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modulation of crystalline proteus mirabilis biofilm development on urinary catheters.the crystalline biofilms formed by proteus mirabilis can seriously complicate the care of patients undergoing long-term bladder catheterization. the generation of alkaline urine by the bacterial urease causes calcium and magnesium phosphates to precipitate from urine and accumulate in the catheter biofilm, blocking the flow of urine from the bladder. the ph at which these salts crystallize from a urine sample, the nucleation ph (ph(n)), can be elevated by diluting the urine and by increasing its ...200616585633
the effect of hydrogen-ion concentration on locomotion and other life-processes in amoeba proteus. 192616587084
antibiotic-lock therapy for long-term intravascular catheter-related bacteraemia: results of an open, non-comparative study.infection is one of the main problems associated with long-term central venous catheters. this study assesses the effectiveness of antibiotic-lock therapy (alt) for treating catheter-related bacteraemia (crb).200616597634
a sensor to detect the early stages in the development of crystalline proteus mirabilis biofilm on indwelling bladder catheters.a simple sensor has been developed to detect the early stages of urinary catheter encrustation and avoid the clinical crises induced by catheter blockage. in laboratory models of colonization by proteus mirabilis, the sensor signaled encrustation at an average time of 43 h before catheters were blocked with crystalline biofilm.200616597888
bacteriological urinalysis in patients after renal transplantation.the study consisted of microbiological urinalysis performed in 269 patients after renal transplantation who remained under medical care at the outpatient service of the transplantation institute in warsaw. the patients enrolled into the study had undergone renal transplantation 6 to 72 months before urine samples were collected. 304 urinalysis were performed. in the group of 269 patients, 42 individuals had bacteria in their urine what was confirmed in 47 urine cultures. cases of bacteriuria wer ...200516599304
antimicrobial, wound healing and antioxidant activity of plagiochasma appendiculatum lehm. et lind.plagiochasma appendiculatum (aytoniaceae) of the order marchantiales is widely used in the form of paste ethnomedicinally by gaddi tribe in kangra valley for treating skin diseases. in this context, antimicrobical potential of plagiochasma appendiculatum against a wide range of microorganisms was studied. to validate the ethnotherapeutic claims of the plant in skin diseases, wound healing activity was studied, besides antioxidant activity to understand the mechanism of wound healing activity. th ...200616600543
purification of antibacterial agents from tragia involucrata--a popular tribal medicine for wound healing.tragia involucrata has been widely used in traditional systems of medicine for a variety of diseases. in the present study, in vitro antibacterial properties of nine different compounds including vinyl hexylether, shellsol, 2,4-dimethyl hexane, 2-methylnonane and 2,6-dimethyl heptane were isolated from the leaf of tragia involucrata studied against escherichia coli, proteus vulgaris and staphylococcus aureus using the disc-diffusion method at 50 microg/ml concentrations. the compound vinyl hexyl ...200616600546
universal primers for detection of common bacterial pathogens causing prosthetic joint infection.the diagnosis of low grade prosthetic joint infection is difficult and time consuming. nested-pcr for universal bacterial dna segments detection of "orthopaedic" bacteria was tested in a laboratory setting. this method is based on amplification of the 16s bacterial ribosomal rna coding sequences. 11 species of the most frequent bacterial pathogens (staphylococcus aureus, staphylococcus epidermidis, streptococcus pyogenes, streptococcus agalactiae, enterococcus faecium, enterococcus faecalis, kle ...200516601773
rheumatoid arthritis is an autoimmune disease triggered by proteus urinary tract infection.rheumatoid arthritis (ra) is a chronic and disabling polyarthritic disease, which affects mainly women in middle and old age. extensive evidence based on the results of various microbial, immunological and molecular studies from different parts of the world, shows that a strong link exists between proteus mirabilis microbes and ra. we propose that sub-clinical proteus urinary tract infections are the main triggering factors and that the presence of molecular mimicry and cross-reactivity between ...200616603443
[a 56-year-old woman with pain and crepitations in the left lower limb]. 200616606564
cold adapted features of vibrio salmonicida catalase: characterisation and comparison to the mesophilic counterpart from proteus mirabilis.the gene encoding catalase from the psychrophilic marine bacterium vibrio salmonicida lfi1238 was identified, cloned and expressed in the catalase-deficient escherichia coli um2. recombinant catalase from v. salmonicida (vsc) was purified to apparent homogeneity as a tetramer with a molecular mass of 235 kda. vsc contained 67% heme b and 25% protoporphyrin ix. vsc was able to bind nadph, react with cyanide and form compounds i and ii as other monofunctional small subunit heme catalases. amino ac ...200616609813
determination of usnic acid in some rhizoplaca species from middle anatolia and their antimicrobial activities.three species of lichens, rhizoplaca chrysoleuca (sm.) zopf, rhizoplaca melanopthalma (dc.) leuckert & poelt and rhizoplaca peltata ramonds leuckert, were collected from middle anatolia, erciyes mountain (kayseri) in turkey. their usnic acid amounts were determined by hplc in acetone extracts. in addition, antimicrobial activities of these extracts were determined against escherichia coli (atcc 35218), enterococcus faecalis (rskk 508), proteus mirabilis (pasteur ens. 235), staphylococcus aureus, ...200616610216
ciprofloxacin crystal nephropathy--a 'new' cause of acute renal failure. 200616611679
use of chitosan bandage to prevent fatal infections developing from highly contaminated wounds in mice.hemcon bandage is an engineered chitosan acetate preparation used as a hemostatic control dressing, and its chemical structure suggests that it should also be antimicrobial. we tested its ability to rapidly kill bacteria in vitro and in mouse models of infected wounds. we used the gram-negative species pseudomonas aeruginosa and proteus mirabilis and the gram-positive staphylococcus aureus that had all been stably transduced with the entire bacterial lux operon to allow in vivo bioluminescence i ...200616616364
synthesis and evaluation of antimicrobial and anticonvulsant activities of some new 3-[2- (5-aryl-1,3,4-oxadiazol-2-yl/4-carbethoxymethylthiazol-2-yl) imino-4-thiazolidinon-5-ylidene]-5-substituted/nonsubstituted 1h-indole-2-ones and investigation of their structure-activity relationships.in the present study, 20 new compounds having 3-[2-(5-aryl-1,3,4-oxadiazol-2-yl) imino-4-thiazolidinon-5-ylidene]-5-substituted/nonsubstituted 1h-indole-2-one (i-xii) and 3-[2-(4-carbethoxymethylthiazol-2-yl)imino-4-thiazoldinon-5-ylidenel-5-substituted/nonsubstituted ih-indole-2-one (xiii-xx) systems were synthesized. the structures were confirmed by spectral methods (uv, ir, 1h-nmr, 13c-nmr, 13c-dept (135), electron impact mass spectrometry) and elemental analysis. all compounds were tested fo ...200616618017
comparison of fecal microflora in children with atopic eczema/dermatitis syndrome according to ige sensitization to food.atopic eczema/dermatitis syndrome (aeds) commonly often arises during early infancy. in several intervention studies a beneficial influence on aeds course of certain intestinal bacteria, administered as 'probiotics', has been described. to evaluate the possible role of the natural intestinal microflora in children with allergic eczema/dermatitis syndrome regarding immediate type hypersensitivity to food allergens, children with food allergy (aaeds, n = 68) have been compared with children withou ...200616618364
[brain abscesses in neonates: neurosonographic diagnosis and long-term follow-up].brain abscesses were neurosongraphically diagnosed in 3 out of 44 neonates who had confirmed purulent meningitis. in two cases, the cause was proteus mirabillis, whereas in one the cause could not be isolated. the ultrasound finding indicated abscess cavities localised in the frontal (in one case bilaterally) and temporal regions of the cns. neurosurgical interventions were carried out on all of the neonates who had abscesses (including the evacuation of purulent cavity contents, and later on a ...200516623257
the anti-microbial activity of electrolysed oxidizing water against microorganisms relevant in veterinary medicine.standards of the german association of veterinary medicine (dvg) for the evaluation of chemical disinfectants were used to assess the anti-microbial efficacy of electrolysed oxidizing water (eow). enterococcus faecium, mycobacterium avium subspecies avium, proteus mirabilis, pseudomonas aeruginosa, staphylococcus aureus and candida albicans were exposed to anode eow (ph, 3.0+/-0.1; oxidation-reduction potential (orp), +1100+/-50 mv; free chlorine, 400+/-20 mg/l cl2) and combined eow (7:3 anode:c ...200616629725
observations on the adherence of proteus mirabilis onto polymer surfaces.infection of the catheterized urinary tract with proteus mirabilis causes blockage of the catheter by crystalline bacterial biofilms. the aim of this work is to identify a surface-coating for catheters that is not vulnerable to colonization by pr. mirabilis.200616630003
lipid-specific membrane activity of human beta-defensin-3.defensins represent a major component of innate host defense against bacteria, fungi, and enveloped viruses. one potent defensin found, e.g., in epithelia, is the polycationic human beta-defensin-3 (hbd3). we investigated the role of the lipid matrix composition, and in particular the presence of negatively charged lipopolysaccharides (lps) from sensitive (escherichia coli, salmonella enterica serovar minnesota) or resistant (proteus mirabilis) gram-negative bacteria or of the zwitterionic phosp ...200616634647
[sensitivity and resistance of aerobic bacteria isolated from patients with periodontitis towards antibiotics and bacteriophages (comparative analysis)].in order to examine sensitivity and resistance of isolated aerobic bacteria from periodontitis materials towards antibiotics and bacteriophages, there has been studied exudations taken from 737 patients' periodontic pockets or the tissue taken from curettage. according to the rate of identified microorganisms, they have been arranged as follows: s. epidermidis 39,34+/-1,56%; s. pyogenes 18,84+/-1,25%; m. catarrhalis 17,09+/-1,2%; s. aureus 10,71+/-0,99%; e.coli-5,66+/-0,74%; diphtheroids in 1,13 ...200616636376
pathogenic bacteria carried by companion animals and their susceptibility to antibacterial agents.results of the investigation showed that there was a difference in the bacteria isolated from dogs, cats and their living environment. the number and species isolated from the hair and front paw samples from dogs kept outdoors and from cats were greater and more varied than those from the samples from dogs kept indoors. staphylococcus, micrococcus and bacillus were frequently detected from skin surfaces. on the other hand, escherichia, pseudomonas, proteus and others were detected on each sampli ...200616637433
comparative evaluation of bact/alert 3d and bactec systems for the recovery of pathogens causing bloodstream infections.to compare bact/alert (bta) and bactec 9240 (bac), two continuously monitoring automated blood culture systems, for the recovery of bloodstream pathogens and standard media available for these systems.200616651840
trends in production of extended-spectrum beta-lactamases among enterobacteria of medical interest: report of the second italian nationwide survey.results of a 2003 survey carried out in italy to evaluate the prevalence of extended-spectrum beta-lactamase (esbl)-producing enterobacteria are presented. eleven italian microbiology laboratories investigated 9,076 consecutive nonreplicate isolates (inpatients, 6,850; outpatients, 2,226). esbl screening was performed by mic data analysis. confirmation was obtained using the double-disk synergy test and the combination disk test based on clsi methodology. esbl determinants were investigated by c ...200616672390
[mutations of dna gyrase and topoisomerase iv in clinical isolates of fluoroquinolone-resistant proteus mirabilis].the presence of fluoroquinolone resistance-associated mutations within the quinolone resistance-determining region of dna gyrase and topoisomerase iv was investigated genetically in clinical isolates of proteus mirabilis recovered from patients with urinay tract infections. two isolates of fluoroquinolone-resistant p. mirabilis possessed the mutations in gyra (ser-83 --> arg or ile), gyrb (ser-464 --> tyr or phe) and parc (ser-80 --> ile). a novel mutation with glu-87 --> lys in gyra, where sugg ...200616673582
doppler flow imaging of cytoplasmic streaming using spectral domain phase microscopy.spectral domain phase microscopy (sdpm) is a function extension of spectral domain optical coherence tomography. sdpm achieves exquisite levels of phase stability by employing common-path interferometry. we discuss the theory and limitations of doppler flow imaging using sdpm, demonstrate monitoring the thermal contraction of a glass sample with nanometer per second velocity sensitivity, and apply this technique to measurement of cytoplasmic streaming in an amoeba proteus pseudopod. we observe r ...200616674204
performance in detection and reporting beta-lactam resistance phenotypes in enterobacteriaceae: a nationwide proficiency study in italian laboratories.we evaluated the ability of 60 italian clinical microbiology laboratories in detecting and reporting beta-lactam resistance phenotypes in enterobacteriaceae. laboratories received 5 well-characterized isolates producing extended-spectrum beta-lactamases (esbls), 2 hyperproducers of chromosomal enzymes, and 3 quality control strains. the performances in antimicrobial susceptibility testing (ast) were different depending on the species and type of esbl produced. high rates of very major errors (up ...200616678993
antimicrobial activity of 1,2-bis-[2-(5-r)-1h-benzimidazolyl]- 1,2-ethanediols, 1,4-bis-[2-(5-r)-1h-benzimidazolyl]- 1,2,3,4-butanetetraols and their feiii, cuii, and agi complexes.1,2-bis-[2-(5-h/me/cl/no2)-1h-benzimidazolyl]-1,2-ethanediols (l1-l4), 1,4-bis-[2-(5-h/me/cl)-1h-benzimidazolyl]-1,2,3,4-butanetetraols (l5-l7) and their complexes with fecl3, cucl2, and agno3 were synthesized; antibacterial activity of the compounds was determined toward staphylococcus aureus, staphylococcus epidermidis, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, salmonella typhi, shigella flexneri, proteus mirabilis, and antifungal activity against candida albicans. the a ...200516681141
antimicrobial activity of znii, cdii, and hgii complexes of 1,2-bis-[2-(5-r)-1h-benzimidazolyl]-1,2-ethanediols and 1,4-bis-[2-(5-r)-1h-benzimidazolyl]-1,2,3,4-butanetetraols.1,2-bis-[2-(5-h/me/cl/no2)-1h-benzimidazolyl]-1,2-ethanediols (l1-l4), 1,4-bis-[2-(5-h/me/cl)-1h-benzimidazolyl]-1,2,3,4-butanetetraols (l5-l7) and their complexes with zncl2, cdcl2 and hgcl2 were synthesized and antibacterial activity of the compounds was tested toward staphylococcus aureus, s. epidermidis, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, salmonella typhi, shigella flexneri, proteus mirabilis and antifungal activity against candida albicans. hgii complexes have ...200516681142
[patterns of susceptibility to antibiotics of enterobacteriaceae causing intra-abdominal infection in spain: smart 2003 study outcomes].smart (study for monitoring antimicrobial resistance trends) is an ongoing global antimicrobial surveillance program focused on clinical isolates from intra-abdominal infections. the objective of this subanalysis was to assess antimicrobial susceptibility patterns among entero-bacteriaceae recovered at 13 participating spanish sites during 2003. antimicrobial susceptibility testing was performed using broth microdilution techniques according to the clsi (formerly nccls) guidelines for mic testin ...200616688292
antibacterial and antioxidant activities in sideritis italica (miller) greuter et burdet essential oils.sideritis italica (miller) greuter et burdet is a widespread lamiacea in the mediterranean region used in traditional medicine. essential oils were antibacterial against nine atcc and as many clinically isolated gram-positive and gram-negative bacterial strains. antibacterial activity was also found against helicobacter pylori: a dose-dependant inhibition was shown between 5 and 25 microg/ml. the antibacterial activity of the oils was expressed as mics (minimum inhibitory concentrations) and mbc ...200616690235
otogenic cerebellar abscess: an unusual occurrence. 200616696351
triclosan loaded ureteral stents decrease proteus mirabilis 296 infection in a rabbit urinary tract infection model.infection and encrustation remain major limitations of ureteral stent use and to our knowledge no device has completely overcome these obstacles to date. triclosan is a biocide currently used in a plethora of consumer and medical products that has recently been loaded into a ureteral stent. using a rabbit model of uti we examined the effects of triclosan impregnated stent segments on the growth and survival of proteus mirabilis, a uropathogen commonly associated with device related uti and encru ...200616697868
structure of the o-polysaccharide of proteus mirabilis ccug 10705 (of) containing an amide of d-galacturonic acid with l-alanine.the structure of the o-polysaccharide of proteus mirabilis ccug 10705 (of) was determined by chemical analyses along with one- and two-dimensional (1)h and (13)c nmr spectroscopy. the polysaccharide was found to contain an amide of d-galacturonic acid with l-alanine and based on the uniqueness of the o-polysaccharide structure and serological data, it was suggested to classify p. mirabilis of into a new separate proteus serogroup, o74. a weak cross-reactivity of p. mirabilis of and p. mirabilis ...200616697996
gram-negative meningitis and infections in individuals treated with intrathecal baclofen for spasticity: a retrospective study.the aim of this retrospective study was to describe signs, symptoms, and clinical outcomes of individuals undergoing intrathecal baclofen (itb) therapy who experienced pump-related gram-negative infections including meningitis. participants included 12 individuals (nine males, three females) aged 10 to 32 years (mean 17y 9mo), nine of whom had quadriplegic cp. a total of 571 baclofen pump surgeries were performed with 45 total infections. of the 45 infections, 12 were by gram-negative organisms, ...200616700935
epidermal naevus in proteus syndrome showing loss of heterozygosity for an inherited pten mutation.a 3-year-old boy with proteus syndrome has a novel germline p.y68d mutation of the pten gene inherited from his mother who has cowden syndrome. in addition, dna extracted from curettings of his widespread epidermal naevus shows loss of heterozygosity for this mutation. to our knowledge, this has not been described before.200616704655
[alkaline phosphatase in amoeba proteus].in free-living amoeba proteus (strain b), 3 phosphatase were found after disc-electrophoresis of 10 microg of protein in page and using 1-naphthyl phosphate as a substrate a ph 9.0. these phosphatases differed in their electrophoretic mobilities - "slow" (1-3 bands), "middle" (one band) and "fast" (one band). in addition to 1-naphthyl phosphate, "slow" phosphatases were able to hydrolyse 2-naphthyl phosphate and p-nitrophenyl phosphate. they were slightly activated by mg2+, completely inhibited ...200516706160
antimicrobial activities of cinnamon oil and cinnamaldehyde from the chinese medicinal herb cinnamomum cassia blume.both cinnamomum verum j.s. presl. and cinnamomum cassia blume are collectively called cortex cinnamonmi for their medicinal cinnamon bark. cinnamomum verum is more popular elsewhere in the world, whereas c. cassia is a well known traditional chinese medicine. an analysis of hydro-distilled chinese cinnamon oil and pure cinnamaldehyde by gas chromatography/mass spectrometry revealed that cinnamaldehyde is the major component comprising 85% in the essential oil and the purity of cinnamaldehyde in ...200616710900
using interrupted time series analysis to assess associations of fluoroquinolone formulary changes with susceptibility of gram-negative pathogens and isolation rates of methicillin-resistant staphylococcus aureus.the use of fluoroquinolones has been linked to increasing bacterial resistance and infection and/or colonization with already resistant pathogens both as a risk factor and based on volume of use. changes in individual fluoroquinolones used in an institution may also be related to these clinical problems. interrupted time series analysis, which allows for assessment of the associations of an outcome attributable to a specific event in time, was used to study the effect of changes in our hospital' ...200616723572
postpartum uterine flora following normal and abnormal puerperium in cows.uterine swabs were taken from the uteri of three groups of cows (n = 5 each) on days 1 and 2 and then at 3-d intervals up to 32 d post partum. group i cows had a normal delivery, group ii cows had dexamethasone-induced parturition, and group iii cows were subjected to a caesarean section. all cows in group iii and two cows in group ii retained their fetal membranes. a total of 230 bacterial isolates were made, and the most frequently isolated bacteria were escherichia coli, streptococci, proteus ...199016726838
antimicrobial activity of caesalpinia pulcherrima, euphorbia hirta and asystasia gangeticum.the ethanolic extracts of the dry fruits of caesalpinia pulcherrima, aerial parts of euphorbia hirta and flowers of asystasia gangeticum were tested for antimicrobial activity. the three plants exhibited a broad spectrum of antimicrobial activity, particularly against escherichia coli (enteropathogen), proteus vulgaris, pseudomonas aeruginosa and staphylococcus aureus.200616730921
characterization and serological classification of o-specific polysaccharide of proteus mirabilistg 276-90 from proteus serogroup o34.gram-negative bacteria of the genus proteus from the family enterobacteriaceae are currently divided into the five species p. mirabilis, p. vulgaris, p. penneri, p. hauseri, and p. myxofaciens and three unnamed proteus genomospecies 4, 5, and 6. they are important facultative human and animal pathogens which, under favorable conditions, cause mainly intestinal and urinary tract infections, sometimes leading to serious complications such as acute or chronic pyelonephritis and the formation of bla ...200616736109
in vitro activity of an ear rinse containing tromethamine, edta, and benzyl alcohol on bacterial pathogens from dogs with otitis.to evaluate the in vitro activity of an ear rinse (er) containing tromethamine, edta, and benzyl alcohol on bacterial pathogens from dogs with otitis.200616740099
case report: acute unintentional carbachol intoxication.intoxications with carbachol, a muscarinic cholinergic receptor agonist are rare. we report an interesting case investigating a (near) fatal poisoning.200616740173
the anaerobic decomposition of l-cystine by washed cells of proteus vulgaris. 193316745154
the effect of carbohydrates and allied substances on urease production by proteus vulgaris. 193716746341
production of amines by bacteria: the decarboxylation of amino-acids by organisms of the groups clostridium and proteus with an addendum by g. l. brown, f. c. macintosh and p. bruce white. 194116747390
the use of micro-organisms in sugar analysis: the effects of various salts on the activity of proteus vulgaris in removing glucose and some possible sources of error in its use as a reagent for the estimation of glucose. 194116747440
effectiveness of flouroquinolone therapy for gram-negative infections within the intensive care unit setting. 200616750952
antimicrobial and toxicological profile of the new biocide akacid plus.akacid plus is a new member of the polymeric guanidine family of disinfectants. it was especially developed to enhance the antimicrobial activity of this class with significantly less toxicity. the in vitro activity of akacid plus compared with chlorhexidine digluconate and mupirocin was tested against a total of 369 recent clinical isolates.200616751199
[proteus mirabilis community-acquired pneumonia]. 200616752451
[prevalence of esbl strains in urinary tract infections in children in 1996 and 2004].ethiological agents in urinary tract infections and prevalence of bacterial strains producing extended spectrum of beta-lactamases isolated from urine of children with urinary tract infections were compared. patients were hospitalized in department of pediatry, pediatric gastroenterology and oncology of medical university of gdaƄsk in 1996 and 2004. seventy bacterial strains isolated from urine in 1996 and 113 strains isolated in 2004 were analyzed. in both years enterobacteriaceae were the most ...200616758740
[a young patient with portal and mesenteric vein thrombosis and proteus syndrome].in the 26th year of life a young woman suffered a portal and mesenteric thrombosis followed by portal hypertension with splenomegaly, esophageal varices and pancytopenia. after splenorenal shunt surgery and splenectomy hematologic parameters resolved rapidly. also, she was suffering of proteus syndrome, which is an extremely rare and sporadic hamartomatous disorder characterized by a variety of cutaneous and subcutaneous tumors including vascular malformations, several types of nevi, partial gig ...200616758842
three cases of xanthogranulomatous epididymitis caused by e. coli.xanthogranulomatous epididymitis is an uncommon but benign process, characterized by tissue destruction and the accumulation of abundant foamy macrophages mixed with lymphocytes and plasma cells. the pathogens generally found to cause xanthogranulomatous inflammation have been proteus and e. coli. we identified such a pathogen by sequencing divergent regions of 16s rrna, followed by pathogen-specific-pcr using alr, ipah, ial, and iuc primer pairs in dna microdissected from an h&e stained slide. ...200716762415
structure and evolution of the mitochondrial control region and flanking sequences in the european cave salamander proteus anguinus.the european cave salamander proteus anguinus laurenti 1768 is one of the best-known subterranean animals, yet its evolutionary history and systematic relationships remain enigmatic. this is the first comprehensive study on molecular evolution within the taxon, using an mtdna segment containing the control region (cr) and adjacent sequences. two to seven tandem repeats of 24-32 bp were found in the intergenic spacer region (vntr1), and three, four or six repeats, 59-77 bp each, in the 3' end of ...200616764998
emergence of proteus mirabilis carrying the bla metallo-beta-lactamase gene.seven genetically related proteus mirabilis clinical isolates from a hospital in thessaloniki, greece, exhibited decreased susceptibility to imipenem and carried a bla(vim-1) metallo-beta-lactamase gene. pcr mapping revealed that bla(vim-1) was part of a class 1 integron that was probably located in the chromosome and also included the aaca7, dhfr and aada genes. this is the first description of the bla(vim-1) metallo-beta-lactamase gene in p. mirabilis.200616774572
improving the accuracy of protein secondary structure prediction using structural alignment.the accuracy of protein secondary structure prediction has steadily improved over the past 30 years. now many secondary structure prediction methods routinely achieve an accuracy (q3) of about 75%. we believe this accuracy could be further improved by including structure (as opposed to sequence) database comparisons as part of the prediction process. indeed, given the large size of the protein data bank (>35,000 sequences), the probability of a newly identified sequence having a structural homol ...200616774686
biofilm formation in an ice cream plant.the sites of biofilm formation in an ice cream plant were investigated by sampling both the production line and the environment. experiments were carried out twice within a 20-day period. first, stainless steel coupons were fixed to surfaces adjacent to food contact surfaces, the floor drains and the doormat. they were taken for the analysis of biofilm at three different production stages. then, biofilm forming bacteria were enumerated and also presence of listeria monocytogenes was monitored. b ...200616779628
[extended-spectrum-beta-lactamase-producing proteus mirabilis: laboratory-based surveillance in cooperation with 12 clinical laboratories in the kinki region of japan].we studied 247 strains of proteus mirabilis collected during the 6 months from november 2003 to april 2004 from 12 clinical laboratories in the kinki region of japan for the production of extended-spectrum beta-lactamase (esbl). eighteen strains (7.3%) showed mics for cefpodoxime of > or = 2 microg/ml and 13 strains (5.2%) were positive for the double-disk synergy test. susceptibility depended on genotype. mics for cefepime, cefozopran, and cefpirome were high (> or = 8 microg/ml), and that for ...200616780129
synthesis and in vitro antimicrobial studies of medicinally important novel n-alkyl and n-sulfonyl derivatives of 1-[bis(4-fluorophenyl)-methyl]piperazine.a series of novel substituted 1-[bis(4-fluorophenyl)-methyl]piperazine derivatives (4a-g) and (5h-m) have been synthesized. the synthesized compounds were characterized by ir and 1h nmr. all the synthesized compounds were evaluated in vitro for their efficacy as antimicrobial agents against representative strains of gram-positive (staphylococcus aureus atcc 25953, streptococcus pneumoniae atcc 49619, bacillus cereus 11778, and bacillus subtilis 6051) and gram-negative bacteria (escherichia coli ...200616784863
the comparison of pyrosequencing molecular gram stain, culture, and conventional gram stain for diagnosing orthopaedic infections.we have developed a combined real-time pcr and pyrosequencing assay that successfully differentiated the vast majority of gram-positive and gram-negative bacteria when bacterial isolates were tested. the purpose of this study was to evaluate this assay on clinical specimens obtained from orthopedic surgeries, and to prospectively compare the results of "molecular gram stain" with culture and conventional direct gram stain. forty-five surgical specimens were obtained from patients who underwent o ...200616788984
tigecycline: a new glycylcycline antimicrobial agent.the pharmacology, spectrum of activity, pharmacokinetics, clinical efficacy, adverse events, dosage and administration, drug interactions, and place in therapy of tigecycline are reviewed.200616790575
structure of escherichia coli tryptophanase.pyridoxal 5'-phosphate (plp) dependent tryptophanase has been isolated from escherichia coli and its crystal structure has been determined. the structure shares the same fold with and has similar quaternary structure to proteus vulgaris tryptophanase and tyrosine-phenol lyase, but is found in a closed conformation when compared with these two enzymes. the tryptophanase structure, solved in its apo form, does not have covalent plp bound in the active site, but two sulfate ions. the sulfate ions o ...200616790938
capillary isoelectric focusing of microorganisms in the ph range 2-5 in a dynamically modified fs capillary with uv detection.the isoelectric points of many microbial cells lie within the ph range spanning from 1.5 to 4.5. in this work, we suggest a cief method for the separation of cells according to their isoelectric points in the ph range of 2-5. it includes the segmental injection of the sample pulse composed of the segment of the selected simple ampholytes, the segment of the bioanalytes and the segment of carrier ampholytes into fused silica capillaries dynamically modified by poly(ethylene glycole). this polymer ...200616791563
a simple phenotypic method for differentiation between acquired and chromosomal ampc beta-lactamases in escherichia coli.screening methods for the detection of plasmid-mediated ampc beta-lactamases are technically demanding. the purpose of this study was to assess screening methods for the detection of these enzymes in clinical isolates of escherichia coli, klebsiella pneumoniae and proteus mirabilis.200616792938
gram-negative bacteria from patients seeking medical advice in stockholm after the tsunami catastrophe.microbiological cultures from 229 patients seeking medical advice in stockholm after the tsunami catastrophe of december 2004 were analysed at the clinical microbiology laboratory, karolinska university hospital, stockholm, sweden. gram-negative bacilli were the most common findings from wound cultures. common human pathogens such as escherichia coli, proteus species, klebsiella spp., and pseudomonas aeruginosa were isolated. more rare species of gram-negative bacilli, e.g. myroides odoratus, sp ...200616798691
proteus mirabilis spondylodiscitis complicating a urinary tract infection.infectious spondylodiscitis results from local or haematogenous infection by microorganisms of intervertebral disc spaces, vertebral bodies and surrounding structures. haematogenous invasion may follow urosepsis. we report on a case of septic spondylodiscitis following urosepsis with proteus mirabilis, a frequently isolated microorganism in urinary tract infections but rarely in spondylodiscitis.200616798719
the ability of flagellum-specific proteus vulgaris bacteriophage pv22 to interact with campylobacter jejuni flagella in culture.there has been a recent resurgent interest in bacteriophage biology. research was initiated to examine campylobacter jejuni-specific bacteriophage in the russian federation to develop alternative control measures for this pathogen.200616803630
proteus mirabilis isolates of different origins do not show correlation with virulence attributes and can colonize the urinary tract of mice.proteus mirabilis has been described as an aetiological agent in a wide range of infections, playing an important role in urinary tract infections (utis). in this study, a collection of p. mirabilis isolates obtained from clinical and non-clinical sources was analysed in order to determine a possible correlation between origin, virulence factors and in vivo infectivity. isolates were characterized in vitro, assessing several virulence properties that had been previously associated with p. mirabi ...200616804188
mapping bacterial surface population physiology in real-time: infrared spectroscopy of proteus mirabilis swarm colonies.we mapped the space-time distribution of stationary and swarmer cells within a growing proteus mirabilis colony by infrared (ir) microspectroscopy. colony mapping was performed at different positions between the inoculum and the periphery with a discrete microscope-mounted ir sensor, while continuous monitoring at a fixed location over time used an optical fiber based ir-attenuated total reflection (atr) sensor, or "optrode." phenotypes within a single p. mirabilis population relied on identific ...200616808858
chloral hydrate plates for the inhibition of swarming of proteus. 194816810793
[colonization of cental venous catheter in the intensive care units at the institute of cardiovascular disease "dedinje"--one year study].the application of central venous catheters (cvc) is associated with increased risk of microbial colonization and infection. the aim of present study was to assess the frequency of pathogens colonizing cvc and to determine their susceptibility pattern to various antimicrobial agents.200516812991
colonization of sitka spruce stumps by decay-causing hymenomycetes in paired inoculations.sitka spruce stumps were inoculated with decay fungi using colonized sawdust or dowel inoculum to investigate colonization in paired combinations. estimates of domain sizes were made in the top 15cm of stump after 13-14 or 21-23 months with sawdust or dowel inoculations, respectively. none of the co-inoculated species prevented colonization by heterobasidion annosum; sapwood colonization by resinicium bicolor may limit growth of h. annosum colonies out of heartwood, reducing the incidence of dis ...200616814536
characterisation of prototype nurmi cultures using culture-based microbiological techniques and pcr-dgge.undefined nurmi-type cultures (ntcs) have been used successfully to prevent salmonella colonisation in poultry for decades. such cultures are derived from the caecal contents of specific-pathogen-free birds and are administered via drinking water or spray application onto eggs in the hatchery. these cultures consist of many non-culturable and obligately anaerobic bacteria. due to their undefined nature it is difficult to obtain approval from regulatory agencies to use these preparations as direc ...200616814892
in vitro activities of various piperacillin and sulbactam combinations against bacterial pathogens isolated from intensive care units in taiwan: smart 2004 programme data.we investigated the in vitro activity of various piperacillin and sulbactam combinations against gram-negative bacterial isolates from intensive care units (icus) in taiwan. antimicrobial susceptibility testing of 1030 bacterial isolates recovered from icus of nine major teaching hospitals was performed using the agar dilution method. sulbactam was added to piperacillin either at a fixed sulbactam concentration of 4 mg/l and 8 mg/l or at a piperacillin:sulbactam ratio of 2:1 and 4:1. piperacilli ...200716815690
nutritive value, antioxidant and antimicrobial properties of struchium sparganophora leaves.struchium sparganophora leaf is a green leafy vegetable popularly used in soup preparations in processed and unprocessed forms in nigeria. fresh leaves of s. sparganophora were soaked overnight to remove its bitterness, before determination of the nutrient and antinutrient content, while sun-dried s. sparganophora leaves were extracted with rectified spirit. the antioxidant and antimicrobial properties of the extracts were subsequently determined. the result of the study revealed that the fresh ...200616822216
pharmacokinetic and pharmacodynamic aspects of antimicrobial agents for the treatment of uncomplicated urinary tract infections.uncomplicated urinary tract infections (uti) are treated with beta-lactams, co-trimoxazole, quinolones and fosfomycin tromethamine. due to increasing resistance of causative pathogens, antibiotics should be used by considering their pharmacodynamic and pharmacokinetic characteristics. beta-lactams have time-dependent activity and should not be used once-daily. co-trimoxazole should be restricted due to increasing chemoresistance. fluoroquinolones play a primary role in the treatment of serious a ...200616829051
hospital acquired urinary tract infections in urology departments: pathogens, susceptibility and use of antibiotics. data from the pep and peap-studies.data from two internet-based studies on nauti in hospitalized urological patients are presented together: the pan european prevalence (pep) study, which was a 1-day prevalence study in november 2003; and the pan euro-asian prevalence (peap) study, which was carried out in november 2004. overall, 93 and 101 hospitals from the two studies, respectively, completed the hospital questionnaires and provided patient information for the present study. nauti was diagnosed according to the centres for dis ...200616829052
urinary tract infection caused by fluoroquinolone- and cephem-resistant enterobacteriaceae.in 2001, fluoroquinolone-resistant escherichia coli isolates emerged in japan in patients with uncomplicated urinary tract infection (uti), and accounted for ca. 8% of isolates. this is a worldwide occurrence as reported by the eco.sens study. the number of fluoroquinolone- and cephem-resistant enterobacteriaceae isolates from patients with complicated uti is increasing. most cephem-resistant isolates of e. coli, klebsiella pneumoniae and proteus mirabilis produce extended-spectrum beta-lactamas ...200616829056
[wound care management of ulceration in a palliative care patient].the wound care management of a palliative care patient with sinusitis pinoidalis is documented. the treatment is validated by the expert's opinion. there is only little evidence-based treatment of such a lesion. in our experience, photo documentation is helpful in decision-making during the treatment process. in addition, healing of larger defects seems to improve upon frequent and extensive liquid cleansing.200616830251
structure of the glycerol phosphate-containing o-polysaccharides and serological studies of the lipopolysaccharides of proteus mirabilis ccug 10704 (oe) and proteus vulgaris tg 103 classified into a new proteus serogroup, o54.o-polysaccharides were obtained from the lipopolysaccharides of proteus mirabilis ccug 10704 (oe) and proteus vulgaris tg 103 and studied by chemical analyses and one- and two-dimensional (1)h and (13)c nuclear magnetic resonance spectroscopy, including rotating-frame nuclear overhauser effect spectroscopy, h-detected (1)h,(13)c heteronuclear single-quantum spectroscopy and (1)h,(31)p heteronuclear multiple-quantum spectroscopy experiments. the proteus mirabilis oe polysaccharide was found to ha ...200616831214
[a puzzle of the physician and the patient]. 200616835961
[clinical effect of continuous infusion of meropenem on bacterial pneumonia in the elderly].we studied the clinical effect of continuous infusion over 24 hours of meropenem (mepm) on bacterial pneumonia in the elderly (over 65). the subjects were 26 patients (community-acquired pneumonia: moderate, n = 9; severe, n= 4; hospital-acquired pneumonia: group iii, n = 13) whose performance status was 3 or 4. mepm 1.0g/day was infused continuously for 7-14 days, and its clinical efficacy, bacteriological efficacy, and side effects were examined prospectively. it was effective in 23 of the 26 ...200616841713
the mechanism of plasmid curing in bacteria.bacterial plasmids have a major impact on metabolic function. lactose fermentation of e. coli or hemolysin b transporter expressed by the plasmids that carry these respective genes could be readily obviated by heterocyclic compounds that readily bind to plasmid dna. these compounds could also reverse the resistance to antibiotics of e. coli, enterobacter, proteus, staphylococcus and yersinia strains by eliminating plasmids. however, the frequency and extent of this effect was significantly less ...200616842214
[epidemiology and etiology of urinary tract infections in the community. antimicrobial susceptibility of the main pathogens and clinical significance of resistance].urinary tract infections (uti) are a frequent problem in primary care. they occur mainly in women without underlying diseases and with no functional or structural anomalies of the urinary tract; consequently most cases are considered uncomplicated uti. etiology is influenced by factors such as age, diabetes, spinal cord injury, urinary catheterization, and other factors. escherichia coli causes 80-85% of acute episodes of uncomplicated cystitis. staphylococcus saprophyticus, proteus mirabilis, s ...200516854352
[low-intensity laser radiation in preoperative preparation of patients with benign prostatic hyperplasia].low-intensity laser therapy administered in the form of intravenous blood irradiation, transrectal and transurethral prostatic irradiation and their combination as preoperative preparation and correction of immunity disturbances in patients with benign prostatic hyperplasia (bph) were studied. the response to the treatment was evaluated by positive changes in the immune status and bacterial contamination of the urine and prostatic tissue. conventional preoperative preparation (uroantiseptics, an ...200016856453
[case no. 6. primary abscess of the right psoas-iliac compartment caused by proteus mirabilis in a female patient with nf1]. 200616858877
cysteine based novel noncompetitive inhibitors of urease(s)--distinctive inhibition susceptibility of microbial and plant ureases.based on the catalysis mechanism of urease, a homologous series of 10 cysteine derivatives (cysds) was designed and synthesized, and their inhibitory activities were evaluated for microbial ureases (bacillus pasteurii, bpu, and proteus mirabilis, pmu) and for a plant urease [jack bean (cavavalia ensiformis), jbu]. as already described, thiol-compounds might inhibit urease activity by chelating the nickel atoms involved in the catalysis process. in contrast to cysteine, which has been reported to ...200616859909
structures and serology of the o-antigens of proteus strains classified into serogroup o17 and former serogroup o35.bacteria of the genus proteus are facultative pathogens which commonly cause urinary tract infections. based on the serological specificity of the o-chain polysaccharide of the lipopolysaccharide (o-polysaccharide, o-antigen), strains of p. mirabilis and p. vulgaris have been classified into 60 serogroups. studies on the chemical structure and serological specificity of the o-antigens aim at the elucidation of the molecular basis and improvement of the serological classification of these bacteri ...200616868723
antimicrobial activity of extracts of the lichen xanthoparmelia pokornyi and its gyrophoric and stenosporic acid constituents.the antimicrobial activity of the diethyl ether, acetone, chloroform, petroleum ether, and ethanol extracts of the lichen xanthoparmelia pokornyi and its gyrophoric acid and stenosporic acid constituents has been screened against some foodborne bacteria and fungi. both the extracts and the acids showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, proteus vulgaris, staphylococcus aureus, streptococcus faecalis, yersinia enterocol ...200616869486
role of type ii topoisomerase mutations and acrab efflux pump in fluoroquinolone-resistant clinical isolates of proteus mirabilis.we conducted a study to determine the role played by amino acid mutations in dna gyrase and topoisomerase iv, and the acrab efflux pump in resistance to fluoroquinolones in clinical isolates of proteus mirabilis.200616870650
systemic absorption of gentamicin nasal irrigations.to determine if gentamicin nasal irrigation is systemically absorbed, and to identify any ototoxic side effects related to its use.200616871924
hemihyperplasia syndromes.hemihyperplasia is a heterogenous group of disorders characterized by asymmetric limb growth. there is considerable confusion regarding their classification and ascertainment into various syndromes. we tried to look into the various aspects of hemihyperplasia syndromes.200616877856
sterilisation of lucilia cuprina wiedemann maggots used in therapy of intractable wounds.three new techniques of sterilising maggots of lucilia cuprina for the purpose of debriding intractable wounds were studied. these techniques were utilisation of ultra-violet c (uvc) and maggot sterilisation with disinfectants. the status of sterility was checked on nutrient agar and blood agar and confirmed with staining. the indicators for the effectiveness of the methods were sterility and survival rate of the eggs or larvae. egg sterilisation with uvc had the lowest hatching rate (16+/-0.00% ...200516883286
bacteria fauna from the house fly, musca domestica (l.).the house fly, musca domestica has long been considered a potential agent for disease transmission ever since its existence. the general truth of this assertion remains undisputed till the present day in spite of increasing awareness toward an improved sanitation and better hygiene. the habitual movement of house fly from filthy substrata such as human faeces, animal excreta, carcasses, garbage, etc. makes them ideal candidates for disease transmission such as cholera, shigellosis, salmonellosis ...200516883292
the challenges of proteus syndrome: diagnosis and management.proteus syndrome (ps) is a disorder of patchy or mosaic postnatal overgrowth of unknown etiology. the onset of overgrowth typically occurs in infancy and can involve any tissue of the body. commonly involved tissues include connective tissue and bone, skin, central nervous system, the eye, but it apparently can affect any tissue. diagnosing of ps is difficult and the diagnostic criteria are controversial. our group advocates stringent diagnostic criteria to facilitate research and appropriate cl ...200616883308
[acute abdomen secondary to spontaneous uterine rupture associated with pyometra].a 71-year-old female with rheumatoid arthritis and chronic use of corticosteroids presented to the emergency room with 2 weeks of urinary symptoms, abdominal pain and a mass located in hypo-mesogastrium and both flanks. an x-ray film of the abdomen showed that bowels were displaced by the mass. laboratory studies showed thrombocytosis (549,000/mm(3)) and leukocytosis (41,800/mm(3)). several hours after her arrival the patient developed acute abdomen and surgery was indicated. a urinary catheter ...200616887087
swarming characteristics of proteus mirabilis under anaerobic and aerobic conditions.nutrients have a pronounced effect on the growth and swarming behaviour of proteus mirabilis 7002. iron, zinc, amino acids, and dioxygen are important for rapid growth and normal swarming. anaerobically grown cultures of p. mirabilis 7002 were unable to swarm on anaerobically maintained rich nutrient agar. upon exposure to aerobic conditions, p. mirabilis 7002 resumed swarming behaviour. scanning electron microscopy was used to demonstrate the presence of community organization and mature rafts ...199516887546
[heat shock proteins of freshwater protists and their involvement in adaptation to changes in the environmental salinity].changes in the level of heat shock proteins (hsp) in cells of freshwater protists, amoebae amoeba proteus and ciliates paramecium jenningsi, in response to changes in the environmental salinity were investigated. changes in salinity levels were considered as a stress factor. the immunoblotting method revealed a polypeptide antigen cross-reacting with antibodies against bovine hsp70 in total protein extracts of both intact cells and cells subjected to salinity stress. the same polypeptide antigen ...200616893060
surgical site infections in day surgery settings.because major complications are rare in day surgery, minor morbidities such as surgical site infections, which become evident only after patient discharge, have a major impact on the perceived quality of care. we performed an audit of such infections over a oneyear period.200616895493
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