| [physical and genetic organization of the plasmid r15]. | the conjugative incn plasmid r15 (smrsurhgr, 62.3 kb) is cleaved by the hexanucleotide-specific endonucleases bglii, hindiii, ecori, bamhi, smai, sali, psti and xhoi into 9, 9, 6, 5, 4, 4, 4 and 2 fragments, respectively. the restriction sites were located on the physical map of the r15 genome. distribution of the cleavage sites is strongly asymmetric. 28 of 32 sites for bamhi, ecori, hindiii, sali, smai and psti were located close to or within the sequences of transposable elements tn2353 and t ... | 1985 | 2991086 |
| [isolation and purification of restriction endonuclease pmii from proteus mirabilis 1667]. | a new restriction endonuclease pmi i was detected in proteus mirabilis 1667. the enzyme hydrolyzes dna of the phage lambda into 10 electrophoretically separating fragments with molecular weights of 1.3-7.9 md. with the use of two-stage chromatography on blue sepharose and phosphocellulose it is possible to obtain restriction endonuclease pmi i free of the admixtures of ballast proteins, nonspecific nucleases and phosphatases. | 1985 | 2992364 |
| a site-specific endonuclease from pseudomonas aeruginosa. | paei, a new restriction endonuclease from pseudomonas aeruginosa clinical strain was isolated and characterized. it recognizes and cleaves the sequence 5'-gcatg reduced c-3' generating dna fragments with 3'-tetranucleotide sticky ends. dnas of pbr322, sv40 and bacteriophage lambda have one, two and six paei recognition sites, respectively. seventy-two strains of pseudomonas, clostridium, escherichia coli, shigella, proteus and saccharomyces were screened for the presence of site-specific endonuc ... | 1985 | 2993850 |
| clinical trial of cefoxitin (mefoxin) in parenteral therapy of septicemia (postabortal and postpartum) in ibadan. | cases of post-abortal sepsis are admitted every day into the university college hospital (uch), ibadan, nigeria. this derives from the high incidence of induced (illegal) abortions in this environment. the infections are usually caused by mixed bacterial flora, often resistant to the common antibiotics because of the indiscriminate use of these drugs. any new drug that can be effective in the treatment of these resistant cases will be welcome. the efficacy of cefoxitin in the treatment of twenty ... | 1985 | 2994436 |
| chemical preparation of the eye in ophthalmic surgery. iv. comparison of povidone-iodine on the conjunctiva with a prophylactic antibiotic. | we previously found that half-strength (5%) povidone-iodine solution significantly reduced the bacterial flora of the conjunctiva. to compare the antibacterial effect of a topical combination antibiotic (neosporin ophthalmic solution) given three times daily for three days preoperatively with that of half-strength povidone-iodine solution given as part of the preoperative preparation, conjunctival cultures were studied from 35 patients undergoing ocular surgery. when used individually, the antib ... | 1985 | 2994609 |
| opsonized bacteria stimulate leukotriene synthesis in human leukocytes. | incubation of human leukocytes with opsonized bacteria led to leukotriene formation. the main products identified were leukotriene b4, 20-oh leukotriene b4 and 20-cooh leukotriene b4. a lesser amount of leukotriene c4 was formed. in contrast, only minor amounts of leukotrienes were formed by leukocytes challenged with uncoated bacteria. however, both opsonized and unopsonized bacteria stimulated the synthesis of 5s,12s-dhete and 5s,12s,20-thete. opsonized bacteria caused a transient elevation of ... | 1985 | 2994745 |
| cloning of a restriction-modification system from proteus vulgaris and its use in analyzing a methylase-sensitive phenotype in escherichia coli. | a 4.84-kilobase-pair plasmid was isolated from proteus vulgaris (atcc 13315) and cloned into the plasmid vector pbr322. plasmid pbr322 contains substrate sites for the restriction endonucleases pvui and pvuii. the recombinant plasmids were resistant to in vitro cleavage by pvuii but not pvui endonuclease and were found to cause production of pvuii endonuclease or methylase activity or both in escherichia coli hb101. the approximate endonuclease and methylase gene boundaries were determined throu ... | 1985 | 2997113 |
| [immune response as a function of the nutritional status in young children 1 to 3 years of age in the south of ivory coast]. | 177 children between 1 and 3 years (74 well-nourished, 55 suspected of protein/calorie deficiency, 48 under nourished) were vaccinated (tuberculosis, diphtheria, tetanus, whooping-cough, polio); one month after the third dose of dtwc polio, we proceeded to apply the merieux multitest and to check for intradermal reaction to the tuberculin: this study has shown that the merieux multitest gives results comparable to the classical intradermal method with tuberculin. the multitest makes it possible ... | 1985 | 2999558 |
| physical and genetic structure of the incn plasmid r15. | restriction sites for seven hexanucleotide-specific endonucleases were located on the map of the conjugative incn plasmid r15 (smrsurhgr, 62.3 kb). the distribution of the cleavage sites is strongly asymmetric. twenty-eight of thirty-four sites for bamhi, ecori, hindiii, sali, smai, and psti were located close to or within the sequences of an is5-like element and the transposons tn2353 and tn2354. by analysis of r15::tn1756 deletion derivatives and recombinant plasmids harboring r15 fragments, t ... | 1985 | 2999854 |
| evaluation of intracellular killing of bacteria by enriched populations of mouse peritoneal exudate neutrophils. | elicited, mouse peritoneal exudate cells were fractionated by centrifugation on discontinuous percoll density gradients. two subpopulations of neutrophils, each of greater than 90% purity, were isolated at discontinuous density gradient interfaces different from the region of mononuclear cell enrichment (i.e., 1.0694-1.0871 and 1.0872-1 x 1002 g/ml for neutrophils and less than 1.0694 g/ml for mononuclear cells). peritoneal exudate cells were mixed with proteus mirabilis in the presence of 1% no ... | 1985 | 3002310 |
| preliminary report on the in vitro antibacterial activity of bryophyllum pinnatum leaf juice. | the juice from the leaves of bryophyllum pinnatum s. kurtz (crassulaceae) was tested for antibacterial activity. the extract at 5% v/v was bactericidal to a wide spectrum of gram-positive and gram-negative bacteria such as bacillus subtilis, staphyllococcus aureus, streptococcus pyogenes; streptococcus faecalis; escherichia coli; proteus spp; klebsiella spp; shigella spp; salmonella spp; serratia marcescens; and pseudomonas aeruginosa including the clinical isolates of these organisms possessing ... | 1985 | 3004181 |
| protease production by microorganisms associated with reproductive tract infection. | factors influencing pathogenicity of various microbes found in the female lower genital tract remain incompletely understood. protease production by cervico/vaginal microorganisms may alter or inactivate a variety of proteins important in host defense and structural-functional integrity including collagen-containing chorioamniotic membranes and uterine cervix. host tissues may be made more susceptible to other organisms' virulence factors by protease-producing members of genital tract local flor ... | 1986 | 3004213 |
| [similar organization of beta,beta'-subunit rna-polymerase genes and adjacent ribosomal protein genes in enterobacteriaceae and pseudomonas putida]. | the genes coding for the rna-polymerase beta,beta'-subunits and adjacent ribosomal protein genes in escherichia coli, salmonella typhimurium, shigella flexneri, serratia marcescens, proteus mirabilis and pseudomonas putida are compared by the southern hybridization procedure. in all the species studied close clustering of the genes rplkajl and rpobc is demonstrated. preliminary physical maps for these genes in s. typhimurium, s. flexneri, s. marcescens and p. mirabilis are proposed. rifampicin i ... | 1986 | 3005845 |
| expression of the proteus mirabilis lipoprotein gene in escherichia coli. existence of tandem promoters. | the ipp gene from proteus mirabilis was cloned onto pbr322 and expressed in escherichia coli. the p. mirabilis lpp gene is unique in that it has two tandem promoters transcribing two mrnas that differ in length by approximately 70 nucleotides at their 5'-ends. the two mrnas thus encode the identical lipoprotein. the p. mirabilis prolipoprotein has a 19-amino acid signal peptide and a 59-amino acid lipoprotein sequence. in spite of the substantial differences in the amino acid sequence from the e ... | 1986 | 3007466 |
| peptides as modifiers of na+-induced pinocytosis in starved amoeba proteus. | low concentrations of six peptide hormones; glucagon, vasoactive intestinal peptide, substance p, angiotensin ii, lysine-vasopressin, arginine-vasopressin, and the chemotactic peptide fmet-leu-phe, activated the capacity for pinocytosis in starved amoeba proteus. competitive inhibitors of the chemotactic peptide in leucocytes inhibited activation by fmet-leu-phe, suggesting that its action in the amoeba is mediated by specific receptors. the opioid peptides, beta-endorphin, dynorphin (1-13) and ... | 1985 | 3008125 |
| susceptibility of gram-negative bacteria to the synergistic bactericidal action of serum and polymyxin b nonapeptide. | polymyxin b nonapeptide was able to sensitize escherichia coli strains and strains of salmonella typhimurium, klebsiella spp., enterobacter cloacae, pseudomonas aeruginosa, and haemophilus influenzae to the bactericidal action of fresh normal human serum. the degree of sensitization varied significantly within the strains. strains of proteus mirabilis, neisseria gonorrhoeae, and n. meningitidis remained resistant. | 1986 | 3008971 |
| [host defense mechanism in pyelonephritis--activation of peritoneal macrophage and its protective effect in the rat with retrograde proteus mirabilis pyelonephritis]. | | 1985 | 3012169 |
| susceptibility of aerobic gram-negative bacilli to aminoglycosides. effects of 45 months of amikacin as first-line aminoglycoside therapy. | amikacin was instituted as the primary empiric aminoglycoside at the san juan veterans administration medical center in january 1982; at that time, 16 percent of the strains at the hospital were gentamicin-resistant. a prospective surveillance study was designed to correlate detection of bacterial resistance with aminoglycoside use. in the current report, the baseline period, during which gentamicin was the first-line aminoglycoside, accounting for 61 percent of overall aminoglycoside use, is co ... | 1986 | 3014877 |
| comparative evaluation of a new beta-lactamase inhibitor, ytr 830, combined with different beta-lactam antibiotics against bacteria harboring known beta-lactamases. | ytr 830, a new beta-lactamase inhibitor, combined with amoxicillin or carbenicillin, showed a synergistic effect similar to that observed with clavulanic acid, and generally better than that with sulbactam, against strains harboring chromosome-encoded penicillinases and broad-spectrum beta-lactamases or plasmid-determined beta-lactamases. with ampicillin, ytr 830 showed the best synergistic activity of the inhibitors against proteus morganii, citrobacter freundii, and enterobacter cloacae and th ... | 1986 | 3015011 |
| interaction between pyridine adenine dinucleotides and bovine liver catalase: a chromatographic and spectral study. | two different fractions were present in crystalline bovine liver catalase, and could be resolved using dye-ligand affinity chromatography with red-a matrex gel containing procion he 3b. the major part (alpha) was not adsorbed on this gel. the second fraction (beta) was firmly adsorbed to the gel, and could be eluted either by high salt or by nadph in the micromolar range. elution of catalase beta was also obtained with nadh, nadp+, and adp at higher concentration. fractions alpha and beta displa ... | 1986 | 3015030 |
| potential prodrugs of 6-acetylmethylenepenicillanic acid (ro 15-1903). | the synthesis and biological activities of a series of non-classical penicillins are described. these compounds were synthesized by treating the pivaloyloxymethyl ester of 6-acetylmethylenepenicillanic acid (ro 15-1903) with various nucleophiles. they were found to be less active against the beta-lactamases from proteus vulgaris 1028, escherichia coli 1024, klebsiella pneumoniae nctc 418 and e. coli rtem than the parent compound. nevertheless, synergy with ampicillin against whole bacterial cell ... | 1986 | 3015853 |
| pyridoxal 5'-phosphate-dependent histidine decarboxylase. nucleotide sequence of the hdc gene and the corresponding amino acid sequence. | the nucleotide sequence of a 1.3-kilobase naei fragment from morganella morganii am-15 that contains the gene for histidine decarboxylase has been determined. the gene was initially identified among total chromosomal digests using a mixed sequence oligonucleotide probe corresponding to amino acids 11-16 of histidine decarboxylase and then cloned on a 5.5-kilobase psti fragment. the structural gene contains 1131 nucleotides and encodes 377 amino acids with the sequence: (sequence: in text). the i ... | 1986 | 3015950 |
| dissemination of a gentamicin resistance plasmid in the microbial population of hospital patients. | the dissemination of a gentamicin resistant plasmid, originally found in strains of klebsiella and termed pk181, into the microbial population of patients of the orvieto hospital was studied during 1982. five hundred and seventy-four strains of gram-negative bacilli were examined, transferable gentamicin resistance being revealed in five different bacterial species. the resistance was shown to be encoded by 81-megadalton plasmids in escherichia coli and enterobacter cloacae, and by 93-megadalton ... | 1986 | 3018453 |
| in vitro evaluation of sf-2103a, a novel carbapenem antibiotic, as a beta-lactamase inhibitor. | sf-2103a, a new carbapenem antibiotic, exhibited a broad antibacterial spectrum and a potent inhibitory activity against a wide range of beta-lactamases, in particular, against cephalosporinases, with lower i50 values than those displayed by sulbactam and clavulanic acid. using a fixed combination and checkerboard titration, in vitro synergy against the majority of the beta-lactamase-producing strains tested was demonstrated between sf-2103a and various beta-lactam antibiotics, especially cefota ... | 1986 | 3019978 |
| comparative activities of the beta-lactamase inhibitors ytr 830, clavulanate and sulbactam combined with extended-spectrum penicillins against ticarcillin-resistant enterobacteriaceae and pseudomonads. | the in-vitro synergistic activity of ytr 830, a new beta-lactamase inhibitor, combined with four extended-spectrum penicillins (ticarcillin, piperacillin, mezlocillin and apalcillin) against ticarcillin-resistant clinical isolates of gram-negative enteric bacilli was compared with that of clavulanate and sulbactam. synergy testing was performed with fixed concentrations of beta-lactamase inhibitors (8 mg/l) combined with doubling dilutions of beta-lactams in microdilution trays. synergy was defi ... | 1986 | 3019984 |
| synergy of amoxycillin combined with clavulanate and ytr 830 in experimental infections in mice. | ytr 830, a new beta-lactamase inhibitor, is synergistic with amoxycillin in vitro against a number of beta-lactamase-producing organisms. the combination of amoxycillin-ytr 830 was compared to amoxycillin-clavulanate in the treatment of experimental staphylococcus aureus, citrobacter freundii and proteus mirabilis infections in mice. both combinations were synergistic with amoxycillin against all three test organisms. the amoxycillin-clavulanate combination was superior against s. aureus and c. ... | 1986 | 3019987 |
| comparative study of some biological activities of porins extracted from various microorganisms. | the outer membrane of gram-negative bacteria contains some major proteins, called porins, with particular chemical and physical characteristics which reflect some specialized functions peculiar to the outer membrane. in recent years the biological properties of salmonella typhimurium sh5014 porins have been the focus of our experimental studies. the aim of the present research was to make a comparative investigation of the biological activities of porins extracted from other microorganisms class ... | 1986 | 3022112 |
| restriction enzyme fingerprinting of enterobacterial plasmids: a simple strategy with wide application. | restriction enzyme fingerprints were generated from purified plasmid dna from 324 clinical isolates that belonged to 7 enterobacterial genera and 88 single plasmids in escherichia coli k 12 according to the following strategy. purified plasmid dna was digested with psti. the number of fragments detected in a 0.8 agarose gel was used to determine which 2 of 6 restriction enzymes including psti was most likely to provide a fingerprint comprising sufficient fragments to ensure specificity but suffi ... | 1986 | 3023479 |
| sulbactam: biochemical factors involved in its synergy with ampicillin. | sulbactam is a time-dependent irreversible inhibitor of various beta-lactamases by reversible formation of a michaelis-type enzyme-inhibitor complex and progressive evolution of this complex into inactivated protein(s). this process is either irreversible (true inactivation) or quasi-irreversible (stable acyl-enzyme). in this way, sulbactam efficiently protects ampicillin from degradation by beta-lactamases. sulbactam itself exhibits a moderate antibacterial activity that is related to an affini ... | 1986 | 3025996 |
| sulbactam/ampicillin: in vitro spectrum, potency, and activity in models of acute infection. | more than 90% of community hospital-isolated strains of staphylococcus (including methicillin-resistant isolates), streptococcus, haemophilus, neisseria, branhamella, bacteroides, escherichia coli, klebsiella, enterobacter aerogenes, proteus, and acinetobacter calcoaceticus were inhibited by the sulbactam/ampicillin (1:2) combination at concentrations of 8 micrograms/16 micrograms per ml. the peak serum level from a 15-min infusion of 1 g/2 g of sulbactam/ampicillin is more than seven times this ... | 1986 | 3026001 |
| activity of sulbactam/ampicillin in screening and discriminative animal models of infection. | the efficacy of sulbactam/ampicillin in the treatment of mice with fatal systemic infections produced by ampicillin-resistant staphylococcus aureus, haemophilus influenzae, klebsiella pneumoniae, or proteus vulgaris strains is well established. in this paper the demonstrations of efficacy for sulbactam/ampicillin have been extended to a number of clinically relevant models, including bacteremia and meningitis produced by h. influenzae in infant rats, experimental staphylococcal endocarditis in r ... | 1986 | 3026002 |
| induction/inhibition of chromosomal beta-lactamases by beta-lactamase inhibitors. | many species of gram-negative bacteria produce chromosomal beta-lactamases that can be induced to high-level expression by exposure to beta-lactam antibiotics. fifty-four strains of escherichia coli, enterobacter, proteus, citrobacter, and morganella were examined for beta-lactamase inducibility by clavulanic acid, sulbactam, ytr 830, ampicillin, and cefoxitin. cefoxitin proved to be the most potent inducer, affecting most of the strains of enterobacter and morganella. clavulanic acid induced 30 ... | 1986 | 3026004 |
| urinary calculi and urinary tract infection. a clinical and microbiological study. | the problem of urinary calculi in association with urinary tract infection (uti) was investigated. fifty-two (7%) of 796 patients attending our outpatient stone clinic had uti which was considered of pathogenic importance for their stone formation. proteus was the most common microorganism. metabolic disorders were found in one third and anatomical and functional abnormalities in two thirds of the patients. the infected patients had lower urinary calcium excretion and higher serum creatinine tha ... | 1986 | 3026032 |
| [gyrase inhibitor in the local treatment of the chronically infected middle ear following surgery]. | local treatment of 20 chronic bacterial ear infections (18 infected radical cavities, 2 external otitis) with a gyrase inhibitor (ciprofloxacin) solution led to clinical resolution in 17 cases, with elimination of the causative organisms in 16 cases. no side effects were seen, but a resistant pseudomonas developed in one case, and candida albicans in 8 others. gyrase inhibitors such as ciprofloxacin are antibiotics of second choice. for local treatment in the ear they should be used only in diff ... | 1986 | 3028994 |
| lethal genes surviving by mosaicism: a possible explanation for sporadic birth defects involving the skin. | a genetic concept is advanced to explain the origin of several sporadic syndromes characterized by a mosaic distribution of skin defects. it is postulated that these disorders are due to the action of a lethal gene surviving by mosaicism. the presence of the mutation in the zygote will lead to death of the embryo at an early stage of development. cells bearing the mutation can survive only in a mosaic state, in close proximity with normal cells. the mosaic may arise either from a gametic half ch ... | 1987 | 3033033 |
| identification of porins in outer membrane of proteus, morganella, and providencia spp. and their role in outer membrane permeation of beta-lactams. | proteus mirabilis, proteus vulgaris, morganella morganii, providencia rettgeri, and providencia alcalifaciens, which were once classified into the same genus, proteus, were studied. cefoxitin-resistant mutants from these species were isolated, and it was confirmed that the resistance was attributed to the lack of an outer membrane protein, resulting in a significant decrease in the penetration of hydrophilic cephalosporins through the outer membrane. comparison of the mutant strains with their p ... | 1987 | 3034144 |
| in-vitro anti-microbial susceptibility of clinical isolates of pathogenic bacteria to ten antibiotics including phosphomycin. | the minimum inhibitory concentration (mic) of ten antibiotics was determined for various bacterial pathogens isolated from clinical specimens in a lagos hospital. the in-vitro activity of penicillin and tetracycline was not very impressive and a similarity was noticed in the resistance patterns of these two antibiotics, while the activity in vitro of the relatively more toxic aminoglycosides and chloramphenicol was high for gram-negative rods. ceftazidime demonstrated the highest activity in vit ... | 1987 | 3037870 |
| in vitro antimicrobial activity of diethyldithiocarbamate and dimethyldithiocarbamate against methicillin-resistant staphylococcus. | staphylococcus aureus has appeared which is highly resistant to both methicillin and aminoglycosides. current therapy involves long-term intravenous therapy of vancomycin. since vancomycin is currently the only drug used to treat these patients, there is a need to develop additional antimicrobial therapy. the in vitro antimicrobial effect of the metal chelator, diethyldithiocarbamate (ddtc) and its structural analog dimethyldithiocarbamate (dmtc) were investigated. both ddtc and dmtc were effect ... | 1987 | 3037985 |
| klebsiella antibodies in ankylosing spondylitis and proteus antibodies in rheumatoid arthritis. | antibodies to klebsiella, but not to other bacteria, have been shown to be present in patients with active ankylosing spondylitis (as) by seven different techniques. antibodies to proteus, but not to e. coli or klebsiella, have been shown to be present in patients with active rheumatoid arthritis (ra) by three different techniques. it is suggested as and ra are forms of reactive arthritis, to klebsiella and proteus respectively, probably mediated by cross-reactivity to hla antigens. | 1988 | 3042077 |
| antibodies to klebsiella and proteus microorganisms in ankylosing spondylitis and rheumatoid arthritis patients measured by elisa. | antibodies to klebsiella oxytoca and proteus mirabilis in 21 active ankylosing spondylitis (as), 13 active rheumatoid arthritis (ra), 19 inactive ra patients and 18 healthy controls were measured using enzyme-linked immunosorbent assay (elisa). elevated anti-klebsiella antibodies were demonstrated in active as patients compared to active ra (p less than 0.01), inactive ra patients (p less than 0.001) and controls (p less than 0.005). when measuring antibodies to proteus, active ra patients showe ... | 1988 | 3042078 |
| antibodies to proteus in rheumatoid arthritis. | increased levels of proteus antibodies were found in patients with rheumatoid arthritis and coeliac disease, when compared to normal controls or patients with sle and sarcoidosis. | 1988 | 3042079 |
| the dienes effect as an epidemiological tool in a paraplegic unit. | the authors study the dienes effect in 136 p.mirabilis and 2 p.vulgaris strains, isolated from different infection or colonization sites, in 27 spinal cord injured patients, admitted to a paraplegic unit, during a 9 month period. it is shown that 7 cross-infection and 18 cross-contamination episodes occurred, affecting 13 of 27 patients, which indicates the great spreading capacity of these microorganisms; 97% of the results obtained were observed once again after 6 months' storage of strains. i ... | 1988 | 3042449 |
| immunization with rough mutants of salmonella minnesota: initial studies in human subjects. | vaccines prepared from unheated and boiled, acetone-precipitated salmonella minnesota r595 (re chemotype mutant) were administered subcutaneously to 122 healthy volunteers. titers of antibody to re lipopolysaccharide, the basal core structure of endotoxin, as measured by indirect hemagglutination, rose in a dose-responsive fashion after immunization. febrile reactions, usually mild, occurred after 7% of injections with the highest doses (2.0 and 3.0 x 10(10) organisms), and mild local soreness a ... | 1988 | 3042872 |
| [in vitro bactericidal effect of cefotetan-aminoside combinations]. | bactericidal activity of cefotetan-gentamicin combinations was studied on 6 bacterial strains: s. aureus, k. pneumoniae, e. cloacae, s. marcescens, p. vulgaris, p. stuarti. time kille curves technic was performed with final concentrations of cefotetan: 4 at 32 mg/l, and of gentamicin: 0.25; 0.5; 1; 2; 8 mg/l and with an 10(6) cfu/ml inoculum. cefotetan at 4 mg/l was not able to obtain a 0.01% (percentage of survivors) bactericidal activity before 24 h. the combination cefotetan-gentamicin (0.25 ... | 1988 | 3043329 |
| [current status of aminoglycoside resistance in hospital enterobacteriaceae]. | we studied the susceptibility of enterobacteriaceae to four aminoglycosides (gentamicin, tobramycin, netilmicin et amikacin). we determined their phenotypes of resistance by taking into account both the critic concentrations of the cfa (french committee for antibiogram) and the mic of the main susceptible population of each species. the most frequent phenotypes were gtnt, tnta and gtnta. amikacin resistance including phenotypes were essentially found in klebsiella and serratia (35% and 53% of th ... | 1988 | 3043339 |
| [use of ceftazidime combined with netilmicin in the treatment of febrile episodes occurring after bone marrow transplantation in children]. | thirteen episodes of fever in bone marrow transplantation recipients (23 months to 11 years old children) were treated by ceftazidime (100-200 mg/kg/j) and netilmicin (7 mg/kg/j). vancomycin was added at the 24th hour in 10 cases of persistent fever. 6 presumed agents of infection were isolated before antibiotic treatment: blood cultures (streptococci 2, staphylococcus 1, proteus 1), fecal sample (e. coli 1), urine (e. coli 1). modifications of aerobic fecal flora were studied under this treatme ... | 1988 | 3043358 |
| proteus infection of the spine. | | 1988 | 3043721 |
| membrane oligosaccharides: structure and function during differentiation. | recent results gathered by normal light microscopy, immunocytochemistry, fluorescent-analog cytochemistry, and electron microscopy have allowed an improved interpretation of ameboid movement and related phenomena. 1. the contractile system responsible in amoeba proteus for the generation of motive force for protoplasmic streaming and a large variety of dynamic activities is represented mainly by a thin cortical filament layer at the cytoplasmic face of the cell membrane (fig. 18i). during normal ... | 1988 | 3045046 |
| lecithinase activity of proteus vulgaris. | lecithinase production is described as a new biochemical property of p. vulgaris strains grown in a selective agar medium containing brilliant green, crystal violet and lecithin (bcl agar), the authors' own modification of egg-yolk culture medium. by using this bcl agar as a medium inhibiting the swarming growth of p. vulgaris cultures the authors succeeded in identifying 12 lecithinase-positive strains among the p. vulgaris isolates obtained from patients with crohn's disease. of 50 p. mirabili ... | 1988 | 3045200 |
| [clinico-experimental study of a dried selective medium for isolating fungi in the genus candida]. | the trial of a newly developed dried selective medium for the isolation of fungi of the genus candida, involving the inoculation of 103 museum yeast strains and 542 specimens of pathological material, has been carried out. the data obtained in this trial indicate that the proposed medium has advantages over wort agar and sabouraud medium with antibiotics in the germination index, in the ability to ensure fungal growth after the inoculation of pathological material, and in selective properties. t ... | 1988 | 3046188 |
| neonatal meningitis due to proteus mirabilis: report of 3 cases. | | 1988 | 3046716 |
| a double-blind, multicenter, comparative study of the safety and efficacy of cefixime versus amoxicillin in the treatment of acute urinary tract infections in adult patients. | in this 31-site multicenter trial, 565 adult patients with urinary tract infections were randomly assigned to receive either a 10-day course of cefixime 400 mg once daily (n = 279) or amoxicillin 250 mg three times daily (n = 286). although all patients were included in the safety analysis, only 93 (33 percent) cefixime-treated and 99 (35 percent) amoxicillin-treated patients were fully evaluable for the efficacy analysis. one week after therapy, the evaluable patients treated with cefixime demo ... | 1988 | 3048090 |
| reduction of infection stones in rats by combined antibiotic and phosphocitrate therapy. | the potential of phosphocitrate to inhibit infection stones in rats when combined with an antibiotic was studied. a significant reduction occurred in both the number and weight of recovered stones from rats receiving combined treatment with amoxycillin (50 mg./kg. body wt./day and phosphocitrate (112 mumol./kg. body wt./day) for four weeks. the inhibitory responses were attributed to the intact phosphocitrate molecule as administration of citrate in equimolar concentrations did not mimic the obs ... | 1988 | 3050152 |
| effect of proteus mirabilis on liver and spleen weight and the hemagglutinin response to srbc in rats. | | 1988 | 3051246 |
| [characteristics of the clinical picture and differential diagnosis of intestinal proteus infection from sonne dysentery and gastrointestinal salmonellosis]. | | 1988 | 3051659 |
| corynebacterium group d2 and urolithiasis in a boy with megacalycosis. | this is report on a boy with megacalycosis in whom infectious urolithiasis after eradication of proteus mirabilis was maintained by corynebacterium group d2. | 1988 | 3053458 |
| comparison of the haemolysin secretion protein hlyb from proteus vulgaris and escherichia coli; site-directed mutagenesis causing impairment of export function. | the hlyb secretion genes of proteus vulgaris and escherichia coli showed 81% nucleotide homology and similar e. coli-atypical codon usage. the deduced protein sequences differed in 54 of 707 residues and shared a previously unreported sequence which corresponds to the atp-binding motif characteristic of protein kinases. the motif was also conserved in the hlyb of morganella morganii. of 4 oligonucleotide-directed substitutions introduced into the putative e. coli hlyb motif, 2 non-conservative c ... | 1988 | 3054490 |
| [detection of viral proteins during synthesis of defective phage of proteus mirabilis d52 using the immunocolloid gold method]. | we have applied the method of immunocolloidal gold in order to label the ribonuclear proteins of prokaryotic cells on isolated bacterial chromatosomes. in the process of protein synthesis it was possible to visualize a definite protein of defective phage d52 of proteus mirabilis. | 1988 | 3054506 |
| [in vitro antibacterial activity of apalcillin on aerobic bacteria and the regression curve]. | minimal inhibitory concentrations (mics) of apalcillin (apl) were evaluated by agar dilution on 1,201 bacterial strains isolated simultaneously in four university hospitals; agar diffusion tests (disks apl: 75 micrograms) were performed on these strains to establish relationship between mic and zone diameters. for enterobacteriaceae naturally non beta-lactamase-producing (e. coli and p. mirabilis), mode mic was 0.5 microgram/ml; some acquired penicillinase-producing strains were only inhibited b ... | 1988 | 3054743 |
| [clinical and bacteriological evaluation of cefmenoxime in the newborn infant]. | cefmenoxime (cmx) has been administered under parenteral injection to 39 neonates delivered at term, nearly always by monotherapy in an average dosage of 86.8 mg/kg/day. cmx has been used 31 times in first line therapy and 8 times after failure of association ampicillin-gentamicin. 25 strains have been identified: 16 e. coli (9 ampicillin resistant), 7 p. mirabilis (1 ampicillin resistant), 1 k. oxytoca and 1 streptococcus b. the neonates group with septicaemia (1 with arthritis) has been cured ... | 1988 | 3054762 |
| [a case using plasmapheresis in treating infectious toxicosis in young infants]. | | 1988 | 3054796 |
| hydrolytic rate at low drug concentration as a limiting factor in resistance to newer cephalosporins. | hydrolysis kinetics of two cephalosporinases from citrobacter freundii and proteus vulgaris having different affinities for cefotaxime and ceftazidime was assessed in studies with cefotaxime, ceftazidime, bmy-28142, and imipenem. the two cephalosporinase genes were cloned into strains of escherichia coli. the production of these cephalosporinases in strains of e. coli, as well as in the derepressed mutants of c. freundii and p. vulgaris, caused a decrease in susceptibility to the newer cephalosp ... | 1988 | 3055173 |
| inducible beta-lactamases: clinical and epidemiologic implications for use of newer cephalosporins. | the emergence of resistance to multiple beta-lactam antibiotics is a major problem in patients infected with organisms that characteristically produce inducible beta-lactamases--e.g., species of pseudomonas, enterobacter, serratia, citrobacter, indole-positive proteus, and providencia. resistance has emerged in 14%-56% of patients infected with these organisms and treated with one of the newer cephalosporins. the emergence of resistance has been associated with clinical failure or relapse in 25% ... | 1988 | 3055176 |
| paradoxical antibacterial activities of beta-lactams against proteus vulgaris: mechanism of the paradoxical effect. | fifteen beta-lactam antibiotics were divided into four classes based on their antibacterial actions and beta-lactamase-inducing activities in proteus vulgaris. one of these groups, which included cefmenoxime, ceftriaxone, cefuzonam, and cefotaxime, showed a clear paradoxical antibacterial activity against p. vulgaris. this group showed growth-inhibitory activity at relatively low concentrations, up to certain limits. these cephalosporins have, as a common moiety, an aminothiazolyl-oxyimino group ... | 1988 | 3056246 |
| [immunomodulating therapy of adjuvant disease in rats using cyclophosphamide in combination with prodigiozan--its effect on anti-infectious resistance]. | the effect of immunomodulating therapy of adjuvant disease in rats with cyclophosphamide, prodigiozan and their combinations on infection resistance, weight of the lymphoid organs and leukocyte counts in peripheral blood, as well as the effect of prodigiozan on acute toxicity of cyclophosphamide in intact mice and mice exposed to the freund's complete adjuvant (fca) was studied. prodigiozan did not increase acute toxicity of cyclophosphamide in the intact mice. it lowered the cyclophosphamide to ... | 1988 | 3056303 |
| brain abscesses in neonates. a study of 30 cases. | since the introduction of ultrasonography and computerized tomography (ct) scanning, brain abscesses are found more frequently in cases of neonatal meningitis and septicemia, particularly when the offending pathogen is proteus. thirty cases of brain abscess in neonates are reported, 27 of which were caused by proteus species infections. twenty infants had meningitis and 13 had septicemia. most of the abscesses were enormous, and multiple abscesses were observed in 17 cases. the frontal region wa ... | 1988 | 3057126 |
| variable in vitro activity of cefaclor, cephalothin and cefadroxil against escherichia coli, klebsiella pneumoniae and proteus mirabilis. | the in vitro activity of cefaclor, cephalothin and cefadroxil to 50 escherichia coli, 23 klebsiella pneumoniae and 20 proteus mirabilis strains was tested with the disc diffusion and microdilution plate techniques. cefaclor was more active than cefadroxil, while cephalothin was intermediate. the differences seriously affected the classification into the routine sir system. for susceptibility testing we question the use of cephalothin as a class representative of oral cephalosporins. | 1988 | 3057617 |
| chromosomal beta-lactamase expression and resistance to beta-lactam antibiotics in proteus vulgaris and morganella morganii. | indole-positive members of the proteeae usually have inducible expression of chromosomal beta-lactamases. mutants with stably derepressed beta-lactamase expression occur in inducible populations at frequencies in the range of 10(-6) to 10(-8). the contribution of these beta-lactamases to drug resistance was examined in morganella morganii and proteus vulgaris. the m. morganii enzyme was a high-molecular-weight (49,000) class i cephalosporinase with low vmax rates for ampicillin, carbenicillin, a ... | 1988 | 3058021 |
| [the frequency of isolating urinary infection germs at a community practice and their sensitivity to various antibiotics]. | nine hundred and thirty one urine's specimens of patients affected by urinary infection have been studied by pathology practising in different towns in france. the most frequently germs retrieved are: escherichia coli 76%, proteus mirabilis 12%, klebsiella 5%, staphylococcus epidermidis 2%. were studied gram negative rods sensibility to ampicillin (a), cephalosporin 1st generation (c), nalidixic acid (nal), gentamicin (g), norfloxacin (norf); gram positive cocci resistance to oxacillin (oxa), er ... | 1988 | 3059284 |
| case report 496: intraosseous gas in proteus mirabilis osteomyelitis complicating bone infarcts in sickle cell disease. | | 1988 | 3059500 |
| the in-vitro assessment of a collagen/vicryl (polyglactin) composite film together with candidate suture materials for potential use in urinary tract surgery. iii. adherence of bacteria to the material surface. | a collagen/vicryl (polyglactin) composite membrane (developed for use in urinary tract surgery) has been incubated in cultures of radioactively labelled urinary tract pathogens vis escherichia coli, staphylococcus epidermidis, and proteus mirabilis for up to 1 h. for comparison, collagen film, vicryl mesh, and a number of absorbable and non-absorbable sutures were similarly tested. following incubation, samples were also examined by scanning electron microscopy. under the experimental conditions ... | 1988 | 3059656 |
| [lysogeny in strains of proteus mirabilis and morganella morganii]. | | 1988 | 3060268 |
| identification of a novel plasmid-encoded dihydrofolate reductase mediating high-level resistance to trimethoprim. | a proteus mirabilis strain, isolated in south africa, carried a 79kb transferable plasmid (puk672) which encoded high-level resistance to trimethoprim (minimum inhibitory concentration greater than 2048 mg/l). the trimethoprim resistance was mediated by a dihydrofolate reductase which had completely different properties from any plasmid-encoded dihydrofolate reductase previously described. this enzyme has been designated type vi. the type vi enzyme is small (10,000 daltons) and is unstable in vi ... | 1988 | 3060457 |
| comparative in-vitro activity of a new oral carbacephem, ly163892. | the in-vitro activity of a new oral carbacephem, ly163892, was compared with cefaclor, cephalexin, cephradine, cefadroxil and selected penicillins against 529 bacterial isolates. ly163892 exhibited greater activity in vitro than all four cephalosporins against haemophilus influenzae, beta-lactamase producing branhamella catarrhalis, escherichia coli, klebsiella spp. and proteus mirabilis. ly163892 had equivalent potency to cefaclor against non-beta-lactamase producing b. catarrhalis, streptococc ... | 1988 | 3060458 |
| influence of antibiotic therapy on faecal carriage of p-fimbriated escherichia coli and other gram-negative bacteria in neonates. | the influence of previous antibiotic therapy on the aerobic faecal flora, including p-fimbriated escherichia coli, was studied in 953 neonates at discharge from 22 neonatal wards in sweden. antibiotics, mainly ampicillin (with or without gentamicin) or cefuroxime, had been received by 37% of the infants. treatment with ampicillin (with or without gentamicin) increased klebsiella/enterobacter and reduced esch. coli colonization. cephalosporin therapy (71% cefuroxime) reduced the frequency of colo ... | 1988 | 3060462 |
| [the safety of a vaccinal preparation made of bacteria of the genus proteus: the absence of a mutagenic effect]. | the protein-lipopolysaccharide vaccinal preparation, obtained by the disintegration of microorganisms of the genus proteus by means of hydrochloric hydroxylamine, has been tested for mutagenicity. two tests have been used: (1) the count of reversion to histamine-dependence in salmonella typhimurium cells, strains ta-98 and ta-100, intended for detecting mutations, such as read-through frame shift and, respectively, the change of nitrous bases in dna; and (2) the count of structural aberrations i ... | 1988 | 3061264 |
| further studies of swarmer cell differentiation of proteus mirabilis pm23: a requirement for iron and zinc. | proteus mirabilis pm23, unlike other motile strains of the species, differentiates in rich fluid media to form nonseptate filaments resembling the swarmer cells formed on solid media. the swarming activity of pm23 is greater than that of the other strains on solid media and it grows faster than another strain, im47, in differentiation-supporting broth. this faster growth is not exhibited in broth that does not support differentiation. the differentiation of pm23 in brain-heart infusion broth occ ... | 1988 | 3061618 |
| the microbial environment of the ear canal in health and disease. | otitis externa is an important disease of dogs and, to a lesser degree, cats. the yeast malassezia canis is the most common organism demonstrated in ear infections. coagulase-positive staphylococci are the most common bacteria isolated, often occurring with m. canis. pseudomonas aeruginosa and proteus are gram-negative bacteria that are frequently encountered. other bacteria, such as beta-hemolytic streptococci, enterococci, escherichia coli, and corynebacterium are also important. malassezia ca ... | 1988 | 3062878 |
| an examination of the anti-adherence activity of cranberry juice on urinary and nonurinary bacterial isolates. | in a previous investigation it was demonstrated that cranberry juice cocktail was able to inhibit adherence in 77 clinical isolates of escherichia coli obtained from patients with diagnosed urinary tract infections. this work has been extended to include clinical isolates of e. coli, proteus, klebsiella, enterobacter and pseudomonas isolated from urine, sputum, wound and stool. bacterial strains isolated from urine adhere in greater numbers to urinary tract epithelial cells than organisms isolat ... | 1988 | 3063927 |
| [in vitro studies of the effect of the components of stullmisan on the vitality of different gram-negative enterobacteria]. | | 1988 | 3064750 |
| clinical experience with ciprofloxacin in the treatment of urinary tract infections: a review. | during the clinical development of ciprofloxacin 1,519 treatments of uti were documented. the most frequent specific diagnoses were uncomplicated uti (46.6%), followed by non-specified uti (21.7%), complicated uti (19.4%), acute pyelonephritis (7.6%) and chronic pyelonephritis (4.1%). 70% of the causative organisms isolated were enterobacteriaceae (escherichia coli 38%, proteus spp. 10% and klebsiella pneumoniae 10%). pseudomonas aeruginosa occurred in approximately 20% of the cases and the rema ... | 1988 | 3065243 |
| identification of the promoters directing in vivo expression of hemolysin genes in proteus vulgaris and escherichia coli. | the hemolytic activity of escherichia coli and proteus vulgaris is determined by common contiguous genes encoding synthesis (hly c, hly a) and specific secretion (hly b, hly d) of active hemolysin. nevertheless, the hly c-proximal dna sequences directing production of the homologous hemolysins by the recombinant dnas p. vulgaris pvu763-709 and e. coli pann202-312 showed no extensive homology. primer extension and s1 nuclease protection were used to define in the two sequences the 5' termini of h ... | 1988 | 3065612 |
| [effect of copper, manganese and zinc on some causative agents of pyelonephritis in children]. | | 1988 | 3065714 |
| proticin sensitivity typing & proticin production by proteus. | | 1988 | 3068127 |
| [urinary levels of ammonium-magnesium phosphate and calcium phosphate in patients with infected urinary calculi]. | | 1988 | 3070500 |
| phagocytosis and killing of listeria by guinea pig macrophages and neutrophils. | the rate of the phagocytosis and intracellular killing of listeria monocytogenes by guinea pig macrophages and neutrophils in vitro was determined. the anti-bacterial activity of the phagocytes against virulent listeria monocytogenes was compared with their activity against avirulent strain of listeria and proteus mirabilis. it is suggested that the contribution of the macrophages and the neutrophils to anti-bacterial protection can depend on physiological state of bacteria. | 1988 | 3071297 |
| characterization of multiply resistant proteus mirabilis isolates in hungary. | according to a survey on tendencies of antibiotic resistance in hungary (1974-1983), the emergence of multiresistant (mr) proteus mirabilis isolates is a new phenomenon. a total of 60 strains resistant to 11-16 antibiotics were collected from various specimens and geographical locations. except for cefoperazone, third generation cephalosporins, amikacin, netilmicin and ofloxacin were effective in vitro against multiply resistant p. mirabilis isolates. out of them 62% belonged to o18, a serogroup ... | 1988 | 3072825 |
| [detection of hospital strains of proteus mirabilis by the methods of proticine and bacteriophage typing]. | | 1988 | 3073282 |
| typhus fever in south india--a case report. | | 1988 | 3074081 |
| routine burns bacteriology and proteus. | on standard agar media proteus spp. swarm rendering prompt detection of other organisms difficult. methods for preventing this have been investigated and a simple system devised suitable for inclusion in routine burns bacteriological investigations. its merits are discussed. | 1988 | 3074872 |
| experimental urolithiasis in the bladder of rats. | vesical foreign bodies readily lead to urolith formation but not in brattleboro rats with diabetes insipidus. this experiment was designed to examine the roles of vesical foreign bodies, urine osmolality, and vesical infection in the formation of uroliths and associated reactive urothelial hyperplasia. adh-deficient brattleboro rats received surgical insertion of a silk suture in the bladder. their bladders were infected with intravesical injection of urease positive p. vulgaris, while controls ... | 1988 | 3075986 |
| mathematical modelling of intercellular regulation causing the formation of spatial structures in bacterial colonies. | bacterial colonies may grow forming stable spatial, particularly circular, structures. for instance, motile bacteria proteus vulgaris or escherichia coli grown on agar under certain conditions may form concentric rings with the centre in the inoculation point (rüss-münzer, 1935, bact. parasit kde (abt 1) 7, 214; budriené, 1985, dokl. acad. nauk ssr, 283, 470). a similar picture can be observed in a different situation, i.e. when a lawn of non-motile salmonella typhimurium bacteria is cultivated ... | 1988 | 3076912 |
| [urinary tract infections: bacteriology, treatment, and antibody- coated bacteria]. | | 1988 | 3077241 |
| [sensitivity of proteus mirabilis strains to the bactericidal action of human serum]. | | 1986 | 3077431 |
| [characteristics of proteus mirabilis hemolysins]. | | 1986 | 3078173 |
| [clinical experience with imipenem/cilastatin in the treatment of severe infections in general surgery]. | in a prospective study 43 patients (19 men, 24 women) suffering from severe bacterial infections such as peritonitis (n = 16), soft tissue infection (n = 12), pneumonia (n = 7), septicemia (n = 6), catheter sepsis (n = 2), cholangitis (n = 4), osteomyelitis (n = 3), complicated urinary tract infection (n = 2) or endocarditis (n = 1) were treated t. i. d. with short-time i. v. infusions of 0.5 g imipenem/cilastatin for five to 37 days (means = 9). all the patients were cured or significantly impr ... | 1986 | 3079549 |
| in vitro studies of the synergism of piperacillin and netilmicin against blood culture isolates. | the purpose of this study was to evaluate the in vitro synergism between piperacillin and netilmicin against microorganisms isolated from danish patients with septicemia and to examine the influence of inactivation of piperacillin among these bacteria on the synergy results. a total of 132 stains was examined: escherichia coli 20, indole-positive proteus 17, klebsiella pneumoniae 18, enterobacter cloacae 20, pseudomonas aeruginosa 20, staphylococcus aureus 20, and coagulase-negative staphylococc ... | 1986 | 3081304 |
| providencia stuartii: a common cause of antibiotic-resistant bacteriuria in patients with long-term indwelling catheters. | the long-term-catheterized urinary tract may offer a particular niche to providencia stuartii, which is otherwise an uncommon clinical isolate. published accounts of bacteriuria in patients catheterized for long periods indicate that p. stuartii has often been found as frequently as familiar uropathogens such as escherichia coli, proteus mirabilis, enterococcus, and pseudomonas aeruginosa. as in most nosocomial infections, the frequency of isolation of a given species has commonly differed among ... | 1986 | 3081988 |
| bacterial colonization of hemasite access devices. | vascular access ports (hemasites) were recovered from patients in whom they had become foci of infection and were examined according to microbiologic and morphologic techniques. all were covered on their extraluminal surfaces by well-developed biofilms consisting of host material and bacteria and their extracellular products. one hemasite from which staphylococcus aureus and streptococcus faecalis were cultured was covered by a biofilm that consisted of coccoid bacterial cells and occasional fun ... | 1986 | 3082026 |
| [indoor environmental contamination of a trauma hospital by opportunistic gram-negative microorganisms and an evaluation of methods for their detection]. | | 1986 | 3082722 |