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psoas abscess twenty-one years after ipsilateral nephrectomy.we report an unusual case of psoas abscess, which developed twenty-one years after ipsilateral nephrectomy and was caused by infrequent pathogen, proteus mirabilis. it was diagnosed by computed tomography and was drained percutaneously with a nephrostomy tube guided by ultrasonography.200212899219
diagnosis, treatment and registration of urinary tract infections in geriatric patients.bacteriuria (> or = 10(5) cfu/ml) is a very common phenomenon in elderly people, occurring twice as frequently in women than in men. there are symptomatic and asymptomatic types of bacteriuria. risk factors include: a decrease in the estrogen level in women after the menopause, catheterisation, urinary bladder dysfunction, hypertrophy of the prostate gland, diabetes, neurological illnesses. the diagnosis of bacteriuria is based on quantitative urine culture (positive result--> or = 10(5) cfu/ml ...200212898972
asymptomatic bacteriuria in patients on continuous ambulatory peritoneal dialysis.chronic inflammatory state and chronic infections appear to be one of the most important risk factors of morbidity and mortality in patients (pts) on dialysis. the aim of our study was to determine the frequency of urinary tract infections (uti) in clinically asymptomatic patients on capd. the study included 43 (22f, 21m) clinically stable asymptomatic capd patients with diuresis ranged from 300 to 2500 ml per 24 h. clean catch urine specimen, was cultured and identified by standard technique. u ...200212898851
diverse effects of ascorbic acid and palmitoyl ascorbate on helicobacter pylori survival and growth.among many antioxidants used in the food, pharmaceutical and cosmetic industries, ascorbic acid (aa) is one of the most important. aa has been suggested to decrease the risk of gastric disease (gastritis, duodenal ulcer, and carcinoma) by direct action on helicobacter pylori. however, there are limited studies on the possible role of aa and its derivatives such as palmitoyl ascorbate (pa) on the growth and survival of h. pylori. in the present study it was demonstrated in vitro that aa in the co ...200312892889
polypoid cystitis in 17 dogs (1978-2001).polypoid cystitis is a rare disease of the urinary bladder in dogs characterized by inflammation, epithelial proliferation, and development of a polypoid mass or masses without histopathologic evidence of neoplasia. medical records of 17 dogs with polypoid cystitis were reviewed to determine the clinical and laboratory features of this disorder and to assess treatment and outcome. most affected dogs (15/17) were female and presented for evaluation of hematuria or recurrent urinary tract infectio ...200312892301
[some specific features of vaginal microflora depending on ecological factors].in 75 female residents of vladikavkaz, aged 18-45 years, who had applied to the gynecological department with complaints of vaginal discharge, the qualitative and quantitative composition of vaginal microflora was studied. all these women were divided into 2 groups: group 1 including 38 women living in ecologically unfavorable districts of the city and group 2 including 37 women living under ecologically favorable conditions. the relationship between the ecological situation of the districts of ...200312886612
target affinities of faropenem to and its impact on the morphology of gram-positive and gram-negative bacteria.faropenem is a new oral beta-lactam antibiotic unique from carbapenems and other available beta-lactams. determinants of the in vitro activity of beta-lactam antibiotics include affinity to penicillin-binding proteins (pbps) and beta-lactamase stability. in this study, the binding affinity of faropenem to various pbps and its impact on the morphology of staphylococcus aureus and escherichia coli were evaluated. in general, faropenem demonstrated high binding affinity to high-molecular-weight pbp ...200312886052
polymer surface properties and their effect on the adhesion of proteus mirabilis.a problem encountered in patients undergoing long-term catheterization of the urinary tract is that of encrustation and blockage of the catheter by crystalline bacterial biofilms. this is principally caused by the action of the urease-producing pathogen proteus mirabilis. a major aim of this work is to develop materials resistant to encrustation. here, the effects of polymer surface properties on the adhesion of p. mirabilis are examined. spin-coated polymer films were characterized through cont ...200312885198
candida--agent of the diaper dermatitis?occurrence of candida spp. was determined in a population of 60 infants, 1-15-month-old, with diaper dermatitis, admitted to a neonatal intensive care unit in hospital saca (kosice, slovakia). specimens were obtained from the perianal, pubic, inguinal, or gluteal areas that showed signs of secondary infection as manifested by erythema, oozing, vesiculopustular lesions, and pus formation. the most frequently isolated species was c. albicans (41), followed by c. parapsilosis (8), c. tropicalis (4) ...200312879751
in vitro and in vivo activities of novel 2-(thiazol-2-ylthio)-1beta-methylcarbapenems with potent activities against multiresistant gram-positive bacteria.sm-197436, sm-232721, and sm-232724 are new 1beta-methylcarbapenems with a unique 4-substituted thiazol-2-ylthio moiety at the c-2 side chain. in agar dilution susceptibility testing these novel carbapenems were active against methicillin-resistant staphylococcus aureus (mrsa) and staphylococcus epidermidis (mrse) with a mic(90) of </=4 micro g/ml. furthermore, sm-232724 showed strong bactericidal activity against mrsa, in contrast to linezolid, which was bacteriostatic up to four times the mic. ...200312878507
tolerance to various toxicants by marine bacteria highly resistant to mercury.bacteria highly resistant to mercury isolated from seawater and sediment samples were tested for growth in the presence of different heavy metals, pesticides, phenol, formaldehyde, formic acid, and trichloroethane to investigate their potential for growth in the presence of a variety of toxic xenobiotics. we hypothesized that bacteria resistant to high concentrations of mercury would have potential capacities to tolerate or possibly degrade a variety of toxic materials and thus would be importan ...200312876655
[purple urine bag syndrome].the report of purple discoloration in a urinary drainage system, known as purple urine bag syndrome (p.u.b.s.) is rarely described in the literature.200312876527
proteus syndrome associated with liver involvement: case report.we report a patient with proteus syndrome who has epidermal nevus, right-sided asymmetric growth of extremities, pelvis, vertebrae and hemimegalencephaly. this patient also had enlargement of the liver which is not reported before in the proteus syndrome.200312872817
[pediatric vesical lithiasis. 70 case reports].the study focuses on 70 vesicals lithiasis, with a sample consisting of 60 boys (86%) and ten girls (14%). the age varies from one year to 14 years. the revealing signs are dominated by mictional troubles in 47 cases (67%), macroscopic hematurie in 26 cases (37%), the pyurie in 12 cases (17%) and acute retention of urine in 7 cases (10%). the most frequent germs are the staphylococcus aureus with 28% of cases, the protéus mirabilis with 24% of cases and the echerichia coli with 24% of the cases. ...200312872601
enhanced microbial biomass assay using mutant luciferase resistant to benzalkonium chloride.in a biomass assay based on adenosine 5(')-triphosphate (atp) bioluminescence, extracellular atp is removed; then intracellular atp is extracted from the microorganism by an atp extractant and subsequently reacted with luciferase. to provide a highly sensitive assay, the concentration of benzalkonium chloride (bac) in the atp extractant was optimized by using a mutant luciferase resistant to bac. the use of 0.2% bac, which was acceptable for the luciferase, simultaneously achieved the maximum ex ...200312871724
structure of the o-polysaccharide from proteus myxofaciens. classification of the bacterium into a new proteus-o-serogroup.the o-polysaccharide (o-antigen) was obtained from the lipopolysaccharide of proteus myxofaciens, a proteus strain producing copious amounts of slime, which was isolated from the gypsy moth larvae. the structure of the polysaccharide was studied by chemical analysis and 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, roesy and h-detected 1h,13c hmqc experiments. it was found that the polysaccharide contains an amide of glucuronic acid (glca) with an unusual alpha-linked amino acid, nepsil ...200312869193
ampc beta-lactamase producing multidrug resistant strains of klebsiella spp. & escherichia coli isolated from children under five in chennai.ampc beta-lactamases are group i cephalosporinases that confer resistance to a wide variety of beta-lactam drugs. plasmid mediated ampc beta-lactamases has been discovered most frequently in isolates of klebsiella pneumoniae, k. oxytoca, salmonella, proteus mirabilis and escherichia coli. the present study was undertaken to study the occurrence of multidrug resistant and ampc beta-lactamase producing klebsiella spp. and escherichia coli in children less than five years of age as this age group i ...200312866821
antiviral activity of n-benzoylphenylisoserinates of lactarius sesquiterpenoid alcohols in vitro.cytotoxic, antiviral, antibacterial and antifungal properties of a new, originally synthesised group of compounds: the n-benzoylphenylisoserinates of sesquiterpenoid alcohols - derivatives of taxol and various sesquiterpenes of lactarius origin were evaluated in vitro. among 16 compounds tested, 6 decreased hsv-1 titres. selectivity indices ranged from 13.9 to 31.7. no activity against rna viruses (parainfluenza 3, coxsackie b3, vesicular stomatitis virus, and encephalomyocarditis virus), bacter ...200312865976
clinical quiz. d proteus syndrome. 200312862023
real-time pcr duplex assay for rickettsia prowazekii and borrelia recurrentis.rickettsia prowazekii, the etiologic agent for epidemic typhus, and borrelia recurrentis, the etiologic agent of relapsing fever, both utilize the same vector, the human body louse (pediculus humanus), to transmit human disease. we have developed an assay to detect both bacterial pathogens in a single tube utilizing real-time pcr. assays for both agents are specific. the r. prowazekii and b. recurrentis assays do not detect nucleic acid from r. typhi, r. canada, or any of eight spotted fever ric ...200312860643
biogenesis of melanosomes in kupffer cells of proteus anguinus (urodela, amphibia).the ultrastructural characteristics of melanosomes and premelanosomes observed during the biogenesis of melanosomes in liver pigment cells of the neotenic cave salamander proteus anguinus (proteidae) are described. it is well known that amphibian liver pigment cells, also known as kupffer cells (kc), contain melanosomes and are able to synthesize melanin. liver pigment cells of p. anguinus contain numerous siderosomes and melanosomes. the melanosomes are grouped together within single-membrane-b ...200312859617
a hospitalwide intervention program to optimize the quality of antibiotic use: impact on prescribing practice, antibiotic consumption, cost savings, and bacterial resistance.several findings from argentina provide compelling evidence of the need for more rational use of antimicrobial agents. thus, a multidisciplinary antimicrobial treatment committee for the development of a hospital-wide intervention program was formed to optimize the quality of antibiotic use in hospitals. four successive steps were developed during 6-month periods: baseline data collection, introduction of a prescription form, education, and prescribing control. sustained reduction of drug consum ...200312856209
[bladder rupture caused by spontaneous perforation of an infected urachal cyst].anomalies of the fetal urachus are rare. normally, the postnatal urachus presents as a fibrous band extending from the bladder to the umbilicus. urachal cysts may occur in postnatal life. spontaneous perforation of urachal cysts is a very rare condition, which clinically may not be distinguishable from other acute abdominal conditions. we report a case of a 63-year-old male with a history of recurrent urinary tract infections and a bladder rupture caused by a spontaneous perforation of an infect ...200312851776
the influence of inadequate empirical antimicrobial treatment on patients with bloodstream infections in an intensive care unit.to determine the occurrence of inadequate antimicrobial therapy among critically ill patients with bacteremia and the factors associated with it, to identify the microorganisms that received inadequate antimicrobial treatment, and to determine the relationship between inadequate treatment and patients outcome.200312848754
antibacterial activity of lactose-binding lectins from bufo arenarum skin.amphibians respond to microbial infection through cellular and humoral defense mechanisms such as antimicrobial protein secretion. most humoral defense proteins are synthetized in the skin. in this study we isolated two beta-galactoside-binding lectins with molecular weights of 50 and 56 kda from the skin of bufo arenarum. these lectins have significant hemagglutination activity against trypsinized rabbit erythrocytes, which was inhibited by galactose-containing saccharides. they are water-solub ...200312847913
in vitro activity of piperacillin/tazobactam and other broad-spectrum antibiotics against bacteria from hospitalised patients in the british isles.piperacillin/tazobactam is used for empirical therapy of severe and complex infections. we assessed its activity, 9 years after launch, against consecutive, clinically significant isolates from in-patients in uk and ireland. standardised disc susceptibility tests were performed on 5031 isolates at 28 hospitals. for quality assurance purposes, 5% of these isolates were collected centrally for mic tests, as were those with exceptional resistances. compared with a similar pre-launch survey in 1991, ...200312842324
isolation of proteus mirabilis from severe neonatal sepsis and central nervous system infection with extensive pneumocephalus. 200312836017
encephalocraniocutaneous lipomatosis (haberland syndrome) with bilateral cutaneous and visceral involvement.encephalocraniocutaneous lipomatosis, or haberland syndrome, is a rare congenital neurocutaneous disease. it is characterized clinically by unilateral lipomatous hamartomata of the scalp, eyelid, and outer globe of the eye, ipsilateral porencephalic cysts with cortical atrophy, cranial asymmetry, marked developmental delay and mental retardation. this syndrome should be distinguished from other mosaic neurocutaneous phenotypes such as as delleman syndrome, schimmelpenning syndrome, goltz syndrom ...200312823300
antimicrobial resistance patterns among urine isolates from patients in a geriatric hospital and from older patients in a general hospital in jerusalem.urinary tract infection is the most common cause of nosocomial infections in patients hospitalized in chronic care facilities. the changing spectrum of microorganisms involved in urinary tract infections and the emerging resistance require continuous monitoring to provide crucial information to guide empirical therapy and encourage prudent use of antibiotics. the aim of this survey was to assess the antimicrobial resistance profile of urine isolates from patients hospitalized in a geriatric hosp ...200112812604
bioactivity of secoiridoid glycosides from centaurium erythraea.as part of our on-going search for bioactive compounds from scottish plants, two secoiridoid glycosides, swertiamarin and sweroside, have been isolated from the aerial parts of centaurium erythraea rafn (family: gentianaceae) by reversed-phase preparative hplc coupled with a photo-diode-array detector. the structures of these compounds were elucidated unambiguously by uv, fabms and extensive 1d and 2d nmr spectroscopic analyses and also by comparing experimental data with literature data. antiba ...200312809366
pharmacological profile of extracts of pelargonium sidoides and their constituents.in areas of southern africa, aqueous extracts from the roots of pelargonium sidoides are employed to cure various disorders. nowadays, a modern formulation (eps 7630), elaborated from the traditional herbal medicine, is successfully used for the treatment of respiratory tract diseases. we previously observed that root extracts of p. sidoides and their representative constituents exhibit moderate antibacterial and significant immunomodulatory capabilities. the present study was performed to furth ...200312807338
extended-spectrum beta-lactamase producing enterobacteriaceae in a tertiary care hospital in trinidad and tobago.extended-spectrum beta-lactamase (esbl) mediated resistance to third generation cephalosporins, amongst the family enterobacteriaceae, is emerging worldwide. this is the caribbean's first survey on esbl production, and was conducted during two six-month periods in 1998 and 2001, in a tertiary health institution in trinidad and tobago. consecutive ampicillin resistant isolates of the family enterobacteriaceae from in-patients were screened for resistance to third generation cephalosporins, and fo ...200312806752
structure of the o-polysaccharide of proteus vulgaris o44: a new o-antigen that contains an amide of d-glucuronic acid with l-alanine.the o-polysaccharide of proteus vulgaris o44, strain prk 67/57 was studied by 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, roesy, h-detected 1h, 13c hmqc, hmqc-tocsy and hmbc experiments. the polysaccharide was found to contain an amide of d-glucuronic acid with l-alanine [d-glca6(l-ala)], and the following structure of the linear pentasaccharide repeating unit was established: [structure: see text]. the structural data of the o-polysaccharide and the results of serological studies wit ...200312801717
structure of the o-polysaccharide of providencia alcalifaciens o21 containing 3-formamido-3,6-dideoxy-d-galactose.the o-polysaccharide (o-antigen) of providencia alcalifaciens o21 was obtained by mild acid degradation of the lipopolysaccharide and studied by chemical methods and nmr spectroscopy. it was found that the polysaccharide is built up of branched pentasaccharide repeating units with a terminal residue of 3-formamido-3,6-dideoxy-d-galactose (d-fuc3nfo) and has the following structure: [structure: see text]. anti-p. alcalifaciens o21 serum cross-reacted with the o-antigen of proteus vulgaris o47, wh ...200312801716
crystal structure of a cyclic enterobacterial common antigen. 200312800183
mycotic aneurysm of the internal carotid artery--a case report.extracranial internal carotid artery (ica) mycotic aneurysms are rare entities that may result in significant neurologic morbidity and mortality. several operative techniques have been described in the literature for the management of this difficult condition. this case report describes a contained ruptured mycotic aneurysm of the extracranial ica associated with proteus mirabilis infection successfully treated by an end-to-end spatulated interposition saphenous vein graft.200312799731
chemical composition and antibacterial activity of essential oils from thymus spinulosus ten. (lamiaceae).the chemical composition of essential oils from aerial parts of thymus spinulosus ten. (lamiaceae) is reported. four oils from plants growing in different environmental conditions were characterized by gc and gc-ms methods; the oils seem to indicate a new chemotype in the genus thymus. influences of soil and altitude characteristics on the essential oil composition are discussed. the oils showed antibacterial activity against gram-positive (staphylococcus aureus, streptococcus faecalis, bacillus ...200312797754
comparative bactericidal activity of fluoroquinolones against clinical isolates resistant to fluoroquinolones.the bactericidal activity of levofloxacin, ciprofloxacin, moxifloxacin and norfloxacin against clinical isolates conventionally classified as resistant to fluoroquinolones were compared at their maximum concentrations in serum, urine (except moxifloxacin) and bronchial mucosa (except norfloxacin). time killing curves against escherichia coli, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, staphylococcus epidermidis, and streptococcus pneumoniae were performed. serum concentrat ...200312797386
antimicrobial resistance in gram-negative bacteria from intensive care units and urology services. a nationwide study in the netherlands 1995-2000.a nationwide 6-year surveillance of resistance in escherichia coli, klebsiella pneumoniae, proteus mirabilis and pseudomonas aeruginosa from clinical specimens of patients from ten intensive care units and ten urology services was started in 1995. mics of amoxycillin, amoxycillin/clavulanate, trimethoprim, cotrimoxazole, norfloxacin, ciprofloxacin, cefaclor, ceftazidime, imipenem and gentamicin were determined by broth microdilution. intensive care units had higher resistance levels of amoxycill ...200312791468
bartonella quintana lipopolysaccharide effects on leukocytes, cxc chemokines and apoptosis: a study on the human whole blood and a rat model.bartonella quintana, an emerging gram-negative pathogen, may cause trench fever, endocarditis, cerebral abscess and bacillary angiomatosis usually with the absence of septic shock in humans. b. quintana lipopolysaccharide (lps), a deep rough endotoxin with strong reactivity in the limulus amebocyte lysate (lal)-assay, was studied in human whole blood and in a rat model. a significant (p<0.05) increase of interleukin-8 (il-8) concentration, comparable to the level induced by enterobacterial lps, ...200312781702
characterization of escherichia coli strains causing urinary tract infections in patients with transposed intestinal segments.transposition of intestinal segments into the urinary tract predisposes to urinary tract infections. we characterized bacterial infections in these patients and examined the virulence genotype and persistence of escherichia coli isolates.200312771802
a survey of common pathogens in wound in patients at the university of port harcourt teaching hospital (u.p.t.h), port harcourt.during a 30-month period, january 1996 to june 1998, a total of 2458 wound swab samples were collected, cultured and identified. in descending order of frequency, the organisms isolated include: staphylococcus aureus (31.60%), escherichia coli (25.97%), pseudomonas aeruginosa (21.21%), klebsiella species (10.82%), proteus species (8.23%), non-haemolytic streptococci (1.29%), beta-haemolytic streptococcus (0.43%) and candida albicans (0.43%). the variation in frequency of the isolates according t ...200312769308
aetiological agents, clinical features and outcome of septicaemia in infants in ibadan.the present study sought to determine the prevalence, clinical features and bacterial aetiological agents of septicaemia in post-neonatal infants (age 1-12 months) presenting with fever at the university college hospital, ibadan, nigeria.200312769303
[an observation on long-term influence of middle ear bacterial infection on inner ear function and systemic immune reaction].to understand whether long-term inner ear heat shock response related to heat shock protein(hsp70) caused by middle ear bacterial infection and the potential influence on inner ear function.200012768776
new structures of the o-specific polysaccharides of proteus. 3. polysaccharides containing non-carbohydrate organic acids.four new proteus o-specific polysaccharides were isolated by mild acid degradation from the lipopolysaccharides of p. penneri 28 (1), p. vulgaris o44 (2), p. mirabilis g1 (o3) (3), and p. myxofaciens (4), and their structures were elucidated using nmr spectroscopy and chemical methods. they were found to contain non-carbohydrate organic acids, including ether-linked lactic acid and amide-linked amino acids, and the following structures of the repeating units were established: [figure: see text], ...200312765528
[long-term cefadroxil prophylaxis in children with recurrent urinary tract infections].the aim of the study was to evaluate the efficacy and safety of low-dose, long-term cefadroxil prophylaxis in preventing recurrent urinary tract infections in children. a prospective, randomized trial in 33 children (32 female, 1 male) aged 2-14 years (mean 8.1+/-2.8) was conducted. children with recurrent urinary tract infections were commenced to six-month prophylaxis with cefadroxil at the dosage of 12.5-15.0 mg/kg at bedtime administered every night (group i, n=15) or alternate night (group ...200312761422
visualization of proteus mirabilis morphotypes in the urinary tract: the elongated swarmer cell is rarely observed in ascending urinary tract infection.proteus mirabilis, a common cause of nosocomial and catheter-associated urinary tract infection, colonizes the bladder and ascends the ureters to the proximal tubules of the kidneys, leading to the development of acute pyelonephritis. p. mirabilis is capable of swarming, a form of multicellular behavior in which bacteria differentiate from the short rod typical of members of the family enterobacteriaceae, termed the swimmer cell, into hyperflagellated elongated bacteria capable of rapid and coor ...200312761147
structural and functional consequences of cleavage of human secretory and human serum immunoglobulin a1 by proteinases from proteus mirabilis and neisseria meningitidis.the cleavage of human serum monomeric immunoglobulin a1 (iga1) and human secretory iga1 (s-iga1) by iga1 proteinase of neisseria meningitidis and cleavage by the proteinase from proteus mirabilis have been compared. for serum iga1, both proteinases cleaved only the alpha chain. n. meningitidis proteinase cleaved only in the hinge. p. mirabilis proteinase sequentially removed the tailpiece, the ch3 domain, and the ch2 domain. the cleavage of s-iga1 by n. meningitidis proteinase occurred only in t ...200312761118
information extraction for enhanced access to disease outbreak reports.document search is generally based on individual terms in the document. however, for collections within limited domains it is possible to provide more powerful access tools. this paper describes a system designed for collections of reports of infectious disease outbreaks. the system, proteus-bio, automatically creates a table of outbreaks, with each table entry linked to the document describing that outbreak; this makes it possible to use database operations such as selection and sorting to find ...200212755518
[community acquired urinary tract infection among hospitalized children in northern israel: pathogens, susceptibility patterns and urinary tract anomalies].urinary tract infection (uti) is one of the most common diseases in children. vesicoureteral reflux (vur) has been demonstrated in a substantial number of young children with uti. empiric antibacterial therapy is recommended before results of the urine culture are available in order to shorten the duration of the disease and prevent renal complications.200312754871
isolation and characterization of phorate degrading soil bacteria of environmental and agronomic significance.phorate [o,o-diethyl-s-(ethylthio)methyl phosphoradiothioate] degrading bacteria were isolated from agricultural soil and characterized based on their morphological and biochemical characteristics. the selected isolates ps-1, ps-2 and ps-3 were presumptively identified as rhizobium, pseudomonas and proteous species, respectively. the hplc analysis of phorate in bioaugmented soil revealed its complete disappearance within 40 days. the degradation isotherms of the isolates ps-1, ps-2 and ps-3 sugg ...200312753240
production of dyestuffs from indole derivatives by naphthalene dioxygenase and toluene dioxygenase.to isolate and characterize the phorate [o,o-diethyl-s-(ethylthio)methyl phosphoradiothioate] degrading bacteria from agricultural soil, and their assessment for multifarious biological activities of environmental and agronomic significance.200312753239
[etiology and management of persistent hematospermia: a pilot study].to study the etiology of persistent hematospermia and to evaluate the efficacy of transrectal ultrasonography (trus)-guided transperineal needle aspiration and irrigation for diagnosis and treatment of persistent hematospermia.200312749132
crystallization of urine mineral components may depend on the chemical nature of proteus endotoxin polysaccharides.formation of infectious urinary calculi is the most common complication accompanying urinary tract infections by members of the genus proteus. the major factor involved in stone formation is the urease produced by these bacteria, which causes local supersaturation and crystallization of magnesium and calcium phosphates as carbonate apatite [ca(10)(po(4))(6).co(3)] and struvite (mgnh(4)po(4).6h(2)o), respectively. this effect may also be enhanced by bacterial polysaccharides. macromolecules of su ...200312748265
structural and serological studies of the o-antigen of proteus mirabilis o-9.the following structure of the o-polysaccharide (o-antigen) of the lipopolysaccharide of proteus mirabilis o-9 was determined by nmr spectroscopy, including 2d 1h,(1)h cosy, tocsy, roesy, and 1h,(13)c hmqc experiments, along with chemical methods: [chemical structure: see text] where the degree of o-acetylation is approximately 70%. immunochemical studies using rabbit polyclonal anti-proteus mirabilis o-9 serum showed the importance of the o-acetyl groups in manifesting the serological specifici ...200312747861
is combination antimicrobial therapy required for urinary tract infection in children?this retrospective study examined the characteristics of 338 pediatric patients presenting with a first episode of symptomatic urinary tract infection at taichung veterans general hospital from november 1996 to december 2001. escherichia coli was the most common pathogen (72.5%), followed by proteus mirabilis (8.3%), enterococcus (5.6%), and klebsiella pneumoniae (4.7%). they were more susceptible to first-generation cephalosporin in comparison with other first-line antimicrobial agents such as ...200312741735
management of adult facial vascular anomalies.vascular anomalies are best classified by biologic behavior-active hemangiomas, and inactive vascular malformations. hemangioma growth is restricted to infancy, but residual deformity may persist to adult life in the form of redundant skin, bulky fibrofatty tissue, and ectatic cutaneous vessels. treatment largely consists of scar revision and laser photocoagulation of residual vascular pigmentation. vascular malformations are present throughout life and can cause increasing facial distortion and ...200312739187
cytotoxicity responses to peptide antigens in rheumatoid arthritis and ankylosing spondylitis.to measure levels of igg antibodies against structurally related synthetic peptides of hla-drb1*0404, type xi collagen, and proteus mirabilis in patients with rheumatoid arthritis (ra) and hla-b*2705 and klebsiella pneumoniae in patients with ankylosing spondylitis (as), and to determine whether sera from ra and as patients are cytotoxic for sheep red blood cells (srbc) coated with hla-drb1*0404, type xi collagen, or hla-b*2705.200312734891
microbiologic profile of intra-abdominal infections at belo horizonte, brazil.intra-abdominal infections (iais) represent one of the most common clinical problems in hospital practice, especially in surgical areas and centers of intensive care. the treatment of iais generally involves the draining of abscesses and empirical antimicrobial therapy. in this study, among 150 patients suffering from iai, 106 (70.7%) yielded samples that presented microbial growth. polyinfection was detected in 51.9% of the cases and varied from 2 to 9 distinct microbes per specimen. the overal ...200312734518
[cefepime (maxipime), large spectrum 4th generation cephalosporin, resistant to beta-lactamases].as a result of the appearance of bacterial strains resistant to 3rd generation cephlosporin, since 1993 cephalosporins of 4th generation have been developed and introduced in therapy; among them are: cefepime and cefpirome. cefepime is the most active 4th generation cephalosporin possessing the following advantages over the 3rd generation cephalosporins: high intrinsic potency due to rapid penetration into the periplasmic space; an extended spectrum of activity that includes many gram-positive a ...200112731231
[cefepime (maxipime) treatment efficacy in surgical patients ].between september and december 2001 in the "caritas" surgical clinic of bucharest has been conducted a clinical study for the efficiency of cefepime (maxipime) treatment in surgical patients. introduced in therapy in the last decade of the xxth century, cefepime (maxipime) is the most active 4th generation cephalosporin, due to its extended spectrum of activity and its high resistance against beta-lactamases. cefepime (maxipime) has a very large spectrum, including the majority of the microorgan ...200212731215
susceptibility patterns of orally administered antimicrobials among urinary tract infection pathogens from hospitalized patients in north america: comparison report to europe and latin america. results from the sentry antimicrobial surveillance program (2000).urinary tract infections (utis) remain a worldwide nosocomial infection problem. geographic variations in pathogen occurrence and susceptibility profiles require monitoring to provide information to guide new (garenoxacin [bms284756]) therapeutic options. two thousand seven hundred-eighty uti isolates from europe (n = 783), latin america (531), and north america (1,466) were tested and compared against 44 agents by reference methods in the sentry antimicrobial surveillance program. the top seven ...200312730002
ruptured renal microaneurysms complicated with a retroperitoneal abscess for a patient with systemic lupus erythematosus.renal artery aneurysm is extremely rare among patients with systemic lupus erythematosus.(sle). herein, we report on a 22-year-old male lupus patient who presented with acute abdominal pain, anemia and subsequent hypertension. abdominal computed tomography revealed a peri-renal hematoma over the right kidney. a renal angiography revealed bilateral renal microaneurysms. the patient subsequently developed a right-side retroperitoneal abscess 4 weeks after hematoma formation and received an emergen ...200312729057
control of encrustation and blockage of foley catheters.urinary catheters often become encrusted and blocked by crystalline proteus mirabilis biofilms. results of experiments in a laboratory model of a foley catheterised bladder infected with p mirabilis showed that when retention balloons were inflated with a solution of triclosan (10 g/l), the catheters drained freely for at least 7 days. triclosan became impregnated throughout the silicone catheter material and completely inhibited the formation of crystalline biofilm, whereas catheters inflated w ...200312727400
evaluation of proteus mirabilis structural fimbrial proteins as antigens against urinary tract infections.proteus mirabilis is a common cause of urinary tract infection (uti) and produce several types of different fimbriae, including mannose-resistant/proteus-like fimbriae, uroepithelial cell adhesin (uca), and p. mirabilis fimbriae (pmf). different authors have related these fimbriae with different aspects of p. mirabilis pathogenesis, although the precise role of fimbriae in uti has not yet been elucidated. in this work we expressed and purified recombinant structural fimbrial proteins of these fi ...200312727373
comparative in vitro potency of amoxycillin-clavulanic acid and four oral agents against recent north american clinical isolates from a global surveillance study.the in vitro activity of amoxycillin-clavulanic acid was compared with four comparator oral antimicrobial agents; ampicillin, azithromycin, cefuroxime and trimethoprim-sulphamethoxazole against 4536 recent clinical isolates covering 29 species isolated in the us and canada between 1997 and 1999. based upon minimum inhibitory concentrations (mics), amoxycillin-clavulanic acid was the most active agent against many gram-positive species and phenotypes including methicillin susceptible staphylococc ...200312727075
an inducible tellurite-resistance operon in proteus mirabilis.tellurite resistance (te(r)) is widespread in nature and it is shown here that the natural resistance of proteus mirabilis to tellurite is due to a chromosomally located orthologue of plasmid-borne ter genes found in enteric bacteria. the p. mirabilis ter locus (terzabcde) was identified in a screen of tn5lacz-generated mutants of which one contained an insertion in terc. the p. mirabilis terc mutant displayed increased susceptibility to tellurite (te(s)) and complementation with terc carried on ...200312724390
[proteus syndrome: report of two cases].proteus syndrome is a complex disorder, consisting of mesodermal and skeletal malformations as well as hamartomatous overgrowth. it is characterized by highly variable presentation and mosaic-pattern lesions. the two cases described herein illustrate the wide polymorphism of this syndrome. the second case also had a pubic fracture, which we have not previously seen reported in the medical literature on proteus syndrome.200312724087
[enterobacter, citrobacter, proteus]. 200312722249
in vitro antimicrobial efficacy of leaf essential oils of chukrasia tabularis adr juss and melia dubia cav (meliaceae).the essential oils of chukrasia tabularis and melia dubia were tested for their antimicrobial activity against ten different pathogenic microorganisms responsible for human pathologies using standard antimicrobial assays. chukrasia tabularis leaf oil exhibited strong antibiotic activities against proteus vulgaris and fusarium oxysporum and did not show any activity against the tested bacteria. melia dubia leaf essential oil exhibited bacteriostatic and fungistatic activities against pseudomonas ...200312722153
[pyomyositis].pyomyositis is a bacterial infection of skeletal muscle. it is a rare condition which cannot easily be diagnosed. a fatal case of pyomyositis in a 78-year-old man is presented. the clinical signs and diagnostics of pyomyositis are discussed.200312715662
in vitro synergy studies using aztreonam and fluoroquinolone combinations against six species of gram-negative bacilli.combination antimicrobial therapy is often necessary to eradicate infections caused by gram-negative bacteria.200312714810
systematics of primary osmotrophic euglenids: a molecular approach to the phylogeny of distigma and astasia (euglenozoa).nuclear-encoded ssu rrna genes from nine strains of distigma and three strains of astasia were sequenced and analysed phylogenetically with maximum-likelihood and maximum-parsimony methods. it could be demonstrated that the genus distigma is paraphyletic, consisting of two distinct clades: one comprises four strains of the type species, distigma proteus, and the other includes four strains of distigma curvatum, distigma gracile, distigma sennii and distigma elegans. these findings are well corro ...200312710635
trends in antimicrobial susceptibilities among enterobacteriaceae isolated from hospitalized patients in the united states from 1998 to 2001.longitudinal surveillance of enterobacteriaceae for antimicrobial susceptibility is important because species of this family are among the most significant and prevalent human pathogens. to estimate rates of in vitro antimicrobial susceptibility among hospitalized patients in the united states, data from the surveillance network were studied for 14 agents tested against 10 species of enterobacteriaceae (n = 384,279) isolated from intensive-care-unit (icu) patients and non-icu inpatients from 199 ...200312709339
crystal structure of proteus vulgaris chondroitin sulfate abc lyase i at 1.9a resolution.chondroitin sulfate abc lyase i from proteus vulgaris is an endolytic, broad-specificity glycosaminoglycan lyase, which degrades chondroitin, chondroitin-4-sulfate, dermatan sulfate, chondroitin-6-sulfate, and hyaluronan by beta-elimination of 1,4-hexosaminidic bond to unsaturated disaccharides and tetrasaccharides. its structure revealed three domains. the n-terminal domain has a fold similar to that of carbohydrate-binding domains of xylanases and some lectins, the middle and c-terminal domain ...200312706721
bacteriology of burns at the queen elizabeth central hospital, blantyre, malawi.a retrospective study to determine the bacterial profile and antibiotic susceptibility pattern of burn isolates at the queen elizabeth central hospital (qech), blantyre, malawi was undertaken. while a total of 1825 burn patients were admitted to the hospital over a 6-year period (january 1994 to december 1999) only 317 (17.4%) specimens selected from as many patients were processed. one hundred and seventy (170/53.6%) specimens yielded single while the rest (147/46.4%) had multiple isolates givi ...200312706616
membrane fluidity sensoring microbial fuel cell.a study has been performed to examine the effect of temperature and ethanolic stresses on the coulombic efficiency of a microbial fuel cell. the conventional-type fuel cell containing gram-negative bacteria, proteus vulgaris, was investigated as a model system. from current output measurements, it was found that the coulombic yields were altered by environmental stresses such as temperature shock or ethanol treatment to the bacteria. while high-temperature or ethanolic shock led to a remarkable ...200312699828
effects of mupirocin at subinhibitory concentrations on flagella formation in pseudomonas aeruginosa and proteus mirabilis.colonization of pseudomonas aeruginosa at trachea, nares and oropharynx can cause ventilator-acquired pneumonia. to identify beneficial effects of antibiotics on expression of virulence factors related to colonization by such pathogens, we evaluated the effect of mupirocin on flagella formation in p. aeruginosa and on motility and flagella formation in proteus mirabilis. in p. aeruginosa, subinhibitory concentrations of mupirocin inhibited flagella formation, which was associated with reduced fl ...200312697640
a minimal form of proteus syndrome presenting with macrodactyly and hand hyperplasia.proteus syndrome is a rare congenital disorder characterized by progressive course and great variability of clinical presentation with partial gigantism of extremities, hemihyperplasia with macrocephaly, epidermal nevus, mesodermal hamartomas and the presence of peculiar cerebriform masses on the palms/soles. many atypical cases have been reported and this is probably due to the mosaicism of the genetic disorder displaying different clinical features. we describe a patient with an extremely mild ...200312695139
bacteraemia due to tribe proteeae: a review of 132 cases during a decade (1991-2000).to characterize the clinical features of bacteraemia due to tribe proteeae, 132 cases among 130 patients from 1991 to 2000 were analysed. the organisms included the proteus species in 63 cases (p. mirabilis in 41, p. penneri in 2 and p. vulgaris in 20), the providencia species in 8 (p. rettgeri in 3 and p. stuartii 5) and morganella morganii in 61. morganella bacteraemia occurred more frequently in the hospital (70.5%). biliary and hepatic diseases were predominant in cases with morganella bacte ...200312693558
immunochemical studies on the o-antigens of proteus mirabilis o23 and proteus vulgaris o23.analysis by 1h and 13c nuclear magnetic resonance (nmr) spectroscopy demonstrated that the o-specific polysaccharides of proteus mirabilis prk 42/57 and p. vulgaris prk 43/57 are structurally similar to that of p. vulgaris prk 44/57 and different from the polysaccharide of p. mirabilis prk 41/57 studied earlier. the lipopolysaccharides of these strains were tested using enzyme immunosorbent assay, passive hemolysis and western blot with o-antisera against p. mirabilis 42/57 and p. vulgaris 43/57 ...200312691306
representation, dispersion, and variation of bacterial indicators in the coastal waters of nagore (east coast of india).the occurrence of bacterial indicators in the coastal waters of nagore on the east coast of india was studied during two monsoon and nonmonsoon cycles from 1995 to1996. proteus/klebsiella, vibrio parahaemolyticus, and total coliforms were typically present at a mean level of 10(9) colony-forming units/g dry sediment, whereas other indicators such as salmonella, shigella, and v. cholerae were at the 10(5) level. in the water column, total coliforms and salmonella were 2 to 3 orders of magnitude l ...200312683465
tandem tetramer-based microsatellite fingerprinting for typing of proteus mirabilis strains.two microsatellite tandem repeated tetramers, (gaca)(4) and (caat)(4), were used for proteus mirabilis strain differentiation. the microsatellite-based pcr tests were applied for the examination of interstrain diversity for 87 p. mirabilis strains. forty-six of the investigated strains were clinical isolates (5 were hospital isolates and 39 were outpatient clinic isolates); 42 strains were derived from the kauffmann-perch collection of laboratory strains. fingerprinting done with the tetramers h ...200312682159
characterization of clinical isolates of enterobacteriaceae from italy by the bd phoenix extended-spectrum beta-lactamase detection method.production of extended-spectrum beta-lactamases (esbls) is an important mechanism of beta-lactam resistance in enterobacteriaceae: identification of esbls based on phenotypic tests is the strategy most commonly used in clinical microbiology laboratories. the phoenix esbl test (bd diagnostic systems, sparks, md.) is a recently developed automated system for detection of esbl-producing gram-negative bacteria. an algorithm based on phenotypic responses to a panel of cephalosporins (ceftazidime plus ...200312682131
structure of the o-specific polysaccharide of providencia rustigianii o14 containing n(epsilon)-[(s)-1-carboxyethyl]-n(alpha)-(d-galacturonoyl)-l-lysine.the o-specific polysaccharide of providencia rustigianii o14 was obtained by mild acid degradation of the lps and studied by chemical methods and nmr spectroscopy, including 2d 1h,(1)h cosy, tocsy, noesy, and 1h,(13)c hsqc experiments. the polysaccharide was found to contain n (epsilon)-[(s)-1-carboxyethyl]-n(alpha)-(d-galacturonoyl)-l-lysine ('alaninolysine', 2s,8s-alalys). the amino acid component was isolated by acid hydrolysis and identified by 13c nmr spectroscopy and specific optical rotat ...200312681927
treatment of gram-negative folliculitis in patients with acne.gram-negative folliculitis may be the result of long-term antibacterial treatment in acne patients. it is caused by bacterial interference and replacement of the gram-positive flora of the facial skin and the mucous membranes of the nose and infestation with gram-negative bacteria. these gram-negative bacteria include escherischia coli, pseudomonas aeruginosa, serratia marescens, klebsiella and proteus mirabilis. the occurrence of gram-negative folliculitis should be considered in acne patients ...200312680804
[tuberculous otitis media. report of 3 cases].tuberculous otitis media (tom) is a rare cause of chronic suppurative infection of the middle ear. due to that the symptoms and signs are often indistinguishable from those of nontuberculosis chronic otitis media and the fact that the index of suspicion is low, there is frequently a considerable delay prior to diagnosis. this can lead to irreversible complications such as facial nerve paralysis and labyrinthitis. medical therapy with antituberculous drugs is usually effective. we report three ca ...200312680299
aerobic pathogenic bacteria in post-operative wounds at moi teaching and referral hospital.the emergence of bacterial antimicrobial resistance associated with acquired infections has made the choice of empirical therapy more difficult and expensive, hence the need for continuous research to determine their sensitivity patterns.200212678447
natural antibiotic susceptibility of proteus spp., with special reference to p. mirabilis and p. penneri strains.the natural susceptibility of 102 proteus mirabilis and 35 proteus penneri strains to 71 antibiotics was examined. minimum inhibitory concentrations (mics) were determined by applying a microdilution procedure in isosensitest broth (for all strains) and cation-adjusted mueller hinton broth (for some strains). p. mirabilis and p. penneri were naturally resistant to penicillin g, oxacillin, all tested macrolides, lincosamides, streptogramins, glycopeptides, rifampicin and fusidic acid. both specie ...200312678409
molecular analysis of the microflora in chronic venous leg ulceration.there is growing evidence to suggest that the resident microflora of chronic venous leg ulcers impairs cellular wound-healing responses, thereby playing an important role in maintaining the non-healing phenotype of many of these wounds. the significance of individual species of bacteria will remain unclear until it is possible to characterize fully the microflora of such lesions. the limitations and biases of culture-based microbiology are being realized and the subsequent application of molecul ...200312676877
involvement of nitric oxide donor compounds in the bactericidal activity of human neutrophils in vitro.the bactericidal activity of human neutrophils against extracellular and facultatively intracellular bacteria was studied in the presence of the nitric oxide (no) donors sodium nitroprusside (snp) and 3-morpholinosydnonimine (sin-1), a molsidomine metabolite. snp and molsidomine are drugs commonly used as nitrovasodilators in coronary heart disease. it is demonstrated here that the no donor compounds themselves did not affect the viability and survival of the bacterial strains tested. neither sn ...200312676868
extended spectrum beta-lactamase mediated resistance in urinary tract isolates of family enterobacteriaceae.extended spectrum beta-lactamases (esbl) hydrolyse expanded spectrum cephalosporins like ceftazidime, cefotaxime and monobactam aztreonam. esbl producing bacteria may not be detectable in the routine disk diffusion susceptibility tests leading to inappropriate use of antibiotics and treatment failures. as information on esbl producers causing urinary tract infection (uti) is not available from our country, the occurrence of esbl producing strains causing uti was studied as also the differences b ...200212674828
characterization of sams genes of amoeba proteus and the endosymbiotic x-bacteria.as a result of harboring obligatory bacterial endosymbionts, the xd strain of amoeba proteus no longer produces its own s-adenosylmethionine synthetase (sams). when symbiont-free d amoebae are infected with symbionts (x-bacteria), the amount of amoeba sams decreases to a negligible level within four weeks, but about 47% of the sams activity, which apparently comes from another source, is still detected. complete nucleotide sequences of sams genes of d and xd amoebae are presented and show that t ...200312674481
antibiotic resistance patterns of uropathogens in pediatric emergency department patients.to evaluate the prevalence of resistance of the various urinary tract infection (uti) pathogens obtained from patients in an urban pediatric emergency department (ped), and to identify risk factors for infection with resistant strains.200312670848
proteus mirabilis glutathione s-transferase b1-1 is involved in protective mechanisms against oxidative and chemical stresses.we investigated the effects of several xenobiotics, including antimicrobial agents and general stress factors such as starvation, heat and osmotic shock, on the modulation of expression of proteus mirabilis glutathione s-transferase b1-1 (pmgst b1-1). the level of expression of pmgst b1-1 was established by both western- and northern-blot experiments. our results show that several compounds can modulate expression of pmgst b1-1. the level of pmgst b1-1 increased when bacterial cells were exposed ...200312667139
synthesis and antimicrobial activity of [2-[2-(n, n-disubstituted thiocarbamoyl-sulfanyl)-acylamino] thiazol-4-yl]acetic acid ethyl esters.[2-[2-(n, n-disubstituted thiocarbamoyl-sulfanyl)acylamino ]thiazol-4-yl]acetic acid ethyl esters (3a-x) were synthesized by the reaction of potassium salts of n, n-disubstituted dithiocarbamoic acids with [2-(2-chloroalkanoyl)amino-thiazol-4-yl]acetic acid ethyl esters. the structures of the synthesized compounds were confirmed by elemental analyses, uv, ir, (1)h-nmr, and ei mass spectral data. the antimicrobial activities of all the compounds were investigated by microbroth dilution technique ...200312666252
[morganella morganii endophthalmitis after vitrectomy: case report and review of the literature].postoperative bacterial endophthalmitis are caused in 80 % of the cases by the patient's own flora. most of the time, bacterial agents are gram-positive ((2/3) of cases) and more rarely gram-negative ((1/3) of cases). usually, pseudomonas sp, proteus sp or klebsiella sp are isolated, but very rarely morganella morganii.200312664382
effect of rho family gtp-binding proteins on amoeba proteus.while there is a number of studies on the effects of rho gtpases on the actin-based cytoskeleton in higher eukaryotes, studies in protozoans are rather limited. the problem seems to be intriguing since the structure of protozoan cytoskeletons is distinct from most vertebrate cells. by blocking endogenous rho family proteins of highly motile amoeba proteus with c3 transferase and antibodies against human rhoa and rac1, we tried to assess the in vivo role of these proteins in amoebae. in migrating ...200312664280
what have we learnt from community-acquired infections in hong kong?this study was initiated throughout hong kong, to reveal the characteristics of community-acquired infections. all specimens collected by general practitioners from infected patients were followed prospectively, and those that were culture-positive were analysed. four thousand seven hundred and forty-one specimens were collected from 3977 patients by 89 doctors from july 2000 to october 2001. the most common specimens were throat swabs (33%), urine (26%) and sputa (16%). the average culture-posi ...200312654732
[investigation of hydrophobicity of proteus vulgaris strains and ability of proteus vulgaris and proteus penneri strains to penetrate bladder membrane hcv t-29 cells ].proteus bacilli play a particularly important role in urinary tract infections (uti). fimbriae and adherence ability and hemolysins production (hpma, hlya) are one of the factors of pathogenicity of these bacteria. in this paper we describe the invasion of hcv t-29 transitional bladder urothelial cells carcinoma strains of p. penneri, as well as p. vulgaris strains belonging to different serogroups. the cytotoxic effect was observed at 8 hour of incubation of the tested cells with p. vulgaris o2 ...200212650056
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