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evaluation of a modified double-disc synergy test for detection of extended spectrum beta-lactamases in ampc beta-lactamase-producing proteus mirabilis.the detection of extended-spectrum beta-lactamases (esbls) in gram-negative bacteria that produce ampc beta-lactamases is problematic. in the present study, the performance of modified double-disc synergy test (mddst) that employs a combination of cefepime and piperacillin-tazobactam for the detection of proteus mirabilis producing extended spectrum and ampc beta-lactamases was evaluated and compared with double-disc synergy test (ddst) and nccls phenotypic disc confirmatory test (nccls-pdct). a ...200818227600
[an improved model of a catheterised human bladder for screening bactericidal agents].catheter encrustation and associated blockage by a crystalline proteus mirabilis biofilm constitute a continuous problem in long-term catheterised patients. the objective of the present work was to verify a new, physiological bladder model possessing the ability to show that triclosan-blocking solutions exert bactericidal and bacteriostatic activities.200818228188
efficacy of ertapenem for consolidation therapy of extended-spectrum beta-lactamase-producing gram-negative infections: a case series report.infections caused by extended-spectrum beta-lactamase (esbl)-producing gram-negative organisms are becoming increasingly common and present significant challenges in terms of treatment. carbapenems is the antibiotic class of choice for treatment of these types of infections. ertapenem is the newest carbapenem, capable of being dosed once daily, and has some in vitro but little in vivo evidence supporting its use for the treatment of these resistant infections.200818230703
diabetic foot osteomyelitis.bone infection in the diabetic foot is always a complication of a preexisting infected foot wound. prevalence can be as high as 66%. diagnosis can be suspected in two mains conditions: no healing (or no depth decrease) in spite of appropriate care and off-loading, and/or a visible or palpated bone with a metal probe. the first recommended diagnostic step is to perform (and if necessary to repeat) plain radiographs. after a four-week treatment period, if plain radiographs are still normal, suspic ...200818242114
antibacterial and antifungal activities of different parts of tribulus terrestris l. growing in iraq.antimicrobial activity of organic and aqueous extracts from fruits, leaves and roots of tribulus terrestris l., an iraqi medicinal plant used as urinary anti-infective in folk medicine, was examined against 11 species of pathogenic and non-pathogenic microorganisms: staphylococcus aureus, bacillus subtilis, bacillus cereus, corynebacterium diphtheriae, escherichia coli, proteus vulgaris, serratia marcescens, salmonella typhimurium, klebsiella pneumoniae, pseudomonas aeruginosa and candida albica ...200818257138
evaluation of pyelonephritis in culled indoor and outdoor high parity sows.the present trial was conducted in hungary in neighboring large indoor and outdoor pig production units, belonging to the same breeding company. rejected kidneys from 201 (out of 241; 83.4%) outdoor, and 191 (out of 512, 37.3%) indoor high parity sows, with previous history of recidiving postparturient fever and excessive postparturient vulvovaginal discharge were gross pathologically bacteriologically, and histologically evaluated. all rejected kidneys revealed chronic pyelonephritis. in outdoo ...200818265757
a rare case of proteus syndrome.we report a rare case of proteus syndrome which presented with dysphagia due to unilateral tonsillar hypertrophy.200818279570
isolated plantar collagenoma not associated with proteus syndrome.isolated plantar cerebriform collagenomas are a relatively rare type of connective tissue nevus. they have been suggested to be pathognomonic of proteus syndrome. however, their presence is now considered to be a major criterion of proteus syndrome, but the diagnosis of proteus syndrome also requires the presence of other minor criteria. we present an unusual case of an acquired plantar cerebriform collagenoma, which is not associated with proteus syndrome. collagenomas, or connective tissue nev ...200818280350
antimicrobial activity of the crude extracts and five flavonoids from the twigs of dorstenia barteri (moraceae).the aim of this study was to evaluate the antimicrobial activity of the crude extract of the twigs of dorstenia barteri (dbt) as well as that of four of the five flavonoids isolated from this extract. gram-positive bacteria (six species), gram-negative bacteria (12 species) and fungi (four species) were used. the agar disc diffusion test was used to determine the sensitivity of the tested samples while the well micro-dilution was used to determine the minimal inhibition concentrations (mic) and ...200818280679
cache write generate for parallel image processing on shared memory architectures.we investigate cache write generate, our cache mode invention. we demonstrate that for parallel image processing applications, the new mode improves main memory bandwidth, cpu efficiency, cache hits, and cache latency. we use register level simulations validated by the uw-proteus system. many memory, cache, and processor configurations are evaluated.199618285208
performance of chromid esbl, a chromogenic medium for detection of enterobacteriaceae producing extended-spectrum beta-lactamases.the chromogenic agar medium chromid esbl (biomérieux) was compared with blse agar medium (aes) for selective isolation and presumptive identification of extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae from clinical samples. a total of 765 samples (468 rectal swabs, 255 urine samples and 42 pulmonary aspirations) obtained from 547 patients was processed. all bacterial strains isolated on either medium were further characterized using biochemical tests, and esbl producers were ...200818287293
isolation, identification and application of novel bacterial consortium tj-1 for the decolourization of structurally different azo dyes.a novel bacterial consortium (tj-1), which could decolorize acid orange 7 (ao7) and manyother azo dyes, was developed. in tj-1 three bacterial strains were identified as aeromonas caviae, proteus mirabilis and rhodococcus globerulus by 16s rrna gene sequence analysis. ao7 decolorization was significantly higher with the use of consortium as compared to the use of individual strains, indicating complementary interactions among these strains. ao7 decolorization was observed under microaerophilic c ...200818289845
increase in beta-lactam-resistant proteus mirabilis strains due to ctx-m- and cmy-type as well as new veb- and inhibitor-resistant tem-type beta-lactamases.the aim of this study was to characterize the different inhibitor-resistant tem beta-lactamases, extended-spectrum beta-lactamases (esbls) and plasmid-mediated ampc beta-lactamases implicated in beta-lactam resistance in proteus mirabilis, which has increased over recent years.200818292096
[synthesis and antimicrobial activity of some new (sulfon-amidophenyl)-amide derivatives of n-(m-nitrobenzoyl)-d,l-methionine].in order to obtain new compounds with potential antimicrobial activity some new (sulfon-amidophenyl)-amide derivatives of n-(m-nitrobenzoil)-d,l-methionine have been synthesized. the compounds have been obtained by decyclization reaction of 2-(m-nitrophenyl)-4-(beta-p-methylthioethyl)- delta2-5-oxazolinone with various sulfonamides. the chemical structure of the new sulfonamides was confirmed by elemental analysis data and ir and 1nmr spectral measurements. the compounds were also investigated f ...200718293716
surveillance for plasmid-mediated quinolone resistance determinants in enterobacteriaceae within the calgary health region, canada: the emergence of aac(6')-ib-cr.a study was designed to determine the prevalence of plasmid-mediated quinolone resistance (pmqr) determinants among clinical isolates of enterobacteriaceae from the calgary health region (chr).200818296438
viper metalloproteinase (agkistrodon halys pallas) with antimicrobial activity against multi-drug resistant human pathogens.metalloproteinases are abundant enzymes in crotalidae and viperidae snake venoms. snake venom metalloproteinases (svmps) comprise a family of zinc-dependent enzymes, which display many different biological activities. a 23.1 kda protein was isolated from agkistrodon halys (pallas, chinese viper) snake venom. the toxin is a single chain polypeptide with a molecular weight of 23146.61 and an n-terminal sequence (miqvllvticlavfpyqgssiiles) relatively similar to that of other metalloprotein-like pro ...200818297685
synthesis, structure and biological activities of cobalt(ii) and zinc(ii) coordination compounds with 2-benzimidazole derivatives.in this work we present the synthesis, structural and spectroscopic characterisation of a series of cobalt(ii) and zinc(ii) coordination compounds with benzimidazole (bz) and its 2-benzimidazole derivatives: 2-aminobenzimidazole (2ab), albendazole (abz) and tris(2-benzimidazolylmethyl)amine (ntb). the compounds were evaluated for their in vitro antimicrobial activity against staphylococcus aureus, micrococcus luteus, salmonella typhi, pseudomonas aeruginosa, escherichia coli and proteus vulgaris ...200818304644
case 131: proteus syndrome. 200818309020
experimental and qsar studies on antimicrobial activity of benzimidazole derivatives.twenty eight analogues of benzimidazoles had been synthesized and tested for their antimicrobial activity against four bacteria (staphylococcus auerus, escherichia coli, bacillus pumilus and proteus vulgaris) and two fungi (aspergillus flavus and aspergilus niger). compounds with r as c6h4no2 and r' as so2c6h4-ch3(p), with r as c6h4och3 and r' as so2c6h4-ch3(p), and with r as ch2c6h5 and r' as ch2(ch2)9cl exhibited comparable or higher antibacterial activity than ciprofloxacin against s. auerus ...200818310935
identification of an inhibitor of the murc enzyme, which catalyzes an essential step in the peptidoglycan precursor synthesis pathway.the pathway for synthesis of the peptidoglycan precursor udp-n-acetylmuramyl pentapeptide is essential in gram-positive and gram-negative bacteria. this pathway has been exploited in the recent past to identify potential new antibiotics as inhibitors of one or more of the mur enzymes. in the present study, a high-throughput screen was employed to identify potential inhibitors of the escherichia coli murc (udp-n-acetylmuramic acid:l-alanine ligase), the first of four paralogous amino acid-adding ...200818315498
role of rppa in the regulation of polymyxin b susceptibility, swarming, and virulence factor expression in proteus mirabilis.proteus mirabilis, a human pathogen that frequently causes urinary tract infections, is intrinsically highly resistant to cationic antimicrobial peptides, such as polymyxin b (pb). to explore the mechanisms underlying p. mirabilis resistance to pb, a mutant which displayed increased (> 160-fold) sensitivity to pb was identified by transposon mutagenesis. this mutant was found to have tn5 inserted into a novel gene, rppa. sequence analysis indicated that rppa may encode a response regulator of th ...200818316383
[new perspectives of the phage therapy].a solution to the problem of the increasing number of antibiotic-resistant bacterial strains can be the use of rational phage therapy. in the past, bacteriophages (phages) were often used for the treatment and prevention of infections and unlike antibiotic therapy, phage therapy caused almost no serious side effects. while previously several preparations containing whole phage particles were available for phage therapy, currently, the isolation of well characterised and purified phage components ...200718320502
antimicrobial resistance patterns of clinical bacterial isolates in southwestern ethiopia.the aim of this study was to assess the pattern of bacterial resistance to antimicrobial agents in a hospital setting in southwest ethiopia.200718326346
[nosocomial urinary tract infections in the geriatric hospital - pathogen spectrum and resistancies].we examined 834 isolates of urinary cultures (26 fungi and 808 isolates from 28 bacteria) in an academic geriatric teaching hospital for microbial spectrum and resistance analysis. of the bacteria, 90% were associated to ten species. detection rate of mrsa and esbl was 1%, detection rate for pseudomonas aeruginosa, proteus species, coagulase negative staphylococci and klebsiella species were higher than in an university clinic (p < 0.001). resistency rates were high for the 10 most frequent bact ...200918327687
comparative activity of meropenem in us medical centers (2007): initiating the 2nd decade of mystic program surveillance.since 1997, the meropenem yearly susceptibility test information collection (mystic) program has monitored the antimicrobial activity of broad-spectrum agents against pathogens from hospitalized patients. in the united states, 2894 isolates were submitted in 2007 from 15 sites, including 1392 enterobacteriaceae, 643 nonfermentative gram-negative bacilli, and 829 gram-positive cocci. all isolates were tested by broth microdilution methods. meropenem (mic(90) range, 0.12-2 microg/ml) exhibited the ...200818329835
[etiology of urinary tract infections and antimicrobial susceptibility of urinary pathogens].with the objective of knowing the common etiological agents in urinary infection and comparing its antimicrobial susceptibility in nosocomial and community-acquired urinary infections, we analyse all the urine bacteriological exams from the serviço de patologia clínica do centro hospitalar do nordeste, epe - unidade hospitalar de bragança, during a two years period (april 2004 to march 2006). during this period, 4018 urine bacteriological exams were made. the cultural exam was positive in 572 sa ...200818331698
[classical kaposi's sarcoma in a 78-year-old woman]. 200818338143
structure, self-assembly, and dual role of a beta-defensin-like peptide from the chinese soft-shelled turtle eggshell matrix.biomineral matrix formation and molecular recognition are two important processes associated with eggshell biomineralization. to understand these two processes, a major intracrystalline peptide, pelovaterin, was isolated from turtle (pelodiscus sinensis) eggshell and its tertiary and quaternary structures were established. the global fold of pelovaterin is similar to that of human beta-defensins but has a large hydrophobic core and a short hydrophilic n-terminal segment, which is not preserved i ...200818341335
antimicrobial resistance of bacterial isolates from respiratory care wards in taiwan: a horizontal surveillance study.a horizontal surveillance study was conducted to identify common bacteria and mycobacteria from 611 respiratory aspirates and 165 urinary samples from 611 patients hospitalised at 17 respiratory care wards (rcws) in taiwan. some major resistance phenotypes, including methicillin-resistant staphylococcus aureus (mrsa), extended-spectrum beta-lactamase (esbl)-producing escherichia coli, klebsiella pneumoniae and proteus mirabilis, and multidrug-resistant pseudomonas aeruginosa (mdr-pa) and acineto ...200818358701
pharmacodynamic performance of tigecycline versus common intravenous antibiotics for the empiric treatment of complicated skin and skin structure infections.in clinical trials tigecycline was similar to vancomycin/aztreonam for complicated skin and skin structure infections, but data comparing it with other widely used antibiotics are lacking. we predicted the pharmacodynamic cumulative fraction of response (cfr) of tigecycline against bacteria implicated in complicated skin and skin structure infections and compared it with the cfrs for piperacillin/tazobactam, ceftriaxone, levofloxacin, and imipenem/cilastatin.200818363469
some microbiological aspects of rats captured alive at the port city of piraeus, greece.the aim was to investigate the presence of leptospira infection and the antibiotic resistance of enterobacteriaceae among wild rats living in a densely populated area. for this purpose 3-4 large traps were placed daily across the commercial port-front of piraeus for the duration of two months. the rats captured were transferred in the laboratory alive, where samples were freshly collected. none of the 25 rats captured was found to be infected with leptospira. only one rat was infected with salmo ...200818365804
application of capillary zone electrophoresis (cze) to the determination of pathogenic bacteria for medical diagnosis.microorganisms like bacteria, viruses, fungi, etc. have a major impact on human, animal, and plant life. several decades ago it was observed that intact cells suspended in an isotonic salt solution moved toward the anode and the mobility was proportional to the density of the charge located on the cell surfaces. since then, efforts have been made to make electrophoresis a useful tool in cell studies. microorganisms are no exception. in the present work we applied the capillary zone electrophores ...200818373084
application of two different kinds of sera against the proteus penneri lipopolysaccharide core region in search of epitopes determining cross-reactions with antibodies.proteus penneri lipopolysaccharide (lps) core regions are characterized by a greater structural variability than that observed in other enterobacteriaceae. this fact and the small amount of published data concerning the serological activity of this part of p. penneri lps prompted an examination of which fragment might determine cross-reactions with antibodies. to date, such epitopes have been found in the lps core regions of p. mirabilis and p. vulgaris strains.200818373243
complete genome sequence of uropathogenic proteus mirabilis, a master of both adherence and motility.the gram-negative enteric bacterium proteus mirabilis is a frequent cause of urinary tract infections in individuals with long-term indwelling catheters or with complicated urinary tracts (e.g., due to spinal cord injury or anatomic abnormality). p. mirabilis bacteriuria may lead to acute pyelonephritis, fever, and bacteremia. most notoriously, this pathogen uses urease to catalyze the formation of kidney and bladder stones or to encrust or obstruct indwelling urinary catheters. here we report t ...200818375554
cerebriform plantar hyperplasia: the major cutaneous feature of proteus syndrome. 200818377602
antimicrobial activity of 2,5-dihydroxy-3-methyl-1,4-benzoquinone from embelia schimperi.chromatographic separation of an ethyl acetate extract from embelia schimperi led to the isolation of a new compound identified as 2,5-dihydroxy-3-methyl-1,4-benzoquinone (1) on the basis of spectroscopic and physical data. the plant's crude extract and pure compound 1 were assayed for in vitro antimicrobial activity against clinical strains of salmonella spp., proteus spp., pseudomonas aeruginosa, klebsiella pneumoniae, escherichia coli, cryptococcus neoformans, shigella dysentriae and staphylo ...200818386487
crossed fused renal ectopia with calculi. 200818387483
the establishment of resistance phenotypes for bacteria isolated from outpatients in urine cultures.from 1911 outpatients, who addressed a timişoara private clinical laboratory, from january to december 2005, we collected 1,889 urine cultures, 431 being positive. bacteria identification was generally done using morphological, cultural, biochemical characters and pathogenicity tests. sensitivity testing to antimicrobial medical drugs was done by using the classical diffusion kirby-bauer method and the automatic analyzer osiris, also. the main bacteria involved in the etiology of these infection ...200618389723
managing chronic otitis media with cholesteatoma report of 223 patients seen in a 5-year period.to assess the clinical features, diagnosis, and treatment of patients having chronic otitis media (com) with cholesteatoma seen in the ent/head and neck surgery clinic at the university clinical center, prishtina, kosovo.200818390126
declination of copper toxicity in pigeon pea and soil system by growth-promoting proteus vulgaris knp3 strain.the copper-resistant (1318 microm cuso(4).5h(2)o) strain knp3 of proteus vulgaris was isolated from soil near the panki power plant, kanpur, india, and was used to inoculate pigeon pea (cajanus cajan var. ups-120) seeds grown in soil for 60 days in the presence of 600 microm cuso(4).5h(2)o. a study of siderophore production (126.34 +/- 0.52 microg ml(-1)) and its subsequent effects on plant growth promotion under in situ conditions was conducted. the parameters that were monitored included the p ...200818392891
kinetic study approach of remazol black-b use for the development of two-stage anoxic-oxic reactor for decolorization/biodegradation of azo dyes by activated bacterial consortium.the laboratory-isolated strains pseudomonas aeruginosa, rhodobacter sphaeroides, proteus mirabilis, bacillus circulance, nad 1 and nad 6 were observed to be predominant in the bacterial consortium responsible for effective decolorization of the azo dyes. the kinetic characteristics of azo dye decolorization by bacterial consortium were determined quantitatively using reactive vinyl sulfonated diazo dye, remazol black-b (rb-b) as a model substrate. effects of substrate (rb-b) concentration as wel ...200818394798
[frequency and antibiotic susceptibility of bacteria identified in urine].the authors had for aim to evaluate the frequency of isolation and the antibiotic susceptibility of bacteria responsible for urinary infections among in and out-patients.200818395378
epidermal nevus syndromes.the term "epidermal nevus syndrome" (ens) has been used to describe the association of epidermal hamartomas and extra-cutaneous abnormalities. epidermal nevi follow the lines of blaschko. the majority of the extra-cutaneous manifestations involve the brain, eye, and skeletal systems. several subsets with characteristic features have been delineated including the nevus sebaceous syndrome, proteus syndrome, child syndrome, becker nevus syndrome, nevus comedonicus syndrome, and phakomatosis pigment ...200718395670
synthesis, characterization and electrochemistry of 4'-functionalized 2,2':6',2''-terpyridine ruthenium(ii) complexes and their biological activity.the synthesis and characterization of ru(ii) terpyridine complexes derived from 4'-functionalized 2,2':6',2''-terpyridine ligands by a multi step procedure have been described. the complexes are redox-active, showing both metal-centred (oxidation) and ligand-centred (reduction) processes. the antibacterial and antifungal activity of the synthesized ruthenium(ii) complexes [ru(attpy)2](pf6)2 (attpy = 4'-(4-acryloyloxymethylphenyl)-2,2':6',2''-terpyridine); [ru(mttpy)2](pf6)2 (mttpy = 4'-(4-methac ...200818398539
anti-tumor preventative effect of mono-therapy with the use of proteus vaccine, staphylococcus antitoxin and divaccine of staphylococcus-proteus.anti-tumor preventative effect of mono-therapy with the use of proteus vaccine, staphylococcus antitoxin and divaccine of staphylococcus-proteus has been studied. experiments were carried out on 80 non-purebred laboratory white mice (age--3-3,5 months, body mass--18-20 g) and 60 rats (body mass--100-120 g) using intraperitoneal inoculation of ehrlich's adenocarcinoma (ascitic form--eat, in mice, cancer cells--3 x 10(6)), and subcutaneous inoculation of sarcoma s-45 (in rats). anti-tumor preventa ...200818401057
antibacterial activity of some medicinal plant extracts.antibacterial activity of hot aqueous and methanolic extracts prepared from six plants (terminallia chebula, terminallia bellerica, phyllanthus emblica, punica granatum, lawsonia alba and mikania micrantha) used in traditional folk medicines of india were screened against five pathogenic bacteria (staphylococcus aureus mtcc 2940, bacillus subtilis mtcc 441, escherichia coli mtcc 739, proteus vulgaris mtcc 426 and enterobacter aerogenes mtcc 111). the highest antibacterial potentiality was exhibi ...200818404337
limited functional conservation of a global regulator among related bacterial genera: lrp in escherichia, proteus and vibrio.bacterial genome sequences are being determined rapidly, but few species are physiologically well characterized. predicting regulation from genome sequences usually involves extrapolation from better-studied bacteria, using the hypothesis that a conserved regulator, conserved target gene, and predicted regulator-binding site in the target promoter imply conserved regulation between the two species. however many compared organisms are ecologically and physiologically diverse, and the limits of ex ...200818405378
the in-vitro antimicrobial activity of abrus precatorius (l) fabaceae extract on some clinical pathogens.to investigate the in-vitro antimicrobial activities of the extracts of abrus precatorius on some clinical isolates as resistance to available and affordable antibiotics by these pathogens is on the increase.200818408781
some observations on the migration of proteus mirabilis and other urinary tract pathogens over foley catheters.the ability of uropathogens to migrate along external surfaces of silicone foley catheters was examined. proteus mirabilis and pseudomonas aeruginosa were the most motile organisms. p. aeruginosa migrated over both triclosan-impregnated and nitrofurazone-impregnated catheters, but these antibacterials inhibited the migration of p. mirabilis.200818419368
[etiology of community-acquired lower urinary infections and antimicrobial resistance of escherichia coli: a national surveillance study].the increasing rates of resistance exhibited by uropathogens represent a serious problem. the aim of this study was to determine, in spain, the etiology of community-acquired lower urinary infections and antimicrobial resistance of escherichia coli isolates.200818423165
[community-acquired low urinary tract infection]. 200818423168
a novel autotransporter of uropathogenic proteus mirabilis is both a cytotoxin and an agglutinin.one of the six predicted proteus mirabilis autotransporters (ats), orf c2341, is predicted to contain a serine protease motif and was earlier identified as an immunogenic outer membrane protein in p. mirabilis. the 3.2 kb gene encodes a 117 kda protein with a 58-amino-acid-long signal peptide, a 75-kda-long n-terminal passenger domain and a 30-kda-long c-terminal translocator. affinity-purified 110 kda at exhibited chymotrypsin-like activity and hydrolysed n-suc-ala-ala-pro-phe-pna and n-suc-ala ...200818430084
vascular malformation and hemangiomatosis syndromes: spectrum of imaging manifestations.objective: the purpose of this review is to describe the role of imaging and associated findings in the diagnosis of blue rubber bleb nevus syndrome, proteus syndrome, klippel-trénaunay syndrome, and kasabach-merritt syndrome. conclusion: blue rubber bleb nevus, proteus, klippel-trénaunay, and kasabach-merritt syndromes are a diverse group of vascular malformation and hemangiomatosis syndromes. both cutaneous and visceral vascular lesions are associated with these disorders. accurate diagnosis o ...200818430846
rapamycin treatment for a child with germline pten mutation.a 9-month-old boy with proteus syndrome and a de novo germline mutation in the tumor suppressor pten was referred to a specialist centre for management. over the first years of life, the patient developed life-threatening respiratory dysfunction and malnutrition because of progressive growth of hamartomas affecting the chest, mediastinum, abdomen and pelvis.200818431376
selective antimicrobial activity of maggots against pathogenic bacteria.maggot therapy, also known as biosurgery, is an ancient method for the healing of chronic infected wounds. although clinicians have reported on the beneficial activities of the lucilia sericata larvae that have been used for healing chronic wounds, the selectivity of this therapy against the different pathogenic micro-organisms that are found in chronic wounds has never been analysed. in the present study, we have investigated the in vitro activities of larval excreta/secreta both against select ...200818436596
in vitro interaction between mecillinam and piperacillin-tazobactam in the presence of azithromycin against members of the enterobacteriaceae family and pseudomonas aeruginosa.mecillinam was tested in vitro alone or in combination with piperacillin-tazobactam and azithromycin against representative species of the enterobacteriaceae family and pseudomonas aeruginosa to extend its antibacterial spectrum, and to protect mecillinam from inactivating enzymes taking advantage of the presence of tazobactam. drug interactions were studied by microdilution method, by selection of spontaneous resistant mutants on agar plates containing the drugs in combination and by time kill ...200818437840
soft tissue infections with arcanobacterium haemolyticum: report of three cases.we report here three polymicrobial wound infections associated with arcanobacterium haemolyticum in rural patients aged between 60-65 years. in two patients, one with cellulitis and the other with postoperative wound infection following amputation of the limb, arcanobacterium haemolyticum was isolated repeatedly along with beta haemolytic streptococci (bhs). the bhs belonged to lancefield's group g and group c respectively. in another patient, who was a diabetic with chronic osteomyelitis, arcan ...200818445965
secondary chromosomal attachment site and tandem integration of the mobilizable salmonella genomic island 1.the salmonella genomic island 1 is an integrative mobilizable element (ime) originally identified in epidemic multidrug-resistant salmonella enterica serovar typhimurium (s. typhimurium) dt104. sgi1 contains a complex integron, which confers various multidrug resistance phenotypes due to its genetic plasticity. previous studies have shown that sgi1 integrates site-specifically into the s. enterica, escherichia coli, or proteus mirabilis chromosome at the 3' end of thdf gene (attb site).200818446190
[pleural empyema secondary to pyonephrosis]. 200818448021
identification of a novel storage glycine-rich peptide from guava (psidium guajava) seeds with activity against gram-negative bacteria.bacterial pathogens cause an expressive negative impact worldwide on human health, with ever increasing treatment costs. a significant rise in resistance to commercial antibiotics has been observed in pathogenic bacteria responsible for urinary and gastro-intestinal infections. towards the development of novel approaches to control such common infections, a number of defense peptides with antibacterial activities have been characterized. in this report, the peptide pg-amp1 was isolated from guav ...200818448201
comparative study of electrokinetic potentials and binding affinity of lipopolysaccharides-chitosan complexes.electrokinetic properties of complexes of chitosan (ch) with lipopolysaccharides (lpss) from escherichia coli o55:b5, yersinia pseudotuberculosis 1b 598, and proteus vulgaris o25 (48/57) and their size distribution were investigated using zeta-potential distribution assay and quasi-elastic light scattering. the interaction of lps from different microorganisms with chitosan at the same w/w ratio of components (1:1) resulted in the formation of complexes in which the negative charge of lps was neu ...200818455288
identification of plasmid-encoded extended spectrum beta-lactamases produced by a clinical strain of proteus mirabilis.emergence and dissemination of multiresistant strain of proteus mirabilis have made infections treatment more difficult that this bacterium is responsible. the aim of this study is to determine the implication of the enzymatic mechanism and to describe the properties of esbls (extended spectrum beta-lactamases). a clinical strain of proteus mirabilis sm514 isolated in the intensive care unit at the military hospital in tunisia during the period 2004 was found to be highly resistant to cephalospo ...200918456422
microbial degradation of palm (elaeis guineensis) biodiesel.the kinetics of biodegradation of palm-derived fatty methyl and ethyl esters (elaeis guineensis biodiesel) by a wild-type aerobic bacterial population was measured at 20 degrees c, as the rate of oxygen uptake by a manometric technique. the methyl and ethyl biodiesels were obtained by potassium-hydroxide catalysed trans-esterification of palm oil, respectively. the bacterial flora included the genera bacillus, proteus, pseudomonas, citrobacter and enterobacter. the rate of oxygen uptake for palm ...200618457175
molecular survey of beta-lactamases conferring resistance to newer beta-lactams in enterobacteriaceae isolates from polish hospitals.the first national survey of resistance to newer beta-lactams in nosocomial populations of enterobacteriaceae in poland was performed. the study covered all nonrepetitive enterobacterial isolates cultured from specimens from inpatients in 13 regional secondary-care hospitals from november 2003 to january 2004. among 2,388 isolates, the predominant species was escherichia coli (59.6%), followed by proteus mirabilis (14.5%) and klebsiella spp. (8.5%). the frequency of extended-spectrum beta-lactam ...200818458126
traps and attractants for wood-boring insects in ponderosa pine stands in the black hills, south dakota.recent large-scale wildfires have increased populations of wood-boring insects in the black hills of south dakota. because little is known about possible impacts of wood-boring insects in the black hills, land managers are interested in developing monitoring techniques such as flight trapping with semiochemical baits. two trap designs and four semiochemical attractants were tested in a recently burned ponderosa pine, pinus ponderosa dougl. ex laws., forest in the black hills. modified panel and ...200818459406
[quinolone resistance in gram negative rods in iceland and association with antibiotic use].fluoroquinolones are bacteriocidal drugs that are widely used to treat severe urinary and respiratory tract infections. studies show that resistance to fluoroquinolones is continuously increasing both in europe and the united states. the purpose of this study was to measure the frequency of fluoroquinolone resistance in the most prevalent gram negative rods and look at the correlation with fluoroquinolone use over the last 8 years.200818460726
effects of rifaximin on bacterial virulence mechanisms at supra- and sub-inhibitory concentrations.rifaximin, a poorly absorbed rifamycin derivative, exhibited time-dependent bactericidal activity and at concentrations as low as 1/32 of the minimum inhibitory concentration (mic) caused morphological alterations in both susceptible and resistant bacterial strains. spontaneous rifaximin-resistant clones appeared with an incidence of 2.6 x 10(-7). the percentage of escherichia coli cells cured of various plasmids ranged from: 4.5-70% (flac), 0-18% (pbp507), 7.7-43.8% (plasmid carrying esbl genes ...200818467244
purification, characterization and bactericidal activities of basic phospholipase a2 from the venom of agkistrodon halys (chinese pallas).agkistrodon snake venoms contain a variety of phospholipases (pla2), some of which are myotoxic. in this study, we used reverse-phase hplc to purify pla2 from the venom of agkistrodon halys. the enzyme named as agktx-ii, a basic asp49 pla2, has a molecular masses of 13,869.05. the amino acid sequence and molecular mass of agktx-ii was identical to those of an asp49 basic myotoxic pla2 previously isolated from this venom. antibacterial activities were tested by susceptibility and broth-dilution a ...200818472013
proteus mirabilis in nonpuerperal breast infection: a nosocomial infection? 199518472895
synthesis, characterization and antimicrobial activity of d metal complexes of some 2-substituted-1h-benzimidazoles.the metal complexes of nine 2-substituted-1h-benzimidazoles (i-ix) with ni(ii), pd(ii), cu(ii), ag(i), zn(ii) salts were synthesized. the compounds were characterized by melting point, analytical data, ir spectroscopy and magnetic susceptibility. the antimicrobial activity of the compounds was determined by the disk diffusion method in mueller-hinton agar on staphylococcus aureusatcc 6538, staphylococcus epidermidis atcc 12228, escherichia coli atcc 8739, klebsiella pneumoniae atcc 4352, pseudom ...199918472907
bsac standardized disc susceptibility testing method (version 7).the changes that have been made to the previous version of the recommendations (version 6) are as follows: medium and incubation condition for testing acinetobacter spp. (tables 1 and 6); use of cefoxitin as an indicator antibiotic for detecting methicillin/oxacillin/cefoxitin resistance in coagulase-negative staphylococci (tables 1, 6 and 11); mic breakpoint for co-trimoxazole based on the trimethoprim concentration in a 1:19 combination with sulfamethoxazole (tables 7, 10, 11, 12, 15, 16 and 1 ...200818474513
vaginal mucositis in measles.measles (rubeola), a common childhood exanthema, occurs infrequently in adults. vaginal mucositis in association with measles is not commonly described.199518475409
structural and biological behaviour of co(ii), cu(ii) and ni(ii) metal complexes of some amino acid derived schiff-bases.biologically active tridentate amino acid (alanine, glycine & tyrosine) derived schiff-bases and their co(ii), cu(ii) & ni(ii) complexes have been synthesised and characterised on the basis of their conductance and magnetic measurements, elemental analysis and (13)c-nmr, (1)h-nmr, ir and electronic spectral data. these schiff-bases and their complexes have been evaluated for their antibacterial activity against bacterial species such as staphylococcus aureus, escherichia coli, klebsiella pneumon ...199718475798
synthesis, characterization and role of anions (nitrate, sulphate, oxalate and acetate) \in the biological activity of hydrazine derived compounds and their metal chelates.hydrazine derived compounds and its co(ii), cu(ii) and ni(ii) chelates having the same metal ion but different anions (e.g., nitrate, sulphate, oxalate and acetate) have been synthesised and characterised by their physical, spectral and analytical data. in order to evaluate the participating role of anions in the antibacterial activity, these synthesised ligands and its metal chelates have been screened against bacterial species such as staphylococcus aureous, pseudomonas aeruginosa, klebsiella ...199718475813
structure-activity relationships for some diamine, triamine and schiff base derivatives and their copper(ii) complexes.ethylenediamine (en), putrescine (pu), diethylenetriamine (dien), dipropylenetriamine (dpta), spermidine (spmd) and their cu(ii) compounds as well as the schiff bases with 2-furaldehyde (dienoo), 2- thiophenecarboxaldehyde (dienss) and pyrrole-2-carboxaldehyde (diennn) of dien and that of dpta with 2- thiophenecarboxaldehyde (dptass), were prepared and characterised. they were tested against bacillus substilis, bacillus cereus, staphylococcus aureus, escherichia coli, proteus vulgaris and xantho ...199818475868
c-3 substituted lawsonemonoximates of holmium(iii): synthesis, characterization, and antimicrobial activity.a series of five new metal complexes of ho(iii) with c-3 substituted derivatives of lawsonemonexime (2-hydroxy-1,4-naphthalenediene-1-oxime) were synthesized. the compounds were characterized by melting point, elemental analysis, ir spectroscopy and magnetic susceptibility. the antimicrobial activity of the compounds were determined by disk diffusion method and broth micro-dilution techniques using mueller hinton medium against the following organisms: s. aureus atcc 6538p, klebsiella pneumoniae ...200018475938
nonpuerperal breast infection.we undertook a microbiological study of purulent specimens from women with symptomatic breast abscesses.199518476022
characterization of antimicrobial resistance and class 1 integrons in enterobacteriaceae isolated from mediterranean herring gulls (larus cachinnans).mediterranean herring gulls (larus cachinnans) were investigated as a possible reservoir of antibiotic resistant bacteria and of cassette-borne resistance genes located in class 1 integrons. two hundred and fourteen isolates of the family enterobacteriaceae were collected from cloacal swabs of 92 chicks captured in a natural reserve in the north east of italy. they showed high percentages of resistance to ampicillin and streptomycin. high percentages of resistance to trimethoprim/sulfamethoxazol ...200818476779
complications of chronic otitis media with cholesteatoma during a 10-year period in kosovo.we review and discuss the results of treatments for complications of cholesteatomatous chronic otitis media (ccom) in a tertiary health care center. in a retrospective study, the medical records of patients with complications of ccom who had undergone surgical treatment at the ent clinic of the university clinical center of kosovo for the period 1994-2004 were reviewed. from a total of 1,803 patients suffering from ccom, in 91 patients, 55 (60.4%) men and 36 (39.6) women, one or two complication ...200818478242
incisional surgical infection in heart transplantation.incisional surgical site infections (issis) are common bacterial infections in heart transplantation (ht). the purpose of this study was to determine the incidence, etiology, timing, and risk factors for issis.200818482203
ulcer infection by esbetal-producing proteus mirabilis: a case report.in this article, a case of decubitus ulcer infection caused by an extended-spectrum beta-lactamase-producing proteus mirabilis strain, successfully treated with oral amoxicillin-clavulanate (1-month therapy) is described. this article focuses on diffusion and clinical effect of extended-spectrum beta-lactamases-producing proteus mirabilis on treatment of gram negative lower extremity infections.200818483010
proteus2: a web server for comprehensive protein structure prediction and structure-based annotation.proteus2 is a web server designed to support comprehensive protein structure prediction and structure-based annotation. proteus2 accepts either single sequences (for directed studies) or multiple sequences (for whole proteome annotation) and predicts the secondary and, if possible, tertiary structure of the query protein(s). unlike most other tools or servers, proteus2 bundles signal peptide identification, transmembrane helix prediction, transmembrane beta-strand prediction, secondary structure ...200818483082
developmental intestinal aerobic microflora in the kori bustard (ardeotis kori).a study was carried out to investigate the normal aerobic bacterial flora of developing kori bustard (ardeotis kori) chicks, captive bred at the national avian research center, abu dhabi, united arab emirates. faecal samples were collected from 14 birds at different ages from the first day of hatching until 99 days old and were cultured for aerobic bacteria. several bacterial species were isolated from the cultures, they included escherichia coli, streptococcus viridians, enterococcus faecalis, ...199818484014
first prize: novel uropathogen-resistant coatings inspired by marine mussels.success in the prevention of urinary device infections has been elusive, largely due to multiple bacterial attachment strategies and the development of urinary conditioning films. we investigated a novel anti-fouling coating consisting of mussel adhesive protein mimics conjugated to polyethylene glycol (mpeg-dopa(3)) for its potential to resist conditioning film formation and uropathogen attachment in human urine.200818484883
dissemination of the ctx-m-25 family beta-lactamases among klebsiella pneumoniae, escherichia coli and enterobacter cloacae and identification of the novel enzyme ctx-m-41 in proteus mirabilis in israel.the ctx-m-25 family of beta-lactamases is a closely related family of enzymes found rarely in the world. we aimed to describe the occurrence and to understand the dissemination of this extended-spectrum beta-lactamase family among enterobacteriaceae strains in our hospital.200818487232
henoch-schönlein purpura associated with proteus mirabilis urinary tract infection. 200818489061
enterobacteriaceae and related organisms isolated from shell eggs collected during commercial processing.in the united states, commercial shell eggs are washed and graded before retail. since passage of the egg products inspection act in 1971, processing guidelines have been set to ensure that external and internal characteristics are maintained. however, less is known about how commercial processing affects the safety of shell eggs. to identify enteric bacteria entering plants and persisting throughout processing, eggs were collected from 3 us commercial shell egg-processing plants on 3 separate v ...200818493013
evolution of bacterial susceptibility pattern of escherichia coli in uncomplicated urinary tract infections in a country with high antibiotic consumption: a comparison of two surveys with a 10 year interval.for the empirical treatment of cystitis, clinicians are often guided by susceptibility data taken from urinary samples that sent to regional microbiological laboratories, which are not representatives for uncomplicated urinary tract infections (utis). to offer adequate recommendations, the distribution and susceptibility pattern of uropathogens in uncomplicated utis in women were compared with those obtained 10 years ago in our uropathogen surveillance in a primary healthcare setting.200818499768
real-time pcrs and fingerprinting assays for the detection and characterization of salmonella genomic island-1 encoding multidrug resistance: application to 445 european isolates of salmonella, escherichia coli, shigella, and proteus.salmonella genomic island-1 (sgi-1) harbors a cluster of genes encoding multidrug resistance (mdr). sgi-1 is horizontally transmissible and is therefore of significant public health concern. this study presents two novel realtime pcrs detecting three sgi-1 protein-coding genes and a sgi-1 fingerprinting assay. these assays were applied to 445 european enterobacterial isolates. results from real-time pcrs were comparable to those obtained from gelbased pcrs used for the detection of sgi-1, but we ...200818500919
surveillance study in europe and brazil on clinical aspects and antimicrobial resistance epidemiology in females with cystitis (aresc): implications for empiric therapy.uncomplicated cystitis in females is among the most frequent infections in community.200818511178
tigecycline in the treatment of complicated intra-abdominal and complicated skin and skin structure infections.tigecycline, a glycylcycline related to the tetracycline class of antibiotics, represents a new option for the treatment of complicated intra-abdominal and complicated skin and skin structure infections. it displays favorable activity in vitro against the most common causative gram-positive, gram-negative and anaerobic pathogens. in addition, tigecycline demonstrates activity against drug-resistant pathogens such as methicillin-resistant staphylococcus aureus, vancomycin-resistant enterococci, a ...200718516315
[influence of the mixed inoculum on the species-identification and antimicrobial susceptibility test results by the automated microbial systems].the influence of the mixed inoculum on the species-identification and antimicrobial susceptibility test results was evaluated for the three automated microbial systems, walkaway-40 (dade microscan, west sacramento, ca, usa), vitek 2 compact (biomérieux, marcy l'etoile, france) and raisus (nissui pharmaceutical, tokyo). in the evaluation, two different species or two different strains were mixed in serial ratios and adjusted to the inoculum cell suspension for the respective systems, and then tes ...200818516962
[in-vitro activity of panipenem against clinical isolates in 2006].the antimicrobial activity of various antibiotics against clinical bacterial isolates recovered from patients with infectious diseases at the medical facilities in the kanto region between march and september 2006 was evaluated. a total of 1030 clinical isolates were available for susceptibility tests: 420 aerobic gram-positive organisms, 520 aerobic gram-negative organisms, 30 anaerobic gram-positive organisms and 60 anaerobic gram-negative pathogens. antimicrobial susceptibility data for strep ...200818536215
detection of a lysozyme inhibitor in proteus mirabilis by a new reverse zymogram method.a reverse zymogram method for the detection of bacterial lysozyme inhibitors was developed. this method was validated by using a periplasmic protein extract of escherichia coli containing a known inhibitor and subsequently led to the detection of a new proteinaceous hen egg white lysozyme inhibitor in proteus mirabilis.200818539812
contemporary management of uncomplicated urinary tract infections.uncomplicated urinary tract infections (uutis) are common in adult women across the entire age spectrum, with mean annual incidences of approximately 15% and 10% in those aged 15-39 and 40-79 years, respectively. by definition, utis in males or pregnant females and those associated with risk factors known to increase the risk of infection or treatment failure (e.g. acquisition in a hospital setting, presence of an indwelling urinary catheter, urinary tract instrumentation/interventions, diabetes ...200818547131
observations on the development of the crystalline bacterial biofilms that encrust and block foley catheters.the care of many patients undergoing long-term bladder catheterisation is complicated when the flow of urine through the catheter is blocked by encrustation. the problem results from infection by urease-producing bacteria, especially proteus mirabilis, and the subsequent formation of crystalline biofilms on the catheter. the aim of this study was to discover how p. mirabilis initiates the development of these crystalline biofilms. the early stages in the formation of the biofilms were observed o ...200818550219
afebrile emphysematous pyelonephritis. 200818550410
depolymerization of chondroitin c sulfate by immobilized proteus vulgaris cells.chondroitinase abc catalyzing the depolymerization of chondroitin sulfate was induced by incubating the proteus vulgaris cells in a medium containing chondroitin c sulfate as an inducer. incubation of p. vulgaris cells for 12 h in the presence of 0.3% inducer was optimal to obtain the cells with highly active chondroitinase abc. such cells were immobilized in k-carrageenan gel lattice, and some properties of chondroitinase abc in immobilized cells were studied in comparison with those of the enz ...198618555284
the kinetics of growth of proteus vulgaris in nicotinic acid-limited cultures.proteus vulgaris is shown to take up nicotinic acid in the early stage of growth in amounts greater than needed for growth. the time variation of the specific nicotinic acid content of the cells, calculated by dividing the amount of nicotinic acid taken up from the liquid medium by the mass of cells present at that time, is interpreted to define two parameters, the maximum specific nicotinic acid content, f(1), and the minimum content required for growth, f(2) the difference, e, between these pa ...198718561122
optimal probe length and target location for electrochemical detection of selected uropathogens at ambient temperature.we have previously demonstrated the clinical validity of the rapid detection of uropathogens by use of a dna biosensor. this assay involves the hybridization of capture and detector probe pairs with bacterial 16s rrna target molecules to form a dna-rna sandwich on the sensor surface. horseradish peroxidase-conjugated antibody binds to the detector probe to enzymatically amplify the hybridization signal. these previous studies involved the hybridization of bacterial 16s rrna target sequences with ...200818562584
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