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[enteropathogenicity of proteus mirabilis bacteria and pathogenesis of experimental intestinal infections caused by them]. 19853917194
aztreonam therapy for gram-negative pneumonia.nineteen patients with community- or hospital-acquired gram-negative pneumonia were treated with aztreonam. other gram-negative antibiotics were withheld. thirteen patients (68 percent) had clinical cures and 15 (79 percent) had microbiologic cures with aztreonam. no adverse reactions or drug toxicity occurred in this population.19853918444
[obtaining antisera to pseudomonas aeruginosa and proteus antigens for performing immunoenzyme analyses in clinical practice].the data presented in this work indicate that specific antisera to p. aeruginosa and proteus antigens can be produced by using extracts from these microorganisms, destroyed by ultrasonic treatment or by multiple freezing and thawing, for the immunization of rabbits. blood serum samples from patients with purulent septic complications were studied for the presence of p. aeruginosa and proteus antigens in elisa with the use of peroxidase-labeled antibodies from antisera to p. aeruginosa and proteu ...19853920845
xanthine calculi in the patient with the lesch-nyhan syndrome associated with urinary tract infection.a japanese boy with lesch-nyhan syndrome who passed xanthine calculi is reported. after pyelolithotomy for a left renal stone, made up of ammonium urate, associated with urinary tract infection, a high dose of allopurinol was given because of the persistence of pyuria. in the present case, the administration of a high dose of allopurinol, given for the prevention of ammonium urate stone formation in infected urine, induced xanthine calculi formation and we had difficulty in the management of thi ...19853925607
amyloidosis as a complication of cystic fibrosis.amyloidosis appears to be a rare complication of cystic fibrosis. we discuss three patients with amyloidosis complicating cystic fibrosis to add to the six patients previously recorded. the presenting problem was proteinuria in five patients, thyromegaly in three patients, and hepatosplenomegaly in one patient. the progression of proteinuria to nephrotic syndrome and edema occurred in eight of nine patients and portended a very poor prognosis. the kidneys, adrenal glands, spleen, thyroid gland, ...19853925758
1-ethyl-6-fluoro-1,4-dihydro-4-oxo-7-(1h-pyrrol-1-yl)-quinoline-3-carb oxylic acid, a new fluorinated compounds of oxacin family with high broad-spectrum antibacterial activities.the synthesis of 1-ethyl-6-fluoro-1,4-dihydro-4-oxo-7-(1h-pyrrol-1-yl)quinoline-3-carboxy lic acid, a new fluorinated high broad-spectrum antibacterial agent related to nalidixic acid is described. the title compound has been prepared by the reaction of 4-fluoro-3-(1h-pyrrol-1-yl)aniline with diethyl ethoxymethylenemalonate, cyclization of the malonate obtained to the quinolinecarboxylate ester, ethylation of the ester, followed by hydrolysis with aqueous sodium hydroxide. the new derivative pro ...19853926533
the diagnostic utility of the febrile agglutinin tests.the purpose of our study was to analyze the use of febrile agglutinin (fa) serologic tests in the diagnosis of salmonella (typhi and paratyphi), rickettsia, brucella, and francisella infections. data were collected in a retrospective chart study of 202 titer requests covering a 42-month period at our teaching hospital. we found that no diagnoses were made by utilizing complete six-antigen fa panels. three cases of infection were diagnosed serologically, all three with more discriminately selecte ...19853927019
[use of opportunistic gram-negative microorganisms for stimulating antitumor resistance]. 19853927057
in vitro pyocin activity of pseudomonas aeruginosa strains pretreated with antibiotics.fifty-six selected strains of pseudomonas aeruginosa belonging to 8 different pyocin types (h, i, 15, 6, pti-1, pti-2, pti-3, pti-4) were treated with subinhibitory concentrations (mic/2) of either gentamicin or carbenicillin. both treatments induced changes in pyocin patterns for all types but at different levels. the percentage of strains that retained their pyocin pattern were more or less equal in both treatments. in treated and untreated producers, the growth inhibition ability for 5 differ ...19853928466
the in vitro activity and beta-lactamase stability of cefpirome (hr 810), a pyridine cephalosporin agent active against staphylococci, enterobacteriaceae and pseudomonas aeruginosa.the in vitro activity of cefpirome, a new cyclopyridinium cephalosporin, was evaluated against 947 aerobic and anaerobic bacteria. cefpirome inhibited 90% of escherichia coli, klebsiella spp., citrobacter diversus, morganella morganii, proteus vulgaris, proteus mirabilis, aeromonas spp., salmonella spp., shigella spp. and haemophilus and neisseria species at less than or equal to 0.4 mg/l. it had activity comparable to that of cefotaxime, ceftizoxime, ceftazidime, aztreonam, and moxalactam again ...19853928497
[activity of the amoxicillin-clavulanic acid (augmentin) combination on strains of hospital isolates].all strains of staphylococcus aureus and gram negative bacilli (enterobacteriaceae, pseudomonas aeruginosa, acinetobacter) isolated from 01.01 to 03.31.83 were studied using agar diffusion with augmentin-impregnated discs (amoxicillin 20 micrograms + clavulanic acid 10 micrograms). augmentin is active against penicillinase-producing staphylococci susceptible to methicillin, whereas methicillin-resistant strains are also resistant to augmentin. according to the susceptibility of strains to amoxic ...19853929215
antibiotic-resistant flora in nursing home patients admitted to the hospital.to study carriage of multiply resistant gram-negative bacilli, 50 patients admitted to the hospital from nursing homes (nhs) and 50 control admissions not from nhs were matched for age and recent antibiotic use. their antibiotic resistance patterns were similar: 20 nh patients and 14 controls had resistant strains. however, significantly more patients (64%) from nhs with large numbers of "skilled beds" had resistant bacteria than did patients from small nhs (21%) or controls (28%). also, more pa ...19853929707
[laboratory and clinical studies on cefminox].laboratory and clinical studies were performed on cefminox (cmnx, mt-141), a new cephamycin antibiotic, and results were as follows. antimicrobial activities. mics of cmnx against various clinical isolates were determined with the inoculum size of 10(6) cells/ml. percentages of strains susceptible to 12.5 micrograms/ml or less were 4% for s. aureus, 0% for e. faecalis, 100% for e. coli, 81% for k. pneumoniae, 3% for enterobacter sp., 18% for s. marcescens, 90% for p. mirabilis, 88% for indole-po ...19853930782
[basic and clinical studies on cefminox in the field of obstetrics and gynecology].fundamental and clinical studies on cefminox (cmnx, mt-141), a new cephamycin antibiotic, were carried out and the following results were obtained. when cmnx was administered at a dose of 1 g by intravenous drip infusion taking 1 hour, the serum concentrations as high as 20 micrograms/ml and 21 micrograms/ml were detected even at 4 hours after administration for the uterine artery and elbow vein, respectively. from these results, cmnx was supposed to maintain in vivo concentrations high enough t ...19853930801
[distribution and changes in the susceptibility of bacteria isolated from clinical samples. iii].this report presents data concerning in vitro activity of antimicrobial agents against citrobacter freundii, enterobacter spp., serratia marcescens and proteus vulgaris isolated from patients with complicated urinary tract infections and against pseudomonas aeruginosa isolated from surgical wounds with postoperative infection and exudate from superficial abscesses. there was a marked increase of resistant strains of c. freundii, enterobacter spp. and s. marcescens to penicillins, ceps or gm. the ...19853930806
bilateral and symmetrical osteomyelitis due to gram-negative bacilli. a report on two cases.gram-negative bacillary osteomyelitis secondary to sepsis is a rare condition. it most frequently attacks the vertebrae, occasionally in the long bones, and can on occasion be multiple. we present 2 patients with severe sepsis of urinary origin who, after a latent period, developed bilateral and symmetrical osteomyelitis due to the same microorganism that caused the sepsis. we discuss this peculiar presentation, its possible pathogenetic mechanism and the need to consider osteomyelitis in the pr ...19853931213
growth kinetics of mixed culture in salmonella enrichment media.a technique for investigating the kinetics of salmonella enrichment is reported. its use with four enrichment media (rappaport's medium, muller-kauffmann tetrathionate broth (mkt) tetrathionate broth and selenite f) is described and the effect of elevated temperature on the growth kinetics shown. rappaport's medium at 37 degrees c and mkt at either 37 degrees c or 42 degrees c were far superior to selenite f and tetrathionate broth in their selective properties and, with the exception of rappapo ...19853932292
further characterization of bacterial adherence to urinary bladder mucosa: comparison with adherence to anion exchange resin.bacterial adherence to anion exchange resin has recently been reported to provide a useful, rapid, in vitro screening assay for identifying putative antiadherence agents. the studies presented in this report provide additional evidence that adherence to anion exchange resin is similar to urinary bladder mucosa adherence. results are as follows. 1) heparin inhibits the adherence of escherichia coli, klebsiella ozaenae, proteus mirabilis, streptococcus fecalis and pseudomonas aeruginosa to both th ...19853932676
[chronic urinary tract infection of the patient with neurogenic bladder--with special reference to bacteriological study in relation to the kinds of urological management]. 19853932736
[comparative evaluation of skin tests in surgical patients].a comparative estimation of the prognostic value of skin tests was made with tuberculin, coli-bacillus antigens, candida, blue pus bacillus and more complete set of microbic allergens (hemolytic staphylococcus, white streptococcus, blue pus bacillus, coli-bacillus, group pneumococcus, candida, proteus mirabilis). high prognostic information value was noted by results of skin tests in patients examined with the help of a set consisting of 7 antigens.19853933160
detection of beta-lactamase activity with nitrocefin of multiple strains of various microbial genera.the production or presence of beta-lactamase(s) was studied by the rapid method utilizing the chromogenic cephalosporin compound nitrocefin in cultures of multiple strains belonging to the same genus as well as groups of microorganisms. the genera were: staphylococcus spp., streptococcus faecalis, escherichia coli, klebsiella pneumoniae, proteus mirabilis, indole-positive proteus spp., enterobacter spp., serratia marcescens, rare enterobacteriaceae, pseudomonas aeruginosa, haemophilus influenzae ...19853933272
formadicins, new monocyclic beta-lactam antibiotics of bacterial origin. i. taxonomy, fermentation and biological activities.a gram-negative bacterium produces new monocyclic beta-lactam antibiotics with a formylamino substituent, named formadicins a, b, c and d. the producing bacterium was taxonomically characterized and designated as flexibacter alginoliquefaciens sp. nov. yk-49. formadicins have narrow antibacterial spectra. they are highly active against some species of pseudomonas, proteus and alcaligenes. of the four, formadicin c shows the most potent antibacterial activity. several amino acids such as glycine, ...19853934120
infection control during parenteral nutrition therapy.parenteral nutrition therapy can achieve an anabolic state in patients who are unable to maintain normal nitrogen balance; however, it may be associated with infectious complications. infections may be related to contamination of the cannula and the cannula wound, of the infusate, or of other parts of the parenteral nutrition system. a variety of microorganisms has been associated with these infections. the exact mechanisms that initiate cannula-related infection are poorly understood. susceptib ...19853934408
spotted fever group rickettsiosis in japan.the acute febrile disease with characteristic rash encountered in tokushima prefecture was proved to be a spotted fever group rickettsiosis, which showed a significant rise in agglutinins to both proteus ox2 and ox19 and significantly high levels of cf antibodies to rickettsia akari and rickettsia rickettsii.19853934436
[in vitro activity of norfloxacin against gram-positive and gram-negative organisms from recent clinical isolates].a total of 274 recently isolated gram-positive e gram-negative strains have been tested. the method used for the determination of the minimal inhibitory concentration of the antibiotics was by agar dilution and the minimal bactericidal concentrations were determined by "replica plating" system. all strains demonstrated a good sensitivity, above all, to norfloxacin which inhibited gram-positive bacteria at the concentration of 3.12 micrograms/ml and, concerning gram-negative, at the concentration ...19853935135
trends in bacterial susceptibility to antimicrobial agents.concern over the development of drug resistance by common pathogens has grown over recent years. in order to discover and describe possible trends at this institution, susceptibility data for 18 organisms and 21 antimicrobial agents for the years 1975 through 1982 at the veterans administration medical center in oklahoma city were reviewed. summaries of susceptibility data for 64 major combinations of drugs and organisms are presented. two gram-positive organisms (enterococcus group d and staphy ...19853935395
use of a polyvalent autogenous bacterin for treatment of mixed gram-negative bacterial osteomyelitis in a rhinoceros viper. 19853935615
[comparative studies of antimicrobial agents against causative organisms isolated from urinary tract infections (1983). iii. secular changes in susceptibility].the in vitro antimicrobial activities of antibiotics against causative pathogens isolated from patients with urinary tract infections (uti) in the 8 institutions in japan during the period from 1980 to 1983 were compared. a number of new beta-lactam antibiotics with broad spectrum of activity have become available for clinical use in recent years. some of them, in particular so-called third generation cephems, are reported to be responsible for developing microbial-cross resistance to multiple b ...19853935824
[consumption of aminoglycosides and incidence of resistant gram-negative bacteria in the regional hospital of perugia]. 19853936663
bacteriology and ultrastructure of the bladder in patients with urinary tract infections.tissue obtained by biopsy of the bladder from 33 patients with various urinary tract symptoms was studied by conventional bacteriological techniques and by scanning electron microscopy (sem). all the patients had recurrent urinary tract symptoms which had not completely responded to antibiotic therapy. eleven of the patients had greater than or equal to 10(8) colony-forming units (cfu) of bacteria/1 urine; six had between 10(6) and 10(7) cfu/1, and 16 were abacteruric. bacteria were isolated, ho ...19853936879
[effect of a ticarcillin-clavulanic acid (timentin) combination on bacteria resistant to ticarcillin].sixty-nine ticarcillin-resistant strains (57 gram negative bacilli and 12 s. aureus) were tested: mics and mbcs were determined for clavulanic acid, ticarcillin, and both agents combined using a liquid micromethod. mics were compared to results of disk antibiotic sensitivity tests. while clavulanic acid exhibits little antibacterial activity, its action is synergistic with that of ticarcillin making 100% of staphylococci and 84.2% of gram negative bacilli susceptible. among the gram negative bac ...19853937121
[in vitro effect of ceftriaxone on hospital bacteria. line of regression and critical values].antimicrobial activity of ceftriaxone, a new third generation cephalosporin, against hospital isolates was investigated. 312 strains belonging to the most commonly recovered species were studied using the following methods: agar diffusion (disk antibiotic testing), agar dilution (determination of mic) and dilution in a liquid medium with subculture to agar plates (determination of mbc). except for a few citrobacter strains and rare serratia strains, all enterobacteriaceae tested proved highly su ...19853937122
[changes in the migration and adhesive activity of polymorphonuclear leukocytes as 1 of the characteristics of gram-negative bacteria isolated from patients with suppurative lung destruction].the influence of gram-negative bacteria on the migratory and adhesive activity of polymorphonuclear leukocytes (pmnl) in the peripheral blood of clinically normal donors has been studied by the specially developed method with the use of boyden chambers. pseudomonas and enterobacteria have been found to produce complex and various effects on the above-mentioned properties of pmnl. when incubated in fresh serum, gram-negative bacteria are capable of enhancing the migratory activity of pmnl, this p ...19853937393
bacteriological study of renal calculi.the pre-operative urine, pelvic urine, removed calculi and calculus washings were examined bacteriologically in 24 patients undergoing removal of intrarenal calculi. four of seven patients with struvite calculi had an infected pre-operative midstream urine specimen and six of the seven removed calculi demonstrated significant bacterial growth. proteus mirabilis was the commonest organism isolated. of 17 patients with oxalate calculi only one had an infected pre-operative urine culture, but in fo ...19853937732
resistance to beta-lactamase-stable cephalosporin antibiotics in strains of enterobacteriaceae and pseudomonas aeruginosa isolated from blood cultures.a total of 224 strains of enterobacteriaceae and pseudomonas aeruginosa isolated from blood cultures were screened for resistance to cefuroxime and cefotaxime. seven of 200 strains of enterobacteriaceae were resistant to either cefuroxime or cefotaxime, while five of 24 strains of ps. aeruginosa were resistant to cefotaxime. a further 32 resistant variants were isolated by use of a single step selection procedure, without prior mutagenesis, from strains which were sensitive on initial screening. ...19853937838
ofloxacin: in-vitro activity against clinical isolates.the in-vitro activity of ofloxacin, a new quinolone derivative, was evaluated in 273 bacterial strains isolated from urine. the results showed a remarkable activity against most gram-negative bacteria and, in particular, against enterobacter aerogenes, which is very difficult to eradicate even with third-generation cephalosporins.19853938347
[evaluation of weil-felix test results by comparing the agglutinability of proteus ox2 and ox19 commercial antigens: basis for the presumption of a spotted fever group rickettsiosis]. 19853938464
[the phenomenon of bacterial adhesion in urinary isolates to cells of the urogenital tract after contact with subinhibitory concentrations of netilmicin]. 19853938744
behaviour of cefmenoxime towards beta-lactamases.using several well-characterized beta-lactamases isolated from gram-negative bacteria, the interactions of cefmenoxime, a new methoxy-imino-amino-2-thiazol cephalosporin were compared with those of cefotaxime, lamoxactam, cefoperazone and ceftazidime. on-line computerized microacidimetry allowed determination of the affinity of these compounds for the enzymes, which was characterized by ki values. microacidimetry showed poor interactions of cefmenoxime with penicillinase tem-1 (low vm, poor affi ...19853939122
effects of environment on infection in burn patients.burn patients in an early cohort (n = 173) treated in an intensive care ward without separate enclosures were compared with a later cohort (n = 213) treated in a renovated unit with separate bed enclosures. the number of patients developing infection was significantly reduced in the late group. observed mortality was compared with mortality predicted on the basis of burn size and age alone. reduction in observed compared with predicted mortality, inapparent in the early group, was seen in the la ...19863942497
clinical and microbiologic consequences of amikacin use during a 42-month period.in june 1980, 23% of our pseudomonas aeruginosa isolates and 53% of our serratia species were resistant to gentamicin and tobramycin. during a 3 1/2-year period of almost exclusive amikacin usage, we noted a fall in overall resistance of gram-negative organisms to tobramycin and gentamicin from 18.8% and 19.3% to 15.2% and 16.2%, respectively. this fall in resistance was most notable for escherichia coli, proteus mirabilis, and serratia species. during this period there was no increase in amikac ...19863954526
selective and differential medium for the primary isolation of members of the proteeae.a new differential and selective medium for the isolation of members of the proteeae, pim (proteeae isolation medium) agar, was developed and evaluated. the medium relies on the ability of all members of the proteeae (with the exception of a very few morganella morganii strains) to produce a dark brown pigment in medium containing dl-tryptophan. an additional differential property, tyrosine degradation, was also demonstrated by the medium. members of the proteeae appeared as dark brown colonies ...19863958148
composition and antimicrobic resistance of skin flora in hospitalized and healthy adults.the aerobic bacterial flora of the skin of the anterior nares, axilla, perineum, and toe web in a group of 37 patients hospitalized for at least 2 weeks was compared with the flora of 30 healthy adults. colony counts were significantly higher for flora of the perineum and toe web in both groups (p less than 0.05). patients had significantly higher carriage rates of proteus, pseudomonas, and candida spp. (p less than 0.01). staphylococcus haemolyticus was isolated more frequently from patients, a ...19863958149
[presence of newberyite in the right kidney and carbonated apatite in the left kidney in a patient with recurrent urinary infections].in about 20% of the cases, urolithiasis is directly linked to chronic urinary tract infection by urea splitting bacteria, essentially proteus mirabilis. the stones are generally composed of struvite and carbonate apatite which usually occur as staghorn calculi feared because of their obstructive and infectious consequences that cause important renal damage. the case of a 48 year-old woman with chronic urinary infection is described whose right kidney showed a staghorn calculus essentially compos ...19863960256
the effect of polyvinylpyrrolidone-iodine as an disinfectant in eye surgery.105 patients admitted for cataract surgery were treated pre-operatively with polyvinylpyrrolidone-iodine (pvp-i) as a disinfectant, and consecutive bacterial cultures were obtained before initiating surgery and at the end of the operation. a control group (n = 99) only had a standard disinfection procedure of the eye (including instillation of terramycin cum polymyxin b cream in the conjunctival sac the evening before surgery). the cultures obtained from the conjunctival sac of the control group ...19863962622
studies on microorganisms secured from different organs of slaughtered sheep with special reference to the microbial load in certain muscles.a total of 312 samples collected from different organs and muscles of dead, disabled, diseased, dying, and normal carcasses of sheep were examined bacteriologically to determine the different species of microorganisms as to their public health significance. in addition, the aerobic plate count (apc) and most probable number (mpn) coliform/g were determined of musculus biceps brachii, musculus quadriceps femoris, and musculus longissimus dorsi. the mean bacterial load was higher in summer than in ...19863964203
oscillations in cell shape and size during locomotion and in contractile activities of physarum polycephalum, dictyostelium discoideum, amoeba proteus and macrophages.changes in cell shape and size were measured during locomotion, together with the motive force of the protoplasmic streaming, in various amoeboid cells in different stages of their life cycle, and under various environmental conditions. the variations in these measurements with time were examined by fourier spectral analysis. notwithstanding a change in cell type in the life cycle of p. polycephalum, myxamoebae and tiny plasmodia showed a similar time pattern of locomotion, exhibiting oscillatio ...19853965294
detection of gram-negative bacteremia by limulus amebocyte lysate assay: evaluation in a rat model of peritonitis.a spectrophotometric limulus amebocyte lysate assay using lysis filtration and centrifugation has been developed for the detection of gram-negative bacteria in blood. the assay is directed at detection of endotoxin in viable and nonviable bacteria present in the blood-stream and not detection of free endotoxin in plasma. the assay was evaluated in a model of peritonitis in which rats were challenged with an inoculum consisting of sterilized human feces, barium sulfate, and one of eight species o ...19853965586
the spot indole test for identification of swarming proteus.the authors evaluated the use of the spot indole test for rapid speciation of swarming proteus from the primary isolation plate. one hundred seventy-two consecutive isolates of swarming proteus were studied, 163 proteus mirabilis and nine proteus vulgaris. one hundred fifty-six isolates (95.7%) of proteus mirabilis gave a negative spot indole. seven (4.3%) gave a positive spot indole test, but all seven isolates were from cultures in which other indole-producing organisms also were present. if o ...19853966445
cell-mediated immunity in schoolchildren assessed by multitest skin testing. normal values and proposed scoring system for healthy children.measurement of cutaneous delayed-type hypersensitivity (dth) to a battery of ubiquitous antigens is an accepted means of assessing cell-mediated immunity (cmi). the recently introduced multitest cmi system consists of a plastic multiple puncture device that simultaneously applies seven standardized recall antigens in a reproducible manner. a representative population of 448 healthy us schoolchildren was tested to determine incidence and size of dth responses to each of the seven antigens. all re ...19853976586
the risk of bladder calculi in patients with spinal cord injuries.a nonconcurrent prospective study of bladder calculi included 500 persons treated at the university of alabama in birmingham spinal cord injury care system between 1973 and 1981. risk factors suspected of contributing to the development of bladder calculi were identified. logistic regression analysis was used to estimate each risk factor's adjusted odds ratio and to develop a predictive model for bladder stone formation. bladder calculi were most likely to develop within one year of injury. pati ...19853977510
analysis of fifty cases of facial palsy due to otitis media.fifty cases of facial palsy (fp) due to otitis media treated in our hospital during the 10-year period from 1972 to 1981 were analyzed. the averaged percentage of this incidence was 3.1% of all the fp occurring. fp due to otitis media acuta (oma) was frequently seen in infants. fp due to otitis media chronica (omc) tended to occur in combination with cholesteatoma or acute exacerbations of infection. paralyses were generally mild and bacteria from the otorrhea were similar to those of omc withou ...19853977768
[clinical characteristics of l-form bacteremic septicemia. report of 5 cases]. 19853979184
the development of a formulation for radiation-sterilizable urea broth.urea broth, a medium used for the identification of the genus proteus, was sterilized by gamma radiation, using radiation doses of 1-1.5 mrad. the radiation-sterilized medium, modified by adding sodium ascorbate and increasing its phenol red and yeast extract content, performed as well as the commercial formulation prepared aseptically, when tested with different proteus and non-proteus species. gamma-irradiation appears to be an attractive and economical method for sterilizing nutrient media in ...19853980295
[urinary lithiasis in children. apropos of 92 cases].the charts of 92 children with urinary calculi were reviewed. there were 69 boys (75%) and 23 girls (25%), 20 (22%) were infants. calculi were in kidney (74 times but were often multifocal. fourteen children (15%) presented with a lithogenic metabolic disorder, 28 (30%) had a malformation of the urinary tract, in 4 (4.3%) a permanent urinary derivation had been performed for the treatment of a malformation or a neurologic bladder. the other children presented with apparently idiopathic lithiasis ...19853985751
bacterial resistance to antibiotics in american hospitals.a total of 5,825,243 strains of bacteria were isolated in 242 american hospitals during a period of twelve years (1971 to 1982). the susceptibility to antibiotics of escherichia coli, staphylococcus aureus, klebsiella sp. proteus mirabilis, haemophilus sp. and pseudomonas sp. did not change significantly during this period of time. the susceptibility to tetracycline for e. coli and s. aureus increased. streptococcus faecalis and staphylococcus epidermidis showed a decrease in susceptibility.19853986941
dynamics of resistance to cephalosporins.bacterial susceptibility to various cephalosporins of nine of the most frequently isolated organisms were analyzed for the five-year period 1980 to 1984. the disk diffusion susceptibility to cephalosporins of 1,640,031 strains of bacteria isolated from 242 hospitals in the united states were compared. no significant change has occurred in the susceptibility of escherichia coli, klebsiella pneumoniae, proteus mirabilis, haemophilus influenzae, or streptococcus faecalis for this period; enterobact ...19853986942
variability in the proteus syndrome: report of an affected child with progressive lipomatosis.in 1983 the proteus syndrome was delineated by wiedemann et al. [12]. we report a 10-month-old girl, a further child affected by the new syndrome. the typical signs are macrodactyly, hemihypertrophy, pigmented nevi, hyperkeratosis, and subcutaneous hamartomatous tumours. our patient shows an aggressive lipomatosis on the trunk and local relapses after surgical interventions in the regions involved. histology of the adipose tissue showed considerable anisocytosis and increased cell volume.19853987735
[effects of human urinary trypsin inhibitor against operative stress].effects of human urinary trypsin inhibitor (mti) against operative stress were investigated. laparotomy in a mouse caused suppression of immunological functions such as phagocytic activity and antibody formation, followed by loss of resistivity to bacterial infection and acceleration of growth of concealed tumor. the operation also caused damages to the body such as enhancement of protein catabolism and suppression of renal function, followed by increase of blood urea nitrogen, increase of prote ...19853988164
disseminated cutaneous squamous cell carcinoma in a ferret.a 3-year-old male ferret was admitted with ulcerated nodules and fistulous draining tracts on the pads and digits of the right front and left hind feet. proteus sp and escherichia coli were isolated from the exudate. histologic examinations revealed that all skin lesions were associated with an underlying squamous cell carcinoma. surgical removal was attempted, followed by chemotherapy. regression of remaining lesions was noted before discharge; however, the ferret died 5 days later and was not ...19853988603
assessment of head-space gas-liquid chromatography for the rapid detection of growth in blood cultures.blood for transfusion was inoculated with between 10(0) and 10(2) colony-forming units (cfu) per ml of each of 59 microbial isolates and added to cooked meat broth. at intervals up to 72 h incubation, the cultures were examined by conventional visual inspection and automated head-space gas-liquid chromatography (hs-glc). forty-six isolates including all those examined of staphylococcus aureus, streptococcus pyogenes, s. pneumoniae, s. faecalis, s. milleri, s. mitior, s. mitis, s. salivarius, s. ...19853988854
[in vitro antibacterial activity of various cephems against clinical isolates from complicated urinary tract infections].in vitro antibacterial activity of several cephems (cez as the first generation (group a); ctm and cmz as the second generation (group b); cmx, cpz, lmox, ctx and czx as the third generation (group c)) against 8 species, each of 54 strains, of gram-negative clinical isolates from complicated urinary tract infection was compared by determination of the mics. the following results were obtained: the most sensitive drugs against each species in mic80; ctx (mic80 0.20 microgram/ml) against e. coli, ...19853989977
photophobic and phototactic responses of amoeba proteus in kcn and sham solutions.the effects of the metabolic inhibitors kcn and sham on phototaxis and photophobic response in amoeba proteus have been studied. both drugs neither change amoebae photophobic response nor the phototactic reaction. the results indicate clearly that the negative phototactic orientation is not impaired by impediment of respiration thus it is not directly coupled to the differences in energy production in different parts of a. proteus body.19853995598
purification and properties of a pyridoxal 5'-phosphate-dependent histidine decarboxylase from morganella morganii am-15.a pyridoxal 5'-phosphate-dependent histidine decarboxylase from morganella morganii am-15 was purified to homogeneity. the enzyme is a tetramer (mr 170,000) of identical subunits and binds 4 pyridoxal-p/tetramer; it is resolved by dialysis against cysteine at ph 6.8. between ph 6.2 and 8.8, the holoenzyme shows ph-independent absorbance maxima at 333 and 416 nm. vmax/km is highest at ph 6.5; this optimum reflects chiefly increased km values for histidine at lower or higher ph values, whereas vma ...19853997848
[empiric therapy with piperacillin plus amikacin for febrile patients with cancer--with emphasis on granulocytopenia].between august 1979 and december 1983 one hundred episodes of fever in 70 patients with cancer, most of whom had granulocytopenia, were treated empirically with a combination of piperacillin (4.0g, every 6 hours) and amikacin (200mg, every 6 hours) at saitama cancer center. profound granulocytopenia (less than 100/mm3 of granulocytes) was present at the beginning of treatment in 40% of episodes. oral absorbable or nonabsorbable antibiotics were used in 57 febrile episodes. wbc transfusion was no ...19854004291
screening of microorganisms for nitrosation catalysis at ph 7 and kinetic studies on nitrosamine formation from secondary amines by e. coli strains.thirty-eight strains of microorganisms isolated from infected human trachea, urine, blood and faeces were examined for their ability to form n-nitrosomorpholine from morpholine and nitrite at ph 7.25. twenty-five bacterial strains exhibited nitrosation activity, including 18 out of 19 strains of escherichia coli and three out of nine pseudomonas aeruginosa, proteus morganii, proteus mirabilis, klebsiella pneumoniae and neisseria strains; e. coli a10 strain showed the highest activity. formation ...19854006079
interaction of subminimal inhibitory concentrations of clindamycin and gram-negative aerobic organisms: effects on adhesion and polymorphonuclear leukocyte function.the effect of pre-incubation of pseudomonas aeruginosa, klebsiella pneumoniae and proteus mirabilis with sub-inhibitory concentrations (sub mics) of clindamycin on the adherence of these organisms was studied. culturing these organisms in the presence of clindamycin (4 mg/l) resulted in significant enhancement of adherence for ps. aeruginosa and pr. mirabilis and decreased adherence for k. pneumoniae. furthermore, the effect of pre-exposure to clindamycin on polymorphonuclear leukocyte (pmnls) f ...19854008380
lateral flagellar antigen of vibrio alginolyticus and vibrio harveyi: existence of serovars common to the two species.the antigenicity of lateral (l-) flagella of two marine vibrios, vibrio alginolyticus and v. harveyi, was studied, and the two species were found to have common antigenicity of their flagella. antisera against l-flagella were prepared by immunizing rabbits with highly purified l-flagellar filaments. h-agglutination tests with the anti-l-flagella antisera showed that four h-serovars existed in these species and that two of them were shared by the two species. cross reactivity between h-serovars o ...19854010543
[otogenic intracranial complications--an ever-present problem]. 19854011268
nosocomial infection of urinary tract: changing pathogens, changing patterns.risk factors for the development of nosocomial infection, i.e., diabetes, immunosuppressive therapy, etc., are reviewed. in most cases, the patient's own fecal flora is the primary reservoir for potentially infecting pathogens, such as escherichia coli, pseudomonas, klebsiella, enterobacter, proteus, serratia, and enterococcus. hospitalized patients are likely to have antibiotic-related changes in fecal flora. abnormal urethral flora in men, as well as high rates of vaginal and urethral coloniza ...19854012997
[renal angiography following anatrophic nephrotomy in coralliform lithiasis]. 19854013847
the antagonism of tetracycline and ferric iron in vivo.to test the hypothesis that the in-vivo antibiotic action of tetracycline might be affected by ferric iron and the enhancement of infection by ferric iron by tetracycline, the actions of intraperitoneal antibiotic and local ferric ammonium citrate, given separately and together, were measured in the dorsal skin of guinea-pigs bearing lesions due to staphylococci, streptococci, a proteus sp., an erysipelothrix sp., clostridium perfringens, pseudomonas aeruginosa, aeromonas hydrophila and klebsiel ...19854020849
bacteria and food poisoning organisms in milk.individual milk samples of 50 goats, 50 ewes and 50 cows were examined for the total viable count, coliform bacteria, staphylococci and salmonellae. growth of enterotoxin a producing staphylococcus aureus nctc 10652 in the milk of the three animal species was also studied. the average total count was 1.9 x 10(7) cells/ml for cow's, 7.7 x 10(6) for goat's and 2.7 x 10(6) for ewe's milk with micrococci staphylococci, rods and streptococci being the predominant organisms in the three milks, respect ...19854022103
on percutaneous drainage of retroperitoneal collections: when is primary surgical drainage preferable?percutaneous drainage of retroperitoneal collections is a method employed with an ever-increasing frequency. the indication for primary surgical drainage of these collections is rapidly decreasing. herein we describe what we consider to be the indications for primary surgical drainage of retroperitoneal collections illustrated by the recurrence of the abscess in 3 of our patients following adequate primary percutaneous drainage.19854024400
[treatment of acute and chronic urological infections with azlocillin--clinical and microbiological research].the authors present their results from the treatment of urological infections with the new semisynthetic penicillin antibiotic azlocillin (acyl-ureide-penicillin) in 86 patients with various, in kind and clinical manifestations, infections of the urinary system. the susceptibility was determined of the isolated bacterial causative agents of the urological infections to azlocillin and to the other antibacterial preparations available in our country. it was established that 52,7% of gram-negative ...19854024609
[microbial spectrum in chronic pyelonephritis and the drug sensitivity of the microorganisms].the authors studied the etiological spectrum of 226 patients with chronic pyelonephritis, with 926 microbic strains isolated from the urocultures. the most frequent microbic causative agents were e. coli (40,82%), proteus (a total of 17,82%), enterobacter (8,86%), klebsiella (8,64%), pseudomonas (6,16%), enterococcus (5,94%). the drug susceptibility of the majority of the isolated gram-negative microorganisms versus ampicillin and chloramphenicol, widely used in out-patient department practice, ...19854024620
[causative agents of urologic infections and their sensitivity to the most frequently used antibiotics and chemotherapeutic agents (data from the clinic for the therapy of internal diseases and clinical pharmacology for 1984)].the causative agents of uroinfections were studied among the patients at the clinic of therapy of internal diseases and clinical pharmacology during 1984. significant bacteriuria was established in 279 out of 485 patients, with 1475 urine inoculations performed, 14 with mixed flora. the highest is the share of e. coli--50,5 per cent, followed by proteus--25,7 per cent. no essential change was established in the microbiological spectrum of the causative agents of uroinfections as compared with th ...19854024621
cell-mediated immunity assessed by multitest cmi skin testing in infants and preschool children.two hundred twenty-one healthy children, from 6 months to 7 years of age, were tested for delayed-type hypersensitivity (dth) by the multitest cmi (cell-mediated immunity) (merieux institute, miami). this device permits the simultaneous application of seven standardized recall antigens and a glycerol diluent control. younger children were tested on the back and older children on the volar surface of the forearm. only 6.8% of the children were anergic, and most of them (11/15) were female. the dt ...19854025264
aminoglycoside resistance in gram-negative bacilli during increased amikacin use. comparison of experience in 14 united states hospitals with experience in the minneapolis veterans administration medical center.resistance to amikacin, gentamicin, and tobramycin was surveyed prospectively during controlled aminoglycoside usage in 14 hospitals. following an initial baseline period (minimum, three months) during which gentamicin use predominated, gentamicin and tobramycin were placed on restrictive control, establishing amikacin as the aminoglycoside of general use (86 percent of total aminoglycoside usage). during an average of 35 months' restriction of gentamicin and tobramycin, significant reductions i ...19854025364
treatment of pediatric infections with amikacin as first-line aminoglycoside.because of increased aminoglycoside resistance of hospital bacterial isolates, aminoglycoside sensitivity patterns of isolates in a large children's hospital were assessed before and during a 33-month period of almost exclusive amikacin use. there was no significant change in overall resistance rates of gram-negative enteric bacteria to gentamicin (4.8 percent and 4.6 percent), tobramycin (2.5 percent and 3.6 percent), and amikacin (1.2 percent and 1.8 percent) from the pre-amikacin period to th ...19854025367
in vitro comparative antimicrobial activity of cefpimizole against clinical isolates from five medical centers.cefpimizole was compared with cefoperazone and cefotaxime against 6,599 clinical bacterial isolates from five medical centers. cefoperazone and cefotaxime were both more active and provided a greater spectrum of antimicrobial coverage than cefpimizole. some of the cefpimizole minimum concentrations inhibiting 50% of tested strains were as follows: citrobacter freundii and enterobacter cloacae, 16 micrograms/ml; escherichia coli and klebsiella pneumoniae, 2.0 micrograms/ml; proteus mirabilis, 1.0 ...19854026275
comparative in vitro activity of temocillin.the antibacterial activity of temocillin, a novel beta-lactam antibiotic, was tested against 796 clinical isolates. we also conducted a comparative study against 8 other antibiotics. temocillin exhibited good activity against gram-negative organisms including escherichia coli, and the genera proteus, enterobacter, serratia, klebsiella, citrobacter, providencia, salmonella, shigella and haemophilus: 98% of the strains were inhibited by concentrations less than or equal to 16 mg/l. the results of ...19854029029
role of protein subunits in proteus rettgeri penicillin g acylase.penicillin g acylase from proteus rettgeri is an 80,000- to 90,000-dalton enzyme composed of two nonidentical subunits. both subunits were required for enzymatic activity. the 65,000-dalton beta subunit contained a phenylmethylsulfonyl fluoride-sensitive residue required for enzymatic activity, and the 24,500-dalton alpha subunit contained the domain that imparts specificity for the penicillin side chain.19854030697
automated reading of mic microdilution trays containing fluorogenic enzyme substrates with the sensititre autoreader.the sensititre autoreader is a microcomputer-driven instrument capable of automatically reading antimicrobial susceptibility microdilution trays. the instrument measures the fluorescence liberated by bacterial enzymatic activity on fluorogenic substrates as an indicator of growth in each well. a mathematical algorithm converts the fluorescent signals from an antimicrobial dilution series to an mic endpoint. a three-center study evaluated the performance of the autoreader in comparison with mic d ...19854031033
a 12-month fever surveillance study in a veterans' long-stay institution.this report describes a 12-month fever surveillance survey in a 258-bed veterans long-term care institution. there were 128 episodes of fever (one episode per 24 patient-months); 114 were studied. lower respiratory tract infections were most frequent, 36 (32%), with 26 (23%) urinary tract infections. streptococcus pneumoniae was the most common pathogen in the chest infections and proteus mirabilis the most common of the urinary tract infections. in 40 (35%) there was no evidence of a lower resp ...19854031336
[pulmonary nocardiosis. apropos of a case]. 19854033256
pulmonary cavitation following pulmonary infarction.cavitation following bland pulmonary infarction is not commonly considered in the differential diagnosis of cavitary lung disease. in a 4-year period we have found 10 cases of cavitating pulmonary infarction (cpi) by reviewing serial chest radiographs from autopsies with pulmonary infarction and in all cases with positive ventilation-perfusion lung scans. we have compared these cases to 31 previously reported cases in the english literature that met our criteria for cpi. in our 10 patients, ther ...19854033411
[effectiveness of using maliutka and malysh adapted propionic-acidophilus mixtures in the combined treatment of congenital hypotrophy].the physical and psychomotor development, immune responsiveness, and intestinal microflora were studied in 82 infants with congenital hypotrophy depending on the nutrition pattern. it was shown that children who received the propionic-acidophilic mixtures malyutka and malush manifested the highest daily weight gain, the most rapid disappearance on the manifestations of food allergy, an increase in immune responsiveness. a decrease in the cultivation rate of proteus vulgaris and pathogenic staphy ...19854036072
clinical pharmacokinetics of amikacin in dogs.after iv, im, and subcutaneous injection of single dosages of amikacin (5, 10, and 20 mg/kg of body weight) in each of 4 dogs, the elimination kinetics of amikacin were determined. the pattern of urinary excretion and cumulative amount excreted unchanged in 24 hours were also determined. amikacin had a short half-life (approx 1 hour) that was independent of the dosage. intravenous injection of 10 mg/kg gave apparent volume of distribution of 226 +/- 37 ml/kg and body clearance of 2.64 +/- 0.24 m ...19854037511
use of aztreonam in the treatment of serious infections due to multiresistant gram-negative organisms, including pseudomonas aeruginosa.aztreonam is a novel antimicrobial agent belonging to the monobactam class of antibiotics. it inhibits both beta-lactamase-producing and non-beta-lactamase-producing aerobic gram-negative bacilli, but it has no activity against gram-positive species or against anaerobic species. the efficacy of aztreonam in the treatment of infection in 76 patients and its safety in 87 patients was evaluated. the majority (91 percent) of patients had significant underlying disease, and 47 percent were critically ...19854038574
in vitro activity of aztreonam--a new monocyclic beta-lactam antibiotic.the in vitro antibacterial activity of aztreonam (sq 26776), a new beta-lactam antibiotic, was measured by the agar dilution technique. aztreonam is known to have a narrow spectrum with activity only against gram negative bacteria. the strains tested were 223 clinical isolates from blood cultures obtained at rigshospitalet, copenhagen, denmark. its activity against e. coli, klebsiella spp., proteus spp., enterobacter spp., citrobacter, salmonella typhimurium and serratia marcescens was satisfact ...19854040456
concrement formation in the urinary bladder in rats inoculated with ureaplasma urealyticum.to study the concrement-forming ability of ureaplasma urealyticum in the urinary tract, viable and heat-killed ureaplasmas as well as urease and non-urease-producing bacteria were inoculated into the bladder in rats. viable ureaplasmas, in contrast to heat-killed, caused the formation of bladder stones with a frequency corresponding to urease-producing bacteria (proteus mirabilis). it was not possible to reculture the inoculated ureaplasmas from the urinary tract. non-urease producing microorgan ...19854049607
[early diffuse hypertrophic osteitis recurrence. unexpected and consternating development after a large évidement cavity. apropos of 15 cases].the authors report 15 cases collected over a period of 13 years (1972 - 1984) of a particularly serious eventuality affecting certain evacuation cavities: early recurrence of diffuse hypertrophic osteitis (e.r.d.h.o.). this is a rare complication, occurring in 1.5% of cases of chronic otitis. however, the fact of having operated upon 7 cases in 1984 alone, whilst during the previous 12 years, only 8 had been seen, raised the alarm. the classical pattern of this complication occurs in 3 stages, e ...19854051389
[electrophoretic polymorphism of lactate, malate and glutamate dehydrogenases, acid phosphatase and esterases of providencia alcalifaciens, p. stuartii and p. rustigianii].the polymorphism of glutamate, lactate and malate dehydrogenases, of acid phosphatase and of esterases of 27 strains of providencia alcalifaciens, 35 strains of p. stuartii and 17 strains of p. rustigianii was investigated by conventional electrophoresis in polyacrylamide agarose gel and by isoelectric focusing in thin-layer polyacrylamide gel. for each enzyme analysed, the three species were characterized by a distinct electrophoretic pattern. the number of allozymes detected by conventional el ...19854051454
computed tomography of xanthogranulomatous pyelonephritis presenting as a flank mass.xanthogranulomatous pyelonephritis should be part of the differential diagnosis when extensive soft tissue involvement is associated with renal disease. as a diagnosis of neoplasm may be rendered even on microscopy, it is particularly important that this entity be considered.19854053662
impact of injectable cephalosporins on the gastrointestinal microflora: observations in healthy volunteers and hospitalized patients.a disturbed microbiological ecosystem of the gut flora is frequently seen as a consequence of antibiotic therapy. because this impact on the physiological balance is known to be causative for severe nosocomial infections and is mainly seen with antibiotics that are massively excreted via the bile (e.g. broadspectrum penicillins, ceftriaxone and cefoperazone), we investigated cefotaxime (ctx), cefotiam (ctm), cefmenoxime (cmx), ceftazidime (caz), ceftizoxime (czx) and cefazolin + netilmicin (cez ...19854055042
variable phenotypes of providencia stuartii due to plasmid-encoded traits.weekly urine specimens from 51 long-term catheterized patients yielded 699 isolates of providencia stuartii. urease-positive strains represented 23.7% (166) of the isolates, sucrose-positive strains represented 24.5% (171), and lactose-utilizing strains represented 0.7% (5). urease and sucrose traits were transferred by conjugation to escherichia coli via an 82-kilobase plasmid; lactose fermentation was transferred by a 150-kilobase plasmid.19854056008
an epidemiological survey of urinary tract infections among outpatients in northern norway.urinary tract infections (uti) in all outpatients from several municipalities in northern norway were examined during 1 year. comparatively high frequencies of uti were found among women of all ages, among people greater than 60 years of both sexes, in coastline municipalities, and during the fall and winter. about 70% of all uti were caused by escherichia coli, more in women than in men. other gram-negative enterobacteria (klebsiella, proteus) and gram-positive cocci (staphylococcus epidermidis ...19854059868
serial delayed cutaneous hypersensitivity skin testing with multiple recall antigens in healthy volunteers: booster effect study.booster effects on delayed cutaneous hypersensitivity (dch) responses have been demonstrated for various antigens when dch is measured by the mantoux technique. in the present study, we investigated this possibility when assessing dch responses using the multitest cmi multipuncture technique with simultaneous injections of seven test antigens and a control. the dch responses were quantified for each antigen and for the overall dch response expressed as a dch score. in a group of healthy voluntee ...19854061981
antibacterial activity of amifloxacin (win 49, 375), a new quinolone agent.the in vitro activity of amifloxacin, a quinolone antimicrobial agent was compared with those of ciprofloxacin, enoxacin, ofloxacin and norfloxacin against gram-positive and gram-negative bacteria. ninety percent of escherichia coli, klebsiella species, aeromonas, salmonella, shigella, citrobacter, enterobacter species, proteus mirabilis, serratia marcescens, and morganella morganii were inhibited by less than or equal to 0.5 microgram/ml. amifloxacin inhibited branhamella, haemophilus, and neis ...19854064609
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