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pyridoxal 5'-phosphate-dependent histidine decarboxylase. inactivation by alpha-fluoromethylhistidine and comparative sequences at the inhibitor- and coenzyme-binding sites.pyridoxal phosphate-dependent histidine decarboxylase from morganella morganii am-15 was inactivated by (s)-alpha-fluoromethylhistidine by a pseudo first-order reaction, with ki and k inact values of 0.1 mm and 32.2 min-1, respectively, and was most efficient at ph 6.5-7.0. both l-histidine and the competitive inhibitor, l-histidine methyl ester, protected against inactivation. the apoenzyme was not inactivated. these findings indicate that inhibition is a mechanism-based process. under optimal ...19863733745
[nonspecific pyelonephritis and the formation of urinary calculi in urogenital tuberculosis].the course of urogenital tuberculosis is complicated by unspecific bacterial infections of the urinary tract and nephrolithiasis. among 605 patients with bacteriological or histological verified urogenital tuberculosis, 122 patients (20%) developed unspecific bacterial urinary infection--commonly caused by e. coli, proteus and pseudomonas--and 57 patients (9.4%) showed nephrolithiasis. in 42% of the lithiasis patients an urinary tract infection simultaneously occurred. 22 calculi are analyzed by ...19863739437
acute mastoiditis. influence of antibiotic treatment on the bacterial spectrum.the work was undertaken to investigate the spectrum of bacteria responsible for acute mastoiditis and to find out whether it is affected by intake of antibiotics prior to surgical treatment. the records were reviewed of 22 children with acute mastoiditis in whom mastoidectomy had been performed and bacterial cultures obtained. eleven of the patients had had antibiotic treatment prior to admission (9 penicillin v and 2 erythromycin). streptococcus pneumoniae was found in 8 of the purulent dischar ...19863739692
pleiotropy versus heterogeneity in proteus syndrome. 19863748701
cushing's syndrome--transitory immune deficiency state?a 28 year old female patient with cushing's syndrome due to an adrenal adenoma also suffered from recurrent urinary infections (proteus), tonsillitis (streptococcus), permanent candidiasis and perimandibular abscess (staphylococcus pyogenes). suppression of cellular and humoral immunity was confirmed by in vitro tests. after successful right adrenalectomy the clinical signs of cushing's syndrome disappeared and no evidence of either bacterial or fungal infection were noted one year postoperative ...19863748930
production and degradation of indole by gram-negative bacteria.the indole reaction is performed in various modifications with regard both to the reagents and to the media used. especially the medium has hitherto attracted little attention and there are divergent recommendations for its composition. the comparison of some broths without and with addition of tryptophan after incubation with different indole-positive species revealed that tryptone without tryptophan yielded stronger reactions than with tryptophan added. other broths showed stronger reactions w ...19863754676
prevention of primary wound infection in neurosurgical patients: a 10-year study.two neurosurgeons in private practice at three community hospitals followed a regimen of antimicrobial prophylaxis for 10 years. no primary wound infection occurred in a series of 2000 consecutive major operations. the study gives rise to certain recommendations for the prevention of postoperative sepsis in neurosurgical patients.19863736793
[surgical therapy of intracardiac infected pacemaker electrodes and catheter remnants].with the increase of pacemaker and central venous catheter implantation the number of intracardiac infections now constitutes a significant problem. twenty patients between 22 and 77 years of age admitted for intracardial infected pacemaker probes or central venous catheter remnants presented with recurring attacks of endocarditic fever; in one case multiple pulmonary abscesses resulted from septic embolization. staphylococci were the most frequently involved organism (75%). risk factors leading ...19863705686
prevalence of proteus species in urinary tract infections. 19863759166
cloning of urease gene sequences from providencia stuartii.providencia stuartii was the most prevalent isolate recovered from urine specimens taken weekly over a 1-year period from 51 nursing home patients with urinary catheters in place. thirty percent of the isolates were urease positive. urease, which is implicated in renal stone formation, was shown to be transmissible on an 82-kilobase conjugative plasmid in one isolate. plasmid dna isolated from this strain was digested with ecori, ligated into the ecori site of pbr322, and used to transform esche ...19863759233
role of proteeae in diarrheal disease. 19863760149
[effect of cefminox on bacterial flora in human adult feces].cefminox (cmnx), a new cephamycin, was administered by one shot intravenous injection twice daily with a dose of 1,000 mg each time for 5 days to seven healthy male volunteers whose ages ranged from 21 to 28 years (mean: 25 years) and body weights were from 60 to 92 kg mean: 72 kg). the effect of the drug on fecal bacterial flora was investigated and the concentrations of the drug in feces were measured on 5th day before the treatment, on 0, 3rd, and 5th day (the final day of the treatment) duri ...19863761549
antibiotic resistance and conjugative r plasmids in clinical isolates of enterobacteriaceae in peninsular malaysia.twenty-five strains of enterobacteria, isolated from man in peninsular malaysia and consisting of seven enterobacter spp., five escherichia coli, five salmonella spp., four klebsiella spp., two shigella spp., one proteus sp. and and one providencia sp., were tested for antibiotic resistance and conjugative r plasmids. they were all sensitive to nalidixic acid and resistant to at least three antibiotics. the number of resistances ranged from 3 to 11 antibiotics, including cefoperazone and sisomic ...19863726978
[microfloral changes in the small and large intestines of chronic enteritis patients on diet therapy including sour milk products].the microflora of the small and large intestines was studied in 105 patients with chronic enteritis. significant amounts of various microorganisms were detected in the small intestine. the content of e. coli, bacteroids, bifidobacteria in feces diminished, while that of staphylococcus, enterococcus and fungi rose. the patients were given diet n 4 (intended for enteritis patients) containing lactic acid products (200 ml, 5 times/day, during 24 days); 38 patients received shubat, 30--koumiss and 3 ...19863765530
[a case of necrotic infection in the face]. 19863757109
antibacterial interaction of cefuroxime and serum from uraemic patients.a strain of proteus rettgeri was exposed to combinations of subinhibitory concentrations of cefuroxime and serum from normal subjects and from uraemic patients (on conservative and on haemodialysis treatment) and the antibacterial activity of these was determined. cefuroxime and normal serum gave a synergistic effect. compared with controls, the combination of cefuroxime and uraemic serum from patients on conservative treatment showed a reduced inhibitory effect. the antibacterial activity of th ...19863771426
phosphorylation of amoeba g-actin and its effect on actin polymerization.mass culture of amoeba proteus enabled us to do biochemical studies on this organism. actin and profilin were purified from amoeba to examine actin phosphorylation and polymerization. the apparent molecular weight of amoeba actin was 44,000, and its isoelectric point was 5.8. the apparent molecular weight of amoeba profilin was 12,000, and its isoelectric point was 4.9. it reduced the rate of actin polymerization as reported in the cases of profilins from other organisms. a protein of mr = 44,00 ...19863771554
[osmotic homeostasis in the freshwater ameba].structural and physiological osmotic adaptations of freshwater protozoa are reviewed using amoeba proteus as an example. a particular emphasis is given to the contractile vacuole. recent results on the effects of exogenous atp and vasopressin on the contractile vacuole are also presented.19863772815
a model for experimental bacterial cystitis in the dog.to induce an experimental model of bacterial cystitis, ten preconditioned dogs underwent bladder irritation with a 0.1% alcoholic solution of salicylic acid followed in 24 hours by an intravesicular infusion of proteus mirabilis. the dogs were observed for the following 14 days (five dogs) and 17 days (five dogs) and then euthanatized and necropsied. tenesmus, dysuria, hematuria, and pollakiuria occurred in all dogs, but the severity of these signs diminished with time. the total white cell, neu ...19863773458
[the role of intrauterine contraceptive devices in the development of inflammatory processes in the small pelvis].the incidence of pelvic inflammatory disease (pid) attributable to iud use has been increasing, especially after the removal of the dalkon shield from the market, but this relationship has not been settled conclusively. in recent decades pid included a variety of infections, but lately the definition of pid has meant acute ascending infections of the female genital tract. its most common risk factors include promiscuity of iud use, although this can be reduced to one fourth by regular checkups ...19863763205
sniffing out the diagnosis. 19863756698
encephalocraniocutaneous lipomatosis and proteus syndrome. 19863777031
effect of radiation dose on the recovery of aerobic and anaerobic bacteria from mice.the presence of aerobic and anaerobic bacteria in the blood, spleen, and liver was investigated in mice that were exposed to 7, 8, 9, or 10 gy 60co radiation. microorganisms were detected more often in animals exposed to higher doses of radiation. the number of mice that were culture positive and the number of isolates in one site increased with increasing dose. bacteria were recovered in mice killed at various times after radiation, in 3 of 100 mice exposed to 7 gy, in 13 of 100 irradiated with ...19863779523
[frequency of isolation and drug resistance of bacterial strains from urine samples tested in the bacteriology and virology laboratory of the u.s.l. 10/d in florence].two thousand five hundred and sixty-eight positive monomicrobic urine samples (from general practice and hospital wards) were examined and isolated strains were tested against various antimicrobial drugs. our findings showed that urinary-tract infections caused by escherichia coli were the most common in females, whereas those by pseudomonas aeruginosa, proteus rettgeri, enterobacter species, other difficult gram-negative rods and streptococcus faecalis were in total the most common in males. if ...19863786667
computer-aided model-building strategies for protein design.model-building strategies for protein modification and design are developed. these strategies emphasize simple geometric aspects of protein structure, use local coordinate systems defined at particular residues, and systematically consider a large number of alternative sequences and conformations. we have written a computer program, proteus, to implement these search methods. proteus has been used to find positions where disulfide bonds could be added to the n-terminal domain of the lambda repre ...19863790501
sepsis and cholestasis: the in vitro effects of bacterial products on 14c-taurocholate uptake by isolated rat hepatocytes.bacterial endotoxins are known to be an important cause of cholestasis, yet not all organisms that cause cholestasis produce endotoxins. in order to determine whether bacterial products other than endotoxins may be involved in the cholestasis process, 14c-taurocholate (tc) uptake by isolated rat hepatocytes was measured in the presence of mid-log, stationary and mid-death phase bacterial broth supernatants from eight common bacterial pathogens. the results were then correlated with a quantitativ ...19863773649
antibiogram of urinary tract isolates in kuwait.1253 urinary tract isolates collected during 9 months (april-december 1984) were analysed by the agar diffusion method for sensitivity to 13 antimicrobial agents commonly used for the treatment of urinary tract infections in children and adults. we noticed an agitatingly high resistance of escherichia coli to ampicillin, sulphonamide and trimethoprim/sulphamethoxazole and of proteus spp. to ampicillin. as kuwait is one of the countries where the total consumption of antibiotics is very high as c ...19863775271
comparative "in vitro" activity of ciprofloxacin (bay o9867) and other antimicrobial agents against urinary bacterial isolates.ciprofloxacin (bay o9867) is a new quinolone derivative. its activity against 537 urinary bacterial isolates was compared with those of ampicillin, amikacin, carbenicillin, cefazolin, cefotaxime, cefoxitin, ceftazidime, nalidixic and oxolinic acids, norfloxacin, trimethoprim, and other antimicrobial agents, when appropriate. it inhibited 90% of citrobacter freundii, enterobacter spp., e. coli, klebsiella spp., proteus spp., and serratia spp. at 0.78 microgram/ml. the majority of pseudomonas aeru ...19863769047
in vitro antimicrobial activity in comparison with tonsillar tissue penetration of mezlocillin.the aim of this study was to determine the role of mezlocillin, a new acylureidopenicillin, in otorhinolaryngology. one hundred and forty-four bacterial strains were isolated from 100 patients suffering from infections of the oropharyngeal tract. among the isolates only 10 (6.9%) resulted in being mezlocillin-resistant by the agar diffusion method. minimum inhibitory and minimum bactericidal concentrations were also determined. pharmacokinetic parameters e.g., diffusion in tonsillar tissue and s ...19863802299
modulation of pulmonary defense mechanisms by acute exposures to nitrogen dioxide.the effect of acute exposures to no2 on the antibacterial defenses of the murine lung was assessed following inhalation challenges with staphylococcus aureus, proteus mirabilis, and pasteurella pneumotropica. animals were challenged with the bacteria and exposed for 4 hr to increasing concentrations of no2 after which pulmonary bactericidal activity was quantitated. with s. aureus pulmonary antibacterial defenses were suppressed at no2 levels of 4.0 ppm and greater. exposure to 10.0 ppm enhanced ...19873803338
resistance to antibiotics at medical centres in different parts of the world.the diameters of the zones of inhibition of consecutive clinical isolates around antibiotic susceptibility test discs at medical centres in different parts of the world were computer filed and analysed by a series of programs that evaluate test quality and compare results. percentages of isolates resistant to ampicillin at 18 centres ranged from 16 to 73 for escherichia coli and from 3 to 56 for proteus mirabilis. percentages resistant to chloramphenicol ranged from 2 to 48 for e. coli, from 5 t ...19863804896
oral ciprofloxacin therapy of infection caused by multiply resistant bacteria other than pseudomonas aeruginosa.of 125 patients treated with ciprofloxacin at the columbia-presbyterian medical center, new york, 34 had infections due to bacteria other than pseudomonas aeruginosa. the mean age of the patients was 50 years (19-88 years) and most had significant underlying disease. there were nine lower respiratory infections, eight urinary tract infections, eight soft tissue infections, three osteomyelitis, and three intra-abdominal infections. the pathogens were: escherichia coli, 7 (mean mic 0.07 mg/l); ser ...19863804906
two-directional pattern of movements on the cell surface of amoeba proteus.particles of latex, glass and precipitated alcian blue were studied cinematographically on the surface of migrating amoeba proteus and in the surrounding medium. the majority of the attached and all unattached particles flow steadily forward in the direction of the endoplasmic streaming and cell locomotion. flow on the surface is faster than in suspension. some particles stuck on the membrane move backwards from the frontal region. this retrograde transport is slower than the anterograde flow, a ...19863805143
[isolation and properties of aminocaprolactam hydrolase from providencia alcalifaciens].l-alpha-aminocaprolactam hydrolase possessing the l-lysinamidase activity was isolated and purified from providencia alcalifaciens. the purification procedure of enzymes included cell destruction on usdl-1, fractionation by ammonium sulfate, gel-chromatography on g-100, ion exchange chromatography on deae-cellulose. the purification resulted in a homogeneous enzyme which possessed the both activities. the enzyme molecular weight (180 kda) was estimated by gel chromatography on sephadex g-200. km ...19873810885
current state and tendencies of antibiotic resistance in hungary.this survey is based on data for 245 903 isolates reported by public health network laboratories in 1983. facultatively pathogenic gram-negative bacteria comprised two-third of the isolates, and--except escherichia coli--were resistant in a high percent to the most frequently used antibiotics. oxacillin and vancomycin were the most effective against staphylococcus aureus being in 94.7% resistant to penicillin. in contrast to other streptococci, all streptococcus pyogenes strains were sensitive t ...19863811828
effects of times of collection and different storage methods on the microflora and nitrogen content of poultry waste.an experiment was conducted to determine the effects of times of collection and storage methods on the kinds and numbers of microflora and nitrogen content of poultry excreta (waste). fresh waste collected in the morning had a higher mean count of viable bacteria than the evening collection in both strains of hen studied. fresh waste samples had higher mean counts than dried ones. escherichia coli and proteus spp. were the predominant microflora identified in waste collected at both times from b ...19863815127
cefonicid, a long acting cephalosporin: in vitro activity compared with three cephalosporins and gentamicin.minimum inhibitory concentrations of cefonicid, cefazolin, cefuroxime, ceftazidime and gentamicin were determined against 688 bacterial strains (234 gram-positive and 454 gram-negative) from 20 different genera. cefonicid showed a broader spectrum than cefazolin but was not as active as ceftazidime or gentamicin. cefonicid most closely resembled cefuroxime in terms of microbiological properties. disc testing accurately predicted sensitivity to cefonicid except for staphylococcus aureus (which wa ...19863816503
fatal nonclostridial gas gangrene in a diabetic: a case report and review of the literature. 19863798149
rapid catalase supplemental test for identification of members of the family enterobacteriaceae.a simple, rapid, semiquantitative slide catalase test useful for differentiating members of the family enterobacteriaceae is described. judging by the time required for appearance of oxygen bubbles in 3% hydrogen peroxide, the immediate catalase reactors were yersinia, serratia, proteus, morganella, providencia, cedecea, and hafnia spp. the delayed catalase reactors were escherichia, shigella, klebsiella, enterobacter, salmonella, citrobacter, edwardsiella, kluyvera, and tatumella spp. this info ...19873818934
response of bacterial antigen in palmoplantar pustulosis.motilities of leukocytes in response to bacterial antigens or sera were examined in tissues from patients with palmoplantar pustulosis (ppp). accelerated migration towards bacterial antigens was detected in the case of staphylococcus epidermidis in 9 of 19 patients, to propionibacterium acnes in 5 of 19, to proteus mirabilis and staphylococcus aureus in 3 of 19, while no acceleration was found in neutrophils from the controls. a significantly accelerated migration of normal lymphocytes in respon ...19863820277
[transfer of fosfomycin into human burn blister fluid and its pharmacokinetic analysis].fosfomycin (fom) (50 mg/kg) was administered to burned patients by intravenous bolus injection. burn blister fluid and serum were taken during 8 hours after injection, and concentrations of fom in burn blister fluid and serum were determined by bioassay using proteus sp. (mb-838) as the test organism. the serum concentrations of fom were 257 +/- 34.6 micrograms/ml at 15 minutes, 222 +/- 34.8 micrograms/ml at 30 minutes, 166 +/- 34.6 micrograms/ml at 1 hour, 114 +/- 43.9 micrograms/ml at 2 hours, ...19863820568
[urinary infections in patients in the u.s.l. 25 val di cornia. percentage frequency and sensitivity to antibiotics].in order to estimate the present situation concerning bacterial resistance to a group of widely-used antibiotics, the results of several antibiograms, which were carried out from october 1984 to february 1985 on bacterial stocks isolated from urinary cultures sent to the laboratory of clinical chemistry at the campiglia marittima hospital u.s.l. 25 (livorno) have been collected. the bacterial species most frequently observed are gram-negative, namely: escherichia coli (54%), proteus (19%), klebs ...19863823370
scombroid poisoning: mini-review with case histories.scombroid poisoning has become an almost world-wide medical problem. it is probably the most common cause of fish poisoning, although frequently misdiagnosed as "salmonella infection'. while there remains some question as to the definitive etiology, there is little doubt that the poisoning is caused by the ingestion of certain mackerel-like fishes whose tissues have undergone a number of changes provoked by bacteria, and involving the conversion of histidine to histamine, potentiated by diamines ...19863824404
endotoxemia and bacteremia in patients following ultrasonic lithotripsy.the incidence of endotoxemia was studied in 17 patients who underwent ultrasonic lithotripsy (usl), and compared with the incidence in 11 patients who underwent transurethral resection (tur) and in 8 patients who underwent other operations. fourteen of 17 patients (82%) who underwent ultrasonic lithotripsy had endotoxemia. the incidence of endotoxemia after usl was significantly higher than that after tur (9%) or after other operations (12.5%). only 2 of the 17 patients (12%) who underwent usl h ...19873824714
[a complex vaccine in the prevention of staphylococcal, pseudomonas aeruginosa and proteus infections in esophageal cancer patients].an analysis of clinico-immunological data has demonstrated a considerable decline in non-specific and specific defences against such major factors of hospital infection in esophageal cancer patients as staphylococcal, blue pus and proteus bacilli. immunization with a complex vaccine including concentrated staphylococcal anatoxin, blue pus and proteus vaccines was shown to stimulate the said factors of anti-infectious immunity and to be followed by a 4.7-fold decrease in the incidence of postoper ...19873825005
[in vitro activity of cefotaxime, latamoxef and ceftriaxone on hospital strains of enterobacteriaceae resistant to cefalotine and cefoxitine].an in vitro study of the sensitivity of three cephalosporins of the third generation was performed on 119 wild strains of enterobacteriae selected because of their resistance to cefalotine and cefoxitine. cefotaxime, latamoxef and cetriaxone remain very active on such strains since almost all of the strains were inhibited by a dose of 4 micrograms/ml and ci 50 p. cent was below 0.20 micrograms/ml. the activity of these three cephalosporins was comparable on groups e. coli, klebsiella-enterobacte ...19863826779
changes in resistance to aminoglycoside antibiotics of different bacterial strains isolated during the period 1978 to 1983. netilmicin as alternative therapy.the percentage resistance of 11,404 gram-negative and gram-positive bacterial strains isolated from different biological materials between 1978 and 1983 to some aminoglycoside antibiotics (gentamicin, tobramycin and amikacin) was determined. the percentage of resistant pseudomonas, staphylococcus and indole-positive proteus organisms significantly increased. there was also a significant decrease in gentamicin-resistant strains of the escherichia coli and proteus mirabilis species. we also compar ...19853830413
production of brownish pigment by bacteria of the morganella-proteus-providencia group.all bacteria of the morganella-proteus-providencia group tested produced a brownish pigment on agar containing l-forms of aromatic amino acids, i.e. histidine, phenylalanine, tryptophan, and tyrosine in the presence of iron ions under aerobic conditions. no pigment was observed without iron or when using d-forms of the amino acids. it is a melanin-like pigment with variable molecular weight under about 12000 d and an absorbance maximum between 330 and 410 nm depending on the concentration and th ...19853834717
depression of delayed hypersensitivity responses in patients with pertussis.components of bordetella pertussis cause impairment of cell-mediated immunity in experimental animals and children with acute pertussis have been shown to have a reduced prevalence of positive tuberculin skin tests (13). furthermore, secondary infection is one of the major causes of morbidity and mortality in this disease. on the basis of these observations, we have studied delayed hypersensitivity responses in children with b. pertussis infection and compared the results with responses elicited ...19853835078
[the incidence and antibiotic resistance of bacteria of the proteus genus in various clinical materials from regional hospitals of perugia (1970-1981)]. 19853836398
[gram-negative bacteria resistant to antibiotics in foods].from 154 food samples, including vegetables (lettuce), milk and meals served at school it was possible to isolate and identify 400 gram negative bacilli distributed among 339 enteric bacteria (escherichia, shigella, citrobacter, klebsiella, enterobacter, serratia and proteus) and other 61 non enteric bacilli (acinetobacter, flavobacterium, aeromonas and pseudomonas). submitting this cultures to the drugs sulfadiazine (su), streptomycin (sm), tetracycline (tc), chloramphenicol (cm), kanamycin (km ...19853837834
the effect of the combination of erythromycin with new beta-lactam antibiotics against gram-negative aerobic respiratory pathogens.the effect of erythromycin on the in vitro activity of cefotaxime, ceftizoxime, cefoperazone, moxalactam, ceftazidime, cefmenoxime, aztreonam, imipenem, piperacillin, and gentamicin against 89 bacteria isolated from sputum and tracheal aspirates of patients admitted to intensive care units was evaluated. there were 30 pseudomonas aeruginosa, 27 klebsiella pneumoniae, 9 enterobacter cloacae, 5 escherichia coli, 4 enterobacter aerogenes, 3 each of klebsiella oxytoca, pseudomonas maltophilia, serra ...19863802298
use of aztreonam in patients suffering from urinary tract infections with contraindications for the use of aminoglycosides.the clinical efficacy and safety of aztreonam were studied in 30 patients suffering from urinary tract infections caused by gram-negative bacteria. aztreonam was given in a dose of 1 g i.m. at intervals of 12 or 24 hours. duration of therapy was 2-8 days. bacteria isolated in urine cultures were: escherichia coli (60%); proteus mirabilis (10%); pseudomonas aeruginosa and citrobacter sp. (7%); proteus vulgaris, serratia marcescens; klebsiella pneumoniae and pseudomonas species (3%). at completion ...19863791478
[clinico-pathogenetic basis for using crystalline lysozyme in the combined therapy of food toxinfections].in food toxinfections caused by various microorganisms (staphylococcus, escherichia, klebsiella, proteus, citrobacter, etc.) a decrease of lysozyme debit and an increase of ph of gastric juice were found. one third of patients exhibited lactose deficiency of the small intestine. treatment with furazolidone contributed to the development of lactase deficit and delayed stools normalization. crystalline lysozyme shortened duration of febrile reaction and diarrhea, its intake facilitated lactose hyd ...19863817153
[6r-[(r)-2-[3-[2-(p-aminosulfonyl)anilino-4-hydroxy-5- pyrimidinyl]ureido]-2-(4-hydroxyphenyl)acetamido]-penicillanic acid, sodium salt (vx-vc 43), a new broad-spectrum beta-lactam antibiotic. in vitro and in vivo antibiotic activity].6r-[(r)-2-[3-[2-(p-aminosulfonyl) anilino-4-hydroxy-5-pyrimidinyl] ureido]-2-(4-hydroxyphenyl)acetamido]-penicillanic acid, sodium salt (vx-vc 43), a pyrimidinylureidopenicillin, is a broad-spectrum antibiotic. in vitro it is more active than piperacillin, apalcillin and mezlocillin especially against the following strains; e. coli, ps. aeruginosa, ps. stutzeri, k. pneumoniae, eb. cloacae, ser. marcescens, proteus, sh. flexneri, y. enterocolitica, cb. freundii, acb. calcoaceticus, bacteroides an ...19853838677
acute rickettsial myocarditis and advanced atrioventricular block: diagnosis and treatment aided by endomyocardial biopsy.a case of acute advanced atrioventricular block in a young patient is described. an endomyocardial biopsy performed to confirm myocarditis showed findings compatible with rickettsial endomyocarditis. treatment with tetracycline was therefore started and a rapid remission of the atrioventricular block was observed. healing of the disease was subsequently documented by a second endomyocardial biopsy. the rickettsial etiology was confirmed by the results of serial serum titers against proteus ox-19 ...19853838736
in vitro activity of bmy-28142 in comparison with those of other beta-lactam antimicrobial agents.the in vitro susceptibility of 406 clinical isolates to bmy-28142, a new semisynthetic cephem, was evaluated and compared with cefpimizole, hr 810, ceftazidime, cefotaxime, moxalactam, and cefoperazone in a broth microdilution assay. on a weight basis, the activity of bmy-28142 against escherichia coli, proteus species, and klebsiella-enterobacter-serratia was superior to the other cephems. against gentamicin-susceptible and -resistant pseudomonas aeruginosa, bmy-28142 was more active than the o ...19853839120
comparative in vitro and in vivo activity of cefminox (mt-141), cefotaxime and cefoperazone against gram-negative bacteria.against 17 gram-negative bacteria, excluding proteus species, the difference between cefminox and cefotaxime was significant in terms of mic distribution, in favour of cefotaxime, but was not significant in terms of ed50 distribution. against 22 proteus species, the difference between cefminox and cefotaxime was not significant in terms of mic distribution, but was significant for ed50 distribution, in favour of cefminox. the difference between cefminox and cefoperazone was not significant for e ...19853841789
[clinical evaluation of cefmenoxime in chronic complicated urinary tract infection].the efficacy of cefmenoxime (cmx), which is a third generation, beta-lactamase-resistant cephem with a broad antibacterial spectrum, was examined in 43 patients with chronic complicated urinary tract infections. the usual dosage regimen was given 2 approximately 4 g/day of cmx by intravenous drip infusion over 1 hour. the duration of treatment was 5 days. fifteen patients were cured and 21 improved, and the effective rate was 83.7%. bacterial eradication rate in these cases was 88.2%, especially ...19853857363
[microbiologic and clinical significance of cefotiam].modern cephalosporins are of considerable importance for the therapy of severe infections by multiresistant organisms. according to in-vitro-findings on ampicillin-resistant e. coli as well as klebsiella spp., proteus spp., and serratia spp., altogether 159 strains, instead of cefotaxime nearly always also cefotiam can be used. the two remedies are clearly superior to cephalothin. cefotiam is ineffective to pseudomonas aeruginosa. but in this case also cefotaxime is clearly inferior to azlocilli ...19853857792
impact of imipenem/cilastatin therapy on normal fecal flora.the impact of parenteral imipenem/cilastatin therapy on the bowel flora of six patients was evaluated. stool samples were collected before and during therapy and qualitative and quantitative bacteriologic studies were performed. imipenem had no effect on total microorganism counts. two patients acquired candida albicans during therapy, and three patients acquired proteus species. pseudomonas species in one patient acquired resistance. imipenem appears to have a relatively modest effect on the bo ...19853859214
[experimental and clinical studies on cefmetazole in the field of internal medicine].susceptibilities in various types of clinical isolates to cefmetazole (cmz) were determined by the disk method and the serial agar dilution method for mic measurement. cmz showed high antibacterial activity for all gram-positive cocci except e. faecalis, and for h. influenzae, e. coli, klebsiella sp. and proteus sp.. cmz was administered on 33 patients with infections, mainly biliary tract infections, in the field of internal medicine. the clinical efficacy of the drug was 86.7% for infections o ...19853859674
[cefotiam concentration in exudates from the drainage of patients with acute peritonitis following intravenous administration].cefotiam dihydrochloride (ctm) in a dose of 2 g was given by intravenous bolus injection to 15 patients operated upon for acute or subacute abdominal organs. they were included to 5 cases of acute localized peritonitis due to perforative appendicitis, 3 of diffuse peritonitis due to perforative duodenal ulcer, 1 of diffuse peritonitis with intestinal obstruction, 1 of perforative sigmoid colon cancer, 2 of gastric cancer or polyp with cholelithiasis, and 3 of cholangitis with cholecyst-choledoch ...19853862880
influence of imipenem on the serum resistance of enterobacteriaceae.following growth in a subinhibitory concentration of imipenem and additional incubation in a 20% dilution of normal human serum (nhs) for 90 minutes, five of 12 serum-resistant strains of enterobacteriaceae showed a decrease in colony-forming units of two or more logs of growth compared with the control. two strains (of escherichia coli and enterobacter aerogenes) showed this phenomenon even with incubation in 5% nhs. treatment with imipenem did not change the serum resistance of the other seven ...19853863217
[infectious complications and infection prevention in patients with acute leukemia].febrile episodes in 147 patients undergoing first remission induction therapy for acute leukemia were analyzed. febrile episodes occurred 254 times in 136 patients. the cause of fever could not be identified in 54.3% of all episodes. antibiotic therapy was effective in 81.1% of these episodes with no cause determined. postmortem examinations proved infections in 65.2% of patients who had fever of unknown origin before death. sepsis and pneumonia together accounted for 53.4% of documented infecti ...19853863970
in-vitro activity of ofloxacin, a quinolone carboxylic acid compared to other quinolones and other antimicrobial agents.ofloxacin is a new quinolone carboxylic acid compound. its activity against 900 bacterial isolates was determined. it inhibited 90% of escherichia coli, klebsiella sp., aeromonas hydrophila, salmonella spp., shigella spp., citrobacter spp., enterobacter spp., morganella morganii, proteus mirabilis, yersinia enterocolitica at less than or equal to 0.8 mg/l. branhamella catarrhalis, haemophilus sp., neisseria sp. were inhibited by less than or equal to 0.1 mg/l. pseudomonas aeruginosa and other ps ...19853865923
fine needle aspiration biopsy cytology in the diagnosis of inflammatory lesions.in 42 fine needle aspirations (fna), the cytologic findings were interpreted as either suppurative or granulomatous inflammation. the majority of these fnas were performed in patients with a known history of malignancy in whom recurrent or residual malignancy was suspected clinically. in 13 cases, a specific microbiologic diagnosis was made on the basis of the aspirate, either by cytology or by culture. in the remaining 29 cases, no specific diagnosis was possible. open biopsies were later perfo ...19853866462
[anti-bacterial effect of ofloxacin--in vitro and in vivo results].gram (-) bacteria such as e. coli, b. proteus, klebsiella and pseudomonas sp were found to be sensitive to ofloxacin 98.3%, 96.7%, 96.9%, and 89.1% respectively. among gram (+) bacteria, 98% of staph aureus and 95% of staph epidermidis were susceptible to this drug. the minimum inhibitory concentrations of ofloxacin for the shigella strains have been found to be between 0.1-0.05 mcg/ml (76%) and 0.05-0.025 mcg/ml (20%). thirty three patients with various infection have been treated with ofloxaci ...19853868736
[therapeutic efficacies of suppository of ceftizoxime against experimental infections in mice].in experimental infections in mice, the therapeutic efficacies of rectal administration of ceftizoxime (czx) were compared with those of subcutaneous administration. the efficacies of rectal administration were equivalent to those of subcutaneous administration against intraperitoneal infections due to streptococcus pneumoniae and escherichia coli. against staphylococcus aureus, streptococcus pyogenes, klebsiella pneumoniae, enterobacter cloacae, proteus mirabilis, morganella morganii and serrat ...19853869265
conversion of cloxacillin into a progressive inhibitor of beta-lactamases by sulfonation and its activity against various types of these enzymes.on the assumption of the theory that the sulfone of a penam derivative should effectively act as a suicide substrate against beta-lactamases to which the parent compound is a poor substrate, the action of cloxacillin sulfone on four different types of beta-lactamases was studied. as we expected, cloxacillin sulfone showed strong mechanism-based irreversible inactivation against type ib penicillinase and proteus vulgaris cephalosporinase whereas it showed no progressive inactivation against cloxa ...19853871761
comparative in vitro activity of ceftriaxone against clinical bacterial isolates in nigeria.the in vitro activity of ceftriaxone, a cephalosporin derivative, recently introduced in nigeria was compared with the activities of other related beta-lactam antibiotics such as cefotaxime, cefuroxime, cephaloridine, cloxacillin/ampicillin and ampicillin against 530 local clinical isolates of gram-negative and gram-positive bacteria. the spectra of activity and potency of ceftriaxone and cefotaxime were generally similar against the enterobacteriaceae, pseudomonas aeruginosa, serratia spp., str ...19853872779
specific inhibition of urease by n-acylphosphoric triamides.benzoyl- and isopentenoyl phosphoric triamides (bpa and ipa) strongly inhibited urease activities from jack bean, soybean, watermelon seed, proteus mirabilis, p. rettgeri, p. vulgaris, mycobacterium smegmatis, and ureaplasma urealyticum. their i50 values (the final concentration causing 50% inhibition), independent of enzyme source, were 2-21 nm, which are about 1,000-fold lower than that of caprylohydroxamic acid, one of the most potent urease inhibitors. atp-urea amidolyase activity was inhibi ...19853841542
timentin in the treatment of symptomatic complicated urinary tract infections in adult patients.the safety and effectiveness of timentin were evaluated in 34 adult patients with symptomatic complicated urinary tract infections, principally due to multiply-drug-resistant bacteria. although a wide variety of organisms, particularly gram-negative bacilli, were found, escherichia coli was the most frequent, accounting for 14 of 45 (31 percent) pathogens isolated. ten (22 percent) isolates were pseudomonas aeruginosa; 11 (24 percent) were proteus or morganella species; three (7 percent) were ci ...19853852637
effects of antimicrobial agents fed to chickens on some gram-negative enteric bacilli.total and antimicrobial agent-resistant aerobic and facultative anaerobic gram-negative enteric bacilli in fecal samples of broiler chickens fed growth-promotional levels of antimicrobial agents were determined quantitatively. two 8-week studies were conducted utilizing groups of chickens fed antimicrobial-supplemented rations; the second study involved feed "pasteurization" as a means of minimizing colonization from the feed. dilution/spread-plating/replica-plating techniques on selective media ...19853852666
post-operative bacterial endophthalmitis.this paper critically reviews current practice in the united kingdom in the presence of post-operative bacterial endophthalmitis. an appropriate therapeutic regime is suggested and the value of vitrectomy is emphasised together with the importance of the administration of intra-vitreal and intra-venous antibiotics.19853875169
in-vitro activity and beta-lactamase stability and inhibitory properties of a new penem antibiotic, sch 34343.sch 34343 is a new penem antibiotic. its in-vitro activity was determined against gram-positive and -negative aerobic and anaerobic isolates. sch 34343 inhibited 90% of strains of escherichia coli, klebsiella pneumoniae, proteus mirabilis, salmonella spp, citrobacter spp. and shigella spp. at less than 1 mg/1. ninety per cent of strains of enterobacter, morganella and providencia spp. were inhibited by less than 4 mg/1. sch 34343 did not inhibit pseudomonas spp., and it had slightly less activit ...19853875603
ceftriaxone against gram-negative and gram-positive bacteria: bactericidal and post-antibiotic effect.the effect of ceftriaxone against gram-negative and gram-positive strains of different clinical isolates has been compared to that of other cephalosporins such as cefuroxime, cefoxitin and cefotaxime. cefotaxime and ceftriaxone have a notably higher activity against enterobacteriaceae than cefuroxime and cefoxitin. if ceftriaxone activity is compared to cefotaxime activity, it may be seen that the former shows a stronger action against pseudomonas, proteus rettgeri, enterobacter and serratia. ag ...19853875430
multiple antibiotic resistance plasmids in enterobacteriaceae isolated from diarrhoeal specimens of hospitalized children in indonesia.we studied the plasmid and antibiotic resistance characteristics of 35 strains of enterobacteriaceae recovered from faecal specimens of children with diarrhoea in central general hospital, bandung, indonesia. twenty three escherichia coli, three providencia, three proteus, three klebsiella, two enterobacter and one citrobacter were examined. all strains were multiply resistant, many carrying six to nine antibiotic resistances. most of these resistances were transferable to a laboratory e. coli s ...19853876328
disease patterns in the detroit zoo: a study of the avian population from 1973 through 1983.a retrospective study was conducted to evaluate disease patterns in birds at the detroit zoo from 1973 through 1983. data were derived from the zoo's medical and animal census records; the mean (+/- sd) population of birds during the study period was 469 +/- 42. overall annual morbidity rates were 12.5% to 21.5%, with spring months having the highest morbidity rates. annual mortality rates were 3.1% to 15.2%; 23.9% of the deaths were caused by microbial agents (particularly erysipelothrix rhusio ...19853878351
disease patterns in the detroit zoo: a study of reptilian and amphibian populations from 1973 through 1983.a retrospective study was conducted to determine disease patterns in reptilian and amphibian populations at the detroit zoo from 1973 through 1983. in the reptilian population (mean +/- sd = 285.2 +/- 28), overall annual mortality rates were 1% to 40%. mortality rates were highest in the fall months (20%) and lowest in the winter months (6%). the most frequently affected reptiles were iguana (iguana iguana), reticulated python (python reticulatus), rattlesnakes (crotalus spp), common boa (constr ...19853878352
in-vitro activity of the combinations of ampicillin with mecillinam or with beta-lactamase inhibitors against strains resistant to ampicillin.the in-vitro activity of ampicillin, of mecillinam and of combinations of ampicillin with mecillinam, clavulanic acid or 6 beta-bromopenicillanic acid has been studied against 126 enterobacteriaceae resistant to ampicillin. the combination of ampicillin with mecillinam showed synergy or addition in 60% of the combinations tested. synergy was seen especially when the strains were resistant to mecillinam, indifference when they were susceptible to mecillinam. the combination of ampicillin with mec ...19853879248
the activity of cefbuperazone, a 7 alpha-methoxy 7 beta acyl ureido cephalosporin.the activity of cefbuperazone, a 7 alpha-methoxy ureido cephalosporin, was determined against 726 clinical isolates. ninety percent of escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, citrobacter diversus, proteus mirabilis, enterobacter aerogenes, proteus vulgaris, morganella morganii, salmonella, and shigella species were inhibited by less than or equal to 6.3 micrograms/ml. cefbuperazone was more active than cefamandole, cefoxitin and piperacillin against these species. concentrat ...19853879464
[comparative activity of norfloxacin and other chemotherapeutic agents on the adhesion of bacteria of the uro-vaginal tract to uroepithelial cells].a number of bacterial strains (escherichia coli, proteus mirabilis and streptococci), obtained from patients with urinary infections, or from vaginal tampons, were assayed in order to evaluate their ability to adhere to uroepithelial cells. we employed, at a comparative purpose, two different methods to eliminate unattached bacteria: the centrifugation technique and the filtration technique. the effect of the pretreatment with subinhibitory concentrations of three chemotherapeutics widely employ ...19853879893
isolation of altered reca polypeptides and interaction with atp and dna.in this paper we describe the partial proteolytic digestion of reca proteins from escherichia coli and proteus mirabilis and the production and isolation of truncated reca polypeptides. a proteolytic fragment of the p. mirabilis reca protein bound single-strand dna and atp normally but has altered duplex dna binding properties. this protein was shown to initiate but not complete dna strand transfer from a dna duplex to a complementary single strand. the product of the e. coli reca1 allele bound ...19853881430
biochemical identification of new species and biogroups of enterobacteriaceae isolated from clinical specimens.in 1972 there were only 11 genera and 26 species in the family enterobacteriaceae. today there are 22 genera, 69 species, and 29 biogroups or enteric groups. this paper is a review of all of the new organisms. it has a series of differential charts to assist in identification and a large chart with the reactions of 98 different organisms for 47 tests often used in identification. a simplified version of this chart gives the most common species and tests most often used for identification. the so ...19853881471
uropathogenic properties of proteus mirabilis and proteus vulgaris.a group of faecal isolates of proteus vulgaris and p. mirabilis was studied for the presence of possible virulence factors such as growth rates in urine and broth, haemolysin production, hydrophobicity, sensitivity to the bactericidal activity of human serum and cell invasiveness. differences were found in haemolysin production, cell invasiveness and experimental virulence in a mouse model. these differences might explain why p. mirabilis is much more common in human urinary-tract infections tha ...19853881591
aztreonam in the treatment of serious orthopedic infections.aztreozam was evaluated in the treatment of a variety of orthopedic infections. included were 17 patients with osteomyelitis, three with purulent arthropathy with prostheses, and 16 with superficial infections secondary to trauma or surgical procedure. pathogens were gram-negative bacilli sensitive to aztreonam. concomitant antibiotics were administered for gram-positive cocci that were present initially or by superinfection. infecting organisms included pseudomonas aeruginosa (minimal inhibitor ...19853881949
[gentamicin: various pharmacotherapeutic aspects in comparison with other aminoglycosides].gentamicin may be used in the treatment of infection with gram-negative bacteria including pseudomonas spp and proteus spp. resistance will only appear in suboptimal or too prolonged courses of treatment and usually is due to 'multi-step mutation'. this resistance may be prevented, among others, by combined treatment with gentamicin and an antibiotic of the beta lactam group. when gentamicin is used correctly, it will have few toxic side-effects. thus, 3 mg/kg of body weight three times daily wi ...19853883571
auto-antibody to kidney tubular cells during retrograde chronic pyelonephritis in rats.retrograde pyelonephritis was induced in inbred fischer rat kidneys with proteus mirabilis (1 x 10(8) organisms/ml). sera from pyelonephritic animals sacrificed at 4 and 6 weeks contained cytotoxic antibodies to cultured syngeneic 51cr-labeled kidney tubular cells (p less than 0.02 and p less than 0.05, respectively) which could be absorbed with plasma membranes. immune sera from 4- and 6-week pyelonephritic animals also displayed a granular fluorescent pattern along the surface of cultured kidn ...19853885065
pneumatocele formation in a patient with proteus mirabilis pneumonia.a rare case of pulmonary pneumatoceles complicating the course of proteus pneumonia in an adult is described. the pneumatoceles which appeared early in the course of the pneumonia resolved completely without complications. a brief review of the literature regarding formation of cavities in proteus pneumonia is given.19853885205
[indicator importance of the bacterial genus proteus in assessing the hygienic status of food products]. 19853885570
primary psoas abscess three years after ipsilateral nephrectomy.we report an unusual case of primary, right psoas abscess caused by proteus mirabilis which developed three years after ipsilateral nephrectomy. the diagnosis was established by computer tomography, recommended today as superior to other diagnostic methods of demonstration for the retroperitoneum.19853886552
pathogenesis of escherichia coli cystitis and pyelonephritis: apparent lack of significance of bacterial motility and chemotaxis towards human urine.all 45 microscopically motile urinary isolates tested here (37 escherichia coli, two enterobacter cloacae, two citrobacter freundii, three proteus mirabilis and one proteus morganii) were strongly attracted to fresh human urine in a capillary chemotaxis assay. this observation suggested that urine taxis of gram-negative bacteria promotes their invasion of the human lower urinary tract and their ascension to the kidney(s). however, the incidence of motile isolates and their activity in urine taxi ...19853886554
unusual nasal foreign body in a dog.a metallic hunting arrowhead was found in the nasal sinuses of a dog with chronic nasal discharge. the arrowhead was removed via rhinotomy. nasal discharge ceased after the foreign body's removal.19853886614
hemorrhagic bullae associated with morganella morganii septicemia.to our knowledge this is the first case of hemorrhagic bullae caused by morganella morganii septicemia. the presence of organisms in the bullae, demonstrated by gram strain and culture, and the acral location of the bullae suggest that the skin lesions were due directly to blood-borne infection. multiple factors predisposed this patient to gram-negative bacteremia, including lymphoma, chemotherapy, neutropenia, systemic steroids, multiple hospitalizations, and treatment with broad-spectrum antib ...19853886721
in vitro evaluation of current disinfectants for leg bags.an in vitro study was performed investigating the bactericidal ability of five common cleaning agents for leg bags against organisms frequently causing urinary tract infection in spinal cord injured (sci) patients. individual disposable uridrain leg bags were inoculated with urine containing greater than 10(5) colonies/ml of the selected organism. the organisms used were: pseudomonas aeruginosa, proteus mirabilis, providencia stuartii, klebsiella pneumoniae, serratia marcescens, herellea, and en ...19853886841
[antibacterial activity of cefmenoxime against clinical isolates. comparative study].antibacterial activity of cefmenoxime (cmx) against clinically isolated organisms was examined in comparison with that of 4 other antibiotics and concluded as follows: antibacterial activity of cmx was markedly stronger than those of cefazolin (cez), cefmetazole, latamoxef and ampicillin against e. coli, k. pneumoniae, s. marcescens, h. influenzae, p. mirabilis and indole positive proteus. but the mic level of cmx against s. aureus was higher than that of cez.19853886949
the pleiotropic properties of a beta-lactam antibiotic-hypersensitive mutant derived from proteus vulgaris ifo 3167. 19853887108
the in vitro activity and beta-lactamase stability of cefpiramide compared with other beta-lactam antibiotics.the in vitro activity of cefpiramide, a new ureido cephalosporin antibiotic, was determined against 1128 gram-positive and gram-negative aerobic and anaerobic bacteria selected for resistance to ampicillin and cefazolin. cefpiramide was less active on a milligram basis than cefotaxime, ceftazidime, moxalactam, and aztreonam against all of the members of the enterobacteriaceae. cefpiramide inhibited many pseudomonas aeruginosa resistant to carbenicillin, piperacillin, and cefotaxime, but it was l ...19853877607
antibacterial activity of ticarcillin in the presence of clavulanate potassium.the antibacterial effects produced by ticarcillin disodium plus clavulanate potassium, a combination of the broad-spectrum penicillin ticarcillin, and the beta-lactamase inhibitor clavulanic acid as the potassium salt, have been measured in vitro and in experimental infection studies. the presence of clavulanic acid resulted in a significant enhancement of the activity of ticarcillin against a wide range of beta-lactamase-producing bacteria. these included ticarcillin-resistant strains of escher ...19853878080
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