Publications

TitleAbstractYear
Filter
PMID(sorted descending)
Filter
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (2000). ii. background of patients].five-hundred eighty eight bacterial strains isolated from 435 patients diagnosed as having urinary tract infections (utis) in 10 institutions in japan were supplied between august 2000 and july 2001. then, the clinical background of patients were investigated such as sex, age, and type of infections, infections and kind of bacteria, frequency of bacteria isolation by age and infections, bacteria and infections by timing of antibiotics administration, and bacteria and infections by surgical proce ...200212378870
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (2000). i. susceptibility distribution].the bacterial strains isolated from patients diagnosed as having urinary tract infections (utis) in 10 institutions in japan were supplied between the period of august 2000 and july 2001. then, the susceptibilities of them to many kinds of antimicrobial agents were investigated. the number of them were 511 strains. the breakdown of these strains was gram-positive bacteria as 29.0% and gram-negative bacteria as 71.0%. susceptibilities of these bacteria to antimicrobial agents were as follows; van ...200212378869
outcome of percutaneous nephrostomy for the management of pyonephrosis.the aim of this study was to evaluate the efficacy of percutaneous nephrostomy (pcn) drainage for the interim management of pyonephrosis.200212376218
venous sinus thrombosis after proteus vulgaris meningitis and concomitant clostridium abscess formation.a 19-y-old woman presented with proteus vulgaris meningitis as a complication of chronic otitis media. despite treatment with ceftazidime and amikacin no clinical improvement was observed. cranial mri revealed right-sided mastoiditis/otitis media and venous sinus thrombosis. after mastoidectomy, repeat cranial mri demonstrated abscess formation in the venous sinuses. the abscess was drained. clostridium spp. was isolated from the abscess culture.200212374367
[use of mpo chromogenic culture medium for routine processing of urine cultures].the chromogenic culture medium, mpo, was compared to culture on cled (cystein, lactose, electrolyte-deficient) agar for the detection, enumeration and identification of urinary tract pathogens.200212372235
revisiting proteus: do minor changes in lectin structure matter in biological activity? lessons from and potential biotechnological uses of the diocleinae subtribe lectins.significant differences in function have been observed among lectins structurally similar to concanavalin a, but their high homology with this widely used lectin has kept them in obscurity. the observation of large differences in the potency of many of these diocleinae lectins as stimulators of interferon-g production by human peripheral blood mononuclear cells has lead to a major effort to unravel their chemical structure and biological activity. modeling studies of some of these lectins reveal ...200112370020
serratia type pore forming toxins.the serratia marcescens hemolysin represents a new type of hemolysin and has been studied in great molecular detail with regard to structure, activation and secretion. it has nothing in common with the pore forming toxins of e. coli type (rtx toxins), the staphylococcus aureus alpha-toxin or the thiol activated toxin of group a beta-hemolytic streptococci (streptolysin o). studies on erythrocytes, eukaryotic cells and artificial black lipid membranes, have shown that the mechanism of pore format ...200012369921
proteinases of common pathogenic bacteria degrade and inactivate the antibacterial peptide ll-37.effectors of the innate immune system, the anti-bacterial peptides, have pivotal roles in preventing infection at epithelial surfaces. here we show that proteinases of the significant human pathogens pseudomonas aeruginosa, enterococcus faecalis, proteus mirabilis and streptococcus pyogenes, degrade the antibacterial peptide ll-37. analysis by mass spectrometry of fragments generated by p. aeruginosa elastase in vitro revealed that the initial cleavages occurred at asn-leu and asp-phe, followed ...200212366839
rapid detection of obesumbacterium proteus from yeast and wort using polymerase chain reaction.to design and evaluate pcr primers for the rapid detection of obesumbacterium proteus.200212358688
structure of the o-specific polysaccharide of proteus penneri 103 containing ribitol and 2-aminoethanol phosphates.the o-specific polysaccharide of the lipopolysaccharide of proteus penneri strain 103 was studied using 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, noesy, h-detected 1h,(13)c hmqc, 1h, 31p hmqc, and hmbc experiments. it was found that the polysaccharide is built up of oligosaccharide-ribitol phosphate repeating units and thus resembles ribitol teichoic acids of gram-positive bacteria. the following structure of the polysaccharide was established:where etn and rib-ol are ethanolamine a ...200212350322
[a comparative study of the various organisms causing salpingitis and their potential presence in iud wearers].a prospective study was undertaken to compare the enbdometrial and vaginal bacteria of a continuous series of 283 patients who had iuds removed between june 1, 1979 and june 1, 1983, by the same clinician. measures were taken to avoid contamination of the iud upon removal by the vaginal bacteria, but as a practical matter it was impossible to avoid all contact. the iuds involved were multiload copper 250 or 375, gyne t and t 200, nova t, and dimelys. the number of positive cultures from the ...198512339972
[microbiological screening and postoperative course in patients undergoing first trimester abortion].pelvic infections are a serious and widespread gynecological disease, often leading to sterility. procedures like evacuation of the products of conception by vacuum suction are known to facilitate their appearance. 300 patients were examined the same day of the abortion (first trimester); before the hysterosuction a vaginal tampon was taken from the cervix and from the posterior fornix. the patients were invited to come back for an exam in two weeks and to refer to the clinic for any problem ...198512315928
honey: its antibacterial action in the treatment of gastroenteritis.the results of several recent "in vitro" studies using honey in oral rehydration solutions (ors) givien to infants and children with gastroenteritis showed that the use of honey had caused a reduction in the duration of bacterial diarrhea, and it had worked well as a substitute for glucose in an oral rehydration solution containing electrolytes. studies have confirmed that honey shortens the duration of diarrhea in patients with bacterial gastroenteritis through its antibacterial properties. i ...198512314387
[etiopathogenic and diagnostic aspects of postabortion peritonitis].the causes, diagnosis, and treatment of postabortion peritonitis are discussed with reference to 85 cases, analyzing the causal agents and their sensitivity to various antibiotic treatments, and classifying them by frequency and clinical symptoms. the following germs were found in the patients: staphylococcus hemolyticus, esch. coli, pseudomonas, proteus, procyaneus, and enterococcus, which showed different responses to the various antibiotics used. the importance of a correct diagnosis of th ...197512307372
structural requirements of the mrna for intracistronic translation initiation of the enterobacterial infb gene.the gene infb encodes the prokaryotic translation initiation factor if2, a central macromolecular component in the formation of the ribosomal 70s initiation complex. in escherichia coli, infb encodes three forms of if2: if2alpha, if2beta and if2gamma. the expression of if2beta and if2gamma is a tandem translation from intact infb mrna and not merely a translation of post-transcriptionally truncated mrna. the molecular mechanism responsible for the ribosomal recognition of the two intracistronic ...200212296821
coping with osteomyelitis.osteomyelitis, or infection of the bones, is a common orthopedic condition in the young in developing countries which causes severe deformity and morbidity. acute osteomyelitis is of hematogenous origin, and in adults it results from infected open fractures or a leg ulcer from staphylococcus aureus (over 80%), or mixed with streptococcus pyogenes, pseudomonas spp, proteus spp, escherichia coli, and salmonella typhi. in a classic case of acute osteomyelitis, a child under 10 presents severe bon ...199212285913
bacteriological study of extracted iucds.previous studies have shown the users of intrauterine devices have a 4.4 times greater risk of pelvic inflammatory disease. this present study intends to categorize the distribution of microbes in cultures from different types of extracted copper intrauterine devices (iucds). bacteriological studies of aerobic and anaerobic organisms were performed on the extracted iucds of 522 iucd users; 480 wearing a multiload cu 250, 15 wearing a copper t, 22 lippes loop and 5 a copper 7. cultures were ne ...198912283073
neonatal proteus syndrome? 200212244562
use of an electronic nose system for diagnoses of urinary tract infections.the use of volatile production patterns produced by bacterial contaminants in urine samples were examined using electronic nose technology. in two experiments 25 and 45 samples from patients were analysed for specific bacterial contaminants using agar culture techniques and the major uti bacterial species identified. these samples were also analysed by incubation in a volatile generation test tube system for 4-5 h. the volatile production patterns were then analysed using an electronic nose syst ...200212243908
antimicrobial and anti-inflammatory activity of folklore: mallotus peltatus leaf extract.since ages mallotus peltatus (geist) muell. arg. var acuminatus (euphorbiaceae) leaf and stem bark is used in folk medicine to cure intestinal ailments and skin infections. in several intestinal ailments, localized inflammation is of common occurrence and hence we have evaluated the antimicrobial as well as anti-inflammatory activity of m. peltatus leaf extract. the crude methanol extract of m. peltatus leaves was found to be active against staphylococcus aureus, staphylococcus saprophyticus, st ...200212242000
pathogenic effects of bacteria isolated from larvae of hylesia metabus crammer (lepidoptera: saturniidae).hylesia metabus larvae are susceptible to several pathogens indigenous to the area in which they are found. some larvae show symptoms characteristic of bacterial infection; they become flaccid and lethargic, and show a marked loss of appetite. we isolated and identified 29 bacterial strains from live, dead and experimentally infected h. metabus larvae, and evaluated their pathogenic activity. the bacteria which caused mortality in the larvae were: pseudomonas aeruginosa (60-93.3%), proteus vulga ...200212234536
[clinical and pathogenetic significance of proteus mirabilis antibodies in uveitis associated with joint lesions in children and adolescents].immunological studies in 95 children with uveitis associated with juvenile chronic arthritis revealed infection with proteus mirabilis in 14.7% cases, mainly in cases with reactive arthritis and juvenile rheumatoid arthritis (jra). a clear-cut relationship between p. mirabilis infection and severity of the disease was detected. in 85% children infected with p. mirabilis uveitis was associated with a rapid decrease of ocular functions and development of complications, one of the most frequent of ...200212226974
extended-spectrum beta-lactamases.extended-spectrum beta-lactamases (esbls) were discovered in europe in the early 1980s after widespread use of broad-spectrum antibiotics. they are largely derivatives of 3 progenitor beta-lactamases that confer resistance to ampicillin in gram-negative bacteria and are now carried on plasmids. substitutions at the active enzyme site of these progenitor enzymes at single or multiple amino sites have resulted in altered substrate affinities for esbls. depending on the location of the substitution ...200212226798
[antimicrobial, echinococcidial and immunostimulating properties of the drug cheblin-sk-1].the antimicrobial properties of the drug cheblin-ck-1 (cck-1) were determined in mice intraabdominally inoculated with proteus mirabilis-4691 in a dose of 140-200 million daily cultured microbial bodies. its comparison agent was ampicillin. cck-1 was found to act as an antibiotic similar to ampicillin in its effects. the antimicrobial activity of cck-1 against staphylococcus aureus and escherichia coli isolated from the contents of echinococcal cysts from patients operated on was also establishe ...200212224264
chemical constituents and antimicrobial activity of the essential oil of lantana xenica.the aim of this work was to evaluate the chemical composition of lantana xenica essential oil and its antimicrobial activity. the oil from the aerial parts of lantana xenica mold. (verbenacea) was obtained by steam distillation and analyzed by gas chromatography/mass spectrometry. the major constituent of the oil was (e)-caryophyllene (35.2 %), with minor amounts of gamma-cadinene (13.3 %), alpha-pinene (9.3 %), ocimene (9.2 %) and germacrene d (6.6 %). the antimicrobial assays showed that the e ...200212221608
development of an elisa using a recombinant 41 kda partial protein (p45n') for the detection of riemerella anatipestifer infections in ducks.riemerella anatipestifer, a gram-negative bacillus, is the causative agent of duck septicemia, a disease which could incur much economic loss in the duck industry. an indirect enzyme-linked immunosorbent assay (elisa) has been developed to facilitate early detection of r. anatipestifer infection in ducks. the antigen used was a recombinant 41 kda n-terminal fragment (rp45n') of a newly characterized r. anatipestifer potential surface protein, p45, which was expressed in escherichia coli as an n- ...200212220809
first report of a parasitic copepod (pennella balaenopterae) infestation in a pinniped.an infestation by the parasitic copepod pennella balaenopterae was found in a stranded, 8-mo-old, female northern elephant seal (mirounga angustirostris). diagnosis was based on the finding of the cephalothoraxes of 14 adult female copepods from three subcutaneous sites. bacteria cultured from lesion exudate included arcanobacterium phocae, escherichia coli, edwardsiella tarda, an enterococcus sp., and proteus mirabilis. the lesions were drained and irrigated with chlorhexidine, and the seal was ...200212216795
pathology of a mouse model of x-linked chronic granulomatous disease.a colony of knockout mice (gene designation cybb tm1) has been maintained at this institution for 5 years. these mice are lacking the b subunit of nadph oxidase and are susceptible to experimental infection with aspergillus fumigatus. the purpose of this study was to document the spontaneous diseases present in these mice which are a murine model of x-linked chronic granulomatous disease and to compare these lesions to those of chronic granulomatous disease in humans. lesions were documented in ...200212213046
a case of purple urine bag syndrome (pubs). 200212212364
intravitreal penetration of cefepime after systemic administration to humans.to investigate the penetration of cefepime (a fourth-generation cephalosporin) into the vitreous after single-dose intravenous administration to human subjects.200212207129
proteus syndrome and sudden cardiac arrest; beware of pulmonary embolism! 200212204468
[evaluation of the rapid lumi 'eiken' using chemiluminescent assay for rapid antimicrobial susceptibility testing].one hundred ninety eight clinical isolates, including enterobacteriaceae (70 strains), pseudomonas aeruginosa (20 strains), acinetobacter baumannii (10 strains), staphylococci (50 strains), enterococci (20 strains), streptococcus pneumoniae (15 strains) and haemophilus influenzae (13 strains) were tested for their antimicrobial susceptibilities by using the rapid lumi 'eiken' (rl). as a reference method, broth microdilution method according to the national committee for clinical laboratory stand ...200212199112
characterization of flagella produced by clinical strains of stenotrophomonas maltophilia.stenotrophomonas maltophilia is an emerging nosocomial pathogen associated with opportunistic infections in patients with cystic fibrosis, cancer, and hiv. adherence of this organism to abiotic surfaces such as medical implants and catheters represents a major risk for hospitalized patients. the adhesive surface factors involved in adherence of these bacteria are largely unknown, and their flagella have not yet been characterized biochemically and antigenically. we purified and characterized the ...200212194767
[antibiotic properties of the pseudoalteromonas genus bacteria isolated from the black sea water and molluscs].antagonistic properties of 41 strains of alteromonas-like bacteria isolated from the black sea water and molluscs have been studied. being grown on the rich medium "b" for marine bacteria, 21% of strains have shown high antagonistic activity against phytopathogenic fungi; 6% of strains inhibited the growth of bacillus subtilis, proteus vulgaris and candida albicans. spectrum of antagonistic activity was essentially changed on synthetic "bm" medium with acetate, glutamate, alpha-alanine as a sing ...200212190034
[experimental study on bacteriostasis of chitosan and sodium hyaluronate].to compare the effect and coverage of bacteriostasis of chitosan and sodium hyaluronate.200212181792
vaginal microbiota in healthy pregnant women and prenatal screening of group b streptococci (gbs).the microbiota of the lower female genital tract was evaluated from vaginal swabs obtained from 623 healthy pregnant women at gestation periods of 35-40 weeks. isolated and identified microorganisms were expressed as percentages of total samples. as expected, lactobacilli made up the dominant vaginal microbiota (70%). enterobacteriaceae, mainly escherichia coli, klebsiella spp. and proteus, were present in 38% of the samples, which might reflect the possible contamination of vaginal tract with r ...200212180785
[effectiveness of imipenem/cilastatin (tienam, msd) in treating complicated infections in urology].complicated urinary infections tend to eventuate in severe pyoseptic complications--bacteriuria, sepsis. the search for methods of fighting agents of urinary infections goes in the direction of perfection of already existing methods and in the direction of design of novel antibacterial drugs. in the middle 1980s the first carbapenem drug-imipenem--was proposed for urological clinical practice. mechanism of its action as that of the other beta-lactam antibiotics consists in impairment of synthesi ...200212180053
primary cutaneous alveolar rhabdomyosarcoma of the perineum.primary alveolar rhabdomyosarcoma (rms) involving perineal skin is extremely rare, particularly in the infant age group. we report a case of an alveolar rms in a newborn with abnormal symmetrical perineal overgrowth, causing ambiguous morphologic structure of the genitalia. clinical and imaging studies were suggestive of proteus syndrome with lymphatic malformation. histologic examination of the mass showed cutaneous alveolar rms with areas of embryonal and pleomorphic rms features. multiple sup ...200212171502
[bacteriologic examination of gallbladder contents].acute calculous (obstructive) cholecystitis develops as a consequence of cystic obstruction and obstruction of bile flow into choledochus. most often it is a result of impacted gallstones in hartman's pouch or the cystic duct. their direct pressure on gallbladder mucosa causes ischemia, necrosis and ulceration with consequential wall edema and obstructed venous flow. this mechanism is further increasing and spreading the inflammatory process. ulcerations may be that extensive, that mucosa is hig ...200212170867
assessment of peo/ptmo multiblock copolymer/segmented polyurethane blends as coating materials for urinary catheters: in vitro bacterial adhesion and encrustation behavior.the effective long-term use of indwelling urinary catheters has often been hindered by catheter-associated infection and encrustation. in this study, the suitability of poly(ethylene oxide) (peo)-based multiblock copolymer/segmented polyurethane (spu) blends as coating materials for the commercial urinary catheters was assessed by measuring swellability, bacterial adhesion, and encrustation behavior. when exposed to pbs (ph 7.4), the blends absorbed a significant amount of water, which was propo ...200212162332
epidemiology and prognosis of bacteremia: a 10-y study in a community hospital.in order to determine the epidemiology and factors influencing the outcome of adult bacteremia in a community hospital, episodes of significant bacteremia were recorded prospectively over a 10-y period (1989-98). the following variables were included: age, sex, etiology, acquisition and source of the bacteremia, risk factors, clinical manifestations, empirical antibiotic treatment and outcome. a total of 798 episodes of bacteremia were recorded (436 in males) and 185 (24%) were hospital-acquired ...200212160171
evaluation of a new etest for detecting metallo-beta-lactamases in routine clinical testing.several etest (ab biodisk, solna, sweden) gradient formats were developed for detection of metallo-beta-lactamases based on the reduction of imipenem (ip) or ceftazidime (tz) mics in the presence of edta or 2-mercaptopropionic acid (mpa). the etest metallo-beta-lactamase (etest mbl) strips consisted of a double-sided seven-dilution range of ip or tz (4 to 256 microg/ml) and ip or tz (1 to 64 microg/ml) overlaid with a constant concentration of edta or mpa. the prototype strips were evaluated on ...200212149325
interaction of the atypical prokaryotic transcription activator flhd2c2 with early promoters of the flagellar gene hierarchy.the transcriptional activator flhd2c2 is the master regulator of bacterial flagellum biogenesis and swarming migration, activating the "early" class ii promoters of the large flagellar gene hierarchy. using primer extensions, band-shift assays, and enzymatic and chemical footprinting, we describe the binding of the flhd2c2 heterotetramer to the promoter regions of four class ii flagella operons, fliaz, flhba and the divergent flgamn and flgbcd(efghij). each of the promoter regions was bound by a ...200212144778
community-acquired bacterial meningitis in adults: the epidemiology, timing of appropriate antimicrobial therapy, and prognostic factors.between january 1986 and december 1999, 109 adult patients with culture-proven community-acquired bacterial meningitis were identified at kaohsiung chang gung memorial hospital. to compare changes over time, the appearance of disease among our patients was divided into two equal time periods: an earlier time period (1986-1992) and a later time period (1993-1999). in this study, there was a decreasing proportion of community-acquired bacterial meningitis compared with nosocomial bacterial meningi ...200212140104
identification of mrpi as the sole recombinase that regulates the phase variation of mr/p fimbria, a bladder colonization factor of uropathogenic proteus mirabilis.proteus mirabilis is a common cause of urinary tract infection (uti) in individuals with structural abnormalities or long-term catheterization. the expression of mannose-resistant/proteus-like (mr/p) fimbria is phase variable because of the inversion of a 251 bp dna fragment that carries the promoter for the mrp operon. previous studies have shown that mrpi, which is transcribed divergently from the mrp operon, encodes a recombinase capable of switching the orientation of this invertible element ...200212139630
green fluorescent protein urea sensors. uropathogenic proteus mirabilis. 200212136763
strategies for the control of catheter encrustation.two general strategies have been adopted to develop catheter materials that resist encrustaion by bacterial biofilms: (a) the incorporation of antimicrobial agents into the polymers and (b) the production of materials with surface properties which prevent the adherence of bacterial cells. our experience to develop non-adherent surfaces which abstracts design from nature is reported. compounds based on 2-methacryloloxyethylphosphorylcholine co-polymerised with long-chain alkyl methacrylates have ...200212135840
aetiology of chronic prostatitis.a total of 388 patients with symptoms of chronic prostatitis and inflammatory findings in expressed prostatic secretion (eps) or in a urine sample collected immediately after prostate massage, were examined over a 2 year period at the outpatient department for urogenital infections, university hospital for infectious diseases 'dr fran mihaljević', zagreb, croatia. the infective aetiology was determined in 276 (71.13%) patients. chlamydia trachomatis was the causative pathogen in 109 patients, tr ...200212135835
vaccines for proteus mirabilis in urinary tract infection.proteus mirabilis is a documented cause of urinary tract infection (uti) in the complicated urinary tract. urease-mediated urea hydrolysis is responsible for both virulence of the organism and the ability to cause urolithiasis. a urease-negative mutant of p. mirabilis is unable to initiate stone formation and colonizes the kidney at a significantly lower rate. the considerable pathology caused by p. mirabilis warrants the development of a vaccine. we have initiated the advancement of vaccine stu ...200212135833
reactivity to bacterial, fungal, and parasite antigens in patients with lymphedema and elephantiasis.both secondary infections and antifilarial immunity are thought to play roles in the development and progression of lymphedema. to investigate this issue, immune responses to a panel of bacterial, fungal, and parasite antigens were examined for women with lymphedema and elephantiasis (n = 28) and for women with no clinical evidence of lymphatic dysfunction who were either microfilaremic (mf+, n = 23) or microfilaria- and filarial antigen-negative (ag-, n = 24). the prevalence and intensity of de ...200212135288
lomefloxacin versus ciprofloxacin in the treatment of chronic bacterial prostatitis.a total of 182 patients with chronic bacterial prostatitis (cbp) were recruited into this multicentre prospective, randomized clinical study. of these, 93 were treated orally with lomefloxacin (lomx) 400 mg once daily and 89 with ciprofloxacin (cipx) 500 mg twice daily for 4 weeks. at 5-9 days 90/90 vs 86/86, at 4-6 weeks 82/83 vs 82/82, at 3 months 80/79 vs 78/75, and at 6 months 78/75 vs 75/72 patients aging from 18 to 70 years were evaluable bacteriologically/clinically according to a modifie ...200212127707
dna gyrase and topoisomerase iv mutations associated with fluoroquinolone resistance in proteus mirabilis.mutations associated with fluoroquinolone resistance in clinical isolates of proteus mirabilis were determined by genetic analysis of the quinolone resistance-determining region (qrdr) of gyra, gyrb, parc, and pare. this study included the p. mirabilis type strain atcc 29906 and 29 clinical isolates with reduced susceptibility (mic, 0.5 to 2 microg/ml) or resistance (mic, > or =4 microg/ml) to ciprofloxacin. susceptibility profiles for ciprofloxacin, clinafloxacin, gatifloxacin, gemifloxacin, le ...200212121936
resistant pathogens in urinary tract infections.antimicrobial susceptibility of bacteria causing urinary tract infection (uti) has evolved over several decades as antimicrobial exposure has repeatedly been followed by emergence of resistance. older populations in the community, long-term care facilities, or acute care facilities have an increased prevalence of resistant bacteria isolated from uti. resistant isolates are more frequent in long-term care populations than the community. resistant isolates include common uropathogens, such as esch ...200212121518
antibiotic resistance of bacteria associated with community-acquired urinary tract infections in the southern area of the gaza strip.the aim of this study was to identify the microorganisms that cause "community-acquired" urinary tract infections among adults and to investigate their resistance to fourteen selected antimicrobial agents. the uropathogens identified in 121 positive midstream urine cultures from the 270 subjects included in this study were escherichia coli (57.9%), proteus species (9.9%), enterobacter species (7.4%), klebsiella species (6.6%), pseudomonas species (5.8%), staphylococcus saprophyticus (5.0%), ente ...200212120880
emerging antimicrobial resistances among proteus mirabilis in europe: report from the mystic program (1997-2001). meropenem yearly susceptibility test information collection.resistance patterns that are currently problematic in europe can vary greatly within the same species over time, among various patient populations and among geographic regions on the same continent. the results from the meropenem yearly susceptibility test information collection (mystic) program, which monitors carbapenem resistance rates in institutions using meropenem, were used to determine resistance differences among proteus mirabilis. mic results from 688 p. mirabilis strains were classifi ...200212120879
role of pten, a lipid phosphatase upstream effector of protein kinase b, in epithelial thyroid carcinogenesis.both benign and malignant thyroid disease are well-established components of cowden syndrome (cs), an autosomal dominant disorder characterized by multiple hamartomas and breast cancer that may be considered a phakomatosis. the susceptibility gene for cs is pten, a tumor suppressor gene on 10q23.3 that encodes a lipid phosphatase that lies upstream of protein kinase b (akt). interestingly, carney complex is also a phakomatosis where multiple endocrine neoplasias are prominent and thyroid cancer ...200212119278
the etiology of urinary tract infection: traditional and emerging pathogens.the microbial etiology of urinary infections has been regarded as well established and reasonably consistent. escherichia coli remains the predominant uropathogen (80%) isolated in acute community-acquired uncomplicated infections, followed by staphylococcus saprophyticus (10% to 15%). klebsiella, enterobacter, and proteus species, and enterococci infrequently cause uncomplicated cystitis and pyelonephritis. the pathogens traditionally associated with uti are changing many of their features, par ...200212113867
epidemiology and genetics of rheumatoid arthritis.the prevalence of rheumatoid arthritis (ra) is relatively constant in many populations, at 0.5-1.0%. however, a high prevalence of ra has been reported in the pima indians (5.3%) and in the chippewa indians (6.8%). in contrast, low occurrences have been reported in populations from china and japan. these data support a genetic role in disease risk. studies have so far shown that the familial recurrence risk in ra is small compared with other autoimmune diseases. the main genetic risk factor of r ...200212110146
morphine, the proteus of organic molecules.this feature article encapsulates the senior author's longstanding interests in opiate chemistry and attempts to place it within an historical context and against the backdrop of related work by others who have viewed morphine as one of the pinnacles of natural product synthesis. biomimetic and 'bioanalogous' routes to the morphine skeleton are discussed followed by approaches based on the elaboration of phenanthrene platforms. the latter include an asymmetric synthesis of ent-morphine developed ...200212109065
swarming-coupled expression of the proteus mirabilis hpmba haemolysin operon.the hpma haemolysin toxin of proteus mirabilis is encoded by the hpmba locus and its production is upregulated co-ordinately with the synthesis and assembly of flagella during differentiation into hyperflagellated swarm cells. primer extension identified a sigma(70) promoter upstream of hpmb that was upregulated during swarming. northern blotting indicated that this promoter region was also required for concomitant transcription of the immediately distal hpma gene, and that the unstable hpmba tr ...200212101306
a comparison of the performance of cystine lactose electrolyte deficient (cled) agar with oxoid chromogenic urinary tract infection (cuti) medium for the isolation and presumptive identification of organisms from urine.as part of the uk antimicrobial resistance strategy and action plan, the public health laboratory service (phls) is required to collect antibiotic susceptibility data so that resistance trends and patterns can be monitored. most laboratories report urine gram negative isolates, as "coliforms" according to morphological appearance, but without an acceptable identification system the antimicrobial surveillance data will be meaningless. commercially available identification systems tend to be expen ...200212101200
survival of enterobacteria in liquid cultures during microwave radiation and conventional heating.bacteria in food have been reported to survive in larger numbers after processing by microwave radiation than after conventional processing. the bactericidal effect of a domestic microwave oven (sharp r-7280) on certain pathogenic enterobacteria species was investigated in vitro, in comparison with conventional heating (boiling). the death rates of different nosocomial strains of escherichia coli, salmonella sofia, salmonella enteritidis, proteus mirabilis and pseudomonas aeruginosa were tested. ...199512099299
[species composition and microbial contamination of atmospheric humidity condensate and potable water from the mir water supply systems during mir main missions 4 through 27].analyzed were quantitative and qualitative characteristics, and processes of formation and transformation of microflora in the atmospheric humidity condensate (ahc) and potable water (pw) regenerated from the condensate by the long-operating system (srv-k) aboard space station mir. the paper presents data on species composition of microflora of ahc, and pw regenerated by srv-k in the period from 1989 through to 1999. the following microbial species were identified in ahc: alcaligenes (14.8%), ba ...200212098948
multidrug resistant neonatal sepsis in peshawar, pakistan.to investigate the spectrum of organisms causing neonatal sepsis in peshawar, pakistan and to assess their sensitivity to various groups of drugs.200212091293
emerging fluoroquinolone-resistance for common clinically important gram-negative bacteria in taiwan.the in vitro antimicrobial activity of the three most commonly used fluoroquinolones (norfloxacin, ofloxacin and ciprofloxacin) was measured for 2235 isolates of seven common pathogenic gram-negative bacteria. collected from a major teaching hospital in taiwan during the years 1985-86, 1989-90, and 1996-97, the samples were evaluated using the agar dilution method. the overall susceptibility to fluoroquinolones has decreased rapidly after the wide use of these antimicrobial agents. isolates of e ...200212088622
bacterial population of a two-phase anaerobic digestion process treating effluent of cassava starch factory.different groups of microorganisms in a two-phase anaerobic system were enumerated to evaluate the prevalence of specific groups and species. total and fecal coliforms showed similar values both in acidogenic and methanogenic reactors. the fecal streptococci were 4-fold higher in the acidogenic reactor, when compared with those of the methanogenic reactor. as expected, no methane forming or sulfate reducing bacteria were found in the acidogenic reactor. the populations of methanogenic bacteria w ...200212088382
the molecular mechanism of translocation through the nuclear pore complex is highly conserved.in this report we investigated the activity of vertebrate nuclear transport factors in a primitive organism, amoeba proteus, to better understand evolutionary changes in the transport mechanisms of organisms expected to have different requirements for nucleocytoplasmic exchange. it was initially determined that fxfg-containing nucleoporins and ran, both of which are essential for nuclear import in vertebrates, as well as yeast, are also present and functional in amoebae. this suggests that there ...200212082159
synthesis and pharmacological activities of 1,8-naphthyridine derivatives.in the present study, a series of 2-substituted-4-methyl-7-amino/4,7-dimethyl-1,8-naphthyridines were synthesized and characterized by ir, 1h-nmr and elemental analysis. the compounds were investigated for anticonvulsant (125, 250 mg/kg), cardiac and antimicrobial activities. the compounds were screened for antibacterial activity against gram (+) bacteria (staphylococcus epidermidis, bacillus subtilis, enterococcusfaecalis and micrococcus luteus) and gram (-) bacteria (proteus vulgaris, pseudomo ...200212081151
[experimental study on detection of schistosoma japonicum 5d gene by using pcr].to search for an effective method to diagnose the schistosomiasis and evaluate the effect of treatment.199812078210
[use of sparfloxacin (sparflo) in treating complicated urologic infections].the authors review the effectiveness of a novel fluoroquinolone drug sparfloxacine (sparflo, dr. reddy's laboratories) in the treatment of patients with complicated forms of pyelonephritis and prostatitis. both gramnegative and grampositive agents causing complicated urologic infections were highly sensitive to sparfloxacine: enterobacter spp.--40.8%, pseudomonas aeruginosa--38.5%, proteus spp.--42.6%, e. coli--91.4%, staphylococcus spp.--80.0%, enterococcus faecalis--21.4%. sparfloxacine was us ...200212077817
principles for the surgical management of patients with proteus syndrome and patients with overgrowth not meeting proteus criteria.proteus syndrome is a rare, sporadic disorder consisting of disproportionate overgrowth of multiple tissues, vascular malformations, and connective tissue or epidermal nevi. patients with proteus syndrome present with diverse and variable phenotypes because of the syndrome's mosaic pattern of distribution.200212077761
proteus syndrome of the hand.proteus syndrome exhibits an expanded phenotype. we report a case with restricted macrodactyly and cerebriform hypertrophy of one palm. the differential diagnosis with macrodactyly and other hamartomatous overgrowth syndromes is discussed.200212077537
numbers and types of microorganisms in vacuum-packed cold-smoked freshwater fish at the retail level.fifty-four packages (each one belonging to a different lot) of vacuum-packed cold-smoked salmon (30) and trout (24) produced by six spanish smokehouses were obtained at retail level after 3 weeks storage at 2+/-1 degrees c. sensorial, chemical, physicochemical and microbiological characteristics were examined. overall, ph, a(w), salt content in water phase, aerobic plate counts at 30 and 25 degrees c. levels of enterobacteriaceae, lactic acid bacteria (lab), fungi and presumptive aeromonads and ...200212076034
comparative evaluation of two commercial chromogenic media for detection and presumptive identification of urinary tract pathogens.the performance of two commercial chromogenic media for the isolation and presumptive identification of urinary tract pathogens, the cps id2 (biomérieux, france) and the chromagar orientation (bbl becton dickinson, usa), was evaluated and compared with that of cystine-lactose-electrolyte-deficient agar and tryptic soy agar with 5% sheep blood. the detection, determination of bacterial counts, and presumptive identification of bacteria causing urinary tract infections were evaluated in 3,000 urin ...200212072939
[yearly changes in antibacterial activities of cefozopran against various clinical isolates between 1996 and 2000--ii. gram-negative bacteria].the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates obtained between 1996 and 2000 were yearly evaluated and compared with those of other cephems, oxacephems, and carbapenems. thirty-two species 2,697 strains of gram-negative bacteria were isolated from the clinical materials annually collected from january to december, and consisted of moraxella subgenus branhamella catarrhalis (n = 125), escherichia coli (n = 250), citrobacter freu ...200212071094
new carbenicillin-hydrolyzing beta-lactamase (carb-7) from vibrio cholerae non-o1, non-o139 strains encoded by the vcr region of the v. cholerae genome.in a previous study, an analysis of 77 ampicillin-nonsusceptible (resistant plus intermediate categories) strains of vibrio cholerae non-o1, non-o139, isolated from aquatic environment and diarrheal stool, showed that all of them produced a beta-lactamase with a pi of 5.4. hybridization or amplification by pcr with a probe for bla(tem) or primers for bla(carb) gene families was negative. in this work, an environmental ampicillin-resistant strain from this sample, me11762, isolated from a waterwa ...200212069969
[a case of stone formation in the mainz pouch using appendix as the efferent limb: a case report].a large calculus (6.0 x 4.5 x 7.0 cm sized) in the continent urinary pouch was detected incidentally by computed tomography in a 7-year-old man with a gall bladder stone. he underwent a total cystectomy and mainz pouch formation using appendix as the efferent limb for bladder cancer 7 years ago. urine culture showed proteus mirabilis and escherichia coli, and urine ph was 8.5. the stone was removed successfully through a pouchotomy and composed mainly of struvite. the stone analysis was map; 88% ...200212056043
a novel ophthalmic presentation of the proteus syndrome.to report a patient with the proteus syndrome who had ocular complications not previously described in the literature.200212045066
[piperacillin/tazobactam--tazocin. a penicillin-based alternative to 3rd generation cephalosporins and carbapenems].the antibiotic piperacillin/tazobactam has recently been licensed for use in denmark. piperacillin/tazobactam combines a well known beta-lactam antibiotitic, piperacillin, and an inhibitor of bacterial beta-lactamase, tazobactam. the combination of piperacillin and tazobactam compared to piperacillin alone has an expanded antimicrobial spectrum, which includes klebsiellae, escherichia coli, and proteus vulgaris resistant to ampicillin, as well as beta-lactamase-producing staphylococcus aureus. a ...200212043402
evaluation of the dipstreak, a new device with an original streaking mechanism for detection, counting, and presumptive identification of urinary tract pathogens.dipstreak is a new urine culture device with two types of agar attached back-to-back on a plastic paddle. it combines dip-slide technology and an original streaking inoculation mechanism, allowing for bacterial counting and colony isolation. the performance of the dipstreak device with two different medium formulations, chromagar and macconkey media in study a and uriselect 3 and macconkey media in study b, was evaluated and compared to that of the reference streak method by using plates of cyst ...200212037082
detection of plasmid-mediated ampc beta-lactamase genes in clinical isolates by using multiplex pcr.therapeutic options for infections caused by gram-negative organisms expressing plasmid-mediated ampc beta-lactamases are limited because these organisms are usually resistant to all the beta-lactam antibiotics, except for cefepime, cefpirome, and the carbapenems. these organisms are a major concern in nosocomial infections and should therefore be monitored in surveillance studies. six families of plasmid-mediated ampc beta-lactamases have been identified, but no phenotypic test can differentiat ...200212037080
bacterial contamination of saline nasal spray/drop solution in patients with respiratory tract infection.this study evaluated the potential for bacterial contamination of saline nasal spray/drop solution after 3 days of clinical use in patients. twenty patients with upper respiratory infection used the saline as a spray, and 20 patients used the solution as drops. bacterial growth was present in 18 (907%) solution containers of the spray group and in 3 (157%) solution containers of the drops group (p <.005). twenty isolates were recovered from the spray group and 3 from the drops group. the predomi ...200212032501
[rational use of medications in the treatment of urinary infections].main: to encourage the rational use of medication in the treatment of uncomplicated acute cystitis (uac). secondary: to find the most common germs in our area and their sensitivity to medication, the most common anti-microbials used and patients age, and to evaluate the savings made through a policy of rational use of medication.design. retrospective, descriptive, observational study.200212031221
complete nucleotide sequence of plasmid rts1: implications for evolution of large plasmid genomes.rts1, a large conjugative plasmid originally isolated from proteus vulgaris, is a prototype for the inct plasmids and exhibits pleiotropic thermosensitive phenotypes. here we report the complete nucleotide sequence of rts1. the genome is 217,182 bp in length and contains 300 potential open reading frames (orfs). among these, the products of 141 orfs, including 9 previously identified genes, displayed significant sequence similarity to known proteins. the set of genes responsible for the conjugat ...200212029035
proteus syndrome.proteus syndrome is a rare congenital hamartomatous entity. we report some new findings in a boy with this syndrome, in addition to the classical clinical and radiological features of subcutaneous masses, partial gigantism of hands and feet, bony abnormalities, and epidermal nevi. we also discuss how to reach a definitive diagnosis by using an established rating scale.200212027091
activity of ocimum sanctum (the traditional indian medicinal plant) against the enteric pathogens.aqueous & alcoholic extracts of o. sanctum were prepared. two concentrations of these extracts (30 mg & 60 mg) were tried against the enteric pathogens & candida albicans by agar diffusion method. wide zones of inhibition were observed at 60 mg concentration of extract. aqeous extract showed wider zone of inhibition when compared to alcoholic extract. aqueous extract showed wider zones of inhibition for klebisella, e. coil, proteus & staphylococcus aureus. alcoholic extract showed wider zone for ...200112026506
[erythropoietin and hip surgery in an anemic jehovah's witness with ankylosing spondylitis, chronic liver disease, and crohn's disease]. 200212025242
in vitro susceptibility of new generation of antibacterial antibiotics against pathogenic bacteria.antibiotic susceptibility of ten bacteria i.e. neisseria catarrhalis, salmonella typhi, s. enteritidis, haemophilus influenzae, bacillus subtilis, pseudomonas fluorescence, pseudomonas aeruginosa, proteus vulgaris, staphylococcus aureus and e. coli to twenty antibiotics i.e. cefpirom (30 mcg), ceftriaxone (30 mcg), erythromycin (15 mcg), doxycycline (30 mcg) lomefloxacin (10 mcg), sisomicin (30 mcg), vancomycin (30 mcg), augmentin (30 mcg), ampicillin (30 mcg), cotrimoxazole (25 mcg), cefotaxime ...199912024980
competitive advantages of caedibacter-infected paramecia.intracellular bacteria of the genus caedibacter limit the reproduction of their host, the freshwater ciliate paramecium. reproduction rates of infected strains of paramecia were significantly lower than those of genetically identical strains that had lost their parasites after treatment with an antibiotic. interference competition occurs when infected paramecia release a toxic form of the parasitic bacterium that kills uninfected paramecia. in mixed cultures of infected and uninfected strains of ...200212022275
effect of tumor necrosis factor alpha, interferon gamma, and interleukin-4 on bacteria-enterocyte interactions.little is known about the mechanisms involved in bacterial translocation from the intestinal lumen to extraintestinal sites. because the cytokine cascade associated with sepsis, inflammation, and trauma has been shown to affect intestinal epithelial permeability, experiments were designed to clarify the effects of selected cytokines on bacterial adherence to and internalization by cultured ht-29 and caco-2 enterocytes.200212020125
chromosome-encoded class d beta-lactamase oxa-23 in proteus mirabilis.ten nonrepetitive proteus mirabilis isolates, which were collected over 4 years (1996 to 1999) at the teaching hospital of clermont-ferrand, france, produced class d carbapenemase oxa-23. mics of imipenem were 0.25 to 0.5 microg/ml for these clinical isolates. molecular typing revealed that the 10 p. mirabilis isolates originated from the same clonal strain. hybridization of i-ceui-generated chromosome fragments with a bla(oxa-23) probe showed that the gene was chromosome encoded in the p. mirab ...200212019126
[profile and sensitivity to antibiotics of 2063 uropathogenic bacteria isolated in the center of tunisia].current bacteriological data are very useful when making therapeutic decisions in cases of non complicated urinary tract infection. in this article, we present the data gathered by a university hospital laboratory in mid-tunisia as well as the results of 17,829 urinary cytobacteriological examinations conducted in a multidisciplinary hospital during the year 2000. urine was sowed on usual agar. all bacterium cultivating at least 10(5) bacteria reported to ml and at 37 degrees c in a normal atmos ...200212012968
ceftriaxone activity against gram-positive and gram-negative pathogens isolated in us clinical microbiology laboratories from 1996 to 2000: results from the surveillance network (tsn) database-usa.ceftriaxone was introduced into clinical practice in the usa in 1985 and was the first extended-spectrum (third-generation) cephalosporin approved for once-daily treatment of patients with gram-positive or gram-negative infections. review of ceftriaxone activity is important given its continued use since the mid-1980s and reports of emerging resistance among all antimicrobial agent classes. we reviewed the activity of ceftriaxone and relevant comparative agents against five gram-positive and 11 ...200212007850
diversity of ctx-m beta-lactamases and their promoter regions from enterobacteriaceae isolated in three parisian hospitals.nine clinical isolates of enterobacteriaceae (six escherichia coli and three proteus mirabilis) isolated in three parisian hospitals between 1989 and 2000 showed a particular extended-spectrum cephalosporin-resistance profile characterized by resistance to cefotaxime and aztreonam but not to ceftazidime. ctx-m-1, ctx-m-2, ctx-m-9, ctx-m-14 and two novel plasmid-mediated ctx-m beta-lactamases (ctx-m-20, and ctx-m-21) were identified by polymerase chain reaction and isoelectric focusing (pi>8) and ...200212007800
evaluation of n-acetylchitooligosaccharides as the main carbon sources for the growth of intestinal bacteria.n-acetylchitooligosaccharides ((glcnac)(n)) with different degrees of polymerization (n=1-6) were prepared as the main carbon sources in media for evaluating the growth of nine intestinal bacteria. a chitohydrolysate was prepared by hydrolyzing shrimp-shell chitin using hcl. after purification, the purity of each (glcnac)(1-6) was >86%. the growth of intestinal bacteria was carried out in a basal medium (bm) containing 0.2% (w/v) of each sugar or glucose as the main carbon source and was evaluat ...200212007653
susceptibility of antibiotic-resistant gram-negative bacteria to biocides: a perspective from the study of catheter biofilms.bacteria resistant to both the agents deployed to prevent infections and those used to treat infections would be formidable nosocomial pathogens. the aim of this paper is to review the evidence that gram-negative bacteria resistant to antibiotics and biocides have emerged and been responsible for catheter-associated urinary tract infection. a study of patients undergoing intermittent bladder catheterization revealed that the frequent application of the antiseptic chlorhexidine to the perineal sk ...200212000625
the structure of the carbohydrate backbone of the rough type lipopolysaccharides from proteus penneri strains 12, 13, 37 and 44.the following structure of the lipid a-core backbone of the rough type lipopolysaccharides (lps) from proteus penneri strains 12, 13, 37, and 44 was determined using nmr and mass spectroscopy and chemical analysis of the oligosaccharides obtained by mild-acid hydrolysis, alkaline o,n-deacylation, o-deacylation with hydrazine, and deamination of the lpss:where k=h, r=petn, r(1)=alpha-hep-(1-->2)-alpha-ddhep, and r(2)=alpha-galn (strains 12 and 13) or beta-glcnac-(1-->4)-alpha-glcn (strains 37 and ...200211996837
trends in fluoroquinolone (ciprofloxacin) resistance in enterobacteriaceae from bacteremias, england and wales, 1990-1999.the public health laboratory service receives antibiotic susceptibility data for bacteria from bloodstream infections from most hospitals in england and wales. these data were used to ascertain resistance trends to ciprofloxacin from 1990 through 1999 for the most prevalent gram-negative agents: escherichia coli, klebsiella spp., enterobacter spp., and proteus mirabilis. significant increases in resistance were observed for all four species groups. for e. coli, ciprofloxacin resistance rose from ...200211996681
urinary pathogens' resistance to common antibiotics: a retrospective analysis.the susceptibility of urinary pathogens to common antibiotics was investigated and the results analysed retrospectively using the whonet computer program. of 1776 urine samples (44 catheterized) processed, 510 (28.7%) urinary pathogens were isolated. of these 510 positive cultures, 455 (89.2%) were gram-negative bacilli, 45 (8.8%) candida species and 10 (1.9%) gram-positive cocci. of the 44 catheterized samples, 32 (72.7%) yielded significant bacteriuria and these were mainly gram-negative bacte ...200211991018
dual identities. 200211986637
Displaying items 4001 - 4100 of 15180