenzyme patterns of acid phosphatase and esterases (and g6pd in some cases), in addition to a few morphological characters, can serve as a taxonomic criterion for species identification within this genus, as well as for revealing erroneously c ...200011213727
[clinical and laboratory efficacy of the novel difluoroquinolone sparflo (sparfloxacin) in therapy of skin and soft tissue infections].results of clinical trial of new difluoroquinolone--sparflo (sparfloxacin, dr. reddy's laboratories ltd) are presented. sparfloxacin was used in the treatment of 24 patients at the department of wounds and wounds infections (11 patients) and at the department of burn wounds (13 patients) of the a.v. vishnevsky institute of surgery. after the treatment with sparfloxacin pathogen eradication was stated in 18 patients, eradication with superinfection--in 13 patients, persistence--in 3 patients. thu ...200011212495
bacteriological study of pus isolates from neuropathic plantar ulcers associated with acute inflammatory phase.in this retrospective study, sensitivity of organisms cultured from ulcers of leprosy patients without and with diabetes mellitus, diabetic patients without leprosy and patients with ulcers from other causes was examined. the profile of organisms grown from these groups of patients did not differ significantly. however, there was a high prevalence of organisms like proteus, e. coli and enterococcus in the ulcers of leprosy patients indicating faecal contamination of the ulcers. co-trimaxazole an ...200011212478
[diagnostic value of a novel nutrient medium for isolation and cultivation of pathogens causing enteric yersiniosis and pseudotuberculosis].a new nutrient medium for isolation and cultivation of the causative agents of enteric yersiniosis and pseudotuberculosis was found to have advantages over endo medium in its differentiating and inhibiting properties. this medium permitted the easy differentiation of yersinia pseudotuberculosis from y. enterocolitica, as well as from escherichia coli, shigella flexneri, klebsiella pneumoniae, k. rhinoscleromatis, hafnia, enterobacter and citrobacter by color; from proteus inconstans by swarming. ...200011210625
proteus igg antibodies and c-reactive protein in english, norwegian and spanish patients with rheumatoid arthritis.the distribution of proteus antibody levels was compared in english, norwegian and spanish patients with rheumatoid arthritis (ra). using an indirect immunofluorescence method, the igg antibody titre against proteus mirabilis was measured in the sera of 27 english, 53 norwegian and 34 spanish patients with ra and divided into active and inactive disease groups according to the serum c-reactive protein (crp) level (> or = 10 mg/l). serum samples were also collected from 25 english, 30 norwegian a ...199911206342
[bacteriologic evaluation of otitis externa and chronic otitis media].the authors present results of bacteriological examinations in 187 patients with chronic otitis media (omc) and otitis externa treated in ent department, medical university of gdaƄsk in the years 1997-1998. ear swabs and aspirates were investigated for aerobes and anaerobes 254 bacterial isolates were obtained. among 150 patients with omc aerobes were isolated in 130 (86.7%) in 13 (8.5%) mixed aero-anaerobes flore and in 20 (19.3%) no bacterial growth was observed. from patients with com there w ...200011202342
[biosynthesis of proteus mirabilis nuclease].the culture liquid and periplasm of proteus mirabilis contained nuclease, an enzyme with dnase and rnase activities. the nuclease was most actively synthesized in the early exponential and stationary growth phases. nuclease synthesis was regulated by nucleic acids (induction by substrate) and inorganic phosphate (end-product inhibition). the synthesis and secretion of nuclease by p. mirabilis was induced by mitomycin c, an inducer of the sos functions of cells. this suggests the involvement of s ...200011195576
a survey of gram-negative bacteria survival on hospital fabrics and plastics.one critical factor for the transmission of microorganisms from person to person or from the environment to a person (patient or health care worker) is the ability of the microbe to survive on an environmental surface. the purpose of this study was to determine the length of survival of various gram-negative bacteria on fabrics and plastics commonly used in hospitals. seven materials were tested: smooth cotton (clothing), cotton terry (towels), 60% cotton-40% polyester blend (scrub suits and lab ...200011194806
remaining small bowel length: association with catheter sepsis in patients receiving home total parenteral nutrition: evidence of bacterial translocation.patients with short bowel syndrome (sbs) receiving total parenteral nutrition (tpn) have a high incidence of catheter-related sepsis, one of its major complications. the aim of this study was to correlate the length of remaining small bowel (rsb) with septic episodes related to the central venous catheter in a group of patients with severe sbs with home tpn. the length of the rsb (<50 cm or > or = 50 cm) was related to the frequency of catheter sepsis, time until the first episode, and the agent ...200011193720
[clinical experiences of endotoxin removal columns in septic shock due to urosepsis: report of three cases].clinical experiences of endotoxin removal using polymyxin b immobilized fiber column (pmx) in three septic shock patients are reported. the patients were referred to our hospital with complaints of high fever and severe pain during urination. physical examination and laboratory data revealed severe hypotension, tachycardia, and pyuria. diagnoses of septic shock due to urosepsis were made. after antibiotic therapy and anti-shock therapy, direct hemoperfusion using polymyxin b immobilized fiber co ...200011193305
[treatment of pressure sores accompanied by infection in outpatients with spinal cord injury].the occurrence of infection at pressure sore sites due to multiple drug resistant microbes not only delays healing at the time of infection, but it can become a major factor preventing an acceptable level of adl (activities of daily living) in the home. we studied a method for treating infection in pressure sores in spinal cord injury patients living at home who were commuting to our hospital for treatment. after establishing drug sensitivity in all patients, we instructed patients and their fam ...200011190341
proteus: the debate concerning the prevention of infectious disease transmission by male circumcision. 200011183378
in vitro and in vivo properties of ro 63-9141, a novel broad-spectrum cephalosporin with activity against methicillin-resistant staphylococci.ro 63-9141 is a new member of the pyrrolidinone-3-ylidenemethyl cephem series of cephalosporins. its antibacterial spectrum was evaluated against significant gram-positive and gram-negative pathogens in comparison with those of reference drugs, including cefotaxime, cefepime, meropenem, and ciprofloxacin. ro 63-9141 showed high antibacterial in vitro activity against gram-positive bacteria except ampicillin-resistant enterococci, particularly vancomycin-resistant strains of enterococcus faecium. ...200111181368
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