Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| synthesis, antimicrobial, and antitumor activity of a series of palladium(ii) mixed ligand complexes. | mixed ligand complexes of cisdichloromethioninepalladium(ii) with 2-mercaptopyrimidine and 2-aminopyrimidine were synthesized and characterized by elemental analysis, conductivity data, infrared, and 1h nmr and 13c nmr spectra. in these mixed ligand complexes methionine coordinates to palladium through amino nitrogen and sulphur, thus leaving a free carboxylic acid group. the pyrimidine ligand coordinates to metal ion through n3. mixed ligand complexes of cisdichloroethioninepalladium(ii) with c ... | 1991 | 1791468 |
| composition of the outer membrane of proteus mirabilis in relation to serum sensitivity in progressive stages of cell form defectiveness. | a serum-resistant strain of proteus mirabilis was used to determine whether changes in the composition of surface components could be detected following induction of progressive stages of cell form defectiveness by beta-lactam antibiotics. the critical stage was the conversion from filaments to the spheroplast form, which was accompanied by increased susceptibility to the bactericidal action of human serum. inner and outer membranes of the bacterium, its filament form and its spheroplast form we ... | 1991 | 1791430 |
| a possible role of crows in the spread of diarrhoeal diseases in aden. | the indian house crow (corvus splendens) has increased dramatically in number in aden. these birds pollute the environment by dropping their faecal material all over the city. they may accordingly be related to important public health problems. the present work aimed at investigating the possibility that they play a role in the spread of diarrheal diseases. one hundred and fifty crows were collected and their liver, intestine and cloaca examined bacteriologically for enterobacteriaceae and vibri ... | 1991 | 1791416 |
| [spheroplast formation in clinical isolates of gram-negative bacteria by beta-lactam antibiotic in the presence of ca2+ or mg2+]. | induction to cell wall-deficient bacteria has been suggested to be a cause of intractable and opportunistic infection after chemotherapy. spheroplast formation by beta-lactam antibiotic in not so high osmotic pressure was investigated in six species of gram-negative bacteria. some species of gram-negative bacteria were induced to form spheroplast at a high rate by 1:10 concentration of ceftizoxime in the presence of ca2+ or mg2+. especially in 38% of proteus mirabilis and p. vulgaris, more than ... | 1991 | 1791327 |
| [experimental study of polyvalent proteus antigen immunogenicity]. | the experiments on 52 rabbits have been carried out to show the possibility to use proteus antigen with high content of h-component (h1; h2; h3 proteus antigens are most frequently found) for active immunization to make highly specific humoral immunity. the optimal scheme of immunization consists of 3 subcutaneous injections in doses of 0.25-0.25-0.5 mg of antigen within 7 days intervals and 0.25 mg dose of single revaccination. the duration of cycle immunization is 14 days. | 1991 | 1790822 |
| [common aspects of connective hyperplasia of proteus syndrome and collagenoma]. | 1991 | 1789631 | |
| [septic pulmonary embolism, bacterial endocarditis and endocavitary pacemaker]. | the number of patients carrying an endocavitary pacemaker is increasing every day. the presence of a foreign body in right cavities predisposes to the formation of thrombi that can be over infected from distal septic focci usually silent. when a pulmonary embolism is diagnosed in these patients, the initial study must include an echocardiographic exam in order to rule out the presence of an intracardiac thrombosis as the origin of the emboli. we present the case of a patient with and endocardiac ... | 1991 | 1788458 |
| bacterial colonization behaviour: a new virulence strategy in urinary infections? | the urinary bladder resists bacterial colonization and infection by a number of mechanisms, one of which involves the sloughing of colonized uroepithelial cells. pathogens which thus become detached from bladder tissue are rapidly eliminated upon voiding of urine. during a recent study of bacterial colonization by the urinary pathogen, proteus mirabilis, we noted that it colonized glass surfaces such that organisms became widely and evenly dispersed over the surface. in contrast, pseudomonas flu ... | 1991 | 1787823 |
| the bacteriology of cholelithiasis--china versus japan. | cholelithiasis differs considerably from area to area in the world. calcium bilirubinate stones or brown pigment stones are said to predominate in the orient, however, this situation may differ within the orient. in order to compare cases in china and japan, 102 consecutive cases of cholelithiasis operated on in shenyang, china were analyzed for the composition of gallstones and bacterial species isolated from bile in relation to the location and composition of gallstones. in shenyang, calcium b ... | 1991 | 1787607 |
| xylose-lysine-tergitol 4: an improved selective agar medium for the isolation of salmonella. | a study was conducted to evaluate a new selective plating medium for isolating salmonella using pure bacterial cultures, and poultry environmental specimens containing high numbers of competing enteric bacteria. xylose-lysine-tergitol 4 (xlt4) agar was found to strongly inhibit proteus, pseudomonas, providencia, and many other nonsalmonellae and to provide good differentiation between salmonella and citrobacter. the xlt4 medium significantly improved salmonella isolation from chicken farm enviro ... | 1991 | 1784564 |
| proteus syndrome in 7 patients: clinical and genetic considerations. | the proteus syndrome is a congenital hamartomatous disorder delineated in 1983. because of its polymorphic appearance, the syndrome was named after the greek god proteus whose name means much less than the polymorphous much greater than. major clinical findings include hemi hypertrophy, macrodactyly, exostoses, scoliosis, epidermal nevi, haemangiomas, deeply rugated soles of the feet and a variety of deep and subcutaneous masses. we report on 7 new cases of proteus syndrome. all reported cases h ... | 1991 | 1781960 |
| [endolymphatic therapy of children with chronic osteomyelitis of the long tubular bones]. | a study was made of the clinical efficacy and immune status in 40 children with chronic osteomyelitis of long tubular bones. of these, 13 patients received antibiotics endolymphatically and 27 patients were given conventional antibiotic therapy (intramuscularly, intravenously). in view of the predominance of the staphylococcal flora, endolymphatic therapy included the use of gentamycin and lincomycin, the treatment course lasted up to 10 days. endolymphatic antibacterial therapy has been demonst ... | 1991 | 1781222 |
| novel plasmid vectors for gene cloning in pseudomonas. | novel host-vector systems have been developed for gene cloning in the metabolically versatile bacterial genus pseudomonas. we found that a new pseudomonas strain, pseudomonas flavida if-4, isolated from soil, carried two small cryptic plasmids, named pni10 and pni20. they were multi-copy, but not self-transmissible, and the genome size was 3.7 kb for pni10 and 2.9 kb for pni20. several types of cloning vectors containing a kanamycin or streptomycin resistance (kmr or smr) gene were constructed f ... | 1991 | 1778984 |
| induction of l-asparaginase synthesis in vibrio proteus. | studies on l-asparaginase synthesis in v. proteus showed increased synthesis in cultures grown under conditions of moderate aeration (p less than 0.005) after oxygen had been used up from the medium. addition of sodium lactate to the medium at a concentration of 80 mu mole/ml, stimulated l-asparaginase synthesis (2.2 times over control) in moderately-aerated cultures (p less than 0.001). the substrate l-asparagine induced enzyme synthesis when growth conditions were made anaerobic or lactate was ... | 1991 | 1778615 |
| a new plate medium for rapid presumptive identification and differentiation of enterobacteriaceae. | a new selective differential agar medium for rapid presumptive identification of enterobacteriaceae from water and food samples is described (emx id agar). by a combination of fluorogenic and chromogenic substrates, the medium detects the presence of beta-d-glucuronidase, beta-d-galactosidase, beta-d-xylosidase, tryptophane deaminase and h2s; additionally, cytochrome-oxidase and indole production can be demonstrated. this medium provides an inexpensive means for simple and rapid presumptive iden ... | 1991 | 1777382 |
| efficacy and safety of oral cefixime therapy in common infectious diseases in children. | twenty-nine children, including 20 boys and 9 girls, were enrolled in an open trial of oral cefixime therapy for common pediatric infectious diseases. patients aged between 0.5 month and 12 years with a mean +/- standard deviation of 2.3 +/- 2.8 years. the diagnoses included 10 of acute otitis media, 2 of purulent rhinitis, 4 of acute enterocolitis, and 13 of urinary tract infection. most children received 6-12 mg/kg/day of cefixime granules, orally twice daily for more than five days. all 10 ca ... | 1991 | 1776438 |
| rapid isolation and presumptive diagnosis of uropathogens by using membrane filtration and differential media. | random urine samples from hospitalized patients (n = 550) and seeded sterile filtered urine samples (n = 730) were used to test a membrane filtration technique, qualture (future medical technologies international, inc., west palm beach, fla.), for the detection and identification of uropathogens. results for each sample were compared with those obtained by the calibrated loop (0.01 ml) method to demonstrate the sensitivity of the method as a screening tool and the specificity of the presumptive ... | 1991 | 1774240 |
| [common pathogens in burn infection and changes in their drug sensitivity]. | this paper reports common pathogens in burn infection and changes in their drug sensitivity from 1985 to 1988. gram negative rods constituted 55.7% of all pathogens, while 40.5% were gram positive cocci. the former group consisted of 173 strains of pseudomonas aeruginosa (28.5%), 55 proteus (9.1%), 33 serratia (5.4%), 23 klebsiella (3.8%) 19, acinetobacter (3.1%), 18 enterobacter (3.0%) and 17 e. coli (2.8%). among gram-positive cocci, staphylococcus aureus accounted for 32.8%, staphylococcus ep ... | 1991 | 1773380 |
| [the effect of selected antibiotics on microorganisms contaminating boar ejaculate]. | the occurrence of microorganisms, including their total counts in boar native ejaculates, was investigated in two stages; the objective of this investigation also was to determine contamination after the sperms were treated with diluents containing the antibiotics ampicillin, gentamycin, apramycin, cefoxitin, or antibiotic combinations penicillin + streptomycin, ampicillin + cefoxitin, gentamycin + cefoxitin and ampicillin + gentamycin. the representation of bacterial species and total counts of ... | 1991 | 1771724 |
| transmission of proteus syndrome from father to son? | we present a male infant with cranial hemi-hypertrophy, a lymphangioma, a lipoma, and epidermal naevi. a diagnosis of proteus syndrome was made. his father had had a large lymphangioma resected from the right side of the face as a child. we propose that proteus syndrome has been transmitted from father to son. | 1991 | 1770536 |
| [neonatal proteus mirabilis adrenal gland abscess]. | the authors describe a case of unilateral adrenal abscess in a neonate, which was diagnosed at 13 days of age by abdominal ultrasonography. the newborn presented with persistent high white blood cells after proteus mirabilis omphalitis. magnetic resonance imaging and needle aspiration confirmed the diagnosis. this is the second case of proteus mirabilis neonatal adrenal abscess reported in the literature. percutaneous drainage allowed complete recovery. | 1991 | 1768196 |
| resistant strains isolated from bacteremia patients in northern norway. | bacterial isolates from blood cultures in 1985 and 1989 (227 and 258 isolates, respectively), were compared as regards resistance to a series of antimicrobial agents including the more recent beta-lactams and quinolones. an increase in the number of coagulase-negative staphylococcal strains and a decrease in staphylococcus aureus strains were detected, otherwise there were no significant differences in the bacterial patterns in 1985 compared to 1989. except for chloramphenicol, there was no majo ... | 1991 | 1767256 |
| closely linked genetic loci required for swarm cell differentiation and multicellular migration by proteus mirabilis. | the pathogenic bacterium proteus mirabilis exhibits a form of multicellular behaviour called swarming migration. this involves the differentiation of vegetative cells at the colony margin into swarm cells which are long, aseptate, multinucleate, hyper-flagellated filaments able to undergo repeated cycles of co-ordinated population migration and consolidation (reversion to vegetative cells). transposon mutagenesis of uropathogenic p. mirabilis strain u6450 with tn5 generated 4860 chromosomal inse ... | 1991 | 1766373 |
| [cerebral abscess in a general hospital. analysis of 66 consecutive cases]. | analysis of the epidemiologic characteristics of cerebral abscesses and comparison with 2 therapeutic schedules. | 1991 | 1762456 |
| imipenem resistance in proteus mirabilis. | 1991 | 1761458 | |
| typing of proteus mirabilis from clinical sources by computerized analysis of electrophoretic protein patterns. | seventy-five strains of proteus mirabilis (mainly of human origin) were characterized by one-dimensional sds-page of cellular proteins. the strains came from various countries; 24 were from urine, 17 from faeces (including one from a dog and one from a monkey), 12 from the respiratory tract (including nine from sputum), four from a cerebral abscess, 12 from miscellaneous sources and six from unknown sources. the protein patterns, which contained 45 to 50 discrete bands, were highly reproducible. ... | 1991 | 1761440 |
| microbiological exploitation of cardiac glycosides and alkaloids from garcinia kola, borreria ocymoides, kola nitida and citrus aurantifolia. | the four medicinal plants, garcinia kola (roots), borreria ocymoides (leaves), kola nitida (bark) and citrus aurantifolia (roots) were screened for phytochemical components. they were found to contain tannins, phlobatannins, polyphenols, hydroxymethyl anthraquinones, glucides, saponins, alkaloids, cardiac glycosides, flavanoids and reducing compounds. the aqueous and alcoholic extracts as well as alkaloids and cardiac glycosides of the medicinal plants were tested on various pathogenic bacteria. ... | 1991 | 1761433 |
| [control of genetic consequences of vaccinations: electron microscopic analysis of murine synaptonemal complexes]. | the ability of two inactivated bacterial vaccines, from proteus vulgaris and klebsiella pneumoniae, to injure synaptonemal complexes (scs) was studied by means of electron microscopy. the preparations were given intraperitoneally to c57bl/6j male mice during five successive days and testes were fixed 24 h after the last injection. cyclophosphamide was used for positive control. the vaccine from klebsiella given at subtoxic dose, which was about 200 times higher that that given to people during v ... | 1991 | 1761213 |
| [an experimental study of a lactoglobulin preparation against opportunistic bacteria and salmonellae]. | a preclinical study of seven batches of lactoglobulin, a new biological preparation against opportunistic bacteria and salmonellae, has been carried out. high antibacterial activity of the preparation with respect to the virulent forms of salmonella typhimurium, salmonella enteritidis, pseudomonas aeruginosa, klebsiella pneumoniae, proteus vulgaris, proteus mirabilis has been established. the preparation has been shown to be safe and nontoxic. the 4-year term of its storage at a temperature of 6 ... | 1991 | 1759523 |
| occult respiratory failure as a cause of neuropsychiatric symptoms in chest wall deformity and neuromuscular disease. | 1991 | 1759011 | |
| vaccina treatment in some hospital infections. | phenol-treated vaccine was prepared from the usual hospital strains and patients with colorectal cancer were immunized intramuscularly 7-10 days prior to the operation. the efficiency of the vaccine treatment was judged by the incidence of wound infections. the best results were obtained in patients whose previously estimated immunoreactivity was strong or normal. immunospecificity could not be detected in the above treatment. the frequent and inappropriate use of antibiotics in hospitals increa ... | 1991 | 1755327 |
| antibacterial properties of human amniotic membranes. | amniotic membranes are widely used in a multitude of surgical applications and have been shown to reduce bacterial counts and promote healing in infected wounds. antibacterial properties of amniotic fluid are well documented and the presence of many potentially antibacterial factors has been demonstrated. no such factors have yet been found in amniotic membranes. we have applied a direct disc-diffusion susceptibility test to try to establish the possible existence of such a factor. amniotic memb ... | 1991 | 1754577 |
| [early diagnosis and prognosis of bacterial tubulo-interstitial nephritis in children]. | the authors provide the data of a prospective follow-up of children with diseases of the urinary system organs. analysis was made of the role of endogenous and exogenous factors in the formation of abacterial and bacterial tubulointerstitial nephritis (tin) in children with predisposing conditions. it has been established that progression of the tubulointerstitial process can be seen in patients infected with causative agents capable of intracellular parasitizing and persistence in the body. dep ... | 1991 | 1754327 |
| bacteriology of diabetic foot ulcers. | seventy five diabetic foot ulcers were cultured using optimal aerobic and anaerobic microbiologic techniques. there were a total of 223 isolates (162 aerobes and 61 anaerobes) representing an average of 2.97 bacterial species per specimen. mixed organisms were the most common isolates. staphylococcus aureus was the commonest isolate being recovered in 60% of cases. anaerobic organisms were isolated in 46 patients (61.33%) and the most frequent anaerobe isolated was bacteroides fragilis. the othe ... | 1991 | 1752646 |
| environmental contamination during tracheal suction. a comparison of disposable conventional catheters with a multiple-use closed system device. | the extent of airborne environmental bacterial contamination which occurs following tracheal suction has been investigated in patients undergoing intermittent positive pressure ventilation in the intensive therapy unit. two methods of performing suction, one using a conventional open technique and one using a closed system (stericath), have been compared. significantly lower levels of environmental contamination were observed when the closed system was used. | 1991 | 1750600 |
| purification, crystallization and preliminary x-ray diffraction studies of the pvuii endonuclease. | the pvuii endonuclease (pvuiir) is a restriction enzyme from a type ii restriction-modification system of proteus vulgaris coded on plasmid ppvu1. the protein recognizes the dna sequence 5' cag'ctg 3' and shows no sequence homology to other restriction enzymes. this makes pvuiir an interesting subject for structural determination. a purification procedure was developed that yields milligram quantities of the pvuiir from plasmids expressed in the escherichia coli strain hb101. the protein was cry ... | 1991 | 1748988 |
| in vitro activity of the novel cephalosporin gr69153. | the in vitro activity of gr69153 was compared to that of ceftazidime, ceftriaxone, imipenem and gentamicin against a total of 702 recent clinical isolates. mics were determined by a standard agar dilution procedure and two inocula (10(4) and 10(8) cfu) were used throughout. gr69153 inhibited 90% of isolates of escherichia coli, klebsiella pneumonia and proteus mirabilis at less than or equal to 0.25 mg/l and 90% of isolates of pseudomonas aeruginosa at less than or equal to 1 mg/l. citrobacter f ... | 1991 | 1748126 |
| in vitro activity of ro 09-1428 compared to other cephalosporins. | the in vitro activity of ro 09-1428, a new catechol-type parenteral cephalosporin, was compared to that of ceftazidime, e-1040, cefpirome and cefepime against gram-positive and gram-negative organisms. ro 09-1428 inhibited group a streptococci at less than or equal to 0.12 micrograms/ml, and group b, c and g streptococci and streptococcus pneumoniae at 0.5 micrograms/ml, whereas for staphylococcus aureus ro 09-1428 had mics of 8-16 micrograms/ml similar to ceftazidime and e-1040. against pseudom ... | 1991 | 1748124 |
| urinary stone formation in children with prenatally diagnosed uropathies. | among a series of 312 patients with prenatally diagnosed uropathies, 4 male children developed urinary calculi at ages varying from 2 to 5 years. all had upper urinary tract dilatation not associated with vesicoureteric reflux or with renographically defined obstruction; 3 followed proteus urinary infections. strategies for prevention are discussed. | 1991 | 1747733 |
| the current and future impact of antimicrobial resistance among nosocomial bacterial pathogens. | the selection of drug-resistant microorganisms has generally been associated with the widespread use of antimicrobial agents. the emergence of these antimicrobial resistance has an undesirable impact that often severely limits the use of these drugs that could have otherwise been identified as "drugs of first choice." among the most serious drug-resistance problems, the chromosomally mediated type -i beta-lactamase mechanism has become more common among the infecting gram-negative bacteria. type ... | 1992 | 1737440 |
| role of spectrin in amoeba proteus, as studied by microinjection of anti-spectrin monoclonal antibodies. | spectrin is a major protein accounting for about 5% of whole-cell proteins in amoeba proteus, and the precipitation of spectrin by intracellular injection of purified anti-spectrin monoclonal antibodies has a profound effect on cell morphology, motility, and movement-related cell activities in amoebae. thus, amoebae injected with anti-spectrin antibodies show drastic changes in their shape and movement, suggesting that amoeba spectrin plays an important structural role, unlike nonerythroid spect ... | 1992 | 1735457 |
| xanthogranulomatous pyelonephritis: experience in 36 cases. | a retrospective study of 36 patients with xanthogranulomatous pyelonephritis who underwent nephrectomy at our hospital was performed. the disease occurred most frequently in middle-aged women with a history of recurrent urinary tract disorder. there were 2 cases of focal xanthogranulomatous pyelonephritis, 2 associated with emphysematous pyelonephritis, 2 that manifested as fistula formation between the colon or skin, and 1 with deep sinus formation into the hip joint that presented as septic ar ... | 1992 | 1732587 |
| proteus syndrome: musculoskeletal manifestations and management: a report of two cases. | the literature on clinical and roentgenographic findings of proteus syndrome is reviewed. two patients with proteus syndrome who exhibited skeletal deformities and characteristic features such as limb length discrepancy, skin lesions, and soft tissue tumors are reported. persistent difficulties in treating malformed spines and unequal legs, as well as unpredictable tissue reaction to trauma, are discussed. | 1992 | 1732287 |
| direct membrane effects of morphine and endorphins on amoeba proteus. | morphine, leu-enkephalinamide, met-enkephalin, alpha-neoendorphin and its arg8 1-8 fragment increase contractile vacuole output in the freshwater amoeba proteus at 18 microm. significant effects of leu-enkephalin and naloxone are obtained at 180 microm. all compounds have reached their maximal activity at 720 microm. alpha-neoendorphin and leu-enkephalin are inactive in the presence of isotonic, non-penetration sucrose, hence these compounds increase plasma membrane permeability to water. result ... | 1992 | 1731168 |
| breast-feeding and urinary tract infection. | a case-control study was conducted to study the association between breast-feeding and urinary tract infection. case patients were 128 infants aged birth to 6 months with urinary tract infection. control infants were 128 infants admitted to the same ward with an acute illness. the results support the hypothesis that breast-feeding protects infants against urinary tract infection. | 1992 | 1731031 |
| distribution of an l-isoaspartyl protein methyltransferase in eubacteria. | a protein carboxyl methyltransferase (ec 2.1.1.77) that recognizes age-damaged proteins for potential repair or degradation reactions has been found in all vertebrate tissues and cells examined to date. this enzyme catalyzes the transfer of methyl groups from s-adenosylmethionine to the carboxyl groups of d-aspartyl or l-isoaspartyl residues that are formed spontaneously from normal l-aspartyl and l-asparaginyl residues. a similar methyltransferase has been found in two bacterial species, escher ... | 1992 | 1729230 |
| staphylococcal l-asparaginase: enzyme kinetics. | the ph optimum of purified staphylococcal l-asparaginase (ec 3.5.1.1) was found to be between 8.6 and 8.8. the temperature optimum was 30 degrees-32 degrees c and the highest reaction rate occurred at 30 degrees c. the km of the enzyme calculated from lineweaver-burk plot was 3.71 x 10(-2) m. besides l-asparaginase, the substrate specificity of enzyme was restricted to n-alpha-acetyl-l-asparagine. d-asparagine, l-aspartic acid and d-glutamic acid were competitive inhibitors. hg2+ and cu2+ cation ... | 1991 | 1726615 |
| review of clinical experience in the united states with cefpodoxime proxetil in adults with uncomplicated urinary tract infections. | two controlled united states trials compared the safety and efficacy of cefpodoxime proxetil (100mg twice daily) with either cefaclor (250mg 3 times daily) or amoxicillin (250mg 3 times daily) in patients with uncomplicated urinary tract infections. treatment duration was 7 days. 307 of 762 patients treated with cefpodoxime proxetil, 99 of 190 treated with cefaclor, and 57 of 185 treated with amoxicillin were evaluable for efficacy. 311, 99 and 59 pathogens were isolated from cefpodoxime proxeti ... | 1991 | 1726207 |
| the structure of proteus penneri strain 14-o-specific polysaccharide containing d- and l-alanine. | 1991 | 1725142 | |
| [serotyping of proteus using a rapid system]. | a rapid method for proteus serologic typing is suggested, based on soviet commercial diagnostic adsorbed type sera. the rapid system involves replacing two-staged typing with one-staged, making use of polyvalent sera constructed by uniting 8-9 type sera according to the suggested schemes. o- and h-antigens are types in accordance with special tables. fifteen o-antigens and 4 h-antigens, whose total incidence among proteus clinical strains is under 2 percent, cannot be typed with the use of this ... | 1991 | 1724043 |
| immunoblot studies to analyze antibody to the rickettsia typhi group antigen in sera from patients with acute febrile cerebrovasculitis. | in 1986, an unusual syndrome of acute febrile cerebrovasculitis in the piedmont region of virginia was reported. all patients had encephalopathy and prior exposure to both a sylvan environment and flea-infested animals. the initial serological studies suggested a rickettsial origin, corroborating clinical, epidemiological, and histopathological findings. sera from four of five patients were subsequently studied by immunoblotting. unabsorbed and absorbed sera were tested with electrophoresed and ... | 1991 | 1723073 |
| evolution of the ferric enterobactin receptor in gram-negative bacteria. | using sodium dodecyl sulfate-polyacrylamide gel electrophoresis of iron-deficient and replete cell envelopes, 59fe-siderophore uptake studies, and western immunoblots and cytofluorimetric analyses with monoclonal antibodies (mabs), we surveyed a panel of gram-negative bacteria to identify outer membrane proteins that are structurally related to the escherichia coli k-12 ferric enterobactin receptor, fepa. antibodies within the panel identified fepa epitopes that are conserved among the majority ... | 1991 | 1717434 |
| differentiation of enterobacteriaceae, pseudomonas aeruginosa, and bacteroides and haemophilus species in gram-stained direct smears. | the accuracy of examination of the gram-stained direct smear to classify presumptively gram-negative rods into three morphotype groups, that is, (a) enteric bacteria, (b) pseudomonas, and (c) bacteroides or haemophilus, was evaluated. randomly selected clinical strains (4-9) each of escherichia coli, klebsiella pneumoniae, proteus mirabilis, ps. aeruginosa, b. fragilis, and h. influenzae were used to produce peritonitis or subcutaneous abscesses in mice. a gram-stained direct smear was prepared ... | 1991 | 1716190 |
| [stable erythrocyte reagents for detecting antigen-binding lymphocytes]. | fixation of native chicken red cells with a solution containing 14.4 percent of acetate aldehyde and 0.85 percent of nacl (ph 7.0-7.2) permitted preparation on the basis of these red cells of stable immunoreagents for the detection of antigen-fixing lymphocytes using any (optimal for the antigen used) procedure. experiments have demonstrated a high efficacy of detecting staphylococcal, proteus, or ps. aeruginosa etiology of pyoinflammatory and septic conditions in indirect rosette formation test ... | 1991 | 1715944 |
| kinetics of haemolytic activity in proteus strains. | proteus species produces toxins and constitutes a causative agent of some chronic and recurrent infections. for the study of haemolytic activity and the production and inhibition kinetics, a total of 140 local isolates were diagnosed and examined by the general biochemical methods, and their ability of haemolysis were tested by both direct and indirect methods utilizing the enrichment procedure for all strains. two antibiotics, erythromycin and keflex (cephalexin), were tested for the study of h ... | 1990 | 1715649 |
| [the etiologic structure of acute intestinal infections caused by opportunistic microorganisms (irkutsk, 1984-1988)]. | the authors analyze the results of bacteriologic studies carried out in patients with intestinal infections at the laboratory of the sanitary epidemiologic center of the town of irkutsk over 1984-1988. the records of 24,490 case histories show infections induced by opportunistic microflora in 37 percent of cases. the most frequent etiologic agents were staphylococcus aureus and enterobacteriaceae, genera: proteus, enterobacter, citrobacter, klebsiella, hafnia. analysis of the incidence of acute ... | 1991 | 1715437 |
| structural and immunochemical studies of o-specific polysaccharide of proteus vulgaris 5/43 belonging to ox19 group (o-variants). | o-specific polysaccharide was obtained on mild acid degradation of lipopolysaccharide of proteus vulgaris 5/43 belonging to ox19 (o-variants). it was found to contain d-glucose, 2-acetamido-2-deoxy-d-glucose, and 2-acetamido-2,6-dideoxy-l-glucose (quinac, n-acetyl-l-quinovosamine) in the ratio of about 1:2:1, the last-named sugar being rather uncommon for bacterial antigens. a computer-assisted 13c-nmr-based analysis, methylation analysis, and selective cleavage with anhydrous hydrogen fluoride ... | 1991 | 1715274 |
| interspecies compatibility of selenoprotein biosynthesis in enterobacteriaceae. | several species of enterobacteriaceae were investigated for their ability to synthesize selenium-containing macromolecules. seleniated trna species as well as seleniated polypeptides were formed by all organisms tested. two selenopolypeptides could be identified in most of the organisms which correspond to the 80 kda and 110 kda subunits of the anaerobically induced formate dehydrogenase isoenzymes of e. coli. in those organisms possessing both isoenzymes, their synthesis was induced in a mutual ... | 1991 | 1710885 |
| [the isolation of a pure culture of proteus hauseri from associated bacteria]. | a simple method for isolation of p. vulgaris and p. mirabilis pure culture from associations with other microorganisms has been developed. medium for inoculation of a bacterial culture suspected of an association contains glycerin and asparaginic acid. the suggested method is simple and highly effective, reproducible at any bacteriologic laboratory, and is particularly valuable for improving the quality of etiologic diagnosis of pyoseptic diseases. | 1990 | 1699046 |
| [primary structure of the 5'-region of yersinia pestis 16s rrna]. | the sequence of 5'-region (16-296 n.) of 16s rrna of plague agent (yersinia pestis) was determined after sequencing of cloned cdna fragments complementary to this region. when compared to the same region of 16s rrna of escherichia coli and proteus vulgaris this region revealed 91.8% and 87.2% of homology, respectively. the sequences specific for y. pestis 16s rrna were localized and their secondary structure position was discussed. | 1990 | 1698255 |
| cloning, partial sequence, expression, and antigenic analysis of the filamentous hemagglutinin gene of bordetella pertussis. | the gene coding for the filamentous hemagglutinin (fha), one of the main factors involved in mediating adherence of bordetella pertussis to ciliated host cells, was cloned in escherichia coli, and the 3,500-base-pair nucleotide sequence encoding the amino-terminal region was determined. molecular cloning, together with the characterization of recombinant fha-related proteins produced in e. coli, revealed that the primary translation product is a protein of about 370 kilodaltons (kda). the mature ... | 1990 | 1696934 |
| sandwich enzyme immunoassays for detection of salmonella typhi. | enzyme immunoassays were developed using monoclonal antibodies raised against somatic (o), flagellar (h) and capsular (vi) antigens of salmonella typhi. the assay based on anti-o monoclonal antibodies could specifically detect s. typhi and soluble lipopolysaccharide (lps) isolated from s. typhi. anti-h moabs detected motile s. typhi and soluble flagellar antigen. monoclonal antibodies against capsular polysaccharide could detect vi-containing s. typhi as well as soluble vi antigen. the three ass ... | 1990 | 1693382 |
| immunological characterization of lipopolysaccharides from proteus strains used in weil-felix test and reactivity with patient sera of tsutsugamushi diseases. | immunological analyses of lipopolysaccharides (lps) isolated from proteus strains ox2, ox19, and oxk used as antigens of weil-felix (wf) test, were performed by quantitative agglutination, enzyme-linked immunosorbent assay (elisa), and immunoblotting. antisera against lps and whole cells (wc) of the three proteus strains reacted with homologous lps but not with heterologous lps, and the reaction was inhibited by the o-polysaccharide fraction isolated from the homologous lps except ox19-lps, whic ... | 1990 | 1693170 |
| the structure of o-specific polysaccharide of proteus vulgaris 019 lipopolysaccharide. | 1990 | 1691576 | |
| hlyb-dependent secretion of hemolysin by uropathogenic escherichia coli requires conserved sequences flanking the chromosomal hly determinant. | the synthesis and secretion of hemolysin (hlya) by escherichia coli are governed by four contiguous genes (hlycabd) that are closely conserved on plasmids and, among human pathogenic strains, on the chromosome. we have previously shown that in plasmid phly152 the coexpressed synthesis and export functions are uncoupled by intraoperon transcription termination, which is in turn alleviated by antitermination dictated in cis by a region upstream of the hly operon. in this study we describe an analo ... | 1990 | 1689714 |
| receptors for human plasminogen on gram-negative bacteria. | a total of 188 strains representing 11 species of gram-negative bacteria were examined for the ability to interact with human plasminogen. highly purified human plasminogen was labeled with 125i, and its uptake by different bacterial strains was measured. all 14 strains of haemophilus influenzae and all 13 strains of branhamella catarrhalis tested were positive with respect to plasminogen uptake. also, eight species belonging to the family enterobacteriaceae were tested, and of those, proteus mi ... | 1990 | 1688419 |
| use of an amoeba proteus model for in vitro cytotoxicity testing in phytochemical research. application to euphorbia hirta extracts. | amoeba proteus is proposed as a low-cost multi-purpose biochemical tool for screening and standardizing cytotoxic plant extracts with possible application in the laboratories of developing countries. advantages and limitations of this test are examined and different mathematical treatments (probit analysis versus curve fitting to von bertalanffy and hill functions) are investigated. known anti-cancer (doxorubicin, daunorubicin, dacarbazine, 5-fluorouracil) and antiparasitic (emetine, dehydroemet ... | 1991 | 1686625 |
| fimbrial types among respiratory isolates belonging to the family enterobacteriaceae. | bacterial attachment is believed to be an early step in gram-negative nosocomial pneumonia. the frequency of fimbria-associated adhesins among respiratory pathogens has not been studied in detail. in this study isolates belonging to the family enterobacteriaceae, prospectively obtained from intensive care unit patients who were suspected of having nosocomial pneumonia, were examined for fimbria-associated adhesins. type 3, p, type 1, and other fimbrial phenotypes were identified by specific hema ... | 1991 | 1685495 |
| antimicrobial activities of piperacillin alone and in combination with tazobactam against beta-lactamase-producing bacteria. | tazobactam (ytr 830), a new beta-lactamase inhibitor, was evaluated for its effect in combination with piperacillin, a broad spectrum, but beta-lactamase sensitive, penicillin, against 14 common bacteria. a total of 1,086 clinical isolates from different clinical specimens were tested for beta-lactamase production by the rapid chromogenic cephalosporin method. their susceptibilities to piperacillin alone and in combination with tazobactam in a ratio of 8:1 by the agar dilution method were evalua ... | 1991 | 1685175 |
| ability of uropathogens to bind to tamm horsfall protein-coated renal tubular cells. | epithelial cells were isolated from a healthy human kidney and found to be of normal, renal tubular origin. the cells were maintained in tissue culture and found to secrete tamm horsfall protein (thp). three strains of uropathogenic escherichia coli, and one each of proteus mirabilis and pseudomonas aeruginosa were found to adhere to the thp-coated tubular cells. extraneous thp bound to all the organisms except p fimbriated e. coli, and caused a reduction in the adhesion of type 1 fimbriated e. ... | 1991 | 1683041 |
| long-acting chloramphenicol versus intravenous ampicillin for treatment of bacterial meningitis. | in most developing countries, bacterial meningitis (bm) is associated with a high case-fatality rate. the search for a simple, convenient, and inexpensive antibiotic treatment remains a priority. in this study, a non-blinded, multicentre, randomised clinical trial of 528 cases of bm was done in two hospitals in mali and niger, between march, 1989, and may, 1990, to see whether a double injection of long-acting chloramphenicol (on admission to hospital and 48 h later) is as effective as a course ... | 1991 | 1681224 |
| antigenic and immunogenic activity of flagella and fimbriae preparations from uropathogenic proteus mirabilis. | the antigenic and immunogenic activities of fimbriae and flagella from three uropathogenic strains of proteus mirabilis were compared. flagella were obtained by mechanical treatment and fimbriae were isolated from cells by heat shock, ammonium sulfate precipitation, sodium deoxycholate and urea treatment, and gel filtration. both preparations inoculated to mice demonstrated high antigenicity. titers up to 1:80,000 were determined by an enzyme-linked immunosorbent assay either against the homolog ... | 1991 | 1680542 |
| proteus mirabilis flagella and mr/p fimbriae: isolation, purification, n-terminal analysis, and serum antibody response following experimental urinary tract infection. | urinary tract infection with proteus mirabilis may lead to serious complications, including cystitis, acute pyelonephritis, fever, bacteremia, and death. in addition to the production of hemolysin and the enzyme urease, fimbriae and flagellum-mediated motility have been postulated as virulence factors for this species. we purified mannose-resistant/proteuslike (mr/p) fimbriae and flagella from strains cft322 and hu2450, respectively. electron microscopy revealed highly concentrated preparations ... | 1991 | 1680106 |
| prevalence of nosocomial infections in a saudi arabian teaching hospital. | the rate of nosocomial infections was determined during a 9-month study in a 6-year-old hospital in north saudi arabia. the overall rate of nosocomial infection in the hospital was 2.2%. the rates in the different services varied. the highest were in the special care baby unit (13.5%) and intensive care unit (6%). in the other services it ranged from 1 to 3.5%. the common causal agents of documented infections were escherichia coli (81), pseudomonas aeruginosa (55), staphylococcus aureus (43), k ... | 1991 | 1675653 |
| cellular immunodeficiency in protein-losing enteropathy. predominant reduction of cd3+ and cd4+ lymphocytes. | cellular immunological abnormalities were studied in a patient with protein-losing enteropathy associated with constrictive pericarditis. analysis of lymphocyte subpopulations in peripheral blood showed lymphopenia with a decrease of cd3+ and cd4+ t cells, whereas cd8+ lymphocytes, b cells and nk cells were within the normal range. fecal loss of lymphocytes as a cause of lymphopenia was evidenced by a marked excretion of 111-indium-labeled peripheral blood mononuclear cells via stool. proliferat ... | 1991 | 1670632 |
| [urinary tract infection in an ambulatory population: epidemiological analysis of the etiology and antibiotic resistance of isolated gram-negative strains]. | 15,892 urinocultures belonging to out-patients admitted to chieti ulss 04, from january '85 to december '89 were studied. among the examined samples, the positive urinocultures were 4255 (26.8%) with a prevalence in the female sex (78.6%). during the year e. coli was the most frequently identified organism (55.8%) without significant changes. 25.7% was the isolation percentage of morganella-proteus-providencia (mpp). in order to plan a right antibiotic-therapy the resistance of isolates to 19 ch ... | 1991 | 1670056 |
| active efflux of ofloxacin by a highly quinolone-resistant strain of proteus vulgaris. | a clinical isolate of proteus vulgaris, strain 881051, highly resistant to ofloxacin (mic for ofloxacin; 800 mg/l) possessed three types of resistance mechanisms to ofloxacin. the supercoiling activity of dna gyrase from this strain was barely inhibited by ofloxacin, the id50 value of ofloxacin against the enzyme was 2700 times higher than that against the enzyme from a susceptible strain, p. vulgaris 08602. in the outer membrane of this resistant strain, no porin protein of 37 kda was detectabl ... | 1991 | 1667782 |
| mouse models of short- and long-term foreign body in the urinary bladder: analogies to the bladder segment of urinary catheters. | catheter-associated bacteriuria is the most common infection occurring in hospitals, where urethral catheters are generally in place for a few days, and in nursing homes, where catheters may be in place for months or years. we developed murine models with intrabladder urinary catheters for studying complications of bacteriuria in short- and long-term catheterization. in the short-term model, a catheter segment was inserted transurethrally and lay free within the bladder lumen. half of the animal ... | 1991 | 1666147 |
| [a case of proteus syndrome]. | 1991 | 1665045 | |
| in vitro activity and stability against novel beta-lactamases of investigational beta-lactams (cefepime, cefpirome, flomoxef, sce2787 and piperacillin plus tazobactam) in comparison with established compounds (cefotaxime, latamoxef and piperacillin). | the therapeutic perspectives of flomoxef, sce 2787, cefpirome, cefepime, latamoxef, cefotaxime and of piperacillin plus tazobactam were comparatively evaluated by their in vitro activity against 1119 clinical isolates of 83 bacterial species. escherichia coli, klebsiella spp. enterobacter sakazakii, proteus spp. and shigella spp. were about equally susceptible to the cephalosporins (mic90: 0.06 to 0.5 mg/l), while the mic90 for piperacillin plus tazobactam was between 2 and 16 mg/l. enterobacter ... | 1991 | 1664418 |
| pyrophosphate inhibition of proteus mirabilis-induced struvite crystallization in vitro. | struvite (mgnh4po4.6h2o) crystals, the major mineral component of infectious urinary calculi, were produced in vitro by growth of a clinical isolate of proteus mirabilis in artificial urine. p. mirabilis growth and urease-induced struvite production were monitored by phase contrast light microscopy and measurements of urease activity, ph, ammonia concentrations, turbidity, and culture viability. in the absence of pyrophosphate, struvite crystals appeared within 3-5 h due to the urease-induced el ... | 1991 | 1663844 |
| [an electron microscopic study of the interaction of bacterial intestinal microflora and rotavirus virions]. | transmission electron microscopy has revealed the capacity of rotaviruses for adsorption on escherichia coli, klebsiella pneumoniae, proteus mirabilis cells and the absence of such capacity with respect to enterococcus faecalis, lactobacillus casei and edwardsiella tarda. different degrees and a varying character of the adsorption of rotaviruses by the representatives of the opportunistic group of bacteria have been established, which may aggravate the course of rotavirus diarrhea due to the agg ... | 1991 | 1661985 |
| beta-lactamase inhibitors: relation between kinetic data and in-vitro synergism studies. | because of the need for control of beta-lactamase-mediated resistance and the recent development of tazobactam, the author has examined the inhibitors of various beta-lactamases for their effectiveness. on the basis of the kinetic data, tazobactam exhibited the highest affinity to various beta-lactamases. a combination with tazobactam was found to enhance the effectiveness of piperacillin against s. aureus producing penicillinase, e. coli producing tem-1 or tem 2 enzymes and class i beta-lactama ... | 1991 | 1661596 |
| pathogenesis of intraabdominal abscess formation: abscess-potentiating agents and inhibition of complement-dependent opsonization of abscess-inducing bacteria. | bacteroides fragilis and escherichia coli are synergistic in the production of intraabdominal abscesses. however, these bacteria initiate abscess formation only when inoculated with an agent such as autoclaved colonic contents (acc) or bran (a fiber analogue). the mechanism of action of the abscess-potentiating agent was studied. opsonins in normal mouse serum were determined for phagocytic killing by murine neutrophils of b. fragilis and e. coli. opsonization required fixation of complement by ... | 1991 | 1659600 |
| effects of dietary calcium, magnesium and phosphorus on the formation of struvite stones in the urinary tract of rats. | after feeding various diets we studied the effects of dietary calcium, magnesium and phosphorus on the formation of struvite stones in rats with urinary tract infections, and also studied the effects of the administration of vitamin d3 and aluminium gel on stone formation. a low-magnesium diet decreased urinary magnesium and prevented stone formation, but a medium-calcium diet did not significantly decrease stone weight. a high-calcium diet decreased urinary phosphorus and inhibited stone format ... | 1991 | 1659017 |
| imipenem- and meropenem-resistant mutants of enterobacter cloacae and proteus rettgeri lack porins. | carbapenems such as imipenem and meropenem are not rapidly hydrolyzed by commonly occurring beta-lactamases. nevertheless, it was possible, by mutagenesis and selection, to isolate mutant strains of enterobacter cloacae and proteus rettgeri that are highly resistant to meropenem and imipenem. two alterations were noted in the e. cloacae mutants. first, the mutant strains appeared to be strongly derepressed in the production of beta-lactamases, which reached a very high level when the strains wer ... | 1991 | 1656855 |
| transposon mutagenesis in proteus mirabilis. | a technique of transposon mutagenesis involving the use of tn5 on a suicide plasmid was developed for proteus mirabilis. analysis of the resulting exconjugants indicated that tn5 transposed in p. mirabilis at a frequency of ca. 4.5 x 10(-6) per recipient cell. the resulting mutants were stable and retained the transposon-encoded antibiotic resistance when incubated for several generations under nonselective conditions. the frequency of auxotrophic mutants in the population, as well as dna-dna hy ... | 1991 | 1655704 |
| the reca-recbcd dependent recombination pathways of serratia marcescens and proteus mirabilis in escherichia coli: functions of hybrid enzymes and hybrid pathways. | the physical maps of cloned recbcd gene regions of serratia marcescens and proteus mirabilis were correlated to genes located in this region. the genes thya, recc, recb, recd and arga were organized as in escherichia coli. the 3 rec genes code for the 3 different subunits of the recbcd enzyme and produced enzymes promoting recombination and repair of uv damage in e coli. the recbcd-dependent stimulation of recombination at specific nucleotide sequences called chi (chi-activation) was determined ... | 1991 | 1655050 |
| in-vitro evaluation of ampicillin/brobactam and comparison with other beta-lactam antibiotics. | the ability of brobactam to inhibit beta-lactamases and the in vitro activity of ampicillin combined with brobactam was compared with another beta-lactamase inhibitor, clavulanic acid, and other beta-lactam antibiotics. both inhibitors showed good and similar activity against staphylococcal penicillinase and most broad-spectrum beta-lactamases found in the enterobacteriaceae, whether plasmid or chromosomally mediated. both inhibitors were less active against chromosomally mediated cephalosporina ... | 1991 | 1646779 |
| comparative in vitro activities of amoxicillin-clavulanate, ampicillin-sulbactam and piperacillin-tazobactam against strains of escherichia coli and proteus mirabilis harbouring known beta-lactamases. | strains of escherichia coli (n = 124) and proteus mirabilis (n = 29) harboring known beta-lactamases were analyzed as to their susceptibility to ampicillin, amoxicillin, and piperacillin alone and in combination with sulbactam, clavulanate, and tazobactam. with tem 1-producing e. coli, a correlation between specific beta-lactamase activity and the mic of piperacillin and ampicillin-sulbactam was observed. these strains also showed significant differences in susceptibilities to the various combin ... | 1991 | 1646771 |
| ofloxacin versus trimethoprim/sulfamethoxazole in the treatment of uncomplicated urinary tract infection. | a multicenter randomized study was conducted to compare the efficacy and safety of ofloxacin with that of trimethoprim/sulfamethoxazole (tmp/smx) in the treatment of uncomplicated urinary tract infection in adults. patients were randomized to receive either oral ofloxacin 200 mg daily for three days (102 patients), or oral tmp/smx 160 mg/800 mg twice daily for seven days (100 patients). the pathogen was eradicated in 73 (97.3%) of the 75 evaluable patients receiving ofloxacin and in 66 (97.1%) o ... | 1992 | 1638586 |
| potential for local and systemic bacterial infection in some occupational groups in benin city, nigeria. | many third world countries may not have adequate facilities for rapid sensitivity testing of antibiotics as an aid to diagnosis and chemotherapy. it may therefore be valuable to relate bacterial carriage on the skin to type of work a person does. | 1992 | 1636931 |
| [emphysematous pyelonephritis. a serious complication of diabetes mellitus]. | pyelonephritis emphysematous (pe) is a life threatening renal infection which is observed practically exclusively as a serious complication of diabetes mellitus. 95% of the 73 cases which have been reviewed were found in diabetic patients. the symptomatology resembles that of severe acute pyelonephritis but the disease differs from this in that, in pe, emphysema develops in the actual renal parenchyma and/or in the perirenal tissues. the most important single factor in the etiology appears to be ... | 1992 | 1631968 |
| reliability of a bioluminescence atp assay for detection of bacteria. | the reliability of bioluminescence assays which employ the luciferin-luciferase atp-dependent reaction to evaluate bacterial counts was studied, both in vitro and on urine specimens. bioluminescence and cultural results for the most common urinary tract pathogens were analyzed. furthermore, the influence of the culture medium, of the assaying method, and of the phase of growth on bioluminescence readings was studied. results show that proteus, providencia, and morganella strains are not correctl ... | 1992 | 1629329 |
| in vitro activation of the serratia marcescens hemolysin through modification and complementation. | the hemolytic activity of serratia marcescens is determined by two polypeptides, termed shla and shlb. shla is synthesized as an inactive precursor (shla*) and secreted with the help of shlb, which is located in the outer membrane. in this study, it is shown that a cell lysate containing shlb as well as partially purified shlb converted shla* to the active shla hemolysin. shla remained active after removal of shlb by column chromatography. in contrast to the stable modification of shla* by shlb, ... | 1992 | 1629165 |
| production and characterization of a monoclonal antibody to the o-acetylated peptidoglycan of proteus mirabilis. | a monoclonal antibody (pmpg5-3) specific for the o-acetylated peptidoglycan of proteus mirabilis 19 was produced by an ns-1 myeloma cell line and purified from ascites fluid by a combination of ammonium sulfate precipitation and affinity chromatography. the monoclonal antibody (an immunoglobulin m) was characterized by a competition enzyme-linked immunosorbent assay to be equally specific for both insoluble and soluble o-acetylated peptidoglycan but weakly recognized chemically de-o-acetylated p ... | 1992 | 1629161 |
| endophthalmitis following extracapsular cataract surgery: a review of 32 cases. | thirty two cases of endophthalmitis following extracapsular cataract surgery that had occurred within our department and had undergone intraocular diagnostic tap between may 1982 and may 1991 were reviewed. an infectious agent was identified in 20 cases (62.5%). the commonest organism was staphylococcus epidermidis (11 cases) (55%). proteus was the only gram negative organism identified (four cases) (20%). both of these organisms were associated with a favourable visual outcome. in the culture p ... | 1992 | 1627512 |
| a case of murine typhus in queensland. | to present a case of murine (endemic) typhus, the first to be reported within the last 30 years in australia. | 1992 | 1625620 |
| klebsiella aerogenes urease gene cluster: sequence of ured and demonstration that four accessory genes (ured, uree, uref, and ureg) are involved in nickel metallocenter biosynthesis. | the region located immediately upstream from the klebsiella aerogenes urease structural genes was sequenced and shown to possess an open reading frame capable of encoding a 29.8-kda peptide. deletions were generated in this gene, denoted ured, and in each of the genes (uree, uref, and ureg) located immediately downstream of the three structural genes. transformation of the mutated plasmids into escherichia coli resulted in high levels of urease expression, but the enzyme was inactive (deletions ... | 1992 | 1624427 |
| calculous pyelonephritis. | the authors report on 48 patients with calculous pyelonephritis. urinary obstruction was caused by renal calculi in 21 patients and ureteral calculi in 27. urine cultures were positive in 87.5% and bacteremia was seen in 70%. the common organisms in urine and blood culture were e. coli, proteus and klebsiella. septic shock occurred in 10 (20.8%) out of 48 patients. calculous pyelonephritis with urinary obstruction is a very serious condition. | 1992 | 1624252 |