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in vivo determination of cell mediated immune response in diabetic patients using a multiple intradermal antigen dispenser.the in vivo cell mediated immune response using a multiple intradermal antigen dispenser (multitest) was evaluated in 99 diabetic patients (24 type i and 75 type ii) and in 50 age matched normal subjects. seven different antigens (tetanus, diphteria, streptococcus, tubercoline, candida, trichophyton, proteus and a glycerine control) were applied in the forearm and the induration for the antigens tested was measured 48 hours later. a score was calculated adding the arithmetic means obtained with ...19873500820
urinary pathogens and bacterial sensitivity in hospitalized urological patients based upon clinical aspects.for a total of 396 hospitalized urological patients with complicated and/or hospital-acquired urinary tract infections (uti) urinary pathogens with colony counts of 10(5)/ml or more were determined, several species were then subclassified by epidemiological markers. the minimal inhibitory concentrations (mic) were measured using the agar dilution method for seven penicillins and for four penicillin combinations, for six oral and 14 parenteral cefalosporins, for three older and five newer quinolo ...19873501901
gram-negative septicemia in american alligators (alligator mississippiensis).six species of bacteria (family enterobacteriaceae) not commonly reported as associated with disease in american alligators (alligator mississippiensis) were documented, suggesting that aeromonas is not the only bacterium responsible for septicemia in crocodilians. these included citrobacter freundii, enterobacter agglomerans, proteus sp., morganella morganii, serratia marcescens, and klebsiella oxytoca. clinical signs of disease included intensive basking, anorexia, lethargy, flaccid limb paral ...19863503133
renal function and fractional clearances of american river otters (lutra canadensis).the finely lobulated kidneys of american river otters (lutra canadensis) are not visualized on plain abdominal radiographs. similar values for blood urea nitrogen (bun), creatinine, and uric acid were obtained on different analytical systems used in 1984 and 1985. the mean +/- sd for measured plasma osmolalities (309.80 +/- 8.86 mosmol/kg) of otters in 1985 was significantly (p less than 0.01) less than that of calculated serum osmolalities in the same 1985 specimens (321.61 +/- 5.64 mosmol/kg) ...19863503142
[antibiotic resistance at a hospital environment. observations from 1980 to 1986].a total of 1659 bacteria strains were examined for susceptibility to different drugs by the kirby-bauer method. the isolates came from the general medicine ward of castellamonte's hospital (ussl 40). the results were retrospectively studied and statistically elaborated. the study lasted from 2 to 7 years depending on the antibiotic considered. the isolates included: escherichia coli, klebsiella spp., serratia spp., citrobacter spp., proteus mirabilis, proteus ind+, pseudomonas aeruginosa, pseudo ...19873509032
lectins for the identification of ocular bacterial pathogens.in a preliminary in vitro investigation, fluorescein-conjugated lectins were used in the identification of bacteria commonly involved in ocular infections. clinical isolates of staphylococcus aureus, staph. epidermidis, streptococcus pneumoniae, strep. pyogenes, pseudomonas aeruginosa, hemophilus influenzae, and proteus mirabilis were incubated with each of eleven lectins using a slide technique. bacterial fluorescence was readily observed with a fluorescence microscope. all clinical isolates bo ...19873509280
the effects of chronic cystitis on vesicoureteral reflux in an animal model.the purpose of this study was to determine if chronic cystitis interferes with maturation of the ureterovesical junction, thus prolonging vesicoureteral reflux in infant rhesus monkeys (macaca mulatta). serial implantations of a proteus-infested silastic pellet into the bladder wall ensured continued infection as demonstrated by urine culture. chronic cystitis as evidenced by pathological findings at surgery and sacrifice was created in a group of infant monkeys who had initial spontaneous vesic ...19863510317
charles proteus steinmetz: pioneer in research on electric motors. 19863510340
intestinal bacteria translocate into experimental intra-abdominal abscesses.experimental intra-abdominal abscesses were initiated by surgical implantation of a fibrin clot contaminated with either bacteroides fragilis, bacteroides thetaiotaomicron, or b fragilis-escherichia coli. seven days after surgery the numbers of bacteroides (per gram) in b fragilis and b thetaiotaomicron abscesses were typically log10 8.4 +/- 0.5 (n = 6) and log10 6.4 +/- 0.6 (n = 4), respectively; b fragilis-e coli abscesses typically contained log10 8.9 +/- 0.5 b fragilis and log10 7.6 +/- 0.6 ...19863510604
neutrophil activity in abscess-bearing mice: comparative studies with neutrophils isolated from peripheral blood, elicited peritoneal exudates, and abscesses.intraabdominal abscesses were induced in mice by intraperitoneal inoculation of bacteroides fragilis and escherichia coli plus bran as the abscess-potentiating agent. six- or seven-day-old abscesses were mechanically disaggregated in buffer, and the cells obtained were fractionated on discontinuous percoll density gradients. neutrophil populations of different density, each approximately 90% pure, were isolated. when the abscess-derived neutrophils were subsequently incubated with normal serum i ...19863512441
rare occurrence of proteus vulgaris in faeces: a reason for its rare association with urinary tract infections.the faecal carriage rates of different species of proteeae were assessed in studies with 220 faecal isolates from 219 individuals of whom approximately one-third were well and the remainder had gastro-enteritis. as a result of the development of new media that allowed replacement of the phenylalanine deaminase test with the tryptophan deaminase test and made it possible to combine tests for indole and urease production and for hydrogen sulphide and ornithine decarboxylase formation in two single ...19863512839
septic thrombosis of the dural venous sinuses.from 1940 to 1984, 19 cases of septic dural-sinus thrombosis have been diagnosed at the massachusetts general hospital, and some 136 cases have been reported from other institutions. septic thrombosis most frequently involves the cavernous sinuses (96 cases). facial or sphenoid air sinus infection often precede cavernous-sinus disease. in addition to the classical signs of proptosis, chemosis, and oculomotor paralysis, isolated sixth-nerve palsy and hypo- or hyperesthesia of the fifth nerve may ...19863512953
endophthalmitis following intraocular lens implantation: report of 30 cases and review of the literature.bacterial endophthalmitis is a postoperative complication of intraocular lens implantation. a review of 30 cases showed that 77% occurred within seven days of initial cataract surgery and that all cases occurred within 32 days. common presenting symptoms and signs included pain localized to the involved eye, decreased visual acuity, conjunctival injection, anterior chamber inflammation, hypopyon, and absent or poor red reflex. diagnosis of an infectious etiology was made by aqueous and vitreous ...19863513284
direct antimicrobial susceptibility testing of urines positive in the ms-2 urine screening program.a study was performed using the abbott ms-2 system, in which positive urine screening specimens were set up directly to an antimicrobial susceptibility test. these results were compared with standard techniques. the overall correlation for urines containing single pathogens was 95.4%. results were available 5-8 hr after receipt by the laboratory.19863514104
use of ceftazidime in febrile incidents in immunocompromised haematological patients.ceftazidime was used in twenty-eight febrile episodes recorded in twenty-two neutropenic patients (eleven of each sex, aged between 15 and 73 years) who were immunologically compromised (nineteen acute leukaemias and three malignant lymphomas treated with cytostatics). seven pathogens were isolated in six patients (four klebsiella, one proteus, one escherichia coli and one enterobacter). the clinical response was cure in ten cases, improvement in six, failure in four and unassessable in eight. i ...19863514313
role of cell surface receptors in the regulation of intracellular killing of bacteria by murine peritoneal exudate neutrophils.the role of the fc and third component of complement (c3) receptors on mouse neutrophils in the control of killing of proteus mirabilis, opsonized in normal mouse serum (nms) or heated immune mouse serum (hims), was studied. the events following incubation of neutrophils with p. mirabilis and the events associated with bacterial killing were assayed. the respiratory burst was quantified by chemiluminescence (cl). levels of leukocyte-associated bacteria were determined after a 20-min ingestion pe ...19863514456
comparative activity of ofloxacin with reference to bacterial strains isolated in in-patients and out-patients.the in vitro activity of ofloxacin, a new quinolone derivative, was compared to that of other agents commonly in use against pathogens isolated in the community and in the hospital. none of the community or hospital strains isolated from urinary tract infection showed resistance to ofloxacin, while variable resistance was demonstrable with all other oral agents. similar results were obtained with pathogens isolated from infected wounds originating in the community and in the hospital. among path ...19863514469
the antimicrobial activities of trimethoprim and sulfonamides.the folate inhibitor trimethoprim (tmp) is active against and potentially cidal to a few higher microorganisms and a wide spectrum of pathogenic bacteria except for bacteroides, branhamella, brucella, chlamydia, clostridium, mycobacterium, mycoplasma, nocardia, neisseria, pseudomonas and treponema. these organisms tend to be more sensitive to sulfonamides (sul) than to tmp, whereas tmp is 10- to 100-fold more active than sul against most other bacteria. synergy between tmp and sul occurs at drug ...19863515508
pipemidic acid, a new treatment for recurrent urinary tract infection in small animals.a new chemotherapeutic agent, pipemidic acid, was used to treat 14 dogs and 2 cats with recurrent urinary tract infection caused by multiresistent strains of escherichia coli and proteus spp. bacterial culture of the urine after treatment revealed disappearance of the microorganisms in all patients. it is concluded that pipemidic acid is a promising chemotherapeutic agent for urinary tract infections caused by multiresistant e. coli and proteus spp., with the condition that bacterial culture dur ...19863515744
occurrence and pathogenic role of morganella-proteus-providencia group bacteria in human feces.a total of 2,693 fecal specimens, with 1,422 from healthy persons and 1,271 from patients suffering from enteric diseases, was investigated to isolate species of the morganella-proteus-providencia group and to evaluate the role of these bacteria in intestinal disorders. most strains were isolated from two media, i.e., blood agar and tryptophan agar. two of the species were more frequently found in diarrheal cases than in healthy controls. these species were morganella morganii and proteus mirabi ...19863517057
in vitro and in vivo study of stone formation by corynebacterium group d2 (corynebacterium urealyticum).corynebacterium group d2 inoculated into normal human urine formed struvite crystals and an increase in ph and ammonium concentration after 24 h of incubation. zinc disks dipped into a broth culture of this microorganism and inserted into the bladders of rats produced stones with a mean weight of 12.5 mg (ranging from 1 to 57.7 mg) after 12 days. analysis of the infrared spectrum determined the stones to be composed of struvite. from these results its seems that stone formation by corynebacteriu ...19863517059
effect of inoculum size on ampicillin and amoxycillin susceptibility determined by gas-liquid chromatography for members of the family enterobacteriaceae.inoculum size had only a small effect on the results for ampicillin and amoxycillin susceptibility testing of escherichia coli. in contrast, a difference in inoculum size from 10(6) to 10(8) cfu/ml profoundly affected the results for ampicillin and amoxycillin susceptibility testing of proteus mirabilis, causing a change from susceptibility to complete resistance. these different effects of inoculum size were observed both when susceptibility was determined by the suppression of a characteristic ...19863517063
self-disinfecting plastics for intravenous catheters and prosthetic inserts.a disinfectant (2,4,4'-trichloro-2'-hydroxydiphenyl ether: irgasan, ciba-geigy) was incorporated into plastic washers fabricated from ethylvinyl acetate (eva), polyethylene, polypropylene or tpx. plastics containing 0.2 and 2% irgasan gave zones of inhibition on nutrient and blood agar plates seeded with micro-organisms (staphylococcus aureus, staph. epidermidis, escherichia coli, proteus mirabilis or candida albicans) even after thorough washing. exceptionally, c. albicans was inhibited only by ...19863517154
isolation and identification of two antibacterial agents produced by a strain of proteus mirabilis isolated from larvae of the screwworm (cochliomyia hominivorax) (diptera: calliphoridae). 19863517334
[effect of urinary tract infections on the effectiveness of the litholytic action of debelysin]. 19863517831
[elimination of klebsiella pneumoniae and humoral immunity in immunization with the is-4 immunostimulating mixture].the present investigation has revealed that in mice, immunized with preparation hc-4 (an immunostimulating agent consisting of water-soluble antigenic complexes obtained from 4 opportunistic microorganisms: klebsiella pneumoniae, staphylococcus, proteus and escherichia coli k-100 having a common antigen with haemophilus influenzae) and challenged with k. pneumoniae culture on day 7 after immunization, the complete elimination of k. pneumoniae from the blood occurs within 24 hours. the subcutaneo ...19863518307
[effect of human immunoglobulins on microflora of the large intestine in nonspecific ulcerative colitis].the use of commercial preparations of human immunoglobulin for the treatment of ulcerous colitis produces a positive effect on the microflora of the large intestine, contributing to the disappearance of proteus, the lactose-negative forms of enterobacteria and the hemolytic variants of staphylococci, as well as to the increase of the amount of useful indigenous microflora (bifidobacteria and lactobacteria). the quantitative and qualitative improvement of the microflora leads, possibly, to the de ...19863518310
opportunistic bacteria isolated from the caecum of rabbits with mucoid enteritis. 19863518857
inhibitory and bactericidal activity of cefmetazole and seven other beta-lactams against different bacterial inocula.the determination of the minimal inhibitory concentrations (mics) at different bacterial inocula represents an important factor in determining the potential effectiveness of new antibiotics. mics at different inocula (10(5) and 10(7) cfu/ml) and minimal bactericidal concentrations (mbc) of cefmetazole and seven other new cephalosporins (ceftazidime, ceftizoxime, cefamandole, ceftriaxone, cefotaxime, cefuroxime, cefoxitin) were determined by a miniaturized dilution broth method against a wide ran ...19863518963
norfloxacin versus pipemidic acid in complicated urinary tract infections due to susceptible pathogens: a comparative clinical trial.norfloxacin (nor) was compared to pipemidic acid (pa) in complicated urinary tract infections (utis) caused by pathogens susceptible to both agents to evaluate the antibacterial activity of the new 4-quinolone derivative. sixty-five patients were randomly allocated to receive nor or pa, 400 mg bid for 7 days, and the results evaluated for treatment efficacy at 5 weeks after completion of therapy. overall microbiological cure was significantly greater in nor-treated patients, as 19 of 26 were cur ...19863518966
allogeneic transfusion of macrophages in acute urinary tract infection.three patients with acute urinary tract infections that failed to respond to antibiotics were treated by allogeneic peritoneal macrophage transfusions. no harmful effects were observed following macrophage transfusions and the urinary infections were eradicated in treated patients. it could be concluded that allogeneic macrophage transfusion is feasible between unrelated individuals and can combat severe resistant urinary infections.19863519021
inducible beta-lactamases are principally responsible for the naturally occurring resistance towards beta-lactam antibiotics in proteus vulgaris.the role of inducible chromosomally mediated beta-lactamases was studied in 22 proteus vulgaris isolates by monitoring enzyme induction in the presence of various inducers such as ampicillin, cefalothin, cefuroxime, cefsulodin, 6-aminopenicillanic acid (6-aps), and imipenem. 20 of the isolates exhibited resistance to ampicillin, cefalothin, and cefuroxime, whereas 2 isolates were susceptible to these compounds. in all resistant isolates marked inoculum effects could be observed. enzyme productio ...19863519112
cephalosporins in cutaneous infections. a prospective comparison of two dosage regimens of ceftazidime for therapy of skin and skin structure infections.the safety and efficacy of ceftazidime administered as 0.5 g every 8 hours (q8h) or 1.0 g q8h for at least 5 days were compared in 197 patients and found to be effective in the treatment of cellulitis, abscesses, skin ulcers, and wound infections. staphylococcus aureus was the predominant pathogen in both treatment groups with approximately half of the isolates from each treatment group being gram-positive. pseudomonas aeruginosa was the most common gram-negative isolate. p. aeruginosa, proteus ...19863519482
review of imipenem.imipenem, a new carbapenem beta-lactam broad-spectrum antibiotic, is highly active in vitro against most aerobic and anaerobic gram-positive and gram-negative bacteria isolated from infectious diseases of human beings. except for enterococci and methicillin-resistant staphylococci most gram-positive cocci are inhibited by less than 2 micrograms/ml. although the mic-90 of methicillin-resistant staphylococci may be less than 4 micrograms/ml, these bacteria are usually resistant to imipenem by modi ...19863519499
induction of beta-lactamase in proteus vulgaris.various beta-lactam antibiotics, including monocyclic beta-lactams, induced the beta-lactamase of proteus vulgaris; when clinical isolates were induced by benzylpenicillin, each strain produced a single beta-lactamase but the activity per milligram dry weight differed from strain to strain. the beta-lactamases of the p. vulgaris strains were heterogeneous with respect to their isoelectric points, but had almost the same specific activities, substrate specificities and michaelis constants. the ki ...19863519851
crystallization of reca protein from proteus mirabilis.the reca protein from proteus mirabilis, which is homologous to the escherichia coli protein, forms crystals in the orthorhombic space group p2(1)2(1)2(1). there are two 38,000 molecular weight subunits in the asymmetric unit and the unit cell dimensions are a = 57.5, b = 127.0 and c = 157.0 a.19863519980
[putrescent and septic wound complications after appendectomy]. 19863520103
[comparative clinico-laboratory studies of acute intestinal diseases of klebsiella and klebsiella and proteus etiology in young children]. 19863520472
[the mutagenicity of cordemcura].the mutagenic potential of cordemcura was investigated. cordemcura showed no mutagenic response in the tests, either in the presence or in the absence of an activation system.19863520604
in vivo evaluation of a-56619 (difloxacin) and a-56620: new aryl-fluoroquinolones.a-56619 and a-56620 are two new aryl-fluoroquinolones which are as potent as or more potent than norfloxacin when administered orally and subcutaneously in mouse protection tests against staphylococcus aureus, streptococcus pyogenes, and streptococcus pneumoniae. a-56619 and a-56620 were more potent than norfloxacin when administered orally against escherichia coli, proteus mirabilis, serratia marcescens, and pseudomonas aeruginosa. a-56620 was as potent or two- to threefold more potent than nor ...19863521473
reduction of cephamycin concentrations at the infection site in mice with experimental peritoneal infection caused by cephalosporinase-producing bacteria.in an experimental model of peritoneal infection by cephalosporinase- (ia and ic) producing bacteria in mice, the reduction of cefoxitin, cefmetazole, and cefazolin concentrations in peritoneal fluid was observed in the mice infected with the ia enzyme producer, whereas cefbuperazone concentrations were not reduced.19863521482
isolation, characterization, and mode of action on escherichia coli strains of microcin d93.microcin d93 is an antibiotic substance produced by escherichia coli strains which harbor the 5.5-kilobase plasmid pmccd93. its production is unaffected by the use of different carbon and ammonia sources, different phosphate concentrations, or mitomycin c. we developed a method for purifying this microcin based on gel permeation chromatography and reverse-phase high-pressure liquid chromatography. the antibiotic appears to be a small, hydrophilic, basic peptide, active on e. coli and proteus, ci ...19863521486
[neonatal epidemic diarrhea caused by proteus mirabilis]. 19863521888
correlation between structure and biological properties of cephalosporins: the discovery of ceftriaxone.a research programme on cephalosporins was conducted in the author's laboratory with the aim of creating compounds with improved antibacterial and pharmacokinetic properties. in the first phase of this programme, great attention was paid to the study of how the structure of a 3-heterocyclic-thiomethyl side chain is capable of influencing antibiotic activity within a large series of model compounds possessing the same acyl side chain (2-thienylacetyl) as cephalothin. several structural and physic ...19863522153
nosocomial infection in a children's hospital. a retrospective study.the investigations the frequency of the occurrences of hospital infections were carried in a general pediatric ward. most of the infections (77 per cent) appeared in children up to one year of age. the hospital infections mostly affected children residing in rural areas. the etiological agent was e. coli (36 per cent), proteus (22.7 per cent) klebsiella and staphylococcus. the infections caused by e. coli mainly appeared in girls, others- in boys. the frequency of the occurrences of hospital inf ...19863522729
bacterial antagonists of shigellae.bacterial antagonism between a microorganisms and shigella sonnei strains was studied in model experiments simulating conditions of the natural aquatic environment. in these studies surface waste samples from the river vltava served as the experimental environment. to ensure bacteriologically defined conditions all water samples were heat-sterilized prior to antagonism testing. consistently with the literature data and author's own observations the following bacterial species and genera were cho ...19863522730
[norfloxacin in the treatment of urinary tract infections].28 patients with urinary tract infections (uti) were treated with norfloxacin 2 x 400 mg daily for 9 days. 14 of these patients had complicated uti (stones, obstruction). the main pathogens were e. coli and proteus mirabilis. on the third day of treatment all bacteria were eradicated. one week after therapy all patients with uncomplicated uti were cured and, likewise, 9 of 14 with complicated uti. there was no laboratory evidence of adverse effects. 6 patients reported only mild side effects suc ...19863524016
[comparative evaluation of different methods for typing bacteria in the genus proteus].the work presents the comparative evaluation of different methods used for differentiating bacteria of the genus proteus. a scheme of the combined typing of proteus is presented. this scheme includes the determination of enzymatic and serological variants with the subsequent additional bacteriocinogenic and phage typing. among the proteus strains in our collection (600 strains), 123 sero-enzymatic variants, 71 enzymo-lysogenic variants, 64 enzymo-bacteriocinogenic variants, 162 sero-lysogenic va ...19863524078
relationships between human blood groups, bacterial pathogens, and urinary tract infections.blood groups of 137 patients with acute pyelonephritis and chronic upper tract infection, cystitis, and asymptomatic bacteriuria were compared with those of a normal uninfected control population. in addition, the identified uropathogens were categorized according to the patient's blood group. there was a significant association between the diagnosis of chronic upper tract infection and blood group b as compared with controls (p = less than 0.05, chi 2). analysis of the bacterial isolates showed ...19863524229
urinary pathogenicity of proteus mirabilis strains isolated from faeces or urine.urinary and faecal isolates of proteus mirabilis were studied with respect to a number of bacterial properties as possible virulence factors in the pathogenesis of urinary tract infections: experimental virulence in a mouse model, haemolysin production, haemagglutinating properties, hydrophobicity of the bacterial surface, sensitivity to the bactericidal effect of human serum, serotype and cell invasiveness. urinary isolates were slightly more virulent than faecal isolates in the mouse model. no ...19863524444
the functional localization of cytochromes b in the respiratory chain of anaerobically grown proteus mirabilis.the functional localization of the cytochromes b found in anaerobically grown proteus mirabilis was investigated. from light absorption spectra, scanned during uninhibited and hqno-inhibited electron transport to various electron acceptors, it was concluded that all cytochromes b function between two hqno inhibition sites, or more probably in a q- or b-cycle.19863524446
lipopolysaccharide of providencia rettgeri. chemical studies and taxonomical implications.the chemical constitutional analysis of the lipopolysaccharide (lps) isolated from providencia rettgeri was carried out. polyacrylamide gel electrophoresis using sodium dodecylsulfate or sodium deoxycholate showed that the lipopolysaccharide mostly consisted of short sugar chains. the lipid a was precipitated out after mild acid hydrolysis of lps. from the supernatant degraded polysaccharide and unsubstituted core fractions were isolated. compositional analysis of the core material revealed the ...19863524498
in-vitro antibacterial activity of bo-1165, a new monobactam antibiotic.bo-1165 has excellent antibacterial activity against gram-negative bacteria but it is almost inactive against gram-positive and anaerobic bacteria. bo-1165 was more active than the four reference drugs against escherichia coli, salmonella spp., shigella spp., klebsiella pneumoniae, serratia marcescens, indole-positive and indole-negative proteus. also, bo-1165 exhibited high antibacterial activity against strains of pseudomonas aeruginosa (mic50, 3.12 mg/l) and p. cepacia (mic50, 1.56 mg/l), but ...19863525497
proteus penneri showing a green colour reaction with kovacs' indole reagent.all 33 investigated strains of proteus penneri gave a green colour reaction with kovacs' indole reagent after an incubation of about three days at 36 degrees c. furthermore, 26% of the 51 strains of proteus mirabilis studied also showed the green colour reaction but somewhat more weakly. the initial compound of this reaction seems to be a product of tryptophan metabolism which is formed only under aerobic conditions.19863526760
[a case of soft vesical calculi].a case of soft vesical calculi is reported. a 72-year-old male, visited our clinic complaining of discharge of white muddy substance. kub film revealed calcification in the vesical region. urinalysis showed uti, and culture of urine was positive for proteus mirabilis. thereafter, the patient suffered from discharges of similar calculi several times. two of three infrared spectroscopic charts showed mixture of calcium phosphate and ammonium hydrogen urate and that of the remaining disclosed magne ...19863526838
[effect of cationic polymers and surface-active substances on bacterial resistance to kanamycin].the effect of cationic polymers and surface active substances (sas) on sensitivity of klebsiella aerogenes 600, escherichia coli 154 and proteus vulgaris 7470 to kanamycin was studied. a decrease in the resistance of the above organisms to kanamycin on its use in combination with cationic polymers and sas was observed. it was shown that such substances inhibited the activity of the enzymes inactivating kanamycin. their effect was suggested to be due to changes in the lipid surrounding of the enz ...19863527060
significance of non-pathogenic cross reactive bowel flora in patients with ankylosing spondylitis.we have previously shown that antisera raised in rabbits to certain enteric cross reactive strains of bacteria are capable of specifically lysing the peripheral blood lymphocytes of hla-b27 positive patients with ankylosing spondylitis (b27+ as+). we now report that bacteria with cross reactive antigenic determinants are found in the bowel flora of all of 52 b27+ as+ patients but in only one of 50 hla-b27 positive normal controls (b27+ as-). these organisms are functionally similar to the cross ...19863527085
high-level trimethoprim resistance in urinary bacteria.the results of a three year evaluation of the incidence and type of trimethoprim resistance in pathogens responsible for significant bacteriuria in a general hospital in edinburgh uk, are presented and compared to results of a previous study. in the present study, trimethoprim resistance was 50% more frequent in bacteria isolated from men and nearly twice as frequent in bacteria from elderly patients. however, the proportion of trimethoprim resistant strains fell annually when resistance was mea ...19863527699
late intraphagosomal hydrogen ion concentration favors the in vitro antimicrobial capacity of a 37-kilodalton cationic granule protein of human neutrophil granulocytes.we described previously (w.m. shafer, l.e. martin, and j.k. spitznagel, infect. immun. 45:29-35, 1984) the presence of a 37-kilodalton cationic antimicrobial protein (37k cap) in extracts of granules prepared from human polymorphonuclear granulocytes (pmn). in this investigation, we prepared 37k cap from pmn granule extracts by sequential ion-exchange and molecular-sieve chromatography and examined its antimicrobial activity against a number of gram-negative and gram-positive bacteria. at concen ...19863527987
external quality assessment for clinical microbiology in norway 1985.during 1985 four external quality assessment tests for clinical microbiology were performed, each consisting of four simulated clinical specimens. the results are reported, evaluated and some problem areas discussed.19863528927
severe renal sepsis in the very elderly. 19863529066
[current resistance in surgical infections and its development since 1970].more than 7,000 bacteriological samples were received by the authors and were analysed for the frequency of resistance among pyogenic cocci which are relevant to surgical practice. staphylococcus aureus accounted for 41 per cent and escherichia coli for 15.3 per cent. these were the most common germs in 1984. resistance of staphylococcus aureus to ampicillin was as high as 78 per cent. resistance levels below eight per cent were recorded in the context of lincomycin, erythromycin, gentamicin, ox ...19863529749
ferric ammonium citrate decomposition--a taxonomic tool for gram-negative bacteria.the iron uptake test of szabó and vandra has been modified and used for the differentiation of gram-negative bacteria. nutrient agar containing 20 g per litre of ferric ammonium citrate was distributed into narrow tubes and solidified so as to form butts and slants. considering the localization of the rusty-brown coloration produced after seeding and incubation, 2367 strains were classified into four groups. (1) unchanged medium: escherichia coli, shigella spp., yersinia spp., hafnia alvei and m ...19863529797
therapeutic effect of sf-2103a, a novel carbapenem antibiotic, in combination with cefotaxime, cefoperazone and other cephalosporins.combinations of sf-2103a with cefotaxime, cefoperazone or cefazolin showed synergistic efficacy at a wide range of combination ratios against experimental infection in mice due to proteus vulgaris gn76/c-1, producing type ic cephalosporinase, escherichia coli no. 29/36 rgn823, producing type iiia (tem-2) penicillinase and e. coli gn206, producing type ib cephalosporinase. these effects by sf-2103a were greater than those seen with sulbactam. the in vitro and in vivo synergistic activities were r ...19863531130
expression and regulation of the penicillin g acylase gene from proteus rettgeri cloned in escherichia coli.the penicillin g acylase genes from the proteus rettgeri wild type and from a hyperproducing mutant which is resistant to succinate repression were cloned in escherichia coli k-12. expression of both wild-type and mutant p. rettgeri acylase genes in e. coli k-12 was independent of orientation in the cloning vehicle and apparently resulted from recognition in e. coli of the p. rettgeri promoter sequences. the p. rettgeri acylase was secreted into the e. coli periplasmic space and was composed of ...19863531181
trimethoprim-sulfamethoxazole therapy of neonatal proteus mirabilis meningitis unresponsive to cefotaxime. 19863532051
[a case of septic necrobiosis in leiomyoma of the uterus in the postpartum period. clinical, echographic and x-ray computed tomography study].the authors report a case of septic necrobiosis of leiomyoma uteri the first signs of which were seen at the eighth day after childbirth. after several episodes of discharge of proteus into the blood, separated by a period of apparent resolution, the disorder was only resolved by hysterectomy carried out upon the request of intensive care staff, despite the desire of the patient for subsequent pregnancies. bacteriological examination of the necrobiotic myoma showed the presence of proteus morgan ...19863532279
[acute obstructive pyelonephritis (experimental research)]. 19863532486
[effect of prodigiozan, levamisole and methyluracil on the course of an experimental infection and primary immune response].the effect of prodigiosan, levamisol and methyluracil on the process of experimental sepsis caused by proteus was studied comparatively on mice, and the primary immune response was studied on intact animals treated with prednisolone, cyclophosphan or azathioprin. it was shown that prodigiosan was a more active stimulator of antiinfectious resistance of the intact animals than levamisol or methyluracil. its effect was evident with administration before the infection, while the effect of levamisol ...19863532939
[prevalence and care of patients with proteus mirabilis enteritis]. 19863533289
proteus mirabilis: taxonomic position, peculiarities of growth, components of the cell envelope. 19863533451
[a simple enzyme assay for the simultaneous determination of penicillin derivatives and clavulanic acid in biological fluids].a simple enzymatic assay for simultaneous determinations of amino-/ureidopenicillins and clavulanic acid concentrations in various body fluids is described; no pretreatment of the samples is required. the assay is based on the competitive inhibition of the chromogenic compound padac by clavulanic acid in the presence of proteus vulgaris beta-lactamase. detection limit for clavulanic acid amounts 0.02 mg/l. interferences with other antibiotics such as aminoglycosides, macrolides, quinolones etc. ...19863533762
effects of bacteroides asaccharolyticus cells and b. fragilis surface components on serum opsonisation and phagocytosis.the effect of bacteroides asaccharolyticus on the opsonisation and phagocytosis of gram-positive and gram-negative bacteria by human polymorphonuclear leukocytes (pmnl) was investigated. uptake of most isolates of staphylococci, streptococci and clostridia by pmnl after opsonisation with serum treated with b. asaccharolyticus was largely unimpaired. the same treatment of serum before opsonisation of isolates of pseudomonas, enterobacter, klebsiella and gardnerella resulted in the uptake by pmnl ...19863534273
[clinical evaluation of timentin in intensive care].16 patients admitted to a micu were treated with timentin (ticarcillin + clavulanic acid) for bacterial infections: 11 cases of pulmonary infection, 3 cases of urinary tract infection, 2 septic shocks, 3 septicemias and 1 case of multifocal infection. the pathogens considered as a firmly established cause of infection were: 5 acinetobacter, 12 pseudomonas, 3 serratia, 4 klebsiella, 1 e. coli, 2 proteus, 1 providencia, 1 staphylococcus aureus, 1 staphylococcus epidermidis, 1 streptococcus d and 1 ...19863534719
[urinary tract infections in general medical practice. a study of clinical findings, of the distribution of causative microbial species and their sensitivity to 16 antibiotics].from april to july 1984, all of the strains recovered by 10 private laboratories in upper-normandy region (france) from urine samples from outpatients seen in office practices (public and private hospitals excluded) were preserved. a total of 829 strains were collected, identified and tested against antibiotics using disk diffusion techniques. these investigations, together with a synthesis of clinical findings, were carried out by the laboratory of bacteriology of the rouen university hospital. ...19863534727
[clinical and bacteriological study of o-demethylfortimicin a sulfate in urinary infection].o-demethyl-fortimicin-a-sulfate (odmf) is a semisynthetic derivative of fortimicin-a with increased in vitro activity especially against pseudomonas aeruginosa. this study was designed to determine the efficacy of odmf in the treatment of uti and to evaluate the in vitro susceptibility of bacteria isolated from the urine of patients to odmf as compared to tobramycin (to), gentamicin (ge) and amikacin (ak). in 28 hospitalized patients with acute uti, odmf (free base) in a dose of 2 to 5 mg/kg was ...19863534728
[ofloxacin (ru 43280): clinical evaluation in urinary and prostatic infections].ofloxacin, a new fluoroquinolone, was given to fifty patients (29 females and 21 males) aged 25 to 86 years with urinary tract infection or prostatitis. urinary tract infections usually chronic and associated with urologic anomalies, included 17 cases of cystitis and 19 cases of pyelonephritis. 14 patients had prostatitis. pathogens recovered from the urine were 26 e. coli, 2 citrobacter, 4 proteus mirabilis, 2 klebsiella, 2 enterobacter, 3 serratia, 3 staphylococcus aureus and 11 pseudomonas. m ...19863534729
[in vitro activity of a new 3d-generation cephalosporin, cefodizime, on hospital bacteria].minimal inhibitory concentrations (mics) of cefodizime were evaluated by agar dilution for 746 bacterial strains isolated in two hospitals. for enterobacteriaceae mics ranged from 0.008 micrograms/ml to more than 128 micrograms/ml (mode mic: 0.25); mode mics varied across species, ranging from 0.016 micrograms/ml for proteus mirabilis to 1 microgram/ml for citrobacter; mics ranged from 0.12 to 8 for most enterobacter and from 1 to 64 for serratia. the rare cefotaxime-resistant strains, most of w ...19863534743
the combination of pivampicillin and pivmecillinam versus pivampicillin alone in the treatment of acute pyelonephritis.96 patients with clinical symptoms of acute pyelonephritis were randomized to 2 weeks treatment with either a fixed combination of pivampicillin and pivmecillinam or to pivampicillin alone. if needed, treatment was first started with the respective parenteral equivalents of the drugs. acute pyelonephritis was bacteriologically verified in 57 patients, in whom escherichia coli was isolated in 80% of the cases, klebsiella in 7% and proteus mirabilis in 5%. 22 of the 39 patients excluded did not ha ...19863535049
comparison of cefixime and co-trimoxazole in acute uncomplicated urinary tract infection. a double-blind general practice study.five hundred and twenty-eight patients with presumptive acute uncomplicated urinary tract infection (uti) were randomly assigned to receive cefixime 400 mg once daily, cefixime 200 mg twice daily or co-trimoxazole 2 tablets twice a day for 10 days; 477 completed at least 5 days of therapy. of the patients 342 (65%) had positive baseline urine cultures, yielding 353 pathogens. a microbiological response was determined for 280 pathogens (79%), eradication being observed in over 94% of isolates; 15 ...19863535127
antimicrobial prophylaxis in urologic surgery.nosocomial urinary tract infections represent approximately 40 per cent of all nosocomial infections, thereby contributing considerably to secondary bacteremia and sepsis and possibly increased mortality. urethral catheterization is the single most important predisposing factor in the development of urinary tract infection. patients at increased risk of nosocomial infectious complications are the elderly, malnourished and debilitated, those with diabetes or prosthetic devices, and those on immun ...19863535203
magnesium-dependent plaque formation by bacteriophage p1cinc(-) on escherichia coli c and shigella sonnei.phage p1c(-), in a state of the phage not infective to escherichia coli k12, was able to form plaques on a wild-type strain of e. coli c and on shigella sonnei in the presence of mg2+. citrobacter freundii, enterobacter aerogenes, and a salmonella typhimurium gale mutant were not lysed by, but were lysogenized with p1cinc(-), whereas klebsiella pneumoniae, proteus rettgeri, and s. typhimurium lt2 were not susceptible to either p1cinc(-) or p1cinc(+). the lipopolysaccharide structure of e. coli c ...19863535235
[infection of a non-traumatic, anticoagulant-induced retroperitoneal hematoma caused by contiguity with a pyonephrosis. cure by nephrectomy]. 19863535615
aerobic degradation of choline by proteus mirabilis: enzymatic requirements and pathway.cleavage of choline to trimethylamine and acetaldehyde by extracts of proteus mirabilis requires both particulate and soluble protein fractions, k+, and a bound divalent metal cation. the reaction shows a long lag period, abolished only by preincubation of the particulate fraction in the complete reaction system. the two-carbon fragment produced is acetaldehyde; choline cleavage appears to be tightly coupled to dismutation of the acetaldehyde to ethanol and acetate, as indicated by stimulation b ...19863536045
[interactions of ceftriaxone with beta-lactamases including those which hydrolyze cefotaxime].as it occurs with most of 3rd generation cephalosporins, ceftriaxone has few, if any, interactions with penicillinase-type beta-lactamases, such as tem-1, tem-2 or pit-2. these poor interactions are characterized by an extremely low hydrolysis, associated to a poor affinity of these compounds for the penicillinases. conversely, ceftriaxone, as cefotaxime, shows a high affinity for chromosomally-mediated cephalosporinases (indole-positive proteus, enterobacter, pseudomonas...), which is character ...19863537935
properties of an inducible beta-lactamase from proteus vulgaris.the inducible beta-lactamase of the clinical proteus vulgaris isolate 4917/81 was highly purified by column chromatography and by fplc (cation ion exchange column). molecular weight of the enzyme amounted 33,000 daltons, as revealed by sds-electrophoresis. the enzyme was not inhibited by p-chloromercuribenzoate, but by low concentrations of oxacillin and clavulanic acid. the enzyme inactivated not only penicillin derivatives (including ureidopenicillins), but also first-generation cephalosporins ...19863538717
histochemical and biochemical urease localization in the periplasm and outer membrane of two proteus mirabilis strains.proteus mirabilis, a gram-negative bacillus, is often implicated in the formation of infectious kidney stones. as ureolytic activity of this organism is thought to play a major role in its pathogenesis, we adapted our recently described urease localization technique to visualize urease activity in vivo. urease activity was ultrastructurally localized in two clinically isolated p. mirabilis strains by precipitating the enzymatic reaction product (ammonia) with sodium tetraphenylboron. subsequent ...19863539291
speciation, serotyping, antimicrobial sensitivity and plasmid content of proteeae from the environment of calf-rearing units in south west england.a survey was undertaken of the occurrence, serotype, antimicrobial sensitivity and plasmid content of members of the tribe proteeae in the environment of two calf-rearing units in the county of avon in south west england. examples of the following species were found: proteus mirabilis, prot. vulgaris, prot. vulgaris biogroup 2, morganella morganii, providencia stuartii, prov. alcalifaciens and prov. rettgeri. a wide range of serotypes was found, many having been previously reported from nosocomi ...19863540110
[basic and clinical studies of cefotiam in neonates and premature infants].the effect of cefotiam (ctm) on neonates and premature infants was examined in basic and clinical studies. minimum inhibitory concentrations of ctm against 190 clinically isolated strains kept by this department were investigated. this drug was found to have a strong antibacterial effect against escherichia coli, klebsiella spp., proteus mirabilis and streptococcus agalactiae, staphylococcus aureus and staphylococcus epidermidis, although some strains were resistant. the ctm was given to 0-3, 4- ...19863540358
[possibility of fortifying milk nutrient mixtures for children with lactoglobulin against e. coli and proteus].sweet milk nutrient mixtures, biologically active additives (baa) and lactic acid ferments, intended for child nutrition, were enriched with the immune preparation lactoglobulin against e. coli and proteus. serological and immunochemical investigations of hemagglutinating activity and other characteristics of lactoglobulin in the enriched milk mixtures and baa evidenced that the preparation retained its full value during the technological process and in the period of storage. no signs of lactogl ...19863541377
lipopolysaccharides of flagellated and non-flagellated proteus vulgaris strains.lipopolysaccharides from two strains of proteus vulgaris were analyzed. one strain (08) was motile, giving swarming growth on solid media and the other (04)--non-flagellated, not able to swarm. both lipopolysaccharides appeared to be heterogeneous and were separated into two fractions each. yield, chemical composition and sds-polyacrylamide gel electrophoresis showed differences between fractions in percentage content of 0-specific and r-core polysaccharides. relation between ability to swarming ...19863541843
epidemiological study of surgical wound infections.the present report describes the results obtained in an epidemiological study of surgical wound infections through a continuous and active epidemiological surveillance program lasting one year and which covered all the surgical services of the university clinical hospital. the number of infections studied was 217, with an overall infection rate of 3%. the majority of the infections (46.5%) were detected in the general surgery service. regarding the infection rate by services, the highest corresp ...19863542557
[experimental study of the protective activity of the plasma from donors immunized with an enterobacterial vaccine]. 19863542696
[survival of proteus vulgaris in soil contaminated by diesel fuel]. 19863542728
ciprofloxacin therapy in complicated urinary tract infections caused by pseudomonas aeruginosa and other resistant bacteria.thirty courses of ciprofloxacin were given to 28 patients with complicated urinary tract infections mainly caused by pseudomonas aeruginosa. all patients had severe underlying abnormalities and infection usually followed urethral catheterization. the infecting organisms were eradicated from the urine of all patients during therapy and 89% were still clear five to nine days after treatment. at one month follow up the cure rate had fallen to 64%. an increase in resistance to ciprofloxacin was seen ...19863542943
cloning of genes from members of the family enterobacteriaceae with mini-mu bacteriophage containing plasmid replicons.an in vivo cloning system that uses derivatives of the escherichia coli bacteriophage mu with plasmid replicons has been extended to five different species of the family enterobacteriaceae. mu and these mini-mu replicon elements were introduced into strains of e. coli, shigella flexneri, salmonella typhimurium, citrobacter freundii, and proteus mirabilis by infection, by transformation, or by conjugation with newly constructed broad-host-range plasmids containing insertions of these elements. ly ...19873542967
[antibacterial activity of cephem antibiotics against isolates from clinical specimens at the yokohama city university hospital].minimum inhibitory concentrations (mics) of cephem antibiotics against 405 strains belonging to 17 species of clinical isolates were investigated using the standard method of the japanese congress of chemotherapy. the results obtained are summarized below. cephem antibiotics showed weak antibacterial activities against enterococcus sp., b. fragilis and s. marcescens. s. pneumoniae, s. agalactiae, e. coli, k. pneumoniae and p. mirabilis were susceptible to cephem antibiotics. cephem antibiotics o ...19863543429
[iconographic rubric. cerebral abscess caused by proteus in a 3-week-old infant]. 19863545127
roles of antibody and complement in the bactericidal activity of mouse peritoneal exudate neutrophils.the contributions of complement and antibody to phagocytosis and, as a separate process, intracellular killing of proteus mirabilis, were investigated using mouse peritoneal exudate neutrophils. phagocytosis of p. mirabilis was promoted by both immune mouse (ims) and normal mouse (nms) sera. opsonization by ims promoted significantly greater phagocytosis than did nms, as did nms compared with heated ims (hims). the ability of nms to opsonize p. mirabilis for both phagocytosis and phagocytic kill ...19873545276
ciprofloxacin: a comparative evaluation of its bactericidal activity in human serum against four enterobacterial species.serum bactericidal titres following a 200 mg i.v. dose of ciprofloxacin were measured in healthy volunteers and compared with those achieved with standard doses of ceftazidime, piperacillin and gentamicin, given alone or in combination. five strains of each of four enterobacterial species were included in the study. bactericidal titres 1 h after infusion of ciprofloxacin exceeded 1:16 for escherichia coli, klebsiella pneumoniae, proteus vulgaris and enterobacter cloacae. while ceftazidime produc ...19863545730
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