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murein biosynthesis in synchronized cells of proteus mirabilis. quantitative analysis of o-acetylated murein subunits and of chain terminators incorporated into the sacculus during the cell cycle.cells of proteus mirabilis, synchronized by sucrose density gradient centrifugation, were grown in complex medium containing radioactive n-acetylglucosamine. at various times, labelled murein sacculi were isolated and digested with endo-n,o-acetylmuramidase from chalaropsis. the murein fragments thus obtained were separated into disaccharide peptides as the monomeric subunits and into peptide-cross-linked subunits by gel filtration. the subunits were further differentiated into o-acetylated and ...19873545834
diaper area skin microflora of normal children and children with atopic dermatitis.in vitro studies established that neither cloth nor disposable diapers demonstrably contributed to the growth of escherichia coli, proteus vulgaris, staphylococcus aureus, or candida albicans when urine was present as a growth medium. in a clinical study of 166 children, the microbial skin flora of children with atopic dermatitis was compared with the flora of children with normal skin to determine the influence of diaper type. no biologically significant differences were detected between groups ...19873546360
a two-center review of bacteremia in the community hospital.there are fewer studies on bacteremia coming from the community hospital, where the practicing family physician is likely to see this problem, than from the university hospital. the hypothesis of this study was that patterns of bacteremia would be different between the two types of hospitals. two hundred four patient episodes of culture-proven bacteremia from two analogous community hospitals were reviewed. bacteremia was discovered in 2.6 of 1,000 patients, which is lower than reports from univ ...19873546587
role of internal fixation in infection of open fractures: studies with staphylococcus aureus and proteus mirabilis.studies of infections in open fractures are described. the hamster was chosen as the experimental model. osteotomies of the femur were created with an osteotomy saw. some osteotomies were left to heal and others were fixed with an 0.9 mm k-wire. the infection rates in fixed and unfixed fractures were compared. the first group of hamsters with fixed and unfixed osteotomies was returned to cages with open wounds. there was no difference in the infection rate at 2 weeks. the second group was delibe ...19873546643
considerations in the choice of sutures for wound closure of the genitourinary tract.the ideal suture for use within the urinary tract should maintain its tensile strength until wound repair is satisfactory and then it should undergo rapid total absorption without promoting stone formation. the bladder has a great potential for repair, attaining 100 per cent of the strength of the unwounded tissue in 14 to 21 days. braided synthetic absorbable sutures appear to be suited ideally for closure of incised wounds of a urinary conduit. they maintain their tensile strength for approxim ...19873546730
[isolation of thymidine-dependent strains. microbiologic considerations and personal observations].we describe the emergence of three thymidine-dependent strains (thy-): two escherichia coli obtained in successive occasions from urine of a woman treated with co-trimoxazole before the organisms were isolated and one proteus mirabilis obtained from urine of a man who had a treatment with norfloxacin. cultural characteristics and methods for the detection of these thy- strains are given.19863547456
microbiology of the female genital tract.patients who contract genital tract infections are predominantly young, are otherwise healthy, and generally respond well to treatment for bacterial infections. these infections are most commonly polymicrobial in etiology, with several noteworthy exceptions. often there is an inciting event such as childbirth, surgical intervention, pregnancy termination or intrauterine contraceptive device insertion. with treatment, prognosis for cure is excellent; however, sequelae such as recurrent infections ...19873548376
buoyant density variation during the cell cycle in microorganisms.the behavior of cell buoyant density during the cell cycle has been determined for a number of different cell types, including bacteria, yeast, and mammalian cells. mean buoyant density was extremely constant and independent of cell age during the cell cycle of the bacterium escherichia coli, the fission yeast schizosaccharomyces cerevisiae, the protozoan amoebae proteus, cells from suspension cell cultures of mouse lymphoma and myeloma, and chinese hamster ovary cells. in all of these cases, th ...19873549158
inducible expression of an aminoglycoside-acetylating enzyme in providencia stuartii.strains of providencia stuartii were collected from a chronic-care geriatric ward of a large veteran's administration hospital. two strains of p. stuartii, and one of escherichia coli isolated from the same ward, were transferred five times in the presence of gentamicin or netilmicin. at the fifth transfer the mics of both gentamicin and netilmicin for the two p. stuartii strains had increased at least ten-fold. the mics for the e. coli remained essentially unchanged. enzyme assay demonstrated i ...19873549668
the secreted hemolysins of proteus mirabilis, proteus vulgaris, and morganella morganii are genetically related to each other and to the alpha-hemolysin of escherichia coli.secreted hemolysins were extremely common among clinical isolates of proteus mirabilis, proteus vulgaris, and morganella morganii, and hemolytic activity was either cell associated or cell free. southern hybridization of total dna from hemolytic isolates to cloned regions of the escherichia coli alpha-hemolysin (hly) determinant showed clear but incomplete homology between genes encoding production of hemolysins in the four species. one of the two e. coli secretion genes, hlyd, hybridized only w ...19873549692
adherence of escherichia coli and proteus mirabilis to human transitional cells.this study utilizes a light microscopy assay for bacterial adherence to human male transitional cells. prior light microscopy studies have used voided squamous cells, periurethral cells or scraped vaginal cells, which are less representative of the cells lining the majority of the urinary tract. using a modification of previous bacterial adherence assays, the mean adherence for 28 strains of e. coli in 92 bacteria-cell incubations was 10.2 +/- 11.5 (standard deviation) bacteria per cell. the mea ...19873550157
[ultrastructural characteristics of proteus vulgaris and proteus mirabilis cells differing in the capacity for swarming].specific differences in the structure of colonies and the location of microbial cells in colonies, characteristic for aggregating and nonaggregating genetically related pairs of p. vulgaris and p. mirabilis strains, have been demonstrated by means of transmission and scanning electron microscopy. in calculating the number of flagellae per 100 outlines of microbial bodies revealed in negatively stained preparations, the fact that both aggregating and nonaggregating bacteria possess practically th ...19873551413
[experimental study of the immunological activity of a preparation made from the antigenic complexes of opportunistic microorganisms administered by different routes].the data obtained in the experimental study of the humoral factors of local and systemic immunity, as well as the morphofunctional changes of internal organs, after multiple subcutaneous and aerosol immunization with the combined preparation of proteus, staphylococcus, klebsiella pneumoniae and escherichia coli antigens (preparation nc-4) are presented. the subcutaneous and aerosol administration of preparation nc-4 has been found to induce an increase in the levels of antibodies to all componen ...19873551416
failure to detect hydrogen-sulphide production in lactose/sucrose-fermenting enterobacteriaceae, using triple sugar iron agar.triple sugar iron agar failed to detect hydrogen sulphide in 44 out of 69 hydrogen-sulphide producing strains of enterobacteriaceae, which at the same time fermented lactose and/or sucrose. the species involved were salmonella typhi, salmonella enteritidis, citrobacter freundii, escherichia coli, and proteus vulgaris. by contrast, no false-negative reactions were observed in 74 strains, which fermented neither lactose nor sucrose. failure to detect hydrogen sulphide was probably due to acidifica ...19873551500
five-year survey of changing patterns of susceptibility of bacterial uropathogens to trimethoprim-sulfamethoxazole and other antimicrobial agents.we analyzed the antibiotic susceptibility of 5,348 urinary isolates of escherichia coli, "klebsiella aerogenes," and proteus mirabilis grown in three laboratories from 1980 to 1985. a continuous rise in resistance to trimethoprim-sulfamethoxazole was observed; 63% of the strains from inpatients in 1984 and 51% of those from outpatients in 1985 were resistant to this drug. isolates from outpatients in 1985 were mostly susceptible to nitrofurantoin (mean susceptibility, 92%) and to oral cephalospo ...19873551824
in vivo evaluation of tigemonam, a novel oral monobactam.tigemonam, a new monobactam with excellent activity against gram-negative bacteria, was evaluated for in vivo efficacy and absorption after oral administration to laboratory animals. tigemonam is absorbed when administered orally to mice and dogs. in a variety of gram-negative systemic infections in mice, orally administered tigemonam was efficacious in all infections studied. comparison drugs such as amoxicillin, cephalexin, and cefaclor were less efficacious, especially in infections caused by ...19873551830
inducing capacity of the combinations mecillinam-ampicillin and mecillinam-ceftazidime in comparison with the capacity of the compounds administered separately.the inducing capacity of mecillinam in combination with ampicillin or ceftazidime was compared with that of the compounds administered separately and related to the inducing capacity of cefoxitin (1 and 10 micrograms/ml) for the chromosomal beta-lactamases from enterobacter cloacae, serratia marcescens, citrobacter freundii, indole-positive proteus, and acinetobacter strains. in the majority of the strains all compounds tested alone or in combination showed a lower inducing capacity than cefoxit ...19873552473
effect of fosfomycin trometamol on bacterial adhesion in comparison with other chemotherapeutic agents.the effect of fosfomycin trometamol in comparison with that of norfloxacin and co-trimoxazole on the hemagglutination, yeast cell aggregation and adhesive properties of both gram-positive and gram-negative microorganisms was studied. the strains were isolated from the urine of patients with urinary tract infections, or from vaginal swabs. at sublethal concentrations (1/4 and 1/8 of the minimal inhibitory concentration), the ability of the bacteria to adhere to human uroepithelial cells was reduc ...19873552703
spectrum of bacterial pathogens in uncomplicated and complicated urinary tract infections.the spectra of bacterial organisms of urinary tract infections (uti) in patients with and without abnormalities in the urinary tract (complicated and uncomplicated utis) are compared. data from the united kingdom and the federal republic of germany are included. in addition, the susceptibility of the pathogens to antibiotics (for oral or parenteral application) in both countries are compared. in conclusion, rational antibiotic therapy of complicated utis should strictly follow the antibiogram of ...19873552704
purification and characterization of the nickel-containing multicomponent urease from klebsiella aerogenes.klebsiella aerogenes urease was purified 1,070-fold with a 25% yield by a simple procedure involving deae-sepharose, phenyl-sepharose, mono q, and superose 6 chromatographies. the enzyme preparation was comprised of three polypeptides with estimated mr = 72,000, 11,000, and 9,000 in a alpha 2 beta 4 gamma 4 quaternary structure. the three components remained associated during native gel electrophoresis, mono q chromatography, and superose 6 chromatography despite the presence of thiols, glycols, ...19873553184
clinical comparison of piperacillin and cefamandole in treatment of complicated urinary tract infections.the efficacy and safety of piperacillin were compared with those of cefamandole in 72 patients (mean age: 63 years) with complicated urinary tract infections. efficacy was evaluable in 25/34 piperacillin-treated patients (12 gm/day) and in 23/38 cefamandole-treated patients (6 gm/day). clinical cure or improvement was noted in all patients who were given piperacillin and in 96 per cent (22/23) of those who received cefamandole. seventy-one per cent (41/58) of the organisms cultured pretherapy we ...19873554702
comparative, double-blind study of oral ciprofloxacin and intravenous cefotaxime in skin and skin structure infections.the effectiveness and safety of orally administered ciprofloxacin and intravenously administered cefotaxime were compared in a double-blind study of 60 men with infections of skin and soft tissue, including cellulitis, ulcers, abscesses, cellulitis with ulcers or abscesses, wound infections, and post-traumatic infections. patients in the ciprofloxacin group received 750 mg orally every 12 hours for a mean duration of 9.6 days (six to 18 days), and those in the cefotaxime group received 2.0 g int ...19873555040
clinical efficacy of ciprofloxacin therapy for gram-negative bacillary osteomyelitis.the efficacy and toxicity of ciprofloxacin, an orally administered fluoroquinolone, were evaluated in 24 infections in 23 patients with osteomyelitis caused by aerobic gram-negative bacilli. the diagnosis was confirmed by surgical findings and the results of bone biopsy and culture of bone or deep soft tissue. the aerobic gram-negative bacilli were pseudomonas aeruginosa (15 isolates), serratia marcescens (five isolates), escherichia coli (three isolates), enterobacter species (three isolates), ...19873555045
oral ciprofloxacin in the treatment of elderly patients with complicated urinary tract infections due to trimethoprim/sulfamethoxazole-resistant bacteria.the effectiveness and safety of ciprofloxacin, a new quinolone antibiotic, were prospectively evaluated in the treatment of patients with complicated urinary tract infections caused by gram-negative organisms resistant to trimethoprim/sulfamethoxazole. twenty-five elderly (mean age, 70.4 years) patients (24 men and one woman) were enrolled. initial pathogens included pseudomonas aeruginosa (15 isolates), escherichia coli (five isolates), enterobacter aerogenes (one isolate), citrobacter freundii ...19873555051
use of intravenous ciprofloxacin in difficult-to-treat infections.intravenous ciprofloxacin was administered to 54 patients who were either critically ill or in whom oral administration was not possible. the 31 males and 23 females ranged in age from 20 to 89 years (mean, 53.2 +/- 17.8 years). patients had "difficult-to-treat" infections, i.e., respiratory infections (15), abscesses (four intraabdominal, three lung, two soft tissue, and one intrahepatic), deep soft tissue infections (10), chronic post-traumatic osteomyelitis in exacerbation (nine), upper urina ...19873555059
[study of the immune antibody spectrum in blood and plasma donors]. 19873556311
urinary tract pathogens common amongst the inhabitants of a state capital, south west nigeria.in the course of a survey of urine samples collected over a period of 12 months from male and female patients of different socio-economic backgrounds in ibadan, esch. coli, klebsiella spp. pseudomonas aeruginosa, proteus spp., staphylococcus aureus and candida albicans were the most common pathogens isolated. the bacteria were commonly sensitive to the cephalosporins, kanamycin, ampicillin and cotrimoxazole in decreasing order of sensitivity.19863557825
[clinico-bacteriological characteristics of proteus dysbacteriosis in patients with chronic enteritis]. 19873560823
[immunologic prevention of postoperative suppurative-inflammatory complications in cancer of the stomach]. 19863563701
[antibiotic sensitivity of bacteria in the genus proteus in relation to the source of isolation and specific classification].one hundred and ninety-one proteus strains isolated from patients with various pathological processes were studied with respect to their sensitivity to 14 antibiotics. it was shown that their resistance depended on the isolation source and microbial species. amikacin proved to be the most active agent. it was efficient against all the proteus species, irrespective of the isolation source. among the drugs efficient against p. mirabilis and p. vulgaris there were gentamicin, tobramycin and sisomic ...19873566227
worldwide study of cefoperazone susceptibility.in vitro susceptibility to cefoperazone of more than one million clinical aerobic bacterial isolates was evaluated in 369 hospitals in japan, the united states, canada, france, austria, and hungary. standard versions of the disk diffusion method were used according to interpretive criteria approved in the respective countries, and comparisons were made among the countries and among various body sites. susceptibility of organisms varied little within most species among the countries. minor differ ...19873568064
synergy of fosfomycin with beta-lactam antibiotics against staphylococci and aerobic gram-negative bacilli.fosfomycin inhibits bacterial cell wall synthesis at the initial stage. it can act synergistically with beta-lactams. the effect of the combination of fosfomycin and selected penicillins and cephalosporins against staphylococci, pseudomonas aeruginosa, pseudomonas cepacia and selected gram-negative bacteria was determined. synergy was determined by agar dilution and checkerboard titration methods; synergy was defined as an fic index less than or equal to 0.5 and partial synergy greater than 0.5 ...19863569007
trometamol-fosfomycin (monuril) bioavailability and food-drug interaction.a new water-soluble monobasic salt of fosfomycin with trometamol has recently been developed for oral administration. the objective of this study was to evaluate the pharmacokinetics of trometamol-fosfomycin (monuril) in serum and in urine in 10 healthy volunteers after oral administration of one single dose (50 mg/kg). in the same volunteers the concentrations of fosfomycin were measured before and after food absorption, in serum and urine samples taken at t = 0, 2, 4, 6, 8 and 24 h after the d ...19873569381
expanded clinical spectrum of infections caused by proteus penneri.strains of proteus penneri from seven abdominal wounds (after bowel resection), five urine samples, and eight other sites were isolated in mixed cultures. seven urine isolates were in pure cultures. all infections were nosocomially acquired, indicating that complete identification of p. penneri in the clinical laboratory is warranted.19873571463
cytotoxic activity in the coelomic fluid of the annelid eisenia foetida sav.the annelid eisenia foetida not only causes hemolysis of red blood cells of several vertebrate species, but also has a toxic effect on a variety of cell types, such as chicken fibroblasts, guinea-pig polymorphonuclear leukocytes and insect hemocytes. however, it has no influence on the vitality of the coelomocytes of lumbricus terrestris and other lumbricides, nor on the hemocytes of the snail helix pomatia, the mussels anodonta cygnea and unio tumidus, free cells of the turbellarian euplanaria ...19873571568
bacteremia in the spinal cord injury population.positive blood cultures were found in 41 patients on the spinal cord injury service at the milwaukee vamc during the period of july, 1980 to december, 1985 giving an incidence of bacteremia of 1.3%. mortality rate was 17%. most common pathogens were e. coli, proteus mirabilis, serratia marcescens and staphylococcus aureus. a review of 29 available charts revealed genitourinary and respiratory tracts as the most common sources of infection (72.4% and 10.3% respectively). other sources of infectio ...19873572393
post-antibiotic suppressive effect of ciprofloxacin against gram-positive and gram-negative bacteria.the post-antibiotic suppressive effect (pae) of different antibacterial agents against gram-positive bacteria has been known since the 1940s. recently, it has been demonstrated that quinolone antimicrobial agents exert a pae against gram-negative bacteria. in this study, the paes of ciprofloxacin against pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, proteus mirabilis, serratia marcescens, staphylococcus aureus, and streptococcus faecalis were determined. the differences in pae ...19873578330
[effect of loperamide on fecal flora of children with severe prolonged diarrhea].loperamide has recently been proposed in the management of infants with severe protracted diarrhea. the purpose of this study was to determine the effect of loperamide (0.5 mg/kg/d) on fecal flora in 19 cases of severe protracted diarrhea. criteria analysed were: clinical tolerance (vomiting and abdominal distension) and efficacy (number of stools, transit time and na/k in stools); complete identification and counts of aerobic and strict anaerobic bacteria in fresh stools before and 4 to 8 days ...19873579464
the effect of oral non-absorbable antibiotics on the emergence of resistant bacteria in patients in an intensive care unit.critically ill patients admitted to the surgical intensive care unit since 1982 have been treated prophylactically with oral non-absorbable antibiotics combined with parenteral cefotaxime. a mixture of polymyxin e, tobramycin and amphotericin b has been administered via a nasogastric tube and also applied topically to the buccal mucosa. this regimen has proven to be highly effective in reducing the infection rate. the present study evaluated the occurrence of resistant bacteria with this regimen ...19873583971
[postoperative outcome of residual pyelocaliceal stones].the purpose of this paper is to investigate the outcome of postoperative residual stones and their influence to the renal function. we followed up 65 patients (73 kidneys) with residual stones after conventional operation. the follow-up period was on an average 11 years and 9 months. 50 of 73 kidneys were infected before surgery. the most frequently cultured organisms was proteus mirabilis. urinary infection persisted in 34 of 50 kidneys after surgery. the residual stones smaller than 5 mm exist ...19873585041
differences in antimicrobial susceptibilities of clinical isolates in saudi arabia and the united states.in vitro antimicrobial susceptibilities of 3,530 clinical isolates at king faisal specialist hospital and research center (kfsh), in riyadh, saudi arabia, were compared with those of 11,041 bacterial isolates at two hospitals of comparable size in the united states. the gram-negative bacteria, especially escherichia coli, proteus-morganella group, enterobacter sp, serratia marcescens, and pseudomonas aeruginosa were generally more resistant to ampicillin, carbenicillin, cephalosporin, gentamicin ...19873586042
[in vitro chemo-antibiotic sensitivity of gram-negative bacteria from various biological materials. comparison between aztreonam and other agents].527 gram negative bacterial strains isolated from different biological substances were studied by chemo-antibiotic sensitivity. the examination was carried out by the agar diffusion test (kirby-bauer). a very good sensitivity to aztreonam (e. coli, serratia spp., klebsiella spp., proteus spp.), to amikacin (pseudomonas spp., enterobacter spp., citrobacter spp.) and to norfloxacin (citrobacter spp.) was found. even when it was not the best, aztreonam was the second (citrobacter spp.) or the third ...19873587729
[the proteus syndrome]. 19873590910
successful joint arthroplasty following proteus morganii (morganella morganii) septic arthritis: a four-year study.proteus morganii (morganella morganii) is an uncommon cause of septic arthritis. we describe a 53-year-old woman with severely deforming rheumatoid arthritis, who developed an indolent septic arthritis secondary to infection with this organism. she was treated with antibiotics and closed drainage, and subsequently, underwent successful arthroplasty. she continues to do well 4.5 years later. this patient's disease course shows that gram-negative septic arthritis, effectively treated, does not pre ...19873593440
comparative in vitro activity of carumonam (ro 17-2301/ama-1080), a new monobactam, and ceftriaxone against aerobic or facultative gram-negative isolates.carumonam (ro 17-2301/ama-1080) is a new monobactam antibiotic. a comparative in vitro evaluation with ceftriaxone was undertaken against 153 gram-negative clinical aerobic or facultative bacteria, both producers and nonproducers of beta-lactamase. results indicated that carumonam had an enhancement of activity for pseudomonas aeruginosa, klebsiella oxytoca, citrobacter freundii and enterobacter cloacae and parallelled that of ceftriaxone against escherichia coli, klebsiella pneumoniae, proteus ...19873595259
in vitro activity of ro 15-8074 and ro 19-5247 in comparison to cefaclor and cefalexin.805 clinical isolates were investigated for their in vitro sensitivity against ro 15-8074 and ro 19-5247 in comparison to cefaclor and cefalexin in a serial dilution test on solid medium. ro 19-5247 had the strongest activity of all drugs tested against streptococci (except streptococcus faecalis) and was as active as cefaclor and cefalexin against most strains of staphylococcus aureus. ro 19-5247 was the only oral cephalosporin active against bordetella pertussis. it was on average 160 times mo ...19873596808
hemolytic activity and invasiveness in strains of proteus penneri.twenty strains of proteus penneri obtained from the centers for disease control, atlanta, ga., were tested for their ability to hemolyze sheep and human erythrocytes, a property that is thought to be connected with the invasiveness and virulence of proteus species. in the logarithmic phase of growth, p. penneri cultures are hemolytic for such erythrocytes. this ability is comparable to the hemolysis exhibited by nearly 100% of p. vulgaris and p. mirabilis strains, which is due to the production ...19873597752
urinary lithiasis in the black population: an epidemiological study and review of the literature.a retrospective review of 199 black patients with urinary calculi and review of the census figures of the index hospitals revealed that white patients had urinary calculi 3 to 4 times as often as black subjects. the black male-to-female ratio was 1 to 1.55 compared to a ratio for white patients of 2.3 to 1. calculi in black male subjects occur at a younger age than in black or white female or white male patients. the most common organisms cultured in black patients with stones were escherichia c ...19873599198
severe proteus syndrome in an 18-month-old boy.we describe an 18-month-old greek boy with macrocephaly, partial gigantism of hands and feet, hemihypertrophy, pigmented nevi, and other anomalies compatible with the proteus syndrome. cerebral involvement in a most severe form and at an early age concurs with the suggestion that encephalocraniocutaneous lipomatosis may represent a more circumscriptive manifestation of proteus syndrome. localized triggering of several growth factors is a challenging pathogenetic consideration of the syndrome.19873605190
proteus syndrome in southern africa: natural history and clinical manifestations in six individuals.six individuals with proteus syndrome have been investigated; 2 were adults and the others ranged in age from 2 to 11 years. they had a wide spectrum of manifestations and severity together with the hitherto unreported anomalies of penile hypertrophy, macro-orchidism, goiter, and failure of breast development. these findings were associated with normal endocrine function. bizarre digital overgrowth, hemihypertrophy, thickened palms and soles, exostoses of the skull, and multiple hamartomata were ...19873605209
proteus syndrome: an expanded phenotype.we report on 11 new cases of proteus syndrome to illustrate the broad range of the phenotype in this hamartomatous dysplasia. the cardinal manifestations of this sporadic disorder are hemihypertrophy, macrodactyly, exostoses, scoliosis, cavernous hemangiomas, lipomas, linear sebaceous nevi, and deeply rugated soles of the feet. intelligence is usually normal. the differential diagnosis includes klippel-trenaunay-weber and partial lipodystrophy syndromes.19873605210
distribution of cefodizime to exudate in the croton oil-induced rat granuloma pouch and its therapeutic effects on experimental infections in the pouch.cefodizime was compared with cefotaxime (ctx) in regard to its distribution to an inflammatory site (exudate in croton oil-induced granuloma pouch) of rats and its therapeutic effects on experimental infections in such pouches after intravenous injection. cefodizime levels in rat serum and pouch exudate were higher than those of ctx, and the former compound disappeared from the serum and pouch exudate far more slowly than the latter. in the tests for therapeutic effects, cefodizime showed almost ...19873606699
diversity of the phosphoenolpyruvate/glucose phosphotransferase system in the enterobacteriaceae.the presence of the phosphoenolpyruvate glucose phosphotransferase entry routes was studied in 97 genospecies of enterobacteriaceae. phosphoenolpyruvate(pep)-dependent phosphorylation of alpha-methyl-d-glucoside and 2-deoxyglucose was evidenced in 72 species (group i organisms), suggesting the presence of both the iiglc (formerly ii-bglc/iiiglc) and iiman (formerly ii-b/ii-aman) entry routes. erwinia amylovora, budvicia aquatica and all species of leminorella and proteus (as defined by dna relat ...19873606872
the in-vitro activity of cefadroxil, and the interpretation of disc-susceptibility testing.minimum inhibitory concentrations (mics) of cefadroxil were determined for 749 defined clinically-significant bacteria isolated in a london teaching hospital and for 63 strains from an international collection of gram-negative bacilli. assuming a breakpoint of 16 mg/l, for the hospital isolates 81.8% of gram-negative bacilli and 83.4% of gram-positive cocci were sensitive. no significant difference between in-patient, out-patient or community-acquired isolates was found. ninety-five and a half p ...19873610900
[pharmacokinetic and clinical studies of cefuzonam in pediatrics].pharmacokinetic and clinical studies were conducted to evaluate cefuzonam (l-105, czon), a new cephem type antibiotic, in the pediatric field. a total of 9 pediatric patients (2-14 years) was treated with intravenous injection of czon: 4 cases with one shot of 20 mg/kg, 2 cases with one shot of 40 mg/kg and 3 cases with drip infusion over 1 hour of 40 mg/kg. czon concentrations in serum and the excretion in urine were determined. mean serum concentrations of czon after one shot intravenous injec ...19873613084
[clinical evaluation of cefuzonam in pediatrics and a study on the penetration into cerebrospinal fluid].studies were carried out on the penetration of cefuzonam (l-105, czon), a new synthetic cephalosporin antibiotic, into cerebrospinal fluid, and on the clinical efficacy against bacterial infections. the results are summarized as follows: concentrations of czon in cerebrospinal fluid at 1 hour after intravenous administration of 100 mg/kg in cases of furunculosis of the external canal, encephalitis and mumps meningitis were 0.56 micrograms/ml, 1.44 micrograms/ml and 0.33 micrograms/ml, respective ...19873613085
[recent trend of childhood bacterial meningitis in japan (1979-1984). part 4. a classification of prognosis and antibiotic treatment based on causative agents].nine hundred seventy cases of childhood bacterial meningitis treated at 107 institutions in japan from 1979 through 1984 were studied using questionnaire. the number of cases that underwent antimicrobial monotherapy remained nearly constant during the study period, but cases of therapies with beta-lactam combined with aminoglycosides (ags) decreased in number and a gradual increase in the use of beta-lactam combined with non-ags antibiotics including beta-lactam (non ags) was observed. a trend s ...19873613093
[fundamental and clinical studies on cefuzoname in the pediatric field].cefuzoname (czon) one of the aminothiazolyloxyiminoacetamido cephalosporins, was studied for its antibacterial activity, absorption and excretion, concentration in the cerebrospinal fluid (csf) and the penetration, and clinical efficacy. the following are a summary of the results: 1. antibacterial activity; the antibacterial activity of czon was studied on clinically isolated staphylococcus aureus (cefazolin (cez)-susceptible and cez-tolerant strains), escherichia coli, klebsiella pneumoniae, pr ...19873613094
methods of treatment of childhood empyema in a turkish university hospital.in the last ten years, 120 patients were admitted to our clinic with empyema. empyema and effusion have been investigated for pathogenesis. microbiologic studies of pleural aspiration fluid showed that staphylococcus aureus was the most common pathogen, found in 44 patients (36.7 percent). other pathogens found were streptococci in 23 (19.2 percent) and pneumococcus in 18 (15 percent) in sequence. no production occurred in cultures of 19 (15.8 percent) patients. in 16 (13.3 percent), pseudomonas ...19873622031
temocillin in the treatment of pyelonephritis in children.temocillin is a beta-lactamase-stable penicillin with a selective. gram-negative spectrum of activity and a long half-life. previous studies in adult patients have demonstrated its efficacy and safety in the treatment of gram-negative infections. the aim of the study was to evaluate the clinical and bacteriological efficacy and safety of temocillin in children with complicated urinary tract infections. twenty-two children, aged 3 months to 13 years (mean 5.8 years) were treated with temocillin i ...19873622246
comparative analysis of hemolytic and hemagglutinating activities in the coelomic fluid of eisenia foetida and lumbricus terrestris (annelida, lumbricidae).the cf of lumbricus terrestris and eisenia foetida was analyzed with regard to the protein patterns by isoelectric focusing. the cf of e. foetida results in a variety of protein bands; after separation: seven of these with isoelectric points at ph 5.1, 5.2, 5.4, 5.6, 5.8, 6.0 and 6.2 cause the hemolysis of red blood cells. the protein pattern of normal cf of l. terrestris is relatively poor. most of the protein bands are located at the top of the separation (ph less than 5.1) near the anode. how ...19873622885
genetic and biochemical diversity of ureases of proteus, providencia, and morganella species isolated from urinary tract infection.bacterial urease, particularly from proteus mirabilis, has been implicated as a contributing factor in the formation of urinary and kidney stones, obstruction of urinary catheters, and pyelonephritis. weekly urine specimens (n = 1,135) from 32 patients, residing at two chronic-care facilities, with urinary catheters in place for greater than or equal to 30 days yielded 5,088 phenotypically and serotypically diverse bacterial isolates at greater than or equal to 10(5) cfu/ml. a total of 86% of sp ...19873623698
adherence to uroepithelial cells of providencia stuartii isolated from the catheterized urinary tract.the long-term catheterized urinary tract appears to offer a niche for providencia stuartii, otherwise an unusual clinical isolate. p. stuartii, the most frequent and persistent isolate from the urine of 51 long-term catheterized patients, was recovered from 761 of 1230 (62%) weekly urine specimens. to test the hypothesis that prevalence of this species may be due to adherence properties of the organism, 20 selected strains from 14 patients at two nursing homes, representing six distinct serotype ...19863625167
uropathogenicity in rats and mice of providencia stuartii from long-term catheterized patients.providencia stuartii, a frequent and persistent isolate from the urinary tract of chronically catheterized elderly patients, is multiply antibiotic resistant and may cause fatal bacteremia in those patients. we studied p. stuartii strains in rats and mice to determine differences in uropathogenicity. strains studied varied in expression of factors which contribute to pathogenicity of other bacterial species. urinary tract responses following challenge with p. stuartii strain ho (factors expresse ...19873625871
adhesive properties of proteus genus related to antimicrobial agents resistance.the in vitro attachment of 49 proteus spp. to human urinary tract epithelial cells was determined. the antibacterial spectrum to all species of the proteus isolates from hospitalized patients was measured against the most common antibiotics (amikacin, cefamandole, cefoxitin, ceftriaxone, cephalothin, kanamycin, nalidixic acid, oxolinic acid, pipemidic acid, piromidic acid, tobramycin). 18 of them were multiresistant and the other 31 expressed susceptibility to the above antibiotics. bacterial ad ...19873626886
a comparison of cefonicid with other beta-lactams regarding the effect of human and mouse sera on antibacterial activity.the effect of the addition of 50% human or mouse serum on the antibacterial activity of cefonicid, three first generation cephalosporins and ampicillin was studied. human serum added to the test system considerably reduced the activity of cefonicid against staphylococcus aureus and staphylococcus epidermidis strains, and to a lesser degree against proteus mirabilis, escherichia coli and klebsiella pneumoniae strains. human serum also reduced, albeit to a lesser extent, the activities of cephalot ...19873630630
[high mortality of the nile tilapia oreochromis niloticus caused by providencia rettgeri]. 19873632601
in-vitro activity of ticarcillin with and without clavulanic acid against clinical isolates of gram-positive and gram-negative bacteria.the in-vitro activity of ticarcillin with and without clavulanic acid was investigated against 285 freshly isolated clinical strains of gram-positive and gram-negative bacteria by the agar-dilution technique on mueller-hinton-agar. clavulanic acid had an excellent or moderate potentiating effect on the in-vitro activity of ticarcillin against staphylococci, escherichia coli, klebsiella spp., enterobacter aerogenes, proteus mirabilis, citrobacter spp., acinetobacter, haemophilus influenzae and ba ...19863636325
susceptibility of ticarcillin-resistant gram-negative bacilli to different combinations of ticarcillin and clavulanic acid.the susceptibility of ticarcillin-resistant and of ticarcillin-susceptible gram-negative bacilli to different combinations of ticarcillin and clavulanic acid has been investigated. susceptibility to ticarcillin has been restored in ticarcillin-resistant isolates in proportion to the amount of clavulanic acid in the combination and depending upon the species. the decrease in the mics of ticarcillin was excellent to good in proteus, morganella, providencia, escherichia coli and klebsiella, moderat ...19863636338
[urinary tract infections and microbiological characteristics of proteus penneri isolated in taiwan].proteus penneri has been reported to be involved in urinary tract infections and calculi formation. we analyzed 2,265 positive urine cultures from patients hospitalized in taipei veterans general hospital, taiwan and found that 47 patients (2.1%) were infected by p. penneri. most of the patients were male with age over sixty and had been treated with various kinds of antibiotics. most of the patient's urine were collected by catheter. they were alkaline (ph 8.0) and contained amorphous phosphate ...19873652782
gram-negative septicemia caused by providencia stuartii.the role of urologic disease and manipulation of the genito-urinary tract in the pathogenesis of gram-negative bacteremia has been long recognized. among these organisms, species of providencia genus have been increasingly reported. we describe a 17-year-old male paraplegic who developed septic shock due to providencia stuartii following urinary tract manipulation and responding to medical therapy. this is unusual as most cases with septic shock from providencia species will succumb. it is impor ...19873654064
proteus dysbioses in patients with ulcerative colitis.in patients with ulcerative colitis proteus could be isolated from all the gastrointestinal portions studied: mouth cavity, stomach, small intestine, small intestinal mucous membrane, and mostly from faeces. in some patients with ulcerative colitis a decrease in membraneous hydrolysis of polysaccharides was noted. severe proteus dysbiosis was associated with a sharp decrease in absorption processes at the small intestinal mucous membrane. the proteus strains obtained from the patients belonged m ...19873657933
[obstruction of the upper excretory tract associated with primary urinary infection. diagnosis, treatment and course. apropos of 196 cases].the association of upper urinary tract obstruction and urinary tract infection is a relatively common disease which requires early diagnosis and systematic treatment. the diagnosis of this disease can be difficult as many patients present with totally asymptomatic forms which may be revealed suddenly and totally unpredictably by an episode of severe infection. 196 (16%) of the 1,225 patients operated between january 1977 and june 1985 for upper urinary tract obstruction also presented with urina ...19873662445
use of a bioreactor consisting of sequentially aligned l-glutamate dehydrogenase and l-glutamate oxidase for the determination of ammonia by chemiluminescence.a chemiluminometric method for the automated flow injection analysis of ammonia is described. the essence of the invention is the use of a bioreactor consisting of both immobilized l-glutamate dehydrogenase (gldh) and l-glutamate oxidase (glxd), which are sequentially aligned in this order in a minicolumn measuring 2.0 x 20 mm. the unidirectional constant flow of liquid through the column reactor minimizes the reversed diffusion of the solutes so that the following sequence of reactions is ensur ...19873663333
activity in vitro of cgp 31608, a new penem antibacterial agent.the in-vitro activity of cgp 31608 (hereinafter termed cgp), a new penem, was tested by an agar dilution technique in comparison with imipenem, sch 34343, cefotaxime, ceftazidime, aztreonam, ampicillin, gentamicin and ciprofloxacin. 480 clinical isolated were tested, some of which were selected because of their multiple resistance. cgp showed consistent activity against a wide range of species, having mic90 values of 2-8 mg/l for almost all enterobacteriaceae, pseudomonas spp., haemophilus spp., ...19873667480
fractionation of the providencia stuartii cell envelope.cell envelopes (i.e. unfractionated inner and outer membranes) were obtained from providencia stuartii by following procedures previously applied to the isolation of envelopes from escherichia coli. the p. stuartii envelopes contained known inner membrane enzymes that included a variety of dehydrogenases and atpase. the catalytic activity of the atpase depended upon the concentration of magnesium ions, the substrate (atp) level and the ratio of magnesium ions to atp. cell envelopes from p. stuar ...19873668499
[pharmacokinetic, bacteriological and clinical studies of cefuzonam in the field of obstetrics and gynecology].to study the transfer of cefuzonam (czon, l-105) into female genital organs, concentrations of the compound in pelvic dead space exudate were measured in cases of radical hysterectomy due to cervical cancer and analyzed by the two-compartment model. when czon 1 g was drip-infused intravenously, the concentration in the cubital vein blood was 46.95 micrograms/ml at 1 hour after the start of infusion. concentrations in the pelvic dead space exudate reached the peak of 11.29 micrograms/ml at 2.44 h ...19873669285
[urinary tract infections in children. status in a pediatric hospital and sensitivity of bacterial strains to amoxycillin-clavulanic acid combination].three hundred bacterial strains from positive urine cultures were isolated over a 10 months period in paediatric hospitalized and out-patients. in addition to commonly used antibiotics, each strain was tested for amoxycillin-clavulanic acid (augmentin, beecham) susceptibility. this antibiotic, which is a potent inhibitor of bacterial beta lactamases, may be of interest in the treatment of urinary tract infections because of its pharmacokinetics and activity. the antibacterial activity of amoxyci ...19873671031
[urinary tract infections: observations on 19,021 patients hospitalized in sassari province].we report the results of a study carried out upon samples of urine from 19,021 patients in various departments of medical school in sassari and in the hospitals of alghero and ozieri. the 34.7% of samples examined proved to be positive. among these we isolated escherichia coli (32%), proteus spp. (24%), staphylococcus (12%), klebsiella-enterobacter-serratia (kes) spp. (9%), pseudomonas spp. (5%). we found microbic associations in 11% of cases. we also studied the sensitivity of microorganisms to ...19863671668
[hygienic research on meat-dressing shops and enterprises].observations were made on the hygiene state of the meat-conditioning departments and enterprises of 13 meat-dressing combines. both qualitative and quantitative investigations were carried out with regard to 9 bacteriologic indexes. the result of studies on a total of 1,042 samples of washings taken from equipment, working tools, machines, working milieu, and hands and aprons of workers prior to beginning work showed that in case all regulations of cleaning, washing, and disinfection were observ ...19873672919
[electron microscopic-radioautographic study of bacterial invasion in severely burned patients].wound pieces taken from 3-6 sites at a time during surgical treatment of the wound in 10 patients with thermal burns involving 25-50% of the body surface area, were examined by electron-microscopic radioautography and bacteriologically. the burn wounds were found to contain not only live (rna-synthesizing) bacteria but also replicating (dna-synthesizing) bacteria, and to be heterogeneous in terms of bacterial seeding (the largest numbers being present in the scab and the smallest, in the granula ...19873675217
n-nitrosamine formation in urinary-tract infections.urine samples from 31 patients with urinary-tract infections and from 31 controls were analysed for volatile nitrosamines, n-nitrosamino acids, total n-nitroso compounds as a group, and nitrite/nitrate. the concentration of n-nitrosodimethylamine was significantly elevated in urines infected with escherichia coli, proteus mirabilis and klebsiella pneumoniae. the levels of nitrite, n-nitrosoproline and total n-nitroso compounds, when expressed as the amount per mol creatinine, were also significa ...19873679407
[clinical study of astromicin administered by intravenous drip infusion against chronic complicated urinary tract infections].astromicin (astm) was administered by intravenous drip infusion (i.v.d.) to 22 patients with chronic complicated urinary tract infections and the clinical efficacy and safety of this drug were evaluated. the overall clinical efficacy rate obtained was 71.4% (excellent 6; moderate 9) of 21 evaluable cases by the uti committee's criteria. concerning the response on clinical isolates, the drug was highly effective especially against strains of escherichia coli, indole positive proteus and serratia ...19873682183
[fundamental study of piperacillin sodium in term and premature neonates].piperacillin sodium (pipc) is a semisynthetic penicillin displaying high antibacterial activities against gram-positive and gram-negative bacteria including pseudomonas sp., proteus sp., etc. it acts bactericidally and is stable against beta-lactamases. the usefulness of pipc in the treatment of infections in mature and premature neonates was investigated and the following results were obtained. the pharmacokinetics (half-life, distribution volume, total body clearance) of pipc after 50 mg/kg in ...19873682184
radiologic findings in the proteus syndrome.the radiological findings in two patients with the proteus syndrome are described. features in our two cases not previously mentioned or stressed include vertebral dysplasia and enlargement (megaspondylodysplasia), bilateral genu valgum, recurrent after surgery and intraabdominal and mesenteric lipomatosis. emergency laparotomy was performed on the first patient who had a twisted necrotic portion of mesenteric fat. macrodactyly, skeletal muscle atrophy and subcutaneous fat accumulation in the ab ...19873684361
the proteus syndrome: the magnetic resonance and radiological features.the proteus syndrome is a recently delineated group of skeletal and mesodermal malformations. its characteristics include hemihypertrophy and fatty/lymphangiomatous masses. this description outlines the imaging sequences available to the radiologist. it is mainly concerned with the use of magnetic resonance imaging. this shows the extent of the mesodermal malformation with particular reference to the extent of intra abdominal infiltration.19873684362
[statistical studies on bacteria isolated from urinary tract infections (report 4)].the statistics and drug sensitivity tests of bacterial florae isolated from the urinary tract in 1983 and 1984 were reviewed. of the 2,222 strains isolated from outpatients, 593 (26.7%) were gram positive cocci, 21.4% were e. coli, 11.3% were enterococcus, 10.4% were proteus sp., 10.0% were p. aeruginosa, 5.6% were alcaligenes sp., 4.2% were s. epidermidis and the rest were others. of the 507 strains isolated from hospitalized patients, 107 (33.5%) were gram positive cocci, 20.3% were enterococc ...19873687632
infectious nephrolithiasis: results of treatment with methenamine mandelate. 19873691135
[effect of norfloxacin on bacterial flora in human feces].norfloxacin (nflx), a synthetic oral antibacterial agent of quinolone carboxylic acid, was given orally for 5 full days at a dose of 200 mg, three times daily after each meal to healthy normal men with ages between 22 and 25 years weighing 53.0 to 84.0 kg (average 67.0 kg). the actual regimen followed was that the drug was administered twice after lunch and supper on the first day of dosing, and once after breakfast on the last day of dosing. on the 5th day before the start of dosing, on the fir ...19873694785
in vitro activity of a new broad spectrum, beta-lactamase-stable oral cephalosporin, cefixime.cefixime is a new orally absorbed iminomethoxy, aminothiazolyl cephalosporin. it inhibits the majority, 90%, of streptococcus pneumoniae, streptococcus pyogenes, branhamella catarrhalis, haemophilus influenzae, escherichia coli, klebsiella pneumoniae, proteus mirabilis and neisseria gonorrhoeae at concentrations less than or equal to 0.25 micrograms/ml. it inhibits 90% of the other members of the enterobacteriaceae at concentrations less than 1 microgram/ml, with the exception of some strains of ...19873696836
amikacin synergism with beta-lactam antibiotics against selected nosocomial pathogens.the synergistic interaction between amikacin and several investigational antibiotics against seven different genera of nosocomial pathogens was assessed using the microtitre checkerboard technique. the greatest percentage of tests showing synergy was when amikacin was used in combination with apalcillin and azlocillin particularly against serratia marcescens and proteus spp. when amikacin was combined with several new semi-synthetic cephalosporins, synergy was present in a variable percentage of ...19863700292
bacterial adherence to bladder uroepithelial cells in catheter-associated urinary tract infection.to assess the role of bacterial adherence to uroepithelial cells in the pathogenesis of nosocomial urinary tract infection, we prospectively studied 55 patients with indwelling urinary catheters. we obtained uroepithelial cells from the bladder and urine for culture on the patients' entry into the study and every two to four days during catheterization. in all, 235 collections of uroepithelial cells from these patients were used in an in vitro adherence assay with six gram-negative bacterial str ...19863702917
[surgical therapy of intracardiac infected pacemaker electrodes and catheter remnants].with the increase of pacemaker and central venous catheter implantation the number of intracardiac infections now constitutes a significant problem. twenty patients between 22 and 77 years of age admitted for intracardial infected pacemaker probes or central venous catheter remnants presented with recurring attacks of endocarditic fever; in one case multiple pulmonary abscesses resulted from septic embolization. staphylococci were the most frequently involved organism (75%). risk factors leading ...19863705686
ceftriaxone therapy in otolaryngological and pulmonary infections.the efficacy of ceftriaxone, 1 g given intramuscularly once daily, was evaluated in 38 patients with pneumonia (n = 11), pulmonary empyema (n = 2), bronchitis (n = 4), tonsillitis (n = 9), sinusitis (n = 7), and otitis (n = 5). causative organisms were streptococcus pneumoniae (n = 11), viridans type streptococcus (n = 1), haemophilus influenzae (n = 6), group a streptococcus (n = 10), staphylococcus aureus (n = 3), klebsiella pneumoniae (n = 2), pseudomonas aeruginosa (n = 1), escherichia coli ...19863709268
comparison of long-term indwelling catheters and bed-pads in the treatment of urinary incontinence in elderly patients.bed-pads and long-term indwelling catheters were compared in the treatment of urinary incontinence in two groups of eight elderly bedridden women. the same silicone catheter could be left in situ on average for 2 months. a detailed description of the changes of urinary bacterial flora in patients of both experimental groups is given. at the end of the 6 months' study all of the patients in both groups had significant bacteriuria (greater than or equal to 10(8) cfu/1), proteus species being the m ...19863722838
characterization of a haemophilus sp. isolated from a rabbit with conjunctivitis.a case of conjunctivitis in a rabbit caused by a haemophilus sp. is described. this was the only organism that was isolated in large numbers from the infected eye. the uninfected eye of the same rabbit contained staphylococcus epidermidis, corynebacterium kutscheri, proteus mirabilis and a neisseria sp. effects of infection with live haemophilus sp. isolated from the subject rabbit was studied in the eyes of rabbits and mice. swabbing live organisms in the normal rabbit eye produced blepharitis ...19863724056
comparative in vitro antimicrobial activity of chinese medicinal herbs.eighteen herbs used in the treatment of infectious diseases in traditional chinese medicine were evaluated for in vitro activity against ten microbial pathogens. lyophilized teas were tested by the agar dilution technique at 100-1600 micrograms/ml. eleven of the preparations were active against at least one microorganism and six of these were active against at least three of the test isolates. huangqin (scutellaria sp.) and huanglian (coptis sp.) were each active against five of the isolates. hu ...19863724208
[infections of the central nervous system in malignant hemopathies].central nervous system (cns) infections in immunocompromised hosts are often accompanied by subtle disorders because immunosuppression usually decreases the inflammatory response. cns infections in immunocompromised patients are usually caused by organisms different from those found in the general population. the organism causing cns infection in an immunocompromised host can often be predicted if the type of immune abnormality of the patient is known. the common causes of cns infection in immun ...19863726388
antibiotic resistance and conjugative r plasmids in clinical isolates of enterobacteriaceae in peninsular malaysia.twenty-five strains of enterobacteria, isolated from man in peninsular malaysia and consisting of seven enterobacter spp., five escherichia coli, five salmonella spp., four klebsiella spp., two shigella spp., one proteus sp. and and one providencia sp., were tested for antibiotic resistance and conjugative r plasmids. they were all sensitive to nalidixic acid and resistant to at least three antibiotics. the number of resistances ranged from 3 to 11 antibiotics, including cefoperazone and sisomic ...19863726978
in vitro activity of ci-934, a new quinolone, compared with that of other quinolones and other antimicrobial agents.the in vitro activity of ci-934, a new 4-quinolone, was determined against gram-positive and gram-negative bacteria. the mics for 90% of the isolates tested were 0.25 microgram/ml for streptococcus pneumoniae, 0.5 microgram/ml for streptococcus faecalis, 0.25 microgram/ml for staphylococci, including methicillin-resistant strains, and less than or equal to 1.0 microgram/ml for escherichia coli, salmonella and shigella spp., klebsiella spp., proteus spp., and citrobacter spp. ci-934 had activity ...19863729343
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