Publications

TitleAbstractYear
Filter
PMID
Filter
[indoor environmental contamination of a trauma hospital by opportunistic gram-negative microorganisms and an evaluation of methods for their detection]. 19863082722
the diffusion of pefloxacin into bone and the treatment of osteomyelitis.pefloxacin was evaluated in the treatment of bone infections. a clinical trial was performed in 15 patients with chronic osteitis (5 staphylococcus aureus, 5 pseudomonas aeruginosa, 3 serratia sp., 1 proteus mirabilis, and a mixed infection with a streptococcus faecalis and escherichia coli). patients were given pefloxacin 400 mg 12-hourly iv for 48 h followed by oral treatment. bone biopsies from the iliac crest were carried out after at least seven days treatment, 2 h after the last dose. seru ...19863086280
characterization of pathogenic bacteria by automated headspace concentration-gas chromatography.automated headspace concentration-gas chromatography (ahc-gc) was used to profile the volatile metabolites produced by proteus mirabilis, klebsiella pneumoniae, pseudomonas aeruginosa and staphylococcus aureus. bacterial cultures were incubated in trypticase soy broth and examined at 24 h. the profiles were consistent for each genus examined and variation observed among the different strains of each species was chiefly quantitative. the volatiles were identified by concurrent headspace concentra ...19863086354
antibodies to the glutamate dehydrogenase of plasmodium falciparum.polyclonal antisera raised against plasmodium knowlesi reacted with nadp-specific glutamate dehydrogenase (gldh) of p. knowlesi, gldh of p. falciparum and gldh of proteus spp. the antisera did not react with nad(p) gldh from bovine liver. polyclonal antisera raised against the gldh of proteus spp. cross-reacted with gldh from p. falciparum. monoclonal antibodies (mcabs) obtained from mice immunized with proteus gldh were either specific for the bacterial enzyme or cross-reacted with p. falciparu ...19863086819
torsed infected dermoid cyst with concurrent ectopic pregnancy. 19863085005
ofloxacin: bactericidal effect in an in vitro pharmacokinetic model.ofloxacin has been evaluated in an in vitro model where microorganisms were exposed to the varying concentrations met in human serum after the oral administration of a 200 mg dose. the recently isolated strains were selected with minimum inhibitory concentrations (mics) representative of the mics 90% of ofloxacin against the species considered. results showed that ofloxacin sterilized the bacterial cultures of s. aureus, p. morganii and e. coli, with 99.99% of killing still evident for p. aerugi ...19863085961
in-vitro activity of pefloxacin compared to other antibiotics.pefloxacin is a new quinolone carboxylic acid with a broad spectrum of antibacterial activity. a comparison was made of the in-vitro activity of pefloxacin and that of nine other antibiotics (ampicillin, ticarcillin, piperacillin, cefazolin, cefotaxime, ceftazidime, gentamicin, amikacin and norfloxacin). the mic90 of pefloxacin against 500 strains of enterobacteriaceae ranged from 0.25 mg/l (escherichia coli, indole + proteus spp., enterobacter cloacae, salmonella spp. and shigella spp.) to 1 mg ...19863086279
[preventive properties of the blood sera from persons vaccinated with a proteus vaccine made from soluble antigen complexes].in experiments of the passive protection of mice the protective properties of sera obtained from humans before and after their immunization with proteus vaccine used as a monopreparation or in combination with staphylococcal toxoid and/or pyoimmunogen were studied. when introduced in a single subcutaneous injection, proteus vaccine prepared from soluble antigenic complexes ensured an increase in the protective properties of sera. the second injection of the vaccine essentially enhanced the prote ...19863087123
resistance of providencia stuartii to chlorhexidine: a consideration of the role of the inner membrane.spheroplasts of three strains of providencia stuartii (one sensitive, one moderately sensitive and one resistant to chlorhexidine) were induced by cefoxitin, glycine or a lysozyme-tris-edta combination, and their susceptibility to chlorhexidine-induced lysis investigated. maximum lysis of spheroplasts occurred at a low (2.5-5 micrograms/ml) concentration of chlorhexidine and was greatest with the most sensitive strain, pv2. the possible role of the inner membrane in chlorhexidine resistance is c ...19863087933
in vitro evaluation of a-56619 (difloxacin) and a-56620: new aryl-fluoroquinolones.the in vitro antibacterial potencies of a-56619 and a-56620, two new aryl-fluoroquinolones, were compared with the potency of norfloxacin against a broad spectrum of organisms. cefotaxime, aztreonam, piperacillin, imipenem, penicillin, and gentamicin were also tested for reference purposes. the mics required to inhibit at least 90% of the strains tested ranged from 0.25 to 4 micrograms/ml for a-56619 and from 0.06 to 0.5 microgram/ml for a-56620 for members of the enterobacteriaceae. a-56619 was ...19863087274
survey of aminoglycoside resistance patterns.we studied the susceptibility of 545 gram-negative clinical isolates to six aminoglycosides: gentamicin, tobramycin, netilmicin, amikacin, 2'-n-ethylnetilmicin, and 6'-n-ethylnetilmicin. the resistant strains (359) have been classified into aminoglycoside resistance (patterns) phenotypes (agrps) corresponding to known resistance mechanisms. the results clearly demonstrated that most strains of proteus spp., klebsiella spp., escherichia coli and pseudomonas aeruginosa appeared to be able to adeny ...19863087636
[immunoglobulins in the serum of chronic pyelonephritis patients].the authors studied the serum levels of immunoglobulins g, a and m in 72 patients with chronic pyelonephritis and 77 clinically healthy subjects. in spite of the high biological variability of the indices studied, high serum levels of igg and iga were established in the patients with active urologic infection and with advanced renal insufficiency. significantly higher values of igg were established in active pyelonephritis, caused by e. coli and proteus and of all immunoglobulins--in infection w ...19863087065
aminoglycosides plus beta-lactams against gram-negative organisms. evaluation of in vitro synergy and chemical interactions.combination antibiotic therapy has been used mainly to broaden the antibacterial spectrum and prevent the development of resistance. antibiotic combinations proven to be synergistic in vitro are associated with a significantly better in vivo response, particularly in the compromised host in whom traditional treatment combines an antipseudomonal penicillin plus an aminoglycoside. several investigators have examined combining new agents, such as the third-generation cephalosporins (cefotaxime, cef ...19863088998
review of epidemic aminoglycoside resistance worldwide.epidemic aminoglycoside resistance may be caused by the spread of a species with distinctive chromosomal genes (e.g., pseudomonas aeruginosa), or it may be due to the dissemination of plasmids or transposons between genera. although strains of p. aeruginosa resistant to aminoglycosides because of impermeability may cause nosocomial outbreaks, most of the acute increases in aminoglycoside resistance are due to the spread of inactivating enzymes by plasmids. the index species for intergeneric outb ...19863089005
the sensitivity of various serologic tests in the diagnosis of rocky mountain spotted fever.sensitivity indices for various serologic tests in the diagnosis of rocky mountain spotted fever (rmsf) were determined from rmsf surveillance data collected at the centers for disease control (cdc) during 1981-1984. during these 4 years, a total of 4,141 cases of rmsf in the u.s. were reported to cdc. case report forms, which include information on dates and results of serologic testing, were received for 3,567 of these cases; 1,774 were laboratory-confirmed according to criteria published prev ...19863089042
in vitro activity of aztreonam against gram negative bacteria from clinical specimens and its comparison with other commonly used antibiotics.a total of 755 gram negative bacteria isolated from clinical specimens were tested against aztreonam by the disc agar diffusion test. the strains of bacteria used in this study consisted of escherichia coli (314), enterobacter aerogenes (30), e. agglomerans (7), e. cloacae, (39), citrobacter diversus (9), c. freundii (13), hafnia alvei (3), acinetobacter calcoaceticus (10), klebsiella oxytoca (6), k. ozaenae (5), k. pneumoniae (107), morganella morganii (3), moraxella sp. (10), pasteurella multo ...19863088346
parapharyngeal abscess: an unusual complication of aural cholesteatoma. 19863088530
[hospital infection due to pseudomonas aeruginosa in resuscitation departments].the bacteriological survey of resuscitation and intensive care units has revealed the presence of p. aeruginosa strains in the microflora in 69.4% of cases. the circulation of 1-2 p. aeruginosa strains, identical in their serovar and pyocin type, is indicative of the presence of hospital infection and, therefore, the endogenous character of the contamination of patients. p. aeruginosa hospital strains are the main causative agents of infectious complications in the patients treated in resuscitat ...19863088875
preoperative swabs for the treatment of draining ears after middle ear surgery.the value of a preoperative swab for the treatment of postoperative infection after middle ear surgery was investigated. in a selected group of 80 patients with postoperative infection a preoperative swab was available, and the variability was analysed for each species. the well-known pathogens pseudomonas aeruginosa, staphylococcus aureus, proteus and escherichia coli showed good correlation between pre- and postoperative swabs. in contrast to this all other species found in preoperative swabs ...19863089648
pattern recognition analysis of in vivo enzyme-substrate fluorescence velocities in microorganism detection and identification.a spectrometric technique is presented that combines most of the important criteria necessary for efficient detection and identification of microorganisms. these criteria include simplicity of experimental design, various degrees of sensitivity and selectivity, convenience, and total reaction times of less than 15 min. the study takes advantage of the inherent extracellular enzymes present in living as opposed to dead, non-enzyme-producing organisms. sequentially these are harnessed in in vivo r ...19863089149
expression of the proteus mirabilis reca gene in bacillus subtilis is directed by its own promoter.the reca gene of proteus mirabilis (recapm) has been cloned into the psti site of the bacillus promoter-probe plasmid ppl603. when present on this plasmid, the recapm1) gene is expressed in b. subtilis under the control of its own transcriptional and translational signals. it is concluded that the high at-content of the dna sequence upstream of the -35 region is of decisive importance for the usage of the recapm promoter by the b. subtilis rna polymerase. the results are discussed in relation to ...19863090230
characterization of a protein from normal human polymorphonuclear leukocytes with bactericidal activity against pseudomonas aeruginosa.purification of a bactericidal protein (bp) from the cytoplasmic granules of normal human polymorphonuclear leukocytes (pmn) with activity against pseudomonas aeruginosa is described. bactericidal activity from acid extracts of a mixed granule population was purified 175-fold by a two-step chromatographic procedure. the first step, dye-ligand affinity chromatography with matrex-gel orange a, was followed by cation-exchange chromatography with bio-rex 70 resin, and this combination routinely gave ...19863093381
growth of salmonellas in different enrichment media.the usefulness of selenite-f (s-f), tetrathionate (mkt) and rappaport-10 (r-10) broths as enrichment media to support growth of salmonellas either alone or in the presence of other competing organisms was studied. their ability to support the growth of stressed salmonellas from water was also investigated. it was observed that r-10 was more inhibitory to competing organisms than mkt and s-f. it strongly inhibited the growth of pseudomonas aeruginosa, citrobacter freundii and proteus vulgaris tho ...19863093439
comparison of bacteria in the tympanic cavity and the mastoid antrum in chronic otitis media.materials collected from the tympanic cavity before operation and from the mastoid antrum during operation of 58 discharging ears of patients with chronic otitis media were cultured, and the bacteria in these two cavities were compared. staphylococcus aureus was the commonest organism in the tympanic cavity, and s epidermidis in the mastoid antrum. anaerobic bacteria were found only in the mastoid antrum of patient with cholesteatoma. in 32 (55 per cent) of the 58 ears examined, bacteria were de ...19863092687
a survived case of diabetic nonclostridial gas gangrene and the review of the literatures on microbiological findings.nonclostridial gas gangrene in diabetics results in poor prognosis. in this paper, a survived case of diabetic nonclostridial gas gangrene caused by proteus vulgaris and streptococcus faecalis is described and microbiological findings of 31 cases of the literatures including our own case were reviewed. twenty of 31 cases died (64.5%). the microbiological findings in 30 cases revealed an average of 1.8 organism per case. predominant aerobes were e. coli (56.7%), streptococcus faecalis (16.7%), ae ...19863091889
the proteus syndrome: the elephant man diagnosed.sir frederick treves first showed joseph merrick, the famous elephant man, to the pathological society of london in 1884. a diagnosis of neurofibromatosis was suggested in 1909 and was widely accepted. there is no evidence, however, of café au lait spots or histological proof of neurofibromas. it is also clear that joseph merrick's manifestations were much more bizarre than those commonly seen in neurofibromatosis. evidence indicates that merrick suffered from the proteus syndrome and had the fo ...19863092979
transferable resistance to cephalosporins, ureidopenicillins and trimethoprim.transferable resistance to classical cephalosporins emerged three years ago among enterobacteriaceae simultaneously in several hospitals and was associated with resistance to other betalactam drugs, i.e. with a transmissible tem-like betalactamase. a systematic survey of incidence of r plasmids in proteus sp. lead, in two district phls, to demonstration of transferable azlocillin resistance. this resistance was most frequently associated with that for carbenicillin, but also strains susceptible ...19863095072
comparison of effectiveness of aminoglycosides and cephalosporins in gram-negative rods.aminoglycosides and cephalosporins belong among antimicrobials with a pronounced effect on most gram-negative rods. in vitro susceptibility and resistance to gentamicin, tobramycin, amikacin, netilmicin, cefoxitin, cefotaxime, cefsulodin, cefoperazone in klebsiella pneumoniae, enterobacter spp., proteus mirabilis, escherichia coli and pseudomonas aeruginosa was tested by the quantitative dilution micromethod. selected, highly susceptible strains of the above species were furthermore compared for ...19863095073
aztreonam in the treatment of severe urinary tract infections in pediatric patients.aztreonam was administered to 30 patients, ages 0.03 to 15.4 years, with severe and in 21 cases complicated urinary tract infections caused by members of the family enterobacteriaceae and pseudomonas aeruginosa which were resistant to ampicillin and susceptible to the study drug in vitro. a mean dose of 47.7 mg/kg was given intramuscularly every 12 h to 26 patients. in four patients with renal insufficiency, the dose was reduced according to pharmacokinetic data. permanent urine sterilization an ...19863094442
do clinical microbiology laboratories report complete bacteriology in urine from patients with long-term urinary catheters?bacteriuria associated with long-term urinary catheters (those in place for greater than or equal to 30 days) appears to be the most common nosocomial infection in u.s. medical care facilities. this bacteriuria is polymicrobial and dynamic and accompanied by fevers, catheter obstructions, bacteremias, and deaths. we compared the reporting by our research laboratory of bacteria present in urine from long-term-catheterized nursing home patients with that by two commercial laboratories. the commerc ...19863093528
pseudallescheriasis in northern britain.five cases of pseudallescheria boydii infection are reported from the northern part of the united kingdom. none of the patients had travelled abroad. three cases of otitis in which p. boydii was associated with bacterial infections were diagnosed within a 6-month period. a lady who had diabetes mellitus presented with a pulmonary fungus ball in a pre-existent cavity caused by a previous bacterial infection. after minor trauma at the site of an old skin graft, a farmer's wife developed an ulcer o ...19863097296
microbiological basis for the use of fosfomycin trometamol as single-dose therapy for simple cystitis.fosfomycin trometamol (fot), a new soluble salt of fosfomycin, was developed especially for single-dose treatment in uncomplicated urinary tract infections. in this study, the minimum inhibitory concentrations (mics) of fot were measured both in nutrient broth and human urine and compared with calcium fosfomycin, pipemidic acid and cotrimoxazole. a total of 300 bacterial strains of different species from recent urinary infections were studied. staphylococcus aureus showed the lowest mic (0.38 mi ...19863094965
[aerobic bacterial flora of the nasal cavity of rabbits].on the basis of bacteriological examinations carried out in april 1984 on 60 intranasal swabs, aerobic respiratory microbes were studied in rabbits. differences in flora between animals with and without respiratory diseases were studied. fourteen bacterial species were identified with no difference due to the pathological status. they were: bordetella bronchiseptica, staphylococcus epidermidis, streptococcus faecalis, pasteurella multocida, staphylococcus aureus, bacillus sp., branhamella catarr ...19863096189
[in-vitro activity of cefonicid on hospital bacteria. regression line and proposal for critical values].antimicrobial activity of cefonicid, a new second generation cephalosporin, against 315 hospital isolates (4th trimester 1984) was investigated. e. coli and proteus mirabilis were the most susceptible species. all e. coli strains except one were inhibited at 8 mg/l (modal mic: 0.5); mics of all indole + proteus were 8 mg/l (modal mic: 0.06). another group was moderately susceptible: mics of klebsiella and citrobacter ranged from 0.12 to 128 mg/l, but mics of 50% of these strains were less than o ...19863095773
[a clinical bacteriological efficacy study on a fosfomycin otic solution].fosfomycin (fom) otic solution was administered to 587 patients with suppurative otitis media infections including 190 patients in the dose-establishment test, 126 patients in the open clinical trial and 271 patients in the double blind test. various bacteria were detected in the 549 cases in which bacteriological investigation was possible. main bacteria detected from the above cases were s. aureus (261 strains, 47.5%), p. aeruginosa (93 strains, 16.9%), coagulase negative staphylococci (cns) ( ...19863099028
[does radiosterilization of the vagina occur through therapeutic radiation dosages?].the endogenous genital flora is a major source of infections of the female genital tract, especially in patients with cervical or endometrial cancer. until recently the "radiosterilisation of the vagina", respectively a prophylactic and/or therapeutic effect of irradiation (external high voltage as well as intracavitary-radium) concerning infections was postulated in the literature. this theory was disproved in a prospective clinical and bacteriological study covering 48 patients with advanced c ...19863097858
serological cross-reactions between rickettsia typhi, proteus vulgaris ox19, and legionella bozemanii in a series of febrile patients.the sera of 19 patients with a febrile disease of undetermined etiology were positive in the indirect immunofluorescence assay (ifa) to legionella bozemanii serogroup 1 (lb) and rickettsia typhi (rt). to both antigens, high titers of igg-class and igm-class antibodies were demonstrated. several of the patients also had positive ifa and weil-felix reactions to proteus vulgaris ox19 (px 19). a sharp reduction of the serotiters to all three antigens was achieved by absorption of the sera with any o ...19863098699
reversal of the surface effects of chlorhexidine diacetate on cells of providencia stuartii.chlorhexidine diacetate (cha) increased the hydrophobicity of the cell surface of cells of three strains of providencia stuartii. removal of at least some of the cha from the cells by washing them with an appropriate antidote partially reversed the hydrophobicity-increasing action of the biguanide. the effects of other treatments on cell surface hydrophobicity were examined with these strains and, for comparison, with two strains each of escherichia coli and pseudomonas aeruginosa: ethylenediami ...19863100485
in vitro activity of sch-34343, a new penam, and other antimicrobial agents against clinical isolates from cancer patients.the in vitro activity of sch-34343, a new penam antibiotic, was tested against gram-positive and gram-negative isolates from cancer patients, and compared to that of 7 other antimicrobial agents. sch-34343 was extremely active against the enterobacteriaceae with mic90 ranging from 0.39 to 6.25 micrograms/ml for citrobacter spp., enterobacter spp., escherichia coli, klebsiella spp., proteus spp. and serratia marcescens. it was less active against acinetobacter spp. (mic90 6.25-12.5 micrograms/ml) ...19863100147
[guidelines for testing and rating hand decontamination preparations]. 19863101326
in vitro activity of ofloxacin against 210 clinical isolates of typhoid salmonellae.210 clinical isolates of typhoid salmonellae, cultivated from blood of patients with typhoid fever (108 strains of salmonella typhi, 99 strains of salmonella paratyphi a and three strains of salmonella paratyphi b) as well as 266 random clinical isolates of escherichia coli (n = 152), klebsiella spp. (n = 51) and proteus spp. (n = 63) were tested for susceptibility to ofloxacin, using an agar dilution method. all the typhoid salmonellae were found to be highly sensitive to ofloxacin with mics ra ...19863102385
interactions of antimicrobial agents and antineoplastic agents.the in-vitro interactions of four antimicrobial agents (piperacillin, dibekacin, minocycline, norfloxacin) and four antineoplastic agents (mitomycin c, bleomycin, doxorubicin, 5-fluorouracil) were examined by the chequerboard dilution method using 108 clinical isolates of four species of gram-negative bacilli. among the antimicrobial agents, piperacillin showed the greatest degree of synergism and norfloxacin the least. the frequency of synergism was essentially similar among the antineoplastic ...19863102447
antibacterial properties of imipenem with special reference to the activity against methicillin-resistant staphylococci, cefotaxime-resistant enterobacteriaceae and pseudomonas aeruginosa.imipenem was examined with standardized agar dilution procedures against a wide range of bacteria. geometric mean mics against the genera escherichia, klebsiella, enterobacter, citrobacter and serratia were 0.1-0.4 mg/l, and proteus and providencia spp. were inhibited by 0.25-4 mg/l. acinetobacter calcoaceticus var. anitratum strains were inhibited by concentrations ranging from 0.12-0.5 mg/l. methicillin-susceptible staphylococci were highly susceptible to the drug (mics: less than or equal to ...19863102452
ceftazidime, a broad spectrum cephalosporin with activity against ps. aeruginosa.ceftazidime is one of the oximinoaminothiazolyl cephalosporins with resistance to most b-lactamases from gram-negative bacteria, and a very wide spectrum of activity including ps. aeruginosa. mic's of less than 0.1 mg/l are seen routinely against e. coli, k. pneumoniae, e. cloacae, proteus spp. both indole positive and negative, serratia sp., and providence sp. the mean mic against clinical isolates of ps. aeruginosa is less than 2 mg/l. it is bactericidal at concentrations close to the mic and ...19863100612
[comparative studies of antimicrobial agents against causative organisms isolated from urinary tract infections (1984). iii. secular changes in susceptibility].cases with infections of urinary tracts were divided into 3 groups of the simple infections, and complicated infections without indwelling of catheter, and complicated infections with indwelling of catheter. susceptibilities to antimicrobial agents of escherichia coli, klebsiella spp., proteus spp., citrobacter spp., enterobacter spp., pseudomonas aeruginosa and serratia marcescens which were isolated from patients with these infections were determined. there was no tendency of decline in the su ...19863102809
selection of beta-lactamase producers during cephalosporin and penicillin therapy.treatment failures due to beta-lactamase producing strains of enterobacter cloacae, proteus vulgaris, citrobacter freundii and pseudomonas aeruginosa are frequently reported. it is difficult, however, to determine the underlying mechanism of resistance development. different beta-lactam drugs, such as cephalosporins and penicillins have different ecological impacts on the infections and physiological flora of patients. they select in different ways although the mutation frequency of strains towa ...19863103207
[comparison of in vitro activities of first, second and third generation cephem antibiotics against various pathogens isolated from clinical material in 1985].in vitro susceptibilities of 2,133 strains of various pathogens isolated from clinical materials in 1985 to various cephem antibiotics were studied using the showa disk diffusion test. the following antibiotics were evaluated: cephalexin (cex), cephalothin (cet), cefazolin (cez), cefotiam (ctm), cefoxitin (cfx), cefmetazole (cmz), cefotaxime (ctx), cefoperazone (cpz), ceftizoxime (czx), cefmenoxime (cmx), latamoxef (lmox) and cefsulodin (cfs). s. aureus: susceptible strains to cet, cez, ctm, cfx ...19863100836
[comparative studies of antimicrobial agents against causative organisms isolated from urinary tract infections (1984). i. susceptibility distribution].our research group was engaged for 3 years (1979-1981) in a study on sensitivities to antibiotics of 4 bacterial groups including representative pathogenic bacteria found in cases of urinary tract infections; i.e. e. coli, klebsiella spp., citrobacter spp., and proteus spp. since 1982, all the bacterial strains isolated by our group from patients with urinary tract infections and deemed by doctors in charge as pathogens were sent to the laboratory of clinical pathology of juntendo university, wh ...19863102808
[bactericidal activity of beta-lactams and aminoglucosides: comparative evaluations].in this study we have evaluated the bactericidal activity kinetics of some cephalosporins, ureidopenicillins and aminoglycosides against 41 gram negative clinical isolates. aminoglycosides, particularly gentamicin, showed the better bactericidal activity which appeared already at the second-third hour after the addition of the antibiotic. the bactericidal activity of cefotaxime, ceftazidime and cefotetan was remarkable but it appeared only at the sixth-eight hour. this difference was statistical ...19863103652
in vitro antibacterial activity of neem oil. 19863102364
in-vitro bacterial killing kinetics of ticarcillin/clavulanic acid.an in-vitro model was developed to study the rates of killing by ticarcillin/clavulanic acid combinations of various beta-lactamase producing, ticarcillin resistant, logarithmic phase clinical isolates. killing, defined as a 3 log reduction, was dependent on the organism, the concentration of clavulanic acid and the duration of exposure. for most isolates studied an optimum period of exposure to and concentration of clavulanic acid could be defined. certain test strains showed optimum response t ...19873106304
serum bactericidal activity of ciprofloxacin and ofloxacin in volunteers.the serum bactericidal activity of two newer quinolones, ciprofloxacin and ofloxacin, against 206 clinical bacterial isolates was determined in six male volunteers after oral administration of either 500 mg of ciprofloxacin or 200 mg of ofloxacin respectively. the highest bactericidal titers were achieved against enterobacteriaceae 1 h after ciprofloxacin administration, ranging from 1:121 for indole-positive proteus species to 1:30 for serratia spp. ofloxacin generated lower titers, ranging fro ...19873106034
[treatment of urinary tract infections with solcourovac].urinary tract infections are among the most common infections found in women. if recurrences or reinfections become frequent treatment can be problematic. immunisation of the patients by vaccinating them against urinary tract pathogens offers a possibility of managing these infections. in clinical trials we tested solcourovac, a new vaccine to counteract bacterial pathogens, with regard to efficacy, frequency of recurrence, and tolerance. 113 of the 118 patients included in this study with bacte ...19873106132
[escherichia coli, coliform bacteria, pseudomonas aeruginosa, proteus and staphylococcus aureus carriers among children 5-15 years old].the throat swap cultures of 250 children between 5-15 years ages examined for the microorganisms, e. coli, coliform, protus, ps. aeruginosa and s. aureus. 66 (26.4%) e. coli, 64 (25.6%) s. aureus, 9 (3.6%) coliform, 3 (1.2%) ps. aeruginosa, 2 (0.2%) proteus organisms were isolated from throat swaps cultures of 144 (57.6%) children. 106 (42.4%) children had contained normal oral flora. s. aureus and e. coli strains were tested using disk method for the sensitivity of some antibiotics. s. aureus s ...19863104734
bacterial isolation from and transmission by boophilus decoloratus and boophilus geigyi.bacteria were isolated from the haemolymph of boophilus decoloratus and boophilus geigyi which had engorged on trade cattle in nigeria. all the genera of the bacteria were also isolated from the eggs which they laid although some of them were missing from the larvae which eventually hatched. when clean rabbits were inoculated with some genera of bacteria and clean, laboratory bred larvae of b. decoloratus and b. geigyi fed on them, the same genera of bacteria were subsequently isolated from the ...19873108116
somatic evolution of diversity among anti-phosphocholine antibodies induced with proteus morganii.the variable region sequences of light and heavy chains (vl and vh) were determined for 11 hybridoma antibodies produced in response to the pc moiety on proteus morganii. these hybridomas were derived from two separate fusions, one obtained from mice early in a secondary response and the other from late in a secondary response. all of these antibodies possessed a cross-reactive idiotype found on anti-pc antibodies in the m603 family, and exhibited preferential specificity for pc in the context o ...19873106498
[bacterial infection agents in hospitalized patients].during six months the pattern of microbial isolates has been analysed from 1492 urinary tract infections, from 1445 wound infections, from 451 bronchial secretions, from 3067 blood cultures, from 116 peritoneal swabs and from 39 cerebrospinal fluids. all the specimens investigated were taken from patients of surgical, neurosurgical, internal wards and two intensive care units. in total, the most frequent isolates were grampositive microorganisms like staphylococus aureus, straphylococcus epiderm ...19863107262
in vitro activity of ro 15-8074 and ro 19-5247.the in vitro activity of ro 15-8074 (cefetamet) and ro 19-5247, new oral cephalosporins, was compared with that of amoxicillin, cephalexin, cefaclor, cefuroxime and erythromycin against 292 clinical isolates using the agar dilution method. both ro 15-8074 and ro 19-5247 were very active against proteus mirabilis, neisseria gonorrhoeae, haemophilus influenzae and streptococcus pyogenes, but less active against staphylococcus saprophyticus and enterobacter cloacae. ro 19-5247 was more active than ...19873109897
reactions of hemocytes of immune and non-immune galleria mellonella larvae to proteus mirabilis.immune larval galleria mellonella removed live proteus mirabilis from the hemolymph less effectively than did non-immune larvae. this was attributed to a decline in total hemocyte counts, levels of plasmatocytes and granulocytes and hemocyte adhesion capacity. immune serum possessed factors which reduced bacterial adhesion to the hemocytes. this was not due to altering bacterial surfaces but rather to irreversible suppression of hemocyte activity.19873109970
post hoc, ergo propter hoc. 19873110192
[pseudomonas aeruginosa and proteus antigenemia and antibody formation in mono- and mixed infections in patients with suppurative inflammatory diseases].p. aeruginosa and proteus antigenemia and antibody production have been studied in 335 patients with purulent inflammatory diseases. the study has revealed that in the association of p. aeruginosa and proteus with staphylococci and representatives of the family enterobacteriaceae the level of antigenemia is considerably lower than in monoinfections or in the association of these microorganisms with streptococci. in mixed infections humoral immune response develops later than in cases of monoinfe ...19873107287
purple urine bag syndrome.purple pigment extracted from the urinary catheters and collecting bags of two elderly female patients was analysed by a variety of chemical techniques, including mass spectroscopy and nuclear magnetic resonance. although previous studies identified the pigment as indigo, we failed to confirm this. our analysis also demonstrated that indicanuria is not a requirement for the production of the pigment and furthermore indicates that the molecular structure of the pigment is either a steroidal or bi ...19873109308
in vitro evaluation of ro 23-6240, a new fluorinated 4-quinolone.the in vitro antibacterial activity of ro 23-6240 was assessed and compared with those of ciprofloxacin and beta-lactam antibiotics including several oral compounds against members of the family enterobacteriaceae (n = 130) and pseudomonas spp. (n = 31). in general, ro 23-6240 was 2 dilution steps less active than ciprofloxacin. for the pseudomonas spp. the mics for 90% inhibition were 2 and 0.5 mg/1 for ro 23-6240 and ciprofloxacin, respectively. for the other species tested, the mic90 values f ...19873109817
nosocomial infectious balanoposthitis in neutropenic patients.we have reported balanoposthitis as a source of fever and bacteremia in two neutropenic uncircumcised patients. the etiologic organisms were pseudomonas aeruginosa in one case and providencia stuartii in the other. in one patient, the diagnosis was delayed by the presence of a condom catheter. this emphasizes the importance of personal hygiene in uncircumcised men about to undergo immunosuppressive therapy, and the need for judicious use of condom catheters in such patients.19873110971
[study of the sensitivity to pipemidic acid, pefloxacin and norfloxacin of 444 bacterial strains isolated at a general hospital].after one year of use of pefloxacin in the intensive care unit, medicine unit and surgery unit and the following recent commercialization without prescription of norfloxacin, we studied for a period of two months (april and may 1986) the susceptibility to pipemidic acid, to pefloxacin and norfloxacin of 444 bacteria strains isolated obtained from clinical specimens. the antibiotic susceptibility is determined by disk diffusion test. we obtained the following results: susceptible methicillin stap ...19873112705
sensititre autoreader for same-day breakpoint broth microdilution susceptibility testing of members of the family enterobacteriaceae.the sensititre autoreader system is an instrument-assisted broth microdilution susceptibility test procedure based on the detection of fluorogenic growth substrate metabolism by test bacteria with different concentrations of antimicrobial agents. in the current investigation, this system was assessed as a means for predicting the in vitro activity of 17 antimicrobial agents versus numerous species of the family enterobacteriaceae and pseudomonas aeruginosa by using a breakpoint broth microdiluti ...19873114321
[effects of loperamide on the fecal flora in children in severe diarrheas].loperamide has been recently indicated in the management of infants with severe protracted diarrhea. the purpose of this study was to determine the effect of loperamide on fecal flora in severe protracted diarrheas. 19 children aged 1 to 36 months, with severe protracted diarrhea were studied: 14 received loperamide (0.5 mg/kg/d) and 5 were without loperamide treatment. criteria analysed were: clinical tolerance (vomiting and abdominal distention); efficacy (number of stools, transit time and na ...19873112712
effect of chlorhexidine/edta/tris against bacterial isolates from clinical specimens.chlorhexidine/edta/tris was more active compared with chlorhexidine against the following species of organisms: acinetobacter species, citrobacter species, enterobacter species, escherichia coli, proteus mirabilis, providence species, pseudomonas species, staphylococcus aureus, staphylococcus epidermidis and streptococcus faecalis, when tested in isosensitest agar. the addition of 20% serum to the medium reduced its effectiveness mainly against providence species, proteus species, and streptococ ...19873116368
[in vitro studies on microbial incorporation of nitrogen from [15n2] urea and [15n]ammonium chloride by human intestinal flora].6 typical bacteria species of the human intestinal flora (e. coli, klebsiella pneumoniae, proteus vulgaris, streptococcus faecalis, bacteroides fragilis, bifidobacterium sp.) were incubated in a liquid medium for 48 h with [15n2]-urea and [15n]-ammonium chloride. the rates of [15n]-incorporation were calculated. they depend reproducible on the species examined, on the kind of the offered npn-substance and on the amount of npn-substance in the medium. with [15n2]-urea the minimal rate of incorpor ...19873116431
a modified kelsey-sykes method for testing disinfectants with 2,3,5-triphenyltetrazolium chloride reduction as an indicator of bacterial growth.different dilutions of chlorhexidine gluconate were tested by the kelsey-sykes procedure. the method was further modified on microtitration plates using 2,3,5-triphenyltetrazolium chloride (ttc) reduction as an indicator of bacterial growth. there was a good correlation with the results based on ttc reduction and the conventional method based on turbidity changes caused by bacterial growth. furthermore, the modified method using ttc reduction is more rapid and can be read by the naked eye becaus ...19873114212
[urinary infection associated with schistosomiasis haematobia]. 19873118434
in vitro antibacterial activity of commonly used antibiotics against urinary tract isolates. 19873114512
overproduction of gene products by the super-high-copy-number plasmid pnr333.the plasmid pnr333 is a kanamycin-resistant, deletion derivative of pnr113 with an extremely high copy number in escherichia coli and in proteus mirabilis. in order to determine the usefulness of pnr333 as a replication gene of vector, the genes encoding chloramphenicol acetyl-transferase (cat) and beta-galactosidase (beta-gal) were cloned individually into both pnr333 and other low-copy-number plasmids. the expression of the cloned genes was compared by measuring the specific activity of each e ...19873119963
subperiosteal and bezold's abscesses complicating cholesteatoma: a case report.a case of subperiosteal and bezold's abscesses that occurred in a 60-year-old man with cholesteatoma has been described and the literature reviewed. bezold's abscess is rarely seen in the current era of antibiotics. the simultaneous occurrence of a subperiosteal abscess in association with cholesteatoma, particularly in an adult, makes this case even more unusual. although antibiotics have reduced the complications associated with acute otitis media, rare complications still occur and should be ...19873120109
acute gangrene of the scrotum and penis in four hematologic patients. the usefulness of hyperbaric oxygen therapy in one case.we describe four cases of fournier's gangrene complicating the postchemotherapy aplastic phase (polymorphonuclear cells [pmn] less than 500/microliter) in three patients with acute leukemia and one patient with non-hodgkin's lymphoma. blood and local cultures from two patients contained pseudomonas aeruginosa, and local cultures from another patient grew both p. aeruginosa and proteus rettgeri. early recognition and aggressive antibiotic and surgical treatment with granulocyte recovery were fund ...19873113713
the diagnosis and management of urinary tract infection in children: a two year study in army general practice.a study of possible new cases of urinary tract infection in a population of some two thousand children under the age of twelve years is described. over a two year period one hundred and four children were seen in the study and seventeen were found to have a significant bacterial infection. presenting symptoms, urinalysis results, investigation and methods of follow-up are examined. the results suggest that the incidence of detectable urinary system abnormality in children presenting for the firs ...19873119825
incidence of inducible beta-lactamases in gram-negative septicemia isolates from twenty-nine european laboratories. european study group on antibiotic resistance.in 1984 the european study group on antibiotic resistance (esgar), which is made up of 29 laboratories in 12 european countries, consecutively collected gram-negative bacilli and staphylococci isolates from blood and using the microdilution method performed susceptibility testing with 11 beta-lactam antibiotics. a total of 2,578 isolates were collected; 68% were gram-negatives and 32% staphylococci. pseudomonas spp. accounted for 12% of the strains, enterobacter spp. 7%, serratia spp. 3%, indole ...19873117535
recovery of beta-lactamase producing bacteria in pediatric infections.the presence of beta-lactamase producing bacteria (beta lpb) was investigated in specimens obtained from 1469 children who presented with infections of the skin and soft tissue (648), upper respiratory tract (514), pulmonary sites (137), surgical sites (113), and other (57). of 4989 bacterial isolates recovered, 910 (18%) were beta lpb, 492 (54%) aerobes, and 418 (46%) anaerobes. the beta lpb were recovered in 751 (51%) of the children. the most frequently recovered beta lpb was staphylococcus a ...19873121158
[susceptibility of clinical bacterial isolates to aztreonam].in vitro activities of 7 antimicrobial agents against organisms (474 strains) isolated from patients with various infections in ehime university hospital from may to july 1986 were investigated. summarized results are as follows: 1. aztreonam (azt) showed potent activities against escherichia coli, citrobacter sp., klebsiella pneumoniae, serratia marcescens, proteus sp., pseudomonas aeruginosa and haemophilus influenzae. 2. antimicrobial activities of azt were especially superior against proteus ...19873121888
assessment of antiseptic bladder washout procedures using a physical model of the catheterised bladder.a simple physical model of the catheterised bladder has been used to assess the activities of antiseptic agents that have been recommended as bladder instillations in the treatment of urinary tract infections in patients with indwelling catheters. the activities of povidone-iodine, phenoxyethanol, chlorhexidine, chlorhexidine supplemented with edta + tris, noxythiolin and neomycin were examined against selected species of uropathogens. except for phenoxyethanol against pv. stuartii and possibly ...19873122868
susceptibility of gentamicin sensitive and resistant gram negative bacilli to ceftazidime.the in-vitro activity of ceftazidime was compared with other third-generation cephalosporins and gentamicin against 409 isolates. ceftazidime was the most active agent against enterobacter sp., proteus mirabilis, serratia sp., proteus sp. and pseudomonas aeruginosa. it was less active against escherichia coli and klebsiella than moxalactam and cefotaxime but more active than cefoperazone. against gentamicin resistant e. coli, klebsiella, enterobacter and p. aeruginosa, ceftazidime was more activ ...19873124067
trimethoprim-sulfamethoxazole in cyst fluid from autosomal dominant polycystic kidneys.cyst infection in patients with autosomal-dominant polycystic kidney disease (adpkd) is often refractory to therapy, in part because of the limited entry of commonly used antibiotics into cyst fluid. to study the efficacy of trimethoprim-sulfamethoxazole in cyst infection, cyst fluid was obtained by percutaneous aspiration or at surgery from eight patients with adpkd receiving trimethoprim-sulfamethoxazole. cysts were categorized as nongradient or gradient by cyst-fluid sodium concentration. tri ...19873123761
resistance to quinolones.resistance to quinolones is only due to mutations. the mechanism and the range of quinolones involved, depend on the locus of the chromosomal mutation e.g. a mutation in the gyr a locus is associated with resistance due to changes in the gyrase. using high inocula in vitro, varying, but relatively low mutation rates of resistance to fluoroquinolones have been found (10(-6)-10(-12). in vitro transfers of bacterial strains in increasing concentrations of quinolones yield parallel increases of the ...19873125517
sterility testing of home and inpatient parenteral nutrition solutions.the microbial contamination rate was compared for parenteral nutrition solutions prepared by patients for home use and by pharmacy personnel for inpatient use. phase i validated the ivex 0.22-micron inline filter as a tool for microbiological testing by inoculating small numbers of organisms in 5% dextrose injection and testing for recovery. phase ii validated the same method for determining microbial contamination of total parenteral nutrition (tpn) solutions. phase iii compared inpatient and h ...19883125353
a shift from acute to chronic spontaneous pyelonephritis in male mm mice associated with a change in the causal micro-organisms.proteus mirabilis was the predominant cause of acute diabetes-associated pyelonephritis occurring spontaneously in male mm mice until they were segregated in a new environment. thereafter pasteurella pneumotropica and streptococcus faecalis emerged collectively as the most common causal organisms, the pyelonephritis became more chronic and proteus mirabilis isolates from faeces and urine produced atypical non-swarming colonies on blood agar plates. this did not account for the reduced pathogenic ...19883127633
[immunological activity of a proteus vaccine administered to volunteers simultaneously with a pyoimmunogen and adsorbed staphylococcal anatoxin].the comparative study of the immunological activity of proteus vaccine prepared from soluble antigenic complexes was made after the immunization of volunteers with this vaccine used in the form of a single preparation and in combination with pyoimmunogen (pseudomonas aeruginosa vaccine) and/or adsorbed staphylococcal toxoid. the injection of the vaccine in the form of a single preparation and in different combinations increased the ingestion of proteus cells by neutrophils. the injection of prot ...19873128030
antitumor effect of normal intestinal microflora on ehrlich ascites tumor.in order to investigate the antitumor activity of intestinal microflora, the constitution of normal flora was examined in humans, guinea pigs and mice. it was clarified that eubacterium, bifidobacterium and bacteroides were the predominant bacterial genera in humans. in addition, neither clostridium nor enterobacteriaceae was detected in guinea pigs and neither clostridium nor bifidobacterium was present in mice. total bacterial counts in tumor-bearing mice were reduced in comparison with those ...19883128498
induction of chromosomal beta-lactamases by different concentrations of clavulanic acid in combination with ticarcillin.the effect of different concentrations of clavulanic acid (ca) in combination with ticarcillin on beta-lactamase production and ticarcillin mic was studied in four clinical isolates of pseudomonas aeruginosa, enterobacter cloacae, serratia marcescens, citrobacter freundii and indole positive proteus strains. ticarcillin alone showed a low inducing effect for all species tested, ser. marcescens excepted. the increase in beta-lactamase activity after addition of ca (2-10 mg/l) was strain and speci ...19883128523
role of intestinal anaerobic bacteria in colonization resistance.the purpose of this study was to clarify the role of the intestinal anaerobe bacteria in colonization resistance. germfree mice were associated with escherichia coli c25 and either (a) no other species; (b) enterococcus; (c) escherichia coli m14 and proteus mirabilis, or (d) bacteroides fragilis and bacteroides vulgatus. all species colonized the cecum in high numbers, but only enterococcus significantly limited the translocation of escherichia coli c25 to mesenteric lymph nodes. however, the ov ...19883132368
[research on antibacterial and antifungal agents. vii. synthesis of (1-pyrryl)methylquinolines acids].some (1-pyrryl)methyl derivatives of 1-ethyl-1,4-dihydro-4-oxoquinoline-3-carboxylic acid were synthetized by the standard procedure involving gould-jacobs and lappin reactions. the above derivatives were tested microbiologically as nalidixic acid analogs and compared with some clinically useful 4-oxopyridine-3-carboxylic acids (nalidixic acid, pipemidic acid, norfloxacin, enoxacin and ciprofloxacin). their antibacterial activities were very weak.19873133240
activity of e-3846, a new fluoroquinolone, in vitro and in experimental cystitis and pyelonephritis in rats.the in vitro antibacterial activity of e-3846, a new fluoroquinolone carboxylic acid derivative with a pyrrol ring substituent at position 7, was evaluated in comparison with norfloxacin and ciprofloxacin. e-3846 was more active than the reference quinolones against staphylococcus species, including methicillin-resistant strains. e-3846 was similar to ciprofloxacin and more active than norfloxacin against streptococcus (enterococcus) faecalis. in general, e-3846 was more active than norfloxacin ...19883134844
a regional survey of the resistance to beta-lactam antibiotics in clinical isolates of (facultative) aerobic micro-organisms.from 1980 to 1986 a regional survey was performed to investigate the susceptibility of beta-lactam antibiotics in clinical isolates of escherichia coli, proteus mirabilis, klebsiella pneumoniae, enterobacter cloacae and pseudomonas aeruginosa in 6 hospitals located in the southern part of the netherlands. each year 300 to 400 strains were tested. the susceptibility to representatives of the older and the newer beta-lactam antibiotics was determined by a microbroth dilution assay using an inoculu ...19883135169
comparison of in vitro activity of cefotaxime and desacetylcefotaxime alone and in combination against 320 gram-negative clinical isolates.the antimicrobial activity of cefotaxime and its intermediate metabolite, desacetylcefotaxime, against 320 gram-negative bacterial strains was analysed to investigate whether combination of the 2 substances led to increased bactericidal activity. the in vitro study of the minimum inhibitory concentration (mic) showed the combination to be more effective against escherichia coli, klebsiella spp., enterobacter spp. and proteus mirabilis, requiring less than or equal to 50% of the concentration of ...19883135171
antibiotic prophylaxis for surgery after vesico-ureteral reflux in children. 19883135164
phosphinic acid inhibitors of d-alanyl-d-alanine ligase.we report the synthesis of a series of phosphinic acid dipeptide analogues, nh2ch(r1)po(oh)ch2ch(r2)co2h, related to dala-dala. the best of these compounds are potent, essentially irreversible inhibitors of dala-dala ligase, and their preferred stereochemistry was shown by chiral synthesis of (1(s)-aminoethyl)(2(r)-carboxy-1-n-propyl)phosphinic acid, 12b, and by x-ray crystallography of its derivative benzyl [1(s)-[(benzyloxycarbonyl)-amino]ethyl](2(r)-carbomethoxy-1-propyl) phosphinate, 13, to ...19883137344
further diagnostic thoughts about the elephant man.further evidence for a diagnosis of the elephant man's condition is reviewed. it is known that the elephant man had "mocassin" lesions, hyperostoses of the skull, and absence of café-au-lait spots, all of which are characteristic of proteus syndrome. recently, questions have been raised about his skeletal findings and their relevance to neurofibromatosis. however, other skeletal diagnoses have been entertained, including maffucci syndrome, paget's disease of bone, pyarthrosis, and fibrous dyspla ...19883135754
[demonstration of glycocalyx formation in vitro in citrobacter freundii, proteus mirabilis and planococcus sp.: the effect of polyosidases].three bacterial species of different origin, citrobacter freundii, proteus mirabilis and planococcus sp., formed glycocalyx in vitro and thereby showed that the phenomena does not only occur in vivo contrary to the opinion of many authors. the glycocalyx was produced in the attachment processes used or both extra- and intercellular attachment. the aggregates formed caused large errors in bacterial counts and led to questioning of the very principle of the counting procedures. the glycocalyx was ...19873135828
[evolution from 1976 to 1986 of bacterial prevalence and sensitivity to urinary antibiotics of bacteria isolated from urine cultures at a city microbiology laboratory].the evolution of the prevalence of the bacterial strains isolated from uti cases (bacteriuria greater than or equal to 10(5)/ml has been studied over a 11 year-period from 1976 to 1986, in a clinical practice microbiological laboratory from a rural area. e. coli was the overall most frequently isolated species, followed by p. mirabilis (74.4% and 11.2% respectively), by the klebsiella-enterobacter group (4.8%), and staphylococci (represented by coagulase negative staphylococci) (4.9%). the relat ...19883136425
[chemical and immunochemical characteristics of antigenic preparations from staphylococcus aureus and klebsiella pneumoniae as the components for an associated vaccine].s. aureus aqueous extract and k. pneumoniae hydroxylamine vaccine were studied by means of chemical and immunochemical analytical techniques. the preparations were found to contain, respectively, 7.0% nad 53.5% of neutral monosaccharides, 6.5% and 0.7% of nucleic acids, as well as protein in approximately equal amounts (11.63-14.0%). in experiment of immunodiffusion, immunoelectrophoresis and rocket immunoelectrophoresis in homologous systems with hyperimmune antimicrobial sera the preparations ...19883140544
nitrosamine formation by denitrifying and non-denitrifying bacteria: implication of nitrite reductase and nitrate reductase in nitrosation catalysis.biochemical, microbiological and genetic studies were done to characterize the mechanism of bacterial formation of n-nitrosomorpholine (nmor) from morpholine and nitrite at neutral ph. in escherichia coli and proteus morganii, the nitrosating activity was markedly induced when bacteria were cultured under anaerobiosis in minimal medium containing nitrate, while in the presence of nitrite there was no induction. however, induction of the nitrosating activity in pseudomonas aeruginosa occurred in ...19883141563
Displaying items 3101 - 3200 of 15180