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the inhibition of the spreading growth of proteus and other bacteria to permit the isolation of associated streptococci. 194116560477
brucella, pasteurella tularensis, and proteus agglutinins in chronic brucellosis. 194116560426
temperature activation of the urease-urea system using urease of proteus vulgaris. 194116560420
utilization of nicotinic acid and related pyridine compounds by the proteus group of organisms. 194016560303
the pleo-antigenicity of a variant of proteus x19. 193416559764
some conditions affecting the production of gelatinase by proteus bacteria. 192816559306
alterations of the l-forms of a sporebearing bacillus.a large pleomorphic gram-negative bacillus developed as a contaminant on blood-agar. spores were formed in one culture. l-forms were produced with penicillin on blood-agar with 2.5% nacl; they grew well when transplanted to agar with 0.5% nacl. after several transplants and long incubation of the l-forms without penicillin, in three transplants small gram-negative pleomorphic bacilli grew, but no l-forms. this occurred once on blood-agar and twice on 30% gelatin. the growth obtained from these s ...197016559116
permanent alterations of the l-forms of proteus and salmonella under various conditions.l-forms obtained from three strains of proteus and from one strain of salmonella have been kept for 15 to 20 years by weekly or monthly transfers on agar plates containing penicillin. the morphology and growth requirements of these strains have changed. they now grow abundantly on the surface of agar and in broth. the cultures consist of large bodies, small granules, and transitional forms. these organisms are more resistant to distortion and stain more deeply than organisms of the usual l-forms ...197016559115
a systematic study of the proteus group of bacteria. 191916558845
cephalexin: absorption and excretion as related to renal function and hemodialysis.cephalexin is a new semisynthetic cephalosporin c derivative, which is well absorbed from the gastrointestinal tract and excreted in high concentration in the urine. even in patients with impaired renal function, the concentrations of cephalexin present in the urine are adequate for the treatment of most urinary tract infections produced by escherichia coli, klebsiella, and proteus mirabilis. in anephric patients, single doses of 250 or 500 mg of cephalexin resulted in high, prolonged serum conc ...197116558013
factors affecting the transmission of salmonella by flies: natural resistance to colonization and bacterial interference.groups of immobilized gnotobiotic houseflies, musca domestica l., were mono-contaminated with graded doses of salmonella typhimurium, bicontaminated with s. typhimurium and proteus mirabilis, and multicontaminated with s. typhimurium and a mouse fecal flora. of 45 flies fed an average of 22 salmonella cells alone, 26 showed bacterial multiplication, up to 1.4 x 10(7) in one case. there was a fairly consistent increase in percentage of salmonella excreters with increasing input, so that all flies ...197016557919
agar-gel precipitating antibody in pseudomonas aeruginosa infections.the human immune response to pseudomonas aeruginosa infection was studied by using the double diffusion in agar-gel technique. antigens from fisher-devlin-gnabasik immunotypes were prepared by both trichloroacetic acid extraction and ultrasonic disruption. serum from 72 of 168 patients (43%) from whom p. aeruginosa was isolated formed from one to eight precipitin bands. precipitins were demonstrated in the sera of 60 of 66 (91%) patients recovering from bacteremia and deep infections; however, t ...197016557868
effect of mg and ethylenediaminetetraacetate on the in vitro activity of coumermycin a(1) and novobiocin against gram-negative bacteria.the effect of mg(2+) and ethylenediaminetetraacetic acid (edta) on the in vitro activity of coumermycin a(1) and novobiocin was determined against pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, and proteus mirabilis grown in trypticase soy broth or defined synthetic medium. in the presence of edta, the activity of coumermycin a(1) was significantly enhanced (8- to 40-fold) against all four organisms in the synthetic medium lacking mg(2+); novobiocin activity was increased to a ...197016557848
prevention of experimental hematogenous and retrograde pyelonephritis by antibodies against enterobacterial common antigen.rabbits were immunized with common antigen (ca) derived from salmonella typhimurium. animals with ca hemagglutinin titers of 1-320 to 1-81,920 were injected with 10(7) viable proteus mirabilis via the retrograde and hematogenous routes. nonimmune control groups were challenged similarly. from the retrogradely challenged groups sacrificed at 4 weeks, pyelonephritis was found in 89% of the control animals but not in those immunized. bacteriuria was present in 89% of the controls but in only 61% of ...197016557817
conditions affecting the viability of spheroplasts in urine.penicillin-induced spheroplasts from escherichia coli and proteus vulgaris, which at neutral ph had little resistance to osmotic lysis, retained viability in media of low osmolality at ph 5.0 to 5.5. in the presence of 1,000 iu of penicillin per ml (ph 5.0) in liquid media, e. coli remained in rod form without conspicuous growth. on hypotonic agar media at ph 5.2 with 1,000 iu of penicillin per ml, the bacteria grew out as l-type colonies. these findings are discussed in relation to penicillin t ...197016557732
proteus syndrome with huge tonsillar mass causing dysphagia: a rare case.proteus syndrome is a rare and highly variable hamartomatous syndrome that can affect multiple organ systems. it is characterized by hyperplasia of connective tissue, vascular malformations, epidermal naevi and hyperostosis. most of the cases present to plastic and orthopaedic surgeons. otolaryngologic presentations are minimal. we report such a rare case, which presented with a huge unilateral tonsillar mass causing dysphagia.200616556346
urinary tract infections in general practice patients: diagnostic tests versus bacteriological culture.urinary tract infections (utis) are common bacterial infections encountered in general practice. for the optimal treatment the general practitioner (gp) should rely on the results of diagnostic tests and recent antimicrobial susceptibility of uropathogens.200616554337
the presence of a conjugative gram-positive tn2009 in gram-negative commensal bacteria.to determine whether mef(a)-msr(d) and tet(m) genes are linked in representative gram-negative isolates and/or transferred together during conjugation. to molecularly characterize the acinetobacter junii element and compare the structure and sequence with the non-conjugative streptococcus pneumoniae tn2009 element.200616551693
bioactive components of the traditionally used mushroom podaxis pistillaris.in the course of an ethnobotanical study on fungi used in yemeni ethnomedicine the fungus podaxis pistillaris (podaxales, podaxaceae, basidiomycetes) was found to exhibit antibacterial activity against staphylococcus aureus, micrococcus flavus, bacillus subtilis, proteus mirabilis, serratia marcescens and escherichia coli. in the culture medium of p. pistillaris three epidithiodiketopiperazines were identified by activity-guided isolation. based on spectral data (nmr, esi-ms and dci-ms) their id ...200616550228
effect of steroid on mitochondrial oxidative stress enzymes, intestinal microflora, and bacterial translocation in rats subjected to temporary liver inflow occlusion.protective effects of steroids against ischemia-reperfusion (i/r) injury are well known, but there is little information about the influence of temporary inflow occlusion on intestinal barrier function or bacterial translocation. the aim of this experimental study was to investigate the effects on liver, kidney, spleen, ileal mitochondrial stress enzymes, and bacterial translocation of methylprednisolone (mp) in rats undergoing temporary liver inflow occlusion. twenty-seven pathogen-free wistar ...200616549125
increasing trend in the prevalence of plasmid-mediated ampc beta-lactamases in enterobacteriaceae lacking chromosomal ampc gene at a korean university hospital from 2002 to 2004.the aim of the study is to investigate the prevalence of plasmid-mediated ampc beta-lactamases in enterobacteriaceae naturally lacking chromosomal ampc beta-lactamases. a total of 1860 clinical isolates of klebsiella spp., salmonella spp., and proteus mirabilis were collected from a korean hospital between january 2002 and december 2004. for the isolates that are nonsusceptible to cefoxitin, polymerase chain reaction amplification of the bla(shv), bla(tem), and bla(ampc) genes and sequencing wer ...200616545935
isolation of enterobacter sakazakii from stable flies, stomoxys calcitrans l. (diptera: muscidae).enterobacter sakazakii is an opportunistic foodborne pathogen that causes meningitis, enterocolitis, and sepsis, primarily in immunocompromised infants. previously, it was suggested that stable flies, stomoxys calcitrans, were a vector or reservoir of this pathogen. in our study, by means of a culturing approach combined with 16s rdna pcr-restriction fragment length polymorphism genotyping and sequencing, we screened 928 individual stable flies collected in kansas and florida. two stable flies ( ...200616541702
optimization of ferrioxamine e concentration as effective supplementation for selective isolation of salmonella enteritidis in egg white.utilization of ferrioxamine e (fe) as a sole source of iron distinguishes salmonella from a number of related species, including escherichia coli. fe is not able to serve as a source of iron for e. coli or the proteus-providencia-morganella group. this confers a selective advantage on salmonella enteritidis in egg white supplemented with fe. the optimum concentration of fe that promoted a selective advantage for salmonella in egg white was determined. four supplementation concentrations were eva ...200616541696
inhibitory effects of secondary metabolites from the red alga delisea pulchra on swarming motility of proteus mirabilis.abnormal, uncoordinated swarming motility of the opportunistic human pathogen proteus mirabilis was seen when a crude extract of the australian red alga delisea pulchra was added to the medium. this occurred at concentrations at which growth rate, swimming motility, cell elongation, polynucleation, and hyperflagellation were not affected. one halogenated furanone from d. pulchra inhibited swarming motility at concentrations that did not affect growth rate and swimming motility. other structurall ...199616535454
[protective effect of the aerosol immunization of mice with the polycomponent vaccine immunovac vp-4 after the challenge of the animals with klebsiella pneumoniae virulent strain].used four schemes of the administration of the preparation with different time of the exposition of the animals in an aerosol chamber were tested with their subsequent intraperitoneal challenge with k. pneumoniae virulent strain k16. irrespective of the number of immunization courses, the administration of the preparation made at intervals of 1 day, or daily, did not ensure any protective effect, but only led to an insignificant increase in their survival time in comparison with nonimmunized ani ...200616532635
[proteus syndrome with cerebral vascular malformations].proteus syndrome is a congenital hamartomatous dysplasia. this sporadic disorder involves the skeletal system, soft tissues, skin and vascular system. the most likely pathogenesis involves somatic mosaicism. main manifestations included soft-tissue and epidermal nevi, partial gigantism, hemihypertrophy, exostoses, lipomas and vascular anomalies. the most common brain abnormalities are hemimegencephaly and migrational disorders. we present a case of proteus syndrome with cerebral vascular anomali ...200616525914
global assessment of the antimicrobial activity of polymyxin b against 54 731 clinical isolates of gram-negative bacilli: report from the sentry antimicrobial surveillance programme (2001-2004).in total, 54 731 gram-negative bacilli isolated worldwide between 2001 and 2004 from diverse sites of infection were tested for susceptibility to polymyxin b by the broth reference microdilution method, with interpretation of results according to clsi (formerly nccls) guidelines. polymyxin b showed excellent potency and spectrum against 8705 pseudomonas aeruginosa and 2621 acinetobacter spp. isolates (mic50, < or = 1 mg/l and mic90, 2 mg/l for both pathogens). polymyxin b resistance rates were s ...200616524407
[necrotizing fasciitis of the head and neck].necrotizing fasciitis (nf) is a potentially lethal soft tissue infection characterized by cutaneous necrosis, suppurative fasciitis, vascular thrombosis and extreme systemic toxicity. it is a rare entity in the head and neck region, but occur most frequently in patients with diabetes and chronic alcoholism. mostly involved are immunodeficient patients with banal infections of the upper aerodigestive tract, small traumas, but also after surgical procedures. necrotizing fasciitis is an infection c ...200516521446
[nationwide surveillance of parenteral antibiotics containing meropenem activities against clinically isolated strains in 2004].the antibacterial activity of meropenem (mepm) and other parenteral antibiotics against clinical isolates of 907 strains of gram-positive bacteria, 1790 strains of gram-negative bacteria, and 192 strains of anaerobic bacteria obtained from 30 medical institutions during 2004 was measured. the results were as follows; 1. mic90 of mepm for almost all of enterobacteriaceae and haemophilus influenzae were 4-fold to 32-fold lower than those of other carbapenems. mepm was more active than other carbap ...200516521347
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (2003). i. susceptibility distribution].the bacterial strains isolated from 565 patients diagnosed as having urinary tract infections (utis) in 14 institutions in japan were collected between august 2003 and july 2004. the susceptibilities of them to many kinds of antimicrobial agents were investigated. of them, 701 strains were estimated as prophlogistic bacteria and used for the investigation. the strains consisted of 258 gram-positive bacterial strains (36.8%) and 443 gram-negative bacterial strains (63.2%). against staphylococcus ...200516521344
evaluation of the phoenix 100 id/ast system and nid panel for identification of enterobacteriaceae, vibrionaceae, and commonly isolated nonenteric gram-negative bacilli.the phoenix 100 id/ast system (becton dickinson co., sparks, md.) is an automated system for the identification and antimicrobial susceptibility testing of bacterial isolates. this system with its negative identification (nid) panel was evaluated for its accuracy in the identification of 507 isolates of the family enterobacteriaceae, 57 other nonenteric gram-negative isolates that are commonly isolated in clinical microbiology laboratories, and 138 isolates of the family vibrionaceae. all of the ...200616517878
gastric microbiota in elderly patients fed via nasogastric tubes for prolonged periods.aspiration of oropharyngeal contents is a constant threat for elderly patients fed via a nasogastric tube (ngt). colonization of the oropharynx of these patients by pathological flora and the development of biofilms on the feeding tube has been documented recently. in addition, the presence of the ngt may interfere with the gastro-oesophageal sphincter, leading to gastro-oesophageal reflux. thus, the passage of pathogenic bacteria in both directions is facilitated. the purpose of this study was ...200616516342
crystallization and preliminary x-ray diffraction analysis of a cold-adapted catalase from vibrio salmonicida.catalase (ec 1.11.1.6) catalyses the breakdown of hydrogen peroxide to water and molecular oxygen. recombinant vibrio salmonicida catalase (vsc) possesses typical cold-adapted features, with higher catalytic efficiency, lower thermal stability and a lower temperature optimum than its mesophilic counterpart from proteus mirabilis. crystals of vsc were produced by the hanging-drop vapour-diffusion method using ammonium sulfate as precipitant. the crystals belong to the monoclinic space group p2(1) ...200616511268
crystallization of truncated hemolysin a from proteus mirabilis.proteus species are second only to escherichia coli as the most common causative agent of gram-negative bacteria-based urinary-tract infections and many harbor several virulence factors that provide inherent uropathogenicity. one virulence factor stems from a two-partner secretion pathway comprised of hemolysin a and hemolysin b; upon hemolysin b-dependent secretion, hemolysin a becomes activated. this system is distinct from the classic type i secretion pathway exemplified by the hemolysin syst ...200516511066
choice of antibiotic for empirical therapy of acute cystitis in a setting of high antimicrobial resistance.a high prevalence of antimicrobial resistance among urinary isolates in the garhwal region of uttaranchal.200616505574
[antimicrobial susceptibility of aerobic bacteria isolated from leishmaniotic ulcers in corte de pedra, ba].a prospective study regarding aspects of antimicrobial susceptibility aspects was realized among patients with tegumentary leishmaniasis in corte de pedra, bahia. cases were composed mainly of adolescent and adult farmer patients with single lesions. staphylococcus aureus predominated (83%) in the cultures with susceptibility to the majority of antibiotics. a mixed bacterial flora in ulcers was encountered in 37 (44.1%) patients. among the gram-negative bacteria isolated, enterobacter sp (13.1%) ...200616501766
bacteria and the gastrointestinal tract: beneficial and harmful effects.bacteria are unique microorganisms that have a variety of physiological functions which are beneficial to human beings. however, bacteria can also be harmful and cause infections if translocated from the gastrointestinal tract to the epithelial tissue following surgery. the process of translocation can lead to surgical wounds becoming contaminated with gastrointestinal microbes such as escherichia coli, proteus or klebsiella species. bacteria can also cause severe gastrointestinal infections as ...200616493321
detection of diverse bacterial signatures in atherosclerotic lesions of patients with coronary heart disease.bacterial infection has been discussed as a potential etiologic factor in the pathophysiology of coronary heart disease (chd). this study analyzes molecular phylogenies to systematically explore the presence, frequency, and diversity of bacteria in atherosclerotic lesions in patients with chd.200616490835
the potential use of antibacterial peptide antibody indices in the diagnosis of rheumatoid arthritis and ankylosing spondylitis.both rheumatoid arthritis (ra) and ankylosing spondylitis (as) are potentially disabling arthritic disorders for which as yet no highly sensitive and reliable diagnostic laboratory markers are available.200616484874
increasing incidence of bacterial, resistance to antibiotics by isolates from the urinary tract.this is a prospective study to determine the prevalence of antibiotic resistance among organisms causing urinary tract infections in a teaching hospital between august 2003 and july 2004 and to compare them with an earlier study in 1993. a total of 1,104 urine samples were collected in sterile universal containers from patients attending university of nigeria teaching hospital and studied. all samples showing significant bacteriuna were studied and isolates identified using standard bacteriogica ...200516477864
functional grouping based on signatures in protein termini.the two ends of each protein are known as the amino (n-) and carboxyl (c-) termini. short signatures in a protein's termini often carry vital cellular function. no systematic research has been conducted to address the importance of short signatures (3 to 10 amino acids) in protein termini at the proteomic level. specifically, it is unknown whether such signatures are evolutionarily conserved, and if so, whether this conservation confers shared biological functions. current signature detection me ...200616475191
antibiotic treatment and resistance of unselected uropathogens in the elderly.a population-based study was conducted among women over the age of 70 years with complaints of uncomplicated urinary tract infections (utis). the positive predictive value of the nitrite test alone or in combination with the leukocyte esterase test ranged between 83% and 99%. the nitrofurantoin prescription rate decreased whereas fluoroquinolone and amoxicillin/clavulanic acid prescriptions increased with increasing age. the aetiology of infection was age-dependent. escherichia coli was the most ...200616473503
biodegradation and decolourization of anaerobically treated distillery spent wash by a novel bacterial consortium.the aim of this study was to isolate microorganisms capable of decolourizing and degrading anaerobically treated distillery spent wash. a bacterial consortium dmc comprising of three bacterial cultures was selected on the basis of rapid effluent decolourization and degradation, which exhibited 67 +/- 2% decolourization within 24 h and 51 +/- 2% chemical oxygen demand reduction within 72 h when incubated at 37 degrees c under static condition in effluent supplemented with 0.5% glucose, 0.1% kh(2) ...200716473005
clonal occurrences of multidrug-resistant gram-negative bacilli: report from the meropenem yearly susceptibility test information collection surveillance program in the united states (2004).the meropenem yearly susceptibility test information collection program is a global, longitudinal resistance surveillance network of more than 100 medical centers worldwide monitoring the susceptibility of bacterial pathogens to carbapenems and other broad-spectrum agents. between 1999 and 2004, a total of 10-16 us medical centers referred up to 200 nonduplicate isolates from clinical infections to a central processing laboratory. over this 6-year period, the antimicrobial activity of 12 broad-s ...200616466890
misdiagnosis of neurofibromatosis type 1 as proteus syndrome. 200516462730
cowden disease and lhermitte-duclos disease: an update. case report and review of the literature.cowden disease is a rare autosomal-dominant phacomatosis and cancer syndrome that is associated with lhermitte-duclos disease (ldd), also called dysplastic cerebellar gangliocytoma.200616459996
aerobic heterotrophic bacterial diversity in sediments of rajakkamangalam estuary, south west coast of india.the abundance of aerobic heterotrophic bacterial population in the sediment sample of six experimental stations (s1 to s6) at rajakkamangalam estuary was studied for a period of one year from march, 1998 to february, 1999. the important bacterial genera encountered were pseudomonas, bacillus, vibrio, escherichia, micrococcus, enterobacter, salmonella, aeromonas, shigella, klebsiella, proteus, alkaligenes, staphylococcus, citrobacter and flavobacterium. the bacterial population based on numerical ...200516459565
mechanical transmission of pathogenic organisms: the role of cockroaches.cockroaches (diploptera punctata) are basically tropical insects and will do their best to find a home that is both warm and moist. their involvement in the transmission of tropical diseases is poorly investigated in africa.200516457381
use of electrochemical dna biosensors for rapid molecular identification of uropathogens in clinical urine specimens.we describe the first species-specific detection of bacterial pathogens in human clinical fluid samples using a microfabricated electrochemical sensor array. each of the 16 sensors in the array consisted of three single-layer gold electrodes-working, reference, and auxiliary. each of the working electrodes contained one representative from a library of capture probes, each specific for a clinically relevant bacterial urinary pathogen. the library included probes for escherichia coli, proteus mir ...200616455913
isolation of salmonella bacteriophages from swine effluent lagoons.bacteriophages (phages) associated with salmonella were collected from nine swine manure lagoons in mississippi. phages were isolated by an enrichment protocol or directly from effluent. for enrichment, chloroform-treated samples were filtered (0.22 mum) and selectively enriched by adding a cocktail of salmonella strains in trypticase soy broth. after overnight incubation at 35 degrees c, chloroform was added and samples stored at 5 degrees c. enriched samples were tested by double agar layer (d ...200616455853
tryptophanase from proteus vulgaris: the conformational rearrangement in the active site, induced by the mutation of tyrosine 72 to phenylalanine, and its mechanistic consequences.tyr72 is located at the active site of tryptophanase (trpase) from proteus vulgaris. for the wild-type trpase tyr72 might be considered as the general acid catalyst at the stage of elimination of the leaving groups. the replacement of tyr72 by phe leads to a decrease in activity for l-tryptophan by 50,000-fold and to a considerable rearrangement of the active site of trpase. this rearrangement leads to an increase of room around the alpha-c atom of any bound amino acid, such that covalent bindin ...200616455316
factors affecting crystal precipitation from urine in individuals with long-term urinary catheters colonized with urease-positive bacterial species.weekly urinalysis was conducted for 12 weeks on a group of 21 long-term catheter users with confirmed catheter encrustation and urinary tract colonization with urease-positive bacteria, in order to explore the cause of considerable variation in the severity of encrustation between sufferers. the rapidity of catheter blockage correlated significantly with the ph above which crystals precipitated from urine (the nucleation ph) but not the ph of the voided urine itself. linear regression showed the ...200616453146
a new gastrointestinal finding in proteus syndrome: report of a case of multiple colonic hemangiomas.a patient with proteus syndrome presented with lower gastrointestinal bleeding due to multiple colonic hemangiomas, a finding which has not been described previously in this syndrome. the patient was a 20-year-old man with features fulfilling the diagnostic criteria for proteus syndrome. he fulfilled both general criteria (mosaic distribution of the lesions, progressive course and sporadic occurrence) and specific criteria (including epidermal nevus, disproportionate overgrowth of limbs and vasc ...200616445505
mössbauer identification of a protonated ferryl species in catalase from proteus mirabilis: density functional calculations on related models.the proteus mirabilis catalase is one of the most efficient heme-containing catalase and forms a relatively stable compound ii. samples of compound ii were prepared from pmc enriched in (57)fe. for the first time, two different forms of compound ii, namely low ph compound ii (lph ii) (43%) and high ph compound ii (hph ii) (25%), have been characterized by mössbauer spectroscopy at ph 8.3. the ratio lph ii/hph ii increases irreversibly with decreasing ph. the large quadrupole splitting value of l ...200616442627
windblown foot: a particular presentation of proteus syndrome? 200516440890
[influence of dendrite cells, generated with the use of immunomodulators of microbial origin, on the proliferative and cytotoxic activity of lymphocytes].the influence of ripe dendrite cells (dc) on the proliferative activity of mononuclear leukocytes and the cytotoxic activity of lymphocytes of syngenic mice was studied. as inducers of dc ripening, the combination of antigenic components incorporated into the vaccine "lmmunovac bh-4", (klebsiella pneumoniae, proteus vulgaris, escherichia coli, staphylococcus aureus), as well as k. pneumoniae lps and tnf-alpha, were used. this study demonstrated that dc activated with these preparations enhanced ...200516438373
[evaluation of the antimicrobial potential of immune sera].the inhibiting activity of blood sera obtained from 20 volunteers immunized with the multi-component vaccine "pyopol", 25 nonimmune donors and 7 lots of human antistaphylococcal immunoglobulin with respect to staphylococcus aureus, escherichia coli, proteus vulgaris and pseudomonas aeruginosa has been studied in the liquid nutrient medium with the use an automated microbiological analyzer. the sera of donors immunized with the vaccine "pyopol" have been found to possess high antimicrobial activi ...200516438372
detection and identification of intestinal pathogenic bacteria by hybridization to oligonucleotide microarrays.to detect the common intestinal pathogenic bacteria quickly and accurately.200516437687
lymphocytic colitis: a clue to bacterial etiology.to find out the role of bacteria as a possible etiological factor in lymphocytic colitis.200516437626
cmy-16, a novel acquired ampc-type beta-lactamase of the cmy/lat lineage in multifocal monophyletic isolates of proteus mirabilis from northern italy.we report multifocal detection (four different cities in northern italy) of proteus mirabilis isolates resistant to both oxyimino- and 7-alpha-methoxy-cephalosporins and producing a novel acquired ampc-like beta-lactamase. the enzyme, named cmy-16, is a variant of the cmy/lat lineage, which differs from the closest homologues, cmy-4 and cmy-12, by a single amino acid substitution (a171s or n363s, respectively) and from cmy-2 by two substitutions (a171s and w221r). expression of the cloned bla(cm ...200616436718
pharmacodynamic modeling of imipenem-cilastatin, meropenem, and piperacillin-tazobactam for empiric therapy of skin and soft tissue infections: a report from the optama program.the bactericidal exposures necessary for positive clinical outcomes among skin and soft tissue infections are largely dependent on interpatient pharmacokinetic variability and pathogen drug susceptibility. by simulating the probability of achieving target bactericidal exposures, the pharmacodynamics of three beta-lactam agents were compared against a range of pathogens implicated commonly in complicated skin and soft tissue infections.200516433606
capillary activity of surgical sutures and suture-dependent bacterial transport: a qualitative study.to evaluate the multitude of new synthetic absorbable sutures (both monofilament and multifilament) in comparison with older materials with regard to capillarity and bacterial transport.200516433602
surgical treatment of an unusual patient with proteus syndrome. 200616432429
erythromycin synergism with essential and trace elements.in order to establish the role of various essential and trace element complexation on the antibacterial activity of various macrolide antibiotics, the synergistic or antagonistic behavior of erythromycin metal complexes have been studied and compared with the parent drug. metal complexes of erythromycin with magnesium, calcium, chromium, manganese, iron, cobalt, nickel, copper, zinc and cadmium have been investigated for their antibacterial activity and compared with erythromycin by observing th ...200516431396
characterization of the brewery spoilage bacterium obesumbacterium proteus by automated ribotyping and development of pcr methods for its biotype 1.to study the ability of automated ribotyping to characterize obesumbacterium proteus and hafnia alvei, to design primers and to evaluate standard end-point and real-time pcr for the detection of o. proteus biotype 1 in beer and in brewers's yeast-containing samples.200616430517
radiographic and ultrasonographic findings of emphysematous cystitis in four nondiabetic female dogs.the urinary bladder of four dogs with emphysematous cystitis was assessed radiographically. ultrasonography was also performed using a 7.5-mhz microconvex probe in dorsal recumbency and in a standing position. ultrasonographically there were bright echoes and reverberations typical of gas in all dogs. this was entrapped in the bladder wall as it appeared in the same location in recumbent and standing positions. bladder size was reduced and bladder content was echogenic in all dogs. in only one o ...200616429992
the first detection of ctx-m-14 extended-spectrum beta-lactamase among diverse beta-lactamase-producing proteus mirabilis clinical isolates.ctx-m-14- and -2-like extended-spectrum beta-lactamases were detected in 5 and 1 proteus mirabilis isolates, respectively, among 92 non-duplicate strains tested from december 2002 to september 2003. this is the first report of the ctx-m-14 enzyme in p. mirabilis to the best of our knowledge and suggests that the ctx-m-type enzyme is gradually spreading to this species. bla(tem), bla(shv-1), and/or bla(oxa-10) alleles were also detected in 35 ampicillin-resistant isolates tested.200616427241
perioperative respiratory complications caused by cystic lung malformation in proteus syndrome.a 7-year-old girl with proteus syndrome, complicated with severe kyphoscoliosis and cystic lung malformation, developed respiratory complications during and after posterior spinal fusion under general anesthesia. we speculated that low oxygenation while in the prone position contributed to ventilation perfusion mismatch owing to the cystic lung malformation that existed predominantly in the right lung, and that postoperative respiratory failure was caused by initially increased bronchial mucous ...200616421672
rigid sigmoidoscopy: a potential hazard for cross-contamination.rigid sigmoidoscopy using a disposable or nondisposable sigmoidoscope is a common outpatient procedure. it has been assumed that the nondisposable bellows and light head of the sigmoidoscope remain free from enteric organisms so that the procedure is sterile if a disposable or nondisposable (metal) sigmoidoscope shaft is used. the aim of this study was to identify the presence of organisms within the bellows or light head of the sigmoidoscope.200616418794
[rare bacteria species found in wounds of tsunami patients. predominance of gram-negative rods, increased antibiotic resistance].microbiological cultures from 229 patients seeking medical advise in stockholm after the tsunami catastrophe december 2004 were analysed at the clinical microbiology laboratory, karolinska university hospital, stockholm, sweden. gram-negative rods were the most common findings from wound cultures. common human pathogens as escherichia coli, proteus species, klebsiella spp, and pseudomonas aeruginosa were isolated. however, more rare species of gram-negative rods were also isolated, e.g. myroides ...200516416946
comparative analysis of a modified ecolite method, the colicomplete method, and a most-probable-number method for detecting escherichia coli in orange juice.the ecolite high volume juice (hvj) presence-absence method for a 10-ml juice sample was compared with the u.s. food and drug administration bacteriological analytical manual most-probable-number (mpn) method for analysis of artificially contaminated orange juices. samples were added to ecolite-hvj medium and incubated at 35 degrees c for 24 to 48 h. fluorescent blue results were positive for glucuronidase- and galactosidase-producing microorganisms, specifically indicative of about 94% of esche ...200616416921
evaluation of a novel kit for the rapid detection of extended-spectrum beta-lactamases.since a method of rapidly detecting extended-spectrum beta-lactamase (esbl) production in gram-negative isolates from patients with severe infection is urgently required, the present study of a novel commercial kit was conducted. the cica-beta test i (kanto chemical, tokyo, japan) is designed for the rapid detection of esbl in gram-negative bacteria directly from isolated colonies in a 15-min protocol. in this study, a total of 304 strains of klebsiella spp., escherichia coli and proteus mirabil ...200616416268
a study of sensitivity and resistance of pathogenic micro organisms causing uti in kathmandu valley.a retrospective study was conducted in five hospitals to observe the prevalence of organisms causing uti and their sensitivity to antibiotics. methodology: altogether, data from five hundred samples of urine from five hospitals in kathmandu was collected for this study from january 2005 to april 2005. result: a total of 244 samples were found to be positive. altogether six types of organisms were isolated as the causative factors. e. coli (49%), s. aureus, (coagulase positive) (23%), proteus spe ...200516415607
clarithromycin synergism with essential and trace elements.in order to ascertain the role of various essential and trace element complexation on the antibacterial activity of various macrolide antibiotics, the synergistic or antagonistic behavior of clarithromycin metal complexes has been studied and compared with the parent drug. metal complexes of clarithromycin with magnesium, calcium, chromium, manganese, iron, cobalt, nickel, copper, zinc and cadmium has been investigated for their antibacterial activity and compared with clarithromycin by observin ...200216414867
antibacterial studies of cefixime copper, zinc and cadmium complexes.antibacterial studies of metal complexes of cefixime, with copper zinc and cadmium have been carried out by observing the minimum inhibitory concentration (mic) and by measuring the zone of inhibition of the complexes and compared with the parent cephalosporin against both gram negative and gram positive microorganisms. various microorganisms used were staphylococcus aureus, e. coli, klebsiella and proteus species. for mic observation, serial dilution method was employed and zone sizes were dete ...200216414863
resistance pattern of clinical isolates from cases of chronic ear infection ii.during present study hundred clinical isolates from cases of ear infection were tested from local population for resistance pattern, using eleven different antibiotics. a considerable percentage of isolates causing ear infection was resistant to number of antimicrobial drugs. the percentages of organisms isolated from ear infection are pseudomonas aeruginosa (50%), proteus species (16%), staphylococcus aureus (15%), klebsiella species (13%) and escherichia coli (6%).199816414816
phytochemical and antimicrobial studies of salvia splendens sello.the phytochemical investigation of antimicrobial agents from locally occurring salvia splendens sello, was carried out. three common solvents i.e. petroleum ether (40-60%), chloroform and methanol were successively used for the extraction of antimicrobial principles from its various parts. three major compounds (namely c1, c2, and c3) were isolated and purified from the active methanol extract of roots by column and thin layer chromatography. gram-positive bacteria (bacillus pumilus, b. subtilis ...199816414814
urinary tract infections associated with multidrug resistant enteric bacilli: characterization and genetical studies.urinary tract infections (utis) are among the most commonly prevalent infections in clinical practice. escherichia coli is the causative agent in about 85% of community-acquired utis, followed by klebsiella that accounts for 6 to 17% of such infections. present study is based on the isolation-identification and antibiotic resistance pattern of about 60 indigenous bacterial isolates from uti patients. prevalence rates were consistent with those from major recent studies reported in the literature ...200416414603
roxithromycin antagonism with essential and trace elements.in order to ascertain the role of various essential and trace element complexation on the antibacterial activity of various macrolide antibiotics, the synergistic or antagonistic behavior of roxithromycin metal complexes have been studied and compared with the parent drug. metal complexes of roxithromycin with magnesium, calcium, chromium, manganese, iron, cobalt, nickel, copper, zinc and cadmium have been investigated for their antibacterial activity and compared with roxithromycin by observing ...200416414588
serological classification and epitope specificity of proteus penneri s29 lipopolysaccharide.gram-negative bacteria of genus proteus are common human intestinal and urinary tract pathogens. in the genus proteus there are four clinically important named species: p. mirabilis, p. vulgaris, p. penneri, and p. hauseri, and three unnamed proteus genomospecies: 4, 5, and 6. the clinical significance of p. penneri, described in 1982 as a new species, is poorly documented. the aim of this work is serological characterization and classification of a ceftriaxone-susceptible p. penneri s29 strain ...200516407787
dissemination of proteus mirabilis isolates harboring ctx-m-14 and ctx-m-3 beta-lactamases at 2 hospitals in taiwan.from february to june 2003, 111 clinical isolates of proteus mirabilis were mainly isolated from patients with respiratory or urinary tract infections hospitalized at 3 district hospitals (a, b, c) in central taiwan. among them, 34 (30.6%) strains, isolated within 2 hospitals (a and b), exhibited nonsusceptibility to cefotaxime with significant reduction of mic (> or = 3 log2 dilution) by the effect of clavulanic acid, which confirmed the phenotype of extended-spectrum beta-lactamases (esbls). t ...200616406185
[problems of epidemic safety of drinking water use by the population of russia].quantitative relationships were studied between the indicators (common coliform bacteria (ccp), glucose-positive bacteria (gpb), thermoduric bacteria (tdb), coliform bacteria, enterococci, clostridia, coliphages) and the opportunistic (pseudomonas aeruginosa, proteus, klebsiella) and pathogenetic (salmonella and intestinal viruses) microorganisms at the stages of effluent purification and decontamination, in processes of self-purification in the water reservoirs and of water preparation at water ...200516404875
proteus syndrome with multiple genitourinary abnormalities.proteus syndrome is a rare hamartomatous disorder comprising a broad spectrum of congenital malformations and overgrowth of multiple tissues. some rare urogenital malformations have been reported before. this case is unique for the presence of multiple genitourinary anomalies including retroperitoneal cystic mass, intra-abdominal testicle with hematoma and ureterovesical stricture apart from common clinic findings of proteus syndrome.200616401930
proteus syndrome.proteus syndrome is a variable and complex disorder characterized by multifocal overgrowths affecting any tissue or structure of the body. we present a girl aged 3 years and 8 months with an epidermal nevus, port-wine stain, macrodactyly with gigantism of the feet, lymphohemagiomas and multiple lipomas.200516394465
unilateral proteus syndrome.proteus syndrome is a complex developmental abnormality. it is characterized by both hypertrophic and hypoplastic changes. deformities have been occasionally found to be localized in one half of the body in head or digit but presence of all signs in one half of the body in a wide spread manner is not reported in the literature. we report the case for its unusual presentation of unilateral localization of signs.200516394389
acute uncomplicated lower urinary tract infections in general practice: clinical and microbiological cure rates after three- versus five-day treatment with trimethoprim.epidemiological studies indicate that acute uncomplicated urinary tract infections (uti) in women can be successfully treated with short treatment regimens. however, the findings from the literature do not match experiences in daily practice.200516392777
antimicrobial activities of silver dressings: an in vitro comparison.a range of silver-coated or -impregnated dressings are now commercially available for use but comparative data on their antimicrobial efficacies are limited. the antibacterial activities of five commercially available silver-coated/impregnated dressings were compared against nine common burn-wound pathogens, namely methicillin-sensitive and -resistant staphylococcus aureus (mrsa), enterococcus faecalis, pseudomonas aeruginosa, escherichia coli, enterobacter cloacae, proteus vulgaris, acinetobact ...200616388031
bacteria isolated from the different developmental stages and larval organs of the obligate parasitic fly, wohlfahrtia magnifica (diptera: sarcophagidae).wohlfahrtia magnifica (diptera: sarcophagidae) is the major myiasis-causing fly species in the whole of eurasia for most important domestic animals. the aim of the present work was to obtain data on the culturable bacteria isolated under aerobic conditions from this fly: bacteria were isolated from all developmental stages (larvae, pupa, and imago) of wohlfahrtia magnifica, and the third-stage larval organs were also sampled. to determine the possible antagonistic effects between the dominant ba ...200616382282
update on the molecular genetics of vascular anomalies.genetic factors play a critical role in the pathogenesis of vascular anomalies. significant advances have been made in recent years in identifying the genetic and molecular determinants of a variety of vascular anomalies using a molecular genetic approach. several genes for vascular anomalies have been identified. these genes include aggf1 for klippel-trenaunay syndrome, rasa1 for capillary malformations, krit1, mgc4607, pdcd10 for cerebral cavernous malformations, glomulin for glomuvenous malfo ...200516379592
novel plasmid-mediated 16s rrna methylase, rmtc, found in a proteus mirabilis isolate demonstrating extraordinary high-level resistance against various aminoglycosides.proteus mirabilis ars68, which demonstrated a very high level of resistance to various aminoglycosides, was isolated in 2003 from an inpatient in japan. the aminoglycoside resistance of this strain could not be transferred to recipient strains escherichia coli csh-2 and e. coli hb101 by a general conjugation experiment, but e. coli dh5alpha was successfully transformed by electroporation with the plasmid of the parent strain, ars68, and acquired an unusually high degree of resistance against ami ...200616377684
purification, properties and extended solution structure of the complex formed between human immunoglobulin a1 and human serum albumin by scattering and ultracentrifugation.immunoglobulin a (iga) is unique amongst antibodies in being able to form polymeric structures that may possess important functions in the pathology of specific diseases. iga also forms complexes with other plasma proteins, the iga1-human serum albumin (hsa) complex (iga1-hsa) being typical. we have purified this complex using a novel two-step purification based on thiophilic chromatography and gel filtration, and characterised this. hsa is linked covalently to the tailpiece of iga1 by a disulph ...200616376934
antimicrobial activity of flavonoids from pelargonium radula (cav.) l'hérit.flavonoids from pelargonium radula (cav.) l'hérit were purified by column chromatography. two fractions were obtained: f1 (main flavonoid isoquercitrin) and f2 (main flavonoid rutin). in vitro antimicrobial activity of f1 and f2 were tested against eleven species of bacteria and eleven species of fungi. both fractions demonstrated strong inhibitory activity against staphylococcus aureus, proteus rettgeri, candida tropicalis and microsporum gypseum. staphylococcus sp. (coagulase-negative) and can ...200516375833
quantitative bacterial micro-assay for rapid detection of serum phenylalanine on dry blood-spots: application in phenylketonuria screening.phenylketonuria is an inherited metabolic disease, which is characterized by an increased level of serum phenylalanine. quantitative measurement of phenylalanine in the serum of phenylketonuria patients is necessary to confirm the disease, and to distinguish phenylketonuria from other forms of hyperphenylalaninemia. in this study, we report a rapid and inexpensive micro-assay for simultaneous detection and quantitative measurement of serum phenylalanine on dry blood-spots. analysis of the standa ...200616375590
susceptibility of recent bacterial isolates to cefdinir and selected antibiotics among children with urinary tract infections.cefdinir, an extended-spectrum cephalosporin administered orally, is approved by the u.s. federal drug administration for treatment of skin and respiratory tract infections. during the last two years at the authors' institution, this agent has been used as an off-label treatment for urinary tract infections in children.200616365328
pharmacologic characteristics of prulifloxacin.prulifloxacin, the prodrug of ulifloxacin, is a broad-spectrum oral fluoroquinolone antibacterial agent. after absorption, prulifloxacin is metabolized by esterases to ulifloxacin. the drug has a long elimination half-life, allowing once-daily administration. in vitro, ulifloxacin is generally more active than other fluoroquinolones against a variety of clinical isolates of gram-negative bacteria, including community and nosocomial isolates of escherichia coli, klebsiella spp., proteus, providen ...200616360331
neurocutaneous syndromes and epilepsy-issues in diagnosis and management.epilepsy may be seen as a feature of many of the neurocutaneous syndromes. the challenge lies within the diagnosis of the specific disorder and ultimately control of the epilepsy. tuberous sclerosis is the most common of the disorders with a frequency of 4.9/100,000. an autosomal-dominant condition, diagnostic features may be unclear under 2 years of age. population studies suggest a prevalence of epilepsy of 78%, the majority presenting under the age of 12 months, with a high association betwee ...200516359466
ctx-m-1 extended-spectrum beta-lactamase-producing proteus mirabilis in greece.to examine the dissemination of ctx-m-type extended-spectrum beta-lactamases (esbls) in clinical isolates of proteus mirabilis, 91 nonrepetitive esbl-producing p. mirabilis were collected from infected patients in a tertiary greek hospital during september, 2001, to may, 2004. a bla (ctx-m) gene was amplified in one isolate (strain a328), but bla (ctx-m) was not detected in any of the remaining esbl producers. sequencing results showed that p. mirabilis a328 produced a ctx-m-1 enzyme while pcr m ...200516359194
in vitro activities of tigecycline, ertapenem, isepamicin, and other antimicrobial agents against clinically isolated organisms in taiwan.this study evaluated the in vitro activities of tigecycline, ertapenem, isepamicin, and other comparators against 861 bacterial isolates recovered from patients treated in three major teaching hospitals in 2003. mics to antimicrobial agents were determined by the agar dilution method. high rates of oxacillin resistance (58%) in staphylococcus aureus (60 isolates), and vancomycin resistance (21%) and quinupristin-dalfopristin non-susceptibility (39%) in enterococcus faecium (34 isolates) were fou ...200516359192
determination of the antimicrobial properties of oligo-2-hydroxy-1-naphthaldehyde.oligo-2-hydroxy-1-naphthaldehyde (ohna) was synthesized by oxidative polycondensation using h2o2 (35%, aqueous solution), air o2 and naocl (34%, aqueous solution) by kaya and senol and the products were characterized by spectral techniques. antimicrobial activities of the first and second fractions of ohna were tested against corynobacterium xerosis ccm 2824, proteus vulgaris atcc 6897, staphylococcus epidermidis nrrl b-4877, s. aureus atcc 6538, enterobacter aerogenes atcc 13048, salmonella thy ...200516355978
proteus syndrome with giant myelolipoma in the pelvis.proteus syndrome is a very rare and complex disorder with malformations and overgrowth of multiple tissues. this disorder was designated proteus syndrome by wiedemann et al. to denote its variable clinical expression. our patient presented with macrodactyly, cerebriform appearance and a huge abdominal mass. a biopsy under laparotomy was performed, and histopathological examination revealed myelolipoma. tumor resection was performed. to our knowledge, no case of proteus syndrome presenting a myel ...200516351661
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